Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of three distinct extradiol dioxygenases involved in mineralization of dibenzofuran by terrabacter sp. strain dpo360. | the dibenzofuran-degrading bacterial strain dpo360 represents a new species of the genus terrabacter together with the previously described dibenzofuran-mineralizing bacterial strain dpo1361 (k.-h. engesser, v. strubel, k. christoglou, p. fischer, and h. g. rast, fems microbiol. lett. 65:205-210, 1989; v. strubel, ph.d. thesis, university of stuttgart, stuttgart, germany, 1991; v. strubel, h. g. rast, w. fietz, h.-j. knackmuss, and k.-h. engesser, fems microbiol. lett. 58:233-238, 1989). two 2,3 ... | 1997 | 8981980 |
| binding of l-branched-chain amino acids causes a conformational change in bkdr. | bkdr is the positive transcriptional activator of the inducible bkd operon of pseudomonas putida. evidence is accumulating that l-branched-chain amino acids are the inducers of the operon, and the data obtained in this study show that they induce a conformational change in bkdr. addition of l-branched-chain amino acids increased the susceptibility of bkdr to trypsin with the cleavage between arg-51 and gln-52 on the c-terminal side of the dna-binding domain. l-valine also caused an increased flu ... | 1997 | 8982009 |
| organisation of the tmb catabolic operons of pseudomonas putida tmb and evolutionary relationship with the xyl operons of the tol plasmid pww0. | in pseudomonas putida (pp) tmb the genes involved in the catabolism of methyl-substituted aromatic hydrocarbons 1,2,4-trimethylbenzene, m- and p-xylene (tmb operon), are functionally and genetically homologous to the xyl genes of the plasmid pww0, but are chromosomally encoded. we have analysed by cloning. southern blotting and sequencing of selected regions the organisation of the tmb cluster. this analysis shows that the structural and regulatory genes of the tmb and xyl systems exhibit a high ... | 1996 | 8982087 |
| cotranscription of a gtpase gene from the cyanobacterium synechocystis pcc 6803 and a p-type ca(2+)-atpase gene. | a gtpase gene adjacent to the ca(2+)-atpase gene from synechocystis pcc 6803 has been sequenced. it encodes for a protein of 456 amino acids revealing high homology to so-called 50k proteins of bacillus subtilis and pseudomonas putida. cotranscription of gtpase and ca(2+)-atpase genes has been shown by reverse transcription pcr. | 1996 | 8982253 |
| biodegradation of hydrogen sulfide by a laboratory-scale immobilized pseudomonas putida ch11 biofilter. | a heterotrophic pseudomonas putida ch11 was isolated from livestock farming wastewater and applied for the treatment of h2s-containing gas. extensive tests including removal characteristics, metabolic products, and removal efficiencies of h2s by p. putida ch11 were examined in batch and continuous systems. the optimum ph required to remove hydrogen sulfide was found in the range of 6-8. the maximum removal rate and the saturation constant were calculated to be vm = 1.36 g s/day.kg dry bead and k ... | 1996 | 8983205 |
| luciferase-dependent, cytochrome p-450-catalyzed dehalogenation in genetically engineered pseudomonas. | to investigate the possibility of luciferase-dependent photoreduction of cytochrome p-450's in vivo, vibrio harveyi luciferase was coexpressed with the bacterial cytochrome p-450cam in pseudomonas putida. luciferase expression was under the control of the pm promoter from the meta-cleavage tol operon, incorporated into the chromosome by a mini tn5-mediated transposition. cytochrome p-450cam expression was controlled by the ptac-lac promoter on the broad host range vector pmmb206. both proteins w ... | 1996 | 8987475 |
| construction and use of recombinant escherichia coli strains for the synthesis of toluene cis-glycol. | the toluene dioxygenase genes derived from pseudomonas putida ncimb 11767 were subcloned from a previously constructed recombinant plasmid, pig, using puc18 as the cloning vector and e. coli tg2 as the host strain. the resulting strain, e. coli tg2 (p1/1), produced toluene cis-glycol when grown in lb broth or minimal medium in the presence of toluene. restriction mapping and partial dna sequencing provided evidence for the presence of orfs with extensive homology to parts of the tod operon from ... | 1996 | 8987488 |
| cometabolic degradation of 4-chlorophenol by alcaligenes eutrophus. | alcaligenes eutrophus was grown in batch cultures using either phenol as a sole substrate or mixtures of phenol and 4-chlorophenol. phenol was found to be the sole source for carbon and energy while 4-chlorophenol was utilized only as a cometabolite. maximum growth rates on phenol reached only 0.26 h-1, significantly below the growth rates reported earlier with pseudomonas putida. the cometabolite was found to decrease biomass yield and increase lag time before logarithmic growth occurred. both ... | 1996 | 8987646 |
| degradation of 3-nitrophenol by pseudomonas putida b2 occurs via 1,2,4-benzenetriol. | growth of pseudomonas putida b2 in chemostat cultures on a mixture of 3-nitrophenol and glucose induced 3-nitrophenol and 1,2,4-benzenetriol-dependent oxygen uptake activities. anaerobic incubations of cell suspensions with 3-nitrophenol resulted in complete conversions of the substrate to ammonia and 1,2,4-benzenetriol. this indicates that p. putida b2 degrades 3-nitrophenol via 1,2,4-benzenetriol, via a pathway involving a hydroxylaminolyase. involvement of this pathway in nitroaromatic metabo ... | 1996 | 8987889 |
| 3-hydroxyisobutyrate dehydrogenase from pseudomonas putida e23: purification and characterization. | the nad(+)-dependent 3-hydroxyisobutyrate dehydrogenase [ec 1.1.1.31] was purified to homogeneity from pseudomonas putida e23. the enzyme was a tetramer (molecular mass, 120 kda) consisted of identical subunits (molecular mass, 30 kda). the enzyme was specific for nad+ (km, 0.44 mm). the maximal activity was obtained at about ph 10. the enzyme was specific for the l-isomer of 3-hydroxyisobutyrate. in addition to l-3-hydroxyisobutyrate, l-serine, 2-methyl-dl-serine, and 3-hydroxypropionate were s ... | 1996 | 8988636 |
| nucleotide sequences and regulational analysis of genes involved in conversion of aniline to catechol in pseudomonas putida ucc22(ptdn1). | a 9,233-bp hindiii fragment of the aromatic amine catabolic plasmid ptdn1, isolated from a derivative of pseudomonas putida mt-2 (ucc22), confers the ability to degrade aniline on p. putida kt2442. the fragment encodes six open reading frames which are arranged in the same direction. their 5' upstream region is part of the direct-repeat sequence of ptdn1. nucleotide sequence of 1.8 kb of the repeat sequence revealed only a single base pair change compared to the known sequence of is1071 which is ... | 1997 | 8990291 |
| initial steps in the degradation of 3,4-dimethylbenzoic acid by pseudomonas putida strain dmb. | pseudomonas putida strain dmb capable of growing on 3,4-dimethylbenzoic acid as the only c and energy source was isolated by enrichment techniques. it does not utilize for growth or cooxidize the other dimethylbenzoate isomers tested. 3,4-dimethylsalicylic acid, 3,4-dimethylphenol and 3,4-dimethylcatechol were isolated and identified by nuclear magnetic resonance and mass spectra in the reaction mixture of p. putida washed cells. the detection of the two first metabolites suggests that the initi ... | 1996 | 8998974 |
