Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| purification and properties of two deoxyribonucleases of pseudomonas aeruginosa. | a survey of the major deoxyribonucleases in pseudomonas aeruginosa strain pao was undertaken. two activities predominated in brij-58 lysates of this organism. these have been purified from contaminating nuclease activities, and some of their properties have been elucidated. the first was a nuclease that degraded heat-denatured deoxyribonucleic acid (dna) to mono- and dinucleotides. the activity of this enzyme was confined to single-stranded dna, and 100% of the substrate was hydrolyzed to acid-s ... | 1976 | 60331 |
| pseudomonas aeruginosa antibodies in human plasma. | plasma samples from healthy adults were examined for antibodies to the common protective antigen (oep) [1] by passive hemagglutination (ha) reaction [2] with the finding that the majority of them had an antibody titer of 60 or lower. 2-mercaptoethanol (2-me) treatment, however, caused a decrease in ha titer down to 16 or lower in most of the cases, suggesting that the oep antibody resides mostly in igm and slightly in igg. the finding was corroborated by gel filtration on sephadex g-200. it appe ... | 1976 | 60502 |
| [experimental studies on the reaction of the immunological system during chronic otitis media and about the course of this disease (author's transl)]. | during chronic otitis media intact immunoglobulins are split due to the proteolytic activity of extracellular bacterial proteinases into fragments of different molecular weight. the most malignant bacterial proteinases are the proteinases of pseudomonas aeruginosa (pyrocyanea). these proteinases can only be inhibited by alpha-2-macroblobulin of human blood serum. because of its high molecular weight we find this inhibitor only in a very low concentration in the middle ear secretion. the destruct ... | 1976 | 60682 |
| [increase of resistance to pseudomonas aeruginosa infection in mice caused by staphylococcus aureus]. | in our study of opportunistic pathogens, we have some indication that staphylococcus aureus can increase resistance in mice against pseudomonas aeruginosa. intraperitoneal injections of sublethal doses of s. aureus had a protective effect in mice against lethal doses of p. aeruginosa, more so if living and coagulase-positive s. aureus strains were injected. this protective effect was obtained both with laboratory and freshly isolated hospital strains. the interval between these infections can be ... | 1976 | 61060 |
| [use of salts of quaternary ammonium base for creation of selective media for pseudomonas aeruginosa]. | 1976 | 62092 | |
| "5-amino-5"-deoxybutirosin, a semisynthetic analogue of butirosin a: antibacterial activity in vitro and in mice. | aminodeoxybutirosin (ad-btn), the 5''-amino-5''-deoxy derivative of butirosin (btn), was synthesized to improve on the antibacterial activity of btn by preventing bacterial enzymatic phosphorylation at the 5'' position. ad-btn possesses the spectrum characteristic of btn and gentamicin (gtm) and was active at low levels in vitro against a wide variety of gram-negative species including pseudomonas aeruginosa, indole-positive proteus and serratia marcescens; its action was bactericidal against bo ... | 1976 | 62554 |
| role of ionic strength in salt antagonism of aminoglycoside action on escherichia coli and pseudomonas aeruginosa. | studies were designed to determine whether ionic strength (mu) is a significant factor in salt inhibition of aminoglycoside action against escherichia coli and pseudomonas aeruginosa. in both nutrient broth (a low mu medium) and mueller-hinton broth (a relatively high mu medium), protection of e. coli from dihydrostreptomycin or gentamicin action by mgcl2, nacl, or na2so4 was attributed to ionic strength alone. the percentage of protection increased with ionic strength and was independent of the ... | 1976 | 62813 |
| in vitro activity of amikacin and ten other aminoglycoside antibiotics against gentamicin-resistant bacterial strains. | sixty-nine strains of gentamicin-resistant gram-negative bacilli obtained from different geographical sources were tested for susceptibility to 11 aminoglycoside antibiotics. from the results of determinations of minimal inhibitory concentrations, patterns of resistance were established for 45 strains of enterobacteriaceae and 24 strains of pseudomonas aeruginosa. overall, 81% of the strains were sensitive to amikacin and 33% of the strains were sensitive to butirosin, the next most active compo ... | 1976 | 62815 |
| [the effect of ear surgery to the immunologic defense system of the middle ear during chronic otitis media (author's transl)]. | alpha-1-antitrypsin and alpha-2-macroglobuline are very important proteinase inhibitors for the healing process in chronic otitis media, because they can inhibit numerous bacterial and leukocytic proteinases: alpha-2-macroglobuline can block the malignant proteinases of pseudomonas aeruginosa, staphylococcus aureus and proteus vulgaris. in 58 cases in the middle ear secretion was examined and the level of alpha-1-antitrypsin and alpha-2-macroglobulin investigated pre- and postoperatively. by the ... | 1976 | 63098 |
| electron-microscope studies of the effect of subinhibitory concentrations in phenylethanol and polysorbate 80 on pseudomonas aeruginosa 'sensitive' and 'resilient' to benzalkonium chloride. | 1976 | 63547 | |
| [specificity of antimicrobial immunity in experimental infection with strains of pseudomonas aeruginosa]. | 1976 | 65079 | |
| response of cultured mammalian cells to the exotoxins of pseudomonas aeruginosa and corynebacterium diphtheriae: differential cytotoxicity. | the sensitivities of 21 mammalian cell lines to the exotoxins of pseudomonas aeruginosa and corynebacterium diphtheriae were measured. each line exhibited 1-4 log differences in sensitivities to the two toxins. no species-specific sensitivities were noted for pseudomonas exotoxin while diphtheria exotoxin was most potent in cells of monkey origin, followed by human and hamster cells. rat- and mouse-derived cell lines were very insensitive to diphtheria exotoxin. the rates of cellular intoxicatio ... | 1977 | 65205 |
