Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| improved medium for recovery and enumeration of pseudomonas aeruginosa from water using membrane filters. | a modified mpa medium, designated mpa-c, was shown to recover pseudomonas aeruginosa from a variety of water sources with results comparable to those with mpa-b and within the confidence limits of a most-probable-number technique. enumeration of p. aeruginosa on mpa-c was possible after only 24 h of incubation at 41.5 degrees c, compared with 72 h of incubation required for mpa-b and 96 h of incubation for a presumptive most probable number. | 1978 | 100052 |
| automated food microbiology: potential for the hydrophobic grid-membrane filter. | bacterial counts obtained on hydrophobic grid-membrane filters were comparable to conventional plate counts for pseudomonas aeruginosa, escherichia coli, and staphylococcus aureus in homogenates from a range of foods. the wide numerical operating range of the hydrophobic grid-membrane filters allowed sequential diluting to be reduced or even eliminated, making them attractive as components in automated systems of analysis. food debris could be rinsed completely from the unincubated hydrophobic g ... | 1978 | 100054 |
| denitrifying pseudomonas aeruginosa: some parameters of growth and active transport. | optimal cell yield of pseudomonas aeruginosa grown under denitrifying conditions was obtained with 100 mm nitrate as the terminal electron acceptor, irrespective of the medium used. nitrite as the terminal electron acceptor supported poor denitrifying growth when concentrations of less than 15 mm, but not higher, were used, apparently owing to toxicity exerted by nitrite. nitrite accumulated in the medium during early exponential phase when nitrate was the terminal electron acceptor and then dec ... | 1978 | 100056 |
| in vitro and in vivo effect of pc 904, a new broad-spectrum penicillin, on ocular strains of pseudomonas aeruginosa. | the in vitro and in vivo activities of pc 904 were tested against pseudomonas aeruginosa isolated from infected eyes and were compared with carbenicillin and gentamicin. by the microtiter plate method, we tested 30 strains of p. aeruginosa in vitro; pc 904 was very active against 29, but one strain was resistant. in a comparative study, pc 904 was more active than carbenicillin but less active than gentamicin. in vivo tests were conducted on rabbit corneas infected with a strain of p aeruginosa ... | 1978 | 100194 |
| in vitro activity of carbenicillin, ticarcillin, sisomicin and netilmicin alone and in combination against pseudomonas aeruginosa. | the in vitro activity of carbenicillin, ticarcillin, sisomicin and netilmicin alone and in combination against 35 strains of pseudomonas aeruginosa was investigated. ticarcillin was more active than carbenicillin and sisomicin was more active than netilmicin. there was enhanced killing of many strains of p. aeruginosa by the combination of carbenicillin or ticarcillin with sisomicin or netilmicin. | 1978 | 100282 |
| comparative study of the sensitivity of acetylcholinesterases and cholinesterases from animal and bacterial sources to inhibition by serotonin and its derivatives. | serotonin was found to inhibit human erythrocyte and electric-eel acetylcholinesterase activities. the serotonin amino group, free of negative charges in its vicinity and its hydroxyl group, were important for the inhibition. serotonin precursors and several related compounds had little or no effect. human plasma cholinesterase was also inhibited by serotonin and tryptamine. in contrast to these animal enzymes, the cholinesterase of pseudomonas aeruginosa was refractory to serotonin and its deri ... | 1978 | 100343 |
| susceptibility of 327 clinical isolates to netilmicin. | netilmicin, a semisynthetic derivative of sisomicin, was tested against 327 isolates of staphylococcus aureus, pseudomonas aeruginosa and gram-negative enteric bacilli. seventy-two per cent of the isolates were inhibited at a concentration of 0.5 microgram netilmicin per ml, and 93% of the isolates were susceptible to 4 microgram per ml or less. the mics of netilmicin and gentamicin for 24 providencia and 38 pseudomonas isolates were compared. the activity of netilmicin closely paralleled that o ... | 1978 | 100353 |
| a comparison of the antibiotic activities of sisomicin, gentamicin and aminosidine sulphate against pseudomonas aeruginosa. | sisomicin, gentamicin and aminosidine sulphate were compared for their antibiotic activity against pseudomonas strains freshly isolated from clinical material. the activity of the three agents, expressed as a minimum inhibitory concentration, was determined using the same automatized system. | 1978 | 100354 |
| pathogenesis of experimental pseudomonas keratitis in the guinea pig: bacteriologic, clinical, and microscopic observations. | uniformly severe corneal infections were produced in guinea pigs by intracorneal injection of about 10 viable pseudomonas aeruginosa. after a brief lag period, multiplication of bacteria was rapid, reaching geometric means of 280,000 after 24 hr and of 5 million after 48 hr. within 8 hr after inoculation, polymorphonuclear leukocytes (pmns) began to infiltrate the anterior two thirds of the stroma. stromal cells adjacent to the injection site became necrotic and appeared to be engulfed by pmns. ... | 1978 | 100468 |
| serological classification of pseudomonas aeruginosa by a slide agglutination test. | serological classification of pseudomonas aeruginosa by the slide agglutination test with live organisms was studied, based on the o antigen schema adopted by the international expert panel sponsored by the subcommittee on pseudomonas and related organisms of the international committee on systematic bacteriology. the typing results obtained by the slide test with well-absorbed o sera were identical to those obtained by the conventional tube agglutination test with autoclaved organisms. most o a ... | 1978 | 100507 |
| hygiene in the prevention of udder infections. iv. evaluation of teat dips with excised cows' teats. | suspensions of staphylococcus aureus, streptococcus agalactiae, escherichia coli, and pseudomonas aeruginosa were used to contaminate teats excised from cows. commercially available teat dips were applied for evaluation of comparative germicidal activity. iodophors, sodium hypochlorite, and sodium dichloro-s-triazenetrione were highly effective against all four test organisms. quarternary ammonium, chlorhexidine, and cetylpyridinium chloride were effective against staphylococcus aureus, and stre ... | 1978 | 100535 |
