| passive immunization against pseudomonas with a ribosomal vaccine-induced immune serum and immunoglobulin fractions. | passive protection of mice against pseudomonas aeruginosa using specific antisera and immunoglobulin fractions induced by immunizing rabbits with a ribosomal vaccine is reported. the results demonstrated that protection by the ribosomal vaccine against challenge with live organisms can be serum mediated. previous work has shown that the vaccine can be separated into two components on the basis of molecular weight and that both higher (peak a)- and lower (peak b)-molecular-weight fractions were c ... | 1979 | 106013 |
| [experience with epidemiologic studies of pyocyaneus infections. i. feasibility of using serotypes and antibiotic resistance as a epidemiologic label for clinical strains]. | the authors carried out serological typing of 98 pseudomonas aeruginosa strains, isolated from patients of burn department of the sklifosovsky first aid institute in january-july, 1974, and of 215 strains obtained from other sources; their sensitivity to 13 antibiotics was determined. pseudomonas aeruginosa cultures isolated from the patients were typed with o-sera of 10 serological types. the presence of several hospital strains of pseudomonas aeruginosa was found by means of serological typing ... | 1978 | 105523 |
| [isolation and general characteristics of a group of pseudomonas aeruginosa temperate phages]. | eighteen temperate phages were isolated from 38 natural strains of pseudomonas aeruginosa. the phages belong to five heteroimmune groups according to the ability of lysogenic variants of the strains pao1 and pat2 to exclude the growth of the superinfecting phage. phages of the ii and v immunity groups are serologically similar; the remaining phages are not inactivated by antisera against phages b26 (ii group) and k1338 (v group). prophages of the ii, iv, and v groups are induced by uv for vegeta ... | 1979 | 106221 |
| biosynthesis of peptidoglycan in pseudomonas aeruginosa. 1. the incorporation of peptidoglycan into the cell wall. | ether-treated cells of pseudomonas aeruginosa catalyze the formation of crosslinked peptidoglycan from the two nucleotide precursors uridinediphospho-n-acetylglucosamine and uridinediphospho-n-acetylmuramyl-l-alanyl-d-gamma-glutamyl-meso-diaminopimelyl-d-alanyl-d-alanine. the main enzymatic reactions of biosynthesis were similar to those found in escherichia coli. part of the reaction products were soluble in 4% sodium dodecylsulfate whereas the other part was covalently bound to the preexisting ... | 1979 | 107029 |
| [use of the indirect hemagglutination reaction for detecting antibodies to pseudomonas pyocyanea in acute suppurative destructive pneumonia]. | no less than 4-fold increase in the antibody titres to pseudomonas aeruginosa during the infectious process served as laboratory confirmation of its participation in the infectious process. in 49 of 91 patients hemagglutining titre exceeded the diagnostic one (1:640). in 9 patients (chiefly in infants) hemagglutinin titre remained low, but there was a rise of antibody level to pseudomonas aeruginosa during the disease. high hemagglutinin titres were noted in 12 patients on admission to the clini ... | 1978 | 106610 |
| [antibiotic resistance study of clinical strains of pseudomonas aeruginosa]. | the study of 40 clinical strains of ps. aeruginosa isolated from the wound surfaces of the patients showed that all the isolates were resistant to one or several antibiotics. the number of the strains resistant to 5, 4, 3, 2 or 1 drug was 5, 22.5, 25. 30 or 17.5 per cent respectively. fifteen strains carried resistance plasmids capable of conjugative transfer. eleven out of 21 plasmids controlled resistance to chloramphenicol, 7 plasmids controlled resistance to streptomycin and sulfanylamides, ... | 1979 | 106769 |
| biosynthesis of peptidoglycan in pseudomonas aeruginosa. 2. mode of action of beta-lactam antibiotics. | the intrinsic effect of various beta-lactam antibiotics on the biosynthesis of peptidoglycan of pseudomonas aeruginosa x-48 was investigated. most of the cephalosporins and penicillins tested already at 0.5 microgram/ml strongly inhibited (a) the incorporation of nascent peptidoglycan into the detergent-insoluble fraction (greater than 75%), (b) the formation of peptide crosslinkages (greater than 60%) and (c) the activity of the dd-carboxypeptidase and partially that of the transpeptidase (appr ... | 1979 | 107030 |
| therapeutic experience with netilmicin. | netilmicin, a new aminoglycoside antibiotic, was used to treat 19 patients with urinary tract infection and 5 with systemic infection. the causal organisms were escherichia coli (in 2), klebsiella pneumoniae (in 4), serratia marcescens (in 12) and pseudomonas aeruginosa (in 7); 1 patient was infected with two of these organisms. all the isolates of causal organisms except one of serratia were initially sensitive to netilmicin but many were resistant to other aminoglycosides. sixteen of the urina ... | 1978 | 105797 |
| [morphogenesis of pseudomonas aeruginosa colonies]. | | 1978 | 105746 |
| serotypes and antibiotic susceptibilities of pseudomonas aeruginosa isolates from single sputa of cystic fibrosis patients. | a phenotypic characterization of pseudomonas aeruginosa from single sputum samples of 21 typical cystic fibrosis patients indicated a high frequency of heterogeneity among isolates on the basis of differences in antibiotic resistance, colony morphology, pigmentation, and serotype. two or more isolates with different but stable susceptibilities to carbenicillin, gentamycin, streptomycin, tetracycline, chloramphenicol, and sulfamethoxazole plus trimethoprim were detected in 38% of the sputa. diffe ... | 1979 | 107186 |
| microscopic characterization of rabbit lung damage produced by pseudomonas aeruginosa proteases. | the intratracheal administration of highly purified pseudomonas aeruginosa proteases (ca. 10 to 100 microgram) elicited extensive, grossly observable rabbit lung damage by 3 h postinjection. light and electron microscopic characterization of the lesions revealed: (i) progressive injury and necrosis of type i epithelial cells and capillary endothelial cells from 3 h to 1 day postinjection, and progressively increasing accumulations of erythrocytes, plasma proteins, fibrin, and released type ii e ... | 1979 | 106001 |