| amikacin resistance developing in patients with pseudomonas aeruginosa bronchopneumonia. | 1977 | 65626 | |
| pseudomonas aeruginosa infection in cystic fibrosis. relative prevalence of antibodies in serum against cathodic and anodic migrating p. aeruginosa antigens determined by immunoelectrophoretic methods. | sera from 119 patients with cystic fibrosis were examined for precipitating antibodies against anodic migrating p. aeruginosa antigens by means of crossed immunoelectrophoresis. the presence of precipitins against cathodic migrating p. aeruginosa antigens was investigated by means of classical immunoelectrophoresis. thirty-six per cent of the sera contained precipitins against both anodic and cathodic antigens, 20% contained only precipitins against anodic antigens, none contained only precipiti ... | 1977 | 67746 |
| [giant urinary bladder calculi in patients with pseudomonas aeruginosa and proteus vulgaris infections following prostatectomy for prostatic adenoma]. | 1977 | 68625 | |
| incidence of exotoxin production by pseudomonas species. | pseudomonas aeruginosa exotoxin a has been shown to catalyze the transfer of the adenosine 5'-diphosphate (adp)-ribose moiety of nicotinamide adenine dinucleotide onto elongation factor 2, resulting in the inhibition of mammalian protein synthesis. the enzymatic activity (adp-ribosyl [adpr]-transferase) is thought to account for the toxicity of exotoxin a. the distribution of the expression of exotoxin a within pseudomonas species was examined. laboratory strains as well as clinical isolates of ... | 1977 | 68931 |
| a polyvalent human gamma-globulin immune to pseudomonas aeruginosa: passive protection of mice against lethal infection. | as a means to development of guidelines for therapeutic application to human disease, preparations of human polyvalent gamma-globulin immune to pseudomonas aeruginosa (pg) were studied in acute infections in mice. pg was highly effective in controlling lethal infections induced in mice by the major immunotypes of p. aeruginosa; greater than or equal to 10 microgram of of gamma-globulin per mouse protected against challenge with less than or equal to 10(6) 50% lethal doses of p. aeruginosa. pg wa ... | 1977 | 70492 |
| resistance of pseudomonas aeruginosa strains isolated from drugs and hospital material. | 1977 | 70978 | |
| serological typing of pseudomonas aeruginosa--comparison of various antigenic schema. | serotyping sera for both the agglutination (using imsut sera) and slide agglutination (using tibs sera) were succesfully prepared using homma's serotype strains. they were proved to be type specific and distinct. the two typing sera (imsut and tibs) were used to determine the correspondence of major o-antigens among the different kinds of serotype schema which are used at present throughout the world. the serotype sera of lanyi, liu and meitert were also used for the same purpose. the relationsh ... | 1977 | 72174 |
| antigenic relationship between the common antigen (oep) of pseudomonas aeruginosa and vibrio cholerae. | antibodies were found by the oep-passive hemagglutination test to cross-react with the common antigen (oep) of pseudomonas aeruginosa in sera of rabbits immunized with two serotype (inaba and ogawa) strains of vibrio cholerae. the titer in the oep-passive hemagglutination reaction rose later than did the agglutinin titer and reached a peak of 640 to 1,280. the titers of oep antibody formation in rabbits immunized with v. cholerae were almost the same as that of p. aeruginosa. the common antigen ... | 1978 | 75846 |
| immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients. | by the use of specific antibody to human chorionic gonadotropin (cg) as well as to its beta-subunit, and the application of the indirect fluorescein-labeled and peroxidase-labeled antibody techniques, we have demonstrated the presence of a membrane (wall)-associated cg-similar immunoreactive protein in 15 strains of bacteria isolated from tissues of patients bearing malignant neoplasms. these microorganisms were classified as s. epidermidis, (12) e. coli (2), and a single strain of p. maltophili ... | 1978 | 76504 |
| noma neonatorum: its aetiopathogenesis. | noma neonatorum, a gangrenous process affecting the nose, lips, mouth, anal region, and occasionally the scrotum and eyelids, affects neonates, especially low-birth-weight and premature ill babies, and is usually fatal 1-3 days after onset. in 35 cases of noma neonatorum pseudomonas aeruginosa was isolated from blood-culture (86.3%), gangrenous areas (96.0%), rectal swabs (58.3%), and cerebrospinal fluid (60.0%). blood-vessels in the deep cutis or subcutis were affected and the gangrenous proces ... | 1978 | 79084 |
| the primary structure of the coat protein of pseudomonas aeruginosa rna-bacteriophage pp7 [proceedings]. | 1977 | 79389 | |
| [use of agar medium with acetamide as a medium for isolation of pseudomonas aeruginosa]. | 1978 | 79676 | |
| [pigment formation in cultures of pseudomonas aeruginosa isolated from different pathological material]. | 1978 | 79690 | |
| low mortality in burned patients in a pseudomonas vaccine trial. | in a controlled clinical trial of a polyvalent pseudomonas vaccine in burned patients infected with pseudomonas aeruginosa all 18 vaccinated patients survived, whereas 8 of 20 unvaccinated patients died. | 1978 | 79764 |
| [sensitivity of microorganisms isolated from swine to aqueous and proteinic solutions of gentamycin]. | employing a microbiologic method (in vitro) the activity was investigated of gentamicin dso pharmachim, dissolved in water, in normal swine gamma-globulin, and in normal swine polyglobulin with regard to swine strains of escherichia coli, salmonella choleraesis bordetella bronchiseptica, and pseudomonas aeruginosa as well as mycoplasms. a strong sensitivity was found to exsist in all tested strains (except for the mycoplasms) to gentamycin both in a water and in a protein solution. the in vivo t ... | 1978 | 80055 |