| [epidemiological study of the early intestinal colonization with pseudomonas pyocyanea in 2 neonatal departments. preliminary note]. | 1978 | 100671 | |
| [appearance of various pyocin types and subtypes of pseudomonas aeruginosa in the same patient]. | 1978 | 100965 | |
| inability of the standardized disk agar-diffusion test to measure susceptibility of the fluorescent group of pseudomonads to gentamicin. | four hundred thirteen clinical isolates of fluorescent pseudomonads consisting of 286 pseudomonas aeruginosa and 127 organisms of the pseudomonas fluorescent group were tested for susceptibilities to gentamicin by the broth-dilution method and by the standardized disk agar-diffusion test, using mueller-hinton agar from three sources. different regression lines and significantly different mean inhibition zone diameters were found for the three mueller-hinton agars. single zone-diameter breakpoint ... | 1978 | 101077 |
| beta-lactamase stability of hr 756, a novel cephalosporin, compared to that of cefuroxime and cefoxitin. | the stability to beta-lactamase hydrolysis of hr 756, a new cephalosporin antibiotic, was compared to the beta-lactamase stability of cefoxitin and cefuroxime. hr 756, cefoxitin, and cefuroxime were not hydrolyzed by richmond type i, iii, iv, and v beta-lactamases. antibacterial activity of hr 756 correlated well with resistance to beta-lactamase hydrolysis except against pseudomonas aeruginosa. hr 756, cefoxitin, and cefuroxime inhibited type i beta-lactamases, but not type iii, iv, or v enzyme ... | 1978 | 101128 |
| effect of different lots of mueller-hinton agar on the interpretation of the gentamicin susceptibility of pseudomonas aeruginosa. | population distributions and quality control data for strains of pseudomonas aeruginosa tested for gentamicin susceptibility on six lots of mueller-hinton agar were analyzed. the lots of agar were used in three university of washington hospitals from april 1975 through october 1977. the analyses indicated that the performance of members of the p. aeruginosa populations in each hospital closely followed the performance of the quality control strain, p. aeruginosa atcc 27853, when tested on each l ... | 1978 | 101130 |
| analysis of the interactions between piperacillin, ticarcillin, or carbenicillin and aminoglycoside antibiotics. | enhanced activity against clinical isolates of pseudomonas aeruginosa was demonstrated with piperacillin, ticarcillin, and carbenicillin in combination with gentamicin, tobramycin, and amikacin. | 1978 | 101133 |
| proposed ticarcillin disk control values for escherichia coli and pseudomonas aeruginosa: multicenter cooperative study. | in a multicenter cooperative controlled study, individual test, accuracy, and precision control values were determined for 75-mug ticarcillin disks with escherichia coli atcc 25922 and pseudomonas aeruginosa atcc 27853. | 1978 | 101134 |
| in vitro comparison of kanamycin, kanendomycin, gentamicin, amikacin, sisomicin, and dibekacin against 200 strains of pseudomonas aeruginosa. | the antimicrobial activity of kanamycin, kanendomycin, gentamicin, amikacin, sisomicin, and dibekacin against 200 strains of pseudomonas aeruginosa was compared. dibekacin was found to be the most active against the tested organisms, whereas the other aminoglycoside antibiotics fell in the following order of diminishing antibacterial potency: amikacin, sisomicin, gentamicin, kanendomycin, and kanamycin. seven strains showed high-level resistance to gentamicin (minimal inhibitory concentration, 4 ... | 1978 | 101135 |
| [distribution of pseudomonas aeruginosa in experimentally infected mice (author's transl)]. | the distribution of experimentally administered pseudomonas aeruginosa was studied in germfree cf no. 1 mice, barrier-sustained ddd mice with or without oral treatment of aminobenzyl-penicillin and conventional ddd mice. on day 1 after oral administration of 8 x 10(7) organisms, more than 10(8) of organisms/g were recovered from the feces of ex-germfree mice and the number was maintained during the experiment. on the other hand, from barrier-sustained mice with antibiotic treatment and those wit ... | 1978 | 101380 |
| [serotyping of pseudomonas aeruginosa]. | 1978 | 101427 | |
| pseudomonas ribosomal vaccines: preparation, properties, and immunogenicity. | the preparation, properties, and immunogenicity of ribosomal vaccines from pseudomonas aeruginosa are described. these preparations, containing protein and rna, were tested for immunogenicity by active immunization of mice and subsequent challenge with homologous, live bacteria. the results demonstrated that vaccines prepared from a majority of serotypes used were immunogenic, i.e., afforded 60 to 100% mouse protection against a challenge inoculum containing 8 to 50 50% lethal doses. in some cas ... | 1978 | 101464 |
| role of nonagglutinating antibody in the protracted immunity of vaccinated mice to pseudomonas aeruginosa infection. | effective immunization against infection with pseudomonas aeruginosa is difficult to evaluate because agglutinin levels decline rapidly. because fractionation of hyperimmune sera often yields more specific antibody than can be accounted for by direct agglutination tests, an immunoglobulin-specific assay based on antiglobulin augmentation was used to characterize antibody responses of c3h/hej mice vaccinated with p. aeruginosa type 2 lipopolysaccharide. nonagglutinating antibodies, initially dete ... | 1978 | 101467 |
| beta-lactam antibiotics. ii. structure-activity relationships of 6-[alpha-(alpha'-ureido-acylamino) acylamino] penicillanic acids. | the influence on the structure-activity relationships (s.a.r.) of the stereochemistry and various alkyl, aryl, aralkyl and heterocyclic substituents at the two chiral centres in the dipeptide side-chain of a new series of penicillins was examined. in many cases the effects of these changes had a pronounced influence on the degree of activity against gram-positive and especially gram-negative bacteria. several compounds indicated that the size, shape and spatial disposition of a substituent were ... | 1978 | 101499 |
