| microbial metabolites and bacterial diversity in beef stored in different packaging conditions monitored by pyrosequencing, pcr-dgge, spme-gc/ms and 1hnmr. | beef chops were stored at 4°c in different conditions: air (a); modified atmosphere packaging (map); vacuum (v); bacteriocin-activated antimicrobial packaging (av). after 0 to 45 days of storage, analyses were performed to determine loads of spoilage microorganisms, microbial metabolites by spme-gc/ms and (1)hnmr, microbial diversity by pcr-dgge and pyrosequencing. the microbiological shelf-life of meat increased with increasing selectivity of storage conditions. the culture-independent analysis ... | 2011 | 21803905 |
| effect of chitosan in meat preservation. | the effect of chitosan in meat preservation, including microbiological, chemical, sensory and color qualities, was examined. in liquid medium, chitosan (0·01%) inhibited the growth of some spoilage bacteria such as bacillus subtilis ifo 3025, escherichia coli rb, pseudomonas fragi ifo 3458 and staphylococcus aureus iam 1011. at higher concentrations (0·1 and 1·0%) it inhibited the growth of the meat starter cultures, lactobacillus plantarium iam 1216, pediococcus pentosaceus iam 12296 and microc ... | 1994 | 22059662 |
| widespread head-to-head hydrocarbon biosynthesis in bacteria and role of olea. | previous studies identified the oleabcd genes involved in head-to-head olefinic hydrocarbon biosynthesis. the present study more fully defined the oleabcd protein families within the thiolase, alpha/beta-hydrolase, amp-dependent ligase/synthase, and short-chain dehydrogenase superfamilies, respectively. only 0.1 to 1% of each superfamily represents likely ole proteins. sequence analysis based on structural alignments and gene context was used to identify highly likely ole genes. selected microor ... | 2010 | 20418421 |
| the multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the arabidopsis thaliana protein mfp2. | plant fatty acids can be completely degraded within the peroxisomes. fatty acid degradation plays a role in several plant processes including plant hormone synthesis and seed germination. two multifunctional peroxisomal isozymes, mfp2 and aim1, both with 2-trans-enoyl-coa hydratase and l-3-hydroxyacyl-coa dehydrogenase activities, function in mouse ear cress (arabidopsis thaliana) peroxisomal beta-oxidation, where fatty acids are degraded by the sequential removal of two carbon units. a deficien ... | 2010 | 20463021 |
| crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites. | the crystal structure of the full-length rat peroxisomal multifunctional enzyme, type 1 (rpmfe1), has been determined at 2.8 a resolution. this enzyme has three catalytic activities and two active sites. the n-terminal part has the crotonase fold, which builds the active site for the delta(3),delta(2)-enoyl-coa isomerase and the delta(2)-enoyl-coa hydratase-1 catalytic activities, and the c-terminal part has the (3s)-hydroxyacyl-coa dehydrogenase fold and makes the (3s)-hydroxyacyl-coa dehydroge ... | 2010 | 20463028 |
| infection of orthopedic implants with emphasis on bacterial adhesion process and techniques used in studying bacterial-material interactions. | staphylococcus comprises up to two-thirds of all pathogens in orthopedic implant infections and they are the principal causative agents of two major types of infection affecting bone: septic arthritis and osteomyelitis, which involve the inflammatory destruction of joint and bone. bacterial adhesion is the first and most important step in implant infection. it is a complex process influenced by environmental factors, bacterial properties, material surface properties and by the presence of serum ... | 2012 | 23507884 |
| a novel cold-active lipase from candida albicans: cloning, expression and characterization of the recombinant enzyme. | a novel lipase gene lip5 from the yeast candida albicans was cloned and sequenced. alignment of amino acid sequences revealed that 86-34% identity exists with lipases from other candida species. the lipase and its mutants were expressed in the yeast pichia pastoris, where alternative codon usage caused the mistranslation of 154-ser and 293-ser as leucine. 154-ser to leucine resulted in loss of expression of lip5, and 293-ser to leucine caused a marked reduction in the lipase activity. lip5-dm, w ... | 2011 | 21747717 |
| comparison of two optical-density-based methods and a plate count method for estimation of growth parameters of bacillus cereus. | quantitative microbiological models predicting proliferation of microorganisms relevant for food safety and/or food stability are useful tools to limit the need for generation of biological data through challenge testing and shelf-life testing. the use of these models requires quick and reliable methods for the generation of growth data and estimation of growth parameters. growth parameter estimation can be achieved using methods based on plate counting and methods based on measuring the optical ... | 2010 | 20081006 |
| microbiological and sensory effects of the combined application of hot-cold organic acid sprays and steam condensation at subatmospheric pressure for decontamination of inoculated pig tissue surfaces. | we studied microbiological and sensory effects of treating pig tissue for 15 s with 55 and 10-¦c sprays of acetic acid (aa; 0.15 to 0.3 m) and lactic acid (la; 0.1 to 0.2 m) solutions prior to the tissue being subjected to steam condensation (18 s at 65-¦c or 10 s at 75-¦c). la or aa spraying and then steam treatment resulted in 3- to 4-log average reductions of pseudomonas fragi and yersinia enterocolitica inocula (6 to 7 log cfu/cm(2)), regardless of acid temperature or concentration. buffered ... | 2011 | 21819663 |
| search and discovery strategies for biotechnology: the paradigm shift. | profound changes are occurring in the strategies that biotechnology-based industries are deploying in the search for exploitable biology and to discover new products and develop new or improved processes. the advances that have been made in the past decade in areas such as combinatorial chemistry, combinatorial biosynthesis, metabolic pathway engineering, gene shuffling, and directed evolution of proteins have caused some companies to consider withdrawing from natural product screening. in this ... | 2000 | 10974127 |
| in silico sequence analysis and molecular modeling of the three-dimensional structure of dahp synthase from pseudomonas fragi. | the shikimate pathway is involved in production of aromatic amino acids in microorganisms and plants. the enzymes of this biosynthetic pathway are a potential target for the design of antimicrobial compounds and herbicides. 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase (dahps) catalyzes the first step of the pathway. the gene encoding dahps was cloned and sequenced from pseudomonas fragi, the bacterium responsible for spoilage of milk, dairy products and meat. amino acid sequence deduced f ... | 2011 | 20517625 |
| different molecular types of pseudomonas fragi have the same overall behaviour as meat spoilers. | the functional diversity of a population of sixty-five different strains of p. fragi isolated from fresh and spoiled meat was studied in order to evaluate the population heterogeneity related to meat spoilage potential. the strains were characterized for the proteolytic activity at 4 degrees c on beef sarcoplasmic proteins and only 9 strains were found to be proteolytic. an iron-dependent growth behaviour was shown when each strain was grown in citrate medium containing either myoglobin, haemogl ... | 2010 | 20627208 |