| mutational biosynthesis of butirosin analogs. ii. 3', 4'-dideoxy-6'-n-methylbutirosins, new semisynthetic aminoglycosides. | a pair of new butirosin analogs was isolated from the fermentation broth obtained by cultivating a neamine-negative mutant of the butirosin-producing organism bacillus circulans in the medium supplemented with 6'-n-methylgentamine c1a. these antibiotics were characterized and elucidated as 3', 4'-dideoxy-6'-n-methylbutirosins a and b (dmb-a & dmb-b), by chemical and spectroscopic studies. dmb-a and dmb-b exhibited broad-spectrum antibacterial activities with in vitro potency similar to or slight ... | 1978 | 81823 |
| mutational biosynthesis of butirosin analogs. iii. 6'-n-methylbutirosins and 3', 4'-dideoxy-6'-c-methylbutirosins, new semisynthetic aminoglycosides. | two pairs of butirosin analogs were isolated from the fermentation broths obtained by cultivating a neamine-negative mutant of the butirosin-producing organism bacillus circulans in the medium supplemented with 6'-n-methylneamine and gentamine c2, respectively. these amtibiotics were characterized as 6'-n-mentylbutirosins a and b (nmb-a & nmb-b), and 3', 4'-dideoxy-6'-c-methylbutirosins a and b (dcb-a & dcb-b), respectively, by chemical and spectroscopic studies. nmb-a and nmb-b exhibited broad- ... | 1978 | 81824 |
| sodium-dependent transport of l-leucine in membrane vesicles prepared from pseudomonas aeruginosa. | membrane vesicles were prepared by osmotic lysis of spheroplasts of pseudomonas aeruginosa strain p14, and the active transport of amino acids was studied. d-glucose, gluconate, and l-malate supported active transport of various l-amino acids. the respiration-dependent leucine transport was markedly stimulated by na+. moreover, without any respiratory substrate, leucine was also transported transiently by the addition of na+ alone. this transient uptake of leucine was not inhibited either by car ... | 1979 | 83991 |
| [principle of constructing polyvalent pseudomonas aeruginosa vaccines]. | dependence of the range of protective action of p. aeruginosa vaccine on the number of its composites was studied. a principle of the selection of strains who vaccines differed in vivo by immunological specificity was applied to construction of the experimental preparations and modelling a polyvalent vaccine. increase of the number of components in the vaccine was accompanied by increase of its protective action range. however, with the increase of the number of polyvaccine components in the pol ... | 1979 | 84457 |
| [the catabolism of arginine in pseudomonas aeruginosa (proceedings)]. | 1978 | 84621 | |
| [the differentiation of oep-ha titer between gamma-globulin products for intravenous use and their effectiveness in the passive protection of mice against pseudomonas aeruginosa infection (author's transl)]. | 1978 | 85678 | |
| isolation of inc p-2 plasmid dna from pseudomonas aeruginosa. | 1978 | 86192 | |
| [use of simmons' medium for isolating pseudomonas aeruginosa from associations with proteus]. | 1979 | 86654 | |
| the study of antigenic relationships between bacteriophages of the romanian lysotyping scheme for pseudomonas aeruginosa. | 1978 | 87183 | |
| [therapeutic effects of anti-oep-antibodies in the pseudomonas aeruginosa (fisher's seven serotypes) infection of mice and the influence of this antibody upon the phagocytic activity of mice spleen macrophages (author's transl)]. | 1979 | 89175 | |
| experimental studies of the pathogenesis of infections due to pseudomonas aeruginosa: extracellular protease and elastase as in vivo virulence factors. | the effects on mortality of supplemental injections of protease and elastase were determined in burned mice infected with non-lethal inocula of a toxin-producing but non-proteolytic-enzyme-producing strain of pseudomonas aeruginosa. when a variety of solutions containing proteolytic enzyme were injected under these conditions, the mortality increased significantly. this did not occur when organisms other than p. aeruginosa were used. injections of the enzyme solutions alone were non-lethal. inje ... | 1979 | 89891 |
| an acellular vaccine from pseudomonas aeruginosa: homologous and crossed protection between serogroups according to habs' classification. | homologous and cross-reactions were studied in 16 strains of pseudomonas aeruginosa belonging to different o serogroups according to habs' classification. by slide agglutination, cross-reactions were rare between the strains of two or three serogroups: o2, o5 and o16; o1 and o9; o7 and o8; o13 and o14. experiments of cross-protection have been performed in mice inoculated with acellular vaccines prepared from the 16 studied strains. a high homologous protection was observed with all the 16 strai ... | 1979 | 90473 |
| [immunologic study of the cellular components of bacillus pyocyaneus. v. antigenic analysis of slime and its fractions]. | various slime fractions were obtained from newly isolated mucoid strains of p. aeruginosa by the method of ultrafiltration or differential centrifugation with subsequent gel chromatography. purified slime was found to react with a broader spectrum of typing o sera than the corresponding cell wall lipopolysaccharides. erythrocytic diagnostic preparations produced on the basis of slime antigens allowed to reveale the presence of circulating antibodies against p. aeruginosa. the slime components wi ... | 1979 | 91285 |
| controlled trials of a polyvalent pseudomonas vaccine in burns. | a polyvalent pseudomonas vaccine has been tested in controlled clinical trials at two burns units, in birmingham and new delhi, in children and adults with burns more than 15% full skin thickness. none of the vaccinated patients in either trial showed blood cultures containing pseudomonas aeruginosa, and vaccinees showed raised titres of protective antibody and increased phagocytic activity against ps. aeruginosa. in the new delhi unit, where death from ps. aeruginosa infection is common, the mo ... | 1979 | 91774 |
| transfer of rp4 and r68.45 factors to rhizobium. | two r factor were introduced by conjugation into rhizobium trifolii and rhizobium meliloti strains at a frequency of 10(-5) to 10(-6). plasmids rp4 from escherichia coli j53 and r68.45 from pseudomonas aeruginosa pao.25 were maintained stably in rhizobium hosts and could be retransferred to other rhizobium recipients. some of the transconjugants were able to mobilize chromosome and transfer his or met genes in intra-, and interspecies matings. | 1979 | 92171 |