| deletion plasmids from transformants of pseudomonas aeruginosa trp cells with the rsf1010-trp hybrid plasmid and high levels of enzyme activity from the gene on the plasmid. | a rsf1010-trp hybrid plasmid which contained the tryptophan operon of escherichia coli was introduced into pseudomonas aeruginosa trp cells by transformation. from the trp+ transformants several deletion plasmids were obtained, and their physical maps with restriction endonucleases were constructed. p. aeruginosa trp cells with these plasmids showed at first more than 100 times higher levels of tryptophan synthetase beta activity over that of the control p. aeruginosa wild-type cells, but these ... | 1978 | 101514 |
| outer membranes of gram-negative bacteria. xix. isolation from pseudomonas aeruginosa pao1 and use in reconstitution and definition of the permeability barrier. | a method for separating the outer and inner membranes of pseudomonas aeruginosa pao1 in the absence of added ethylenediaminetetraacetic acid was devised. the method yields two outer membrane fractions which show the same protein pattern on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but differ substantially in their relative contents of phospholipids. one of these outer membrane fractions and the inner membrane fraction are less than 4% cross-contaminated, as judged by the content ... | 1978 | 101518 |
| linkage map of pseudomonas aeruginosa pat. | the locations of new markers relative to markers previously mapped on the chromosome of pseudomonas aeruginosa strain pat were defined by generalized transduction with phage f116l and f1083. although the marker orders of the various marker groups were deduced mainly from the results of two-factor crosses, the locations of a number of markers were confirmed by three-factor crosses. a linkage map of the chromosome of p. aeruginosa pat was constructed which shows the relative locations of 50 genes. ... | 1978 | 101525 |
| relationship between catabolism of glycerol and metabolism of hexosephosphate derivatives by pseudomonas aeruginosa. | the relationship between catabolism of glycerol and metabolism of hexosephosphate derivatives in pseudomonas aeruginosa was studied by comparing the growth on glycerol and enzymatic constitution of strain pao with these characteristics of glucose-catabolic mutants and revertants. growth of strain pao on glycerol induced a catabolic oxidized nicotinamide adenine dinucleotide-linked glyceraldehyde-phosphate dehydrogenase and seven glucose-catabolic enzymes. the results indicated that these enzymes ... | 1978 | 101528 |
| a test for 'hygienic' hand disinfection. | a standardised test procedure is described in which finger-tips are inoculated with broth cultures of organisms (staphylococcus aureus, staphyloccocus saprophyticus, escherichia coli, and pseudomonas aeruginosa): counts are made from washings of hands after disinfection with various antiseptic-detergents, alcoholic solutions, or unmedicated soap. 70% alcohol, with or without chlorhexidine, was the most effective preparation. the two antiseptic detergents showed variable results, but against gram ... | 1978 | 101554 |
| effectiveness of immunization with single and multi-component vaccines prepared from a common antigen (oep), protease and elastase toxoids of pseudomonas aeruginosa on protection against hemorrhagic pneumonia in mink due to p. aeruginosa. | toxoids of protease and elastase of pseudomonas aeruginosa were successfully prepared by treatment with 8% formalin plus 0.2 m lysine and by 4% formalin respectively. the two toxoids proved sufficiently potent to elicit high antibody titers as estimated by both the enzyme-neutralizing and passive hemagglutination tests. the effectiveness of immunizing minks with a single component-vaccine consisting of the common antigen (oep) of p. aeruginosa or the protease toxoid (pt) or the elastase toxoid ( ... | 1978 | 101698 |
| studies on the detoxification of pseudomonas aeruginosa exotoxin. | 1978 | 101699 | |
| l-form of pseudomonas aeruginosa. 1. an effective method for the production of stable l-forms. | 1978 | 101700 | |
| the role of oep-antibodies in pseudomonas aeruginosa infection. | when mice were challenged intraperitoneally with 2.2 x 10(4) pseudomonas aeruginosa in 0.5 ml of 2.7% mucin solution, the bacteria markedly increased in the peritoneal cavity, rapidly entered the blood stream, and the mice died, probably of bacteremia, within a short time. however, when mice were injected subcutaneously 4 hours prior to the challenge with 0.2 ml of 0.75% igg (oep-ha = 12.8) or igm (oep-ha = 64) separated from human plasma or 3% s-iga ep-ha = 19.2) from fresh human milk, the bact ... | 1978 | 101701 |
| l-form of pseudomonas aeruginosa. 2. antibiotic sensitivity of l-forms and their parent forms. | 1978 | 101702 | |
| effect of certain sulphydryl compounds and dimethyl sulphoxide on potassium release by certain amines and proteins from pseudomonas aeruginosa. | dithiothreitol, cysteine, and mercapto-ethanol inhibit k+ release from cells of pseudomonas aeruginosa by amines and nh4cl incubated at ph 8.5. the inhibitions are competitive for the monamines and nh4cl but non-competitive for the diamines and spermidine. the percent inhibition is largest for tryptamine, least for spermidine and ethylene diamine. dmso inhibits k+ release by monamines and certain proteins but not significantly by diamines and histamine. the inhibitions are competitive. | 1977 | 101739 |
| [natural immunity. phagocytosis of pseudomonas aeruginosa in guinea pigs determined in vitro]. | 1978 | 102011 | |
| [lysine biosynthesis in pseudomonas aeruginosa pao 1. ii. first studies of dap decarboxylase of lysine auxotrophic mutant of p. aeruginosa pao 1]. | 1978 | 102088 | |
| comparison of the in vitro activity of bay k 4999 and piperacillin, two new antipseudomonal broad-spectrum penicillins, with other beta-lactam drugs. | bay k 4999 and piperacillin, two new substituted ampicillins, were compared with other beta-lactam antibiotics, including carbenicillin, azlocillin, mezlocillin, benzylpenicillin, ampicillin, and cefoxitin, against a wide range of gram-positive and -negative organisms. bay k 4999 and piperacillin were extremely active against pseudomonas aeruginosa (50% inhibited by 2 mug/ml), being about 16-fold more active than carbenicillin. bay k 4999 was the most active drug against escherichia coli (50% in ... | 1978 | 102245 |
| quantitative assessment of bactericidal activities of beta-lactam antibiotics by agar plate method. | quantitative bactericidal activities of beta-lactam antibiotics were determined by the agar plate method. broth cultures, of which the colony-forming units were counted before the study, were inoculated on antibiotic-containing agar plates, utilizing a 10(-3), 10(-2), or 10(-1) dilution or undiluted culture plated with each 0.001-ml calibrated loop. these plates were incubated at 37 degrees c overnight, and the minimal drug concentration at which no bacterial growth was observed on the plates wa ... | 1978 | 102246 |
