| the isolation and functional identification on producing cellulase of pseudomonas mendocina. | the straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of n,p,k and other effective elements in ecological system. we isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in northeast of china, which can produce cellulase efficiently. the strain was identified as pseudomonas mendocina by using morphological, physiological, biochemical test, and molecular b ... | 2016 | 27710430 |
| finished genome sequence and methylome of the cyanide-degrading pseudomonas pseudoalcaligenes strain cect5344 as resolved by single-molecule real-time sequencing. | pseudomonas pseudoalcaligenes cect5344 tolerates cyanide and is also able to utilize cyanide and cyano-derivatives as a nitrogen source under alkaline conditions. the strain is considered as candidate for bioremediation of habitats contaminated with cyanide-containing liquid wastes. information on the genome sequence of the strain cect5344 became available previously. the p. pseudoalcaligenes cect5344 genome was now resequenced by applying the single molecule, real-time (smrt(®)) sequencing tech ... | 2016 | 27060556 |
| efficient production of polyhydroxyalkanoates (phas) from pseudomonas mendocina psu using a biodiesel liquid waste (blw) as the sole carbon source. | conditions for the optimal production of polyhydroxyalkanoate (pha) by pseudomonas mendocina psu using a biodiesel liquid waste (blw) were determined by response surface methodology. these were an initial carbon to nitrogen ratio (c/n) of 40 (mole/mole), an initial ph of 7.0, and a temperature of 35 °c. a biomass and pha concentration of 3.65 g/l and about 2.6 g/l (77% dcw), respectively, were achieved in a growth associated process using 20 g/l glycerol in the blw after 36 h of exponential grow ... | 2016 | 26981955 |
| enhancement of medium-chain-length polyhydroxyalkanoates biosynthesis from glucose by metabolic engineering in pseudomonas mendocina. | to enhance the biosynthesis of medium-chain-length polyhydroxyalkanoates (phamcl) from glucose in pseudomonas mendocina nk-01, metabolic engineering strategies were used to block or enhance related pathways. | 2016 | 26476529 |
| isolation and characterization of bacteria that use furans as the sole carbon source. | five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. based on 16s rrna phylogenetic analysis, these strains were identified as cupriavidus pinatubonensis (designated als1280), pigmentiphaga sp. (als1172), pseudomonas sp. bwdy (als1279), pseudomonas mendocina (als1131), and pseudomonas putida (als1267). in all cases, growth under oxygenated conditions on furfural was accompanied by the transient accumulation of 2-furo ... | 2016 | 26419660 |
| an upp-based markerless gene replacement method for genome reduction and metabolic pathway engineering in pseudomonas mendocina nk-01 and pseudomonas putida kt2440. | a markerless gene replacement method was adapted by combining a suicide plasmid, pex18tc, with a counterselectable marker, the upp gene encoding uracil phosphoribosyltransferase (uprtase), for the medium-chain length polyhydroxyalkanoates (pha(mcl))-producing strain pseudomonas mendocina nk-01. an nk-01 5-fluorouracil (5-fu) resistant background strain was first constructed by deleting the chromosomal upp gene. the suicide plasmid pex18tc, carrying a functional allele of the upp gene of p. mendo ... | 2015 | 25828098 |
| emulating structural stability of pseudomonas mendocina lipase: in silico mutagenesis and molecular dynamics studies. | the need of alkaline detergent-stable lipases has been growing rapidly as they are highly attractive for the production of detergents, biodiesel, pharmaceuticals agents, and various other applications. lipase from pseudomonas mendocina (pml) is one such candidate with triglyceride activity and non-homologous with other reported pseudomonas lipases. the present work provides insights on the role of amino acids toward structural stability of pml. pml was subjected to mutagenesis through in silico ... | 2014 | 25367042 |
| nitric oxide removal by wastewater bacteria in a biotrickling filter. | nitric oxide (no) is one of the most important air pollutants in atmosphere mainly emitted from combustion source. a biotrickling filter was designed and operated to remove no from an air stream using bacteria extracted from the sewage sludge of a municipal sewage treatment plant. to obtain the best operation conditions for the biotrickling filter, orthogonal experiments (l9(3(4))) were designed. inlet oxygen concentration was found to be the most significant factor of the biotrickling filter an ... | 2014 | 25079268 |
