Publications

detailed structural analysis of a novel, specific o-linked glycan from the prokaryote flavobacterium meningosepticum.	in the preceding paper, preliminary analysis revealed a new type of o-linked oligosaccharide of 1244 da at each of two proposed glycosylation sites on several proteins secreted by the gram-negative bacterium flavobacterium meningosepticum (plummer, t. h., jr., tarentino, a. l., and hauer, c. r. (1995) j. biol. chem. 270, 13192-13196). in this report we detail the linkage, sequence, and branching of this unusual heptasaccharide by electrospray (es) ionization mass spectrometry (ms), and collision ...	1995	7768917
molecular cloning and sequence analysis of flavastacin: an o-glycosylated prokaryotic zinc metalloendopeptidase.	a new zinc metalloendopeptidase that cleaves peptides on the amino-terminal side of aspartic acid was isolated from the cultural filtrate of flavobacterium meningosepticum. the gene for this new enzyme was cloned into pbluescript, and the complete nucleotide sequence was determined. over 40% of the deduced amino acid sequence was verified independently by direct protein microsequencing. the most important structural features of this new enzyme include (i) the presence of an unusual o-linked olig ...	1995	7771796
cloning, sequencing, and expression of the dipeptidyl peptidase iv gene from flavobacterium meningosepticum in escherichia coli.	the dipeptidyl peptidase iv (dp iv, ec 3.4.14.5) gene from flavobacterium meningosepticum was cloned by southern and colony hybridizations using probes amplified by pcr, and expressed in escherichia coli dh1. e. coli dh1 harboring pfdp-h1, which was a subclone derived from the positive clone pfdp-1, showed 3.5-fold higher activity than f. meningosepticum. nucleotide sequencing analysis revealed an open reading frame of 2133 bp, coding for a protein of 711 amino acids with a predicted molecular w ...	1995	7793970
molecular cloning and sequence analysis of flavobacterium meningosepticum glycosylasparaginase: a single gene encodes the alpha and beta subunits.	a full-length insert for the flavobacterium meningosepticum n4-(n-acetyl-beta-glucosaminyl)-l-asparagine amidase gene was located on a 2500-bp hindiii fragment and cloned into the plasmid vector pbluescript. dna sequencing revealed an open reading frame of 1020 nucleotides encoding a putative 45-amino-acid leader sequence and a deduced precursor polypeptide of 295 amino acids. in f. meningosepticum this precursor polypeptide undergoes proteolytic processing by an as yet unknown mechanism to gene ...	1995	7840643
the major n-linked carbohydrate chains from human urokinase. the occurrence of 4-o-sulfated, (alpha 2-6)-sialylated or (alpha 1-3)-fucosylated n-acetylgalactosamine(beta 1-4)-n-acetylglucosamine elements.	the primary structure of the major n-linked carbohydrate chains attached to asn302 of urinary-type plasminogen activator (urokinase) have been determined. urokinase was completely deglycosylated with peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f from flavobacterium meningosepticum. released oligosaccharides were separated from the remaining protein using gel-permeation chromatography on bio-gel p-100, and then on bio-gel p-6. fractionation of the oligosaccharides was achieved by a ...	1995	7737145
intrathecal immune response in neonatal flavobacterium meningosepticum meningitis.	neonates are predisposed to serious infections such as meningitis, probably due to their immature host reaction to the pathogens. we have studied the intrathecal immune response in 2 newborns with flavobacterium meningosepticum meningitis. they showed a significant elevation of immunoglobulin indices ( >1.10), also after the csf had become sterile with a normalized cell count. in addition, an intrathecal increase and subsequent decrease of both c3dg and tcc (terminal complement complex) were obs ...	1995	8534775
quinolone susceptibility of multiply-resistant flavobacterium meningosepticum clinical isolates in one urban hospital.		1995	8549290
successful combination vancomycin and rifampin therapy in a newborn with community-acquired flavobacterium meningosepticum neonatal meningitis.		1995	8584328