| new antipseudomonal penicillin, pc-904: affinity to penicillin-binding proteins and inhibition of the enzyme cross-linking peptidoglycan. | the mechanism of action of a new antipseudomonal penicillin, pc-904, was studied with respect to its binding affinities to penicillin-binding proteins (pbps) and its inhibitory activities on cross-linking enzymes of peptidoglycan synthesis in vitro. pc-904 showed especially high affinity (compared with that of penicillin g) to escherichia coli pbp-3. it also had high affinities to pbp-2 and -1bs and low affinities to pbp-1a, -4, -5, and -6. similar results were obtained with pseudomonas aerugino ... | 1978 | 102247 |
| [pseudomonas aeruginosa epidemiology in egypt: study of 80 hospital strains isolated at alexandria]. | eighty hospital strains of p. aeruginosa, isolated in alexandria, were examined with the aid of three epidemiological markers (serotype, lysotype and o. n. p. g.-test). the most frequent serotypes in alexandria are 0:11, 0:12, 0:2, 0:4, 0:6 et 0:1; this situation is different from those reported in european countries and in u. s. a. sixty-five lysotypes were found and the strains of serotype 0:11 fall in fifteen lysotypes. one strain 0:12 was found o. n. p. g. positive. | 1978 | 102436 |
| in vitro activity of hr 756, a new cephalosporin compound. | the in vitro activity of hr 756, a new cephalosporin, has been determined against recent clinical isolates and compared with that of other beta-lactam antibiotics. the geometric means of the minimum inhibitory concentrations (mic) for different isolates of escherichia coli (100 isolates), klebsiella pneumoniae (84), pseudomonas aeruginosa (121), proteus mirabilis (52), indole-positive proteus species (9), salmonella species (19), staphylococcus aureus penicillin-sensitive (29) and penicillin-res ... | 1978 | 102631 |
| an explanation for the apparent host specificity of pseudomonas plasmid r91 expression. | pseudomonas aeruginosa strain 9169 has been reported to contain a plasmid that expresses resistance to carbenicillin (cb), kanamycin (km), and tetracycline (tc) in escherichia coli but resistance only to cb in certain pseudomonas recipients. the triply resistant plasmid in e. coli belonged to incompatibility (inc) group p or p-1, whereas the singly resistant plasmid in p. aeruginosa was compatible with incp-1 plasmids and other plasmids of established inc specificity but incompatible with plasmi ... | 1978 | 102634 |
| comparative toxicities of diphtherial toxin and pseudomonas aeruginosa exotoxin a: evidence for different cell receptors. | 1978 | 102734 | |
| provocation of pseudomoniasis with cyclophosphamide in mice. | after treatment with cyclophosphamide, a fatal syndrome was found in mice contaminated with pseudomonas aeruginosa. the syndrome seems to be caused by p. aeruginosa from the intestine, and the liver is suspected to be the most important target organ. | 1978 | 102866 |
| [a study of serotypes of pseudomonas aeruginosa isolated in man in saigon (vietnam) (author's transl)]. | the authors study the serological distribution of 143 strains of pseudomonas aeruginosa isolated in hospital grall (saigon-vietnam) according to the country (south vietnam), the kind of pathological specimens and the various departments of the hospial. they describe a special distribution of serological groups in vietnam with a large predominance of groups 0 = 6 and 0 = 11. they consider the possibility of adapting a polyvalent vaccine including the 10 serological groups now prevailing in vietna ... | 1978 | 102901 |
| [hospital infections with pseudomonas aeruginosa. ii. intensive care units (author's transl)]. | 72 newly admitted patients of three surgical intensive care units of the medical school hannover were examined bacteriologically for pseudomonas aeruginosa for a period of 7 months. a total of 810 specimens was examined during therapy. 95 strains of pseudomonas aeruginosa were isolated in 32 of 72 patients, taken either at the time of admission or during the stay of the patients in the hospital. the frequency of contamination increased with the duration of the stay of the patients in the hospita ... | 1978 | 102910 |
| [antibody response to somatic and flagellar antigens of pseudomonas aeruginosa (author's transl)]. | using the indirect fluorescent antibody technique, the production of antiflagellar and antisomatic antibodies in rabbits immunized with different p. aeruginosa strains is measured. after 6 injections of formalinized cultures in graded doses over 3 weeks, the antiflagellar antibodies reach the peak in the 4th to 5th week, followed by a rapid fall. the antisomatic antibodies reacting with native and boiled cells show a plateau-shaped maximum from the 3rd to 5th/6th week and fell slightly up to the ... | 1978 | 102912 |
| persistent bacteriuria in the rat after reflux challenge with pseudomonas aeruginosa. | an experiment based upon reflux of culture suspensions of pseudomonas aeruginosa into the rat kidney was set up to examine possible factors of importance in the development of ascending renal infection. we monitored the persistence of challenge bacteria in the rat urinary tract by quantitative culture of urine collected by spontaneous micturition, despite heavy contamination with commensal microflora. in most animals challenged, pseudomonads gave rise to bacteriuria of only limited duration. dur ... | 1978 | 103062 |
| renal infection in the rat after reflux challenge with pseudomonas aeruginosa. | a rat-pseudomonad reflux model was employed to examine the effect of the size of inoculum on the emergence of renal infection. widely varying degrees of renal disease observed in challenged animals indicated that a number of variables influenced the outcome of the host-parasite interaction. in this regard, animals in which there was a reduction of the colony counts below a threshold figure rarely gave evidence of renal infection when examined at autopsy on the eighth day following reflux. these ... | 1978 | 103063 |
| recurrent pseudomonas infection associated with neutrophil dysfunction. | a 72-year-old male is described with a history of 4 episodes of pseudomonas aeruginosa sepsis and chronic otitis media caused by pseudomonas species. in vitro testing of the patient's polymorphonuclear leukocytes (pmns) revealed profoundly abnormal chemotactic responses and defective intracellular killing of ps. aeruginosa, staphylococcus aureus and escherichia coli. chemiluminescence production by the patient's pmns in response to opsonized zymosan as well as endotoxin stimulated nitroblue tetr ... | 1978 | 103188 |