| quinolones: from antibiotics to autoinducers. | since quinine was first isolated, animals, plants and microorganisms producing a wide variety of quinolone compounds have been discovered, several of which possess medicinally interesting properties ranging from antiallergenic and anticancer to antimicrobial activities. over the years, these have served in the development of many synthetic drugs, including the successful fluoroquinolone antibiotics. pseudomonas aeruginosa and related bacteria produce a number of 2-alkyl-4(1h)-quinolones, some of ... | 2011 | 20738404 |
| biofilms: microbial life on surfaces. | microorganisms attach to surfaces and develop biofilms. biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (eps) matrix, reduced growth rates, and the up- and down- regulation of specific genes. attachment is a complex process regulated by diverse characteristics of the growth medium, substratum, and cell surface. an established biofilm structure comprises microbial cells and eps, has a defined architecture, and ... | 2002 | 12194761 |
| dolichol phosphate mannose synthase1 mediates the biogenesis of isoprenyl-linked glycans and influences development, stress response, and ammonium hypersensitivity in arabidopsis. | the most abundant posttranslational modification in nature is the attachment of preassembled high-mannose-type glycans, which determines the fate and localization of the modified protein and modulates the biological functions of glycosylphosphatidylinositol-anchored and n-glycosylated proteins. in eukaryotes, all mannose residues attached to glycoproteins from the luminal side of the endoplasmic reticulum (er) derive from the polyprenyl monosaccharide carrier, dolichol p-mannose (dol-p-man), whi ... | 2011 | 21558543 |
| quinolones: from antibiotics to autoinducers. | since quinine was first isolated, animals, plants and microorganisms producing a wide variety of quinolone compounds have been discovered, several of which possess medicinally interesting properties ranging from antiallergenic and anticancer to antimicrobial activities. over the years, these have served in the development of many synthetic drugs, including the successful fluoroquinolone antibiotics. pseudomonas aeruginosa and related bacteria produce a number of 2-alkyl-4(1h)-quinolones, some of ... | 2011 | 20738404 |
| bioinformatic analysis of the genome of infectious salmon anemia virus associated with outbreaks with high mortality in chile. | the infectious salmon anemia virus (isav), an orthomyxovirus, is the major cause of outbreaks of high mortality rates in salmon in chile. it has been proposed that the virulence of isav isolates lies mainly in hemagglutinin-esterase and fusion glycoproteins. however, based on current information, the contribution of other viral genes cannot be ruled out. to study this, we isolated and determined the complete coding sequence of two high-prevalence chilean isolates associated with outbreaks of hig ... | 2010 | 20810724 |
| a combined top-down and bottom-up ms approach for the characterization of hemoglobin variants in rhesus monkeys. | sickle cell disease is caused by one of the 1200 known hemoglobin variations. a single-point mutation β6(a3)glu→val leads to sickling of red blood cells, which in turn causes a lack of oxygen supply to tissue and organs. although sickle cell disease is well understood, treatment options are currently underdeveloped. the only food and drug administration-approved drug is hydroxyurea, an inducer of fetal γ-hemoglobin, which is known to have a higher oxygen affinity than adult hemoglobins and thus ... | 2010 | 20848672 |
| adaptation to cold and proteomic responses of the psychrotrophic biopreservative lactococcus piscium strain cncm i-4031. | there is considerable interest in the use of psychrotrophic bacteria for food biopreservation and in the understanding of cold adaptation mechanisms. the psychrotrophic biopreservative lactococcus piscium strain cncm i-4031 was studied for its growth behavior and proteomic responses after cold shock and during cold acclimation. growth kinetics highlighted the absence of growth latency after cold shock, suggesting a very high promptness in cold adaptation, a behavior that has never been described ... | 2010 | 20935127 |
| multiple fadd acyl-coa synthetases contribute to differential fatty acid degradation and virulence in pseudomonas aeruginosa. | a close interconnection between nutrient metabolism and virulence factor expression contributes to the pathophysiology of pseudomonas aeruginosa as a successful pathogen. p. aeruginosa fatty acid (fa) degradation is complicated with multiple acyl-coa synthetase homologs (fadds) expressed in vivo in lung tissue during cystic fibrosis infections. the promoters of two genetically linked p. aeruginosa fadd genes (fadd1 and fadd2) were mapped and northern blot analysis indicated they could exist on t ... | 2010 | 21042406 |
| pseudomonas deceptionensis sp. nov., a new psychrotolerant bacterium from the antarctic. | during the taxonomic investigation of cold-adapted bacteria from samples collected in the antarctic area of the south shetland islands one gram-negative, cold-adapted aerobic bacterium, designated strain m1(t) was isolated from marine sediment collected on deception island. the organism was rod-shaped, catalase- and oxidase positive and motile by means of a single polar flagellum. this psychrotolerant strain grew at -4°c to 34°c. the 16s rrna gene phylogenetic studies confirmed that the antarcti ... | 2010 | 21062736 |
| aerobic degradation of mercaptosuccinate by the gram-negative bacterium variovorax paradoxus strain b4. | the gram-negative bacterium variovorax paradoxus strain b4 was isolated from soil under mesophilic and aerobic conditions to elucidate the so far unknown catabolism of mercaptosuccinate (ms). during growth with ms this strain released significant amounts of sulfate into the medium. tn5::mob-induced mutagenesis was successfully employed and yielded nine independent mutants incapable of using ms as a carbon source. in six of these mutants, tn5::mob insertions were mapped in a putative gene encodin ... | 2010 | 21075928 |
| aerobic degradation of mercaptosuccinate by the gram-negative bacterium variovorax paradoxus strain b4. | the gram-negative bacterium variovorax paradoxus strain b4 was isolated from soil under mesophilic and aerobic conditions to elucidate the so far unknown catabolism of mercaptosuccinate (ms). during growth with ms this strain released significant amounts of sulfate into the medium. tn5::mob-induced mutagenesis was successfully employed and yielded nine independent mutants incapable of using ms as a carbon source. in six of these mutants, tn5::mob insertions were mapped in a putative gene encodin ... | 2010 | 21075928 |
| application of a depositional facies model to an acid mine drainage site. | lower red eyes is an acid mine drainage site in pennsylvania where low-ph fe(ii) oxidation has created a large, terraced iron mound downstream of an anoxic, acidic, metal-rich spring. aqueous chemistry, mineral precipitates, microbial communities, and laboratory-based fe(ii) oxidation rates for this site were analyzed in the context of a depositional facies model. depositional facies were defined as pools, terraces, or microterracettes based on cm-scale sediment morphology, irrespective of the d ... | 2010 | 21097582 |