| relative expression and stability of a chromosomally integrated and plasmid-borne marker gene fusion in environmentally competent bacteria. | a xyle-icec transcriptional fusion was created by ligatinga dna fragment harboring the cloned xyle structural gene from the tol plasmid of pseudomonas putida mt-2 into the cloned icec gene of pseudomonas syringae cit7. this fusion construct was integrated into the chromosome of pseudomonas syringae cit7 by homologous recombination. both cis-merodiploid strain cit7m17 and marker exchange strain cit7h69 produced the xyle gene product, catechol2,3-dioxygenase. strain cit7m17, in which xyle was infl ... | 1997 | 9003582 |
| neisseria meningitidis tonb, exbb, and exbd genes: ton-dependent utilization of protein-bound iron in neisseriae. | we have recently cloned and characterized the hemoglobin (hb) receptor gene, hmbr, from neisseria meningitidis. to identify additional proteins that are involved in hb utilization, the n. meningitidis hb utilization system was reconstituted in escherichia coli. five cosmids from n. meningitidis dna library enabled a heme-requiring (hema), hmbr-expressing mutant of e. coli to use hb as both porphyrin and iron source. nucleotide sequence analysis of dna fragments subcloned from the hb-complementin ... | 1997 | 9006036 |
| antigenic mapping of bacterial and animal cytochromes p-450. | a peptide scanning (pepscan) approach was used for antigenic mapping of two hepatic microsomal cytochromes p450 (rab1a2 and rab2b4) and the microbial cytochrome from pseudomonas putida (p450 101 or p450cam). this approach includes simultaneous synthesis of pin-linked overlapping hexapeptides covering the whole sequences of three p450s and testing them by elisa with corresponding polyclonal antisera. microsomal cytochrome p450 maps were shown to vary depending on an antiserum used for testing the ... | 1996 | 9010604 |
| [isolation of bacterial endotoxins from pseudomonas putida 36 and escherichia coli (vulgaris)]. | extracts of endotoxins from gram-negative bacteria pseudomonas putida 36 and escherichia coli (vulgaris) grown upon solid-phase or submerged cultivations were studied. the composition of the extracts was analyzed by gel-filtration. ps. putida 36 was shown to be a promising producer of bacterial endotoxins. | 1996 | 9011857 |
| molecular cloning of the nema gene encoding n-ethylmaleimide reductase from escherichia coli. | using the gene mapping membrane technique, we identified a gene (nema) that encodes n-ethylmaleimide reductase in escherichia coli. the open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 da. the deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of enterobacter cloacae and the morphinone reductase of pseudomonas putida (52% identical). | 1997 | 9013822 |
| mechanisms for solvent tolerance in bacteria. | the development of tolerance in pseudomonas putida dot-t1 to toluene and related highly toxic compounds involves short- and long-term responses. the short-term response is based on an increase in the rigidity of the cell membrane by rapid transformation of the fatty acid cis-9,10-methylene hexadecanoic acid (c17:cyclopropane) to unsaturated 9-cis-hexadecenoic acid (c16:1,9 cis) and subsequent transformation to the trans isomer. the long-term response involves in addition to the changes in fatty ... | 1997 | 9020089 |
| turn angle and run time distributions characterize swimming behavior for pseudomonas putida. | the swimming behavior of pseudomonas putida was analyzed with a tracking microscope to quantify its run time and turn angle distributions. monte carlo computer simulations illustrated that the bimodal turn angle distribution of p. putida reduced collisions with obstacles in porous media in comparison to the unimodal distribution of escherichia coli. | 1997 | 9023235 |
| construction and use of a versatile set of broad-host-range cloning and expression vectors based on the rk2 replicon. | the plasmid vectors described in this report are derived from the broad-host-range rk2 replicon and can be maintained in many gram-negative bacterial species. the complete nucleotide sequences of all of the cloning and expression vectors are known. important characteristics of the cloning vectors are as follows: a size range of 4.8 to 7.1 kb, unique cloning sites, different antibiotic resistance markers for selection of plasmid-containing cells, orit-mediated conjugative plasmid transfer, plasmi ... | 1997 | 9023917 |
| xyluw, two genes at the start of the upper pathway operon of tol plasmid pww0, appear to play no essential part in determining its catabolic phenotype. | the upper pathway operon of the toluene catabolic pathway of tol plasmid pww0 was shown to carry two open reading frames between the start of transcription and xylc (encoding benzaldehyde dehydrogenase), the first previously reported gene of the operon. these were designated xyluw: xylu encoded a protein of 131 amino acid residues (m(r) 14,244) which bore no relationship with any protein in the databases, and xylw encoded a protein of 348 residues (m(r) 36,992) which was strongly homologous to o ... | 1997 | 9025283 |
| a new approach for containment of microorganisms: dual control of streptavidin expression by antisense rna and the t7 transcription system. | the use of microorganisms in the open environment would be of less concern if they were endowed with programmed self-destruction mechanisms. here, we propose a new genetic design to increase the effectiveness of cell suicide systems. it ensures very tight control of the derepression of cell death by the combination of the bacteriophage t7 rna polymerase-lysozyme system and an inducible synthesis of antisense rna and the escherichia coli laci repressor. functionality of this regulatory concept wa ... | 1997 | 9037005 |
| a bacterial basic region leucine zipper histidine kinase regulating toluene degradation. | the two-component signal transduction pathways in bacteria use a histidine-aspartate phosphorelay circuit to mediate cellular changes in response to environmental stimuli. here we describe a novel two-component todst system, which activates expression of the toluene degradation (tod) pathway in pseudomonas putida f1. the tods gene is predicted to encode a sensory hybrid kinase with two unique properties--a basic region leucine zipper dimerization motif at the n terminus and a duplicated histidin ... | 1997 | 9037074 |
| anticancer efficacy of methioninase in vivo. | therapeutics that are selective for cancer would have a high potential for efficacy. we have previously shown that the metabolic defect of enhanced methionine dependence is a broad cancer-selective target. methionine depletion can completely arrest the growth of methionine-dependent tumor cells in vivo with a reversible pre-mitosis cell-cycle block. dietary methionine depletion can partially arrest the growth of methionine-dependent rodent tumors in vivo. this report demonstrates that methionina ... | 1996 | 9042315 |
| serum methionine depletion without side effects by methioninase in metastatic breast cancer patients. | the growth dependence of human tumors on elevated levels of methionine has been shown in preclinical in vitro and in vivo studies to be a frequently occurring, highly effective, tumor-selective therapeutic target. high purity endotoxin-free methioninase was produced from pseudomonas putida in order to develop anti-methionine chemotherapy targeting of human tumors. a pilot phase i clinical trial has been initiated in order to determine methioninase toxicity, the pharmacokinetics of methioninase a ... | 1996 | 9042316 |
| distribution of amine oxidases and amine dehydrogenases in bacteria grown on primary amines and characterization of the amine oxidase from klebsiella oxytoca. | the bacteria klebsiella oxytoca lmd 72.65 (atcc 8724), arthrobacter p1 lmd 81.60 (ncib 11625), paracoccus versutus lmd 80.62 (atcc 25364), escherichia coli w lmd 50.28 (atcc 9637), e. coli k12 lmd 93.68, pseudomonas aeruginosa pao1 lmd 89.1 (atcc 17933) and pseudomonas putida lmd 68.20 (atcc 12633) utilized primary amines as a carbon and energy source, although the range of amines accepted varied from organism to organism. the gram-negative bacteria k. oxytoca and e. coli as well as the gram-pos ... | 1997 | 9043125 |
