Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| regulation of phenol degradation in pseudomonas putida. | in order to characterize the ability of pseudomonas putida (trevisan 1889) migula 1895 strain h to degrade various mono- and diphenolic aromatic compounds, respiratory activities towards phenol, catechol, and the cresol isomers were determined. the following rates of oxygen uptake (qo2) were obtained with resting phenol-grown cells: phenol -- 229, o-cresol -- 231, m-cresol -- 43, p-cresol -- 200, catechol -- 262. all these compounds were oxidized by a two-phase-kinetics, the first phase is chara ... | 1981 | 7293241 |
| magnetic and natural circular dichroism spectra of cytochgromes p-450(11) beta and p-450scc purified from bovine adrenal cortex. | magnetic (mcd) and natural circular dichroism (cd) spectra various complexes of cytochrome p-450(11) beta (p-450(11) beta) and cytochrome p-450scc (p-450scc) from bovine adrenal cortex were measured from 250 nm to 700 nm. mcd and cd spectral contours of cytochromes p-450(11) beta and p-450scc in the soret and visible regions were, as a whole, analogous to those of cytochromes p-450 from rabbit liver microsomes and also from pseudomonas putida in their high-spin ferric, high-spin ferrous and ferr ... | 1981 | 7295771 |
| purification of a branched-chain keto acid dehydrogenase from pseudomonas putida. | we purified branched-chain keto acid dehydrogenase to a specific activity of 10 mumol/min per mg of protein from pseudomonas putida grown on valine. the purified enzyme was active with 2-ketoisovalerate, 2-ketoisocaproate, and 2-keto-3-methylvalerate in a ratio of 1.0:0.8:0.7 but showed no activity with either pyruvate or 2-ketoglutarate. there were four polypeptides in the purified enzyme (molecular weights, 49,000, 46,000, 39,000, and 37,000). the purified enzyme was deficient in the specific ... | 1981 | 7298579 |
| 2,3-dihydroxybenzoate pathway in pseudomonas putida. 1h n.m.r. study on the ring-cleavage site. | 1. ring cleavage of 2,3-dihydroxybenzoate by cell-free extracts of pseudomonas putida leads to 2-hydroxy-6-oxo-(2z,4e)-hexa-2,4-dienoic acid and co2. 2. the 1h n.m.r. spectrum of the ring-fission product obtained in a 2h2o solution suggests that the extra-diol cleavage occurs between c-3 and c-4. | 1981 | 7306005 |
| mössbauer spectroscopic studies of the terminal dioxygenase protein of benzene dioxygenase from pseudomonas putida. | mössbauer spectra obtained from the terminal dioxygenase protein of the benzene dioxygenase system from pseudomonas putida show that it contains [2fe--2s] centres similar to those of the two-iron plant-type ferredoxins. in the oxidized form the two iron atoms within the centre are high-spin ferric but with considerable inequivalence. in the reduced form the centre contains one extra electron, and this is localized on one of the iron atoms, which becomes high-spin ferrous. | 1981 | 7306045 |
| the reaction of oxygen with protocatechuate 3,4-dioxygenase from pseudomonas putida. characterization of a new oxygenated intermediate. | the reactions of protocatechuate dioxygenase (protocatechuate:oxygen 3,4-oxidoreductase, ec 1.13.11.3) with substrates and oxygen have been studied at 4 degrees c using rapid kinetic techniques. in this study, two oxygenated intermediates were kinetically and spectrally characterized. the rate of oxygen addition to the enzyme-substrate complex was determined to be 5 x 10(5) m-1 s-1. this oxygenated complex rapidly converts (450 s-1) to another spectrally identifiable compound which then breaks d ... | 1981 | 7309730 |
| participation of the beta-ketoadipate transport system in chemotaxis. | beta-ketoadipate serves as a chemoattractant for pseudomonas putida. the chemotactic response is inducible, and a regulatory mutant strain that forms the beta-ketoadipate transport system at high levels exhibits a heightened chemotactic response to beta-ketoadipate. adipate and succinate, compounds that interact with the transport system, inhibit chemotaxis toward beta-ketoadipate. some, but not all, mutants that fail to respond chemotactically to beta-ketoadipate lack the beta-ketoadipate trans ... | 1981 | 7320700 |
| [evaluation of biological activity of l-methionidase from pseudomonas putida ac-75 on the basis of in vitro studies]. | 1981 | 7342702 | |
| reconstitution of iron-sulfur cluster of nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1. | nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system, is an ion-sulfur flavoprotein with one fad and one iron-sulfur cluster of [2fe-2s] type (yamaguchi, m., and fujisawa, h. (1978) j. biol. chem. 253, 8848-8853). treatment of nadh-cytochrome c reductase with p-chloromercuriphenylsulfonic acid resulted in fading of its color with a concomitant loss of the nadh-cytochrome c reductase activity. the p-chloromercuriphenylsulfonic acid-treated enzyme was found to contain one fa ... | 1981 | 7240244 |
| purification and properties of nadh-ferredoxintol reductase. a component of toluene dioxygenase from pseudomonas putida. | cells of pseudomonas putida, after growth with toluene, contain a multicomponent enzyme system that oxidizes toluene to (+)-1(s),2(r)-dihydroxy-3-methyl-cyclohexa-3,5-diene. one of these components has been purified to homogeneity and shown to be a flavoprotein that contains fad as the only detectable prosthetic group. fad was removed from the enzyme during purification. however, equilibrium dialysis experiments showed that the enzyme can bind one mol of fad/mol of enzyme protein. the apparent m ... | 1981 | 7204373 |
| mechanism of salicylate hydroxylase-catalyzed decarboxylation. | salicylate hydroxylase (salicylate, nadh: oxygen oxidoreductase (1-hydroxylating, decarboxylating), ec 1.14.13.1) in pseudomonas putida catalyzed hydroxylation of the substrate analogue, salicylaldehyde, to form catechol and formate with stoichiometric consumption of nadh and o2. consequently, a study of primary product derived from the carboxyl group of the authentic substrate, salicylate, was undertaken. the experimental results revealed that co2 not h2co3, was produced first. | 1981 | 7213760 |
