Publications

[cloning and localization of the replication and mobilization regions in the d-plasmid of pseudomonas putida pbs286 (incp-9) with a broad host range].	rep-mob loci of naphthalene degradative plasmid pbs286 (incp-9) have been cloned on the escherichia coli vectors puc19 and pubr322. these loci confer to recombinant plasmids pbs952 and pbs953 the ability for effective mobilization by rp4 (incp-1) and f plasmid, as well as constant maintenance in various gram-negative bacteria. localization of cloned sequences in the restriction fragments of conservative part of the pbs286 genome was established. the data obtained correlate with the analysis of p ...	1988	2844624
nucleotide sequence of the regulatory gene xylr of the tol plasmid from pseudomonas putida.	we have determined the nucleotide sequence of the xylr gene for a transcriptional activator for the degradative pathway of aromatic hydrocarbons on the tol plasmid from pseudomonas putida. the 1698-bp sequence for a 566-amino acid (aa) protein (mr 63741) was identified as the xylr-encoding sequence. three regions in xylr show homology to klebsiella pneumoniae ntrc and nifa, both of which are transcriptional activators for the ntr and nif genes involved in the nitrogen metabolism. the central reg ...	1988	3169574
specificity of pyoverdine-mediated iron uptake among fluorescent pseudomonas strains.	pyoverdine-mediated iron transport was determined for seven fluorescent pseudomonas strains belonging to different species. for all strains, cell or cell outer membrane and iron(iii)-pyoverdine combinations were compared with their homologous counterparts in uptake, binding, and cross-feeding experiments. for four strains (pseudomonas putida atcc 12633, pseudomonas fluorescens w, p. fluorescens atcc 17400, and pseudomonas tolaasii ncppb 2192), the pyoverdine-mediated iron transport appeared to b ...	1988	3170485
[clinical study of cefuzonam in the field of obstetrics and gynecology].	cefuzonam (czon, l-105) was used clinically for the treatment of obstetrical and gynecological infections at a dosage of 1 g once or twice daily by intravenous drip infusion. the results obtained are summarized as follows. 1. clinical effects of czon were analyzed in 10 patients, including 5 patients with intrapelvic infections, 3 with intrauterine infections, and 1 each with adnexitis and an external genital infection. excellent responses were observed in 1 patient (11.1%), good responses in 7 ...	1988	3172464
[physical map of the dna of bacteriophage tf of pseudomonas putida].	a physical map has been constructed for p. putida bacteriophage tf dna containing single-strand breaks (nicks). localization of cleavage sites for ecori, hindiii, hpai clai, bamhi, sali, xbai and xhoi restriction endonucleases was determined. position of single-strand breaks was mapped by electrophoretic analysis of denatured tf dna and electron microscopy of partially denatured dna samples. the tf genome is characterized by the presence of two classes of nicks differing in the frequency of thei ...	1988	3173374
[purification and properties of two enzymes of meta-cleaving the aromatic ring controlled by the biphenyl biodegradation plasmid pbs 241 from pseudomonas putida].	it was shown that two metapyrocatechases (ec 1.13.11.2) function in pseudomonas putida bs893. biphenyl degradative plasmid pbs241 carries the genes of these enzymes. the basic properties of the both enzymes, i. e., mpc1 and mpc2, were investigated. it was found that mpc1 is an enzyme with a molecular mass of 135 kd and has a heterotetrameric subunit structure (alpha 2 beta 2), being made up of two non-identical polypeptides with mr of 34 and 22.5 kd; pi is 5.15, the ph optimum is at 8.0, a tempe ...	1988	3179349
[genes coding for rna-polymerase in bacteria. ii. conservative sites in the central region of the beta-subunit of pseudomonas putida rna-polymerase].	sali--l fragment of the p. putida rpobc operon has been sequenced and conservative regions of the central part of the rna-polymerase beta-subunit have been determined. amino and acid residues interacting with zn2+ are postulated.	1988	3190780
[isolation, purification and various properties of l-lysine-2-monooxygenase from pseudomonas putida].	isolation and purification of l-lysine-2- monooxygenase from the bacterium pseudomonas species was carried out. the purification procedure included ammonium sulfate fractionation, acid treatment, gel filtration through sephadex g-200 and ion-exchange chromatography on deae-sephadex a-50. such treatment resulted in more than 220-fold purification and 22% yield; the specific activity of the enzyme is 14.6 u/mg. the enzyme spectrum is typical for flavoproteins, with peaks at 275, 386 and 462 nm. at ...	1988	3191192
purification and properties of catechol 1,2-dioxygenase (pyrocatechase) from pseudomonas putida mt-2 in comparison with that from pseudomonas arvilla c-1.	catechol 1,2-dioxygenase (pyrocatechase) has been purified to homogeneity from pseudomonas putida mt-2. most properties of this enzyme, such as the absorption spectrum, iron content, ph stability, ph optimum, substrate specificity, km values, and amino acid composition, were similar to those of catechol 1,2-dioxygenase obtained from pseudomonas arvilla c-1 [y. kojima et al. (1967) j. biol. chem. 242, 3270-3278]. these two catechol 1,2-dioxygenases were also found, from the results of ouchterlony ...	1988	3214177
[genes coding for rna polymerase in bacteria. iii. the use of modified sanger's method for sequencing the c-terminal region of rpob gene, n-terminal region of rpoc gene and intercistron region of rna polymerase in pseudomonas putida].	the sanger method was modified and the primary structure of the sali-c fragment of the pseudomonas putida rpobc operon was elucidated.	1988	3219133
crystal structure determination, refinement and molecular model of creatine amidinohydrolase from pseudomonas putida.	the three-dimensional crystal structure of creatine amidinohydrolase (creatinase ec 3.5.3.3) from pseudomonas putida, a dimeric enzyme with a molecular weight of 97,000, has been determined by multiple isomorphous replacement, averaging over the local dyad and restrained crystallographic refinement at 1.9 a with a crystallographic r-value of 17.7%. the asymmetric unit contains a dimer. the two chemically identical subunits consist of 403 residues each. a subunit is built up of two domains, a sma ...	1988	3221393
4-methoxybenzoate monooxygenase from pseudomonas putida: isolation, biochemical properties, substrate specificity, and reaction mechanisms of the enzyme components.		1988	3226294
in vivo generation of r68.45-ppgh1 hybrid plasmids conferring a phl+ (meta pathway) phenotype.	plasmid ppgh1 originating from pseudomonas putida strain h carries all the genes required for the degradation of phenol (or cresols) via the meta cleavage pathway. besides mobilization of ppgh1 by a plasmid of the incompatibility group p-1, hybrid plasmids conferring the phl+ phenotype could be selected, when r68.45 was the conjugative plasmid. the hybrids contain the complete r68.45 and part of ppgh1. integration of phl-dna of ppgh1 into r68.45 occurred exclusively via the is21 region of r68.45 ...	1988	3226424
