Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [reiterant contaminations by "pseudomonas aeruginosa" on culture cell lines in a hospital virology service (author's transl)]. | the selection of antibiotic resistant pseudomonas aeruginosa strains by an indiscriminate administration of antibiotics has been observed. the consequences induced by ps. aeruginosa on the tissue culture cell lines (both primary and continuos) are harmful, velding a sharp ph drop from a slight initial increase, followed by the destruction of the cell layer. sometimes the cells succeed in growing up for some passages since antibiotics are usually present in the growth medium. tissue culture conta ... | 1978 | 111632 |
| regions of the lipopolysaccharide of pseudomonas aeruginosa essential for antitumor and interferon-inducing activities. | resistance against ascites tumor development and interferon-inducing activity were demonstrated in lipopolysaccharide derived from the protein-lipopolysaccharide complex obtained from an autolysate of pseudomonas aeruginosa. lipid a obtained from the lipopolysaccharide was sufficient to induce interferon in vitro but no antitumor activity was found if lipid a or the polysaccharide derived from lipopolysaccharide was injected into the animal. chemical modification of the polysaccharide portion or ... | 1979 | 111929 |
| genetic mapping of the region c of the bacteriophage g101 (pseudomonas aeruginosa). | morphological mutants of the c type of the bacteriophage g101 (pseudomonas aeruginosa) were isolated after mutagenesis with hydroxylamine. complementation analysis of 27 c mutants showed that the c region is formed by at least two genes. two types of c mutants were obtained. one of them (ci26) behaves analogously to a mutant in the gene controlling the synthesis of the repressor of phage lambda. the second type of the c mutants (cii1, cii18) specifies a gene having probably an auxiliary function ... | 1979 | 112014 |
| serological typing of pseudomonas aeruginosa. | 1979 | 112052 | |
| production of exoenzyme s during pseudomonas aeruginosa infections of burned mice. | antisera which distinguished between pseudomonas aeruginosa exoenzyme s and toxin a neutralized the adenosine diphosphate ribosyl transferase activity of the homologous, but not the heterologous, enzyme. skin extracts and sera from burned mice infected with the exoenzyme s-producing strain p. aeruginosa 388 contained adenosine diphosphate ribosyl transferase activity that was not found in skin extracts or sera from uninfected mice. on the basis of immunological reactivity and enzymatic propertie ... | 1979 | 112058 |
| experimental bacterial keratitis in neutropenic guinea pigs: polymorphonuclear leukocytes in corneal host defense. | quantitative techniques were used to determine the relative concentrations of viable bacteria and polymorphonuclear leukocytes (pmns) in the corneas of neutropenic and non-neutropenic guinea pigs with experimental bacterial keratitis induced with three strains of pseudomonas aeruginosa. neutropenia was produced by whole-body x-irradiation 1 week before infection. significantly greater numbers of bacteria were present in the cornea of neutropenic animals 48 h after infection than were present in ... | 1979 | 112062 |
| [studies on the pseudomonas aeruginosa infections--frequency, antibiotics sensitivity, oep-ha titer (author's transl)]. | 1979 | 112204 | |
| transfer of plasmid-mediated antibiotic resistance in strains of pseudomonas aeruginosa isolated in auckland. | of 422 clinical strains of pseudomonas aeruginosa, 23 (5.5%) were resistant to gentamicin; 19 were also resistant to tobramycin and sisomycin, and one was resistant to tobramycin, sisomycin and amikacin. of the gentamicin-resistant strains, 20 were also resistant to kanamycin. sixteen strains with a high level of resistance to gentamicin (mic greater than 160 microgram/ml) transferred all their resistance determinants to recipient strains of p. aeruginosa and four transferred some resistance det ... | 1979 | 112254 |
| sensitivity of neisseria gonorrhoeae to partially purified r-type pyocines and a possible approach to epidemiological typing. | strains of neisseria gonorrhoeae from a variety of sources were examined for sensitivity to 11 partially purified r-type pyocines from pseudomonas aeruginosa. selective inhibition of gonococci by pyocines of kageyama groups r1 and r5 was observed. "matched isolates", those from consorts or different body sites of individual patients, usually had very similar pyocine-sensitivity patterns and identical sensitivities to five antibiotics tested. this study included local isolates, strains from diver ... | 1979 | 112255 |
| proposal of an international standard for the infraspecific serologic classification of pseudomonas aeruginosa. | 1979 | 112272 | |
| gram-negative germs infections in infancy. | the incidence of systemic or local infections due to gram-negative bacilli in an infant ward from september 1969 to december 1976 was 7.9%. the 29.34% were septicemia, most of them as epidemic outbreaks caused by pseudomonas aeruginosa, klebsiella-enterobacter and serratia marcescens. two facts are to be emphasized: an almost complete disappearance of systemic infections with pseudomonas starting from 1972, and the global predominance of the group klebsiella-enterobacter, particularly evident fr ... | 1979 | 112565 |
| [relationship between pyocine and temperate phage of pseudomonas aeruginosa (author's transl)]. | 1979 | 112650 | |
| [the antibiogram in pseudomonas aeruginosa infections]. | 1978 | 112746 | |
| [virulence and the antigens of pseudomonas aeruginosa]. | 1979 | 112806 | |
| cell wall characteristics of pseudomonas aeruginosa and its carbenicillin-induced l-form. | l-forms of pseudomonas aeruginosa were induced and cultured on a medium supplemented with carbenicillin. morphological studies of the passaged variant revealed the presence of a triple-layered cell wall similar to that found in the parent species. furthermore, the l-form was found to be more susceptible to gentamicin, kanamycin, tetracycline and colistin sulphate. chemical analysis of the lipopolysaccharide fraction showed a difference in phosphorus content, and changes in cell wall envelope fat ... | 1978 | 112813 |