| enhanced anaerobic biodegradation of ocdd-contaminated soils by pseudomonas mendocina nsysu: microcosm, pilot-scale, and gene studies. | in this study, microcosm and pilot-scale experiments were performed to investigate the capability and effectiveness of pseudomonas mendocina nsysu (p. mendocina nsysu) on the bioremediation of octachlorodibenzo-p-dioxin (ocdd)-contaminated soils. the objectives were to evaluate the (1) characteristics of p. mendocina nsysu, (2) feasibility of enhancing ocdd biodegradation with the addition of p. mendocina nsysu and lecithin, and (3) variation in microbial diversity and genes responsible for the ... | 2014 | 24997259 |
| aerobic microbial fe acquisition from ferrihydrite nanoparticles: effects of crystalline order, siderophores, and alginate. | this research compared the bioavailability of fe associated with two forms of the hydrous fe oxyhydroxide nanomineral ferrihydrite (fh)--the smaller (1-3 nm), less ordered 2-line (2l) phase and the slightly larger, (2-6 nm) more ordered 6-line (6l) phase--to the common aerobic soil bacterium pseudomonas mendocina ymp. p. mendocina can acquire fe from minerals using high-affinity fe(iii) binding ligands known as siderophores and a cell-associated metalloreductase that requires direct cell-mineral ... | 2014 | 24978298 |
| optimization of medium composition for 3-hydroxycarboxylic acid production by pseudomonas mendocina-biodegraded polyhydroxybutyrate. | we optimized the culture medium for 3-hydroxycarboxylic acid production by pseudomonas mendocina ds-04-t-biodegraded polyhydroxybutyrate (phb) using the plackett-burman design, steepest ascent method, and box-behnken design. the optimized concentrations of the constituents of the culture medium were as follows: phb (7.57 g/l), nh4 cl (5.0 g/l), kh2 po4 (2.64 g/l), na2 hpo4 ·12h2 o (12 g/l), mgso4 ·7h2 o (0.5 g/l), and cacl2 ·2h2 o (5 mg/l). the yield of 3-hydroxycarboxylic acid obtained using th ... | 2015 | 24919602 |
| calcium-mediated modulation of pseudomonas mendocina nr802 biofilm influences the phenanthrene degradation. | a potential biofilm forming and phenanthrene utilizing marine bacterium pseudomonas mendocina nr802 was isolated from rushukulya, odisha, east coast of india. the effect of ca(2+) and mg(2+) on biofilm growth and phenanthrene degradation was evaluated. among the various tested concentrations, 20 mm of ca(2+) and mg(2+) showed a significant enhancement in biofilm production by the bacterium. the sem-edax study showed that the elemental composition of the biofilm varied significantly when grown in ... | 2014 | 24216621 |
| purification and characterization of two extracellular polyhydroxyalkanoate depolymerases from pseudomonas mendocina. | two polyhydroxyalkanoate depolymerases, phaase i and phaase ii, were purified to homogeneity from the culture supernatant of an effective pha-degrading bacterium, pseudomonas mendocina ds04-t. the molecular masses of phaase i and phaase ii were determined by sds-page as 59.4 and 33.8 kda, respectively. their optimum ph values were 8.5 and 8, respectively. enzymatic activity was optimal at 50 °c. both purified enzymes could degrade phb, phbv, and p(3hb-co-4hb). addition of na(+) and k(+) slightly ... | 2013 | 23881326 |
| [screening, denitrification characteristics, and anaerobic ammonium oxidation ability of denitrifying bacterium ahd7]. | a highly efficient denitrifying bacterium ahd7 was screened from activated sludge. after static culture at 30 degrees c for 3 days, the denitrification rate of the ahd7 reached 91.7%, and during denitrification, nitrite had lower accumulation, with its concentration basically maintained at 1.8 mg x l(-1). the microscopy observation demonstrated that the ahd7 was a gram-negative bacillus, with an average size of 0.5 microm x (1.5-2.5) microm. based on its biochemical/morphological characteristics ... | 2012 | 23431796 |
| comparison of medium-chain-length polyhydroxyalkanoates synthases from pseudomonas mendocina nk-01 with the same substrate specificity. | the medium-chain-length polyhydroxyalkanoate (phamcl) synthase genes phac1 and phac2 of pseudomonas mendocina nk-01 were cloned and inserted into expression plasmid pbbr1mcs-2 to form pbbr1mcs-c1 and pbbr1mcs-c2 which were expressed respectively in the phamcl-negative strain p. mendocina c7 whose phamcl synthesis operon was defined knock out. p. mendocina c7 derivatives p. mendocina c7c1 and c7c2 carrying pbbr1mcs-c1 and pbbr1mcs-c2 respectively were constructed. fermentation and gel permeation ... | 2013 | 23238264 |
