Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| p-cymene pathway in pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs. | several classes of mutants of pseudomonas putida (jt810) defective in the utilization of p-cymene as sole carbon source have been isolated. selective enrichment of the mutants and for strains putatively cured of a degradative plasmid was achieved by incubation of cells in minimal growth media containing p-cymene (or p-cumate) and various halogenated analogs of the growth substrates or pathway intermediates. analogs which led to successful enrichments included: p-chlorotoluene, p-bromotoluene, al ... | 1980 | 7204334 |
| purification and properties of nadh-ferredoxintol reductase. a component of toluene dioxygenase from pseudomonas putida. | cells of pseudomonas putida, after growth with toluene, contain a multicomponent enzyme system that oxidizes toluene to (+)-1(s),2(r)-dihydroxy-3-methyl-cyclohexa-3,5-diene. one of these components has been purified to homogeneity and shown to be a flavoprotein that contains fad as the only detectable prosthetic group. fad was removed from the enzyme during purification. however, equilibrium dialysis experiments showed that the enzyme can bind one mol of fad/mol of enzyme protein. the apparent m ... | 1981 | 7204373 |
| mechanism of salicylate hydroxylase-catalyzed decarboxylation. | salicylate hydroxylase (salicylate, nadh: oxygen oxidoreductase (1-hydroxylating, decarboxylating), ec 1.14.13.1) in pseudomonas putida catalyzed hydroxylation of the substrate analogue, salicylaldehyde, to form catechol and formate with stoichiometric consumption of nadh and o2. consequently, a study of primary product derived from the carboxyl group of the authentic substrate, salicylate, was undertaken. the experimental results revealed that co2 not h2co3, was produced first. | 1981 | 7213760 |
| catabolism of pseudocumene and 3-ethyltoluene by pseudomonas putida (arvilla) mt-2: evidence for new functions of the tol (pwwo) plasmid. | pseudocumene (1,2,4-trimethylbenzene) and 3-ethyltoluene were found to serve as growth substrates for pseudomonas putida (arvilla) mt-2, in addition to toluene, m-xylene, and p-xylene as previously described. similar observations were made with several additional p. putida strains also capable of growth with toluene and the xylenes. additional substrates which supported the growth of these organisms included 3,4-dimethylbenzyl alcohol, 3,4-dimethylbenzoate, and 3-ethylbenzoate. p. putida mt-2 ce ... | 1981 | 7216999 |
| plasmid specifying total degradation of 3-chlorobenzoate by a modified ortho pathway. | a plasmid, termed pac25, specifying biodegradation of 3-chlorobenzoate in a strain of pseudomonas putida has been characterized. during growth of the plasmid-harboring cells with 3-chlorobenzoate, there was an accumulation of 3-chlorocatechol and beta-chloromuconic acid as intermediates and release of more than 80% of the chlorine in the form of inorganic chloride. the plasmid had a mean molecular mass of 68 x 10(6) daltons and was transmissible to a number of pseudomonas species such as p. aeru ... | 1981 | 7217013 |
| biological distribution and physiological role of the beta-ketoadipate transport system. | beta-ketoadipate induces catabolic enzymes in pseudomonas putida. the compound is transported by a system which also concentrates adipate, a non-metabolizable analogue of beta-ketoadipate. the natural substrate, beta-ketoadipate, competitively inhibits adipate transport with a k1 of 0.04 mm, lower than the km of 0.23 mm observed with adipate. transport is inhibited competitively by succinate (k1 1.3 mm) and non-competitively by acetate (k1 5.3 mm). the system has a sharp ph optimum at 5.5. trans ... | 1980 | 7217919 |
| [arsenic oxidation by the heterotrophic bacteria pseudomonas putida and alcaligenes eutrophus]. | two heterotrophic bacteria, pseudomonas putida 18 and alcaligenes eutrophus 280, were isolated from gold-arsenic deposits. the bacteria oxidize as(iii) to as(v) at ph 6-9 and temperatures 4-28 and 28 degrees c respectively. the oxidation is accompanied by a decrease in the ph of the medium. the rate of the oxidation directly depends on the number of cells in the inoculum. | 1981 | 7219219 |
| [the identification of nonfermentative gram-negative bacteria. experiences with 676 apyocyaninogenic strains (author's transl)]. | during a period of 16 months 1757 strains of nonfermentative gram-negative rods have been isolated from clinical material. of the, 1205 (69%) were p. aeruginosa, 124 (10%) of which failed to produce pyocyanin. the apyocyaninogenic strains as well as the remaining 552 isolates were differentiated by steps according to a diagnostic scheme developed by us. for identification of species two or three steps were needed. by this procedure, 530 of the 552 strains could be assigned to nineteen species wi ... | 1981 | 7223132 |
| isolation and characterization of spontaneously occurring tol plasmid mutants of pseudomonas putida hs1. | a strain of pseudomonas (p. putida hs1) was found to resemble p. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. the ability to degrade these substrates by p. putida hs1 (ppc1) was shown to be encoded by a tol (pdk1) plasmid as evidenced by: (i) spontaneous loss of the tol-related phenotype after growth with benzoate, (ii) transfer o ... | 1981 | 7240090 |
| interaction of 5-bromocamphor with cytochrome p-450 cam. production of 5-ketocamphor from a mixed spin state hemoprotein. | camphor is stereospecifically hydroxylated by the soil bacterium pseudomonas putida at the 5-exo position by a cytochrome p-450 mixed function oxidase system consisting of a flavoprotein reductase; putidaredoxin, an iron-sulfur oxidation-reduction transport-effector protein; and the p-450 hemoprotein. we have studied the interaction of a substrate analog of camphor, 5-exo-bromocamphor, with this cytochrome p-450 mixed function oxidase system in order to probe the molecular mechanisms of electron ... | 1981 | 7240237 |
| reconstitution of iron-sulfur cluster of nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1. | nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system, is an ion-sulfur flavoprotein with one fad and one iron-sulfur cluster of [2fe-2s] type (yamaguchi, m., and fujisawa, h. (1978) j. biol. chem. 253, 8848-8853). treatment of nadh-cytochrome c reductase with p-chloromercuriphenylsulfonic acid resulted in fading of its color with a concomitant loss of the nadh-cytochrome c reductase activity. the p-chloromercuriphenylsulfonic acid-treated enzyme was found to contain one fa ... | 1981 | 7240244 |
| 8 alpha-(o-tyrosyl)flavin adenine dinucleotide, the prosthetic group of bacterial p-cresol methylhydroxylase. | 8 alpha-(o-tyrosyl)riboflavin has been synthesized by condensation of the copper complex of l-tyrosine with 8 alpha-bromotetraacetylriboflavin. the structure of this synthetic product was proven by absorption and 1h nmr spectroscopy and by chemical degradation, which yielded 1 mol of tyrosine per mol of flavin. the synthetic compound comigrated wtih the (aminoacyl)riboflavin isolated from the p-cresol methylhydroxylase of pseudomonas putida, and both showed identical absorption and fluorescence ... | 1981 | 7248267 |