| isolation and sequencing of a gene coding for glyoxalase i activity from salmonella typhimurium and comparison with other glyoxalase i sequences. | the glyoxalase i gene (gloa) from salmonella typhimurium has been isolated in escherichia coli on a multi-copy pbr322-derived plasmid, selecting for resistance to 3 mm methylglyoxal on luria-bertani agar. the region of the plasmid which confers the methylglyoxal resistance in e. coli was sequenced. the deduced protein sequence was compared to the known sequences of the homo sapiens and pseudomonas putida glyoxalase i (glxi) enzymes, and regions of strong homology were used to probe the national ... | 1997 | 9047352 |
| monitoring of biodegradative pseudomonas putida strains in aquatic environments using molecular techniques | 1997 | 9052646 | |
| overexpression and large-scale production of recombinant l-methionine-alpha-deamino-gamma-mercaptomethane-lyase for novel anticancer therapy. | the goal of the next generation of cancer chemotherapy is effective tumor-selectivity. a tumor-selective target with high therapeutic potential is the elevated methionine requirement of tumor cells relative to normal cells. we have termed the elevated requirement for methionine in tumors methionine dependence. to selectively target the methionine dependence of tumors for treatment on a large-scale preclinical and clinical basis, the l-methionine alpha-deamino-gamma-mercaptomethane-lyase (methion ... | 1997 | 9056489 |
| structural studies on the catalytic component of benzene dioxygenase from pseudomonas putida. | 1997 | 9056850 | |
| probing of pseudomonas aeruginosa, pseudomonas aureofaciens, burkholderia (pseudomonas) cepacia, pseudomonas fluorescens, and pseudomonas putida with the ferripyochelin receptor a gene and the synthesis of pyochelin in pseudomonas aureofaciens, pseudomonas fluorescens, and pseudomonas putida. | the ferripyochelin receptor a (fpta) gene codes for the transport of the ferrisiderophore ferripyochelin in pseudomonas aeruginosa. a p. aeruginosa fpta internal fragment was used to probe chromosomal dna from p. aureofaciens, b. cepacia, p. fluorescens, p. putida, and five strains of p.aeruginosa. these bacteria all contained dna that hybridized to the fpta fragment. four of the five p. aeruginosa strains displayed marked and identical patterns, indicating a high degree of sequence similarities ... | 1997 | 9058547 |
| the synthesis of (r)-(+)-lipoic acid using a monooxygenase-catalysed biotransformation as the key step. | 2-(2-acetoxyethyl)cyclohexanone (4) was converted into the lactone (-)-(5) regio- and enantioselectively using 2-oxo-delta 3-4,5,5-trimethylcyclopentenyl acetyl-coa monooxygenase, an nadph-dependent baeyer-villiger monooxygenase from camphor grown pseudomonas putida ncimb 10007. the lactone (-)-(5) was converted into (r)-(+)-lipoic acid in six steps. in contrast cyclopentanone monooxygenase, an nadph-dependent baeyer-villiger monooxygenase from cyclopentanol-grown pseudomonas sp. ncimb 9872 sele ... | 1997 | 9061190 |
| heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products. | the bpha1a2a3a4 gene cluster, encoding a biphenyl dioxygenase from rhodococcus globerulus p6, a gram-positive microorganism able to degrade a wide spectrum of polychlorobiphenyls (pcbs), was expressed in pseudomonas putida, thereby allowing characterization of chlorobiphenyl oxidation by this enzyme. while p6 biphenyl dioxygenase activity was observed in p. putida containing bpha1a2a3a4, no activity was detected in escherichia coli cells containing the same gene cluster. in e. coli, transcriptio ... | 1997 | 9068637 |
| transcriptional activation of the bkd operon of pseudomonas putida by bkdr. | reinvestigation of the transcriptional start site of the bkd operon of pseudomonas putida revealed that the transcriptional start site was located 86 nucleotides upstream of the translational start. there was a sigma 70 binding site 10 bp upstream of the transcriptional start site. the dissociation constants for bkdr, the transcriptional activator of the bkd operon, were 3.1 x 10(-7) m in the absence of l-valine and 8.9 x 10(-8) m in the presence of l-valine. binding of bkdr to substrate dna in ... | 1997 | 9068646 |
| chromosomal insertion of the entire escherichia coli lactose operon, into two strains of pseudomonas, using a modified mini-tn5 delivery system. | a 12-kb psti fragment including the entire e. coli lactose operon (lacipozya) was inserted in one copy into the chromosome of pseudomonas putida, pseudomonas fluorescens and an e. coli strain with lac- phenotype. this was made possible by improvements of an already existing mini-tn5 transposon delivery system (de lorenzo et al., 1990; herrero et al., 1990), which integrates cloned dna fragments at random sites on the chromosome of the recipient bacteria in single copies. this has resulted in: (a ... | 1997 | 9074492 |
| cloning and characterization of the exbb-exbd-tonb locus of pasteurella haemolytica a1. | a recombinant plasmid (pmg1) carrying pasteurella haemolytica a1 dna which complements a tonb mutation of escherichia coli has been isolated. e. coli tonb mete which carries pmg1 exhibits growth kinetics in the presence of vitamin b12 similar to that of the wild-type host. in addition, the complemented e. coli is susceptible to killing by bacteriophage phi 80 and colicin b. analysis of the nucleotide sequence in the complementing dna showed that it codes for three genes in the order of exbb-exbd ... | 1997 | 9074497 |
| expression and characterization of pseudomonas aeruginosa cytochrome c-551 and two site-directed mutants: role of tryptophan 56 in the modulation of redox properties. | the gene coding for pseudomonas aeruginosa cytochrome c-551 was expressed in pseudomonas putida under aerobic conditions, using two different expression vectors; the more efficient proved to be pnm185, induced by m-toluate. mature holo-(cytochrome c-551) was produced in high yield by this expression system, and was purified to homogeneity. comparison of the recombinant wild-type protein with that purified from ps. aeruginosa showed no differences in structural and functional properties. trp56, a ... | 1997 | 9078240 |
| pseudomonas putida b2: a tod-lux bioluminescent reporter for toluene and trichloroethylene co-metabolism. | a tod-lux transcriptional fusion bioluminescent reporter strain, pseudomonas putida b2, was developed to permit on-line analysis of trichloroethylene (tce) transformation by toluene dioxygenase (todc1c2ba) in pseudomonas putida f1. strain b2 was exposed to toluene in growing and resting cell bioluminescence assays. the growing cells showed a direct correlation between bioluminescence and toluene concentration, while resting cells showed reproducible bioluminescence with repeated toluene exposure ... | 1997 | 9079282 |
| activation of the catbca promoter: probing the interaction of catr and rna polymerase through in vitro transcription. | the soil bacterium pseudomonas putida is capable of degrading many aromatic compounds, including benzoate, through catechol as an intermediate. the catabolism of catechol is mediated by the catbca operon, whose induction requires the pathway intermediate cis,cis-muconate as an inducer and the regulatory protein, catr. catr also regulates the plasmid-borne pheba operon of p. putida paw85, which is involved in phenol catabolism. we have used an in vitro transcription system to study the roles of c ... | 1997 | 9079907 |