| a case of an atypical femoral fracture associated with bacterial biofilm--pathogen or bystander? | we report a case of an 86-year-old woman with an atypical femoral fracture (aff) who was treated with intramedullary nailing followed by lateral femoral plating. she developed a second femoral shaft fracture distal to the intramedullary nail which required a second operation. biopsy of the periosteum overlying the site of the initial proximal aff was sent for pathogen analysis. using the ibis t5000 platform and the bac plate assay, a polymicrobial infection was diagnosed consisting of bifidobact ... | 2013 | 23229469 |
| characterization of pseudomonas mendocina lr capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with cyperus alternifolius l. | a new strain of denitrifying bacterium, pseudomonas mendocina lr, was isolated from rhizosphere soil of aquatic plants living in a river contaminated with industrial wastewater and domestic sewage. the isolate was found to fully remove as much as 613.2 mg nitrate in 60 h under stationary culture conditions. the effects of carbon sources and nitrogen sources on nitrogen removal were investigated using a modified denitrification medium (dm). sodium citrate was identified as the most effective sour ... | 2012 | 22633966 |
| augmented production of alginate oligosaccharides by the pseudomonas mendocina nk-01 mutant. | pseudomonas mendocina nk-01 can simultaneously synthesize medium-chain-length polyhydroxyalkanoate (pha(mcl)) and alginate oligosaccharides (ao) from glucose under conditions of limited nitrogen. in this study, the pha(mcl) synthesis pathway was blocked by a deletion of approximately 57% of the sequence of pha synthase operon mediated by the suicide plasmid, pex18tcc1zc2amp. deletion of the pha synthase operon in p. mendocina nk-01 was confirmed by polymerase chain reaction (pcr) and antibiotic ... | 2012 | 22429775 |
| characteristics of an aerobic denitrifier that utilizes ammonium and nitrate simultaneously under the oligotrophic niche. | an aerobic denitrifier was isolated from the hua-jia-chi pond in china and identified as pseudomonas mendocina 3-7 (genbank no. hq285879). this isolated strain could express periplasmic nitrate reductase which is essential for aerobic denitrification occurred when the dissolved oxygen (do) level maintains at 3-10 mg l(-1). | 2012 | 22392687 |
| diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in liangshui river of beijing, china. | aerobic denitrification is a process reducing the nitrate into gaseous nitrogen forms in the presence of oxygen gas, which makes the nitrification and denitrification performed simultaneously. however, little was known on the diversity of the culturable aerobic denitrifying bacteria in the surface water system. in this study, 116 strains of aerobic denitrifying bacteria were isolated from the sediment, water and biofilm samples in liangshui river of beijing. these bacteria were classified into 1 ... | 2017 | 28855587 |
| biodegradation of p(3hb-co-4hb) powder by pseudomonas mendocina for preparation low-molecular-mass p(3hb-co-4hb). | poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (p(3hb-co-4hb)) is a biodegradable plastic that is extensively utilized in many fields. in this work, p(3hb-co-4hb) powder was degraded by pseudomonas mendocina for the preparation of low-molecular-mass (lmw) p(3hb-co-4hb). after degradation, the remaining p(3hb-co-4hb) powder was analyzed via gel permeation chromatography (gpc), differential scanning calorimetry (dsc), x-ray powder diffraction (xrd), fourier transform infrared (ftir) spectroscopy, an ... | 2017 | 28828288 |
| degradation and conversion of endosulfan by newly isolated pseudomonas mendocina zam1 strain. | endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. a novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as zam1 strain. the zam1 bacterial strain was further identified as pseudomonas men ... | 2017 | 28667651 |
| pseudomonas mendocina: the first case of peritonitis on peritoneal dialysis. | | 2017 | 28655401 |
| phenotypic and genomic survey on organic acid utilization profile of pseudomonas mendocina strain s5.2, a vineyard soil isolate. | root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. the exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. in this study, a comprehensive survey on the utilization ... | 2017 | 28655216 |