| [detection of protease, elastase production by pseudomonas aeruginosa and protease, elastase production of gram-negative bacteria (author's transl)]. | | 1979 | 108437 |
| rapid identification of nonfermentative gram-negative rods by the corning n/f system. | a total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the n/f system and to determine whether the observed results included sufficient key reactions for rapid identification. the system included an oxidase test, two screening tubes providing 5 test reactions designed primarily to identify pigment-producing strains of pseudomonas aeruginosa, and a plate providing 12 test reactions designed to identify other nonfermenters. ... | 1979 | 107190 |
| some biological and immunological properties of extracellular slime from pseudomonas aeruginosa. | extracellular slime from 8 different pseudomonas aeruginosa strains was extracted and purified. all slime-preparations exhibited immunogenic properties in rabbits vaccinated with detoxified or not detoxified slime. the antisera of both groups of immunized animals possessed strong hemagglutination activity against homologous slime. immune hemagglutinins were present in the igm and igg fractions of serum globulins. the high value of these antibodies was found in rabbit's sera short after injection ... | 1978 | 107919 |
| l-forms of pseudomonas aeruginosa. 3. the serological cross-reactions among stable l-forms of pseudomonas aeruginosa, l-form of streptococcus pyogenes and mycoplasmas. | the serological cross-reactions among stable l-forms of pseudomonas aeruginosa, or of streptococcus pyogenes and mycoplasmas were investigated in passive hemagglutination (pha) and agar gel diffusion tests. (1) pha active fraction which reacts most strongly with homologous serum was obtained from the sodium dodecyl sulfate soluble fraction of l-form membrane after disruption by braun cell homogenizer. (2) antiserum prepared against l-forms in rabbits showed high-level stimulation of pha antibodi ... | 1978 | 108440 |
| production of exotoxin, protease and elastase of pseudomonas aeruginosa strains isolated from patients' and environmental specimens. | | 1978 | 108441 |
| rapid diagnosis of pseudomonas aeruginosa urinary tract infections by radioimmunoassay. | a solid-phase radioimmunoassay designed to detect serotype 6 pseudomonas aeruginosa antigens was evaluated for its ability to rapidly diagnose urinary tract infections. twelve p. aeruginosa serotypes were easily differentiated in the assay from eight other gram-negative bacterial species. during log-phase growth, the assay detected antigens in culture when approximately 10(6) or more serotype 6 p. aeruginosa organisms were present. both cell-associated and solubilized antigens were detected. the ... | 1979 | 107191 |
| [cyanide-resistant respiration of pseudomonas aeruginosa bacteria]. | the transition of the bacterial culture into the stationary growth phase is accompanied by an appearance of cyanide-resistant respiration. chloramphenicol inhibits the development of cyanide-resistant respiration. the cyanide-resistant oxidase is localized in the bacterial membrane. its appearance is not due to the quantitative and qualitative changes of flavins, non-heme iron, ubiquinone and cytochromes of the b and c types, but is accompanied by an increase in the copper content of the membran ... | 1979 | 107978 |
| [the influence of anti-oep-igg and -igm upon mouse spleen macrophage. phagocytic activity against pseudomonas aeruginosa and oep-coated latex granules (author's transl)]. | | 1978 | 107248 |
| the role of lactoferrin in the bactericidal function of polymorphonuclear leucocytes. | rabbit polymorphonuclear leucocytes contain the iron-binding protein lactoferrin, and can rapidly phagocytose and destroy pseudomonas aeruginosa. the lactoferrin normally has a large unsaturated iron-binding capacity. if the cells are exposed to a ferritin-antibody complex, large amounts of this are phagocytosed and appear in the cytoplasmic granules and phagosomes. this leads to saturation of the cellular iron-binding protein with fe. in these circumstances, the bactericidal power of the cells ... | 1979 | 108208 |
| pseudomonas aeruginosa bacteremia: relationship of bacterial enzyme production and pyocine types with clinical prognosis in 100 patients. | strains of pseudomonas aeruginosa were studied from 100 patients with bacteremia. in vitro quantitation of extracellular enzymes (lecithinase, protease, and elastase) and pyocine typing of these isolates were performed. no significant difference was found in the quantity of the enzymes produced or in the pyocine types by isolates obtained from patients dying from bacteremia or surviving this serious infection. quantitation of the extracellular enzymes and pyocine types of blood isolates were con ... | 1979 | 107256 |
| defective cellular immunity to gram-negative bacteria in cystic fibrosis patients. | in vitro lymphocyte responses to pseudomonas aeruginosa have been found to be impaired in cystic fibrosis patients with advanced clinical disease. the responses to klebsiella pneumoniae, serratia marcescens, and proteus mirabilis were studied in a similar group of cystic fibrosis patients and normal individuals. cystic fibrosis patients found to be unresponsive to pseudomonas were also unresponsive to klebsiella, serratia, and proteus. responsiveness to staphylococcus aureus was not impaired in ... | 1979 | 106011 |
| comparative antibacterial activity of azlocillin, mezlocillin, carbenicillin and ticarcillin and relative stability to beta-lactamases of pseudomonas aeruginosa and klebsiella aerogenes. | the antibacterial activities of two ureidopenicillins, azlocillin and mezlocillin, were compared with those of the alpha-carboxypenicillins, carbenicillin and ticarcillin, against a large number of gram-positive and gram-negative bacteria. all four penicillins were active against a wide range of bacteria including pseudomonas aeruginosa, but there were differences in the antibacterial spectra and in the antibacterial effects demonstrated by the two classes of penicillins. in particular, the mini ... | 1979 | 108220 |
| the instability of mucoid pseudomonas aeruginosa: fluctuation test and improved stability of the mucoid form in shaken culture. | | 1979 | 107270 |