degradation of 1,4-naphthoquinones by pseudomonas putida.	pseudomonas putida j1 and j2, enriched from soil with juglone, are capable of a total degradation of 1,4-naphthoquinone, 2-hydroxy-1,4-naphthoquinone, and 2-chloro-1,4-naphthoquinone. naphthazerin and plumbagin are only converted into the hydroxyderivatives 2-hydroxynaphthazerin and 3-hydroxyplumbagin, respectively, whereas 2-amino-1,4-naphthoquinone is not attacked at all. the degradation of 1,4-naphthoquinone begins with a hydroxylation of the quinoid ring, yielding 2-hydroxy-1,4-naphthoquinon ...	1988	3228489
cloning, nucleotide sequence and characterization of genes encoding naphthalene dioxygenase of pseudomonas putida strain ncib9816.	we have cloned the naphthalene dioxygenase(nd)-coding genes from pseudomonas putida strain ncib9816 based on their ability to convert indole to indigo. the region coding for this enzyme activity was sequenced and three successive open reading frames were found. the corresponding gene products were identified using the t7 polymerase/promoter system. all of them are necessary for the nd activity. a comparison of the nd-coding genes with the ones coding for benzene dioxygenase revealed significant ...	1988	3243438
loss of the toluene-xylene catabolic genes of tol plasmid pww0 during growth of pseudomonas putida on benzoate is due to a selective growth advantage of 'cured' segregants.	during growth on benzoate-minimal medium pseudomonas putida mt-2 (paw1) segregates derivative ('cured') strains which have lost the ability to use the pathway encoded by its resident catabolic plasmid pww0. experiments with two plasmids identical to pww0 but each with an insert of tn401, which confers resistance to carbenicillin, suggested that the 'benzoate curing' occurs far more frequently by the specific deletion of the 39 kbp region carrying the catabolic genes than by total plasmid loss. t ...	1988	3246596
[a comparative study of the formation of 2-keto-d-gluconic acid by free and immobilized cells of pseudomonas putida].	the effect of glucose, oxygen and 2-keto-d-gluconic acid (2kg) concentrations on the 2kg production by free and immobilized cells of pseudomonas putida was studied. the effect of these factors was found to be similar in case of both free and immobilized cells, but the rate of the 2kg production by the free cells was a little higher as compared to the immobilized cells.	1988	3249741
[purification and properties of beta-ketoadipyl-coenzyme a thiolase from pseudomonas putida].		1988	3250096
comparison of the meta pathway operons on nah plasmid pww60-22 and tol plasmid pww53-4 and its evolutionary significance.	the regulated meta pathway operon for the catabolism of salicylate on the naphthalene plasmid pww60-22 was cloned into the broad-host-range vector pkt230 on a 17.5 kbp bamhi fragment. the recombinant plasmid conferred the ability to grow on salicylate when mobilized into plasmid-free pseudomonas putida paw130. a detailed restriction map of the insert was derived and the locations of some of the genes were determined by subcloning and assaying for their gene products in escherichia coli and p. pu ...	1988	3254935
in vitro and in vivo antibacterial activities of me1207, a new oral cephalosporin.	me1207 (pivaloyloxymethyl ester of me1206) is a new oral cephalosporin. me1206 is (6r,7r)-7-[(z)-2-(2-aminothiazol-4-yl)-2-(methoxyimino)- acetamido]-3-[(z)-2-(4-methylthiazol-5-yl)-ethyl]-cephem-4-carboxy lic acid. the susceptibilities of about 1,600 clinical isolates to me1206 were determined by the agar dilution method. me1206 showed a broad spectrum of activity against gram-positive and gram-negative bacteria. me1206 was more active than cefaclor, t-2525, and cefixime against staphylococcus ...	1988	3264132
purification and characterization of a bacterial nitrophenol oxygenase which converts ortho-nitrophenol to catechol and nitrite.	a nitrophenol oxygenase which stoichiometrically converted ortho-nitrophenol (onp) to catechol and nitrite was isolated from pseudomonas putida b2 and purified. the substrate specificity of the enzyme was broad and included several halogen- and alkyl-substituted onps. the oxygenase consisted of a single polypeptide chain with a molecular weight of 58,000 (determined by gel filtration) or 65,000 (determined on a sodium dodecyl sulfate-polyacrylamide gel). the enzymatic reaction was nadph dependen ...	1988	3350791
[products of biphenyl catabolism by a pseudomonas putida strain carrying the biodegradation plasmid pbs 241].		1988	3351100
trichloroethylene metabolism by microorganisms that degrade aromatic compounds.	trichloroethylene (tce) was metabolized by the natural microflora of three different environmental water samples when stimulated by the addition of either toluene or phenol. two different strains of pseudomonas putida that degrade toluene by a pathway containing a toluene dioxygenase also metabolized tce. a mutant of one of these strains lacking an active toluene dioxygenase could not degrade tce, but spontaneous revertants for toluene degradation also regained tce-degradative ability. the resul ...	1988	3355147
properties and functions of two succinic-semialdehyde dehydrogenases from pseudomonas putida.	two forms of succinic-semialdehyde dehydrogenase have been isolated in pseudomonas putida. the two enzymes could be separated by filtration on sephacryl s-300 and their apparent molecular weights were approx. 200,000 and 100,000. the smaller enzyme, which is induced by growth on 4-hydroxyphenylacetate, has been purified to 88% homogeneity by anion-exchange and affinity chromatography. electrophoresis in sodium dodecyl sulphate gave rise to a molecular weight of 53,000, indicating that the native ...	1988	3355840
primary structure of protein b from pseudomonas putida, member of a new class of 2fe-2s ferredoxins.	the primary structure of the 2fe-2s ferredoxin (protein b) from the benzene dioxygenase system of pseudomonas putida strain ncib 12190 was determined by gas-phase sequencing of the protein and its fragments. fast atom bombardment mass spectrometry indicated a molecular mass of 11,860 da. the sequence contained five cysteine residues, four of which would be required to coordinate the iron-sulphur cluster. the amino acid sequence determined in the present study is compared to that of a protein ded ...	1988	3360142
interaction of the protein components of 5-oxoprolinase. substrate-dependent enzyme complex formation.	5-oxo-l-prolinase from pseudomonas putida is composed of two reversibly dissociable proteins: component a catalyzes 5-oxoproline-dependent cleavage of atp, but does not catalyze the decyclization of 5-oxoproline; component b is required for the coupling of atp cleavage to ring-opening of 5-oxoproline to form glutamate (seddon, a. p., li, l., and meister, a. (1984) j. biol. chem. 259, 8091-8094). we describe here the purifications of components a and b to apparent homogeneity and the interactions ...	1988	3360791
bacterial metabolism of side chain fluorinated aromatics: cometabolism of 3-trifluoromethyl(tfm)-benzoate by pseudomonas putida (arvilla) mt-2 and rhodococcus rubropertinctus n657.	the tol plasmid-encoded enzymes of the methylbenzoate pathway in pseudomonas putida mt-2 cometabolized 3-trifluoromethyl (tfm)-benzoate. two products, 3-tfm-1,2-dihydroxy-2-hydrobenzoate (3-tfm-dhb) and 2-hydroxy-6-oxo-7,7,7-trifluoro-hepta-2,4-dienoate (7-tfhod) were identified chemically and by spectroscopic properties. tfm-substituted analogues of the metabolites of the methylbenzoate pathway were generally converted at drastically reduced rates. the catechol-2,3-dioxygenase from pseudomonas ...	1988	3365096