relapse of catheter-related flavobacterium meningosepticum bacteremia demonstrated by dna macrorestriction analysis.	a 6-year-old boy with non-hodgkin's lymphoma presented because of recurrent episodes (on 13 september 1993 and 12 january 1994) of possibly catheter-related bacteremia. the strains isolated during both episodes and seven epidemiologically unrelated control strains were typed by pulsed-field gel electrophoresis (pfge) of chromosomal dna. the similarity of the pfge patterns of the two isolates suggests that both episodes of bacteremia were caused by the same strain. the antimicrobial susceptibilit ...	1995	8645856
investigations on the mechanism of adipocere formation and its relation to other biochemical reactions.	in the adipocere, which is one of the postmortem changes, some specific fatty acids possessing higher melting points, together with soap, play an important role in the formation of adipocere. these fatty acids were shown to be mainly 10-hydroxystearic and 10-hydroxypalmitic acids. moreover, slight amounts of 10-oxostearic and 10-oxopalmitic acids, which have higher melting points than those of hydroxy fatty acids, exist in the adipocere as well. the substantial adipocere is formed and stabilized ...	1996	8690322
development of techniques to genetically manipulate members of the genera cytophaga, flavobacterium, flexibacter, and sporocytophaga.	the bacteroides-cytophaga-flavobacterium branch of the eubacterial phylogenetic tree contains a diverse group of bacterial species. techniques for the genetic manipulation of bacteroides spp. are well developed (a. a. salyers, n. b. shoemaker, and e. p. guthrie, crit. rev. microbiol. 14:49-71, 1987). recently we developed techniques to genetically manipulate the gliding bacterium cytophaga johnsonae (m. j. mcbride and m. j. kempf, j. bacteriol. 178:583-590, 1996). we now demonstrate that some of ...	1996	8702294
flavobacterium meningosepticum meningitis in an adult with acute leukaemia.		1996	8731719
[meningitis caused by flavobacterium meningosepticum in an adult].		1996	8756220
ribotyping of chryseobacterium meningosepticum: its use as an epidemiological tool and its correlation with serovars.	chryseobacterium meningosepticum (basonym, flavobacterium meningosepticum king 1959) is associated with neonatal meningitis and is isolated from normal and immunocompromised adults. aaf-labelled escherichia coli 16 + 23s rrna was used as a probe for ribotype analysis of 92 clinical isolates from tracheal exsudate, blood culture, cerebrospinal fluid, urine and pus. the 92 isolates belonged to the 15 described serovars of c. meningosepticum, and included 21 strains isolated during an outbreak in a ...	1996	8763627
porcine fibrinogen glycopeptides: substrates for detecting endo-beta-n-acetylglucosaminidases f2 and f3(1).	two different glycopeptides were isolated in high yield from a thermolytic digest of porcine fibrinogen. edman analysis established their sequences as val-glu-asn(cho)-lys and val-gly-glu-asn(cho)-arg. these sequences are nearly identical to the two human fibrinogen glycopeptides, val-glu-asn(cho)-lys (gamma-chain), and met-gly-glu-asn(cho)-arg (beta-chain). the predominant carbohydrate moiety of both asialoglycopeptides was a biantennary oligosaccharide with a core alpha(1-->6)-linked fucose as ...	1996	8850552
prolyl aminopeptidase gene from flavobacterium meningosepticum: cloning, purification of the expressed enzyme, and analysis of its sequence.	in spite of the numerous studies regarding prolyl aminopeptidase, little is known about its mechanism and the significance of its similarity to a number of hydrolases of diverse specificity that belong to the alpha/beta hydrolase-fold family (pseudomonas 2-hydroxymuconic semialdehyde hydrolase, atropinesterase, and 2-hydroxy-6-oxophenylhexa-2,4-dienoic acid hydrolase; human and rat epoxide hydrolases). we report the cloning and sequencing of the novel prolyl aminopeptidase gene from flavobacteri ...	1996	8951032
deglycosylation of proteins for crystallization using recombinant fusion protein glycosidases.	obtaining high quality protein crystals remains a rate-limiting step in the determination of three-dimensional x-ray structures. a frequently encountered problem in this respect is the high or heterogeneous carbohydrate content of many eukaryotic proteins. a number of reports have demonstrated the use of enzymatic deglycosylation in the crystallization of certain glycoproteins. although this is an attractive tool, there are some problems that hinder the more widespread use of glycosidases in cry ...	1996	8976570