| [susceptibility of pseudomonas aeruginosa in relation to the methods of investigation]. | 1978 | 103321 | |
| [research in the stability of pyocin-types and subtypes in pseudomonas aeruginosa]. | 1978 | 103322 | |
| effect of two cancer chemotherapeutic agents on the antibacterial activity of three antimicrobial agents. | cancer chemotherapeutic agents and antibacterial antibiotics are often given concomitantly. daunorubicin, cytosine arabinoside, and three antibiotics (gentamicin, amikacin, and ticarcillin) were tested individually and in combinations to determine their antimicrobial activity against pseudomonas aeruginosa, klebsiella pneumoniae, and escherichia coli. these cytotoxic agents are commonly employed in the therapy of acute nonlymphocytic leukemia for remission induction therapy, and these antimicrob ... | 1978 | 103494 |
| frequency of f116-mediated transduction of pseudomonas aeruginosa in a freshwater environment. | transduction of pseudomonas aeruginosa streptomycin resistance by a generalized transducing phage, f116, was shown to occur during a 10-day incubation in a flow-through environmental test chamber suspended in a freshwater reservoir. mean f116 transduction frequencies ranged from 1.4 x 10(-5) to 8.3 x 10(-2) transductants per recipient during the in situ incubation. these transduction frequencies were comparable to transduction frequencies determined in preliminary laboratory transduction experim ... | 1978 | 103503 |
| a comparison of media used for the early detection of the bluish-green pigment of pseudomonas aeruginosa. | 1978 | 103694 | |
| experimental urinary tract infection with pseudomonas aeruginosa in mice. | urinary tract infection with pseudomonas aeruginosa was induced in mice by transurethral inoculation of the organism into the bladder, followed by urethral obstruction for 6 h. the infection was mostly localized in the urinary organs. p. aeruginosa p9 was selected as the challenge organism from 10 laboratory strains of p. aeruginosa. after the inoculation of 10(7) colony-forming units of p. aeruginosa p9, transient bacteremia was observed in some of the mice from 6 h to 1 day after the inoculati ... | 1978 | 103833 |
| r-factor inheritance and plasmid content in mucoid pseudomonas aeruginosa. | eighteen strains of alginate-producing mucoid pseudomonas aeruginosa were evaluated with respect to plasmid content and the ability to maintain well-characterized r plasmids. the spontaneous loss of alginate production in these strains varied from 0.01 to 0.7% and was not significantly increased by plasmid curing regimens. examination of cleared lysates of these strains and their isogenic nonmucoid derivatives by agarose gel electrophoresis failed to reveal plasmid dna. r-plasmid (p-incompatibil ... | 1978 | 103834 |
| biological activities of pyochelins: iron-chelating agents of pseudomonas aeruginosa. | strains of pseudomonas aeruginosa able to grow readily in serum (serum resistant) produce siderophores in large quantity, enabling them to extract iron from transferrins. the term pyochelin has been proposed for this group of compounds. pyochelin extractable with ethyl acetate and designated pyochelin a appears to be a mixture of catechols and other phenolates. the structures of water-soluble siderophores, designated pyochelin b, have not been determined. pyochelins enabled growth in serum of st ... | 1978 | 103839 |
| isolation and characterization of a high-molecular-weight polysaccharide from the slime of pseudomonas aeruginosa. | a procedure is described for isolating a high-molecular-weight polysaccharide (ps) from the slime of pseudomonas aeruginosa immunotype 1. the resultant material, obtained from the void volume of a sephadex g-100 column, was composed of carbohydrate and water. no lipopolysaccharide (lps), 2-keto-3-deoxyoctonoate, heptose, phosphate, or protein was detectable, and nucleic acid contamination was generally below 1%. the carbohydrate composition of the ps was glucose, rhamnose, galactose, arabinose, ... | 1978 | 103840 |
| protective immunity induced in mice by immunization with high-molecular-weight polysaccharide from pseudomonas aeruginosa. | a high-molecular-weight alkali-labile polysaccharide (ps) isolated from the slime of immunotype 1 pseudomonas aeruginosa was tested for its ability to protect mice from lethal challenge with the live, homologous organism. intraperitoneal (i.p.) injection of 10 to 25 microgram of the ps protected 60 to 70% of the mice against challenge with up to 50 50% lethal dose units. although single immunization of mice with up to 250 microgram of ps effected protective levels of only 70%, two successive imm ... | 1978 | 103841 |
| [efficacy, tolerance, and pharmacokinetics of sisomicin in urinary tract infections (author's transl)]. | since 1973 30 patients with urinary tract infections (uti) or pyelonephritis have been treated with sisomicin, a new aminoglycoside, in a daily dose of 2 mg/kg for a period of seven to nine days. from a clinical point of view the result of treatment was good. complete resolution was achieved in 17 patients, improvement in nine, and there was no effect in four patients. thirty-five causative organisms (escherichia coli = 23, proteus sp. = 7, klebsiella sp. = 3, pseudomonas aeruginosa = 1, citroba ... | 1978 | 103844 |
| type-specific indirect hemagglutinating antibody in patients with pseudomonas aeruginosa infection. | lipopolysaccharide antigens were extracted from heated cell extracts of several serotypes of pseudonomas aeruginosa. indirect hemagglutination with the extracts indicated specific reactivity with sera from rabbits immunized with homologous serotypes of p. aeruginosa. sera from healthy adults and patients infected with p. aeruginosa were studied subsequently and shown to possess antibodies against p. aeurginosa. in patients infected with p. aeruginosa type e, indirect hemagglutination antibody ag ... | 1978 | 103887 |
| regulation of enzyme synthesis in the arginine biosynthetic pathway of pseudomonas aeruginosa. | in pseudomonas aeruginosa the synthesis of only two out of eight arginine biosynthetic enzymes tested was regulated. comparisons were made between the specific activities of these enzymes in bacteria grown on arginine or on its precursor, glutamate. n2-acetylornithine 5-aminotransferase (acoat), an enzyme involved in both the biosynthesis and catabolism of arginine, was induced about 14-fold during growth of the organism on arginine as the only carbon and nitrogen source, and the anabolic ornith ... | 1978 | 103997 |