| d-glucose and d-gluconate transport in vesicles from pseudomonas putida. | vesicles prepared from glucose-grown cells of pseudomonas putida (atcc, 12633) retain glucose oxidase (gox) and gluconate dehydrogenase (gadh) activity and actively transport d-glucose, 2-deoxy-d-glucose (2dog), 3-deoxy-3-fluoro-d-glucose (3fg), and d-gluconate by saturable processes. the transport of these substrates is stimulated by the addition of l-malate or reduced phenazine methosulphate (pms). vesicles prepared from succinate-grown cells of p. putida lose their capacity to transport d-glu ... | 1980 | 7248836 |
| inhibition of catechol 2,3-dioxygenase from pseudomonas putida by 3-chlorocatechol. | partially purified preparations of catechol 2,3-dioxygenase from toluene-grown cells of pseudomonas putida catalyzed the stoichiometric oxidation of 3-methylcatechol to 2-hydroxy-6-oxohepta-2,4-dienoate. other substrates oxidized by the enzyme preparation were catechol, 4-methylcatechol, and 4-fluorocatechol. the apparent michaelis constants for 3-methylcatechol and catechol were 10.6 and 22.0 mum, respectively. substitution at the 4-position decreases the affinity and activity of the enzyme for ... | 1981 | 7259155 |
| bacterial degradation of 3,4,5-trimethoxycinnamic acid with production of methanol. | when grown on 3,4,5-trimethoxycinnamic acid, a strain of pseudomonas putida oxidized this compound and also 3,4,5-trimethoxybenzoic, 3,5-dimethoxy-4-hydroxybenzoic (syringic), and 3,4-dihydroxy-5-methoxybenzoic (3-o-methylgallic) acids, but 3,5-dimethoxy-4-hydroxycinnamic and other acids bearing structural resemblances to the growth substrate were oxidized only slowly. these results indicate that two carbon atoms of the side chain of 3,4,5-trimethoxycinnamate were released before oxidative demet ... | 1981 | 7263612 |
| plasmid- and chromosome-mediated dissimilation of naphthalene and salicylate in pseudomonas putida pmd-1. | pseudomonas putida pmd-1 dissimilates naphthalene (nah), salicylate (sal), and benzoate (ben) via catechol which is metabolized through the meta (or alpha-keto acid) pathway. the ability to utilize salicylate but not naphthalene was transferred from p. putida pmd-1 to several pseudomonas species. agarose gel electrophoresis of deoxyribonucleic acid (dna) from pmd-1 and sal+ exconjugants indicated that a plasmid (pmwd-1) of 110 megadaltons is correlated with the sal+ phenotype; restriction enzyme ... | 1981 | 7275935 |
| metabolism of allylglycine and cis-crotylglycine by pseudomonas putida (arvilla) mt-2 harboring a tol plasmid. | spontaneous mutants which acquired the ability to utilize d-allylglycine (d-2-amino-4-pentenoic acid) and dl-cis-crotylglycine (dl-2-amino-cis-4-hexenoic acid) but not l-allylglycine or dl-trans-crotylglycine could be readily isolated from pseudomonas putida mt-2 (pam1). derivative strains of pam1 putatively cured of the tol (pwwo) plasmid were incapable of forming mutants able to utilize the amino acids for growth; however, this ability could be regained by conjugative transfer of the tol (pwwo ... | 1981 | 7287632 |
| regulation of phenol degradation in pseudomonas putida. | in order to characterize the ability of pseudomonas putida (trevisan 1889) migula 1895 strain h to degrade various mono- and diphenolic aromatic compounds, respiratory activities towards phenol, catechol, and the cresol isomers were determined. the following rates of oxygen uptake (qo2) were obtained with resting phenol-grown cells: phenol -- 229, o-cresol -- 231, m-cresol -- 43, p-cresol -- 200, catechol -- 262. all these compounds were oxidized by a two-phase-kinetics, the first phase is chara ... | 1981 | 7293241 |
| magnetic and natural circular dichroism spectra of cytochgromes p-450(11) beta and p-450scc purified from bovine adrenal cortex. | magnetic (mcd) and natural circular dichroism (cd) spectra various complexes of cytochrome p-450(11) beta (p-450(11) beta) and cytochrome p-450scc (p-450scc) from bovine adrenal cortex were measured from 250 nm to 700 nm. mcd and cd spectral contours of cytochromes p-450(11) beta and p-450scc in the soret and visible regions were, as a whole, analogous to those of cytochromes p-450 from rabbit liver microsomes and also from pseudomonas putida in their high-spin ferric, high-spin ferrous and ferr ... | 1981 | 7295771 |
| purification of a branched-chain keto acid dehydrogenase from pseudomonas putida. | we purified branched-chain keto acid dehydrogenase to a specific activity of 10 mumol/min per mg of protein from pseudomonas putida grown on valine. the purified enzyme was active with 2-ketoisovalerate, 2-ketoisocaproate, and 2-keto-3-methylvalerate in a ratio of 1.0:0.8:0.7 but showed no activity with either pyruvate or 2-ketoglutarate. there were four polypeptides in the purified enzyme (molecular weights, 49,000, 46,000, 39,000, and 37,000). the purified enzyme was deficient in the specific ... | 1981 | 7298579 |
| 2,3-dihydroxybenzoate pathway in pseudomonas putida. 1h n.m.r. study on the ring-cleavage site. | 1. ring cleavage of 2,3-dihydroxybenzoate by cell-free extracts of pseudomonas putida leads to 2-hydroxy-6-oxo-(2z,4e)-hexa-2,4-dienoic acid and co2. 2. the 1h n.m.r. spectrum of the ring-fission product obtained in a 2h2o solution suggests that the extra-diol cleavage occurs between c-3 and c-4. | 1981 | 7306005 |
| mössbauer spectroscopic studies of the terminal dioxygenase protein of benzene dioxygenase from pseudomonas putida. | mössbauer spectra obtained from the terminal dioxygenase protein of the benzene dioxygenase system from pseudomonas putida show that it contains [2fe--2s] centres similar to those of the two-iron plant-type ferredoxins. in the oxidized form the two iron atoms within the centre are high-spin ferric but with considerable inequivalence. in the reduced form the centre contains one extra electron, and this is localized on one of the iron atoms, which becomes high-spin ferrous. | 1981 | 7306045 |
| the reaction of oxygen with protocatechuate 3,4-dioxygenase from pseudomonas putida. characterization of a new oxygenated intermediate. | the reactions of protocatechuate dioxygenase (protocatechuate:oxygen 3,4-oxidoreductase, ec 1.13.11.3) with substrates and oxygen have been studied at 4 degrees c using rapid kinetic techniques. in this study, two oxygenated intermediates were kinetically and spectrally characterized. the rate of oxygen addition to the enzyme-substrate complex was determined to be 5 x 10(5) m-1 s-1. this oxygenated complex rapidly converts (450 s-1) to another spectrally identifiable compound which then breaks d ... | 1981 | 7309730 |
| participation of the beta-ketoadipate transport system in chemotaxis. | beta-ketoadipate serves as a chemoattractant for pseudomonas putida. the chemotactic response is inducible, and a regulatory mutant strain that forms the beta-ketoadipate transport system at high levels exhibits a heightened chemotactic response to beta-ketoadipate. adipate and succinate, compounds that interact with the transport system, inhibit chemotaxis toward beta-ketoadipate. some, but not all, mutants that fail to respond chemotactically to beta-ketoadipate lack the beta-ketoadipate trans ... | 1981 | 7320700 |
| [evaluation of biological activity of l-methionidase from pseudomonas putida ac-75 on the basis of in vitro studies]. | 1981 | 7342702 | |
| digestion of algin by pseudomonas maltophilia and pseudomonas putida. | pseudomonas maltophilia and pseudomonas putida were identified as alginolytic species. two media used for demonstrating alginolytic activity are described. the applied aspects of the ability of these two species to digest algin are discussed. | 1980 | 7356324 |