| aminoglycoside antibiotics. 2. n,n-dialkylkanamycins. | n6',n3''-dialkyl derivatives of kanamycins a and b were prepared regiospecifically from the parent antibiotics. although the dimethyl and diethyl derivatives of kanamycin a were inactive in standard antibacterial assays, the dimethyl derivative of kanamycin b showed moderate activity, especially against various strains of pseudomonas aeruginosa. a method for the selective dimethylation of the 3''-amino group of kanamycin a also was developed. | 1979 | 107311 |
| [investigations on the aminoglycoside inactivating enzyme aac-(6') iv (author's transl)]. | in serratia marcescens and pseudomonas aeruginosa from clinical specimen acetyltransferase aac-(6') iv leads to varying phaenotypically expressed resistance to aminoglycoside antibiotics. the substrate profile and the rate of inactivation of the enzymes investigated are almost identical with each other and with the enzyme of p. aeruginosa gn 315. the enzymes cannot be associated with r factors by the standard methods for conjugation, transformation, density gradient centrifugation and agarose g ... | 1979 | 108221 |
| partial purification of heat-labile hemolysin from pseudomonas aeruginosa. | | 1978 | 107353 |
| [outbreaks and sporadic cases of enteritis due to pseudomonas aeruginosa in premature babies and infants (author's transl)]. | in 1960 there were two outbreaks of mostly severe diarrhoea due to pseudomonas aeruginosa in the special nursery for premature infants and sick newborns of the universitäts-kinderklinik münster/westf. resp. kinderklinik offenbach/main. in both cases a single strain of ps. aeruginosa was isolated from patients with diarrhoea and symptom-free children. the clinical picture ranges from mild diarrhoea to severe enteritis with septicemia. the source of the strain found in the first outbreak was not i ... | 1979 | 108223 |
| [studies of the incidence of healthy carriers of "pathogenic strains" or potentially pathogenic strains in the hospital environment]. | an investigation has been carried out on healthy carriers of "positive staphylococcus coagulase" and "occasional pathogenic" strains among kitchen, canteen and dispensary staff in the ivth geriatric division and first aid clinic of the bologna "m. malpighi" hospital. all stains of "positive staphylococcus coagulase" and "occasional pathogens" considered (pseudomonas aeruginosa and citrobacter) were put through antibiotic and chemotherapeutic sensitivity tests. the results are reported in detail. | 1979 | 107491 |
| incidence of transferable drug resistance in pseudomonas aeruginosa: transfer of carbenicillin resistance to escherichia coli k12 (f-)--a preliminary report. | | 1979 | 107118 |
| [pubic osteitis caused by pseudomonas aeruginosa]. | | 1978 | 107573 |
| [pseudomonas aeruginosa in swimming pool waters (author's transl)]. | during the year 1977 we examined 2226 samples of swimming pool waters for colony counts, escherichia coli and coliforms according to the german standards for drinking water. in addition, pseudomonas aeruginosa was isolated out from 180 of those samples. this microorganism was found in 4.7% of samples from public municipal swimming pools, in 4.1% from swimming pools in schools, in 13.1% from swimming pools in hotels, boarding-houses, hydropathic establishments, sanatoria, blocks of flats and priv ... | 1978 | 107683 |
| influence of genetic factors on natural resistance of mice to pseudomonas aeruginosa. | in order to determine whether genetic background influences natural resistance to pseudomonas aeruginosa, a series of 16 different strains of inbred mice were challenged intraperitoneally with p. aeruginosa. significantly greater natural resistance to infection was found in mice of c3h background genome than in mice of a background genome. this phenomenon was documented for two different strains of p. aeruginosa but could not be demonstrated in control studies in which mice were intraperitoneall ... | 1979 | 108343 |
| minimal antibiotic concentrations of aminoglycosides and beta-lactam antibiotics for some gram-negative bacilli and gram-positive cocci. | the minimal antibiotic concentration (mac) is the lowest concentration of an antibacterial agent that produces a decrease of 1 log in the number of organisms/ml as compared with a control culture in drug-free medium. various gram-negative bacilli and gram-positive cocci were grown in the presence of amikacin, gentamicin, tobramycin, ampicillin, amoxicillin, oxacillin, carbenicillin, ticarcillin, and cefamandole at concentrations varying from eight times the minimal inhibitory concentration (mic) ... | 1979 | 108345 |
| [fundamental studies and clinical evaluation of pc-904 in urinary tract infection (author's transl)]. | measurement of mics of pc-904, cbpc and gm against 135 strains of pseudomonas aeruginosa isolated from patients with urinary tract infections was performed. susceptibility to pc-904 of pseudomonas aeruginosa was found to be 3 approximately 7 times more sensitive than that to cbpc and as same as that to gm with inoculum size of 10(8)/ml and 10(6)/ml. serum level of pc-904 reached a peak of 38.5 microgram/ml 15 minutes after 0.5 g intravenous administration. urinary recovery rate unit 6 hours afte ... | 1979 | 108428 |
| ige antibody production to exoenzymes and common antigen (oep) of pseudomonas aeruginosa in mice. | ige antibody production to exoenzymes and common antigen (oep) of p. aeruginosa was studied in mice that were injected with the antigens incorporated into water-in-oil-in-water (w/o/w) emulsion or al(oh)3 gel. oep and protease toxoid (pt) elicited ige antibody response but elastase toxoid (et) did not. capacity of the oep to produce ige antibody was reduced remarkably by it's protease treatment, which suggested that the capacity lies in the protein portion of oep. the w/o/w emulsion was less eff ... | 1978 | 108438 |
| passive hemagglutination reaction using formalinized sheep erythrocytes treated with tannic acid and coated with exotoxin of pseudomonas aeruginosa. | passive hemagglutination (pha) tests using fixed sheep erythrocytes coated with exotoxin of pseudomonas aeruginosa were devised for estimating antibodies of the exotoxin. no serological cross-reactivity among exotoxin oep, protease and elastase of p. aeruginosa was foud by the pha tests. the exotoxin pha reaction was more sensitive than the neutralizing reaction of skin necrotizing activity of exotoxin by guinea pigs. thirteen out of 40 sera of patients with p. aeruginosa infections showed pha t ... | 1978 | 108439 |