| an in vitro study on the effect of benzoyl peroxide 20%, gentamicin and phenoxyethanol on staphylococcus aureus, pseudomonas aeruginosa and proteus species. | 1979 | 94224 | |
| heat-stable somatic antigens of a group of unclassified fluorescent pseudomonads (ufp). | (i) isolates belonging to a group of unclassified fluorescent pseudomonads (ufp) are similar to pseudomonas aeruginosa not only in cultural behaviour but also in basic serological properties of their somatic antigens. living bacteria and cultures subjected to prolonged heating at 100 degrees c or above agglutinated readily in homologous o serum, whereas after exposure to 60 degrees c, ethanol, saturated sodium chloride and formalin the cells of both species became practically inagglutinable. sur ... | 1979 | 94240 |
| [endotoxic contamination of biological products (ribosomal vaccines, viral vaccines and interferon)]. | the a.a. have examined by the limulus assay the possible endotoxin contamination in some biological products (ribosomal vaccines, viral vaccines, interferon). while the preparations of influenza vaccines and a partially purified fraction of ribosomal vaccine from pseudomonas aeruginosa exhibit a gelation of lysate with high levels of endotoxin, rubeola vaccines, interferon and a purified fraction of ribosomal vaccine, presented a negligible amount of endotoxin. the results are discussed with the ... | 1979 | 95449 |
| mezlocillin: a new acyl ureidopenicillin. antimicrobial activity and combination effects with four aminoglycoside antibiotics. | mezlocillin (baypen), carbenicillin and mezlocillin combined with gentamicin, sisomicin, tobramycin and amikacin, respectively, were tested in vitro against 325 fresh clinical isolates of pathogenic gram-negative bacteria using the tube dilution procedure. the new acyl ureidopenicillin has a greater antimicrobial activity than carbenicillin against nearly all species tested. the comparison of the effectiveness of the mezlocillin-aminoglycoside combinations demonstrates the superiority of mezloci ... | 1978 | 95925 |
| biosynthesis of exopolysaccharide by pseudomonas aeruginosa. | in batch cultures of pseudomonas aeruginosa, the maximum rate of exopolysaccharide synthesis occurred during exponential growth. in nitrogen-limited continuous culture, the specific rate of exopolysaccharide synthesis increased from 0.27 g g of cell-1 h-1 at a dilution rate (d) of 0.05 h-1 to 0.44 g g of cells h-1 at d=0.1 h-1. the yield of exopolysaccharide on the basis of glucose used was in the range of 56 to 64%. exopolysaccharide was also synthesized in carbon-limited cultures at 0.19 g g o ... | 1978 | 96088 |
| pattern of phenazine pigment production by a strain of pseudomonas aeruginosa. | an atypical strain of pseudomonas aeruginosa capable of synthesizing three phenazine pigments was isolated. cultural conditions, under which the strain forms either chlororaphin, oxychlororaphin, or pyocyanine, are described. this broad spectrum of pigment production, as well as some other characteristics, sets this strain apart from previously described chlororaphin producers. | 1978 | 96094 |
| n-terminal amino acid sequence of pilin isolated from pseudomonas aeruginosa. | the amino-terminal amino acid sequence of the pili protein from pseudomonas aeruginosa k pili is presented. the sequence is compared with those reported by others for pilin obtained from neisseria gonorrhoeae and moraxella nonlique-faciens. all three sequences are highly homologous, contain only two hydrophilic residues in the first 22 positions, and contain an unusual amino acid, n-monomethylphenylalanine, at the amino terminus. | 1978 | 96098 |
| characterization of pseudomonas aeruginosa mutants deficient in the establishment of lysogeny. | mutants of pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (les-) have been isolated. these les mutations map to two areas of the p. aeruginosa chromosomal map as determined by conjugational and transductional analyses. two phenotypic classes of les- mutants were identified. one class of mutations has pleiotropic effects on dna metabolism. these mutants are unable to recombine genetic material acquired as a result of either conjugat ... | 1978 | 96103 |
| pathogenesis of pseudomonas aeruginosa pneumonia during immunosuppression. | a guinea pig model of immunosuppression was utilized to study the effects of immunosuppressive chemotherapy on lung response to challenge with pseudomonas aeruginosa. study groups included normal guinea pigs, as well as guinea pigs that received a one-week course of cortisone acetate (ca, 100 mg/kg per day) plus 15 mg of cyclophosphamide (ctx)/kg per day (ca + loctx group) or 30 mg of cyclophosphamide/kg per day (ca + hictx group). separate groups received ca or hictx alone. intratracheal instil ... | 1978 | 96188 |
| methods for storage of antimicrobial effectiveness test inoculum suspensions below freezing. | studies were conducted to determine the suitability of storage below freezing of some antimicrobial effectiveness test inoculum organisms: staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and candida albicans. several solutions used for protecting microorganisms subjected to storage below freezing were compared. comparison of 10% dextrose with other solutions (distilled water; 0.07 m phosphate buffer, h 7.0, with 15% glycerol; and 0.07 m phosphate buffer, ph 7.0, with 7.5% dimeth ... | 1978 | 96247 |
| experimental studies of the pathogenesis of pseudomonas aeruginosa infection: evidence for the in-vivo production of a lethal toxin. | evidence is presented that a lethal toxin is produced by p. aeruginosa growing in the burned skin of experimental mice. after injection of approximately 100 p. aeruginosa cells into the burned skin there was a rapid proliferation of organisms at the site of inoculation. when the organisms in the burned skin tissue reached a critical concentration, there was generalised toxaemia with subsequent mortality; the process was not reversible at this stage, even by reducing substantially the numbers of ... | 1978 | 96255 |