| application of a depositional facies model to an acid mine drainage site. | lower red eyes is an acid mine drainage site in pennsylvania where low-ph fe(ii) oxidation has created a large, terraced iron mound downstream of an anoxic, acidic, metal-rich spring. aqueous chemistry, mineral precipitates, microbial communities, and laboratory-based fe(ii) oxidation rates for this site were analyzed in the context of a depositional facies model. depositional facies were defined as pools, terraces, or microterracettes based on cm-scale sediment morphology, irrespective of the d ... | 2010 | 21097582 |
| n-glycans modulate the function of human corticosteroid-binding globulin. | human corticosteroid-binding globulin (cbg), a heavily glycosylated protein containing six n-linked glycosylation sites, transports cortisol and other corticosteroids in blood circulation. here, we investigate the biological importance of the n-glycans of cbg derived from human serum by performing a structural and functional characterisation of cbg n-glycosylation. lc-ms/ms based glycoproteomics and glycomics combined with exoglycosidase treatment revealed 26 complex type n-glycoforms, all of wh ... | 2011 | 21558494 |
| Characterisation of histamine-producing bacteria from farmed blackspot seabream (Pagellus bogaraveo) and turbot (Psetta maxima). | Turbot (Psetta maxima) and blackspot seabream (Pagellus bogaraveo) represent two of the most important emerging farmed fish species in European countries. However, no information of the presence and development of histamine-producing bacteria on them has been reported so far. Accordingly, the aim of this study was to isolate and identify the main histamine-producing bacteria in farmed turbot and blackspot seabream. For this study, 24 isolates (12 from turbot and 12 from blackspot seabream) were ... | 2011 | 21925757 |
| Biomimetic CO2 sequestration using purified carbonic anhydrase from indigenous bacterial strains immobilized on biopolymeric materials. | The present study deals with immobilization of purified CA and whole cell of Pseudomonas fragi, Micrococcus lylae, and Micrococcus luteus 2 on different biopolymer matrices. Highest enzyme immobilization was achieved with P. fragi CA (89%) on chitosan-KOH beads, while maximum cell immobilization was achieved with M. lylae (75%) on chitosan-NH(4)OH beads. A maximum increase of 1.08-1.18 fold stability between 35 and 55°C was observed for M. lylae immobilized CA. The storage stability was improved ... | 2011 | 22112959 |
| insights into folate/fad-dependent trna methyltransferase mechanism: role of two highly conserved cysteines in catalysis. | the flavoprotein trmfo methylates specifically the c5 carbon of the highly conserved uridine 54 in trnas. contrary to most methyltransferases, the 1-carbon unit transferred by trmfo derives from 5,10-methylenetetrahydrofolate and not from s-adenosyl-l-methionine. the enzyme also employs the fad hydroquinone as a reducing agent of the c5 methylene u54-trna intermediate in vitro. by analogy with the catalytic mechanism of thymidylate synthase thya, a conserved cysteine located near the fad isoallo ... | 2011 | 21846722 |
| monitoring the succession of the biota grown on a selective medium for pseudomonads during storage of minced beef with molecular-based methods. | in the present study, the succession of the biota grown on a selective medium for pseudomonads (pseudomonas agar based medium - pab) during the storage of meat under different conditions was monitored. thus, minced beef was stored aerobically and under modified atmosphere packaging in the presence (map+) and absence (map-) of oregano essential oil at 0, 5, 10 and 15 °c. a total of 267 pure cultures were recovered from pab throughout the storage period and subjected to pcr-denaturing gradient gel ... | 2012 | 23498179 |
| sequence co-evolution gives 3d contacts and structures of protein complexes. | protein-protein interactions are fundamental to many biological processes. experimental screens have identified tens of thousands of interactions, and structural biology has provided detailed functional insight for select 3d protein complexes. an alternative rich source of information about protein interactions is the evolutionary sequence record. building on earlier work, we show that analysis of correlated evolutionary sequence changes across proteins identifies residues that are close in spac ... | 2014 | 25255213 |
| crystal structure of proteus mirabilis lipase, a novel lipase from the proteus/psychrophilic subfamily of lipase family i.1. | bacterial lipases from family i.1 and i.2 catalyze the hydrolysis of triacylglycerol between 25-45°c and are used extensively as biocatalysts. the lipase from proteus mirabilis belongs to the proteus/psychrophilic subfamily of lipase family i.1 and is a promising catalyst for biodiesel production because it can tolerate high amounts of water in the reaction. here we present the crystal structure of the proteus mirabilis lipase, a member of the proteus/psychrophilic subfamily of i.1lipases. the s ... | 2012 | 23300806 |
| balance between coiled-coil stability and dynamics regulates activity of bvgs sensor kinase in bordetella. | the two-component system bvgas controls the expression of the virulence regulon of bordetella pertussis. bvgs is a prototype of bacterial sensor kinases with extracytoplasmic venus flytrap perception domains. following its transmembrane segment, bvgs harbors a cytoplasmic per-arnt-sim (pas) domain and then a predicted 2-helix coiled coil that precede the dimerization-histidine-phosphotransfer domain of the kinase. bvgs homologs have a similar domain organization, or they harbor only a predicted ... | 2016 | 26933056 |
| characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis. | the gastrointestinal tract of sheep contain complex microbial communities that influence numerous aspects of the sheep's health and development. the objective of this study was to analyze the composition and diversity of the microbiota in the gastrointestinal tract sections (rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum) of sheep. | 2016 | 27383798 |
| antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river. | we characterized the bacterial populations in surface water receiving effluent from an oxytetracycline (otc) production plant. additional sampling sites included the receiving river water 5 km upstream and 20 km downstream from the discharge point. high levels of otc were found in the wastewater (ww), and the antibiotic was still detectable in river water downstream (rwd), with undetectable levels in river water upstream (rwu). a total of 341 bacterial strains were isolated using nonselective me ... | 2010 | 20400569 |
| biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates. | this article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. biofilm formation is a natural process where microbial cells attach to the support (adsorbent) or form flocs/aggregates (also called granules) without use of chemicals and form thick layers of cells known as "biofilms." as a result of biofilm formation, cell densities in the reactor increase and cell concentrations as high as 74 gl(-1) can be achieved. the reactor ... | 2005 | 16122390 |