| catabolism of pseudocumene and 3-ethyltoluene by pseudomonas putida (arvilla) mt-2: evidence for new functions of the tol (pwwo) plasmid. | pseudocumene (1,2,4-trimethylbenzene) and 3-ethyltoluene were found to serve as growth substrates for pseudomonas putida (arvilla) mt-2, in addition to toluene, m-xylene, and p-xylene as previously described. similar observations were made with several additional p. putida strains also capable of growth with toluene and the xylenes. additional substrates which supported the growth of these organisms included 3,4-dimethylbenzyl alcohol, 3,4-dimethylbenzoate, and 3-ethylbenzoate. p. putida mt-2 ce ... | 1981 | 7216999 |
| plasmid specifying total degradation of 3-chlorobenzoate by a modified ortho pathway. | a plasmid, termed pac25, specifying biodegradation of 3-chlorobenzoate in a strain of pseudomonas putida has been characterized. during growth of the plasmid-harboring cells with 3-chlorobenzoate, there was an accumulation of 3-chlorocatechol and beta-chloromuconic acid as intermediates and release of more than 80% of the chlorine in the form of inorganic chloride. the plasmid had a mean molecular mass of 68 x 10(6) daltons and was transmissible to a number of pseudomonas species such as p. aeru ... | 1981 | 7217013 |
| [arsenic oxidation by the heterotrophic bacteria pseudomonas putida and alcaligenes eutrophus]. | two heterotrophic bacteria, pseudomonas putida 18 and alcaligenes eutrophus 280, were isolated from gold-arsenic deposits. the bacteria oxidize as(iii) to as(v) at ph 6-9 and temperatures 4-28 and 28 degrees c respectively. the oxidation is accompanied by a decrease in the ph of the medium. the rate of the oxidation directly depends on the number of cells in the inoculum. | 1981 | 7219219 |
| [the identification of nonfermentative gram-negative bacteria. experiences with 676 apyocyaninogenic strains (author's transl)]. | during a period of 16 months 1757 strains of nonfermentative gram-negative rods have been isolated from clinical material. of the, 1205 (69%) were p. aeruginosa, 124 (10%) of which failed to produce pyocyanin. the apyocyaninogenic strains as well as the remaining 552 isolates were differentiated by steps according to a diagnostic scheme developed by us. for identification of species two or three steps were needed. by this procedure, 530 of the 552 strains could be assigned to nineteen species wi ... | 1981 | 7223132 |
| isolation and characterization of spontaneously occurring tol plasmid mutants of pseudomonas putida hs1. | a strain of pseudomonas (p. putida hs1) was found to resemble p. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. the ability to degrade these substrates by p. putida hs1 (ppc1) was shown to be encoded by a tol (pdk1) plasmid as evidenced by: (i) spontaneous loss of the tol-related phenotype after growth with benzoate, (ii) transfer o ... | 1981 | 7240090 |
| interaction of 5-bromocamphor with cytochrome p-450 cam. production of 5-ketocamphor from a mixed spin state hemoprotein. | camphor is stereospecifically hydroxylated by the soil bacterium pseudomonas putida at the 5-exo position by a cytochrome p-450 mixed function oxidase system consisting of a flavoprotein reductase; putidaredoxin, an iron-sulfur oxidation-reduction transport-effector protein; and the p-450 hemoprotein. we have studied the interaction of a substrate analog of camphor, 5-exo-bromocamphor, with this cytochrome p-450 mixed function oxidase system in order to probe the molecular mechanisms of electron ... | 1981 | 7240237 |
| 8 alpha-(o-tyrosyl)flavin adenine dinucleotide, the prosthetic group of bacterial p-cresol methylhydroxylase. | 8 alpha-(o-tyrosyl)riboflavin has been synthesized by condensation of the copper complex of l-tyrosine with 8 alpha-bromotetraacetylriboflavin. the structure of this synthetic product was proven by absorption and 1h nmr spectroscopy and by chemical degradation, which yielded 1 mol of tyrosine per mol of flavin. the synthetic compound comigrated wtih the (aminoacyl)riboflavin isolated from the p-cresol methylhydroxylase of pseudomonas putida, and both showed identical absorption and fluorescence ... | 1981 | 7248267 |
| symbiotic utilization of polyvinyl alcohol by mixed cultures. | polyvinyl alcohol (pva)-utilizing cultures were obtained from various sources. they were mixed cultures even after cyclical transfer to liquid and plate media with pva as a sole source of carbon. component bacteria were isolated from the several mixed cultures, and it was shown that pva was utilized symbiotically by two bacterial members which could not utilize pva in each respective pure culture. from a mixed culture, strains vm15, vm15a (pseudomonas putida) and vm15c (pseudomonas sp.) were iso ... | 1981 | 16345693 |
| biological process for converting naphthalene to cis-1,2-dihydroxy-1,2-dihydronaphthalene. | a biological process for converting naphthalene to cis-1,2-dihydroxy-1,2-dihydronaphthalene (dhd) catalyzed by pseudomonas putida strain 119 was optimized in flask experiments. these studies revealed the following: (i) p. putida 119 can propagate efficiently and produce dhd when supplied one of several carbon sources and naphthalene; (ii) maximum dhd production by p. putida 119 occurs in logarithmic-growth-phase cells and decreases at various rates in the stationary growth phase, depending upon ... | 1980 | 16345504 |
| separation of d-(+)-nicotine from a racemic mixture by stereospecific degradation of the l-(-) isomer with pseudomonas putida. | incubation of racemic mixtures of dl-(+/-)-nicotine with pseudomonas putida resulted in a complete stereoselective degradation of the l-(-) isomer. unnatural d-(+)-nicotine, which is of pharmacological interest for stereochemical studies of various nicotine-responsive systems, was not affected by the bacterium and was recovered by extraction. | 1980 | 16345568 |