| [various extra- and intracellular enzymes of pseudomonas aeruginosa strains]. | | 1978 | 108484 |
| transformation of pseudomonas aeruginosa and escherichia coli with resistance plasmid dna: formation of smaller conjugative plasmids from rpi. | sheared dna from rpi, and r plasmid from a strain of pseudomonas aeruginosa, was used to transform other strains of p. aeruginosa and escherichia coli. from transformed cells other plasmids like rpi were isolated. these deletion plasmids were conjugally transferrable and confer resistance mainly against carbenicillin and tetracycline. | 1979 | 108516 |
| [conditions for the manifestation of cyanide-resistant respiration in pseudomonas aeruginosa]. | conditions for manifestation of cyanide-resistant respiration were studied in pseudomonas aeruginosa. transition of the culture from the logarithmic to the stationary phase of growth, once the source of carbon (hexadecane, glucose, citrate, succinate) or nitrogen was exhausted, was accompanied with a decrease in the sensitivity of bacterial respiration to cyanide. as soon as the limiting factor was added, the culture started to grow again and its respiration became more sensitive to the inhibito ... | 1979 | 108524 |
| effect of pseudomonas aeruginosa, isolated from cervices of females with sterility complaints, on sperm motility. | | 1978 | 108212 |
| [comparison of two methods of antibiotic sensitivity test: gel diffusion and automatic method using abac apparatus. ii. study on 399 strains isolated in hospital (author's transl)]. | the authors compared for routine use, the results obtained on 399 strains of gram negative bacilli by two technics of antibiotic sensitivity: the technic of gel diffusion with imprenated discs and an automatic method (abac apparatus). among the antibiotics used as a routine in our laboratory by the diffusion method and the 16 studied with the abac apparatus, 11 are common to both methods and were compared. the concordance of the results was greater than 90% for colistine (94.5%), oxolinic acid ( ... | 1978 | 107836 |
| effect of listeria monocytogenes lipids on the course of infections with some gram-negative bacilli in mice. | the effect of listeria monocytogenes lipids on the course of infections with salmonella enteritidis, yersinia pseudotuberculosis, pseudomonas aeruginosa and klebsiella pneumoniae was studied in mice. the lipids were extracted with chloroform and methanol and used in various doses before and after infection with the bacilli. the lipids significantly increased mouse resistance toward the examined bacteria. the protective effect depended, to a large extent on the applied dose of lipids and its timi ... | 1979 | 109064 |
| evaluation of aeruginocine and serological typing methods in pseudomonas aeruginosa infection. | | 1979 | 109394 |
| membrane-bound d-gluconate dehydrogenase from pseudomonas aeruginosa. purification and structure of cytochrome-binding form. | a membrane-bound d-gluconate dehydrogenase [ec 1.1.99.3] was solubilized from membranes of pseudomonas aeruginosa and purified to a homogeneous state with the aid of detergents. the solubilized enzyme was a monomer in the presence of at least 0.1% triton x-100, having a molecular weight of 138,000 on polyacrylamide gel electrophoresis or 124,000--131,000 on sucrose density gradient centrifugation. in the absence of triton x-100, the enzyme became dimeric, having a molecular weight of 240,000--26 ... | 1979 | 109426 |
| conversion of 2,4,6-trinitrophenol to a mutagen by pseudomonas aeruginosa. | a strain of pseudomonas aeruginosa reduced 2,4,6-trinitrophenol (picric acid) to 2-amino-4,6-dinitrophenol (picramic acid) under anaerobic conditions. mutagenic assays of picric acid and picramic acid were carried out with histidine-requiring strains of salmonella typhimurium. picric acid (10 micrograms per plate) demonstrated mutagenicity (both frame shift and base substitution-gype mutations) only after activation with a rat liver homogenate preparation. picramic acid (1 microgram per plate) i ... | 1979 | 107854 |
| pseudomonas aeruginosa vasculitis and bacteremia following conjunctivitis: a simple model of fatal pseudomonas infection in neutropenia. | during attempts to create a realistic model of fatal bacteremia due to pseudomonas aeruginosa during immunosuppression, it was found that the invasive as well as the disseminated phase of infection could be mimicked by gentle instillation of 10(8) colony-forming units of p. aeruginosa into the intact conjunctival sac of agranulocytic rabbits. within 48 hr animals developed conjunctivits leading to severe necrotizing vasculitis and fatal bacteremia. twelve of 26 strains from patients with p. aeru ... | 1979 | 109545 |
| influence of mucoidy on antibody coating of pseudomonas aeruginosa. | antibody-coated bacteria were found in only two of 34 urine sediments from 19 catheterized patients infected with a single epidemic strain of pseudomonas aeruginosa, whereas 12 of 19 urine sediments from 16 outpatients contained antibody-coated p. aeruginosa. in urine sediments, individual cells and microcolonies of the epidemic strain of p. aeruginosa were enclosed in ruthenium red (polysaccharide)-positive material. this strain was extremely mucoid when grown in a liquid medium for enhancement ... | 1979 | 109548 |
| variation in response of pseudomonas aeruginosa to combinations of gentamicin and carbenicillin. | | 1979 | 109586 |
| mapping of the loci involved in the catabolic oxidation of l-hydroxyproline in pseudomonas aeruginosa pao. | genes specifying the oxidative utilization of hydroxyproline (hyp) in p. aeruginosa pao were located on the chromosome, around 19th minute by conjugation experiments. a map order of his-68-his-07-hyp was assigned. confirmation of this gene order was also demonstrated by transductional mapping studies. all the genes determining the enzymes of hyp dissimiliatory pathway were closely linked. | 1979 | 109741 |
| influence of iron on yields of extracellular products in pseudomonas aeruginosa cultures. | the effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of pseudomonas aeruginosa was examined. all strains showed at least an 85% decrease in toxin a yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), whereas bacterial growth increased approximately twofold. during the course of examining extracellular products produced by p. aeruginosa, we found many strains that produced an ext ... | 1979 | 108250 |