| identification and characterization of a new true lipase isolated through metagenomic approach. | abstract: background: metagenomics, the application of molecular genomics to consortia of non-cultivated microbes, has the potential to have a substantial impact on the search for novel industrial enzymes such as esterases (carboxyl ester hydrolases, ec 3.1.1.1) and lipases (triacylglycerol lipases, ec 3.1.1.3). in the current work, a novel lipase gene was identified from a fosmid metagenomic library constructed with the "prokaryotic-enriched" dna from a fat-contaminated soil collected from a wa ... | 2011 | 21762508 |
| characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis. | the gastrointestinal tract of sheep contain complex microbial communities that influence numerous aspects of the sheep's health and development. the objective of this study was to analyze the composition and diversity of the microbiota in the gastrointestinal tract sections (rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum) of sheep. | 2016 | 27383798 |
| detection of food spoilage and pathogenic bacteria based on ligation detection reaction coupled to flow-through hybridization on membranes. | traditional culturing methods are still commonly applied for bacterial identification in the food control sector, despite being time and labor intensive. microarray technologies represent an interesting alternative. however, they require higher costs and technical expertise, making them still inappropriate for microbial routine analysis. the present study describes the development of an efficient method for bacterial identification based on flow-through reverse dot-blot (ft-rdb) hybridization on ... | 2014 | 24818128 |
| antibiotic multiresistance analysis of mesophilic and psychrotrophic pseudomonas spp. isolated from goat and lamb slaughterhouse surfaces throughout the meat production process. | the aim of this study was to investigate the phenotypic and genotypic antibiotic resistance profiles of pseudomonads isolated from surfaces of a goat and lamb slaughterhouse, which were representative of areas that are possible sources of meat contamination. mesophilic (85 isolates) and psychrotrophic (37 isolates) pseudomonads identified at the species level generally were resistant to sulfamethoxazole, erythromycin, amoxicillin, ampicillin, chloramphenicol, trimethoprim, rifampin, and ceftazid ... | 2014 | 25172860 |
| beneficial effects of spices in food preservation and safety. | spices have been used since ancient times. although they have been employed mainly as flavoring and coloring agents, their role in food safety and preservation have also been studied in vitro and in vivo. spices have exhibited numerous health benefits in preventing and treating a wide variety of diseases such as cancer, aging, metabolic, neurological, cardiovascular, and inflammatory diseases. the present review aims to provide a comprehensive summary of the most relevant and recent findings on ... | 2016 | 27708620 |
| melanin-templated rapid synthesis of silver nanostructures. | as a potent antimicrobial agent, silver nanostructures have been used in nanosensors and nanomaterial-based assays for the detection of food relevant analytes such as organic molecules, aroma, chemical contaminants, gases and food borne pathogens. in addition silver based nanocomposites act as an antimicrobial for food packaging materials. in this prospective, the food grade melanin pigment extracted from sponge associated actinobacterium nocardiopsis alba msa10 and melanin mediated synthesis of ... | 2014 | 24885756 |
| microbial deterioration of vacuum-packaged chilled beef cuts and techniques for microbiota detection and characterization: a review. | gas production from microbial deterioration in vacuum-packs of chilled meat leads to pack distension, which is commonly referred as blown pack. this phenomenon is attributed to some psychrophilic and psychrotrophic clostridium species, as well as enterobacteria. the ability of these microorganisms to grow at refrigeration temperatures makes the control by the meat industry a challenge. this type of deterioration has been reported in many countries including some plants in the midwestern and sout ... | 2011 | 24031598 |
| the diversity of bacteria isolated from antarctic freshwater reservoirs possessing the ability to produce polyhydroxyalkanoates. | the diversity of polyhydroxyalkanoates-producing bacteria in freshwater reservoirs in the ecology glacier foreland, antarctica, was examined by a cultivation-dependent method. isolated strains were analyzed phylogenetically by 16s rrna gene sequencing, and classified as members of alpha-, beta-, or gammaproteobacteria classes. polymerase chain reaction was used to detect pha synthase genes. potential polyhydroxyalkanoates (phas) producers belonging mainly to pseudomonas sp., and janthinobacteriu ... | 2014 | 24939384 |
| the dual role of microbes in corrosion. | corrosion is the result of a series of chemical, physical and (micro) biological processes leading to the deterioration of materials such as steel and stone. it is a world-wide problem with great societal and economic consequences. current corrosion control strategies based on chemically produced products are under increasing pressure of stringent environmental regulations. furthermore, they are rather inefficient. therefore, there is an urgent need for environmentally friendly and sustainable c ... | 2014 | 25259571 |
| the dual role of microbes in corrosion. | corrosion is the result of a series of chemical, physical and (micro) biological processes leading to the deterioration of materials such as steel and stone. it is a world-wide problem with great societal and economic consequences. current corrosion control strategies based on chemically produced products are under increasing pressure of stringent environmental regulations. furthermore, they are rather inefficient. therefore, there is an urgent need for environmentally friendly and sustainable c ... | 2014 | 25259571 |
| expression and characterization of thermotolerant lipase with broad ph profiles isolated from an antarctic pseudomonas sp strain ams3. | a gene encoding a thermotolerant lipase with broad ph was isolated from an antarctic pseudomonas strain ams3. the recombinant lipase ams3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. the molecular weight was approximately ∼60 kda including the strep and affinity tags. interestingly, the purified antarctic ams3 lipase exhibited broad temperature profile from 10-70 °c and stable over a broad ph ran ... | 2016 | 27781152 |
| discovery, molecular mechanisms, and industrial applications of cold-active enzymes. | cold-active enzymes constitute an attractive resource for biotechnological applications. their high catalytic activity at temperatures below 25°c makes them excellent biocatalysts that eliminate the need of heating processes hampering the quality, sustainability, and cost-effectiveness of industrial production. here we provide a review of the isolation and characterization of novel cold-active enzymes from microorganisms inhabiting different environments, including a revision of the latest techn ... | 2016 | 27667987 |