| the purification and characterization of delta 5-3-ketosteroid isomerase from pseudomonas putida, a cysteine-containing isomerase. | a delta 5-3-ketosteroid isomerase (ec 5.3.3.1) has been isolated from pseudomonas putida biotype b and purified to homogeneity. this previously undescribed steroid isomerase resembles that isolated from pseudomonas testosteroni (talalay, p., and wang, v.s. (1955) biochim. biophys. acta 18, 300-301). the enzyme is induced by various steroids, has a subunit molecular weight of 13,750 +/- 250, a pi of 4.8 +/- 0.1 has a specific activity of 40,000 units/mg, using 5-androstene-3,17-dione as the subst ... | 1980 | 7358699 |
| kinetics of the isomerization of 5-androsten-3,17-dione catalyzed by delta 5-3-ketosteroid isomerase from pseudomonas putida. | 1980 | 7358700 | |
| active site-directed photoinactivation of delta 5-3-ketosteroid isomerase from pseudomonas putida dependent on 1,4,6-androstatrien-3-one-17 beta-ol. | delta 5-3-ketosteroid isomerase from pseudomonas putida is subject to photoinactivation by light of wavelengths greater than 300 nm, specifically in the presence of the competitive inhibitor, 1,4,6-androstatrien-3-one-17 beta-ol (teo). in the absence of this steroid or in the presence of the nonchromophoric steroidal competitive inhibitor, deoxycholate, the enzyme activity is essentially unaffected by irradiation. deoxycholate protects the enzyme from the teo-dependent reaction to a degree which ... | 1980 | 7358701 |
| purification and characterization of an oxygenase component in benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1. | the benzoate 1,2-dioxygenase system of pseudomonas arvilla consists of two proteins, an nadh-cytochrome c reductase and an oxygenase. the oxygenase component was purified to apparent homogeneity by the criteria of polyacrylamide gel electrophoresis from benzoate-induced cells of p. arvilla. the molecular weight of the enzyme was determined to be 273,000 by sedimentation equilibrium analysis, 280,000 by electrophoresis on polyacrylamide gels of different concentrations, and 270,000 by sepharose c ... | 1980 | 7372624 |
| energetics of myo-inositol transport in pseudomonas putida. | the effects of specific inhibitors on the high-affinity transport system of cyclitols and on the respiration of pseudomonas putida shows that the transport activity is dependent on high energy phosphate bond. | 1980 | 7379940 |
| hybrid pathway for chlorobenzoate metabolism in pseudomonas sp. b13 derivatives. | derivatives of pseudomonas sp. b13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the tol plasmid of pseudomonas putida mt-2 were studied. the utilization of these substrates, a property not shared by the parent strains, was shown to depend upon the combined activities of enzymes from the donor and from the recipient. during growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate, predominantly the toluate 1,2-deo ... | 1980 | 7380800 |
| production of methanol from aromatic acids by pseudomonas putida. | when grown at the expense of 3,4,5-trimethoxybenzoic acid, a strain of pseudomonas putida oxidized this compound and also 3,5-dimethoxy-4-hydroxybenzoic (syringic) and 3,4-dihydroxy-5-methoxybenzoic (3-o-methylgallic) acids; but other hydroxy- or methoxy-benzoic acids were oxidized slowly or not at all. radioactivity appeared exclusively in carbon dioxide when cells were incubated with [4-methoxyl-14c]trimethoxybenzoic acid, but was found mainly in methanol when[methoxyl-14c]3-o-methylgallic aci ... | 1980 | 7380811 |
| a simple method for the preparation of d-alpha-aminoadipic acid. | high quantity (1 g and more) of racemically and chromatographically pure d-alpha-aminoadipic acid was prepared by selective metabolism of the l-isomer of the commercially available dl-alpha-aminoadipate by pseudomonas putida. the overall yield of this preparation averaged 40%. the final product has [a]25d value of -25 degrees. this procedure can be useful in the synthesis of high purity d-alpha-amino-adipate, a compound shown recently to be a useful tool in the study of neurotransmission mechani ... | 1980 | 7383979 |
| repetitions in the nh2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from pseudomonas putida. | muconolactone delta-isomerase (ec 5.3.3.4) and beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) mediate consecutive reactions in the beta-ketoadipate pathway of bacteria. an earlier investigation (yeh, w.k., davis, g., fletcher, p., and ornston, l.n. (1978) j. biol. chem. 253, 4920-4923) revealed that the respective nh2-terminal amino acid sequences of pseudomonas putida muconolactone isomerase and acinetobacter calcoaceticus beta-ketoadipate enol-lactone hydrolase ii are evolutionarily hom ... | 1980 | 7391022 |
| homologies in the nh2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases. | gamma-carboxymuconolactone decarobxylase (ec 4.1.1.44) and muconolactone isomerase (ec 5.3.3.4) mediate chemically analogous reactions in bacteria. the enzymes are inducible, and different metabolites trigger the respective syntheses of the decarboxylases in acinetobacter calcoaceticus and pseudomonas putida. the decarobxylases share similar oligomeric structures in which identical subunits of about 13,300 daltons appear to be self-associated into hexamers. identical residues are found in 18 of ... | 1980 | 7391024 |
| [microbiological transformation of quinoline by pseudomonas putida bacteria]. | 1980 | 7402102 | |
| [electrokinetic properties of arthobacter siderocapsulatus]. | the electro-kinetic properties of arthrobacter siderocapsulatus were determined using microelectrophoresis in alternating electric field. the surface of bacterial cells was shown to bear positive charge within a wide range of ph. the positive charge of the cells should be attributed apparently to the presence of mucous capsules containing manganese dioxide since the growth of the bacterium is known to involve oxidation of manganese or ferrous iron and accumulation of their hydroxides in the caps ... | 1980 | 7402120 |
| degradation of (-)-ephedrine by pseudomonas putida. detection of (-)-ephedrine: nad+-oxidoreductase from arthrobacter globiformis. | a bacterium utilizing the alkaloid (-)-ephedrine as its sole source of carbon was isolated by an enrichment-culture technique from soil supplemented with 4-benzoyl-1,3-oxazolidinon-(2). the bacterium was indentified as pseudomonas putida by morphological and physiological studies. the following metabolites were isolated from the culture fluid: methylamine, formaldehyde, methylbenzoylcarbinol (2-hydroxy-1-oxo-1 phenylpropane), benzoid acid, pyrocatechol and cis, cis-muconic acid. a pathway for th ... | 1980 | 7405363 |
| cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from pseudomonas ovalis. | the cd-, cr-, and mn-substituted enzymes of iron-superoxide dismutase from pseudomonas ovalis were prepared from the apoenzyme by using the alkaline treatment method described before (1) with a slight modification. the cd-substituted enzyme had 1.16 g atoms of cd per mol of enzyme and no visible absorption. the cr-substituted enzyme had 1.27 g atoms of cr per mol of enzyme and had absorption maxima at 530 nm and 670 nm with a shoulder around 370 nm. the mn-substituted enzyme had 1.27 g atoms of ... | 1980 | 7410333 |