| detection of fp plasmids in hospital isolates of pseudomonas aeruginosa. | a simple method of detection of fp plasmids with chromosome-mobilizing ability in pseudomonas aeruginosa has been developed. | 1979 | 108252 |
| morphologic expression of the interactions of human lymphocytes and pseudomonas aeruginosa as observed by scanning electron microscopy. | when human lymphocytes and pseudomonas aeruginosa were incubated together and then observed under the scanning electron microscope, four distinct morphologic observations were made: (1) filopods extending between lymphocytes and bacteria; (2) globular structures bulging out in many of the filopods; (3) filopods connecting lymphocytes to each other in the presence of bacteria; and (4) bacteria adhering to the lymphocytes in areas where decreased numbers of microvilli were present. | 1979 | 108337 |
| [problems of the immunoprophylaxis of experimental pseudomonas pyocyanea wound sepsis]. | an experimental study showed that a multicomponent pseudomonas aeruginosa preparation, developed by the authors and named "pyoimmunogen", had a pronounced protective effect against the culture of p. aeruginosa, most frequently isolated in surgical clinics. the immunization of rats carried out in accordance with a specially devised scheme allowed to decrease mortality rate in experimental pyocyanic wound sepsis to 6.9%, whereas in the control animals mortality rate reached 91.5%. | 1979 | 110018 |
| kinetic mechanism of the aliphatic amidase from pseudomonas aeruginosa. | the kinetic constants for hydrolysis and transfer (with hydroxylamine as the alternate acceptor) of the aliphatic amidase (acylamide amidohydrolase, ec 3.5.1.4) from pseudomonas aeruginosa were determined for a variety of acetyl and propionyl derivatives. the results obtained were consistent with a ping-pong or substitution mechanism. product inhibition, which was ph dependent, implicated an acyl-enzyme compound as a compulsory intermediate and indicated that ammonia combined additionally with t ... | 1979 | 110350 |
| aminopeptidase i activities in several microorganisms. | aminopeptidase i activity which was found to be localized in the same subcellular fraction and to be similarly heat stable was partially purified by a common procedure from escherichia coli b, escherichia coli k12, enterobacter aerogenes, salmonella typhimurium, serratia marcescens pseudomonas aeruginosa, and proteus vulgaris. the enzyme preparations were shown to contain a single animopeptidase active toward both leucylleucine and methionylalanylserine by mixed-substrate initial-velocity kineti ... | 1978 | 110425 |
| release of rhodanese from pseudomonas aeruginosa by cold shock and its localization within the cell. | whole cells of pseudomonas aeruginosa possess rhodanese activity. the enzyme can be released by rapidly resuspending the cells in cold tris--hcl buffer. approximately 95% of the rhodanese activity is released by cold shock. release of the enzyme can be inhibited either by preincubating the cells with mg2+ or by incorporating mg2+ into the shocking buffer. the effect of mg2+ can be reversed by washing the cells twice with buffer prior to cold shock. while rhodanese can be released from p. aerugin ... | 1979 | 110432 |
| the extraction and analysis of lipopolysaccharides from pseudomonas aeruginosa strain pao, and three rough mutants. | three spontaneously arising rough mutants of pseudomonas aeruginosa have been isolated by selection for resistance to virulent lipopolysaccharide (lps) specific bacteriophages. in addition, the first phages specific for rough mutants of p. aeruginosa were isolated. using these phage and autoagglutination patterns in 4% nacl and acriflavine, these mutants could be clearly distinguished from the wild-type strain and each other. chemical analysis of the lps together with chromatographic resolution ... | 1979 | 110433 |
| pseudomonas aeruginosa infection: pathogenesis and therapy. | | 1979 | 110508 |
| [antibacterial in-vitro activity of pipemidic acid and nalidixic acid (author's transl)]. | the in-vitro activity of nalidixic acid and pipemidic acid was investigated in a combined study in 450 freshly isolated bacterial strains using the agar dilution test. gram-positive cocci were resistant to both substances except for a few pipemidic acid sensitive staphylococci. both substances had good antibacterial activity in the gram-negative spectrum. however, the mic values of pipemidic acid were generally clearly lower than those of nalidixic acid. only pipemidic acid showed activity again ... | 1979 | 110574 |
| inhibition of the aliphatic amidase from pseudomonas aeruginosa by urea and related compounds. | the time-dependent inhibition of amidase from pseudomonas aeruginosa strain ai 3 by urea, hydroxyurea and cyanate displayed saturation kinetics fitting a model for the reaction sequence in which formation of a complex in a reversible step was followed by an irreversible step. altered amidases from mutant strains aiu 1n and ouch 4, selected for their resistance to inhibition of growth by urea and hydroxyurea respectively, had altered kinetic constants for inhibition indicating reduced binding cap ... | 1979 | 110589 |
| 2-deoxy-d-lyxo-hexonic acid from 2-deoxy-d-lyxo-hexose by pseudomonas aeruginosa fermentation. | aerobic fermentation of media or solutions containing 2-deoxy-d-lyxo-hexose and calcium carbonate by bacterial cells capable of oxidizing aldoses to aldonic acids was used to prepare 2-deoxy-d-lyxo-hexonic acid; the acid was isolated in a 62% yield in the form of its 1,4-lactone. | 1979 | 110658 |
| production of exotoxin a by pseudomonas aeruginosa in a chemically defined medium. | a defined medium was developed in which easily measured quantities of exotoxin a (pe) were produced by pseudomonas aeruginosa pa-103. the medium contained three l-amino acids (arginine, aspartic acid, and alanine), basal and trace salts including 14 mm k2hpo4, 14 mm glucose, and 140 mm glycerol. the concentrations of amino acids which yielded most satisfactory results were 6 mm alanine, 13 mm aspartic acid, and 16 mm arginine. the identity of pe in the culture supernatant fluid was demonstrated ... | 1979 | 110687 |