| isolation, cdna cloning, and structure-based functional characterization of oryctin, a hemolymph protein from the coconut rhinoceros beetle, oryctes rhinoceros, as a novel serine protease inhibitor. | we isolated oryctin, a 66-residue peptide, from the hemolymph of the coconut rhinoceros beetle oryctes rhinoceros and cloned its cdna. oryctin is dissimilar to any other known peptides in amino acid sequence, and its function has been unknown. to reveal that function, we determined the solution structure of recombinant (13)c,(15)n-labeled oryctin by heteronuclear nmr spectroscopy. oryctin exhibits a fold similar to that of kazal-type serine protease inhibitors but has a unique additional c-termi ... | 2010 | 20630859 |
| the unique biosynthetic route from lupinus beta-conglutin gene to blad. | during seed germination, beta-conglutin undergoes a major cycle of limited proteolysis in which many of its constituent subunits are processed into a 20 kda polypeptide termed blad. blad is the main component of a glycooligomer, accumulating exclusively in the cotyledons of lupinus species, between days 4 and 12 after the onset of germination. | 2010 | 20066045 |
| high voltage electrochemiluminescence (ecl) as a new method for detection of pah during screening for pah-degrading microbial consortia. | the search for new bacterial consortia capable of removing pah from the environment is associated with the need to employ novel, simple, and economically efficient detection methods. a fluorimetric method (fl) as well as high voltage electrochemiluminescence (ecl) on a modified surface of an aluminum electrode were used in order to determine the changes in the concentrations of pah in the studied aqueous solutions. the ecl signal (the spectrum and emission intensity for a given wavelength) was d ... | 2015 | 26213425 |
| dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution. | biodiesels are methyl esters of fatty acids that are usually produced by base catalyzed transesterification of triacylglyerol with methanol. some lipase enzymes are effective catalysts for biodiesel synthesis and have many potential advantages over traditional base or acid catalyzed transesterification. natural lipases are often rapidly inactivated by the high methanol concentrations used for biodiesel synthesis, however, limiting their practical use. the lipase from proteus mirabilis is a parti ... | 2013 | 23648063 |
| functional characterization and evaluation of in vitro protective efficacy of murine monoclonal antibodies burk24 and burk37 against burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis has been recognized by cdc as a category b select agent. although substantial efforts have been made for development of vaccine molecules against the pathogen, significant hurdles still remain. with no licensed vaccines available and high relapse rate of the disease, there is a pressing need for development of alternate protection strategies. antibody-mediated passive protection is promising in this regard and our primary interest was ... | 2014 | 24614539 |
| bactquant: an enhanced broad-coverage bacterial quantitative real-time pcr assay. | bacterial load quantification is a critical component of bacterial community analysis, but a culture-independent method capable of detecting and quantifying diverse bacteria is needed. based on our analysis of a diverse collection of 16 s rrna gene sequences, we designed a broad-coverage quantitative real-time pcr (qpcr) assay--bactquant--for quantifying 16 s rrna gene copy number and estimating bacterial load. we further utilized in silico evaluation to complement laboratory-based qpcr characte ... | 2012 | 22510143 |
| recombinant lipases and phospholipases and their use as biocatalysts for industrial applications. | lipases and phospholipases are interfacial enzymes that hydrolyze hydrophobic ester linkages of triacylglycerols and phospholipids, respectively. in addition to their role as esterases, these enzymes catalyze a plethora of other reactions; indeed, lipases also catalyze esterification, transesterification and interesterification reactions, and phospholipases also show acyltransferase, transacylase and transphosphatidylation activities. thus, lipases and phospholipases represent versatile biocatal ... | 2015 | 26340621 |
| efficacy of ph elevation as a bactericidal strategy for treating ballast water of freight carriers. | treatment of ship ballast water with sodium hydroxide (naoh) is one method currently being developed to minimize the risk to introduce aquatic invasive species. the bactericidal capability of sodium hydroxide was determined for 148 bacterial strains from ballast water collected in 2009 and 2010 from the m/v indiana harbor, a bulk-freight carrier plying the laurentian great lakes, usa. primary culture of bacteria was done using brain heart infusion agar and a developmental medium. strains were ch ... | 2015 | 26257948 |
| lipase improvement: goals and strategies. | | 2012 | 24688646 |
| enhancing functional production of a chaperone-dependent lipase in escherichia coli using the dual expression cassette plasmid. | the lipase subfamilies i.1 and i.2 show more than 33% homology in the amino acid sequences and most members share another common property that their genes are clustered with the secondary genes whose protein products are required for folding the lipase into an active conformation and secretion into the culture medium. in previous studies, the lipase (lipa) and its chaperone (lipb) from ralstonia sp. m1 were overexpressed in e. coli and the lipase was successfully refolded in vitro. the purpose o ... | 2012 | 22380513 |
| post-mortem volatiles of vertebrate tissue. | volatile emission during vertebrate decay is a complex process that is understood incompletely. it depends on many factors. the main factor is the metabolism of the microbial species present inside and on the vertebrate. in this review, we combine the results from studies on volatile organic compounds (vocs) detected during this decay process and those on the biochemical formation of vocs in order to improve our understanding of the decay process. micro-organisms are the main producers of vocs, ... | 2011 | 21720824 |
| quorum sensing in the context of food microbiology. | food spoilage may be defined as a process that renders a product undesirable or unacceptable for consumption and is the outcome of the biochemical activity of a microbial community that eventually dominates according to the prevailing ecological determinants. although limited information are reported, this activity has been attributed to quorum sensing (qs). consequently, the potential role of cell-to-cell communication in food spoilage and food safety should be more extensively elucidated. such ... | 2012 | 22706047 |
| toward stable genetic engineering of human o-glycosylation in plants. | glycosylation is the most abundant and complex posttranslational modification to be considered for recombinant production of therapeutic proteins. mucin-type (n-acetylgalactosamine [galnac]-type) o-glycosylation is found in eumetazoan cells but absent in plants and yeast, making these cell types an obvious choice for de novo engineering of this o-glycosylation pathway. we previously showed that transient implementation of o-glycosylation capacity in plants requires introduction of the synthesis ... | 2012 | 22791304 |
| nutritional and hygienic quality of raw milk in the mid-northern region of algeria: correlations and risk factors. | this paper aims to study the overall quality of raw milk in the mid-northern region of algeria. the analysis results showed a decrease in the average temperature for the delivery of 1,54°c with p<0.001. however, no significant variation (p>0.05) was observed in almost all the physical and nutritional parameters studied (ph, fat content, and protein content) between m1 and m2. the average contamination by total mesophilic aerobic bacteria (tmab), coliforms, yeasts, molds, and different pathogens ... | 2014 | 25374932 |