bacterial metabolism of side chain fluorinated aromatics: cometabolism of 4-trifluoromethyl(tfm)-benzoate by 4-isopropylbenzoate grown pseudomonas putida jt strains.	enzymes of the p-cymene pathway in pseudomonas putida strains cometabolized the intermediate analogue 4-trifluoromethyl(tfm)benzoate. three products, 4-tfm-2,3-dihydro-2,3-dihydroxybenzoate, 4-tfm-2,3-dihydroxybenzoate and 2-hydroxy-6-oxo-7,7,7-trifluorohepta-2,4-dienoate (7-tfhod) were identified chemically and by spectroscopic properties. certain tfm-substituted analogue metabolites of the p-cymene pathway were transformed at drastically reduced rates. hammett type analysis of ring cleavage re ...	1988	3365097
benzylic monooxygenation catalyzed by toluene dioxygenase from pseudomonas putida.	toluene dioxygenase, a multicomponent enzyme system known to oxidize mononuclear aromatic hydrocarbons to cis-dihydrodiols, oxidized indene and indan to 1-indenol and 1-indanol, respectively. in addition, the enzyme catalyzed dioxygen addition to the nonaromatic double bond of indene to form cis-1,2-indandiol. the oxygen atoms in 1-indenol and cis-1,2-indandiol were shown to be derived from molecular oxygen, whereas 70% of the oxygen in 1-indanol was derived from water. all of the isolated produ ...	1988	3365392
specific labeling of the essential cysteine residue of l-methionine gamma-lyase with a cofactor analogue, n-(bromoacetyl)pyridoxamine phosphate.	l-methionine gamma-lyase from pseudomonas putida is composed of four identical polypeptide chains and contains four cysteinyl residues per subunit. we have found one of them catalytically essential by its specific cyanylation with 2-nitro-5-thiocyanobenzoic acid. we have shown its essentiality also with n-(bromoacetyl)pyridoxamine 5'-phosphate (bapmp), which is a cofactor analogue and also an affinity-labeling agent. the kinetic data show that the apoenzyme forms a binary complex with bapmp prio ...	1988	3365412
cloning and characterization of a gene from rhizobium melilotii 2011 coding for ribosomal protein s1.	a 7 kb chromosomal dna fragment from r. melilotii was cloned, which complemented temperature-sensitivity of an e. coli amber mutant in rpsa, the gene for ribosomal protein s1 (es1). from complementation and maxicell analysis a 58 kd protein was identified as the homolog of protein s1 (rs1). dna sequence analysis of the r. melilotii rpsa gene identified a protein of 568 amino acids, which showed 47% identical amino acid homology to protein s1 from e. coli. the rs1 protein lacked the two cys resid ...	1988	3368316
transfer and expression of mesophilic plasmid-mediated degradative capacity in a psychrotrophic bacterium.	a psychrotrophic bacterium, originally isolated from a natural aquatic environment, was characterized and identified as pseudomonas putida q5 for use as a representative recipient for biodegradative genes from a mesophilic microorganism. the tol plasmid pwwo of the mesophile p. putida paw1 was successfully transferred by conjugation to the naturally isolated psychrotroph p. putida q5, as shown by plasmid analysis by agarose gel electrophoresis. expression of the genes encoded by the mesophilic t ...	1988	3377489
kinetics and mechanism of benzoylformate decarboxylase using 13c and solvent deuterium isotope effects on benzoylformate and benzoylformate analogues.	benzoylformate decarboxylase (benzoylformate carboxy-lyase, bfd; ec 4.1.1.7) from pseudomonas putida is a thiamine pyrophosphate (tpp) dependent enzyme which converts benzoylformate to benzaldehyde and carbon dioxide. the kinetics and mechanism of the benzoylformate decarboxylase reaction were studied by solvent deuterium and 13c kinetic isotope effects with benzoylformate and a series of substituted benzoylformates (pch3o, pch3, pcl, and mf). the reaction was found to have two partially rate-de ...	1988	3378056
difference between amino acid residues in the metal-ligand environments of iron- and manganese-superoxide dismutases.	alignment of the amino acid sequences of the pseudomonas ovalis and photobacterium leiognathi iron-superoxide dismutases (fe-sods) with the known sequences of the manganese-superoxide dismutases (mn-sods) shows that both types of sod are highly homologous (33-53% identity) and share residues for the metal coordination. the amino acid residues that form the environment of the metal ions appear to be also conserved between the fe- and mn-sods, except that the phe-84 and gln-154 in the mn-sods are ...	1988	3382418
purification and properties of 4-hydroxyphenylacetic acid 3-hydroxylase from pseudomonas putida.	4-hydroxyphenylacetic acid 3-hydroxylase is a key enzyme in the pathway for the microbial degradation of phenylalanine, tyrosine and many aromatic amines. this enzyme was purified to homogeneity from pseudomonas putida by affinity chromatography. the protein had a molecular weight of 91,000 and was a dimer of identical subunits. it was a typical external flavoprotein monooxygenase and showed an absolute requirement of nadh for activity. the enzyme had a ph optimum of 7.5 and the km values for 4- ...	1988	3401220
oxidation of substituted phenols by pseudomonas putida f1 and pseudomonas sp. strain js6.	the biodegradation of benzene, toluene, and chlorobenzenes by pseudomonas putida involves the initial conversion of the parent molecules to cis-dihydrodiols by dioxygenase enzyme systems. the cis-dihydrodiols are then converted to the corresponding catechols by dihydrodiol dehydrogenase enzymes. pseudomonas sp. strain js6 uses a similar system for growth on toluene or dichlorobenzenes. we tested the wild-type organisms and a series of mutants for their ability to transform substituted phenols af ...	1988	3415220
degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with pseudomonas putida f1.	toluene-induced cells of pseudomonas putida f1 removed trichloroethylene from growth media at a significantly greater initial rate than the methanotroph methylosinus trichosporium ob3b. with toluene-induced p. putida f1, the initial degradation rate varied linearly with trichloroethylene concentration over the range of 8 to 80 microm (1.05 to 10.5 ppm). at 80 microm (10.5 ppm) trichloroethylene and 30 degrees c, the initial rate was 1.8 nmol/min per mg of total cell protein, but the rate decreas ...	1988	3415234
similarity of the e1 subunits of branched-chain-oxoacid dehydrogenase from pseudomonas putida to the corresponding subunits of mammalian branched-chain-oxoacid and pyruvate dehydrogenases.	the genes encoding proteins responsible for activity of the e1 component of branched-chain-oxoacid dehydrogenase of pseudomonas putida have been subcloned and the nucleotide sequence of this region determined. open reading frames encoding e1 alpha (bkda1, 1233 bp) and e1 beta (bkda2, 1020 bp) were identified with the aid of the n-terminal sequence of the purified subunits. the mr of e1 alpha was 45,158 and of e1 beta was 37,007, both calculated without n-terminal methionine. the deduced amino ac ...	1988	3416875