| [comparative study of the antibacterial activity of a number of new antibiotics and their combinations in relation to pseudomonas aeruginosa]. | tobramycin and sisomycin proved to have the highest antibacterial activity against 156 clinical strains of ps. aeruginosa and were 4--8 times more effective than monomycin, kanamycin, neomycin and to a lesser extent gentamicin. the combination of mecillinam and sisomycin had a synergistic effect with respect to 26 out of 50 strains of ps. aeruginosa and the combination of mecillinam and tobramycin had a synergistic effect on 18 strains. an antagonistic effect was observed with the use of the abo ... | 1979 | 109038 |
| effect of type-specific active immunization on the development and progression of experimental pseudomonas aeruginosa endocarditis. | rabbits with intracardiac catheters were immunized with heat-killed pseudomonas aeruginosa or saline and challenged with either 10(9) (high inoculum) or 10(7) (low inoculum) pseudomonas. immunization did not decrease the incidence of endocarditis when compared with controls, but it did significantly prolong survival. the longer survival of immunized rabbits after high-inoculum challenge was not due to prolongation of the course of endocarditis but to type-specific protection from early, overwhel ... | 1979 | 110689 |
| assessment of protease (elastase) as a pseudomonas aeruginosa virulence factor in experimental mouse burn infection. | the data presented indicate that in experimental pseudomonas aeruginosa infection of mice, protease enhances the virulence of the organism. anesthetized cba/lü mice were subjected to a 15-s flame burn and infected with a wild-type protease-producing strain and two of its protease-deficient mutants. the average bacterial cell mean lethal dose (ld50) of 3.8 +/- 0.3 standard deviation (log10) for mice infected with the protease-producing p. aeruginosa was at least 1 log lower than the ld50 of the p ... | 1979 | 110690 |
| protease and elastase of pseudomonas aeruginosa: inactivation of human plasma alpha 1-proteinase inhibitor. | the present study indicates that crystalline elastase of pseudomonas aeruginosa is a very potent inactivator of human plasma alpha 1-proteinase inhibitor, the enzyme (e) inactivated the inhibitor (i) almost completely within 1 h at 25 degrees c at a molar ratio of e/i = 1:100. the crystalline p. aeruginosa protease also inactivated the inhibitor, but 100-fold less. sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the alpha 1-proteinase inhibitor inactivated by the elastas ... | 1979 | 110691 |
| depression of the antibody response in pseudomonas aeruginosa-injected mice. | heat-killed pseudomonas aeruginosa inhibit antibody response in c57bl/6 mice. the depression of this response is dependent on the dose of bacteria injected, on the time interval between microorganism injection and antigen administration, and on the nature of the antigen used. cell transfer experiments provide evidence that suppressor cells are not operative in this model. furthermore, the results show that p. aeruginosa induces a marked dose-dependent proliferation of spleen cells in vivo, and t ... | 1979 | 110694 |
| inhibitory effect of pseudomonas aeruginosa on the phagacytic and killing activities of rabbit polymorphonuclear leukocytes: purification and characterization of an inhibitor of polymorphonuclear leukocyte function. | a clinically isolated strain of polymorphonuclear leukocyte (pmn)-resistant pseudomonas was found to produce an extracellular substance (pmn inhibitor) that inhibits the phagocytic and killing activities of pmn. the pmn inhibitor was purified from culture filtrates by precipitation with (nh4)2so4 and chromatography on phosphocellulose, diethylaminoethyl-cellulose, and sephadex g-100. the preparation was homogenous as judged by disc gel electrophoresis. the purified pmn inhibitor appeared to be a ... | 1979 | 110700 |
| inhibitory effect of pseudomonas aeruginosa on the phagocytic and killing activity of rabbit polymorphonuclear leukocytes: mechanisms of action of a polymorphonuclear leukocyte inhibitor. | the polymorphonuclear leukocyte (pmn) inhibitor isolated from a strain of pseudomonas aeruginosa which is resistant to the phagocytic and killing activities of rabbit pmn inhibited migration of pmn and engulfment of latex particles by pmn. in studies of the bactericidal metabolism of pmn, the pmn inhibitor did not inhibit the intracellular activity and extracellular release of lysosomal enzymes. however, the pmn inhibitor caused a decrease of nitro blue tetrazolium reduction. the pmn inhibitor h ... | 1979 | 110701 |
| augmented osmotic hemolysis of human erythrocytes exposed to the galactosephilic lectin of pseudomonas aeruginosa. | | 1979 | 110727 |
| r plasmids r91 and r91a from pseudomonas aeruginosa share only the gene for carbenicillin resistance. | plasmid r91a of pseudomonas aeruginosa strain 9169 is homologous with rp1. plasmid r91 carried by the same strain is related in the tn1 region but is otherwise unrelated to r91a. | 1979 | 110763 |
| attempts to control clothes-borne infection in a burn unit, 2. clothing routines in clinical use and the epidemiology of cross-colonization. | previous investigations have shown that cross-contamination in a burn unit is mainly clothes-borne. new barrier garments have been designed and tried experimentally. the aim of the present study was to investigate the effects of different clothing routines on cross-contamination. in a long-term study, the rates and routes of colonizations with staphylococcus aureus, streptococcus groups a, b, c, f, and g and pseudomonas aeruginosa were examined. the exogenous colonization rates were, with s. aur ... | 1979 | 109498 |
| initiation of translation with pseudomonas aeruginosa phage pp7 rna: nucleotide sequence of the coat cistron ribosome binding site. | initiation complex formation between pp7 rna and ribosomes of pseudomonas aeruginosa and escherichia coli has been investigated. the pp7 rna fragments protected by both species of ribosome have been isolated, and their sequences have been determined. only one binding sites is available on the intact pp7 rna strand, and this site is recognized by ribosomes of both species. the pp7 rna binding site is approximately 38 nucleotides long. it contains two aug sequences and a purine-rich segment near t ... | 1979 | 109813 |
| dna insertion mutagenesis in a pseudomonas aeruginosa r plasmid. | | 1979 | 109872 |