| d-glucose and d-gluconate transport in vesicles from pseudomonas putida. | vesicles prepared from glucose-grown cells of pseudomonas putida (atcc, 12633) retain glucose oxidase (gox) and gluconate dehydrogenase (gadh) activity and actively transport d-glucose, 2-deoxy-d-glucose (2dog), 3-deoxy-3-fluoro-d-glucose (3fg), and d-gluconate by saturable processes. the transport of these substrates is stimulated by the addition of l-malate or reduced phenazine methosulphate (pms). vesicles prepared from succinate-grown cells of p. putida lose their capacity to transport d-glu ... | 1980 | 7248836 |
| biological distribution and physiological role of the beta-ketoadipate transport system. | beta-ketoadipate induces catabolic enzymes in pseudomonas putida. the compound is transported by a system which also concentrates adipate, a non-metabolizable analogue of beta-ketoadipate. the natural substrate, beta-ketoadipate, competitively inhibits adipate transport with a k1 of 0.04 mm, lower than the km of 0.23 mm observed with adipate. transport is inhibited competitively by succinate (k1 1.3 mm) and non-competitively by acetate (k1 5.3 mm). the system has a sharp ph optimum at 5.5. trans ... | 1980 | 7217919 |
| digestion of algin by pseudomonas maltophilia and pseudomonas putida. | pseudomonas maltophilia and pseudomonas putida were identified as alginolytic species. two media used for demonstrating alginolytic activity are described. the applied aspects of the ability of these two species to digest algin are discussed. | 1980 | 7356324 |
| the purification and characterization of delta 5-3-ketosteroid isomerase from pseudomonas putida, a cysteine-containing isomerase. | a delta 5-3-ketosteroid isomerase (ec 5.3.3.1) has been isolated from pseudomonas putida biotype b and purified to homogeneity. this previously undescribed steroid isomerase resembles that isolated from pseudomonas testosteroni (talalay, p., and wang, v.s. (1955) biochim. biophys. acta 18, 300-301). the enzyme is induced by various steroids, has a subunit molecular weight of 13,750 +/- 250, a pi of 4.8 +/- 0.1 has a specific activity of 40,000 units/mg, using 5-androstene-3,17-dione as the subst ... | 1980 | 7358699 |
| kinetics of the isomerization of 5-androsten-3,17-dione catalyzed by delta 5-3-ketosteroid isomerase from pseudomonas putida. | 1980 | 7358700 | |
| active site-directed photoinactivation of delta 5-3-ketosteroid isomerase from pseudomonas putida dependent on 1,4,6-androstatrien-3-one-17 beta-ol. | delta 5-3-ketosteroid isomerase from pseudomonas putida is subject to photoinactivation by light of wavelengths greater than 300 nm, specifically in the presence of the competitive inhibitor, 1,4,6-androstatrien-3-one-17 beta-ol (teo). in the absence of this steroid or in the presence of the nonchromophoric steroidal competitive inhibitor, deoxycholate, the enzyme activity is essentially unaffected by irradiation. deoxycholate protects the enzyme from the teo-dependent reaction to a degree which ... | 1980 | 7358701 |
| purification and characterization of an oxygenase component in benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1. | the benzoate 1,2-dioxygenase system of pseudomonas arvilla consists of two proteins, an nadh-cytochrome c reductase and an oxygenase. the oxygenase component was purified to apparent homogeneity by the criteria of polyacrylamide gel electrophoresis from benzoate-induced cells of p. arvilla. the molecular weight of the enzyme was determined to be 273,000 by sedimentation equilibrium analysis, 280,000 by electrophoresis on polyacrylamide gels of different concentrations, and 270,000 by sepharose c ... | 1980 | 7372624 |
| energetics of myo-inositol transport in pseudomonas putida. | the effects of specific inhibitors on the high-affinity transport system of cyclitols and on the respiration of pseudomonas putida shows that the transport activity is dependent on high energy phosphate bond. | 1980 | 7379940 |
| hybrid pathway for chlorobenzoate metabolism in pseudomonas sp. b13 derivatives. | derivatives of pseudomonas sp. b13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the tol plasmid of pseudomonas putida mt-2 were studied. the utilization of these substrates, a property not shared by the parent strains, was shown to depend upon the combined activities of enzymes from the donor and from the recipient. during growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate, predominantly the toluate 1,2-deo ... | 1980 | 7380800 |
| production of methanol from aromatic acids by pseudomonas putida. | when grown at the expense of 3,4,5-trimethoxybenzoic acid, a strain of pseudomonas putida oxidized this compound and also 3,5-dimethoxy-4-hydroxybenzoic (syringic) and 3,4-dihydroxy-5-methoxybenzoic (3-o-methylgallic) acids; but other hydroxy- or methoxy-benzoic acids were oxidized slowly or not at all. radioactivity appeared exclusively in carbon dioxide when cells were incubated with [4-methoxyl-14c]trimethoxybenzoic acid, but was found mainly in methanol when[methoxyl-14c]3-o-methylgallic aci ... | 1980 | 7380811 |
| a simple method for the preparation of d-alpha-aminoadipic acid. | high quantity (1 g and more) of racemically and chromatographically pure d-alpha-aminoadipic acid was prepared by selective metabolism of the l-isomer of the commercially available dl-alpha-aminoadipate by pseudomonas putida. the overall yield of this preparation averaged 40%. the final product has [a]25d value of -25 degrees. this procedure can be useful in the synthesis of high purity d-alpha-amino-adipate, a compound shown recently to be a useful tool in the study of neurotransmission mechani ... | 1980 | 7383979 |
| repetitions in the nh2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from pseudomonas putida. | muconolactone delta-isomerase (ec 5.3.3.4) and beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) mediate consecutive reactions in the beta-ketoadipate pathway of bacteria. an earlier investigation (yeh, w.k., davis, g., fletcher, p., and ornston, l.n. (1978) j. biol. chem. 253, 4920-4923) revealed that the respective nh2-terminal amino acid sequences of pseudomonas putida muconolactone isomerase and acinetobacter calcoaceticus beta-ketoadipate enol-lactone hydrolase ii are evolutionarily hom ... | 1980 | 7391022 |