| in vitro comparison of rosamicin and erythromycin against urinary tract pathogens. | the in vitro activity of rosamicin and erythromycin was compared at various ph values against 311 strains of bacteria representing common urinary tract pathogens. alkalinization of the media consistently and significantly increased the antibacterial activity of rosamicin against all of the organisms tested. this was also true for erythromycin except when tested against strains of proteus. at ph 8, rosamicin was two- to sixfold more active than erythromycin against enterobacteriaceae. the activit ... | 1979 | 112920 |
| epidemiological markers for pseudomonas aeruginosa. vii. nosocomial occurrence in urological ward. comparison of two phage typing sets, pyocine typing, and serogrouping. | a comparison of two pyocine typing methods with serogrouping, and two phage typing sets has been made. in one instance, a contaminated bladder irrigation fluid of 0.05% silver nitrate solution caused a series of urinary tract infections. by all systems, fewer of the presentstrains were typable than usual. among the phage typing sets the one developed by lindberg et al. typed only 66% whereas that selected by bergan typed 83%, both less than observed previously. the set of lindberg et al. also re ... | 1979 | 112947 |
| pseudomonas corneal ulcer. the causative role of contaminated eye cosmetics. | the clinical significance of contaminated ocular cosmetics is illustrated by the case of a 47-year-old woman in whom a pseudomonas corneal ulcer developed immediately after she sustained minor corneal trauma with a mascara applicator. pseudomonas aeruginosa was cultured from the corneal ulcer and the mascara. in addition to the causative role in acute corneal ulcers, contaminated eye cosmetics contribute to chronic external eye infections. retail eye cosmetics are typically free of contamination ... | 1979 | 112953 |
| transformation of pseudomonas aeruginosa strain pao with bacteriophage and plasmid dna. | a procedure has been developed which allows transformation of p. aeruginosa strain pao with plasmid and bacteriophage dna at a frequency of 10(-6) per recipient cell. the method is similar in outline to that developed for escherichia coli. it involves growing the recipient cells to 3-5 x 10(8) per ml in nutrient broth, washing the cells with 0.1 m mgcl2, resuspending in 0.175 m cacl2 for 20 min, exposing to dna for 1 h and then heat pulsing at 42 degrees c for 1 min. some plasmid markers are exp ... | 1978 | 112956 |
| catabolism of taurine in pseudomonas aeruginosa. | cell-free extracts of taurine-grown pseudomonas aeruginosa catalyze the transamination of taurine and pyruvate resulting in the formation of l-alanine and sulfoacetaldehyde. the enzyme responsible for this activity has been partially purified in order to demonstrate its participation in a pathway of taurine degradation. ethyl methane sulfonate treatment of ps. aeruginosa yielded a mutant deficient in taurine transaminase and incapable of growing on taurine indicating that the enzyme is of physio ... | 1979 | 113035 |
| transfer and integration of chromosomal genes from pseudomonas glycinea into pseudomonas aeruginosa. | the plasmids fp2 and r68.45 were shown to function as chromosome-mobilizing plasmids in a series of interspecific crosses between the phytopathogen pseudomonas glycinea and the human pathogen pseudomonas aeruginosa. at least four of seven loci tested were transferred from p. glycinea donors to p. aeruginosa auxotrophic recipients. transductional analysis indicates that a leu+ locus of the p. glycinea chromosome transferred is stably integrated into the p. aeruginosa chromosome. | 1979 | 113066 |
| the effect of phenazine methosulfate-ascorbate on bacterial active transport and adenosine triphosphate formation: inhibition of pseudomonas aeruginosa and stimulation of escherichia coli. | the artificial electron-donor system, phenazine methosulfate (pms) ascorbate, inhibited active transport of glucose by pseudomonas aeruginosa irrespective of whether the incubation systems were in air, flushed with oxygen, or gassed with nitrogen under anaerobic denitrifying conditions. active transport of glucose by p. aeruginosa was also inhibited by reduced 5-n-methyl-phenazonium-3-sulfonate, a membrane-impermeable electron donor. pms-ascorbate caused rapid depletion of intracellular adenosin ... | 1979 | 113071 |
| in vitro efficacy of bay k 4999, a new ureido-penicillin, in combination with aminoglycosides against pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae and proteus strains. | the in vitro effects of bay k 4999 in combination with gentamicin, tobramycin, amikacin sisomicin and netilmicin in bacteriostatic (mic) and bactericidal (mbc) concentrations were compared using the checkerboard dilution technique against 20 different strains of pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae and indole-positive-negative proteus species. on average 63% of bay k 4999-aminoglycoside (ag) combinations inhibited pseudomonas, proteus and klebsiella strains additively ... | 1979 | 113178 |
| transport systems for branched-chain amino acids in pseudomonas aeruginosa. | the cells of pseudomonas aeruginosa showed high activity for leucine transport in the absence of na+, giving a km value of 0.34 microm. in the presence of na+, however, two km values, 0.37 microm (liv-i system) and 7.6 microm (liv-ii system), were obtained. the former system seemed to serve not only for the entry of leucine, isoleucine, and valine, but also for that of alanine and threonine, although less effectively. however, the liv-ii system served for the entry of branched-chain amino acids ... | 1979 | 113383 |
| genetic and physiological characterization of pseudomonas aeruginosa mutants affected in the catabolic ornithine carbamoyltransferase. | in pseudomonas aeruginosa arginine can be degraded by the arginine "dihydrolase" system, consisting of arginine deiminase, catabolic ornithine carbamoyltransferase, and carbamate kinase. mutants of p. aeruginosa strain pao affected in the structural gene (arcb) of the catabolic ornithine carbamoyltransferase were isolated. firt, and argf mutation (i.e., a block in the anabolic ornithine carbamoyltransferase) was suppressed specifically by a mutationally altered catabolic ornithine carbamoyltrans ... | 1979 | 113384 |