| pseudomonas resistance to gentamicin. | 66 burned patients admitted to the burn unit from jan. to sept., 1977, were studied. topical therapy employed was mafenide or silver sulfadiazine. the most common pathogens isolated in 288 burn wound cultures were pseudomonas aeruginosa (42%), klebsiella-enterobacter (16%), and staph, aureus (13%). pseudomonas was sensitive to gentamicin in only 36.2% of instances. in 1976, 42% of pseudomonas isolates was sensitive. during the period 1969-1973, 85.7% of the pseudomonas was sensitive to gentamici ... | 1979 | 109913 |
| the role of pityrosporum pachydermatis in otitis externa of dogs: evaluation of a treatment with miconazole. | the bacterial and mycotic flora were assessed in 158 ears of dogs with otitis externa and in 101 ears of healthy control dogs. pityrosporum pachydermatis occurred in 57 per cent of ears with otitis externa and in 17 per cent of clinically healthy ears. staphylococci and pseudomonas aeruginosa were the predominant bacteria in otitic ears, micrococci and bacillus spp were the most frequent isolates from clinically healthy ears. p pachydermatis, ps aeruginosa and candida tropicalis occurred in mono ... | 1979 | 109986 |
| transposition of tn7 in pseudomonas aeruginosa and isolation of alk::tn7 mutations. | conjugal crosses with pseudomonas aeruginosa donors carrying the cam-oct and rp4::tn7 plasmids result in transfer of the tn7 trimethoprim resistance (tp(r)) determinant independently of rp4 markers. all tp(r) exconjugants which lack rp4 markers have cam-oct genes and therefore must have received cam-oct::tn7 plasmids formed by transposition of tn7 from rp4::tn7 to cam-oct. most crosses yield exconjugants carrying mutant cam-oct plasmids which no longer determine either camphor or alkane utilizat ... | 1979 | 110782 |
| standardization of direct susceptibility test for blood cultures. | insufficient data are available to establish the reliability of direct disk diffusion susceptibility tests performed utilizing positive blood culture broth as inoculum. when staphylococcus aureus atcc 25923, escherichia coli atcc 25922, and pseudomonas aeruginosa atcc 27853 were used, 0.03 ml of turbid overnight blood culture broth was found to produce zone diameters closely approximating the size of diameters obtained by a standardized method. results of direct (0.03 ml of inoculum) and standar ... | 1979 | 110825 |
| use of 2-aminoacetophenone production in identification of pseudomonas aeruginosa. | a grapelike odor is often of diagnostic importance in detecting the growth of pseudomonas aeruginosa in culture and in burn wounds. the compound responsible for the odor has been identified as 2-aminoacetophenone by mass spectroscopy. although the grape odor is sometimes difficult to detect in culture media, gas chromatographic, fluorometric, and colorimetric methods can be utilized to assay 2-aminoacetophenone production in a variety of media. its synthesis occurs relatively early in the growth ... | 1979 | 110829 |
| a more sensitive plate assay for detection of protease production by pseudomanas aeruginosa. | a plate assay to measure protease production by pseudomonas aeruginosa is described. this assay is more sensitive and rapid than established methods. | 1979 | 110831 |
| lipopolysaccharide pseudomonas vaccine: efficacy against pulmonary infection with pseudomonas aeruginosa. | pneumonia due to pseudomonas aeruginosa occurs with increased frequency and high mortality in certain populations of patients. the potential of vaccination with a heptavalent lipopolysaccharide pseudomonas vaccine for specific protection of respiratory tissues from infection with pseudomonas was evaluated with a guinea pig model of experimental pseudomonas pneumonia. animals routinely responded to vaccination with a fourfold rise in titer of serum hemagglutinating antibody to pseudomonas. of 25 ... | 1979 | 110888 |
| pseudomonas aeruginosa bacteremia: susceptibility of 100 blood culture isolates to seven antimicrobial agents and its clinical significance. | the susceptibility of 100 blood culture isolates of pseudomonas aeruginosa observed during 4 1/2 years was tested for tobramycin, netilimicin, gentamicin, amikacin, pirbenicillin, ticarcillin and carbenicillin, singly and in combination. for aminoglycosides, the agar mics were twofold to threefold greater than tube dilution mics but for the penicillins they were similar. for aminoglycosides and ticarcillin, the mbcs were twofold greater than the tube dilution mics. the mbcs were not achieved at ... | 1979 | 110892 |
| [treatment of pseudomonas aeruginosa pneumonia with amikacin (author's transl)]. | | 1979 | 110964 |
| suppressor mutation in pseudomonas aeruginosa. | suppressor mutations were identified in pseudomonas aeruginosa, and a comparison was made with escherichia coli suppressor systems. a suppressor-sensitive (sus) derivative of a plasmid, rp4 trp, and several sus mutants of incp1 plasmid-specific phages, were isolated by using e. coli. plasmid rp4 trp (sus) was transferred to p. aeruginosa strains carrying trp markers which did not complement rp4 trp(sus), and trp+ variants were selected. some, but not all such revertants, could propagate prd1 sus ... | 1979 | 110767 |
| biosynthesis of phenazine pigments in mutant and wild-type cultures of pseudomonas aeruginosa. | pigmentation mutants of pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. three classes of mutants that were incapable of pyocyanine production were identified. pigmentation patterns that were found to characterize the various mutant classes implicated precursor-product relationships, and a biochemical scheme covering the terminal reactions of pyocyanine biosynthesis is proposed. amo ... | 1979 | 110770 |
| transduction of pseudomonas aeruginosa with a mutant of bacteriophage e79. | a mutant of the virulent bacteriophage e79 was isolated which mediated generalized transduction in pseudomonas aeruginosa. variable recovery of transductants as a result of phage killing was avoided by the use of recipients carrying the incp-2 plasmid r38, and transduction frequencies of 4 x 10(-6) to 1 x 10(-5) per plaque-forming unit were obtained. linkage studies have indicated that the coinheritance frequencies are less than would be expected from the published molecular weight of e79 deoxyr ... | 1979 | 110777 |
| toxin inhibitors of protein synthesis: production, purification, and assay of pseudomonas aeruginosa toxin a. | | 1979 | 111001 |
| [melanin-producing strains of pseudomonas aeruginosa]. | | 1979 | 111005 |