| homologies in the nh2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases. | gamma-carboxymuconolactone decarobxylase (ec 4.1.1.44) and muconolactone isomerase (ec 5.3.3.4) mediate chemically analogous reactions in bacteria. the enzymes are inducible, and different metabolites trigger the respective syntheses of the decarboxylases in acinetobacter calcoaceticus and pseudomonas putida. the decarobxylases share similar oligomeric structures in which identical subunits of about 13,300 daltons appear to be self-associated into hexamers. identical residues are found in 18 of ... | 1980 | 7391024 |
| [microbiological transformation of quinoline by pseudomonas putida bacteria]. | 1980 | 7402102 | |
| [electrokinetic properties of arthobacter siderocapsulatus]. | the electro-kinetic properties of arthrobacter siderocapsulatus were determined using microelectrophoresis in alternating electric field. the surface of bacterial cells was shown to bear positive charge within a wide range of ph. the positive charge of the cells should be attributed apparently to the presence of mucous capsules containing manganese dioxide since the growth of the bacterium is known to involve oxidation of manganese or ferrous iron and accumulation of their hydroxides in the caps ... | 1980 | 7402120 |
| degradation of (-)-ephedrine by pseudomonas putida. detection of (-)-ephedrine: nad+-oxidoreductase from arthrobacter globiformis. | a bacterium utilizing the alkaloid (-)-ephedrine as its sole source of carbon was isolated by an enrichment-culture technique from soil supplemented with 4-benzoyl-1,3-oxazolidinon-(2). the bacterium was indentified as pseudomonas putida by morphological and physiological studies. the following metabolites were isolated from the culture fluid: methylamine, formaldehyde, methylbenzoylcarbinol (2-hydroxy-1-oxo-1 phenylpropane), benzoid acid, pyrocatechol and cis, cis-muconic acid. a pathway for th ... | 1980 | 7405363 |
| cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from pseudomonas ovalis. | the cd-, cr-, and mn-substituted enzymes of iron-superoxide dismutase from pseudomonas ovalis were prepared from the apoenzyme by using the alkaline treatment method described before (1) with a slight modification. the cd-substituted enzyme had 1.16 g atoms of cd per mol of enzyme and no visible absorption. the cr-substituted enzyme had 1.27 g atoms of cr per mol of enzyme and had absorption maxima at 530 nm and 670 nm with a shoulder around 370 nm. the mn-substituted enzyme had 1.27 g atoms of ... | 1980 | 7410333 |
| enzymological aspects of caffeine demethylation and formaldehyde oxidation by pseudomonas putida c1. | 1) the enzymatic demethylation of caffeine (1,3,7-trimethylxanthine) by pseudomonas putida c1 was investigated; an inducible enzyme system has been observed. this enzyme shows an optimum ph of about 6.0, and the optimum temperature is in the range of 22-24 degrees c. the enzyme is absolutely dependent on nadh or nadph as a cosubstrate and is activated by co2+. 2) the formaldehyde generated by the demethylation of caffeine is oxidized by an nad-dependent formaldehyde dehydrogenase, which is indep ... | 1980 | 7461603 |
| naphthalene metabolism by pseudomonads: purification and properties of 1,2-dihydroxynaphthalene oxygenase. | 1,2-dihydroxynaphthalene oxygenase was purified from pseudomonas putida ncib 9816 grown on naphthalene as the sole source of carbon and energy. the enzyme had a subunit molecular weight of 19,000 and in a medium containing phosphate buffer, 1 mm mercaptoethanol, and 10% (vol/vol) ethanol had a native molecular weight greater than 275,000. the enzyme required fe2+ for activity. it was inactivated slowly on standing, and inactivation was accelerated by dilution with aerated buffers and by h2o2. ba ... | 1980 | 7204331 |
| p-cymene pathway in pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs. | several classes of mutants of pseudomonas putida (jt810) defective in the utilization of p-cymene as sole carbon source have been isolated. selective enrichment of the mutants and for strains putatively cured of a degradative plasmid was achieved by incubation of cells in minimal growth media containing p-cymene (or p-cumate) and various halogenated analogs of the growth substrates or pathway intermediates. analogs which led to successful enrichments included: p-chlorotoluene, p-bromotoluene, al ... | 1980 | 7204334 |
| purification of glutamine synthetase from a variety of bacteria. | we have developed two procedures which allow the very rapid purification of glutamine synthetase (gs) from a diverse variety of bacteria. the first procedure, based upon differential sedimentation, depends upon the association of gs with deoxyribonucleic acid in cell extracts. the second procedure, derived from the method of c. gross et al (j. bacteriol. 128:382-389, 1976) for purifying ribonucleic acid polymerase by polyethylene glycol (peg) precipitation, enabled us to obtain high yields of gs ... | 1980 | 6102984 |
| the conformation of catalytically functional schiff's bases: the pyruvate--lysine ketimine of 2-keto-3-deoxygluconate-6-phosphate aldolase of pseudomonas putida. | 1980 | 6109521 | |
| substrate-mediated inactivation of urocanase from pseudomonas putida. evidence for an essential sulfhydryl group. | incubation of urocanase from pseudomonas putida with either its substrate, urocanic acid, or product, 4'(5')-imidazolone-5'(4')-propionic acid, resulted in an oxygen-dependent inhibition of enzyme activity. coincident with the inactivation was the stoichiometric incorporation of radioactivity from [14c]urocanate into the protein. nad+ which is required for activity or urocanase was not directly involved in the inactivation process. the inactivation of urocanase was irreversible, could be partial ... | 1980 | 6109547 |
| expression of the lactose transposon tn951 in escherichia coli, proteus and pseudomonas. | the control of beta-galactosidase specified by the lactose transposon tn951 (inserted into rp1 to give pgc9114) has been studied in escherichia coli k12, proteus mirabilis, pseudomonas aeruginosa and pseudomonas putida; in the first two species comparison could be made with flac. in e. coli k12, the tn951 and chromosomally encoded enzymes showed marked qualitative differences in regulatio, the former giving a substantially lower maximum induced level and induction ratio. several parameters were ... | 1980 | 6251161 |