| the chemical composition of the lipopolysaccharide from pseudomonas aeruginosa strain pao and a spontaneously derived rough mutant. | the chemical composition of the lipopolysaccharide (lps) of the smooth strain pseudomonas aeruginosa pao 307 and a spontaneously derived rough mutant, obtained by selection for resistance to the lps-specific phage e79, are compared. the rough lps was shown to contain lipid a, heptose, 2-keto 3-deoxyoctonic acid, galactosamine, alanine and phosphate but lacked glucose, rhamnose and fucosamine which were important constituents, on a weight basis, of the smooth lps. these results, and chromatograph ... | 1977 | 104126 |
| relationship between mutant amidases of pseudomonas aeruginosa and hydroxyurea as an inhibitor. | hydroxyurea inhibited growth of pseudomonas aeruginosa strain ai 3 on media containing either acetanilide (n-phenyl acetamide) or acetamide as sole carbon sources. mutants resistant to hydroxyurea inhibition of growth on acetanilide (ouch strains) and acetamide (amouch strains) displayed altered growth properties on various amide media compared with the parent strain ai3. ai3 amidase, which catalyses the initial step in the metabolism of acetanilide and acetamide, was inhibited by hydroxyurea in ... | 1978 | 104140 |
| chromosomal location of genes participating in the degradation of purines in pseudomonas aeruginosa. | genetic mapping of the genes (puu) that encode the enzymes catalysing degradation of purines in pseudomonas aeruginosa strain pao has been carried out. mutants that are deficient in adenine deaminase (puua), guanine deaminase (puub), xanthine dehydrogenase (puuc), uricase (puud), allantoinase (puue), and/or allantoicase (puuf) were isolated and used for the genetic study. conjugation by fp5 factor and generalized transduction by phage g101 gave the following map locations of these six genes on t ... | 1978 | 104142 |
| hemolysin of myxococcus fulvus nk 35 i. production and isolation. | myxococcus fulvus nk 35 has been shown to produce a soluble hemolysin which lysed rabbit, human, horse, and sheep erythrocytes. a medium (varghese's medium) was devised in which a maximum of hemolysin was produced in 6 days at 28 degrees c under static conditions. the lysin was precipitated by complete saturation of the culture filtrate with ammonium sulphate, followed by dialysis against saline. other enzyme systems were destroyed by heating at 100 degrees c. further purification was achieved b ... | 1978 | 104473 |
| [flagella specific h antigenic schema of pseudomonas aeruginosa (author's transl)]. | serogrouping of p. aeruginosa on the basis of heat-stable somatic (o-)agglutinogens is well established, but h-typing on the basis of heat-labile agglutinogens is not accepted generally because of uncertain flagella specificity. in contrast to the agglutination reaction, the indirect fluorescent antibody technique allows the differentiation of soma and flagellum of p. aeruginosa cells at the morphological and serological level simultaneously, and thereby the specific analysis of flagellar (h-)an ... | 1978 | 104481 |
| [l-forms and pigment production in "pseudomonas aeruginosa" (author's transl)]. | 1978 | 104666 | |
| [microbiological studies on bacterial spectra of milk samples from healthy udder quarters and from those with increased cell counts and/or conductivity values]. | the germ levels of 2,182 milk samples obtained from udder quarters with subclinical mastitis were compared to milk sampled from 2,061 udder quarters with physiological cell counts or conductivity values. three cattle herds were involved in the test programme. no germ growth was established from 9.5 per cent of all samples taken from udder quarters with increased cell counts and conductivities and from 4.1 per cent of those samples taken from intact udder quarters. samples taken from udder quarte ... | 1978 | 104694 |
| pulmonary function and morbidity in 40 adult patients with cystic fibrosis. | pulmonary function and cardiopulmonary complications were studied in a group of 40 patients with cystic fibrosis who reached the age of 25 years. mean values for vital capacity (vc), functional residual capacity, residual volume (rv), the ratio of rv over total lung capacity (rv/tlc), conductance, and the ratio of the forced expiratory volume in one second over vc were abnormal. there was a variable pattern of progression from patient to patient. the men differed from the women only in that they ... | 1978 | 104832 |
| effectiveness of gentamicin and carbenicillin in a rat model against infections with pseudomonas aeruginosa resistant to gentamicin or gentamicin and carbenicillin. | 1979 | 104952 | |
| chemotaxis by pseudomonas aeruginosa. | chemotaxis by pseudomonas aeruginosa rm46 has been studied, and conditions required for chemotaxis have been defined, by using the adler capillary assay technique. several amino acids, organic acids, and glucose were shown to be attractants of varying effectiveness for this organism. ethylenediaminetetraacetic acid was absolutely required for chemotaxis, and magnesium was also necessary for a maximum response. serine taxis was greatest when the chemotaxis medium contained 1.5 x 10(-5) m ethylene ... | 1979 | 104961 |
| isolation of an iron-binding compound from pseudomonas aeruginosa. | an iron-binding compound was isolated from ethyl acetate extracts of culture supernatant fluids of pseudomonas aeruginosa and was purified by successive paper and thin-layer chromatographic procedures. the purified compound was characterized by uv, visible, infrared, and fluorescence spectroscopy. the compound possesses phenolic characteristics, with little or no similarity to dihydroxybenzoates and no indication of a hydroxamate group. p. aeruginosa synthesized the compound during active growth ... | 1979 | 104968 |
| homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria. | the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ... | 1979 | 104972 |
| effect of pyocin r1 on the glucose metabolism of sensitive cells of pseudomonas aeruginosa. | the effect of pyocin r1 on the glucose metabolism of sensitive pseudomonas cells was investigated. upon treatment with pyocin r1, although the rate of o2 uptake of the sensitive cells for glucose or gluconate was not very much affected at first, the final level of o2 uptake was greatly reduced. when 2-oxogluconate was used as a substrate, o2 uptake was immediately halted by pyocin. by determining the amounts of glucose, gluconate, and 2-oxogluconate before and after the reaction and the amount o ... | 1978 | 104980 |