| experimental infections with protease-deficient mutants of pseudomonas aeruginosa in mice. | the virulence of a protease-producing strain of psuedomonas aeruginosa was compared with that of mutants that had lost the ability to produce proteases and other extracellular enzymes. lethal infections were produced by inoculating mice intraperitoneally with bacteria in mucin, or by inoculating mice intraperitoneally or intravenously with bacteria 4 days after treatment with cyclophosphamide, 200 mg per kg body weight. no significant difference in virulence between the wild-type parent strain a ... | 1978 | 96256 |
| pseudomonas colonization in cystic fibrosis. a study of 160 patients. | we investigated the role of pseudomonas aeruginosa colonization in the respiratory tracts of cystic fibrosis (cf) patients to relate the effect of this colonization to progression of bronchial airway pathologic conditions and to the patients' clinical progress, and to identify predisposing factors to persistence of p aeruginosa colonization and bronchial tree damage. half of 160 cf patients studied had persistent p aeruginosa respiratory tract colonization; the other half had none. pseudomonas a ... | 1978 | 96277 |
| [management of patients with pseudomonas aeruginosa infections]. | 1978 | 96314 | |
| comparative in-vitro activity of ticarcillin and carbenicillin against 100 clinical isolates of pseudomonas aeruginosa. | the in-vitro activity of ticarcillin was found to be greater than that of carbenicillin against 97 out of 100 strains of pseudomonas aeruginosa which were recently isolated from clinical specimens at the royal perth hospital complex. these results indicate that ticarcillin should be considered for use in place of carbenicillin for treating infections caused by p. aeruginosa. | 1978 | 96319 |
| pseudomonas cytochrome c551 at 2.0 a resolution: enlargement of the cytochrome c family. | the structure of respiratory cytochrome c551 of pseudomonas aeruginosa, with 82 amino acids, has been solved by x-ray analysis and refined to a crystallographic r factor of 16.2%. it has the same basic folding pattern and hydrophobic heme environment as cytochromes c, c2, and c550, except for a large deletion at the bottom of the heme crevice. this same "cytochrome fold" appears to be present in photosynthetic cytochromes c of green and purple sulfur bacteria, and algal cytochromes f, suggesting ... | 1978 | 96440 |
| [immunologic study of the cellular components of bacillus pyocyaneus. iii. immunochemical analysis, toxicity and protective properties of water-soluble antigenic complexes]. | aqueous extracts of two ps. aeruginosa strains killed with acetone were subjected to fractionation by preparative ultracentrifugation and gel-chromatography. toxic activity of the extract was found to be connected with the high-molecular, possibly glycoprotein components reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 30--40% of rats against the generalized infection with pseudomonas aeruginosa. this protective activity is apparently connected with the p ... | 1978 | 96637 |
| factors associated with acquisition of pseudomonas aeruginosa resistant to gentamicin. | concern over the increased occurrence of pseudomonas aeruginosa resistant to gentamicin colonizing and/or causing disease in patients prompted our review of the years 1974 and 1975 for all p aeruginosa isolates, both gentamicin-resistant and gentamicin-sensitive. in this period, 39 patients had gentamicin-resistant p aeruginosa recovered from clinical specimens while 683 patients had gentamicin-sensitive strains. compared to both matched and/or randomly selected controls with gentamicin-sensitiv ... | 1978 | 96695 |
| activity of azlocillin and mezlocillin against gram-negative organisms: comparison with other penicillins. | the activities of azlocillin and mezlocillin were compared with those of carbenicillin, ticarcillin, and pirbenicillin against a wide range of gram-negative organisms. the two new drugs were considerably more active than carbenicillin against klebsiella species and escherichia coli. carbenicillin was twice as active against proteus mirabilis as mezlocillin and four times as active as azlocillin. against pseudomonas aeruginosa, azlocillin was eight times as active as carbenicillin. azlocillin and ... | 1978 | 96726 |
| plasmid-determined resistance to hexachlorophene in pseudomonas aeruginosa. | plasmid-containing strains of pseudomonas aeruginosa were screened for resistance to disinfectants. two plasmids, pmg1 and pmg2, were found to determine hexachlorophene resistance. | 1978 | 96729 |
| plasmid-determined resistance to boron and chromium compounds in pseudomonas aeruginosa. | plasmids determining resistance to arsenic, mercury, silver, and tellurium compounds in escherichia coli and pseudomonas aeruginosa were tested for resistance to 40 other metal compounds. resistance to trivalent boron and hexavalent chromium compounds was a property of certain p. aeruginosa plasmids. | 1978 | 96730 |
| beta-lactamases: determination of their isoelectric points. | important discrepancies in isoelectric points (pi) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (ief) in sucrose density gradients or analytical ief in thin-layer polyacrylamide gels (pa). the variations, negligable in the case of tem-like beta-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in pa-ief. this has been clearly shown with beta-lactamase pr ... | 1978 | 96731 |
| relationship between gentamicin susceptiblity criteria and therapeutic serum levels for pseudomonas aeruginosa in mouse infection model. | in this study estimations of in vivo and in vitro gentamicin susceptibility for a series of strains of pseudomonas aeruginosa were compared. the series included an extremely susceptible strain, typically susceptible strains by current susceptibility criteria, and strains with enzymatic and permeability-mediated resistance. in vivo testing was done by using a mice protection test involving six 1-h doses of gentamicin and an inoculum of 50 50% lethal doses of p. aeruginosa. both normal mice and cy ... | 1978 | 96735 |