molecular studies on the role of a root surface agglutinin in adherence and colonization by pseudomonas putida.	pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. mutants of p. putida derived chemically or by tn5 insertion demonstrated enhanced or decreased agglutinability. two nonagglutinable tn5 mutants (agg) and two mutants with enhanced agglutinability (agg) possessed tn5 in unique restriction sites. agg mutants colonized root surfaces of seedlings grown from inoculated seeds, but at levels lower than those observed with the agg parent. in short ...	1988	16347550
fusarium wilt suppression and agglutinability of pseudomonas putida.	mutants of pseudomonas putida (agg) that lack the ability to agglutinate with components present in washes of bean and cucumber roots showed limited potential to protect cucumber plants against fusarium oxysporum f. sp. cucumerinum. however, a higher level of protection was observed against fusarium wilt in cucumber plants coinoculated with the parental bacterium (agg), which was agglutinable. the agg mutants did not colonize the roots of cucumber plants as extensively as the agg parental isolat ...	1988	16347713
tn5-mediated cloning of a genetic region from pseudomonas putida involved in the stimulation of plant root elongation.	transposon (tn5) mutagenesis was applied to pseudomonas putida gr12-2r3, which promotes root elongation (a phenotype designated pre) of brassica campestris under gnotobiotic conditions. of 3,000 tn5 transconjugants, only one mutant that lost pre activity but remained prototrophic and capable of plant root colonization was detected. this mutant was complemented by plasmid pre53, which contained a 15.0-kilobase dna insert isolated from a parental strain. the complemented mutant regained full pre a ...	1988	16347807
thiosulfate oxidation by obligately heterotrophic bacteria.	thiosulfate was oxidized stoichiometrically to tetrathionate during growth on glucose byklebsiella aerogenes, bacillus globigii, b. megaterium, pseudomonas putida, two strains each ofp. fluorescens andp. aeruginosa, and anaeromonas sp. a gram-negative, rod-shaped soil isolate, pseudomonad hw, converted thiosulfate to tetrathionate during growth on acetate. none of the organisms could use thiosulfate as sole energy source. the quantitative recovery of all the thiosulfate supplied to heterotrophic ...	1988	24202996
the effect of pseudomonas putida colonization on root surface peroxidase.	increased activities of peroxidase and indole 3-acetic acid (iaa) oxidase were detected on root surfaces of bean (phaseolus vulgaris) seedlings colonized with a soil saprophytic bacterium, pseudomonas putida. iaa oxidase activity increased over 250-fold and peroxidase 8-fold. enhancement was greater for 6-day-old than for 4- or 8-day-old inoculated plants no iaa oxidase or peroxidase activities were associated with the bacterial cells. native polyacrylamide gel electrophoresis demonstrated that ...	1987	16665732
toluene induction and uptake kinetics and their inclusion in the specific-affinity relationship for describing rates of hydrocarbon metabolism.	the kinetics of concentration-dependent toluene metabolism were examined by evaluating each term in the second-order rate equation. marine and freshwater pseudomonads were used. uptake for pseudomonas sp. strain t2 was characterized by a completely saturatable system with small transport constant (k(t) = 44 mug/liter) and large specific affinity. kinetics for pseudomonas putida ppf1 were similar. induction had little effect on k(t), but it caused the specific affinity to increase from about 0.03 ...	1987	16347440
construction of chlorobenzene-utilizing recombinants by progenitive manifestation of a rare event.	separate continuous cultures of pseudomonas putida r5-3, grown on toluene, and pseudomonas alcaligenes c-o, grown on benzoate, were concentrated and continuously amalgamated on a ceramic bead column, which was subjected to a continuous stream of chlorobenzene vapors. a recombinant strain, p. putida cb1-9, was isolated in less than 1 month. p. alcaligenes c-0 grew on benzoate and 3-chlorobenzoate but not on toluene, p. putida r5-3 grew on benzoate and toluene but not on 3-chlorobenzoate, and neit ...	1987	3426217
laboratory and clinical evaluation of isolation media for campylobacter jejuni.	six selective isolation media were evaluated for their ability to support the growth of campylobacter jejuni. colony counts of 70 isolated strains of c. jejuni and recovery studies on these strains in simulated positive feces samples demonstrated that bolton and hutchinson' charcoal, cefoperazone, deoxycholate agar and karmali's charcoal-based selective medium produced the highest recovery rates with the greatest suppression of other fecal flora. c. jejuni colonies were more easily recognized on ...	1987	3429621
organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation.	three critical enzymes catechol oxygenase ii (chlorocatechol dioxygenase), muconate cycloisomerase ii, and dienelactone hydrolase, are involved in the degradation of chlorocatechols, which are obligatory intermediates in the catabolism of chlorinated aromatic compounds. the organization and complete nucleotide sequence of the genes for these enzymes have been determined on a 4.2-kilobase-pair (kbp) bgl ii fragment cloned from the plasmid pac27, based on the agreement of open reading frame length ...	1987	3299368
maintenance and stability of introduced genotypes in groundwater aquifer material.	three indigenous groundwater bacterial strains and pseudomonas putida harboring plasmids tol (pwwo) and rk2 were introduced into experimentally contaminated groundwater aquifer microcosms. maintenance of the introduced genotypes was measured over time by colony hybridization with gene probes of various specificity. on the basis of the results of colony hybridization quantitation of the introduced organisms and genes, all introduced genotypes were stably maintained at approximately 10(5) positive ...	1987	3300546
activation of the xyldlegf promoter of the tol toluene-xylene degradation pathway by overproduction of the xyls regulatory gene product.	the xyls regulatory gene of the pseudomonas putida tol plasmid (pwwo) has been cloned under the transcriptional control of the escherichia coli tac promoter in a broad-host-range controlled-expression vector. induction with isopropylthiogalactoside allowed overproduction and characterization of the xyls product by specific interaction with the tol meta-cleavage pathway operator-promoter region (op2) in vivo in e. coli. examination of plasmid-specified polypeptides in e. coli maxicells led to ide ...	1987	3301806
purification and properties of formylglutamate amidohydrolase from pseudomonas putida.	formylglutamate amidohydrolase (fgase) catalyzes the terminal reaction in the five-step pathway for histidine utilization in pseudomonas putida. by this action, n-formyl-l-glutamate (fg) is hydrolyzed to produce l-glutamate plus formate. urocanate, the first product in the pathway, induced all five enzymes, but fg was able to induce fgase alone, although less efficiently than urocanate did. this induction by fg resulted in the formation of an fgase with electrophoretic mobility identical to that ...	1987	3308850