| [plasmid characteristics of naphthalene and salicylate biodegradation in pseudomonas putida]. | the object of this work was to study the physico-chemical and biological properties of dnas of the biodegradation plasmids nah and sal. a comparative analysis of the physico-chemical parameters for these dnas made it possible to detect a number of identical properties in them: the same sedimentation profile for covalently-closed circular dna forms, 68--70 s; the molecular weight of ca. 50 md; a roughly equal number of fragments (up to 23) was found when the dnas of nah and sal were restricted by ... | 1980 | 6259498 |
| [phage pf16 interrelationships with pseudomonas putida bacteria. i. unstable transductants and mutants of pseudomonas putida pfg1 resistant to phage pf16]. | the frequencies of transduction of the chromosomal genes by pf16 in pseudomonas putida pgg1 are dependent on the marker transduced and unpredictable. histidine and isoleucine-valine positive transductants, which are resistant to pf16, have been selected in the crosses with low (about 10(-8) for phage units) frequencies of transduction. some of these transductants carry new mutations. the transductants are unstable for phage resistance and histidine or isoleucine-valine positive characters. there ... | 1980 | 6928126 |
| construction of a partial diploid for the degradative pathway encoded by the tol plasmid (pwwo) from pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyir gene. | 1980 | 6929031 | |
| primary structure of serratia marcescens anthranilate synthase component ii. | the amino acid sequence of anthranilate synthase component ii (as ii) from serratia marcescens was determined. the cysteine residue essential for glutamine utilization was alkylated selectively by iodo [1-14c]acetamide prior to separation of the two protein components of anthranilate synthase. the isolated as ii then was subjected to cleavage by cyanogen bromide and by trypsin after citraconylation to obtain overlapping fragments. as ii is a single polypeptide chain of 192 residues having a calc ... | 1980 | 6986371 |
| regulation of enzymes of the 3,5-xylenol-degradative pathway in pseudomonas putida: evidence for a plasmid. | constitutive synthesis of enzymes responsible for methyl group oxidation in 3,5-xylenol degradation and an associated p-cresol methylhydroxylase in pseudomonas putida ncib 9869 was shown by their retention at high specific activities in cells transferred from 3,5-xylenol medium to glutamate medium. the specific activities of other enzymes of the 3,5-xylenol pathway declined upon removal of aromatic substrate, consistent with their inducible control. specific activities of the methyl-oxidizing en ... | 1980 | 6989805 |
| [nucleoside catabolism study of pseudomonas putida and pseudomonas trifolii]. | no activity of specific nucleoside phosphorylases was detected in pseudomonas putida; as the result, its cells cannot grow on various nucleosides using them as carbon sources. insignificant growth on ribonucleosides, viz. inosine and uridine, should be attributed to the activity of hydrolases in the cell. in contrast, pseudomonas trifolii is capable of normal growth on various nucleosides using them as a sole source of carbon. accordingly, the cell extracts display the activities of enzymes invo ... | 1980 | 6993879 |
| effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography. | high-resolution pyrolysis gas-liquid chromatography was applied to three bacteria (escherichia coli nctc 9001, pseudomonas putida (ncib 9494, and staphylococcus aureus nctc 8532) grown under a variety of conditions. changing the culture medium drastically altered the quantitative aspects of the pyrograms of all three organisms, but the effects of culture time and incubation temperature were less severe. mathematical analysis of the relative peak heights showed that four peaks could be used to di ... | 1980 | 6999989 |
| chemical structure and inhalation toxicity of lipopolysaccharides from bacteria on cotton. | lipopolysaccharides from different bacteria isolated from cotton were purified and chemically analyzed. their pulmonary toxicity to animals was tested in inhalation tests. lipopolysaccharides from agrobacterium and xanthomonas were shown to differ from the others in that they contained no heptose and no non-hydroxylated fatty acids with a chain length of 12, 14, or 16 carbon atoms. lipopolysaccharides from pseudomonas putida, enterobacter agglomerans, and klebsiella oxytoca were found to cause a ... | 1980 | 7000706 |
| mutants defective in isomerase and decarboxylase activities of the 4-hydroxyphenylacetic acid meta-cleavage pathway in pseudomonas putida. | degradation of 2-hydroxy-5-carboxymethylmuconic semialdehyde, the ring fission product of the 4-hydroxyphenylacetate meta-cleavage pathway, by mutant strains p23x19 and p23x16 of pseudomonas putida nci b 9865 was studied. both mutants were unable to grow on either 4-hydroxyphenylacetate of 3,4-dihydroxyphenylacetate. cell extracts of p23x19, grown in the presence of 3,4-dihydroxyphenylacetate, degraded the ring fission product to a compound that accumulated and had maximum uv absorption at 300 n ... | 1980 | 6769900 |
| reactivation studies on putidamonooxin -- the monooxygenase of a 4-methoxybenzoate o-demethylase from pseudomonas putida. | 1980 | 6772176 | |
| headspace analysis of volatile metabolites of pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry. | gas chromatographic-mass spectrometric analysis of headspace volatiles was performed on cultures of 11 strains of pseudomonas aeruginosa and 1 strain each of pseudomonas cepacia, pseudomonas putida, pseudomonas putrefaciens, pseudomonas fluorescens, and pseudomonas maltophilia. all strains of pseudomonas aeruginosa produced a distinctive series of odd-carbon methyl ketones, particularly 2-nonanone and 2-undecanone, and 2-aminoacetophenone. the other strains failed to produce 2-aminoacetophenone. ... | 1980 | 6775012 |