| unpredictable response of pseudomonas aeruginosa to synergistic antibiotic combinations in vitro. | the combined activity of ticarcillin and each of three aminoglycosides, gentamicin, tobramycin, and amikacin, was studied with 45 clinical isolates of pseudomonas aeruginosa. all strains individually were susceptible to each of these agents. comparison of the results revealed highly variable interaction, both from strain to strain and from one aminoglycoside to another against the same strain. against 11 strains, none of three antibiotic pairs tested was synergistic by conventional criteria; aga ... | 1978 | 96736 |
| antigenic variation and increase in pathogenicity in pseudomonas aeruginosa as a result of growth on glucose or n-hexadecane as sole carbon source. | when pseudomonas aeruginosa is grown on glucose as opposed to n-hexadecane as the sole carbon source, the antigenicity, virulence, and protein composition of the outer membrane are altered. the hydrocarbon-grown cells demonstrate a 3-log increase in virulence over the glucose-grown cells (in mice). there also appears to be an additional protein present in the outer membrane of the n-hexadecane-grown cells. this protein may contribute to the observed antigenic differences between the two cell typ ... | 1978 | 96928 |
| synergy studies with pseudomonas aeruginosa resistant to gentamicin and/or carbenicillin. | the predictability of synergy with strains of pseudomonas aeruginosa highly resistant to gentamicin in combination with carbenicillin has been controversial. 30 clinical isolates of p. aeruginosa resistant to gentamicin and/or carbenicillin were tested by checker-board technique. 14 were found to be highly resistant to gentamicin (minimal inhibitory concentration mic greater than or equal to 128 microgram/ml) and/or carbenicillin (mic greater than or equal to 512 microgram/ml). of these 14, 4 is ... | 1978 | 97057 |
| effect of growth environment on pseudomonas aeruginosa killing by rabbit polymorphonuclear leudocytes and cationic proteins. | pseudomonas aeruginosa grown in a chemostat under carbon- and magnesium-limited conditions showed varying resistance to killing by rabbit peritoneal exudate polymorphonuclear leukocytes. slow-growing (d = 0.05 h-1), magnesium-limited cells were significantly more resistant to the lethal effects of the phagocytes than were fast-growing magnesium-limited cells and carbon-limited cells (d = 0.05 h-1 and d = 0.5 h-1, respectively). the resistance of magnesium-limited cells to killing by cationic pro ... | 1978 | 97227 |
| cigarette smoke and phagocyte function: effect of chronic exposure in vivo and acute exposure in vitro. | phagocytic function was studied in mice chronically exposed to cigarette smoke, and the effects of in vitro exposure to cigarette smoke on macrophage activity were also assessed. cultures of radiolabeled pseudomonas aeruginosa were employed to investigate phagocyte activity in vivo and in vitro. mice were exposed on weekdays to fresh cigarette smoke for periods up to 37 weeks and the bactericidal and clearance activity of their lungs was measured. both pulmonary clearance and bactericidal activi ... | 1978 | 97229 |
| mucoid pseudomonas aeruginosa and cystic fibrosis: resistance of the mucoid from to carbenicillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro. | 1978 | 97259 | |
| isolation of large bacterial plasmids and characterization of the p2 incompatibility group plasmids pmg1 and pmg5. | large plasmids from agrobacterium tumefaciens, salmonella typhimurium, escherichia coli, pseudomonas putida, and pseudomonas aeruginosa were routinely and consistently isolated using a procedure which does not require ultracentrifugation but includes steps designed to separate large-plasmid dna from the bacterial folded chromosome. it also selectively removes fragments of broken chromosome. a variety of large plasmids was readily visualized with agarose gel electorphoresis, including five betwee ... | 1978 | 97269 |
| tol is a broad-host-range plasmid. | we readily isolated insertions of the carbenicillin resistance element tn401 into the tol plasmid in pseudomonas putida. hybrid tol::tn401 plasmids stably express the cbr phenotype in pseudomonas aeruginosa and escherichia coli. whereas the replicative and conjugative functions are expressed in both hosts, the ability to grow on m-toluate is only expressed in the pseudomonas species. | 1978 | 97271 |
| lymphocyte responsiveness to pseudomonas aeruginosa in cystic fibrosis: relationship to status of pulmonary disease in sibling pairs. | lymphocyte proliferative responses to pseudomonas aeruginosa and staphylococcus aureus were evaluated in six sibling pairs with cystic fibrosis. in each pair, one sibling had advanced clinical disease, whereas the other sibling was in good clinical condition. three in this latter group had no clinically apparent pseudomonas bronchitis. in all cases, the average responses to pseudomonas isolated from each sibling pair were lower in the sibling with advanced clinical disease. this difference was n ... | 1978 | 97373 |
| therapeutic effect of immunization with oep, protease toxoid and elastase toxoid on corneal ulcers in mice due to pseudomonas aeruginosa infection. | the effectiveness of immunizing mice with protease toxoid (pt) or elastase toxoid (et) on the corneal ulcerization due to either protease or elastase were investigated. consequently, mice immunized with either pt or et were protected from corneal ulcers experimentally induced by the homologous enzyme, either protease or elastase. similarly, two kinds of rabbit immune sera, anti-pt and anti-et, were found to prevent corneal ulcers by the homologous enzyme. then, the therapeutic effects of vaccina ... | 1978 | 97424 |
| phosphoramidon as an inhibitor of elastase from pseudomonas aeruginosa. | 1978 | 97425 | |
| [sensitivity of bacteria to antibiotics. evaluation of a rapid automatic method (author's transl)]. | a rapid automated sensitivity testing system is evaluated in this report. the results have been compared with the standard disk diffusion method (ics). three hundred strains recently isolated from clinical cases : enterobacteriaceae, staphylococcus aureus, streptococci d, pseudomonas aeruginosa, have been tested. the general agreement between the two techniques was 89 p. cent. | 1978 | 97613 |