molecular analysis of regulatory and structural xyl genes of the tol plasmid pww53-4.	pww53-4 is a cointegrate between rp4 and the catabolic plasmid pww53 from pseudomonas putida mt53, which contains 36 kbp of pww53 dna inserted close to the oriv gene of rp4; it encodes the ability to grow on toluene and the xylenes, characteristic of pww53, as well as resistance to tetracycline, kanamycin and carbenicillin, characteristic of rp4. a physical map of the 36 kbp insert of pww53 dna for 11 restriction enzymes is presented, showing that the relative positions of the two xyl operons ar ...	1987	3309179
effect of temperature on the stability of plasmid ptg201 and productivity of xyle gene product in recombinant escherichia coli: development of a two-stage chemostat with free and immobilized cells.	the effect of temperature on the stability of ptg201, a plasmid carrying the xyle gene (which encodes catechol 2,3-dioxygenase from pseudomonas putida), and the production of catechol 2,3-dioxygenase in free and immobilized escherichia coli during continuous culture have been studied at various temperatures. immobilization of cells increased the stability of ptg201 considerably, even under conditions when expression of the xyle product was enhanced. since xyle transcription was controlled by the ...	1987	3312486
[molecular genetic organization and origin of plasmid pbs52 with a broad range of bacterial hosts].	the data are presented on the localization of genetic determinants of resistance to streptomycin, ampicillin and sulfanilamides on the physical map of conjugative r plasmid pbs52 of 38,000 bp which has a broad bacterial host range and belongs to a new incompatibility group. the plasmid has a natural "polylinker" site (less than 200 bp) containing (in the order of arrangement) the recognition sites for restriction enzymes: bamhi-ecori-psti-ecorv-bglii (pvuii). the comparative analysis shows that ...	1987	3319772
qualitative evidence for expression of klebsiella pneumoniae nif in pseudomonas putida.	pseudomonas putida mt20-3 carrying the klebsiella pneumoniae nif plasmids prd1 or pmf250 showed highly o2-sensitive aerobic acetylene reduction on low-n pyruvate or glucose agar. this finding confirms unequivocally that k. pneumoniae nif can be expressed in an obligate aerobe.	1987	3329216
molecular cloning of genes encoding branched-chain keto acid dehydrogenase of pseudomonas putida.	we cloned the structural genes for the individual subunits of the branched-chain keto acid dehydrogenase multienzyme complex on a 7.8-kilobase ecori-ssti restriction fragment of pseudomonas putida chromosomal dna by cloning into the broad-host-range vector pkt230. a direct selection system for growth on valine-isoleucine agar was achieved by complementation of p. putida branched-chain keto acid dehydrogenase mutants. the recombinant plasmid, pss1-1, increased expression of branched-chain keto ac ...	1987	3549697
characterization of a novel tol-like plasmid from pseudomonas putida involved in 1,2,4-trimethylbenzene degradation.	a strain of pseudomonas putida (tmb) was found to resemble p. putida mt-2 (paw1) in its ability to degrade 1,2,4-trimethylbenzene, toluene, m-xylene, and p-xylene via oxidation of a methyl substituent and reaction of the meta fission pathway, but a different regulatory model is suggested. the ability of p. putida tmb to degrade these substrates was encoded by plasmid pgb (85 kilobase pairs), which showed considerable differences in size, restriction patterns, and dna sequence from those of plasm ...	1987	3558324
biosynthesis of thiamin. different biosynthetic routes of the thiazole moiety of thiamin in aerobic organisms and anaerobic organisms.	the nitrogen atom of glycine was incorporated into the thiazole moiety of thiamin in the aerobic microorganisms bacillus subtilis, pseudomonas putida, saccharomyces cerevisiae, mucor racemosus, neurospora crassa, and emericella nidulans. it was not incorporated in the case of the facultative anaerobic microorganisms escherichia coli and enterobacter aerogenes, which, however, did incorporate the nitrogen atom of tyrosine. these results show that aerobic microorganisms and facultative anaerobic m ...	1987	3566774
[effect of the medium composition on the accumulation of 2-keto-d-gluconic acid in pseudomonas putida cultures].	the effect of the composition of the culture medium and the age of the culture on the activities of the enzymes involved in accumulation of 2-ketogluconic acid by pseudomonas putida was studied. the activities of glucose and gluconate dehydrogenases that are responsible for direct oxidation of glucose to 2-ketogluconic acid, were 2-3 times higher during the active growth of the culture than in the stationary phase. the activities of 2-ketogluconokinase and 2-keto-6-phosphogluconate reductase, en ...	1987	3575266
survival of selected bacterial species in sterilized activated carbon filters and biological activated carbon filters.	the survival of selected hygienically relevant bacterial species in activated carbon (ac) filters on a bench scale was investigated. the results revealed that after inoculation of the test strains the previously sterilized ac absorbed all bacteria (10(6) to 10(7)). after a period of 6 to 13 days without countable bacteria in the effluent, the numbers of escherichia coli, pseudomonas aeruginosa, and pseudomonas putida increased up to 10(4) to 10(5) cfu/ml of effluent and 10(6) to 10(7) cfu/g of a ...	1987	3579281
recruitment of naphthalene dissimilatory enzymes for the oxidation of 1,4-dichloronaphthalene to 3,6-dichlorosalicylate, a precursor for the herbicide dicamba.	pseudomonas putida expresses plasmid-encoded enzymes and regulatory proteins for the dissimilation of naphthalene through salicylate and the alpha-keto acid pathway. a strain of p. putida (nah:tn5/g67) defective in salicylate hydroxylase (nahg) was assessed for its ability to oxidize 1,4-dichloronaphthalene. washed cell suspensions were shown to accumulate 3,6-dichlorosalicylate, which, after further chemical treatment, yields the herbicide dicamba (3,6-dichloro-2-methoxybenzoate). however, the ...	1987	3584076
expression of degradative genes of pseudomonas putida in caulobacter crescentus.	the recombinant plasmid rp4-tol was transferred into caulobacter crescentus at a high frequency, and the plasmid was maintained for at least 50 generations. c. crescentus cells which contained rp4-tol grew on all the aromatic compounds that the plasmid normally allowed pseudomonas putida to grow on. reciprocal transfers from c. crescentus donor to p. putida or escherichia coli recipients were less efficient and occurred at frequencies of approximately 10(-3). some representative tol-specified en ...	1987	3597317
comparison of a gelation and a chromogenic limulus (lal) assay for the detection of gram-negative bacteria, and the application of the latter assay to milk.	when a chromogenic limulus amoebocyte lysate (lal) assay and a tube gelation lal assay were compared for the detection of gram-negative bacteria using a strain of pseudomonas putida, the detection level (approximately 10(3) cfu/ml) and cost of the assays were approximately the same for both assays but the reading was more precise for the chromogenic substrate assay. a modified chromogenic assay was devised for detection of ps. putida in milk.	1987	3597923