| preliminary investigation on the use of allyi isothiocyanate to increase the shelf-life of gilthead sea bream (sparus aurata) fillets. | the aim of this work is to evaluate the activity of allyl isothiocyanate (aitc) against fish spoilage bacteria (specific spoilage organisms; ssos) as well as its possible use in gilthead sea bream (sparus aurata) fillets to extend their shelf-life. in this regard, in vitro tests are carried out in order to evaluate the inhibitory activity of aitc and its vapours on several strains of ssos. the aitc effect on the shelf-life of sea bream fillets was made by putting them in plastic trays hermetical ... | 2015 | 27800402 |
| identification of microorganisms in duck meat products available in korea and the effect of high hydrostatic pressure. | the objective of this study was to investigate the microbial count of duck meat and duck meat products commercially available in korea. high hydrostatic pressure (hhp) treatment was applied at 0.1, 300, 400, and 500 mpa for 5 min to enhance the microbiological safety of duck meats. the levels of total aerobic bacteria were in the ranges of 3.53-6.19 and 3.62-6.85 log cfu/g in raw and smoked duck products, respectively. by dna sequence analysis, we identified microorganisms responsible for spoila ... | 2016 | 27194939 |
| genome sequences of pseudomonas fragi strains a22 and b25. | pseudomonas fragi a22 is a novel isolate that produces bead-like particles (a22b) in its cell wall. to explore the genetic basis for the formation of a22b, p. fragi a22 and the type strain of the species, p. fragi b25, were subjected to genome sequence analysis. here, we report the draft genome sequences and automatic annotation of both strains. these data offer a solid base for related studies of p. fragi, including comparative genomics, proteomics, and gene mining. | 2012 | 22628506 |
| use of antimicrobial food additives as potential dipping solutions to control pseudomonas spp. contamination in the frankfurters and ham. | this study evaluated the effect of sodium diacetate and sodium lactate solutions for reducing the cell count of pseudomonas spp. in frankfurters and hams. a mixture of pseudomonas aeruginosa (nccp10338, nccp10250, and nccp11229), and pseudomonas fluorescens (kacc10323 and kacc10326) was inoculated on cooked frankfurters and ham. the inoculated samples were immersed into control (sterile distilled water), sodium diacetate (5 and 10%), sodium lactate (5 and 10%), 5% sodium diacetate + 5% sodium la ... | 2014 | 26761492 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| antimicrobial effect of malpighia punicifolia and extension of water buffalo steak shelf-life. | in the present study, a multiple approach was used to characterize malpighia punicifolia extract and to evaluate its inhibitory activity against several meat spoilage bacteria. first, volatile fraction, vitamins and phenolic compounds of the extract obtained by supercritical fluid extraction were determined by gc-ms and hplc. then, the antimicrobial action of the extract was in vitro evaluated against pseudomonas putida dsmz 291(t), pseudomonas fluorescens dsmz 50009(t), pseudomonas fragi dsmz 3 ... | 2015 | 26556435 |
| subcellular targeting of salmonella virulence proteins by host-mediated s-palmitoylation. | several pathogenic bacteria utilize type iii secretion systems (ttss) to deliver into host cells bacterial virulence proteins with the capacity to modulate a variety of cellular pathways. once delivered into host cells, the accurate targeting of bacterial effectors to specific locations is critical for their proper function. however, little is known about the mechanisms these virulence effectors use to reach their subcellular destination. here we show that the salmonella ttss effector proteins s ... | 2011 | 21767808 |
| spoilage-related activity of carnobacterium maltaromaticum strains in air-stored and vacuum-packed meat. | one hundred and three isolates of carnobacterium spp. from raw meat were analyzed by rapd-pcr and identified by 16s rrna gene sequencing. forty-five strains of c. maltaromaticum were characterized for their growth capability at different temperatures, nacl concentrations and ph values and for in vitro lipolytic and proteolytic activities. moreover, their spoilage potential in meat was investigated by analyzing the release of volatile organic compounds (vocs) in meat stored in air or vacuum pack. ... | 2011 | 21784913 |
| hydrolytic potential of a psychrotrophic pseudomonas isolated from refrigerated raw milk. | the production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a pseudomonas sp. belonging to the pseudomonas jenssenii group, was studied. this bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). high levels of α-glucosidase were produced in skim milk broth. hydrolytic enzymes detected in skim milk broth are of particular concern, indicating ... | 2011 | 24031781 |
| metabolic engineering of escherichia coli for the production of xylonate. | xylonate is a valuable chemical for versatile applications. although the chemical synthesis route and microbial conversion pathway were established decades ago, no commercial production of xylonate has been obtained so far. in this study, the industrially important microorganism escherichia coli was engineered to produce xylonate from xylose. through the coexpression of a xylose dehydrogenase (xdh) and a xylonolactonase (xylc) from caulobacter crescentus, the recombinant strain could convert 1 g ... | 2013 | 23861757 |
| antimicrobial effect of malpighia punicifolia and extension of water buffalo steak shelf-life. | in the present study, a multiple approach was used to characterize malpighia punicifolia extract and to evaluate its inhibitory activity against several meat spoilage bacteria. first, volatile fraction, vitamins and phenolic compounds of the extract obtained by supercritical fluid extraction were determined by gc-ms and hplc. then, the antimicrobial action of the extract was in vitro evaluated against pseudomonas putida dsmz 291(t), pseudomonas fluorescens dsmz 50009(t), pseudomonas fragi dsmz 3 ... | 2015 | 26556435 |
| pseudomonas fluorescens pirates both ferrioxamine and ferricoelichelin siderophores from streptomyces ambofaciens. | iron is essential in many biological processes. however, its bioavailability is reduced in aerobic environments, such as soil. to overcome this limitation, microorganisms have developed different strategies, such as iron chelation by siderophores. some bacteria have even gained the ability to detect and utilize xenosiderophores, i.e., siderophores produced by other organisms. we illustrate an example of such an interaction between two soil bacteria, pseudomonas fluorescens strain bbc6r8 and stre ... | 2015 | 25724953 |
| a vector system for abc transporter-mediated secretion and purification of recombinant proteins in pseudomonas species. | pseudomonas fluorescens is an efficient platform for recombinant protein production. p. fluorescens has an abc transporter secreting endogenous thermostable lipase (tlia) and protease, which can be exploited to transport recombinant proteins across the cell membrane. in this study, the expression vector pdart was constructed by inserting tlidef, genes encoding the abc transporter, along with the construct of the lipase abc transporter recognition domain (lard), into pdsk519, a widely used shuttl ... | 2015 | 25548043 |