| transposable plasmid deoxyribonucleic acid sequence in pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents. | a 9.1 x 10(6)-dalton transposable deoxyribonucleic acid sequence resides within pseudomonas aeruginosa plasmid r1033 and mediates resistance to gentamicin, streptomycin, sulfamethoxazole, chloramphenicol, and mercuric chloride. transposability was demonstrated in escherichia coli when this sequence, designated tn1696, excised from r1033 and integrated into plasmid pmb8. excision and insertion of tn1696 occurred independently of the host rec phenotype and may involve the 140-base pair, inverted d ... | 1979 | 113388 |
| effect of repair deficiency and r plasmids on spontaneous and radiation-induced mutability in pseudomonas aeruginosa. | the effect of r plasmids on spontaneous and radiation (ultraviolet and gamma)-induced mutability in pseudomonas aeruginosa was studied in strains containing the radiation-sensitive markers pola3 or rec-2 and the revertable auxotrophic markers hiso27 and trpb1. in the absence of an r plasmid, the radiation-induced mutability was dependent on the reca+ genotype and independent of the pola+ genotype, whereas spontaneous mutability was similar in all genetic backgrounds. r plasmids ppl1, r2, and pmg ... | 1979 | 113392 |
| membrane-bound d-gluconate dehydrogenase from pseudomonas aeruginosa. its kinetic properties and a reconstitution of gluconate oxidase. | 1979 | 113395 | |
| [variability of the enzyme properties of the p-xylene oxidizing 2x strain of pseudomonas aeruginosa]. | 1979 | 113442 | |
| synergy between carbenicillin and an aminoglycoside (gentamicin or tobramycin) against pseudomonas aeruginosa isolated from patients with endocarditis and sensitivity of isolates to normal human serum. | isolates from the blood of 30 patients with endocarditis due to pseudomonas aeruginosa were tested for synergy between carbenicillin and an aminoglycoside, either gentamicin or tobramycin, by in vitro checkerboard methods in modified (cation-supplemented) mueller-hinton broth. twenty-five of the 30 isolates were affected synergistically. whether given low (2.5--5 mg/kg) or high (8 mg/kg) doses of aminoglycoside along with 30 g of carbenicillin daily, all of the five patients infected with pseudo ... | 1979 | 113468 |
| effect of piperacillin on d-alanine carboxypeptidase activities from pseudomonas aeruginosa. | membrane-bound d-alanine carboxypeptidase activity from pseudomonas aeruginosa is very sensitive to inhibition by piperacillin. | 1979 | 113486 |
| effect of bacteriophage c5 on ultraviolet light survival in pseudomonas aeruginosa. | bacteriophage c5 of pseudomonas aeruginosa is able to reactivate ultraviolet (u.v.)-irradiated phage e79 in coinfection experiments and decrease the u.v.-sensitivity of a host-cell reactivation deficient mutant. these properties suggest that phage c5 has a gene(s) which is involved in the repair of u.v.-damaged dna. the isolation of two u.v.-sensitive mutants of c5 supports this hypothesis. | 1979 | 113492 |
| [an epidemic of pseudomonas keratitis due to permanent-wear contact lenses (author's transl)]. | in 1977 thirty-three patients (35 eyes) with permanent-wear contact lenses presented with keratitis in varying degrees of severity. among the nine cases investigated bacteriologically (including some contact lenses) three showed no organisms, while six were found to have pseudomonas aeruginosa. the contact lenses all came from the same optician, where shortly beforehand the patients had had their lenses either replaced or checked. the inspection of the optical establishment revealed faultless ap ... | 1979 | 113611 |
| pmg7-mediated restriction of pseudomonas aeruginosa phage dnas is determined by a class ii restriction endonuclease. | 1979 | 113797 | |
| iga and igg antibodies against surface antigens of pseudomonas aeruginosa in sputum and serum from patients with cystic fibrosis. | eleven cystic fibrosis (cf) patients chronically infected in the lungs with mucoid pseudomonas aeruginosa and presenting multiple precipitins in serum against this bacterium (cf + p) and 10 cf patients without p. aeruginosa infection (cf-p) had their serum and sputum sol phase specimens examined for antibodies of the iga and igg classes against surface antigens of p. aeruginosa by means of an indirect immunofluorescence technique. both the iga and igg antibody titres demonstrated in serum and sp ... | 1979 | 113981 |
| a pseudomonas pyocin typing method applicable to the clinical laboratory. | a new pyocin typing method for the identification of specific strains of pseudomonas aeruginosa is described. the method involves a bilayer agar technique in which the pyocin produced by the organism diffuses from the top agar layer number 2 through a filter paper into the bottom agar layer number 1. the filter paper permits the removal of agar layer number 2 and exposes layer number 1 which is then streaked with the indicator strains. pyocin typing of fifty clinical isolates using both the bi-l ... | 1979 | 114048 |
| effect of antibiotics on mucoid strains of pseudomonas aeruginosa. | a study of the susceptibility to 18 antibiotics has been performed on 47 mucoid strains comparatively to 71 fried-egg strains of pseudomonas aeruginosa. the minimal inhibitory concentration was determinated by agar dilution method. on the whole, the mucoid strains were more sensitive to antibiotics than the fried-egg strains. the more effective antibiotics were carbenicillins, aminosids, colistin and tetracyclines. however, this drug susceptibility of mucoid strains were heterogeneous. the resul ... | 1979 | 114082 |
| [comparison of bactericidal effects of four aminoglycoside antibiotics: amikacin, gentamicin, kanamycin and tobramycin (author's transl)]. | the authors have studied on 20 bacterial strains (5 pseudomonas aeruginosa, 5 escherichia coli, 5 klebsiella and 5 serratia) the bactericidal kinetics of 4 aminoglycoside antibiotics: amikacin, gentamicin, kanamycin and tobramycin. the antibiotic concentrations used for this work were 1.5 times the mic for each strain previously measured in a liquid medium. the action of the aminoglycoside antibiotics shows three phases. the third phase describes a part of less susceptible bacterial population. ... | 1979 | 114083 |