| enzymological aspects of caffeine demethylation and formaldehyde oxidation by pseudomonas putida c1. | 1) the enzymatic demethylation of caffeine (1,3,7-trimethylxanthine) by pseudomonas putida c1 was investigated; an inducible enzyme system has been observed. this enzyme shows an optimum ph of about 6.0, and the optimum temperature is in the range of 22-24 degrees c. the enzyme is absolutely dependent on nadh or nadph as a cosubstrate and is activated by co2+. 2) the formaldehyde generated by the demethylation of caffeine is oxidized by an nad-dependent formaldehyde dehydrogenase, which is indep ... | 1980 | 7461603 |
| [frequency of clinical isolation of glucose non-fermentative gram-negative rods and their susceptibilities to antibacterial agents]. | a comparison was made for frequencies of isolation o glucose non-fermentative gram-negative rods ((g)nf-gnr) from clinical specimens during a period from july, 1986 to june, 1987 (the first period) and that from january, 1994 to december, 1994 (the second period). also, minimum inhibitory concentrations of principal drugs were determined against these isolates. the obtained results are summarized as follows: 1. thirty four (34) species of (g)nf-gnr were found from 35,200 clinical specimens in th ... | 1995 | 7474336 |
| multiple outer membrane receptors for uptake of ferric pseudobactins in pseudomonas putida wcs358. | under iron limitation pseudomonas putida wcs358 produces a fluorescent siderophore, pseudobactin 358, which, after complexing iron, is transported back into the cell via the specific outer membrane receptor pupa. in addition, this strain has the capacity to take up iron via a large variety of siderophores produced by other fluorescent pseudomonads. putative receptor genes for such siderophores were identified in the chromosome of strain wcs358 by pcr using primers matching two domains conserved ... | 1995 | 7476877 |
| transformations of morphine alkaloids by pseudomonas putida m10. | the oxidation of morphine by washed-cell incubations of pseudomonas putida m10 gave rise to a large number of transformation products including hydromorphone (dihydromorphinone), 14 beta-hydroxymorphine, 14 beta-hydroxymorphinone, and dihydromorphine. similarly, in incubations with oxymorphone (14 beta-hydroxydihydromorphinone) as substrate, the major transformation product was identified as oxymorphol (14 beta-hydroxydihydromorphine). the identities of all these biological products were confirm ... | 1995 | 7487001 |
| role of competition for inorganic nutrients in the biodegradation of mixtures of substrates. | a study was conducted to determine whether competition for inorganic nutrients affects the biodegradation of mixtures of substrates. little benzylamine was mineralized by pseudomonas putida in solutions with no added p, but the substrate was degraded if the medium contained 100 nm p. the enhancement by p addition did not occur if the medium also contained caprolactam and a caprolactam-utilizing strain of pseudomonas aeruginosa. the suppression by the second bacterium was overcome by a higher p c ... | 1995 | 7487018 |
| construction and behavior of biologically contained bacteria for environmental applications in bioremediation. | the survival of microorganisms can be predicted through the use of active biological containment systems. we have constructed contained pseudomonas putida strains that degrade alkylbenzoates. the modified strain carries a fusion of the plac promoter to the gef gene, which encodes a killing protein. expression from plac is controlled through a regulatory cascade, so that plac is switched on or off by the absence or presence of alkylbenzoates, respectively. similar uncontained strains were also co ... | 1995 | 7487030 |
| production of a form-specific, inhibitory antibody against rat cytochrome p450 2b1 using a synthetic peptide antigen against a putative substrate binding site. | rat cytochrome p450 2b1 antipeptide antibodies were produced by immunizing rabbits with a synthetic peptide antigen. the anti-cyp2b1 igg obtained did not cross-react with cyp2b2, which has 97% identity in primary sequence of cyp2b1. this result demonstrates that a difference of 2 amino acid residues among 12 is sufficient to produce a form-specific antibody. the cyp2b1 antipeptide igg inhibited pentoxyresorufin o-dealkylase activity of microsomes obtained from phenobarbital-treated rats in a dos ... | 1995 | 7488175 |
| pollution of modern metalworking fluids containing biocides by pathogenic bacteria in france. reexamination of chemical treatments accuracy. | pollution by pathogenic bacteria was examined in 150 french metalworking fluid samples. gram-negative micro-organisms such as salmonella spp., shigella spp., and vibrio spp. as well as gram-positive cocci were never isolated. nevertheless opportunistic pathogens such as pseudomonas aeruginosa and klebsiella pneumoniae still contaminated these fluids with an isolation frequency of 17% of samples for each. these two micro-organisms failed to grow or even survive in vitro in sterile cutting fluids ... | 1995 | 7489767 |
| dissection of the core and auxiliary sequences in the vegetative replication origin of promiscuous plasmid rk2. | the vegetative replication origin (oriv) of promiscuous incp plasmid rk2 can function in many gram-negative bacterial species when supplied with the plasmid-encoded replication protein trfa and host-encoded replication proteins including dnaa. nine trfa binding sites (iterons) are known, and also two dnaa binding sites, box 1, between trfa iterons 4 and 5, and box 2, downstream of repeat 9. the deletion analysis presented here shows that the core oriv requires dnaa box 1 for function in escheric ... | 1995 | 7500337 |
| the refined x-ray structure of muconate lactonizing enzyme from pseudomonas putida prs2000 at 1.85 a resolution. | we report here the refined x-ray crystal structure of muconate lactonizing enzyme (mle) from pseudomonas putida prs2000 at a resolution of 1.85 a with an r-factor of 16.8%. an enzyme from the beta-ketoadipate pathway, mle catalyses the conversion of cis,cis-muconate to muconolactone. it is a homo-octamer, one monomer consisting of 373 amino acid residues. mle has two large domains and a c-terminal subdomain: an alpha + beta domain, an alpha beta-barrel domain and a c-terminal meandering subdomai ... | 1995 | 7500361 |
| antimicrobial activity and biosynthesis of indole antibiotics produced by xenorhabdus nematophilus. | we have investigated the mechanism of action and physiology of production of the indole derivative antibiotics produced by the nematode-associated, entomopathogenic bacterium xenorhabdus nematophilus. maximum antibiotic concentration was reached during the late stationary phase of growth, and the antibiotic yield was appreciably enhanced by supplementation with tryptophan. antibiotic biosynthesis apparently involved the removal of the side-chain carboxyl (c-1) carbon of tryptophan. the c-3 methy ... | 1993 | 7510325 |
| nucleotide sequence and functional analysis of the genes encoding 2,4,5-trichlorophenoxyacetic acid oxygenase in pseudomonas cepacia ac1100. | pseudomonas cepacia ac1100 is able to use the chlorinated aromatic compound 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) as the sole source of carbon and energy. one of the early steps in this pathway is the conversion of 2,4,5-t to 2,4,5-trichlorophenol (2,4,5-tcp). 2,4,5-tcp accumulates in the culture medium when ac1100 is grown in the presence of 2,4,5-t. a dna region from the ac1100 genome has been subcloned as a 2.7-kb ssti-xbai dna fragment, which on transfer to pseudomonas aeruginosa pao1 ... | 1994 | 7527626 |
| cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs. | the two-component nonheme iron dioxygenase system 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs catalyzes the double hydroxylation of 2-halobenzoates with concomitant release of halogenide and carbon dioxide, yielding catechol. the gene cluster encoding this enzyme, cbdabc, was localized on a 70-kbp conjugative plasmid designated pbah1. the nucleotide sequences of cbdabc and flanking regions were determined. in the deduced amino acid sequence of the large subunit of the terminal o ... | 1995 | 7530709 |
| identification of a membrane protein and a truncated lysr-type regulator associated with the toluene degradation pathway in pseudomonas putida f1. | a 3 kb dna region upstream of the toluene degradation (tod) genes, todfc1c2badegih, in pseudomonas putida f1 (ppf1) was sequenced. two divergently arranged open reading frames, todr and todx, were identified. a toluene-inducible promoter was localized in front of todx, and the transcription start point was mapped. this promoter is probably responsible for the expression of all tod structural genes. todx was found to be a membrane protein. its predicted amino acid sequence (453 residues; m(r) 48, ... | 1995 | 7535376 |
| self-induced bacteremia. case report. | we describe a patient with perforated appendicitis who postoperatively suffered repeated episodes of shaking chills and temperature spikes. initial blood cultures yielded growth of flavobacterium meningosepticum, pseudomonas putida and pseudomonas paucimobilis, and succeeding blood cultures growth of pseudomonas acidoverans. these bacteria in combination led to a suspicion of self-inoculation of contaminated water through an intravenous catheter. antibiotic treatment had no effect on the symptom ... | 1995 | 7546651 |
| sequence motifs in a flagellin of pseudomonas putida. | 1995 | 7551017 | |
| quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from comamonas testosteroni 63. the first two enzymes in quinoline and 3-methylquinoline degradation. | the enzymes catalysing the first two steps of quinoline and 3-methylquinoline degradation by comamonas testosteroni 63 were investigated. quinoline 2-oxidoreductase, which catalyses the hydroxylation of (3-methyl-)quinoline to (3-methyl-)2-oxo-1,2-dihydroquinoline, was purified to apparent homogeneity. the native enzyme, with a molecular mass of 360 kda, is composed of three non-identical subunits (87, 32, and 22 kda), occurring in a ratio of 1.16:1:0.83. containing fad, molybdenum, iron, and ac ... | 1995 | 7556204 |
| cloning, characterization and expression of the gene encoding cytochrome p-450sca-2 from streptomyces carbophilus involved in production of pravastatin, a specific hmg-coa reductase inhibitor. | pravastatin, a drug for treating hypercholesterolemia, is produced by hydroxylation of ml-236b-na in streptomyces carbophilus (sc) catalyzed by the cytochrome p-450sca (cytp-450sca) monooxygenase system. the gene (cytp-450sca-2) encoding cytp-450sca-2 was cloned from sc. the gene had an open reading frame of 1233 bp, encoding a 410-amino-acid protein. the partial sequencing of the purified cytp-450sca-2 revealed that the n-terminal met had been removed. cytp-450sca-2 contained the heme-binding h ... | 1995 | 7557483 |
| activation of the transcriptional regulator xylr of pseudomonas putida by release of repression between functional domains. | in the presence of toluene, xylenes and other structural analogues, the regulatory protein xylr, of the family of transcriptional regulators which act in concert with the sigma 54 factor, activate the promoter pu of the tol (toluene degradation) plasmid pwwo of pseudomonas putida. amino acid changes val-219-asp and ala-220-pro, introducing a proline kink at the hinge region between the n-terminal a domain and the central portion of xylr, resulted in a semi-constitutive phenotype which mimicked t ... | 1995 | 7565083 |
| characterization of the pilf-pild pilus-assembly locus of neisseria gonorrhoeae. | expression of type iv pili by the bacterial pathogen neisseria gonorrhoeae appears to be essential for colonization of the human host. several n. gonorrhoeae gene products have been recently identified which bear homology to proteins involved in pilus assembly and protein export in other bacterial systems. we report here the isolation and characterization of transposon insertion mutants in n. gonorrhoeae whose phenotypes indicate that the n. gonorrhoeae pilf and pild gene products are required f ... | 1995 | 7565116 |
| roa307, a protein encoded on coxiella burnetii plasmid qph1, shows homology to proteins encoded in the replication origin region of bacterial chromosomes. | tnphoa mutagenesis identified an open reading frame, roa307, immediately upstream of the partition locus qsopab on the coxiella burnetii plasmid qph1. the protein sequence deduced from roa307 displayed homology to orf290 of pseudomonas putida, orf283 and orf282 (spooj) of bacillus subtilis-hypothetical products of genes in the chromosomal replication origin region. expression of roa307 was demonstrated by phoa activity of an roa307-phoa fusion. | 1995 | 7565613 |
| identification of exopolysaccharides produced by fluorescent pseudomonads associated with commercial mushroom (agaricus bisporus) production. | the acidic exopolysaccharides (epss) from 63 strains of mushroom production-associated fluorescent pseudomonads which were mucoid on pseudomonas agar f medium (paf) were isolated, partially purified, and characterized. the strains were originally isolated from discolored lesion which developed postharvest on mushroom (agaricus bisporus) caps or from commercial lots of mushroom casing medium. an acidic galactoglucan, previously named marginalan, was produced by mucoid strains of the saprophyte ps ... | 1995 | 7574589 |
| possible regulatory role for nonaromatic carbon sources in styrene degradation by pseudomonas putida ca-3. | styrene metabolism in styrene-degrading pseudomonas putida ca-3 cells has been shown to proceed via styrene oxide, phenylacetaldehyde, and phenylacetic acid. the initial step in styrene degradation by strain ca-3 is oxygen-dependent epoxidation of styrene to styrene oxide, which is subsequently isomerized to phenylacetaldehyde. phenylacetaldehyde is then oxidized to phenylacetic acid. styrene, styrene oxide, and phenylacetaldehyde induce the enzymes involved in the degradation of styrene to phen ... | 1995 | 7574594 |
| expression vectors for the use of eukaryotic luciferases as bacterial markers with different colors of luminescence. | an easy way to identify microorganisms is to provide them with gene markers that confer a unique phenotype. several genetic constructions were developed to use eukaryotic luciferase genes for bacterial tagging. the firefly and click bettle luciferase genes, luc and lucor, respectively, were cloned under constitutive control and regulated control from different transcriptional units driven by p1, lambda pr, and ptrc promoters. comparison of the expression of each gene in escherichia coli cells fr ... | 1995 | 7574604 |
| nucleotide sequence analysis of genes encoding a toluene/benzene-2-monooxygenase from pseudomonas sp. strain js150. | it was previously shown by others that pseudomonas sp. strain js150 metabolizes benzene and alkyl- and chloro-substituted benzenes by using dioxygenase-initiated pathways coupled with multiple downstream metabolic pathways to accommodate catechol metabolism. by cloning genes encoding benzene-degradative enzymes, we found that strain js150 also carries genes for a toluene/benzene-2-monooxygenase. the gene cluster encoding a 2-monooxygenase and its cognate regulator was cloned from a plasmid carri ... | 1995 | 7574644 |