flavobacterium meningosepticum: an unusual pathogen.		1996	8979561
dipeptidyl aminopeptidase iv from pseudomonas sp. wo24.	dipeptidyl aminopeptidase iv from pseudomonas sp. wo24 was purified as two molecular forms of 84 and 82-kda by sds-page. peptide mapping and n-terminal sequence analyses indicated that both proteins might be derived from the same protein, and that the 82-kda molecule might be a truncated form from the 84-kda molecule at least at the n-terminus. the dap iv gene of pseudomonas sp. wo24 was cloned and expressed in e. coli. the enzyme expressed in e. coli jm109 harboring a hybrid plasmid, pyo-6a, wi ...	1996	8988635
activation of glycosylasparaginase. formation of active n-terminal threonine by intramolecular autoproteolysis.	the activation mechanism of glycosylasparaginase of flavobacterium meningosepticum has been analyzed by site-directed mutagenesis and activation of purified precursors in vitro. mutation of thr-152 to ser or cys leads to gene products that are not activated in vivo but are activated in vitro because processing of the mutant precursors is inhibited by certain amino acids in the cell. kinetic studies reveal that activation is an intramolecular autoproteolytic process. the involvement of his-150 an ...	1996	8576176
cloning, sequencing, and expression of arthrobacter protophormiae endo-beta-n-acetylglucosaminidase in escherichia coli.	the gene encoding endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) was cloned, and its nucleotide sequence was determined. a single open reading frame consisting of 1935 base pairs and encoding a polypeptide composed of signal peptides of 24 amino acids and a mature protein of 621 amino acids was found. the primary structure of endo-a exhibited significant homology with fo1f.10 gene product from caenorhabditis elegans and weak homology with peptide-n4-(n-acetyl-beta-d-g ...	1997	9015383
a cytosolic serine endopeptidase from trypanosoma cruzi is required for the generation of ca2+ signaling in mammalian cells.	an early event in the trypanosoma cruzi cell invasion process, the recruitment of host lysosomes, led us to investigate the involvement of signal transduction. infective trypomastigotes were found to contain a soluble ca2+-signaling activity for mammalian cells that is sensitive to protease inhibitors. inhibitor and substrate utilization profiles were used to purify a candidate peptidase for involvement in this process, from which we isolated a full-length cdna clone. the sequence revealed a nov ...	1997	9024691
chryseobacterium meningosepticum: an emerging pathogen among immunocompromised adults. report of 6 cases and literature review.	chryseobacterium meningosepticum is a ubiquitous gram-negative bacillus historically associated with meningitis in premature neonates. we report 15 positive cultures and 6 cases of infection among immunocompromised adults at our institution over a 10-year period and review the english-language literature on c. meningosepticum. excluding the present series, there are 308 reports of positive cultures in the literature, of which 59% were determined to represent true infections. sixty-five percent o ...	1997	9064486
adjuvanticity of an ornithine-containing lipid of flavobacterium meningosepticum as a candidate vaccine adjuvant.	ornithine-containing lipids (ornl) extracted from flavobacterium meningosepticum have been reported to have various biological activities such as b-cell mitogenicity and macrophage activation to generate interleukin-1 and prostaglandin e2. we, using ovalbumin (ova) as an antigen, evaluated the adjuvant activity of ornl as an immunological adjuvant in balb/c mice. ornl showed the function of forming liposome-like vesicles retaining biological activities when prepared as either small unilamellar o ...	1997	9087952
specific uptake of tumor necrosis factor-alpha is involved in growth control of trypanosoma brucei.	trypanosoma brucei is lysed by tumor necrosis factor-alpha (tnf-alpha) in a dose-dependent way, involving specific binding of the cytokine to a trypanosomal glycoprotein present in the flagellar pocket of the parasite. tnf-alpha-gold particles are endocytosed via coated pits and vesicles and are directed towards lysosome-like digestive organelles. the specific uptake of the cytokine by the parasite results in a developmentally regulated loss of osmoregulatory capacity. tnf-alpha specific lysis i ...	1997	9151676