| [pseudomonas aeruginosa plasmids that control streptomycin resistance]. | wide distribution of streptomycin resistance determinants (83 per cent) among the resistance plasmids of the clinical strains of ps. aeruginosa isolated in several clinics of 2 towns was found. nine plasmids determining resistance to this antibiotic, as well as some other antibiotics, sulfanilamides, metallic ions, hydroxyanions and uv radiation were studied. the frequency of the conjugation transfer in these plasmids was different, i.e. from 10(1) to 10(6). they belonged to the following incomp ... | 1979 | 114103 |
| diffusion disk susceptibility testing with cefotaxime. | the diffusion disk susceptibility of staphylococcus aureus, enterobacteriaceae, and pseudomonas aeruginosa to cefotaxime was determined. a 30-mug disk provided data for the susceptibility of p. aeruginosa, but yielded extremely large zones of inhibition against enterobacteriaceae. a 5-mug disk seemed to provide the most useful susceptibility data for s. aureus and enterobacteriaceae. | 1979 | 114106 |
| activity of beta-lactam antibiotics against pseudomonas aeruginosa carrying r plasmids determining different beta-lactamases. | azlocillin, carbenicillin, cefsulodin, mezlocillin, nocardicin a, piperacillin, pirbenicillin, sulbenicillin, and ticarcillin, but not hr756, showed reduced activity against pseudomonas aeruginosa strains producing seven types of beta-lactamase. | 1979 | 114107 |
| transfer of r factors to and between genetically marked sublines of rhizobium japonicum. | plasmids r1822 and prd1 of the p-1 incompatibility group, for which rhizobium japonicum had not previously been shown to serve as host, were introduced into a strain of r. japonicum. acquisition of r68 and r68.45 plasmids by this rhizobium was equivocal. transfer of r1822 from pseudomonas aeruginosa and of prd1 from escherichia coli to r. japonicum was unambiguous, because the exconjugants subsequently cotransferred the three r-factor resistance determinants (kanamycin, tetracycline, and penicil ... | 1979 | 114109 |
| effects of hyperbaric oxygen upon s. aureus, ps. aeruginosa and c. albicans. | numerous in vitro investigations have reported that prolonged, continuous hyperbaric oxygen (ohp) exposure to certain bacteria and yeast is bacteriostatic. while it is tempting to attribute the lower infection rates reported in ohp-treated patients to this bacteriostatic effect, the duration and intensity of ohp exposure in these experimental studies exceeds that of normal therapeutic use. this study was designed to investigate the effects of human ohp treatment protocols upon the in vitro growt ... | 1979 | 114160 |
| biosynthesis of proline in pseudomonas aeruginosa. properties of gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase. | gamma-glutamyl phosphate reductase, the second enzyme of proline biosynthesis, catalyses the formation of l-glutamic acid 5-semialdehyde from gamma-glutamyl phosphate with nad(p)h as cofactor. it was purified 150-fold from crude extracts of pseudomonas aeruginosa pao 1 by deae-cellulose chromatography and hydroxyapatite adsorption chromatography. the partially purified preparation, when assayed in the reverse of the biosynthetic direction, utilized l-1-pyrroline-5-carboxylic acid as substrate an ... | 1979 | 114173 |
| identification of the protein producing transmembrane diffusion pores in the outer membrane of pseudomonas aeruginosa pa01. | the outer membrane of pseudomonas aeruginosa pa01 is permeable to saccharides of molecular weights lower than about 6000. triton x-100/edta-soluble outer membrane proteins were fractionated by ion-exchange chromatography in the presence of triton x-100 and edta, and the protein contents of the various fractions analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. each of the major protein bands present in the triton x-100/edta soluble outer membrane was separated from one anot ... | 1979 | 114220 |
| insignificance of colonic bacteria in the sputum of patients in a new icu. | over a 12-month period, 27% of patients in a new icu grew bacterial pathogens from sputum or tracheal cultures. the commonest isolates were pseudomonas aeruginosa and klebsiella species. endotracheal intubation, the length of time intubated, and antimicrobial therapy all predisposed to the isolation of organisms from sputum. no patient developed a gram-negative pneumonia, and there was no case of septicemia associated with a positive sputum culture. the presence of epithelial or pus cells in spu ... | 1979 | 114356 |
| comparative studies of penicillin-binding proteins in pseudomonas aeruginosa and escherichia coli. | penicillin-binding proteins in pseudomonas aeruginosa were compared with those of escherichia coli. these in p. aeruginosa were found exclusively in the cytoplasmic membrane fraction (fraction soluble in sodium n-lauroyl sarcosinate). sodium dodecyl sulfate/acrylamide gel electrophoresis of the proteins bound to [14c]penicillin g resulted in the separation of six major bands and several minor bands. the proteins in these bands are referred to as proteins 1a, 1b, 2, 3, 4 and 5 in order of increas ... | 1979 | 114388 |
| modification of the cell surface with neuraminidase increases the sensitivities of cells to diphtheria toxin and pseudomonas aeruginosa exotoxin. | 1979 | 114400 | |
| differences of chemical structures of pseudomonas aeruginosa lipopolysaccharide essential for adjuvanticity and antitumor and interferon-inducing activities. | 1979 | 114416 | |
| photoaffinity labelling of a tyrosine chemoreceptor in pseudomonas aeruginosa. | 1979 | 114420 | |
| [biological characteristics of various strains of pseudomonas aeruginosa isolated from human pathological matter]. | according to the higher frequency of isolation of bacterial strains belonging to the genus pseudomonas from various human phatologic materials, in was seemed interesting to study the biological characteristics of these bacteria, which are the most virulent and pathogenic gram-microorganism. in the present work the results of cultural, biochemical, cytochemical and immunological tests are reported. in vivo tests were also carried out, using rabbits inoculated with whole cells or their products. | 1979 | 114444 |