| [pathogenicity of clinical strains of pseudomonas aeruginosa]. | the study of 208 ps. aeruginosa clinical strains, introduced intraperitoneally into white mice, revealed the statistically significant prevalence of pathogenic cultures (87.5--100%). the pathogenic strains of ps. aeruginosa were found to have statistically significant differences in their cultural and biochemical properties depending on the kind of clinical material: the strains isolated from blood formed mucoid colonies, the zones of hemolysis and thermolabile or thermostable alkaline phosphata ... | 1979 | 111443 |
| precipitating antibodies against escherichia coli, bacteroides fragilis ss. thetaiotaomicron and pseudomonas aeruginosa in serum from normal persons and cystic fibrosis patients, determined by means of crossed immunoelectrophoresis. | | 1979 | 111468 |
| [gentamycin activity in combination with etonium in relation to pseudomonas aeruginosa]. | gentamicin is one of the most effective drugs for treatment of infections caused by ps. aeruginosa. however, isolation of the strains resistant to the antibiotics was not infrequent. it was shown in the experiments with 40 cultures that the activity of gentamicin against ps. aeruginosa increased approximately 20 times when it was used in combination with ethonium, a derivative of bis-quaternary ammonium compounds. | 1979 | 111608 |
| in vitro activity of piperacillin compared with that of carbenicillin, ticarcillin, ampicillin, cephalothin, and cefamandole against pseudomonas aeruginosa and enterobacteriaceae. | piperacillin (t-1220), a semisynthetic derivative of aminobenzylpenicillin, was more active than either carbenicillin or ticarcillin against pseudomonas aeruginosa; over 60% of isolates were inhibited at a concentration of 6.3 mug/ml. piperacillin was bactericidal for 84% of pseudomonas strains at 100 mug/ml, carbenicillin killed 60%, and ticarcillin killed 68% at that concentration. piperacillin was also more active than the other penicillins against isolates of escherichia coli, enterobacter, ... | 1979 | 111609 |
| comparative study of anti-pseudomonas activity of azlocillin, mezlocillin, and ticarcillin. | the anti-pseudomonas activities of azlocillin and mezlocillin were compared with that of ticarcillin. we measured the minimal inhibitory and minimal bactericidal concentrations of the three drugs against 20 different strains of pseudomonas aeruginosa and found significantly lower values for azlocillin than for the other two drugs. we then infused 5 g of each drug into 10 volunteers on three consecutive days and determined the serum levels of the three antibiotics at 1-h intervals from 1 to 6 h a ... | 1979 | 111610 |
| peptidoglycan transpeptidase inhibition in pseudomonas aeruginosa and escherichia coli by penicillins and cephalosporins. | peptidoglycan transpeptidase activity has been studied in cells of escherichia coli 146 and pseudomonas aeruginosa 56 made permeable to exogenous, nucleotide-sugar peptidoglycan precursors by ether treatment. transpeptidase activity was inhibited, in both organisms, by a range of penicillins and cephalosporins, the pseudomonas enzyme being more sensitive to inhibition in each case. conversely, growth of e. coli 146 was more susceptible to these antibiotics than growth of p. aeruginosa 56. furthe ... | 1979 | 111613 |
| comparative in vitro activity of azlocillin, ampicillin, mezlocillin, piperacillin, and ticarcillin, alone and in combination with an aminoglycoside. | the in vitro activities of the newer semisynthetic penicillins azlocillin, mezlocillin, and piperacillin were compared with those of ampicillin and ticarcillin by using 290 clinical laboratory isolates. piperacillin and mezlocillin were the most active against escherichia coli, proteus mirabilis, klebsiella spp., and enterobacter spp. when pseudomonas aeruginosa was tested, piperacillin and azlocillin were more active than either mezlocillin or ticarcillin. streptococcus pneumoniae and haemophil ... | 1979 | 111616 |
| [pyocin typing of 111 strains of pseudomonas aeruginosa isolated in tuscany, using the gillies and govan method]. | pseudomonas aeruginosa strains from tuscany have been typed for pyocine production against govan and gillies' extended set of indicator strains; results have been expressed following a binary code. among 111 strains only 6 could not be typed; the remaining 105 strains (94.6%) presented 59 different patterns, 44 of which were represented by one isolate only. the most commonly encountered pattern (n. 8045, corresponding to govan and gillies' subtype 1e) was shared by 13 strains. isolates from outp ... | 1978 | 111627 |
| [reiterant contaminations by "pseudomonas aeruginosa" on culture cell lines in a hospital virology service (author's transl)]. | the selection of antibiotic resistant pseudomonas aeruginosa strains by an indiscriminate administration of antibiotics has been observed. the consequences induced by ps. aeruginosa on the tissue culture cell lines (both primary and continuos) are harmful, velding a sharp ph drop from a slight initial increase, followed by the destruction of the cell layer. sometimes the cells succeed in growing up for some passages since antibiotics are usually present in the growth medium. tissue culture conta ... | 1978 | 111632 |
| regions of the lipopolysaccharide of pseudomonas aeruginosa essential for antitumor and interferon-inducing activities. | resistance against ascites tumor development and interferon-inducing activity were demonstrated in lipopolysaccharide derived from the protein-lipopolysaccharide complex obtained from an autolysate of pseudomonas aeruginosa. lipid a obtained from the lipopolysaccharide was sufficient to induce interferon in vitro but no antitumor activity was found if lipid a or the polysaccharide derived from lipopolysaccharide was injected into the animal. chemical modification of the polysaccharide portion or ... | 1979 | 111929 |
| genetic mapping of the region c of the bacteriophage g101 (pseudomonas aeruginosa). | morphological mutants of the c type of the bacteriophage g101 (pseudomonas aeruginosa) were isolated after mutagenesis with hydroxylamine. complementation analysis of 27 c mutants showed that the c region is formed by at least two genes. two types of c mutants were obtained. one of them (ci26) behaves analogously to a mutant in the gene controlling the synthesis of the repressor of phage lambda. the second type of the c mutants (cii1, cii18) specifies a gene having probably an auxiliary function ... | 1979 | 112014 |