| quantification of the effect of substrate concentration on the conjugal transfer rate of the tol plasmid in short-term batch mating experiments. | batch mating experiments with pseudomonas putida paw 1 (tol) as a donor and pseudomonas aeruginosa pao 1162 as a recipient strain were performed to quantify the effect of the substrate concentration in the mating medium on the observed plasmid transfer rate coefficient. the impact of the substrate concentration in the mating medium was highly correlated with the growth history of the donor strain. when the donor strain was harvested in exponential growth phase, no impact was observed; when the d ... | 1995 | 7576502 |
| the 2fe2s centres of the 2-oxo-1,2-dihydroquinoline 8-monooxygenase from pseudomonas putida 86 studied by epr spectroscopy. | the 2-oxo-1,2-dihydroquinoline 8-monooxygenase from pseudomonas putida 86 comprises two components with four redox active sites necessary for activity. we present an epr characterization of the iron-sulfur centres in the purified reductase and oxygenase component of this novel enzyme system. the oxygenase component was identified as a rieske [2fe2s] protein on the basis of its characteristic epr spectrum with gz,y,x = 2.01, 1.91, 1.76 and gav = 1.893. the reductase component, an iron-sulfur flav ... | 1995 | 7578219 |
| [modification of the ortho-route in pseudomonas putida strain 87: purification and properties of dienlactone hydrolase]. | dienelactone hydrolase (dlh) was purified to electrophoretic homogeneity from the biomass of the pseudomonas putida strain 87 grown on 3-chlorobenzoate. the specific activity of the purified enzyme is 50.5 u./mg of protein. the enzyme is a monomer with a molecular mass of 22 kda, has a ph optimum at 7.6 and is active within a broad temperature range (20-45 degrees c). the enzyme activity is inhibited by pcmb (which requires up to two equivalents of the reagent) but not by edta. the vmax values o ... | 1995 | 7578578 |
| use of haloacetate dehalogenase genes as selection markers for escherichia coli and pseudomonas vectors. | the haloacetate dehalogenase gene, dehh2, cloned from moraxella sp. strain b could be used a selection marker gene for vectors in escherichia coli and pseudomonas putida. haloacetates, especially iodoacetate, inhibit the growth of some microorganisms. the dehh2 gene introduced into the cells conferred iodoacetate resistance on them. therefore, e. coli and p. putida transformed with vectors marked with dehh2 could be easily selected on plates containing iodoacetate. | 1995 | 7579995 |
| the nucleotide sequence of a transposable haloalkanoic acid dehalogenase regulatory gene (dehri) from pseudomonas putida strain pp3 and its relationship with sigma 54-dependent activators. | the mobile genetic element, deh found in pseudomonas putida pp3 carries a 2-haloalkanoic acid dehalogenase structural gene, dehi, and its associated regulatory gene, dehri. the nucleotide sequence of dehri was determined. the gene had an open reading frame putatively encoding for a 64 kda protein containing 571 amino acid residues. the protein was similar to previously published sequences of several other sigma 54-dependent activator proteins. amino acid sequence analysis showed that the deduced ... | 1995 | 7579999 |
| the use of two-dimensional gradient plates to investigate the range of conditions under which conjugal plasmid transfer occurs. | gel-stabilized two-dimensional gradient plates were used to study the effects of ph, salt concentration and temperature on the conjugal transfer of plasmid rp4 between strains of escherichia coli and pseudomonas putida. the combinations of ph and salt concentration that permitted conjugation were mapped as a two-dimensional growth area occupied by transconjugants following conjugation. this conjugation domain was less extensive than the areas that supported growth of the parental strains, and sh ... | 1995 | 7582032 |
| extraction and purification of microbial dna from petroleum-contaminated soils and detection of low numbers of toluene, octane and pesticide degraders by multiplex polymerase chain reaction and southern analysis. | we investigated the use of multiplex polymerase chain reaction (pcr) techniques coupled with southern analysis to detect xenobiotic-degrading organisms that had been added to three soils. two soils highly contaminated with petroleum hydrocarbons and a less contaminated control soil were amended with tenfold dilutions of pseudomonas putida mt-2 (pww0), p. oleovorans (oct), and alcaligenes eutrophus jmp134 (pjp4), or, for controls, phosphate buffer alone. total dna was then isolated from the soils ... | 1995 | 7582166 |
| low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium pseudomonas putida gr12-2. | the plant growth promoting rhizobacterium pseudomonas putida gr12-2 was originally isolated from the rhizosphere of plants growing in the canadian high arctic. here we report that this bacterium was able to grow and promote root elongation of both spring and winter canola at 5 degrees c, a temperature at which only a relatively small number of bacteria are able to proliferate and function. in addition, the bacterium survived exposure to freezing temperatures, i.e., -20 and -50 degrees c. in an e ... | 1995 | 7585354 |
| sequence and expression of the bpdc1c2bade genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by rhodococcus sp. m5. | the nucleotide (nt) sequence of the bpdc1c2bade genes which encode the first three enzymes in the biphenyl (bp) degradation pathway of gram+ rhodococcus sp. m5 (formerly arthrobacter m5) was determined. except for the ferredoxin component (bpdb) of the initial bp dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (todc1c2bade) present in the toluene-degrader, pseudomonas putida f1, than those of three bp ... | 1995 | 7590299 |
| desaturation and oxygenation of 1,2-dihydronaphthalene by toluene and naphthalene dioxygenase. | bacterial strains expressing toluene and naphthalene dioxygenase were used to examine the sequence of reactions involved in the oxidation of 1,2-dihydronaphthalene. toluene dioxygenase of pseudomonas putida f39/d oxidizes 1,2-dihydronaphthalene to (+)-cis-(1s,2r)-dihydroxy-1,2,3,4-tetrahydronaphthalene, (+)-(1r)-hydroxy-1,2-dihydronaphthalene, and (+)-cis-(1r,2s)-dihydroxy-1,2-dihydronaphthalene. in contrast, naphthalene dioxygenase of pseudomonas sp. strain ncib 9816/11 oxidizes 1,2-dihydronaph ... | 1995 | 7592326 |
| formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids. | the p-cumate-degrading strain pseudomonas putida f1 and the m- and p-toluate-degrading strain p. putida mt-2 transform indole-2-carboxylate and indole-3-carboxylate to colored products identified here as indigo, indirubin, and isatin. a mechanism by which these products could be formed spontaneously following dioxygenase-catalyzed dihydroxylation of the indolecarboxylates is proposed. indolecarboxylates were employed as chromogenic substrates for identifying recombinant bacteria carrying genes e ... | 1995 | 7592495 |