| [immunological study of cellular components of pseudomonas aeruginosa. iv. fractionation of pseudomonas aeruginosa mucus by diafiltration and biological properties of isolated fractions]. | in fractionation of pseudomonas aeruginosa mucus (strains no. 8 and 1463) by means of diafiltration on the system of membranes diaflo xm-300, xm-100a, pm-30, and pm-10 there was obtained a successive series of fractions differing by the molecular weight and chemical composition. according to the results of gel chromatography fractions with the mol wt of 100000 dalton and over apparently represented protein-polysaccharide components of mucus in the form of complexes; fractions with the mol wt of ... | 1978 | 97882 |
| chymotrypsinlike activity of some intestinal bacteria. | some tests of exocrine pancreatic function are based on the estimation of intestinal chymotryptic activity by using specific substrates such as n-benzoyl-l-tyrosyl-paba (bt-p). in this study the action of a number of bacteria isolated from human or monkey intestines on bt-p was investigated. the monkeys studied had an intestinal flora closely resembling that of man. except for bacteroides, proteus vulgaris no. 73, proteus morgani no. 202, and pseudomonas aeruginosa no. 76, significant splitting ... | 1978 | 98039 |
| [incidence of the "o" groups of "pseudomonas aeruginosa" in the province of valencia (spain) (author's transl)]. | 1978 | 98085 | |
| infection in acute leukemia patients receiving oral nonabsorable antibiotics. | during a 20-month period all acute nonlymphocytic patients (87 patient trials) receiving cytotoxic chemotherapy were placed on an oral nonabsorbable antibiotic regimen consisting of gentamicin, vancomycin, and nystatin in addition to an intensive program of infection prevention aimed at reducing exogenously acquired and body-surface potential pathogens. although side effects of anorexia, diarrhea, and nausea were common, gentamicin-vancomycin-nystatin was ingested 80% of the study time. microbia ... | 1978 | 98107 |
| effect of triethylenetetramine dihydrochloride on the antibiotic susceptibility of pseudomonas aeruginosa. | a chelating agent, triethylenetetramine dihydrochloride, interacted synergistically in vitro with both gentamicin and carbenicillin against a clinical isolate of pseudomonas aeruginosa designated ps 15. the minimal inhibitory concentrations of carbenicillin and gentamicin for ps 15 in a 50% serum-trypticase soy broth were 250 and 72.9 mug/ml, respectively. however, addition of triethylenetetramine dihydrochloride to the 50% serum-trypticase soy broth reduced the minimal inhibitory concentration ... | 1978 | 98108 |
| in vitro antibacterial activity of amikacin and ticarcillin, alone and in combination, against pseudomonas aerurginosa. | in vitro antimicrobial susceptibility studies using amikacin and ticarcillin, alone and in combination, were performed on 20 strains of pseudomonas aeruginosa. all strains were susceptible to amikacin, and ticarcillin was active against 16 of the 20 strains. enhanced anti-pseudomonas activity could be demonstrated with the combination of amikacin and ticarcillin. | 1978 | 98109 |
| ocular flora in the severely burned patient. | one hundred six eyes of 53 patients with severe thermal burns were studied with serial conjunctival cultures during a four-month period. a dramatic shift of the normal conjunctival flora from the preponderance of staphylococcus epidermidis and corynebacterium to s aureus and gram-negative bacilli was observed. this commonly occurred on the fifth to sixth postburn day and represented colonization of the conjunctiva by bacteria recovered from other sites in the body. despite the high incidence of ... | 1978 | 98138 |
| positive regulation of amidase synthesis in pseudomonas aeruginosa. | mutants of pseudomonas aeruginosa were isolated that were acetamide-negative in growth phenotype at 41 degrees c and constitutive for amidase synthesis at 28 degrees c. two mutants were derived from the magno-constitutive amidase mutant pac111 (c11), and a third from a mutant that had enhanced inducibility by formamide, pac153 (f6). the three temperature-sensitive mutants produced amidases with the same thermal stabilities as the wild-type enzyme. cultures growing exponentially at 28 degrees c, ... | 1978 | 98516 |
| antibodies to proteases and exotoxin a of pseudomonas aeruginosa in patients with cystic fibrosis: demonstration by radioimmunoassay. | sera from 33 patients with cystic fibrosis and two pediatric patients being treated for chronic pulmonary infections not related to cystic fibrosis and six sera or serum pools from uninfected individuals were tested with a microtiter radioimmunoassay for reactivity against exotoxin a and two proteases from pseudomonas aeruginosa. exotoxin a was purified from a low-protease strain of p. aeruginosa and shown to have adenosine diphosphate-ribose transferase activity and mouse lethality. proteases w ... | 1978 | 98596 |
| [pseudomonas aeruginosa infection in patients with thermal lesions]. | 1978 | 98664 | |
| [effect of pseudomonas aeruginosa melanin on antibiotic activity]. | the properties of microbial melanines are very diverse. melanine of p. aeruginosa is little studied. the pigment was isolated from a strain of p. aeruginosa possessing all characteristic properties of the species. interaction of p. aeruginosa melanine with various antibiotics was determined by the method of serial dilutions in beaf-peptone broth, using staph. aureus 209 as a test-microbe, which was added to the medium in an amount of 10(6) cells to each tube. it was found that p. aeruginosa mela ... | 1978 | 99081 |