| growth of pseudomonas aeruginosa in normal and burned skin extract: role of extracellular proteases. | growth curves and mean generation times (mgt) were determined for pseudomonas aeruginosa strain m-2 (protease +) and strain pa-103 (protease +/-) in burned skin extract (bse) and in normal skin extract (nse). strain m-2 grew on nse or bse with an mgt of 30 min. strain pa-103 grew in nse at a similar mgt; however, pa-103 in bse had a mgt of 65 min. when protease was added to bse, pa-103 grew as rapidly as m-2. when ammonium sulfate was added to inhibit protease production, the mgt of m-2 slowed t ... | 1979 | 114486 |
| secretion of phospholipase c by pseudomonas aeruginosa. | the conditions necessary for the secretion of phospholipase c (phosphatidylcholine cholinephosphohydrolase) by pseudomonas aeruginosa were studied. enzyme secretion by washed cell suspensions required a carbon source and ammonium, potassium, and calcium ions. the calcium requirement could be substituted by magnesium and strontium but not by copper, manganese, cobalt, or zinc. during growth in liquid medium, cells secreted phospholipase c during late logarithmic and early stationary phases. secre ... | 1979 | 114487 |
| protection against hemorrhagic pneumonia of mink by pseudomonas aeruginosa multicomponent vaccine. | an attempt to prevent epidemics of hemorrhagic pneumonia in mink due to pseudomonas aeruginosa was made in the course of epidemics with injection of the multicomponent vaccine consisting of common protective antigen (oep) of p. aeruginosa mixed with toxoids of protease and elastase of the bacillus. enzootics of hemorrhagic pneumonia, due to p. aeruginosa serotype 8, broke out from august to october 1977 in a total of 13 sheds of 3 farms (a, b and c) which were located in the northeast area of ho ... | 1979 | 114699 |
| pseudomonas aeruginosa keratitis treated with ticarcillin and tobramycin. | a corneal ulcer, infected with pseudomonas aeruginosa and complicated by conjunctivitis and endophthalmitis, was treated successfully with systemic administration of ticarcillin and topical application of tobramycin. it is unlikely that carbenicillin, to which the organism was much less sensitive, would have attained sufficient tissue levels to control the infection. | 1979 | 114740 |
| precipitating antibodies against pseudomonas aeruginosa in serum from dialysis patients. | hemodialysate in our facility was found contaminated with pseudomonas aeruginosa. by means of crossed immunoelectrophoresis, series of serum samples from 20 patients in regular dialysis treatment have been investigated for development of precipitating antibodies against p. aeruginosa during dialysis treatment. in 6 patients antibacterial antibodies occurred during the observation period but were, in all 6 occasions, in close relation to acute intercurrent bacterial infections and not related to ... | 1979 | 114868 |
| experimental infections with staphylococcus aureus, streptococcus sanguis, pseudomonas aeruginosa, and bacteroides fragilis in the jaws of dogs. | 1979 | 114906 | |
| paeexo ix: a unique deoxyribonuclease from pseudomonas aeruginosa active in the presence of edta. | a new deoxyribonuclease, paeexo ix, has been purified to electrophoretic homogeneity from extracts of pseudomonas aeruginosa strain pao. this enzyme, which is active in the presence of edta, is equally efficient in hydrolyzing native and heart-denatured dna to acid-s-luble products. the enzyme is partially or totally inhibited by the presence of several divalent cations. the active protein has a molecular weight of 1.6 +/- 0.1 x 10(5) and is composed of two nonidentical polypeptides with molecul ... | 1979 | 114985 |
| studies on egg disinfection. | various concentrations of alkyldimethylbenzyl ammonium chloride (qac), na2co3, and ethylenediaminetetracetic acid (edta) were tested for antimicrobial activity singly and in combination against escherichia coli, arizona hinshawii, and pseudomonas aeruginosa. bactericidal activity of the reagents were evaluated in embryonating eggs, trypticase soy broth, and a medium containing lecithin. toxicity of the chemicals was assayed in embryonating eggs. an appraisal was made of an egg-washing solution c ... | 1979 | 114994 |
| effects of pseudomonas toxin a, diphtheria toxin, and cholera toxin on electrical characteristics of turtle bladder. | rapidly developing changes in the short-circuiting current (isc), conductance (g), and potential (pd) of turtle bladders in na-rich or na-free media are seen after the mucosal addition, at 10 nm, of each of three toxins that contain adp-ribosylation activity: pseudomonas aeruginosa toxin a, diphtheria toxin, and cholera toxin. toxin a irreversibility decreased the isc, pd, and g of bladders in na-rich media and the isc and pd of bladders in na-free media. diphtheria or cholera toxin reversibly i ... | 1979 | 115005 |
| [ultrastructural changes in the cells of a nonsynchronous pseudomonas aeruginosa culture occurring under the influence of chlorhexidine bigluconate]. | total preparations of p. aeruginosa (strain 65) were studied after contrastive treatment with 2% phosphato-tungstic acid and 2% uranyl acetate; ultrathin sections of bacteria treated with chlorhexidine bigluconate in various concentrations and fixied by the method of hoffmann et al. were also studied with the use of an electron microscope iem 100 v. structural and morphological changes depending on the concentration and the time of action of the antiseptic were discovered; these changes were man ... | 1979 | 115182 |
| [effect of oxacillin on the development of resistance of pseudomonas aeruginosa against carbenicillin (author's transl)]. | 1979 | 115477 | |
| global and effective synergism of amikacin, gentamicin or tobramycin when combined with carbenicillin against pseudomonas aeruginosa. | the authors describe a study to evaluate the synergistic effects of the combinations of amikacin-carbenicillin, gentamicin-carbenicillin and tobramycin-carbenicillin at the associated minimal bactericidal concentrations and at doses related to the serum levels reached by the drugs in the blood. | 1979 | 115732 |