| expression of e. coli genes carried by hybrids of plasmid rp4. | hybrids of the rp4 plasmid, containing bacteriophage mu and chromosomal genes of escherichia coli, were transferred into salmonella typhimurium, pseudomonas putida, pseudomonas aeruginosa and proteus mirabilis. the individual genes of the arginine, histidine, leucine and threonine operons were expressed in these microorganisms. | 1980 | 6777258 |
| inhibition, but not uncoupling, of respiratory energy coupling of three bacterial species by nitrite. | the effect of nitrite on respiratory energy coupling of three bacteria was studied in light of a recent report that nitrite acted as an uncoupling agent with paracoccus denitrificans grown under denitrifying conditions. our determinations of proton translocation stoichiometry of pseudomonas putida (aerobically grown), pseudomonas aeruginosa, and p. denitrificans (grown both aerobically and under denitrifying conditions) showed nitrite inhibition of proton-to-oxidant stoichiometry, but not uncoup ... | 1980 | 6777373 |
| comparative immunochemical studies of cytochrome p-450cam of pseudomonas putida and of cytochrome p-450scc of bovine adrenocortical mitochondria. | 1980 | 6780340 | |
| [effect of humic acids from the brown coal of the aleksandriyskoya field on pseudomonas putida growth]. | 1980 | 6446021 | |
| direct hydroxylation in the biosynthesis of hydroxy fatty acid in lipid a of pseudomonas ovalis. | it was found that pseudomonas ovalis iam 1177 had an abundance of hydroxy fatty acids such as 3-hydroxy-decanoic acid, 3-hydroxy-dodecanoic acid and 2-hydroxy-dodecanoic acid in the lipophilic part of the lipopolysaccharide fraction, which comprise 80% of total fatty acids. by using 18o2, it was shown that one oxygen atom from molecular oxygen was incorporated into 2-hydroxy-dodecanoic acid, but not into 3-hydroxy-decanoic acid. the incorporated oxygen atom was specifically located at the hydrox ... | 1979 | 760794 |
| fertility factors in pseudomonas putida: selection and properties of high-frequency transfer and chromosome donors. | the octane plasmid (oct) in pseudomonas putida strains has been shown to be transferred at low frequency. however, bacteria which had newly received this plasmid showed a transient increase in donor ability. using octane+ p. putida as the donor, the transfer of most chromosomal markers was shown to be independent of oct transfer, whereas the mobilization of the octanoate catabolism genes (octanoic and acetate) was dependent on oct plasmid transfer. the presence of a fertility factor termed fpo h ... | 1979 | 762014 |
| dissociation of the nic plasmid aggregate in pseudomonas putida. | the nic plasmid on conjugal transfer from pseudomonas convexa pc 1 to pseudomonas putida ppg 1 dissociates into an independent fertility factor t and a nontransmissible nic structural gene plasmid. | 1979 | 762028 |
| expression of escherichia coli tryptophan operon in rhizobium leguminosarum. | rp4-trp hybrid plasmid containing escherichia coli whole tryptophan operon was conjugatively transferred from e. coli to rhizobium leguminosarum strains carrying mutations in different trp genes, converting their trp- phenotype to trp+. that the phenotype change of the r. leguminosarum cells was due to the presence of the e. coli tryptophan operon was verified by the isolation of rp4-trp hybrid plasmid from the r. leguminosarum conjugant cells, and by re-transfer of rp4-trp plasmid by conjugatio ... | 1979 | 375025 |
| nonfermentative bacilli: evaluation of three systems for identification. | three systems for the identification of nonfermentative bacilli were evaluated for their rapidity and accuracy of identification of 217 strains. two of the systems, api 20e (api) and oxi/ferm tube (oxif), are available as kits; the oxidative attack (oa) system is not commerically available. the overall accuracies of the oa, api, and oxif systems were 91, 69, and 50%, respectively. identification within 48 h was achieved for 98% of the strains by oa, for 50% by api, and for 18% by oxif. most of t ... | 1979 | 389945 |
| formaldehyde dehydrogenase from pseudomonas putida. purification and some properties. | formaldehyde dehydrogenase was isolated and purified in an overall yield of 12% from cell-free extract of pseudomonas putida c-83 by chromatographies on columns of deae-cellulose, deae-sephadex a-50, and hydroxyapatite. the purified enzyme was homogeneous as judged by disc gel electrophoresis and was most active at ph 7.8 using formaldehyde as a substrate. the enzyme was also active toward acetaldehyde, propionaldehyde, glyoxal, and pyruvaldehyde, though the reaction rates were low. the enzyme w ... | 1979 | 571868 |
| stereochemistry of the conversion of methacrylate to beta-hydroxyisobutyrate in pseudomonas putida. | 1979 | 572832 | |
| stereospecific hydrogen loss in the conversion of [2h7]isobutyrate to beta-hydroxyisobutyrate in pseudomonas putida. the stereochemistry of beta-hydroxyisobutyrate dehydrogenase. | 1979 | 573276 | |
| identification of the prosthetic group of urocanase. the mode of its reaction with sodium borohydride and of its photochemical reactivation. | urocanase from pseudomonas putida and from beef liver were isolated by modifying described procedures. both enzymes were inactivated and labeled on treatment with tritiated sodium borohydride and gave, upon subsequent hydrolysis, a radioactive acid. the previously reported identity of this acid as 2-hydroxybutanoic acid was disproved by several criteria. other hydroxy acids were also proved to be different from the radioactive acid derived from urocanase. a large portion of the radioactive mater ... | 1979 | 33176 |
| [expression of deo-operon escherichia coli k-12 genes in the makeup of hybrid plasmid rp4::mu-deo in pseudomonas trifolii and pseudomonas putida]. | 1979 | 113191 | |
| deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms. | plasmid and phage deoxyribonucleic acid (dna) harboring bacterial insertion sequence (is) elements is1, is2, and is5 were characterized and used as probes to detect homologous sequences in various procaryotic and eucaryotic genomes. the hybridization method used permits the detection of sequences partially homologous to the elements. hybridization of the is-containing probes to each other revealed a region of limited homology between is1 and is2. homologous sequences were then detected by comput ... | 1979 | 159291 |
| comparison of microsomal and solubilized monooxygenases from rat and rabbit by proton magnetic relaxation. | the paper presents results of a comparative study of the haem environment, by proton magnetic relaxation, in p-450 and p-448 monooxygenases from rat and rabbit, induced by phenobarbital and 3-methylcholanthrene, in both species. it was established that the method yields information on the accessibility of the haem iron for solvent molecules (protons), both in microsomes and in solubilized samples of various degrees of purification, i.e. association. the state of micelles in the solutions does no ... | 1979 | 229678 |
| a cleavage map of the tol plasmid of pseudomonas putida mt-2. | a cleavage map of the tol plasmid pwwo has been determined for the restriction endonucleases hindiii and xhoi. a number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified xhoi fragments to southern blots of hindiii digest products and (iii) analysis of a number of deletion mutants. | 1979 | 231727 |
| chromosomal mobilization from a reca mutant of pseudomonas putida. | a methyl methane sulfonate sensitive mutant of p. putida strain ppg1 is also extremely sensitive to uv-rays, compared to parent wild type cells. this mutant behaves typically as recombination less (reca) mutants of escherichia coli and pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. sex factor plasmids such as k-xyl or tol can mobilize chromosomal genes equally well bot ... | 1979 | 232230 |
| an endonuclease cleavage map of the plasmid pwwo-8, a derivative of the tol plasmid of pseudomonas putida mt-2. | cleavage sites on the pwwo-8 plasmid were determined for the restriction endonucleases hindiii and xhoi. terminal labelling using dna polymerase i was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasmid. | 1979 | 285316 |
| origins of metabolic diversity: evolutionary divergence by sequence repetition. | recurring patterns of primary structure have been observed in enzymes that mediate sequential metabolic reactions in bacteria. the enzymes, muconolactone delta-isomerase [(+)-4-hydroxy-4-carboxymethylisocrotonolactone delta(2)-delta(3)-isomerase, ec 5.3.3.4] and beta-ketoadipate enol-lactone hydrolase [4-carboxymethylbut-3-enolide(1,4)enol-lactone-hydrolase, ec 3.1.1.24], have been coselected in bacterial populations because the isomerase can confer no nutritional advantage in the absence of the ... | 1979 | 291059 |
| alpha-hydroxyglutarate as an intermediate in the catabolism of alpha-aminoadipate by pseudomonas putida. | 1979 | 429347 | |
| properties of the iron--sulphur proteins of the benzene dioxygenase system from pseudomonas putida. | a purification procedure was developed to stabilize the iron-sulphur proteins of the benzene dioxygenase system from pseudomonas putida. the intermediate electron-carrying protein has a mol. wt. of 12300 and possesses one (2fe--2s) cluster, whereas the terminal dioxygenase has a mol.wt. of 215300 and possesses two (2fe--2s) clusters. the order and stoicheiometry of electron transfer and of the whole system are described. | 1979 | 435241 |
| interaction of the chiral pyruvate analog, 2-keto-3-bromobutyrate, with pyruvate lyases. 2-keto-3-deoxygluconate-6-phosphate aldolase of pseudomonas putida. | the enzyme 2-keto-3-deoxygluconate-6-p aldolase of pseudomonas putida is inactivated by one of the chiral forms of 2-keto-(3rs)-3-bromobutyric acid (bromoketobutyrate). the inactivation shows saturation kinetics and competition with pyruvate. the minimal inactivation half-time is 4 min and that concentration of bromoketobutyrate half-saturating the enzyme is 2 mm. (3rs)-[3-3h]bromoketobutyrate is catalytically detritiated during enzyme inactivation. a kinetic analysis of rates gave data consiste ... | 1979 | 447683 |
| raman spectrum of protocatechuate dioxygenase from pseudomonas putida. new low frequency bands. | 1979 | 454431 | |
| structural comparison of gamma-carboxymuconolactone decarboxylase and muconolactone isomerase from pseudomonas putida. | 1979 | 454663 | |
| a theoretical interpretation of the variations of some physical parameters within the [2fe-2s] ferredoxin group. | a model is proposed to explain the variation of some physical parameters within the reduced [2fe-2s] ferredoxin group. according to this model, the main effects result from a variable mixing of some d orbitals of the fe2+ ion owing to rhombic distortion of the active site having the same geometrical character, but different in intensity, for each protein. some peculiar experimental results such as the axial electron paramagnetic resonance spectra of adrenal ferredoxin and pseudomonas putida ferr ... | 1979 | 465523 |
| nonidentical subunits of pyrocatechase from pseudomonas arvilla c-1. | 1979 | 475379 | |
| transport of c5 dicarboxylate compounds by pseudomonas putida. | induced glutarate and 2-oxoglutarate uptake and transport by pseudomonas putida were investigated in whole cells and membrane vesicles, respectively. uptake of 2-oxoglutarate, but not glutarate, was against a concentration gradient to 1.7-fold greater than the initial extracellular concentration. membrane vesicles transported 2-oxoglutarate and glutarate against gradients to intramembrane concentrations fivefold greater than the initial extravesicle concentrations. the rates of transport of both ... | 1979 | 479107 |
| autoradiographic study of the localization and evolution of growth zones in bacterial colonies. | incorporation of [3h]leucine in the bacteria of 18 to 48 h-old colonies of pseudomonas aeruginosa, pseudomonas putida, bacillus thuringiensis, staphylococcus aureus and escherichia coli enabled the localization of bacterial multiplication sites by means of autoradiography of sagittal sections. in colonies where fast diameter expansion occurred, all the bacteria from the peripheral corona contributed to peripheral growth; in colonies where the expansion was slower, the growth rate of the bacteria ... | 1979 | 479831 |