| tn5 tagging of the phenol-degrading gene on the chromosome of pseudomonas putida. | transposon mutagenesis was performed by the method of conjugational transfer in order to identify and characterize genes encoding enzymes involved in the pathway of phenol utilization as a carbon source. escherichia coli, which carries the tn5-132, was mated with pseudomonas putida sm25 as a host. we selected a mutant that could not utilize phenol as a carbon source. chromosomal integration of the transposon was confirmed by southern analysis, successfully tagging the gene related to a phenol-ut ... | 1997 | 9085263 |
| conservation of the multidrug resistance efflux gene oprm in pseudomonas aeruginosa. | an intragenic probe derived from the multidrug resistance gene oprm hybridized with genomic dna from all 20 serotypes of pseudomonas aeruginosa and from all 34 environmental and clinical isolates tested, indicating that the mexa-mexb-oprm multidrug efflux system is highly conserved in this organism. the oprm probe also hybridized with genomic dna from pseudomonas aureofaciens, pseudomonas chlororaphis, pseudomonas syringae, burkholderia pseudomallei, and pseudomonas putida, suggesting that efflu ... | 1997 | 9087505 |
| promoter-creating mutations in pseudomonas putida: a model system for the study of mutation in starving bacteria. | a novel experimental system to study mutation in starving bacteria was designed, relying on the activation of a promoterless phenol degradation operon of pseudomonas putida. the phe+ (phenol-utilizing) mutants accumulated in the starving culture of p. putida in the presence of phenol but not in the absence of it. we ruled out the possibility that the absence of phenol eliminates phe+ mutants from the starving population. sequence analysis of the phe+ mutants revealed that base substitutions, del ... | 1997 | 9096358 |
| choline monooxygenase, an unusual iron-sulfur enzyme catalyzing the first step of glycine betaine synthesis in plants: prosthetic group characterization and cdna cloning. | plants synthesize the osmoprotectant glycine betaine via the route choline --> betaine aldehyde --> glycine betaine. in spinach, the first step is catalyzed by choline monooxygenase (cmo), a ferredoxin-dependent stromal enzyme that has been hypothesized to be an oligomer of identical subunits and to be an fe-s protein. analysis by hplc and matrix-assisted laser desorption ionization ms confirmed that native cmo contains only one type of subunit (mr 42,864). determination of acid-labile sulfur an ... | 1997 | 9096415 |
| hrp pilus: an hrp-dependent bacterial surface appendage produced by pseudomonas syringae pv. tomato dc3000. | hypersensitive response and pathogenicity (hrp) genes control the ability of major groups of plant pathogenic bacteria to elicit the hypersensitive response (hr) in resistant plants and to cause disease in susceptible plants. a number of hrp proteins share significant similarities with components of the type iii secretion apparatus and flagellar assembly apparatus in animal pathogenic bacteria. here we report that pseudomonas syringae pv. tomato strain dc3000 (race 0) produces a filamentous surf ... | 1997 | 9096416 |
| genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of escherichia coli k-12. | we report the complete nucleotide sequence of the gene cluster encoding the 3-(3-hydroxyphenyl)propionate (3-hpp) catabolic pathway of escherichia coli k-12. sequence analysis revealed the existence of eight genes that map at min 8 of the chromosome, between the lac and hemb regions. six enzyme-encoding genes account for a flavin-type monooxygenase (mhpa), the extradiol dioxygenase (mhpb), and the meta-cleavage pathway (mhpcdfe). the order of these catabolic genes, with the sole exception of mhp ... | 1997 | 9098055 |
| coactivation in vitro of the sigma54-dependent promoter pu of the tol plasmid of pseudomonas putida by hu and the mammalian hmg-1 protein. | the mechanism by which the prokaryotic histone-like protein hu replaces the integration host factor (ihf) in the coactivation of the sigma54-dependent promoter pu of pseudomonas putida has been investigated. by using a preactivated form of the cognate activator protein xylr, we show that the functional replacement of ihf with hu previously suggested in vivo can be faithfully reproduced in vitro with purified components. furthermore, the coactivation effect of ihf on pu could be mimicked not only ... | 1997 | 9098077 |
| structures and characteristics of novel siderophores from plant deleterious pseudomonas fluorescens a225 and pseudomonas putida atcc 39167. | when pseudomonas putida atcc 39167 and plant-deleterious pseudomonas fluorescens a225 were grown in an iron-deficient culture medium, they each produced two different novel yellow-green fluorescent pseudobactins: p39167-i, ii and pa225-i, ii. pseudobactin p39167-i has a molecular formula of c46h65o23n13 and is monoanionic at neutral ph. p39167-ii has the molecular formula of c46h63o22n13 and no charge at neutral ph. pseudobactin pa225-i has a molecular formula of c46h65o24n13 and is monoanionic ... | 1997 | 9100010 |
| [production of indole-3-acetic acid by rhizosphere bacteria of the genus pseudomonas during the growth process]. | the production of indole-3-acetic acid (iaa) by batch cultures of the rhizosphere bacteria pseudomonas fluorescens 20 and pseudomonas putida 23, known to stimulate plant growth, was studied by immunoenzymatic analysis and the gordon-weber method. the accumulation of iaa exhibited two maxima, in the 9th and 40th hours of cultivation. in both pseudomonad cultures, the first maximum occurred in the exponential phase, and the second maximum corresponded to the beginning of the stationary growth phas ... | 1996 | 9102556 |
| microbulbifer hydrolyticus gen. nov., sp. nov., and marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. | two numerically important bacteria in marine pulp mill effluent enrichment cultures were isolated. these organisms were gram-negative, rod-shaped, strictly aerobic bacteria. isolate ire-31t (t = type strain) produced hydrolytic enzymes for the breakdown of cellulose, xylan, chitin, gelatin, and tween 80. it also utilized a variety of monosaccharides, disaccharides, amino acids, and volatile fatty acids for growth. isolate kw-40t did not utilize natural polymers, but it could grow on a variety of ... | 1997 | 9103623 |
| description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora. | a novel replicative origin (oriv) from a conjugative, mercury resistance plasmid (pqbr11, 304 kbp) has been cloned and sequenced. homology to the pqbr11 oriv-containing 3.55 kbp bamhi fragment (pcv1200) was restricted to one of five genetically distinct classes (group i) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. the oriv of pqbr11 was located within a unique sequence of 300 bp which initiated t ... | 1997 | 9103984 |
| genetic evidence of separate repressor and activator activities of the xylr regulator of the tol plasmid, pww0, of pseudomonas putida. | the xylr protein encoded by pww0, the tol (toluene biodegradation) plasmid of pseudomonas putida, activates at a distance the transcription of pu and ps, which are the two sigma(54)-dependent promoters of the plasmid, but it also downregulates its own sigma(70)-promoter, pr, which divergently overlaps the upstream activating sites of ps. all regulatory elements that control pr activity have been faithfully reproduced in escherichia coli, and the basis of the autoregulation of xylr transcription ... | 1997 | 9106213 |