| evaluation of a new polyvalent pseudomonas vaccine in respiratory infections. | a new polyvalent, cell wall extract, pseudomonas aeruginosa vaccine (pev-01), was evaluated by using a guinea pig model of experimental pseudomonas pneumonia. guinea pigs routinely developed fourfold rises in serum hemagglutinating pseudomonas antibodies after four vaccine injections given over 2 weeks. vaccinated animals survived an intratracheal pseudomonas challenge (1 x 10(8) colony-forming units) significantly better (13 of 14 survived) than did a control group (5 of 14 survived) (p less th ... | 1979 | 115786 |
| passage of pseudomonas aeruginosa in compromised mice. | there was no appreciable increase in the virulence of pseudomonas aeruginosa pao-1 after six passes through infections in mice. when strain pao-1 was passed through mice compromised with iron and methotrexate, the virulence of the passed bacteria increased for normal as well as compromised mice. bacteria harvested from intraperitoneal passage in compromised mice were more virulent than bacteria harvested from intrathoracic passage. these bacteria expressed 50% lethal dose values distinctive of t ... | 1979 | 115795 |
| the effect of triethylenetetramine dihydrochloride on the in vivo susceptibility of pseudomonas aeruginosa to gentamicin. | a chelating agent, triethylenetetramine dihydrochloride (trien dihydrochloride) increased the efficacy of gentamicin in vivo against a clinical isolate of p. aeruginosa, designated ps 15. mice which were inoculated with 10 x ld50 of ps 15 and treated with doses of 2 approximately 16 mg of gentamicin per kg per day all died. however, treatment with 8 mg of gentamicin per kg body weight per day plus 30 mg of trien dihydrochloride per day markedly reduced the mortality. the combined therapy also re ... | 1979 | 115822 |
| bacteriological studies with cefsulodin (cgp 7174/e), the first antipseudomonal cephalosporin. | the new cephalosporin, cefsulodin, has considerable antibacterial activity against pseudomonas aeruginosa. when 217 strains of ps. aeruginosa were tested against both azlocillin and cefsulodin, 26.3% were found to have the same minimal inhibitory concentration (mic); the mic for azlocillin was lower than that for cefsulodin in 16.6% of strains, but higher in 57.1%. 22 gentamicin-resistant strains were all susceptible to cefsulodin. biophotometer investigations demonstrate less bactericidal effec ... | 1979 | 115832 |
| transformation and transfection of pseudomonas aeruginosa: effects of metal ions. | the ability of different metal ions to promote transformation of pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid rp1 was examined. cacl2, mgcl2, and mncl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. only mgcl2 promoted transfection of p. aeruginosa by the linear deoxyribonucleic acid of phage f116. cacl2 was demonstrated to allow adsorption and entry into the cell of f116 deoxyribonucleic a ... | 1979 | 115840 |
| the complete amino acid sequence of the beta-subunit of protocatechuate 3,4-dioxygenase from pseudomonas aeruginosa. | the complete amono aicd sequence of the beta-subunit of protocatechuate 3,4-dioxygenase is presented. the beta-subunit contained 237 amino acid residues, 4 of which were methionines. accordingly, cyanogen bromide cleavage of the s-carboxymethylated beta-subunit produced five peptides. the sequences of these peptides were determined by analyses of the peptides obtained by tryptic, staphyloccal protease and thermolysin digestions. the alignment of the cyanogen bromide peptides was deduced by the u ... | 1979 | 115853 |
| isolation and characterization of major outer membrane proteins of pseudomonas aeruginosa strain pao with special reference to peptidoglycan-associated protein. | the outer membrane of pseudomonas aeruginosa pao contains six major proteins (proteins d, e, f, g,h, and i). two of them (protein f and protein h) were found to be retained by the peptidoglycan layer when cell envelopes were extracted with 2% sodium dodecyl sulfate (sds) solution at 35 degrees c. at higher temperature (greater than 55 degrees c), no proteins were retained by peptidoglycan. by making use of this property, purification of protein f and protein h was achieved. three other major out ... | 1979 | 115859 |
| a novel peptidoglycan-associated lipoprotein found in the cell envelope of pseudomonas aeruginosa and escherichia coli. | protein h, one of the major outer membrane proteins pseudomonas aeruginosa, shows an interesting interaction with the peptidoglycan layer of the cell. it is retained by peptidoglycan after extraction of the cell envelope with sds solution at 35 degrees c. a protein of the same molecular weight (21,000) as protein h was found in the peptidoglycan-associated fraction of escherichia coli k-12 prepared under the same conditions. this protein, designated here as protein 21k, was purified from the cel ... | 1979 | 115860 |
| enzyme-linked immunosorbent assay for pseudomonas aeruginosa exotoxin a. | an enzyme-linked immunosorbent assay (elisa) is described for pseudomonas aeruginosa exotoxin a. a double antibody sandwich method was used, employing polyvinyl microtiter plates as the solid phase, a primary coat of monospecific rabbit antitoxin serum, an outer layer composed of a horseradish peroxidase-sheep antitoxin immunoglobulin g conjugate, and an ortho-phenylene-diamine substrate. absorbance (optical density) of hydrolyzed end product was read spectrophotometrically at 492 nm. elisa dete ... | 1979 | 115899 |
| characteristics of pseudomonas aeruginosa in relation to laboratory-induced resistance to gentamicin. | pseudomonas aeruginosa strain c2 was habituated to gentamicin by serial passage in broth containing increasing concentrations of the antibiotic and up to 250 microgram/ml. the resistant progenies differed from the parent strain in antibiotic susceptibility to two other aminoglycosides, colonial morphology, lytic phage patterns, phage adsorption, and agglutination with the seven fisher's antisera. all the progenies failed to grow at 42 degrees c and oxidised glucose in o/f tubes after incubation ... | 1979 | 115904 |