pseudomonas putida. newly recognized pathogen in patients with cancer.	pseudomonas putida was recovered from blood culture specimens between 1980 and 1985 in 15 patients with cancer. no isolates were found in specimens obtained before 1980. eight patients were considered to have septicemia (more than one positive blood culture result plus clinical signs of infection). septicemia was monomicrobial in three of those eight patients and polymicrobial in five. of these eight patients, one had pneumonia and three had phlebitis, cellulitis, or both at the site of the veno ...	1987	3605136
cloning and complete nucleotide sequence determination of the catb gene encoding cis,cis-muconate lactonizing enzyme.	the enzyme, cis,cis-muconate lactonizing enzyme i (mlei; ec 5.5.1.1), has been proposed to play a key role in the beta-ketoadipate pathway of benzoate degradation. a 10.2-kb ecori fragment isolated from a pseudomonas putida genomic library complemented a mutant deficient in this enzyme. the mlei coding gene, catb, was localized to a 1.6-kb fragment which was sequenced by the dideoxy chain termination method. mlei was purified 25-fold from crude extracts of benzoate-grown p. putida prs2015 harbor ...	1987	3609743
overproduction of the xyls gene product and activation of the xyldlegf operon on the tol plasmid.	the effect of high-level expression of the regulatory gene xyls of the pseudomonas putida tol plasmid on the activation of the xyldlegf operon was investigated in escherichia coli. the xyls gene was placed downstream from the tac promoter, and the resultant fusion was cloned in cis to the xyldlegf operon. the expression of the operon was monitored by the level of catechol 2,3-dioxygenase, whose structural gene xyle was placed directly after the operator-promoter region of xyldlegf. xyls transcri ...	1987	3611023
18o studies on the 5-oxoprolinase reaction. evidence for a phosphorylated tetrahedral intermediate.	5-oxoprolinase catalyzes a reaction in which the cleavage of atp to adp and pi and the decyclization of 5-oxoproline to form glutamate are coupled. when the reaction catalyzed by 5-oxoprolinase of pseudomonas putida was carried out to 90% completion in h2(18)o, the residual 5-oxoproline was found to contain 18o in the amide carbonyl oxygen atom. such isotopic incorporation was not observed in similar studies with a subunit of the enzyme which catalyzes 5-oxoproline-dependent atpase and formation ...	1987	3611103
nucleotide sequence and expression of gene nahh of plasmid nah7 and homology with gene xyle of tol pwwo.	the enzyme catechol 2,3-dioxygenase (c23o) encoded by the nahh gene of plasmid nah7 converts catechol to alpha-hydroxymuconic epsilon-semialdehyde in pseudomonas putida. we have cloned this structural gene into vectors puc18 and pkt240, determined the nucleotide sequence and deduced the amino acid sequence. in comparison to the gene xyle of the tol plasmid pwwo which encodes a similar c230 enzyme [nakai et al. j. biol. chem. (1983b), 2923-2928], the respective g + c contents were 55% and 57%, th ...	1987	3623105
use of salicylate to estimate the "threshold" inducer level for de novo synthesis of the phenol-degrading enzymes in pseudomonas putida strain h.	a special approach was used to elucidate the "threshold" inducer concentration for coordinative de novo synthesis of phenol hydroxylase(s), catechol 2,3-dioxygenase and the 2-hydroxymuconic semialdehyde-metabolizing enzymes which initiate phenol catabolism in pseudomonas putida strain h. it is based on cell-precultivation with glucose (as the carbon and energy source) in the presence of different concentrations of sodium salicylate which proved to be a potent non-metabolizable inducer in strain ...	1987	3656095
high-resolution crystal structure of cytochrome p450cam.	the crystal structure of pseudomonas putida cytochrome p450cam with its substrate, camphor, bound has been refined to r = 0.19 at a normal resolution of 1.63 a. while the 1.63 a model confirms our initial analysis based on the 2.6 a model, the higher resolution structure has revealed important new details. these include a more precise assignment of sequence to secondary structure, the identification of three cis-proline residues, and a more detailed picture of substrate-protein interactions. in ...	1987	3656428
l-methionine gamma-lyase from pseudomonas putida and aeromonas.		1987	3657560
amino acid sequence of iron-superoxide dismutase from pseudomonas ovalis.	the amino acid sequence of iron-superoxide dismutase from pseudomonas ovalis was deduced by the analyses of peptides derived from limited hydrolysis of the aminoethylated or pyridylethylated apoprotein with trypsin, staphylococcus aureus v8 protease, and dilute acid hydrolysis. the polypeptide chain contains 195 amino acid residues and has a calculated mr of 21,421. the sequence is highly homologous (65% identity) to the recently published sequence of the iron-superoxide dismutase from photobact ...	1987	3666146
nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of pseudomonas putida.	the nucleotide sequence of the genes from pseudomonas putida encoding oxidation of benzene to catechol was determined. five open reading frames were found in the sequence. four corresponding protein molecules were detected by a dna-directed in vitro translation system. escherichia coli cells containing the fragment with the four open reading frames transformed benzene to cis-benzene glycol, which is an intermediate of the oxidation of benzene to catechol. the relation between the product of each ...	1987	3667527
the effect of lipophilic weak acids on the segregational stability of tol plasmids in pseudomonas putida.	the effect of various lipophilic weak acids on the stability of certain tol plasmids was investigated. benzoate induced deletion of tol plasmid dna in pseudomonas putida mt15, followed by loss of the plasmid; this effect was ph- and concentration-dependent, suggesting that undissociated benzoic acid was a more effective curing agent than the benzoate anion. plasmid loss always approached a frequency of 100% after a lag and apparently depended on the prior occurrence of deletions, although delete ...	1987	3668501
dienelactone hydrolase from pseudomonas sp. strain b13.	dienelactone hydrolase (ec 3.1.1.45) catalyzes the conversion of cis- or trans-4-carboxymethylenebut-2-en-4-olide (dienelactone) to maleylacetate. an approximately 24-fold purification from extracts of 3-chlorobenzoate-grown pseudomonas sp. strain b13 yielded a homogeneous preparation of the enzyme. the purified enzyme crystallized readily and proved to be a monomer with a molecular weight of about 30,000. each dienelactone hydrolase molecule contains two cysteinyl side chains. one of these was ...	1987	3804973
nucleotide sequence and expression of clcd, a plasmid-borne dienelactone hydrolase gene from pseudomonas sp. strain b13.	the clcd structural gene encodes dienelactone hydrolase (ec 3.1.1.45), an enzyme that catalyzes the conversion of dienelactones to maleylacetate. the gene is part of the clc gene cluster involved in the utilization of chlorocatechol and is carried on a 4.3-kilobase-pair bglii fragment subcloned from the pseudomonas degradative plasmid pac27. a 1.9-kilobase-pair psti-ecori segment subcloned from the bglii fragment was shown to carry the clcd gene, which was expressed inducibly under the tac promo ...	1987	3804974