antimicrobial susceptibility of flavobacteria as determined by agar dilution and disk diffusion methods.	a total of 106 clinical isolates of flavobacteria, including 41 isolates of flavobacterium meningosepticum, 59 of flavobacterium indologenes, and 6 of flavobacterium odoratum were collected from january 1992 to december 1995 from patients in taiwan. the in vitro activities of antimicrobial agents were determined concomitantly by the standard agar dilution and disk diffusion methods. more than 90% of the flavobacterial isolates were resistant to cephalothin, cefotaxime, ceftriaxone, moxalactam, a ...	1997	9174188
lipohexin, a new inhibitor of prolyl endopeptidase from moeszia lindtneri (hki-0054) and paecilomyces sp. (hki-0055; hki-0096). ii. inhibitory activities and specificity.	the new proline-containing lipohexapeptide lipohexin (i) isolated from three fungal strains, moeszia lindtneri (hki-0054) and paecilomyces sp. (hki-0055 and hki-0096) is a competitive inhibitor of prolyl endopeptidase (pep) from human placenta with ic50 of 3.5 microm. specificity of lipohexin (i) is indicated by the much weaker inhibitory activity against bacterial prolyl endopeptidase from flavobacterium meningosepticum (ic50 25 microm). no effect of lipohexin (i) was found on the activity of m ...	1997	9207907
comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative rods.	in the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ...	1997	9210669
detailed studies on substrate structure requirements of glycoamidases a and f.	glycoamidases (peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase, ec 3.5.1.52; also known as peptide: n-glycanases (pngases) release n-linked oligosaccharides from glycopeptides and/or glycoproteins by hydrolyzing the glycosylated beta-amide bond of the asparagine side chain. the most widely used glycoamidases are those from flavobacterium meningosepticum (glycoamidase f or pngase f) and almond emulsin (glycoamidase a or pngase a). to study the substrate structure requirement of these en ...	1997	9341145
cloning and expression of the cdna encoding prolyl oligopeptidase (prolyl endopeptidase) from bovine brain.	prolyl oligopeptidase (ec 3.4.21.26, prolyl endopeptidase) cdna from bovine brain was cloned by pcr, and the amplified fragment was used as a probe to screen the cdna library from bovine brain. the obtained clone contained a 2.7 kb dna fragment with an open reading frame of 2130 nucleotides, and encoded a protein of 710 amino acids with a deduced molecular weight of 80640 da. the deduced amino acid sequence is 95, 94, 51 and 48% homologous to those of human t-cell, porcine brain, aeromonas hydro ...	1997	9353562
susceptibilities of chryseobacterium indologenes and chryseobacterium meningosepticum to cefepime and cefpirome.	in vitro activities of cefepime and cefpirome against 96 isolates of chryseobacterium indologenes and 21 of c. meningosepticum were determined by the agar dilution method. overall, cefepime was more active than cefpirome against c. indologenes (mic at which 50% of the isolates were inhibited [mic50] and mic90, 4 and 16 microg/ml, respectively, for cefepime and 8 and 128 microg/ml, respectively, for cefpirome). both agents had poor potency against c. meningosepticum (mic50 and mic90, 64 and >256 ...	1997	9399547
otogelin: a glycoprotein specific to the acellular membranes of the inner ear.	efforts to identify the specific components of the mammalian inner ear have been hampered by the small number of neuroepithelial cells and the variety of supporting cells. to circumvent these difficulties, we used a pcr-based subtractive method on cdna from 2-day-old mouse cochlea. a cdna encoding a predicted 2910-amino acid protein related to mucin has been isolated. several lines of evidence indicate, however, that this protein does not undergo the o-glycosylation characteristic to mucins. as ...	1997	9405633
reappraisal of the antimicrobial susceptibilities of chryseobacterium and flavobacterium species and methods for reliable susceptibility testing.	several flavobacterium species, comprising a heterogeneous group of gram-negative bacilli that are capable of causing opportunistic infections in humans, have recently been reclassified as chryseobacterium or myroides species. intrinsically resistant to a number of antibiotics, these organisms have been reported to be susceptible to vancomycin and certain other drugs that are normally active against gram-positive bacteria. by using the national committee for clinical laboratory standards (nccls) ...	1997	9420049