| [an experimental study on pseudomonas osteomyelitis with special reference to the production of experimental osteomyelitis in mice (author's transl)]. | i) the author has successfully produced a model of experimental osteomyelitis caused by pseudomonas aeruginosa using the following procedure though such a demonstration has been said to be very difficult. after impregnation in a solution containing about 10(5) pseudomonas aeruginosa, 3 mm silk thread of no. 5 was dried under low-pressure atmosphere and then inserted into the metaphysis of right tibia of a mouse. this method can be produced experimental osteomyelitis in 100% of the animals. in th ... | 1979 | 115949 |
| inhibitory effect of cystic fibrosis serum on pseudomonas phagocytosis by rabbit and human alveolar macrophages. | this report presents experimental observations indicating the presence of an inhibitory activity in cystic fibrosis (cf) serum which impairs phagocytosis of pseudomonas aeruginosa by rabbit as well as human alveolar macrophages. of the 49 patient serum samples studied, 40 consistently showed greater than or equal to 60% inhibition, 3 showed no inhibition and 6 were in the range of 20-60% inhibition of pseudomonas phagocytosis. in parallel studies, the phagocytosis of s. aureus and s. marcescens ... | 1979 | 116188 |
| [use of a vaccine for prevention of pseudomonas aeruginosa infection of burn wounds in mice]. | 1979 | 116210 | |
| a collaborative study on a new quantitative suspension test, the in vitro test, for the evaluation of the bactericidal activity of chemical disinfectants. | in a collaborative study on a new quantitative suspension test for the evaluation of the bactericidal activity of chemical disinfectants, three laboratories performed the in vitro test on a phenol and an aldehyde standard in the critical use dilutions using staphylococcus aureus and pseudomonas aeruginosa as test organisms. the most striking finding was that the means of the germidical effect of one laboratory were significant lower than those of both others. nevertheless the dispersion of the r ... | 1979 | 116440 |
| [immunological study of the cellular components of pseudomonas aeruginosa. vi. the toxicity and protective properties of its mucus and its fractions]. | capsule-like mucus was obtained from two newly isolated mucoid strains of p. aeruginosa and then treated with ethanol. the mucus was fractionated by the method of differential centrifugation (at 15, 000 g for 1 h, at 105, 000 or 170, 000 g for 3 h) and gel chromatography in columns packed with sepharose 4b. the sediment fractions contained 30--80% of high molecular polysaccharide protein (peptide) mucus components which were toxic for mice and protected 25--77% of rats against experimental p. ae ... | 1979 | 116456 |
| [melanin-forming cultures of pseudomonas aeruginosa]. | 1979 | 116457 | |
| evaluation of the moving intermediate zone concept for determing susceptibility of pseudomonads to gentamicin by the standardized disk agar-diffusion test. | the usefulness of the moving intermediate zone concept for improving the performance of the standardized disk agar-diffusion test in measuring susceptibility of the fluorescent group of pseudomonads to gentamicin was studied. for this purpose, a 3-mm moving intermediate zone lying --3 mm and --6 mm below that measured for the quality control microorganism pseudomonas aeruginosa, atcc 27853, and a wider moving intermediate zone lying --2 mm and --7 mm below that for the quality control microorgan ... | 1979 | 116543 |
| [pseudomonas aeruginosa infections in an oncological center (author's transl)]. | distribution of pseudomonas aeruginosa was observed, both in cancerous hospitalized and ambulatorial patients, through uroculture or by screening at the time of admission in hospital. the data have been statistically evaluated according to the specific sectorial activities. the chemiosensitivity of isolated strains was evaluated for eight antibiotics, selected among the most active ones. minimal inhibent concentration (mic) and minimal bactericidal concentration (mbc) were also evaluated for gen ... | 1978 | 116583 |
| competition of beta-lactam antibiotics for the penicillin-binding proteins of pseudomonas aeruginosa, enterobacter cloacae, klebsiella aerogenes, proteus rettgeri, and escherichia coli: comparison with antibacterial activity and effects upon bacterial morphology. | the competition of a number of beta-lactam morphogenic probes for the penicillin-binding proteins (pbps) of pseudomonas aeruginosa, enterobacter cloacae, klebsiella aerogenes, proteus rettgeri, and escherichia coli has been studied. the results indicate that the various gram-negative bacteria have similar, but not identical, pbp patterns and that the individual proteins probably perform similar morphogenic functions as in e. coli k-12. comparison of the 50% binding concentrations of the compound ... | 1979 | 116592 |
| lytic effect of di- or tricarboxylic acids plus sodium dodecyl sulfate against pseudomonas aeruginosa. | pseudomonas aeruginosa was rapidly lysed and killed when treated with several multiple-carboxylic acids at 100 micrograms/ml, followed by exposure to sodium dodecyl sulfate at 5 mg/ml. | 1979 | 116594 |
| biochemical and genetic characterization of three hamster cell mutants resistant to diphtheria toxin. | we describe here three different hamster cell mutants which are resistant to diphtheria toxin and which provide models for investigating some of the functions required by the toxin inactivates elongation factor 2 (ef-2). cell-free extracts from mutants dtx(r)-3 was codominant. the evidence suggests that the codominant phenotype is the result of a mutation in a gene coding for ef-2. the recessive phenotype might arise by alteration of an enzyme which modifies the structure of ef-2 so that it beco ... | 1979 | 117016 |
| bactericidal antibody response to pseudomonas aeruginosa by adults with urinary tract infections. | in this investigation we found that adults with upper urinary tract infections caused by pseudomonas aeruginosa produced serum antibodies with bactericidal activity against the bacterium. seventeen of 20 infected adults showed bactericidal activity with a titer range of 1:10 to 1:10,000. | 1979 | 117024 |
| [effect of high hydrostatic pressure on the formation of keto- and amino acids by a barotolerant strain of pseudomonas aeruginosa]. | 1979 | 117033 | |