amidohydrolysis of n-methylhydantoin coupled with atp hydrolysis.	a new enzyme, n-methylhydantoin amidohydrolase, was highly purified from pseudomonas putida 77: it catalyzes the hydrolysis of n-methylhydantoin to n-carbamoylsarcosine with the concomitant stoichiometric cleavage of atp to adp and orthophosphate. the enzyme absolutely requires atp, mg2+ and k+ for the n-methylhydantoin hydrolysis. the rapid and complete degradation of n-methylhydantoin during the cultivation of p. putida 77, which rapidly degrades creatinine via only n-methylhydantoin and which ...	1987	3827889
hydrodynamic characterization of the size and shape of atropinesterase from pseudomonas putida.	atropinesterase from pseudomonas putida has been investigated by means of different ultracentrifugation methods under native and denaturing conditions. the following quantities were determined: sedimentation coefficient, translational diffusion and friction coefficient, partial specific volume and molecular weight. from these data the size, shape and hydration of the enzyme molecule in solution were estimated. the results suggest that atropinesterase is a globular protein which consists of a sin ...	1987	3828356
benzene dioxygenase in pseudomonas putida. subunit composition and immuno-cross-reactivity with other aromatic dioxygenases.	the terminal oxygenase component of benzene dioxygenase from pseudomonas putida strain ml2 was shown to contain two subunits, of mr 54,500 and 23,500, by sds/polyacrylamide-gel electrophoresis. the native mr of the terminal oxygenase was estimated to be 168,000 +/- 4000. polyclonal antibodies raised against each of the subunits cross-reacted with two polypeptides in cell-free extracts from toluene-grown pseudomonas putida strain n.c.i.b. 11767. the mr values of these polypeptides were similar to ...	1987	3446181
bacterial flora in bottled uncarbonated mineral drinking water.	a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...	1987	3446349
regulatory circuits controlling transcription of tol plasmid operon encoding meta-cleavage pathway for degradation of alkylbenzoates by pseudomonas.	tol plasmid pwwo of pseudomonas putida contains two operons that specify a pathway for the degradation of aromatic hydrocarbons. the 'upper' operon encodes enzymes for the oxidation of toluene to benzoate and xylenes to toluates, whereas the meta-cleavage operon specifies the further oxidation of benzoate and toluates. transcription of the upper pathway operon is positively regulated by the xylr protein, which is activated by toluene/xylenes and their alcohol catabolic products, in combination w ...	1987	3448461
the xyls gene positive regulator of tol plasmid pwwo: identification, sequence analysis and overproduction leading to constitutive expression of meta cleavage operon.	the pseudomonas putida tol plasmid pwwo carries an operon that specifies a meta-cleavage pathway for the catabolism of benzoate and toluates whose transcription is positively regulated by the xyls gene product. stimulation of transcription of the operon is thought to result from activation of this protein by pathway substrates/effectors. in the present study, overexpression of the xyls gene has led to identification of the regulator as a 33 kda protein. overexpression of xyls also resulted in pa ...	1987	3475526
structure of the pseudomonas putida alkbac operon. identification of transcription and translation products.	the structural genes of the pseudomonas oleovorans alk (alkane utilization) system, which are localized on the alkbac operon, were cloned as a 16.9-kilobase pair ecori fragment. we have measured the length and determined the position of the alkbac operon on this fragment by electron microscopy of r-loops. furthermore, the 7.3-kilobase pair long alkbac operon was analyzed for translation products in escherichia coli minicells. using a spectrum of overlapping subclones, six different proteins were ...	1987	3032966
construction of a transposon containing a gene for polygalacturonate trans-eliminase from klebsiella oxytoca.	a dna fragment containing a klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon tn5. this new transposon, designated tn5-pga+, had a transposition frequency of 1 x 10(-6). the broad host range plasmid pr751::tn5-pga+ was conjugally transferred to a variety of genetic backgrounds. the ability to degrade polygalacturonate was expressed in aeromonas hydrophila, alcaligenes eutrophus, azotomonas insolita, escherichia coli, pseudomonas put ...	1987	3034186
interposon mutagenesis of soil and water bacteria: a family of dna fragments designed for in vitro insertional mutagenesis of gram-negative bacteria.	we have constructed a series of derivatives of the omega interposon [prentki and krisch, gene 29 (1984) 303-313] that can be used for in vitro insertional mutagenesis. each of these dna fragments carries a different antibiotic or hg2+ resistance gene (apr, cmr, tcr, kmr or hgr) which is flanked, in inverted orientation, by transcription and translation termination signals and by synthetic polylinkers. the dna of these interposons can be easily purified and then inserted, by in vitro ligation, in ...	1987	3038679
discovery of a cutinase-producing pseudomonas sp. cohabiting with an apparently nitrogen-fixing corynebacterium sp. in the phyllosphere.	a phyllospheric bacterial culture, previously reported to partially replace nitrogen fertilizer (b. r. patti and a. k. chandra, plant soil 61:419-427, 1981) was found to contain a fluorescent pseudomonas which was identified as pseudomonas putida and a corynebacterium sp. the p. putida isolate was found to produce an extracellular cutinase when grown in a medium containing cutin, the polyester structural component of plant cuticle. the corynebacterium sp. grew on nitrogen-free medium but could n ...	1987	3793714
purification and characterization of adhesive exopolysaccharides from pseudomonas putida and pseudomonas fluorescens.	in this study, the adhesive exopolysaccharides of strains of pseudomonas putida and p. fluorescens, both isolated from freshwater epilithic communities, were examined with regard to their chemical composition, biosynthesis, and their role in adhesion. electron microscopy showed that both strains were enrobed in fibrous glycocalyces and that these structures were involved in attachment of the cells to a solid surface and as structural matrices in the microcolony mode of growth. in batch culture e ...	1987	2451553
phosphate-selective porins from the outer membranes of fluorescent pseudomonas sp.	phosphate starvation induced oligomeric proteins from the outer membranes of pseudomonas fluorescens, pseudomonas putida, pseudomonas aureofaciens, and pseudomonas chlororaphis were purified to homogeneity. the incorporation of the purified proteins into planar lipid bilayer membranes resulted in stepwise increases in membrane conductance. single channel conductance experiments demonstrated that these proteins were all capable of forming small channels, similar to the pseudomonas aeruginosa phos ...	1987	2436733
expression of the regulatory gene xyls on the tol plasmid is positively controlled by the xylr gene product.	the regulatory gene xyls on the tol plasmid of pseudomonas putida activates the transcription of the xyldlegf operon for the m-toluate-degrading pathway in the presence of m-toluate. the gene also activates the transcription of the same operon in the presence of m-xylene or m-methylbenzyl alcohol, but for this activation another regulatory gene, xylr, is required. in this study we examined the xyls expression by determining the mrna by reverse transcriptase mapping and by monitoring the enzyme a ...	1987	2440045