| isolation of characterization of a major outer membrane protein of pseudomonas aeruginosa. evidence for the occurrence of a lipoprotein. | in the outer membrane of p. aeruginosa, a protein of apparent molecular weight 8,000 (protein i) is present as a major protein. purification and chemical analysis of protein i were carried out. this protein was purified by essentially the same procedure as for the purification of the e. coli lipoprotein, which was developed by inouye et al. (j. bacteriol. (1976) 127, 555--563). the amino acid composition of protein i was determined. protein i lacks proline, valine, isoleucine, phenylalanine, try ... | 1979 | 104984 |
| biochemical properties of a new flexuous bacteriocin, pyocin f1, produced by pseudomonas aeruginosa. | pseudomonas aeruginosa strain p15 produces not only pyocin r1 and phage ps10, but also a substance having a flexuous rod structure, the nature of which is so far unknown. a variant strain (p15--40) was obtained which produced these flexuous particles more effectively than the original strain, and the particles were purified to homogeneity and investigated. several strains of p. aeruginosa were found to be killed by the particles. it was concluded that the flexuous rod-like particles are not rela ... | 1979 | 104991 |
| complement-mediated phagocytosis of pseudomonas aeruginosa. | the nature of the opsonic factors in nonimmune human serum for six blood culture isolates of pseudomonas aeruginosa was investigated by measuring uptake of [3h] adenine-labeled bacteria by human pmns. normal human serum, c2- and c4-deficient sera, zymosan-treated serum, and immunoglobulin-deficient sera were used as opsonic sources. heat inactivation of each of these serum sources markedly reduced its opsonic capacity for all pseudomonas strains, suggesting that the serum c system was essential ... | 1978 | 105067 |
| transient defect in the bactericidal capacity of rabbit alveolar macrophages following sublethal shock. | the phagocytic function of alveolar macrophages obtained from rabbits after they were given sublethal shock by tumbling in a modified noble-collip drum was studied. as compared to the phagocytic function of alveolar macrophages from normal rabbits, tumbling produced a statistically significant reduction in in vitro bactericidal capacity for both pseudomonas aeruginosa and escherichia coli. however, there was no alteration in the ingestive phase. the reduced bactericidal capacity was evident with ... | 1979 | 105150 |
| pseudomonas sternotomy wound infection and sternal osteomyelitis. complications after open heart surgery. | pseudomonas aeruginosa sternotomy wound infections occurred in five patients who underwent open heart surgery. the initial isolate in each case was from a mediastinal chest tube routinely cultured on removal. soft-tissue infection developed in two patients, and sternal osteomyelitis developed in three patients. pseudomonas-typing studies showed a correlation between five isolates from chest-tube suction pumps used postoperatively and the wound isolates. analysis of antibiograms of pseudomonas wo ... | 1979 | 105156 |
| [colonization of pseudomonas aeruginosa in the digestive tract of limited-flora mice (author's transl)]. | 1978 | 105173 | |
| [serotype characteristics of clinical strains of pseudomonas aeruginosa]. | a total of 348 p. aeroginosa strains isolated from patients with pulmonary and pleural diseases were studied, and 87% of the test showed the possibility of their serotyping with the use of group-specific agglutinating antisera. serogroups ii, iii, iv were found to be prevalent among the strains isolated from patients with bronchopulmonary pathological states. correlations between definite groups of p. aeroginosa in their sensitivity to antibiotics were established; thus, the cultures belonging t ... | 1979 | 105521 |
| [experience with epidemiologic studies of pyocyaneus infections. i. feasibility of using serotypes and antibiotic resistance as a epidemiologic label for clinical strains]. | the authors carried out serological typing of 98 pseudomonas aeruginosa strains, isolated from patients of burn department of the sklifosovsky first aid institute in january-july, 1974, and of 215 strains obtained from other sources; their sensitivity to 13 antibiotics was determined. pseudomonas aeruginosa cultures isolated from the patients were typed with o-sera of 10 serological types. the presence of several hospital strains of pseudomonas aeruginosa was found by means of serological typing ... | 1978 | 105523 |
| [morphogenesis of pseudomonas aeruginosa colonies]. | 1978 | 105746 | |
| therapeutic experience with netilmicin. | netilmicin, a new aminoglycoside antibiotic, was used to treat 19 patients with urinary tract infection and 5 with systemic infection. the causal organisms were escherichia coli (in 2), klebsiella pneumoniae (in 4), serratia marcescens (in 12) and pseudomonas aeruginosa (in 7); 1 patient was infected with two of these organisms. all the isolates of causal organisms except one of serratia were initially sensitive to netilmicin but many were resistant to other aminoglycosides. sixteen of the urina ... | 1978 | 105797 |
| [evidence for plasmidic localization of "beta-lactamase" gene in a pseudomonas aeruginosa strain, highly resistant to carbenicillin]. | pseudomonas aeruginosa strain hl, isolated in besançon (france), shows a high level of resistance for carbenicillin, as proved in preliminary studies. in this paper, we report the transfer of carbenicillin resistance from pseudomonas aeruginosa hl1 carrying helper plasmids to other strains of the same species. comparative study of beta-lactamase activity in the hl1 strain and in the conjugated strains allows confirmation of the transfer of an r-factor from the hl1 strain: in all these strains, t ... | 1978 | 105822 |
| amikacin and netilmicin resistance in pseudomonas aeruginosa: incidence and transfer. | 1979 | 105839 | |
| microscopic characterization of rabbit lung damage produced by pseudomonas aeruginosa proteases. | the intratracheal administration of highly purified pseudomonas aeruginosa proteases (ca. 10 to 100 microgram) elicited extensive, grossly observable rabbit lung damage by 3 h postinjection. light and electron microscopic characterization of the lesions revealed: (i) progressive injury and necrosis of type i epithelial cells and capillary endothelial cells from 3 h to 1 day postinjection, and progressively increasing accumulations of erythrocytes, plasma proteins, fibrin, and released type ii e ... | 1979 | 106001 |