| microbial contamination of topical medicaments used in the treatment and prevention of pressure sores. | topical medicaments used in the treatment and prevention of pressure sores in patients in three hospitals were examined for pseudomonas aeruginosa and staphylococcus aureus contamination. contamination rates were found to vary between hospitals and were affected by differences in the packaging of the product and in the method of application used by the nursing staff. | 1979 | 117050 |
| light-mediated changes in pigmentation of pseudomonas aeruginosa cultures. | cultures of pseudomonas aeruginosa pao grown under uninterrupted broad-spectrum light showed different pigmentation from dark-grown cultures. whereas dark-grown bacteria produced pigments which resulted in blue-purple coloured agar, light-grown organisms produced red coloured plates. extraction and quantification of pigments showed that both dark- and light-grown cultures produced similar concentrations of pyorubrin (red) and pyoverdin (yellow). in contrast, the concentration of pyocyanin (blue) ... | 1979 | 117077 |
| bactericidal activity of the tail of pseudomonas aeruginosa bacteriophage ps17. | the tail of bacteriophage ps17 of pseudomonas aeruginosa was shown to be bactericidal, and its properties were compared with those of pyocin r1. temperature-sensitive mutants were isolated from ps17, and the products at nonpermissive temperature were morphologically characterized. bactericidal substances were found in the lysates of such mutants that were defective in the head formation but not in the tail formation. phage tails were purified from the lysate of one such mutant, and its chemical ... | 1979 | 117121 |
| induction of bacteriolytic enzyme from pyocinogenic pseudomonas aeruginosa and its enzymatic properties. | mitomycin c induced a pyocinogenic pseudomonas aeruginosa p15 to produce a bacteriolytic enzyme, pr1-lysozyme, together with pyocin r1. no significant accumulation of the enzyme was observed inside the induced cells. the enzyme was partially purified by acrinol treatment and amberlie cg-50 column chromatography. the mode of action of the enzyme on the host bacterial cells as well as on micrococcus lysodeikticus cells or peptidoglycan isolated from salmonella typhimurium, was compared with that o ... | 1978 | 117282 |
| [pyocin typing of pseudomonas aeruginosa strains isolated from patients with urologic diseases]. | 1979 | 117286 | |
| [products of bacterial destruction of sodium dodecyl sulphate]. | the products of na dodecyl sulphate destruction by the three bacterial cultures--pseudomonas aeruginosa, flavobacterium devorans, achromobacter guttatus--were examined. the cultures were shown to decompose na dodecyl sulphate in a similar way. the primary mechanism of destruction was found to be hydrolysis of the sulpho-ester bond in the molecule, leading to the separation of sulphate-ion and formation of dodecanol. products of bacterial destruction of alkyl sulphates did not show foam forming c ... | 1979 | 117448 |
| purification of a cytotoxic protein from pseudomonas aeruginosa. | 1979 | 117571 | |
| autoantibodies in serum and sputum from patients with cystic fibrosis. | sera from 89 patients with cystic fibrosis (cf) and 88 control persons were examined for the occurrence of rheumatoid factors (rf) of the igg, iga and igm classes by an indirect immunofluorescence method and by the latex fixation slide test. the prevalence of rf-igg was significantly higher (88%) (p less than 0.0005) among the cf patients than among the control persons (7%), while no difference was found between the two groups with regard to rf of the iga or igm classes. fifty-five of the cf pat ... | 1979 | 117671 |
| [effect of edta on certain biological properties of pseudomonas aeruginosa]. | 1979 | 117679 | |
| [microbiological approach to urinary infections with pseudomonas aeruginosa]. | 1979 | 117691 | |
| [comparison of the sensitivity of pseudomonas aeruginosa cultures that synthesize melanin and other pigments to 12 antibiotics and 5-nitro-8-quinolinol]. | sensitivity of 80 pseudomonas aeruginosa cultures forming melanin and 80 cultures synthesizing other pigments to amikacin, gentamicin, kanamycin, carbenicillin, levomycetin, monomycin, 5-nok, polymyxin m, rifampicin, streptomycin, tetracycline, tobramycin and erythromycin was determined. it was found that the cultures of ps. aeruginosa synthesizing melanin were less resistant to most of the antibiotics that the other representatives of this species. | 1979 | 117745 |
| effect of divalent cation concentrations on the antibiotic susceptibilities of nonfermenters other than pseudomonas aeruginosa. | the effects of supplementing mueller-hinton broth with calcium and magnesium on the minimal inhibitory concentrations (mics) of eight aminoglycosides, colistin, tetracycline, and carbenicillin for 11 nonfermenters other than pseudomonas aeruginosa were studied and compared with the effects for escherichia coli and p. aeruginosa. mics were simultaneously performed in unsupplemented mueller-hinton broth and mueller-hinton broth supplemented to contain 5 mg of calcium and 2.5 mg of magnesium per dl ... | 1979 | 117746 |
| effect of dyes on the quantitative recovery of yersinia enterocolitica. | a total of 69 dyes were incorporated separately at different concentrations into an agar medium for evaluation of their effects on the quantitative recovery of five serotypes of yersinia enterocolitica. one strain of pseudomonas aeruginosa and one strain of bacillus cereus were included for comparative purposes. certain dyes were evaluated further for their selective properties with five additional serotypes of y. enterocolitica, three strains of p. aeruginosa, and two of engerobacter spp. metan ... | 1979 | 117750 |
| immunogenic properties of slime produced by pseudomonas aeruginosa. | the slime of 8 various pseudomonas aeruginosa strains was purified and used to immunize rabbits. all studied slime-extracts were strongly immunogenic for animals. the level of antibodies against slime was determined by the passive hemagglutination test. immune hemagglutinins were present mainly in the igm fraction of antisera. | 1979 | 117786 |