| production and application of monoclonal antibodies specific to pyrroloquinoline quinone. | we produced five monoclonal antibodies (mabs 1, 2, 6, 7, and 9) that are specific to pyrroloquinoline quinone (pqq). pqq-conjugated hemocyanin was used for the immunization of mice and the hybridomas were selected using pqq-conjugated bsa in an enzyme-linked immunosorbent assay. mabs 2 and 9 were of the igg1 isotype. both could recognize free pqq, the former probably at the o-quinone and the latter at the opposite side of the molecule. they did not bind with trihydroxyphenylalanine, dihydroxyphe ... | 1995 | 7592546 |
| differential dna bending introduced by the pseudomonas putida lysr-type regulator, catr, at the plasmid-borne pheba and chromosomal catbc promoters. | the plasmid-borne pheba operon of pseudomonas putida strain paw85 allows growth of the host cells on phenol. the promoter of this operon is activated by the chromosomally encoded lysr-type regulator catr, in the presence of the inducer cis,cis-muconate. cis,cis-muconate is an intermediate of catechol degradation by the chromosomally encoded ortho or beta-ketoadipate pathway. the catbc operon encodes two enzymes of the beta-ketoadipate pathway and also requires catr and cis,cis-muconate for its e ... | 1995 | 7596284 |
| the sigma 54-dependent promoter ps of the tol plasmid of pseudomonas putida requires hu for transcriptional activation in vivo by xylr. | in the presence of toluene and xylenes, the sigma 54-dependent ps promoter of the tol (toluene biodegradation) plasmid pww0 of pseudomonas putida is activated at a distance by the xylr protein, of the ntrc family of transcriptional regulators. since contacts between xylr bound to upstream activating sites and the rna polymerase require the looping out of the intervening dna segment, the intrinsic curvature, the bendability of the corresponding sequence, and the spatial effects of protein-induced ... | 1995 | 7601841 |
| evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway. | the maleylacetate reductase from pseudomonas sp. strain b13 functioning in the modified ortho pathway was purified and digested with trypsin. the polypeptides separated by high-performance liquid chromatography were sequenced. alignments with the polypeptides predicted from the tfdf and tcbf genes located on plasmids pjp4 of the 2,4-dichlorophenoxyacetate-degrading alcaligenes eutrophus jmp134 and pp51 of the 1,2,4-trichlorobenzene-degrading pseudomonas sp. strain p51 as well as polypeptides pre ... | 1995 | 7601858 |
| autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in pseudomonas aeruginosa. | the opportunistic human pathogen pseudomonas aeruginosa produces a variety of virulence factors, including exotoxin a, elastase, alkaline protease, alginate, phospholipases, and extracellular rhamnolipids. the previously characterized rhlabr gene cluster encodes a regulatory protein (rhlr) and a rhamnosyltransferase (rhlab), both of which are required for rhamnolipid synthesis. another gene, rhii, has now been identified downstream of the rhlabr gene cluster. the putative rhli protein shares sig ... | 1995 | 7604006 |
| cloning of the ppu21im gene using a in vivo selection method. | a genetic system enabling the in vivo selection of genes encoding the dna-modifying enzymes was developed. a gene library is transformed into a strain harboring the restriction-modification (r-m) system which a recognition sequence is a subset of the target sequence of the dna methyltransferase (mtase) to be cloned. if the residing mtase is temperature sensitive, the inability of transformants to grow at 42 degrees c provides a simple and convenient procedure for the isolation of new mtase-encod ... | 1995 | 7607525 |
| isolation and expansion of the catabolic potential of a pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons. | pseudomonas putida dot-t1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole c source. the strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log p(ow) (octanol/water partition coefficient) was higher than 2.3. solvent tolerance was inducible, as bacteria grown in the absence of toluene required an adaptation period before growth restarted. mg2+ ions in the culture medium improved solvent tolerance. elect ... | 1995 | 7608060 |
| biosensing of benzene derivatives in the environment by luminescent escherichia coli. | sensitive and convenient biosensing of environmental pollutants has been developed by fusing a gene of firefly luciferase to the tol plasmid. tol plasmid of pseudomonas putida encodes a series of enzymes for degradation of benzene and its derivatives. the expression of these enzymes is controlled with the regulating proteins xylr and xyls, whose promoters are activated in the presence of aromatic compounds. the structural gene of firefly luciferase, as a reporter enzyme, was inserted under the c ... | 1995 | 7612210 |
| cyclodextrin-enhanced degradation of toluene and p-toluic acid by pseudomonas putida. | degradation of an immiscible aromatic solvent, toluene, and a water-soluble aromatic compound, p-toluic acid, by a pseudomonas putida strain in the presence of beta-cyclodextrin (beta-cd) was investigated. the ability of cds to interact with hydrophobic organics and form inclusion compounds was exploited in this study to remove or alleviate the toxicities of substrates and consequently to enable or enhance degradation. liquid toluene was found to be highly toxic to p. putida. however, this phase ... | 1995 | 7618884 |
| simultaneous chromium reduction and phenol degradation in a coculture of escherichia coli atcc 33456 and pseudomonas putida dmp-1. | in a defined coculture of a cr(vi) reducer, escherichia coli atcc 33456, and a phenol degrader, pseudomonas putida dmp-1, simultaneous reduction of cr(vi) and degradation of phenol was observed. when cr(vi) was present in the coculture, quantitative transformation of cr(vi) into cr(iii) proceeded with simultaneous degradation of phenol. cr(vi) reduction was correlated to phenol degradation in the coculture as demonstrated by a regression analysis of the cumulative cr(vi) reduction and the cumula ... | 1995 | 7618887 |
| iron-ligand structure and iron redox property of nitric oxide reductase cytochrome p450nor from fusarium oxysporum: relevance to its no reduction activity. | we studied the nitric oxide reductase, cytochrome p450nor, purified from a denitrifying fungus fusarium oxysporum with electron paramagnetic resonance spectral and redox potential measurements. the epr spectral features of p450nor in the ferric resting, the ferric cyanide-bound, and the ferrous no-bound forms were the same as the corresponding ones of other general p450s such as pseudomonas putida p450cam. in contrast, the metyrapone complex of ferric p450nor gave an epr spectrum with significan ... | 1995 | 7619804 |
| iron regulation of siderophore biosynthesis and transport in pseudomonas putida wcs358: involvement of a transcriptional activator and of the fur protein. | pseudobactin 358 is the yellow-green fluorescent siderophore produced by pseudomonas putida wcs358 in conditions of iron limitation. the genes encoding for siderophore biosynthesis are iron-regulated at the transcriptional level. previous work has shown that a positive regulator, pfra, is absolutely required for the activation under iron-limiting conditions of pseudobactin 358 biosynthesis. in this study we identified a set of tn5 insertion mutants of strain wcs358 which lost the ability to acti ... | 1995 | 7623664 |