| the effect of a novel low temperature-short time (ltst) process to extend the shelf-life of fluid milk. | pasteurization has long been the standard method to extend the shelf-life of dairy products, as well as a means to reduce microbial load and the risk of food-borne pathogens. however, the process has limitations, which include cost effectiveness, high energy input, and reduction of product quality/organoleptic characteristics. in an effort to reduce these limitations and extend shelf-life, this study examined a novel low temperature, short time (ltst) method in which dispersed milk in the form o ... | 2016 | 27350902 |
| 4-coumarate:coenzyme a ligase has the catalytic capacity to synthesize and reuse various (di)adenosine polyphosphates. | 4-coumarate:coenzyme a ligase (4cl) is known to activate cinnamic acid derivatives to their corresponding coenzyme a esters. as a new type of 4cl-catalyzed reaction, we observed the synthesis of various mono- and diadenosine polyphosphates. both the native 4cl2 isoform from arabidopsis (at4cl2 wild type) and the at4cl2 gain of function mutant m293p/k320l, which exhibits the capacity to use a broader range of phenolic substrates, catalyzed the synthesis of adenosine 5'-tetraphosphate (p(4)a) and ... | 2003 | 12644689 |
| outcome of a workshop on applications of protein models in biomedical research. | we describe the proceedings and conclusions from the "workshop on applications of protein models in biomedical research" (the workshop) that was held at the university of california, san francisco on 11 and 12 july, 2008. at the workshop, international scientists involved with structure modeling explored (i) how models are currently used in biomedical research, (ii) the requirements and challenges for different applications, and (iii) how the interaction between the computational and experimenta ... | 2009 | 19217386 |
| analysis of isoaspartic acid by selective proteolysis with asp-n and electron transfer dissociation mass spectrometry. | a ubiquitous yet underappreciated protein post-translational modification, isoaspartic acid (isoasp, isod, or beta-asp), generated via the deamidation of asparagine or isomerization of aspartic acid in proteins, plays a diverse and crucial role in aging, as well as autoimmune, cancer, neurodegeneration, and other diseases. in addition, formation of isoasp is a major concern in protein pharmaceuticals, as it may lead to aggregation or activity loss. the scope and significance of isoasp have, up t ... | 2010 | 20712325 |
| peroxisomal plant 3-ketoacyl-coa thiolase structure and activity are regulated by a sensitive redox switch. | the breakdown of fatty acids, performed by the beta-oxidation cycle, is crucial for plant germination and sustainability. beta-oxidation involves four enzymatic reactions. the final step, in which a two-carbon unit is cleaved from the fatty acid, is performed by a 3-ketoacyl-coa thiolase (kat). the shortened fatty acid may then pass through the cycle again (until reaching acetoacetyl-coa) or be directed to a different cellular function. crystal structures of kat from arabidopsis thaliana and hel ... | 2010 | 20463027 |
| biological functions and biogenesis of secreted bacterial outer membrane vesicles. | gram-negative bacteria produce outer membrane vesicles (omvs) that contain biologically active proteins and perform diverse biological processes. unlike other secretion mechanisms, omvs enable bacteria to secrete insoluble molecules in addition to and in complex with soluble material. omvs allow enzymes to reach distant targets in a concentrated, protected, and targeted form. omvs also play roles in bacterial survival: their production is a bacterial stress response and important for nutrient ac ... | 2010 | 20825345 |
| microbial environment affects innate immunity in two closely related earthworm species eisenia andrei and eisenia fetida. | survival of earthworms in the environment depends on their ability to recognize and eliminate potential pathogens. this work is aimed to compare the innate defense mechanisms of two closely related earthworm species, eisenia andrei and eisenia fetida, that inhabit substantially different ecological niches. while e. andrei lives in a compost and manure, e. fetida can be found in the litter layer in forests. therefore, the influence of environment-specific microbiota on the immune response of both ... | 2013 | 24223917 |
| genetic detection of pseudomonas spp. in commercial amazonian fish. | brazilian freshwater fish caught from large drainages like the river amazon represent a million ton market in expansion, which is of enormous importance for export to other continents as exotic seafood. a guarantee of bacteriological safety is required for international exports that comprise a set of different bacteria but not any pseudomonas. however, diarrhoea, infections and even septicaemia caused by some pseudomonas species have been reported, especially in immune-depressed patients. in thi ... | 2013 | 24065035 |
| the influence of external factors on bacteriophages--review. | the ability of bacteriophages to survive under unfavorable conditions is highly diversified. we summarize the influence of different external physical and chemical factors, such as temperature, acidity, and ions, on phage persistence. the relationships between a phage's morphology and its survival abilities suggested by some authors are also discussed. a better understanding of the complex problem of phage sensitivity to external factors may be useful not only for those interested in pharmaceuti ... | 2011 | 21625877 |
| microbial d-xylonate production. | d-xylonic acid is a versatile platform chemical with reported applications as complexing agent or chelator, in dispersal of concrete, and as a precursor for compounds such as co-polyamides, polyesters, hydrogels and 1,2,4-butanetriol. with increasing glucose prices, d-xylonic acid may provide a cheap, non-food derived alternative for gluconic acid, which is widely used (about 80 kton/year) in pharmaceuticals, food products, solvents, adhesives, dyes, paints and polishes. large-scale production h ... | 2012 | 22875400 |
| co-culture with listeria monocytogenes within a dual-species biofilm community strongly increases resistance of pseudomonas putida to benzalkonium chloride. | biofilm formation is a phenomenon occurring almost wherever microorganisms and surfaces exist in close proximity. this study aimed to evaluate the possible influence of bacterial interactions on the ability of listeria monocytogenes and pseudomonas putida to develop a dual-species biofilm community on stainless steel (ss), as well as on the subsequent resistance of their sessile cells to benzalkonium chloride (bc) used in inadequate (sub-lethal) concentration (50 ppm). the possible progressive a ... | 2013 | 24130873 |
| novel staphylococcal glycosyltransferases sdga and sdgb mediate immunogenicity and protection of virulence-associated cell wall proteins. | infection of host tissues by staphylococcus aureus and s. epidermidis requires an unusual family of staphylococcal adhesive proteins that contain long stretches of serine-aspartate dipeptide-repeats (sdr). the prototype member of this family is clumping factor a (clfa), a key virulence factor that mediates adhesion to host tissues by binding to extracellular matrix proteins such as fibrinogen. however, the biological siginificance of the sdr-domain and its implication for pathogenesis remain poo ... | 2013 | 24130480 |