| bipolar localization of a chromosome partition protein in bacillus subtilis. | we have determined the subcellular localization of the chromosome partition protein spo0j of bacillus subtilis by immunofluorescence microscopy and visualizing fluorescence of a spo0j-gfp fusion protein. spo0j was associated with a region of the nucleoid proximal to the cell pole, both in growing cells dividing symmetrically and in sporulating cells dividing asymmetrically. additional experiments indicated that spo0j was bound to sites in the origin-proximal third of the chromosome. these result ... | 1997 | 9114058 |
| comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soils. | five polycyclic aromatic hydrocarbon (pah) degrading bacterial strains, pseudomonas putida 34, pseudomonas fluorescens 62, pseudomonas aeruginosa 57, sphingomonas sp. strain 107, and the unidentified strain pl1, were isolated from two contaminated soils and characterized for specific features regarding pah degradation. degradation efficiency was determined by the rapidity to form clearing zones around colonies when sprayed with different pah solutions and the growth in liquid medium with differe ... | 1997 | 9115093 |
| cloning and nucleotide sequence of a d,l-haloalkanoic acid dehalogenase encoding gene from alcaligenes xylosoxidans ssp. denitrificans abiv. | we have cloned dna fragments of plasmid pfl40 from alcaligenes xylosoxidans ssp. denitrificans abiv encoding a d,l-2-haloalkanoic acid halidohydrolase (dhliv). a 6.5-kb ecori/sali-fragment with inducible expression of the halidohydrolase was cloned in pseudomonas fluorescens and escherichia coli. a 1.9-kb hindii-fragment demonstrated expression of the dehalogenase only due to the presence of the promoter from the puc vector in escherichia coli. the nucleotide sequence of this dna-fragment was de ... | 1996 | 9144969 |
| surface signaling: novel transcription initiation mechanism starting from the cell surface. | transcription of the ferric citrate transport genes of escherichia coli is induced by a novel mechanism. ferric citrate, the inducer, does not have to enter the cytoplasm to initiate transcription. interaction of ferric citrate with the outer membrane receptor protein feca induces transcription of the fec transport gene operon consisting of the fecirabcde genes. a signal from feca occupied with ferric citrate is transmitted across the outer membrane into the periplasm with the help of the electr ... | 1997 | 9148773 |
| p-cymene catabolic pathway in pseudomonas putida f1: cloning and characterization of dna encoding conversion of p-cymene to p-cumate. | pseudomonas putida f1 utilizes p-cymene (p-isopropyltoluene) by an 11-step pathway through p-cumate (p-isopropylbenzoate) to isobutyrate, pyruvate, and acetyl coenzyme a. the cym operon, encoding the conversion of p-cymene to p-cumate, is located just upstream of the cmt operon, which encodes the further catabolism of p-cumate and is located, in turn, upstream of the tod (toluene catabolism) operon in p. putida f1. the sequences of an 11,236-bp dna segment carrying the cym operon and a 915-bp dn ... | 1997 | 9150211 |
| a stereoselective cobalt-containing nitrile hydratase. | nitrile hydratase from pseudomonas putida nrrl-18668 has been purified and characterized. the purified enzyme catalyzes the hydration of 2(s)-(4'-chlorophenyl)-3-methylbutyronitrile at least fifty times faster than that of 2(r)-(4'-chlorophenyl)-3-methylbutyronitrile. this enzyme is a member of the class of nitrile hydratase that contains cobalt. visible absorption and cd spectra suggest the cobalt exists as a non-corrin low-spin co3+ ion in a tetragonally-distorted octahedral ligand field. chem ... | 1997 | 9154927 |
| construction of a catalytically active iron superoxide dismutase by rational protein design. | the rational protein design algorithm dezymer was used to introduce the active site of nonheme iron superoxide dismutase (sod) into the hydrophobic interior of the host protein, escherichia coli thioredoxin (trx), a protein that does not naturally contain a transition metal-binding site. reconstitution of the designed protein, trx-sod, showed the incorporation of one high-affinity metal-binding site. the electronic spectra of the holoprotein and its n3- and f- adducts are analogous to those prev ... | 1997 | 9159112 |
| catabolism of d-glucose by pseudomonas putida u occurs via extracellular transformation into d-gluconic acid and induction of a specific gluconate transport system. | pseudomonas putida u does not degrade d-glucose through the glycolytic pathway but requires (i) its oxidation to d-gluconic acid by a peripherally located constitutive glucose dehydrogenase (insensitive to osmotic shock), (ii) accumulation of d-gluconic acid in the extracellular medium, and (iii) the induction of a specific energy-dependent transport system responsible for the uptake of d-gluconic acid. this uptake system showed maximal rates of transport at 30 degrees c in 50 mm potassium phosp ... | 1997 | 9168611 |
| epitope mapping of cytochrome p450cam (cyp101). | eighteen linear antigenically active sites were revealed in cytochrome p450 from pseudomonas putida (p450cam) by hexapeptide scanning. these sites occupy about 31% of the protein sequence. hexapeptide epitope sequences of p450cam are not found in other cytochromes p450. however, several cytochromes p450 contain shorter fragments of p450cam epitope sequences which may cause weak immune cross-reactions. p450cam antigenic determinants are located generally at the boundaries of secondary structure e ... | 1997 | 9169009 |
| 2-oxo-1,2-dihydroquinoline 8-monooxygenase: phylogenetic relationship to other multicomponent nonheme iron oxygenases. | 2-oxo-1,2-dihydroquinoline 8-monooxygenase, an enzyme involved in quinoline degradation by pseudomonas putida 86, had been identified as a class ib two-component nonheme iron oxygenase based on its biochemical and biophysical properties (b. rosche, b. tshisuaka, s. fetzner, and f. lingens, j. biol. chem. 270:17836-17842, 1995). the genes oxor and oxoo, encoding the reductase and the oxygenase components of the enzyme, were sequenced and analyzed. oxor was localized approximately 15 kb downstream ... | 1997 | 9171399 |
| 2-chloromuconate and clcr-mediated activation of the clcabd operon: in vitro transcriptional and dnase i footprint analyses. | in pseudomonas putida, the plasmid-borne clcabd operon encodes enzymes involved in 3-chlorocatechol degradation. previous studies have demonstrated that these enzymes are induced when p. putida is grown in the presence of 3-chlorobenzoate, which is converted to 3-chlorocatechol, and that clcr, a lysr-type regulator, is required for this induction. the clcabd operon is believed to have evolved from the chromosomal catbca operon, which encodes enzymes that utilize catechol and is regulated by catr ... | 1997 | 9171413 |
| evidence for the bacterial origin of genes encoding fermentation enzymes of the amitochondriate protozoan parasite entamoeba histolytica. | entamoeba histolytica is an amitochondriate protozoan parasite with numerous bacterium-like fermentation enzymes including the pyruvate:ferredoxin oxidoreductase (por), ferredoxin (fd), and alcohol dehydrogenase e (adhe). the goal of this study was to determine whether the genes encoding these cytosolic e. histolytica fermentation enzymes might derive from a bacterium by horizontal transfer, as has previously been suggested for e. histolytica genes encoding heat shock protein 60, nicotinamide nu ... | 1997 | 9171424 |
| natural horizontal transfer of a naphthalene dioxygenase gene between bacteria native to a coal tar-contaminated field site. | horizontal transfer of genes responsible for pollutant biodegradation may play a key role in the evolution of bacterial populations and the adaptation of microbial communities to environmental contaminants. however, field evidence for horizontal gene transfer between microorganisms has traditionally been very difficult to obtain. in this study, the sequences of the 16s rrna and naphthalene dioxygenase iron-sulfur protein (nahac) genes of nine naphthalene-degrading bacteria isolated from a coal t ... | 1997 | 9172352 |