| program of bacteriophage gh-1 dna transcription in infected pseudomonas putida. | the program of transcription in phage gh-1-infected pseudomonas putida was examined. it was found that the host p. putida rna polymerase transcribes early rna from the l strand of gh-1 dna during the initial stages of infection. the host rna polymerase is also undoubtedly responsible for transcription of complementary rna late in the infectious cycle because complementary rna was not transcribed when rifampin was added to the infected cell culture. the gh-1-induced rna polymerase transcribes lat ... | 1979 | 480467 |
| [dechlorination of 4-chlorophenol following extradiolic ring cleavage by pseudomonas putida]. | 1979 | 483865 | |
| the amino acid composition of histidine ammonialyase from pseudomonas putida ncib 10807. | the amino acid composition of histidine ammonia-lyase from pseudomonas putida ncib 10807 suggests that this enzyme may be different from the pseudomonas testosteroni ncib 10808 histidine ammonia-lyase, whose amino acid composition is known. | 1979 | 488259 |
| creatinine amidohydrolase (creatininase) from pseudomonas putida. purification and some properties. | 1979 | 500580 | |
| regulatory properties of histidase from pseudomonas putida. | 1979 | 518595 | |
| electron microscopy of some rock phosphate dissolving bacteria and fungi. | bacteria pseudomonas striata, bacillus polymyxa, b. megaterium and b. pulvifaciens, and fungi aspergillus awamori, a. niger and penicillium digitatum dissolve tricalcium phosphate and, much less, mussorie and udaipur rock phosphate. the solubilizing power of fungi was higher than that of bacteria, the highest being with a. awamori and a. niger, and with p. striata. electron microscopy of the various cultures showed an electron-dense layer on the bacterial surface after negative staining. the siz ... | 1979 | 527907 |
| local anesthetics block induction of the pseudomonas alk regulon. | the local anesthetics procaine and piperocaine blocked induction of the plasmid-determined enzymatic activities involved in the metabolism of n-alkanes in pseudomonas putida. procaine reversibly inhibited existing alkane hydroxylase activity. induction of a soluble aliphatic amidase activity was not affected. these results support the hypothesis that induction of the plasmid-determined alkane metabolic system in p. putida involves a membrane component(s). | 1979 | 533765 |
| regulation of membrane peptides by the pseudomonas plasmid alk regulon. | pseudomonas putida strains carrying the plasmid alk genes will grow on n-alkanes. induced alk+ strains contain membrane activities for alkane hydroxylation and dehydrogenation of aliphatic primary alcohols. p. putida cytoplasmic and outer membranes can be separated by sucrose gradient centrifugation after disruption of cells by either mild detergent lysis or passage through a french press. both the membrane component of alkane hydroxylase and membrane alcohol dehydrogenase fractionated with the ... | 1979 | 533768 |
| coexistence of different pathways in the metabolism of n-propylbenzene by pseudomonas sp. | pseudomonas desmolytica s449b1 and pseudomonas convexa s107b1 grown on n-propylbenzene oxidized n-propylbenzene to beta-phenylpropionic acid and benzoic acid by initial oxidation of the n-propyl side chain and the following beta-oxidation, respectively. the same strains also oxidized n-propylbenzene to 3-n-propylcatechol by initial oxidation of positions 2 and 3 of the aromatic nucleus. a ring fission product, 2-hydroxy-6-oxononanoic acid, was also isolated from the culture broth. together with ... | 1979 | 543699 |
| [purification and crystallization of a superoxide dismutase from pseudomonas putida]. | 1979 | 551636 | |
| lipoate metabolism in pseudomonas putida lp: thiolsulfinates of lipoate and bisnorlipoate. | 1978 | 343716 | |
| bacterial catabolism of lipoic acid. isolation and identification of a methyl ketone. | a soil bacterium, pseudomonas putida lp, can be grown on lipoate as sole source of carbon, sulfur, and energy. in addition to the previously identified catabolites (bisnorlipoate, tetranorlipoate, beta-hydroxybisnorlipoate, lipoate thiolsulfinate, and two bisnorlipoate thiolsulfinates) isolated from cultures of the organism grown on [1,6-14c[lipoate, a methyl ketone (1,2-dithiolane-3-butyl-3'-one) has now been isolated and identified. this catabolite was isolated by solvent extraction and hydrop ... | 1978 | 357321 |
| anabolic ornithine carbamoyltransferase of escherichia coli and catabolic ornithine carbamoyltransferase of pseudomonas putida. steady-state kinetic analysis. | the anabolic and catabolic ornithine carbamoyltransferases of pseudomonas putida display an undirectional catalytic specialization: in citrulline synthesis for the anabolic enzyme, in citrulline phosphorolysis for the catabolic one. the irreversibility of the anabolic enzyme in vitro has been previously explained by its kinetic properties, whereas the irreversibility of the catabolic transferase in vivo was shown to be due to its allosteric behaviour. in this work a steady-state kinetic analysis ... | 1978 | 359326 |
| microbiological transformations of terpenes: part xxv--enzymes involved in the degradation of linalool in the pseudomonas incognita, linalool strain. | 1978 | 367951 | |
| amino acid sequence of a peptide containing an essential cysteine residue of escherichia coli gmp synthetase. | the amino acid sequence of a 51-residue tryptic peptide of citraconylated [1-14c]carboxamidomethyl-labeled escherichia coli gmp synthetase was determined by sequenator analyses of the intact peptide and fragments obtained by cleavage of the peptide with cyanogen bromide, trypsin, and staphylcoccus aureus strain v8 protease. the cysteine residue of this peptide fragment is essential for glutamine-dependent gmp synthesis activity and is implicated in formation of a hypothetical covalent glutamyl-e ... | 1978 | 213434 |