| prevention and treatment of biofilms by hybrid- and nanotechnologies. | bacteria growing as adherent biofilms are difficult to treat and frequently develop resistance to antimicrobial agents. to counter biofilms, various approaches, including prevention of bacterial surface adherence, application of device applicators, and assimilation of antimicrobials in targeted drug delivery machinery, have been utilized. these methods are also combined to achieve synergistic bacterial killing. this review discusses various multimodal technologies, presents general concepts, and ... | 2013 | 23946652 |
| examining the fish microbiome: vertebrate-derived bacteria as an environmental niche for the discovery of unique marine natural products. | historically, marine invertebrates have been a prolific source of unique natural products, with a diverse array of biological activities. recent studies of invertebrate-associated microbial communities are revealing microorganisms as the true producers of many of these compounds. inspired by the human microbiome project, which has highlighted the human intestine as a unique microenvironment in terms of microbial diversity, we elected to examine the bacterial communities of fish intestines (which ... | 2012 | 22574119 |
| differential biofilm formation and chemical disinfection resistance of sessile cells of listeria monocytogenes strains under monospecies and dual-species (with salmonella enterica) conditions. | this study aimed to investigate the possible influence of bacterial intra- and interspecies interactions on the ability of listeria monocytogenes and salmonella enterica to develop mixed-culture biofilms on an abiotic substratum, as well as on the subsequent resistance of sessile cells to chemical disinfection. initially, three strains from each species were selected and left to attach and form biofilms on stainless steel (ss) coupons incubated at 15°c for 144 h, in periodically renewable trypto ... | 2012 | 22307304 |
| engineering mammalian mucin-type o-glycosylation in plants. | mucin-type o-glycosylation is an important post-translational modification that confers a variety of biological properties and functions to proteins. this post-translational modification has a particularly complex and differentially regulated biosynthesis rendering prediction and control of where o-glycans are attached to proteins, and which structures are formed, difficult. because plants are devoid of galnac-type o-glycosylation, we have assessed requirements for establishing human galnac o-gl ... | 2012 | 22334671 |
| interstrain interactions between bacteria isolated from vacuum-packaged refrigerated beef. | the formation of bacterial spoilage communities in food is influenced by both extrinsic and intrinsic environmental factors. although many reports describe how these factors affect bacterial growth, much less is known about interactions among bacteria, which may influence community structure. this study investigated interactions among representative species of bacteria isolated from vacuum-packaged (vp) beef. thirty-nine effectors and 20 target isolates were selected, representing 10 bacterial g ... | 2015 | 25662972 |
| gcsr, a tyrr-like enhancer-binding protein, regulates expression of the glycine cleavage system in pseudomonas aeruginosa pao1. | glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (gcs). our lab previously identified the transcriptional regulator gcsr (pa2449) as being required for catabolism of glycine in the opportunistic pathogen pseudomonas aeruginosa pao1. in an effort to clarify and have an overall better understanding of the role of gcsr ... | 2016 | 27303730 |
| light chain-associated amyloid deposits comprised of a novel kappa constant domain. | light chain-associated amyloidosis is characterized by the deposition as fibrils of monoclonal light chain-related components consisting predominately of the variable domain (vl) or the vl plus up to approximately 60 residues of the constant domain (cl). here, we describe a patient (designated bif) with light chain-associated amyloidosis and kappa bence jones proteinuria in whom, notably, >80% of the amyloid deposits were comprised of cl-related material. the extracted amyloid protein consisted ... | 1998 | 9689117 |
| estimating bacterial growth parameters by means of detection times. | we developed a new numerical method to estimate bacterial growth parameters by means of detection times generated by different initial counts. the observed detection times are subjected to a transformation involving the (unknown) maximum specific growth rate and the (known) ratios between the different inoculum sizes and the constant detectable level of counts. we present an analysis of variance (anova) protocol based on a theoretical result according to which, if the specific rate used for the ... | 1999 | 9925608 |
| characterization of the pseudomonas aeruginosa metalloendopeptidase, mep72, a member of the vfr regulon. | pseudomonas aeruginosa vfr (the virulence factor regulator) enhances p. aeruginosa virulence by positively regulating the expression of numerous virulence genes. a previous microarray analysis identified numerous genes positively regulated by vfr in strain pak, including the yet uncharacterized pa2782 and pa2783. | 2013 | 24279383 |
| quantification of yeast and bacterial gene transcripts in retail cheeses by reverse transcription-quantitative pcr. | the cheese microbiota contributes to a large extent to the development of the typical color, flavor, and texture of the final product. its composition is not well defined in most cases and varies from one cheese to another. the aim of the present study was to establish procedures for gene transcript quantification in cheeses by reverse transcription-quantitative pcr. total rna was extracted from five smear-ripened cheeses purchased on the retail market, using a method that does not involve prior ... | 2013 | 23124230 |
| structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism. | the alginate-assimilating bacterium, sphingomonas sp. strain a1, degrades the polysaccharides to monosaccharides through four alginate lyase reactions. the resultant monosaccharide, which is nonenzymatically converted to 4-deoxy-l-erythro-5-hexoseulose uronate (deh), is further metabolized to 2-keto-3-deoxy-d-gluconate by nadph-dependent reductase a1-r in the short-chain dehydrogenase/reductase (sdr) family. a1-r-deficient cells produced another deh reductase, designated a1-r', with a preference ... | 2014 | 25288804 |
| whole genome and transcriptome analyses of environmental antibiotic sensitive and multi-resistant pseudomonas aeruginosa isolates exposed to waste water and tap water. | the fitness of sensitive and resistant pseudomonas aeruginosa in different aquatic environments depends on genetic capacities and transcriptional regulation. therefore, an antibiotic-sensitive isolate pa30 and a multi-resistant isolate pa49 originating from waste waters were compared via whole genome and transcriptome illumina sequencing after exposure to municipal waste water and tap water. a number of different genomic islands (e.g. pagis, papis) were identified in the two environmental isolat ... | 2014 | 25186059 |