| conjugative plasmids and the degradation of arylsulfonates in comamonas testosteroni. | comamonas testosteroni t-2 degrades p-toluenesulfonate (tsa) via p-sulfobenzoate (psb) and protocatechuate and degrades toluenecarboxylate via terephthalate (ter) and protocatechuate. the appropriate genes are expressed in at least five regulatory units, some of which are also found in c. testosteroni psb-4 (f. junker, r. kiewitz, and a. m. cook, j. bacteriol. 179:919-927, 1997). c. testosteroni t-2 was found to contain two plasmids, ptsa (85 kbp) and pt2t (50 kbp); a tsa- mutant (strain ter-1) ... | 1997 | 9172362 |
| oxidation of aliphatic olefins by toluene dioxygenase: enzyme rates and product identification. | toluene dioxygenase from pseudomonas putida f1 has been studied extensively with aromatic substrates. the present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene as substrates for toluene dioxygenase. enzyme specific activities were determined for the more water-soluble c2 to c5 compounds and ranged from <4 to 52 nmol per min per mg of protei ... | 1997 | 9190800 |
| molecular characterization of the mde operon involved in l-methionine catabolism of pseudomonas putida. | a 15-kb region of pseudomonas putida chromosomal dna containing the mde operon and an upstream regulatory gene (mder) has been cloned and sequenced. the mde operon contains two structural genes involved in l-methionine degradative metabolism: the already-identified mdea, which encodes l-methionine gamma-lyase (h. inoue, k. inagaki, m. sugimoto, n. esaki, k. soda, and h. tanaka. j. biochem. (tokyo) 117:1120-1125, 1995), and mdeb, which encodes a homologous protein to the homodimeric-type e1 compo ... | 1997 | 9190812 |
| stereochemical course of two arene-cis-diol dehydrogenases specifically induced in pseudomonas putida. | catabolism of nonphenolic arenes is frequently initiated by dioxygenases, yielding single isomer products with two adjacent hydroxylated asymmetric centers. the next enzymic reaction dehydrogenates these cyclic cis-diols, with aromatization yielding catechols for ring cleavage. there are two stereochemical questions to answer. (i) to which face of nad is hydride transferred giving nadh? (ii) which hydrogen of the arene-cis-diols is donated to nad? we report the results of 1h nuclear magnetic res ... | 1997 | 9190820 |
| studies on spontaneous promoter-up mutations in the transcriptional activator-encoding gene phir and their effects on the degradation of phenol in escherichia coli and pseudomonas putida. | the activator-encoding gene phlr was identified upstream of the plasmid-encoded operon for phenol degradation in pseudomonas putida strain h by cassette mutagenesis and dna sequence analysis. the deduced amino acid sequence of phlr shows high homology to dmpr of p. putida sp. cf600 and to the chromosomally encoded phhr of p. putida p35x reported previously. trans-activation of phenol degradation was observed when phlr was overexpressed in a phlr insertion mutant. transconjugants of escherichia c ... | 1997 | 9197413 |
| modulation of gene expression through chromosomal positioning in escherichia coli. | variations in expression of the nah genes of the nah7 (naphthalene biodegradation) plasmid of pseudomonas putida when placed in different chromosomal locations in escherichia coli have been studied by employing a collection of hybrid mini-t5 transposons bearing lacz fusions to the psal promoter, along with the cognate regulatory gene nahr. insertions of psal-lacz reporters in the proximity of the chromosomal origin of replication, oric, increased accumulation of beta-galactosidase in vivo. posit ... | 1997 | 9202482 |
| construction of a contiguous 874-kb sequence of the escherichia coli -k12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. | the contiguous 874.423 base pair sequence corresponding to the 50.0-68.8 min region on the genetic map of the escherichia coli k-12 (w3110) was constructed by the determination of dna sequences in the 50.0-57.9 min region (360 kb) and two large (100 kb in all) and five short gaps in the 57.9-68.8 min region whose sequences had been registered in the dna databases. we analyzed its sequence features and found that this region contained at least 894 potential open reading frames (orfs), of which 34 ... | 1997 | 9205837 |
| phospholipid biosynthesis and solvent tolerance in pseudomonas putida strains. | the role of the cell envelope in the solvent tolerance mechanisms of pseudomonas putida was investigated. the responses of a solvent-tolerant strain, p. putida idaho, and a solvent-sensitive strain, p. putida mw1200, were examined in terms of phospholipid content and composition and of phospholipid biosynthetic rate following exposure to a nonmetabolizable solvent, o-xylene. following o-xylene exposure, p. putida mw1200 exhibited a decrease in total phospholipid content. in contrast, p. putida i ... | 1997 | 9209036 |
| bacterial dl-2-haloacid dehalogenase from pseudomonas sp. strain 113: gene cloning and structural comparison with d- and l-2-haloacid dehalogenases. | dl-2-haloacid dehalogenase from pseudomonas sp. strain 113 (dl-dex) catalyzes the hydrolytic dehalogenation of both d- and l-2-haloalkanoic acids to produce the corresponding l- and d-2-hydroxyalkanoic acids, respectively, with inversion of the c2 configuration. dl-dex is a unique enzyme: it acts on the chiral carbon of the substrate and uses both enantiomers as equivalent substrates. we have isolated and sequenced the gene encoding dl-dex. the open reading frame consists of 921 bp corresponding ... | 1997 | 9209038 |
| tolerance to mercury chloride in scenedesmus strains. | mercury chloride toxicity was investigated in two strains of chlorella and in a strain of scenedesmus isolated from polluted areas in tuscany (italy). no hg resistance was found in the autotrophic microorganisms isolated, but scenedesmus sp. strain ar-2489, isolated from the arno river, was able to grow at concentrations of up to 5 micrograms ml-1 of hg. this concentration was twice that which inhibited growth of the two chlorella strains and scenedesmus acutus 8m, the reference strain from a cu ... | 1997 | 9210291 |
| fiber-optic-based biomonitoring of benzene derivatives by recombinant e. coli bearing luciferase gene-fused tol-plasmid immobilized on the fiber-optic end. | tol plasmid in pseudomonas putida mt-2 has a series of genes for the degradation of xylene, toluene, and their derivatives to pyruvate and acetaldehyde (or propionaldehyde). two operons, i.e., upper operon and meta operon, play indispensable roles for the digestion of xylene derivatives: when xyir protein recognizes xylene derivatives, another controlling gene, xyis, is activated, which results in the activation of meta operon. therefore, we have constructed a fusion gene between tol plasmid and ... | 1997 | 9212714 |
| microbial contamination of antiseptic-soaked cotton balls. | we investigated microbial contamination of in-use antiseptics at a hospital. no microbial contamination was observed in 70 samples of 0.02% benzalkonium chloride solution (500-ml volume), 70 samples of 1% titratable i2 povidone-iodine solution (250-ml volume), or 15 samples of 0.1% ethacridine lactate solution (500-ml volume) during use in reduced amounts. nor was any microbial contamination observed in 70 samples of cotton balls soaked in 1% titratable i2 povidone-iodine solution in canisters o ... | 1997 | 9212987 |