| phage-related surface modifications of pseudomonas aeruginosa: effects on the biological activity of viable cells. | lysogenic [ei(8)3] and phage 8-resistant mutant (ei/8s17) strains of pseudomonas aeruginosa ei were isolated. besides lacking the capacity to adsorb phage 8, strains ei(8)3 and ei/8s17 did not contain surface substrate for the depolymerase that is produced de novo when phage 8 infects wild-type strain. ei. the glycolipoprotein (glp) in the wild type contains phage 8 receptors and surface substrate for the depolymerase, as well as possesses characteristics of a virulence factor; therefore, the ch ... | 1979 | 106008 |
| defective cellular immunity to gram-negative bacteria in cystic fibrosis patients. | in vitro lymphocyte responses to pseudomonas aeruginosa have been found to be impaired in cystic fibrosis patients with advanced clinical disease. the responses to klebsiella pneumoniae, serratia marcescens, and proteus mirabilis were studied in a similar group of cystic fibrosis patients and normal individuals. cystic fibrosis patients found to be unresponsive to pseudomonas were also unresponsive to klebsiella, serratia, and proteus. responsiveness to staphylococcus aureus was not impaired in ... | 1979 | 106011 |
| passive immunization against pseudomonas with a ribosomal vaccine-induced immune serum and immunoglobulin fractions. | passive protection of mice against pseudomonas aeruginosa using specific antisera and immunoglobulin fractions induced by immunizing rabbits with a ribosomal vaccine is reported. the results demonstrated that protection by the ribosomal vaccine against challenge with live organisms can be serum mediated. previous work has shown that the vaccine can be separated into two components on the basis of molecular weight and that both higher (peak a)- and lower (peak b)-molecular-weight fractions were c ... | 1979 | 106013 |
| sensitivity of pseudomonas aeruginosa envelope mutants to alkylbenzyldimethylammonium chlorides. | a series of stepwise polymyxin-resistant envelope mutants of pseudomonas aeruginosa was used to test the activity of a homologous series (c10-c18) of alkylbenzyldimethylammonium chlorides. a sterilization kinetics procedure in deionized water was devised to avoid amounts quaternary compound above the critical micelle concentration. in all cases, there was a linear relationship between the logarithm of the rate of change of the colony count with time and the logarithm of the homolog concentration ... | 1979 | 106105 |
| [electrophoretic differences in the proteins of the species pseudomonas aeruginosa and pseudomonas denitrificans capable of oxidizing n-alkanes]. | pseudomonas aeruginosa and ps. denitrificans are capable of growing on n-alkanes, but differ in assimilation of higher and lower alkanes. the proteins of their cell-free homogenates were analysed by disc-electrophoresis in a system containing sds. the "preliminarily adapted" strain of ps. aeruginosa did not differ from the "non-adapted" to n-hexane strain, though both differed considerably from ps. denitrificans in the composition of protein spectrum. therefore, disc-electrophoresis of total pro ... | 1978 | 106218 |
| [isolation and general characteristics of a group of pseudomonas aeruginosa temperate phages]. | eighteen temperate phages were isolated from 38 natural strains of pseudomonas aeruginosa. the phages belong to five heteroimmune groups according to the ability of lysogenic variants of the strains pao1 and pat2 to exclude the growth of the superinfecting phage. phages of the ii and v immunity groups are serologically similar; the remaining phages are not inactivated by antisera against phages b26 (ii group) and k1338 (v group). prophages of the ii, iv, and v groups are induced by uv for vegeta ... | 1979 | 106221 |
| antibacterial activity of nitroxoline and sulphamethizole alone and in combination in urinary tract infections. | in vitro studies on the antibacterial activity of nitroxoline and sulphamethizole, alone and in combination, were undertaken and minimal inhibitory concentrations (mics) determined on a range of urinary pathogens. eighty per cent of the strains tested were sensitive to less than or equal to 16 mg/l of nitroxoline, and all strains, including pseudomonas aeruginosa and streptococcus faecalis, were sensitive to less than or equal to 64 mg/l of nitroxoline. no synergism could be demonstrated with su ... | 1978 | 106479 |
| [antibody induced phase variation in pseudomonas aeruginosa (author's transl)]. | to extend the flagella specific h antigenic schema of p. aeruginosa (ansorg, 1978), investigations are carried out to reveal phase variation in this species as in salmonella or arizona. with conventional methods, i.e. examination of single colonies after plating the cultures on standard nutrient agar or the phase reversal techniques of craigie (1931) and gard (1938) by adding homologous anti-oh-serum of different amounts in semisolid agar medium, no change of the flagellar antigens of various p. ... | 1978 | 106580 |
| [use of the indirect hemagglutination reaction for detecting antibodies to pseudomonas pyocyanea in acute suppurative destructive pneumonia]. | no less than 4-fold increase in the antibody titres to pseudomonas aeruginosa during the infectious process served as laboratory confirmation of its participation in the infectious process. in 49 of 91 patients hemagglutining titre exceeded the diagnostic one (1:640). in 9 patients (chiefly in infants) hemagglutinin titre remained low, but there was a rise of antibody level to pseudomonas aeruginosa during the disease. high hemagglutinin titres were noted in 12 patients on admission to the clini ... | 1978 | 106610 |
| [antibiotic resistance study of clinical strains of pseudomonas aeruginosa]. | the study of 40 clinical strains of ps. aeruginosa isolated from the wound surfaces of the patients showed that all the isolates were resistant to one or several antibiotics. the number of the strains resistant to 5, 4, 3, 2 or 1 drug was 5, 22.5, 25. 30 or 17.5 per cent respectively. fifteen strains carried resistance plasmids capable of conjugative transfer. eleven out of 21 plasmids controlled resistance to chloramphenicol, 7 plasmids controlled resistance to streptomycin and sulfanylamides, ... | 1979 | 106769 |