| comparison of silver sulfadiazine and gentamicin for topical prophylaxis against burn wound sepsis. | daily prophylactic application of either 1.0% silver sulfadiazine cream or 0.1% gentamicin cream was compared for effectiveness in preventing bacterial colonization of burn wounds and sepsis. pseudomonas aeruginosa colonized the wounds of 37% of the 38 patients treated with silver sulfadiazine and 30% of the 33 patients treated with gentamicin; gentamicin-resistant p. aeruginosa colonized the wounds of 21% of the patients treated with gentamicin. staphylococcus aureus colonization occurred in 55 ... | 1978 | 99223 |
| in vitro effect of purified proteases of pseudomonas aeruginosa on rabbit lung macrophages. | 1978 | 99330 | |
| time and dose studies of the effect of cobra venom factor on the in vivo immune response in galleria mellonella to pseudomonas aeruginosa. | the induced resistance of wax moth larvae vaccinated with formolized pseudomonas aeruginosa 18 hr prior to challenge with the live organism was significantly inhibited by 0.2 to 1.0 units of cobra venom factor (cvf) per insect given 6 hr after vaccination. approximately 90% inhibition of the protective response was caused by 1.0 units cvf/insect. administration of this dose 6 hr before vaccination, with the vaccine, and 6 hr before challenge had no significant effect. the cvf effect was inhibite ... | 1978 | 99334 |
| depression of contact sensitivity by enhancement of suppressor cell activity in pseudomonas aeruginosa-injected mice. | heat-killed pseudomonas aeruginosa depresses contact sensitivity to oxazolone in c56bl/6 mice. the draining lymph nodes and spleens of mice exhibiting an impaired reactivity to oxazolone contain a cell population capable of depressing the response to oxazolone of recipients sensitized immediately before cell transfer. the suppressive activity of these cells appears to be antigen specific, since they do not affect the response to picryl chloride and because they do not arise in p. aeruginosa-inje ... | 1978 | 99365 |
| transfer-deficient mutants of the narrow-host-range plasmid r91-5 of pseudomonas aeruginosa. | three methods have been successful in the isolation of transfer-deficient mutants of the narrow-host-range r plasmid r91-5 of pseudomonas aeruginosa: (i) selection for donor-specific phage resistance; (ii) direct screening after mutagenic treatment with either ethyl methane sulfonate or n-methyl-n'-nitro-n-nitrosoguanidine; (iii) in vitro mutagenesis of plasmid dna by hydroxylamine followed by transformation and direct screening. the majority of transfer-deficient mutants were donor-specific pha ... | 1978 | 99439 |
| separation and characterization of the outer membrane of pseudomonas aeruginosa. | a method is described for the preparation of outer and cytoplasmic membranes of pseudomonas aeruginosa, and the outer membrane proteins characterized. isolated outer and cytoplasmic membranes differed markedly in the content of 2-keto-3-deoxyoctonate (lipopolysaccharide) and phospholipid as well as in the localization of certain enzymes (nadh oxidase, succinate dehydrogenase, d-lactate dehydrogenase, malate dehydrogenase, and phospholipase), and also in the microscopic morphology. the outer memb ... | 1978 | 99443 |
| evaluation of some methods for the laboratory examination of sputum. | methods for the quantitation of leucocytes, squamous epithelial cells, and potential pathogens in sputa are described. microscopic examination showed that 58% of sputa tested (554/957) were purulent or moderately purulent and 48% were moderately or heavily contaminated by squamous epithelial cells. the presence of squamous epithelial cells indicated oropharyngeal contamination. a simple dilution technique was chosen to compare the isolation of potential pathogens from direct cultures and from di ... | 1978 | 99457 |
| r plasmids which alter ultraviolet light-sensitivity and enhance ultraviolet light-induced mutability in pseudomonas aeruginosa. | r plasmids pmg1, r2, r931 and pmg15 increased the survival of pseudomonas aeruginosa exposed to ultraviolet radiation (u.v.) in the wild type, and uvr and pola mutants but did not alter the u.v.-response of a reca mutant. the r plasmid rpl11 reduced u.v.-survival in the wild type, and uvr and pola mutants but did not alter the u.v.-response of a reca host. all the plasmids enhanced the level of spontaneous and u.v.-induced back mutation (trp+) in a trpb1 strain. the effect of sublethal concentra ... | 1978 | 99485 |
| [comprehensive characterization of pseudomonas aeruginosa strains with particular reference to the habs and homma serological typing methods]. | 1978 | 99734 | |
| [the antibacterial activity of diphenhydramine (author's transl)]. | diphenhydramine (n,n-dimethyl-2-diphenyl-methoxy-ethylamine) [n,n-dimethyl-2-(diphenylmethoxy)-ethylamin] (dph) is a well-known local anaesthetic and an antagonist of both acetylcholine and histamine. we tested its antibacterial efficacy by means of three different investigation methods, i.e. microcalorimetry, continuous density measurement and the microdilution test to find out the mic of this compound against various bacteria. dph inhibits the growth of e. coli and klebsiella strains at a conc ... | 1978 | 99881 |
| uk-18892, a new aminoglycoside: an in vitro study. | uk-18892 is a new aminoglycoside antibiotic, a derivative of kanamycin a structurally related to amikacin. it was found to be active against a wide range of pathogenic bacteria, including many gentamicin-resistant strains. the spectrum and degree of activity of uk-18892 were similar to those of amikacin, and differences were relatively minor. uk-18892 was about twice as active as amikacin against gentamicin-susceptible strains of pseudomonas aeruginosa. both amikacin and uk-18892 were equally ac ... | 1978 | 100050 |
| modified peptidoglycan transpeptidase activity in a carbenicillin-resistant mutant of pseudomonas aeruginosa 18s. | a carbenicillin-resistant mutant of pseudomonas aeruginosa 18s was found to possess peptidoglycan transpeptidase activity significantly more resistant to inhibition by benzyl penicillin, ampicillin, carbenicillin, and cephaloridine than that of the parent strain. the mutant was more resistant than the parent strain to all of the beta-lactam antibiotics tested, and 50% inhibition values for these compounds against membrane-bound model transpeptidase activity paralleled this increase. the resistan ... | 1978 | 100051 |