| degradation of polycyclic aromatic hydrocarbons (pahs) by a mixed culture and its component pure cultures, obtained from pah-contaminated soil. | a mixed culture, isolated from soil contaminated with polycyclic aromatic hydrocarbons (pahs), grew on and degraded fluoranthene in aqueous media supplemented with glucose, yeast extract, and peptone. increased complex nitrogen levels in the medium promoted bacterial growth and a greater extent of fluoranthene degradation. amendment of the media with high glucose levels also diminished specific fluoranthene degradation. the mixed culture was capable of degrading a range of other pahs, including ... | 1995 | 7627907 |
| identification of a lysine residue in the nadh-binding site of salicylate hydroxylase from pseudomonas putida s-1. | salicylate hydroxylase from pseudomonas putida s-1 was irreversibly inactivated by trinitrobenzenesulfonic acid (tnbs). the reaction was linearly dependent on tnbs concentration and the second-order rate constant was 120 m-1.min-1 for the holoprotein at ph 8.5. modification of one mole of lysine residue per mole of enzyme caused a large loss of the activity, and the enzyme was no longer able to show nadh-dehydrogenase activity after uncoupling. the presence of nadh, nad+, atp, or amp afforded pr ... | 1995 | 7629025 |
| overexpression of pseudomonas putida catechol 2,3-dioxygenase with high specific activity by genetically engineered escherichia coli. | the cloned xyle gene encoding catechol 2,3-dioxygenase (metapyrocatechase) from tol plasmid in pseudomonas putida mt-2 has been expressed in escherichia coli w3110 to a level of approximately 15% of the total soluble protein. of the total iron in the crude extract, 45% was on the enzyme. the crystallized enzyme from e. coli had higher iron content (3.7 mol/mol enzyme) and specific activity (536 u/mg) than the enzyme from p. putida mt-2. however, no differences were observed in physicochemical, p ... | 1995 | 7629031 |
| 2-oxo-1,2-dihydroquinoline 8-monooxygenase, a two-component enzyme system from pseudomonas putida 86. | 2-oxo-1,2-dihydroquinoline 8-monooxygenase, which catalyzes the nadh-dependent oxygenation of 2-oxo-1,2-dihydroquinoline to 8-hydroxy-2-oxo-1,2-dihydroquinoline, is the second enzyme in the quinoline degradation pathway of pseudomonas putida 86. this enzyme system consists of two inducible protein components, which were purified, characterized, and identified as reductase and oxygenase. the yellow reductase is a monomeric iron-sulfur flavoprotein (m(r), 38,000), containing flavin adenine dinucle ... | 1995 | 7629085 |
| integration host factor suppresses promiscuous activation of the sigma 54-dependent promoter pu of pseudomonas putida. | in the presence of m-xylene, the pu promoter of the tol plasmid of pseudomonas putida is activated by the prokaryotic enhancer-binding protein xylr. the intervening dna segment between the upstream activating sequences (uass) and those for rna polymerase binding contains an integration host factor (ihf) attachment site that is required for full transcriptional activity. in the absence of ihf, the pu promoter can be cross-activated by other members of the sigma 54-dependent family of regulatory p ... | 1995 | 7638181 |
| assessment of rapid bioassays for detecting cyanobacterial toxicity. | simple and easy-to-use bioassays with artemia salina (brine shrimp) larvae, luminescent bacteria and pseudomonas putida were evaluated for the detection of toxicity due to cyanobacterial hepato- and neurotoxins. the hepatotoxins and a neurotoxin, anatoxin-a, were extracted from laboratory-grown cultures and natural bloom samples by the solid phase fractionation method and dissolved in diluent for different bioassays. the toxin concentration of cyanobacterial extracts was determined with hplc. th ... | 1995 | 7639991 |
| catabolite-mediated mutations in alternate toluene degradative pathways in pseudomonas putida. | pseudomonas putida 54g grew on mineral salts with toluene and exhibited catechol-2,3-dioxygenase (c23o) activity, indicating a meta pathway. after 10 to 15 days on toluene, nondegrading (tol-) variants approached nearly 10% of total cfu. auxotrophs were not detected among variants, suggesting selective loss of catabolic function(s). variant formation was substrate dependent, since tol- cells were observed on neither ethylbenzene, glucose, nor peptone-based media nor when toluene catabolism was s ... | 1995 | 7642499 |
| cloning, dna sequencing, and amino acid sequencing of catechol 1,2-dioxygenases (pyrocatechase) from pseudomonas putida mt-2 and pseudomonas arvilla c-1. | catechol 1,2-dioxygenase catalyzes the oxygenative ring cleavage of catechol to form cis,cis-muconic acid and is encoded by a cata gene. we have cloned a cata gene from pseudomonas putida mt-2 using a pcr product of amino acid sequence-based primers as a probe. the amino acid sequence deduced from the 930 nucleotides was in complete agreement with the chemically determined sequence of the protein. crude extracts of escherichia coli cells carrying the cata gene downstream from the lac promoter sh ... | 1995 | 7646060 |
| energy-coupled transport and signal transduction through the gram-negative outer membrane via tonb-exbb-exbd-dependent receptor proteins. | iron in the form of ferric siderophore complexes and vitamin b12 are transported through the outer membrane of gram-negative bacteria by a mechanism which consumes energy. there is no known energy source in the outer membrane or in the adjacent periplasmic space so that energy is provided by the electrochemical potential across the cytoplasmic membrane. energy flows from the cytoplasmic into the outer membrane via a complex consisting of the tonb, exbb and exbd proteins which are anchored in the ... | 1995 | 7654405 |
| isolation of ice-nucleating active bacteria from the freeze-tolerant frog, rana sylvatica. | ice-nucleating active (ina) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (rana sylvatica) collected in winter. thirteen strains of pseudomonas fluorescens, four strains of pseudomonas putida, and two strains of enterobacter agglomerans had ice-nucleating activity. each of the ina pseudomonad strains was psychrophilic. p. putida strains were differentiated from p. fluorescens strains by gelatinase, lecithinase, and lipase production. the maximum nucleation tem ... | 1995 | 7656570 |
| purification and crystallization of benzoylformate decarboxylase. | a new large-scale purification method for benzoylformate decarboxylase from pseudomonas putida has allowed us to undertake an x-ray crystallographic study of the enzyme. the previously observed instability of the enzyme was overcome by addition of 100 microm thiamine pyrophosphate to buffers used in the purification. the final enzyme preparation was more than 97% pure, as determined by denaturing gel electrophoresis and densitometry. the mobility of the enzyme on a gel filtration column indicate ... | 1995 | 7663351 |
| growth phase-dependent induction of stationary-phase promoters of escherichia coli in different gram-negative bacteria. | rsf1010-derived plasmids carrying a fusion of a promoterless lacz gene with the sigma s-dependent growth phase-regulated promoters of escherichia coli, bolap1 and fic, were constructed. the plasmids were mobilized into the gram-negative bacterial species acetobacter methanolicus, xanthomonas campestris, pseudomonas putida, and rhizobium meliloti. the beta-galactosidase activities of bacterial cultures were determined during exponential and stationary growth phases. transcriptional activation of ... | 1995 | 7665531 |