| detection and molecular analysis of plant- and insect-associated bacteria harboring aconitate isomerase involved in biosynthesis of trans-aconitic acid as antifeedant in brown planthoppers. | the activity of aconitate isomerase, which is involved in the biosynthesis of trans-aconitic acid as antifeedant in brown planthoppers, was detected in pseudomonas fluorescens lrb3w1 and pseudomonas putida maff301685 but not in pseudomonas putida maff301684. the enzyme activity was induced in the presence of trans-aconitate, and therefore bacteria showing the enzyme activity were easily detected by their ability to grow on the minimal medium containing trans-aconitate as the sole carbon source ( ... | 1997 | 9216883 |
| dnase i footprinting, dna bending and in vitro transcription analyses of clcr and catr interactions with the clcabd promoter: evidence of a conserved transcriptional activation mechanism. | in pseudomonas putida, benzoate and 3-chlorobenzoate are converted to catechol and 3-chlorocatechol, respectively, which are then catabolized to tricarboxylic acid cycle intermediates via the catbca and clcabd pathways. the catbca and clcabd operons are regulated by homologous transcriptional activators catr and clcr. previous studies have demonstrated that in addition to sequence similarities, catr and clcr share functional similarities which allow catr to complement clcr. in this study, we dem ... | 1997 | 9220004 |
| cloning and analysis of the dnag gene encoding pseudomonas putida dna primase. | the dnag gene coding for primase, a key enzyme in dna replication, has been isolated from chromosomal dna of the soil bacterium pseudomonas putida. it maps within the putative mms operon, between the rpsu and rpod genes. comparison of the deduced amino acid sequence of p. putida dnag with sequences of other known bacterial primases reveals the presence of a possible regulatory region which would be unique to pseudomonads. the analysis of nucleotide sequence suggests that stable folding of the dn ... | 1997 | 9224947 |
| microbial degradation of chloroaromatics: use of the meta-cleavage pathway for mineralization of chlorobenzene. | pseudomonas putida gj31 is able to simultaneously grow on toluene and chlorobenzene. when cultures of this strain were inhibited with 3-fluorocatechol while growing on toluene or chlorobenzene, 3-methylcatechol or 3-chlorocatechol, respectively, accumulated in the medium. to establish the catabolic routes for these catechols, activities of enzymes of the (modified) ortho- and meta-cleavage pathways were measured in crude extracts of cells of p. putida gj31 grown on various aromatic substrates, i ... | 1997 | 9226262 |
| pseudomonas monteilii sp. nov., isolated from clinical specimens. | we propose the name pseudomonas monteilii for a new species of gram-negative, rod-shaped, motile bacteria that were nonhemolytic on blood agar and were isolated from clinical sources. the 10 strains of p. monteilii were incapable of liquefing gelatin. they grew at 10 degrees c but not at 41 degrees c, produced fluorescent pigments, catalase, and cytochrome oxidase, and possessed the arginine dihydrolase system. they were capable of respiratory but not fermentative metabolism. they did not hydrol ... | 1997 | 9226917 |
| rapid purification of an active recombinant his-tagged 2,3-dihydroxybiphenyl 1,2-dioxygenase from pseudomonas putida ou83. | 2,3-dihydroxybiphenyl 1,2-dioxygenase (2,3-dbpd) is an extradiol-type dioxygenase that catalyzes the aromatic ring fission of 2,3-dihydroxybiphenyl, the third step in the biphenyl degradation pathway. the nucleotide sequence of the pseudomonas putida ou83 gene bphc, which encodes 2,3-dbpd, was cloned into a plasmid pqe31. the his-tagged 2,3-dbpd produced by a recombinant escherichia coli strain, sg13009(prep4)(pakc1), and purified with a ni-nitrilotriacetic acid resin affinity column using the h ... | 1997 | 9228766 |
| synthesis of fragments of the peptide component of pseudobactin. | pseudobactin is a structurally complex and physiologically important siderophore (microbial iron chelator] from pseudomonas putida-fluorescens. various fragments of the unusual peptide component of pseudobactin listed below were prepared by solution-phase peptide synthesis. l-lys.d-threo-beta-oh asp.l-ala.d-allo-thr.l-ala l-lys.d-threo-beta oh asp.l-ala.d-allo-thr d-threo-beta-oh asp.l-ala.d-allo-thr.l-ala.d-n-oh-cycloorn d-threo-beta-oh-asp.l-ala.d-allo-thr.l-ala l-ala.d-allo-thr.l-ala.d-n-oh-c ... | 1996 | 9231324 |
| contamination of diagnostic ophthalmic solutions in primary eye care settings. | pharmaceutical agents and irrigating solutions are widely used in both optometric and ophthalmologic practices. contamination of these containers or solutions could possibly pose some risk of infection to a patient. we set out to investigate the possible contamination of a representative sample of these containers in small office practices. representative bottles of two diagnostic pharmaceutical agents and an irrigating solution were obtained from primary care optometric and ophthalmologic pract ... | 1997 | 9232983 |
| preferential interaction of the his pause rna hairpin with rna polymerase beta subunit residues 904-950 correlates with strong transcriptional pausing. | rna secondary structures (hairpins) that form as the nascent rna emerges from rna polymerase are important components of many signals that regulate transcription, including some pause sites, all rho-independent terminators, and some antiterminators. at the his leader pause site, a 5-bp-stem, 8-nt-loop pause rna hairpin forms 11 nt from the rna 3' end and stabilizes a transcription complex conformation slow to react with ntp substrate. this stabilization appears to depend at least in part on an i ... | 1997 | 9237994 |
| cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of aeromonas caviae. | a 5.0-kbp ecorv-ecori restriction fragment was cloned and analyzed from genomic dna of aeromonas caviae, a bacterium producing a copolyester of (r)-3-hydroxybutyrate (3hb) and (r)-3-hydroxyhexanoate (3hhx) [p(3hb-co-3hhx)] from alkanoic acids or oils. the nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (pha) synthase gene (phac(ac) [i.e., the phac gene from a. caviae]) together with four open reading frames (orf1, -3, -4, and -5) and one putative promoter region. t ... | 1997 | 9244271 |
| importance of biofilm formation for corrosion inhibition of sae 1018 steel by axenic aerobic biofilms. | to investigate if corrosion inhibition by aerobic biofilms is a general phenomenon, carbon steel (sae 1018) coupons were exposed to a complex liquid medium (luria-bertani) and seawater-mimicking medium (vnss) containing fifteen different pure-culture bacterial suspensions representing seven genera. compared to sterile controls, the mass loss in the presence of these bacteria (which are capable of developing a biofilm to various degrees) decreased by 2- to 15-fold. the extent of corrosion inhibit ... | 1997 | 9248069 |
| physiological stress in batch cultures of pseudomonas putida 54g during toluene degradation. | physiological stress associated with toluene exposure in batch cultures of pseudomonas putida 54g was investigated. p. putida 54g cells were grown using a continuous vapor phase feed stream containing 150 ppmv or 750 ppmv toluene as the sole carbon and energy source. cells were enumerated on non-selective (r2a agar plates) and a selective minimal medium incubated in the presence of vapor phase toluene (hcmm2). differential recovery on the two media was used to evaluate bacterial stress, culturab ... | 1997 | 9248070 |