analysis of transcription from the trfa promoter of broad host range plasmid rk2 in escherichia coli, pseudomonas putida, and pseudomonas aeruginosa.	reverse transcriptase mapping has been used to analyze transcription from the trfa promoter of broad host range plasmid rk2. the results show that trfa operon mrna has the same 5' end in pseudomonas aeruginosa, pseudomonas putida, and escherichia coli. the strengths of wild-type and mutant trfa promoters, which differ by defined base substitutions, have been compared and the positions of their transcriptional start sites determined. while these base substitutions do not alter the transcriptional ...	1987	2442786
effect of temperature on sulfite-mediated dark reversion of urocanase in pseudomonas putida.		1987	2888143
gene organization of the first catabolic operon of tol plasmid pww53: production of indigo by the xyla gene product.	the entire operon coding for the enzymes responsible for conversion of toluenes to benzoates has been cloned from tol plasmid pww53 and the position of the genes accurately located. the coding region was 7.4 kilobase pairs (kbp) long, and the gene order was operator-promoter region (op1)-a small open reading frame-xylc (1.6 kbp)-xyla (2.9 kbp)-xylb (1.8 kbp). within the coding region there was considerable homology with the isofunctional region of the archetypal tol plasmid pww0. a central regio ...	1987	3027047
the amino acid sequence of the aliphatic amidase from pseudomonas aeruginosa.	amino acid sequence studies show that the aliphatic amidase (ec 3.5.1.4) from pseudomonas aeruginosa pac142 consists of a single polypeptide chain of 346 residues, giving an mr of 38,400. the evidence from the amino acid studies is in complete agreement with that deduced from the dna sequence of the amie gene. studies of the protein from pseudomonas putida a87 show that it differs from the ps. aeruginosa protein by about 30 amino acid substitutions. it now becomes possible to relate changes in t ...	1987	3108029
genetic analysis of mannityl opine catabolism in octopine-type agrobacterium tumefaciens strain 15955.	the genetic organization of functions responsible for mannityl opine catabolism of the ti plasmid of agrobacterium tumefaciens strain 1,5955 was investigated. a partial hindiii digest of pti1,5955 was cloned into a broad host range cosmid and the clones obtained were tested for ability to confer mannityl opine degradation upon agrobacterium. inserts containing genes for catabolism of mannopinic acid, mannopine, agropine, and agropinic acid were obtained, spanning a segment of 43 kb on the ti pla ...	1987	3112522
[automated micromethod for the determination of the utilization of carbon sources by clinically significant pseudomonas species].	the assimilation of 43 different carbon substrates by 93 clinical strains of pseudomonas aeruginosa was studied by a new miniaturized rapid method. reading of assimilation results was done photometrically after 18-20 h incubation and the resulting data were captured and stored by a microcomputer. the differentiating capacity of the assimilation tests were verified by comparing the results of 41 strains of pseudomonas fluorescens, 48 strains of pseudomonas putida, 52 strains of pseudomonas maltop ...	1987	3118596
[expression of the human interferon alpha f gene in the obligate methylotroph methylobacillus flagellatum kt and pseudomonas putida].	the expression of human leucocyte interferon alpha f gene in plasmid plm-ifn alpha f-273 is controlled by a hybrid tac (trp-lac) promoter. a structural gene for interferon alpha f is a component of the hybrid operon lacz'-ifn alpha f-tcr, that contains an e. coli trp-operon intercystronic region. plasmid plm ifn alpha f-273--directed interferon synthesis allows to obtain about 10(7) iu/l. this plasmid was cloned in broad-host-range vector plasmid payc31. the hybrid bi-repliconed plasmid containi ...	1987	3119998
a set of cassettes and improved vectors for genetic and biochemical characterization of pseudomonas genes.	a set of broad-host-range vectors allowing direct selection of recombinant dna molecules to facilitate subcloning and expression analyses of pseudomonas genes was constructed using bg/ii lacz alpha cassette. controlled expression vectors pvdtac39 and pvdtac24 were shown to be useful for determination of enzymatic activities encoded by the cloned dna fragments and mr determination of the corresponding polypeptides. a set of pseudomonas putida xyle gene cassettes truncated at the 5' end was constr ...	1987	3123327
molecular cloning of the pseudomonas putida glyoxalase i gene in escherichia coli.	the glyoxalase i gene of pseudomonas putida was cloned onto a vector plasmid pbr 322 as a 7.5 kilobase sau 3ai fragment of chromosomal dna and the hybrid plasmid was designated pgi 318. the gene responsible for the glyoxalase i activity in pgi 318 was recloned in pbr 322 as a 2.2 kilobase hin diii fragment and was designated pgi 423. the p. putida glyoxalase i gene on pgi 318 and pgi 423 was highly expressed in e. coli cells and the glyoxalase i activity level was increased more than 150 fold in ...	1987	2820418
isolation and characterization of pseudomonas putida r-prime plasmids.	a number of enhanced chromosome mobilizing (ecm) plasmids derived from the wide host range plasmid r68 have been used to construct r-prime plasmids carrying a maximum of two map minutes of the pseudomonas putida ppn chromosome, using pseudomonas aeruginosa pao as the recipient. for one ecm plasmid, pmo61, the ability to form r-primes did not correlate with the ability to mobilize chromosomes in intrastrain crosses, suggesting that different mechanisms are involved. physical analysis of one r-pri ...	1987	2821167
in vivo formation of gene fusions in pseudomonas putida and construction of versatile broad-host-range vectors for direct subcloning of mu d1 and mu d2 fusions.	the mu d1 and mu d2 prophages were integrated into the conjugative broad-host-range plasmid r751. the two plasmids were then transferred into pseudomonas putida, and derivatives carrying intact mu prophages were recovered. after induction of mu at 42 degrees c, both operon and gene fusions were observed on 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (x-gal) plates. broad-host-range vectors were constructed which allow direct cloning of both operon or gene fusions and their analysis in es ...	1987	2821901
electron paramagnetic resonance study of ferrous cytochrome p-450scc-nitric oxide complexes: effects of cholesterol and its analogues.	electron paramagnetic resonance (epr) spectra of nitric oxide (no) complexes of ferrous cytochrome p-450scc were measured at 77 k for the first time without using the rapid-mixing and freeze-quenching technique. without substrate the epr spectra were very similar to those of cytochrome p-450cam (from pseudomonas putida) and cytochrome p-450lm (from rat liver microsomes) with rhombic symmetry; gx = 2.071, gz = 2.001, gy = 1.962, and az = 2.2 mt for 14no complexes. upon addition of substrates [suc ...	1987	2822097