| characteristics of escherichia coli hb101 and pseudomonas putida ppy101 harboring a recombinant plasmid with tandem insertion of the mercury resistance operon. | we constructed the plasmid psupmer2 by inserting tandem copies of the mercury resistance (mer) operon into a broad host range-vector, and introduced it into escherichia coli hb101 and pseudomonas putida ppy101 to increase their mercury resistance. strains harboring plasmid psupmer2 had higher mercury resistance and mercuric reductase activity than those strains harboring the plasmid psupmer which had one copy of the mer operon. mercury resistance of p. putida ppy101 was significantly increased b ... | 1997 | 9255983 |
| polymerase c1 levels and poly(r-3-hydroxyalkanoate) synthesis in wild-type and recombinant pseudomonas strains. | a functional antibody highly specific for polymerase c1 of pseudomonas oleovorans gpo1 was raised and used to determine polymerase c1 levels in in vivo experiments. the polymerase c1 antibodies did not show a cross-reaction with polymerase c2 of p. oleovorans. in wild-type p. oleovorans gpo1 and pseudomonas putida kt2442, amounts of 0.075 and 0.06% polymerase relative to total protein, respectively, were found. p. oleovorans gpo1(pgec405), which contained additional copies of the polymerase c1-e ... | 1997 | 9260937 |
| transcriptional control of the multiple catabolic pathways encoded on the tol plasmid pww53 of pseudomonas putida mt53. | the tol plasmid pww53 encodes a catabolic pathway for the metabolism of toluene. it bears an upper-pathway operon for the oxidation of toluene to benzoate and a copy of the gene that encodes regulatory protein xylr. for metabolism of the aromatic carboxylic acid, it bears two functional homologous meta-pathway operons, together with two functional copies of the xyls regulatory gene (xyls1 and xyls3). in cells growing in the absence of pathway substrates, no mrna from upper- and meta-pathway oper ... | 1997 | 9260942 |
| pcak, a high-affinity permease for the aromatic compounds 4-hydroxybenzoate and protocatechuate from pseudomonas putida. | pcak is a transporter and chemoreceptor protein from pseudomonas putida that is encoded as part of the beta-ketoadipate pathway regulon for aromatic acid degradation. when expressed in escherichia coli, pcak was localized to the membrane and catalyzed the accumulation of two aromatic substrates, 4-hydroxybenzoate and protocatechuate, against a concentration gradient. benzoate inhibited 4-hydroxybenzoate uptake but was not a substrate for pcak-catalyzed transport. a p. putida pcak mutant was defe ... | 1997 | 9260946 |
| cloning and mutational analysis of the gene for the stationary-phase inducible catalase (catc) from pseudomonas putida. | pseudomonas putida, a bacterium that colonizes plant roots and enhances plant growth, produces three isozymes of catalase (a, b, and c) in stationary-phase cells. a catalase probe, generated by pcr analysis of p. putida genomic dna with oligomers based on typical catalase sequences, hybridized to a genomic clone that expressed catalase c in escherichia coli. the catc gene from this clone had a 2,133-bp open reading frame with a high level of identity to the stationary-phase-specific e. coli kate ... | 1997 | 9260972 |
| group ii intron from pseudomonas alcaligenes ncib 9867 (p25x): entrapment in plasmid rp4 and sequence analysis. | pseudomonas alcaligenes ncib 9867 (strain p25x), which grows on 2,5-xylenol and harbours the plasmid rp4, was mated with a plasmid-free derivative of pseudomonas putida ncib 9869, strain ra713, which cannot grow on 2,5-xylenol. some ra713 transconjugants, initially selected on 2,5-xylenol, were found to carry rp4 plasmids that had acquired additional fragments (designated xin) which ranged in size from 2 kb to approximately 26 kb instability of dna inserts in rp4::xin hybrid plasmids was observe ... | 1997 | 9274037 |
| characterization and optimization of a two-phase partitioning bioreactor for the biodegradation of phenol. | a two-phase partitioning bioreactor containing pseudomonas putida atcc 11172 was used to degrade high concentrations of phenol in batch and fed-batch mode. the 2-1 (nominal volume) partitioning bioreactor employs a 1-1 cell-containing aqueous phase, and a 500-ml immiscible and biocompatible second organic phase (2-undecanone), which partitions the toxic substrate into the aqueous phase at a rate based on the metabolic activity of the microorganisms. using this reactor configuration, operated in ... | 1997 | 9274043 |
| crystallization and preliminary x-ray diffraction studies of expressed pseudomonas putida catechol 2,3-dioxygenase. | crystals of recombinant pseudomonas putida catechol 2,3-dioxygenase, metapyrocate-chase, composed of four identical subunits, each with a molecular mass of 35 kda and one nonheme ferrous iron, have been grown by the vapor diffusion method using sodium citrate as the precipitant. repeated macroseeding and the addition of ethanol to protein solutions were together effective for obtaining crystals suitable for further crystallographic characterization. the crystals belong to the tetragonal space gr ... | 1997 | 9276689 |
| improved broad-host-range rk2 vectors useful for high and low regulated gene expression levels in gram-negative bacteria. | this report describes the construction and use of improved broad-host-range expression vectors based on the previously constructed pjb137 and pjb653 plasmids (blatny et al., 1997). these vectors contain the minimal replicon of rk2 and the inducible pu or pm promoters together with their regulatory xylr or xyls genes, respectively, from the pseudomonas putida tol plasmid pwwo. a set of atg vectors were derived from pjb653, and these vectors are characterized by the relatively small size, the pres ... | 1997 | 9281494 |
| bacterial resistance to vancomycin: overproduction, purification, and characterization of vanc2 from enterococcus casseliflavus as a d-ala-d-ser ligase. | the vanc phenotype for clinical resistance of enterococci to vancomycin is exhibited by enterococcus gallinarum and enterococcus casseliflavus. based on the detection of the cell precursor udp-n-acetylmuramic acid pentapeptide intermediate terminating in d-ala-d-ser instead of d-ala-d-ala, it has been predicted that the vanc ligase would be a d-ala-d-ser rather than a d-ala-d-ala ligase. overproduction of the e. casseliflavus atcc 25788 vanc2 gene in escherichia coli and its purification to homo ... | 1997 | 9294159 |
| crystal structure and resonance raman studies of protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate. | the crystal structure of the anaerobic complex of pseudomonas putida protocatechuate 3,4-dioxygenase (3,4-pcd) bound with the alternative substrate, 3,4-dihydroxyphenylacetate (hpca), is reported at 2.4 a resolution and refined to an r factor of 0.17. formation of the active site fe(iii).hpca chelated complex causes the endogenous axial tyrosinate, tyr447 (147beta), to dissociate from the iron and rotate into an alternative orientation analogous to that previously observed in the anaerobic 3,4-p ... | 1997 | 9298971 |
| cloning of the phosphonoacetate hydrolase gene from pseudomonas fluorescens 23f encoding a new type of carbon-phosphorus bond cleaving enzyme and its expression in escherichia coli and pseudomonas putida. | the phna gene encoding a novel carbon-phosphorus bond cleavage enzyme, phosphonoacetate hydrolase, from pseudomonas fluorescens 23f was cloned and expressed in escherichia coli and pseudomonas putida. it conferred on the latter host the ability to mineralize phosphonoacetate but on the former the ability to utilize it as sole phosphorus source only. the nucleotide and deduced amino acid sequences of the phna gene showed no significant homology with any data bank accessions. | 1997 | 9300819 |