nucleoside oxidase, a hydrogen peroxide-forming oxidase, from flavobacterium meningosepticum.	a novel enzyme which catalyzes the oxidation of nucleosides to nucleoside-5(prm1)-carboxylic acids, forming hydrogen peroxide, was purified to homogeneity from flavobacterium meningosepticum t-2799. the enzyme has a molecular weight of about 500,000, and four nonidentical subunits (molecular weights of 81,000, 69,000, 33,000, and 16,000) were detected on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. on the basis of visible absorption spectra of the purified enzyme, the enzyme is con ...	1997	16535726
flavobacterium meningosepticum in intensive care units of a teaching hospital in riyadh, saudi arabia.		1997	17377442
flavobacterium meningosepticum in icus.		1998	17341928
comparative activities of clinafloxacin against gram-positive and -negative bacteria.	activities of clinafloxacin, ciprofloxacin, levofloxacin, sparfloxacin, trovafloxacin, piperacillin, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ceftazidime, and imipenem against 354 ciprofloxacin-susceptible and -intermediate-resistant organisms were tested by agar dilution. clinafloxacin yielded the lowest quinolone mics (< or = 0.5 microg/ml against ciprofloxacin-susceptible organisms and < or = 16.0 microg/ml against ciprofloxacin-intermediate-resistant organisms) compared to tho ...	1998	9593165
kinship and diversification of bacterial penicillin-binding proteins and beta-lactamases.		1998	9449253
use of ribotyping and hemolysin activity to identify highly virulent streptococcus suis type 2 isolates.	nineteen streptococcus suis type 2 isolates were evaluated for their virulence in pigs and mice. of these, seven were determined to be highly virulent in pigs on the basis of clinical sign scores and gross pathology and histopathology results. clinical sign scores correlated with gross pathology and histopathology scores at p equal to 0.004 and p equal to 0.009, respectively. the virulence of highly virulent isolates in pigs compared somewhat with virulence in mice, but the correlation was not s ...	1998	9431912
sequence analysis of the porphyromonas gingivalis dipeptidyl peptidase iv gene.	we previously constructed a porphyromonas gingivalis genomic library and isolated the 2.9 kb ecorv fragment which specified glycylprolyl dipeptidyl aminopeptidase (gpase). nucleotide sequencing of this fragment identified the single 2169 bp open reading frame which coded for a 723 amino acid protein. the amino acid sequencing of the nh2-terminal domain of the native and recombinant mature enzymes suggested that the protease possessed a 16 amino acid residue signal peptide. the calculated mass of ...	1998	9524216
crystal structure of glycosylasparaginase from flavobacterium meningosepticum.	the crystal structure of recombinant glycosylasparaginase from flavobacterium meningosepticum has been determined at 2.32 angstroms resolution. this enzyme is a glycoamidase that cleaves the link between the asparagine and the n-acetylglucosamine of n-linked oligosaccharides and plays a major role in the degradation of glycoproteins. the three-dimensional structure of the bacterial enzyme is very similar to that of the human enzyme, although it lacks the four disulfide bridges found in the human ...	1998	9541410
development of a direct in situ pcr method for detection of specific bacteria in natural environments.	we applied hnpp (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ pcr for the routine detection of specific bacterial cells at the single-cell level. pcr was performed on glass slides with digoxigenin-labeled dutp. the digoxigenin-labeled pcr products were detected with alkaline phosphatase-labeled antidigoxigenin antibody and hnpp which was combined with fast red tr. a bright red fluorescent signal was produced from conversion to hnp (dephosphorylated form) by alkaline p ...	1998	9546190
structure and expression of the flaa periplasmic flagellar protein of borrelia burgdorferi.	the spirochete which causes lyme disease, borrelia burgdorferi, has many features common to other spirochete species. outermost is a membrane sheath, and within this sheath are the cell cylinder and periplasmic flagella (pfs). the pfs are subterminally attached to the cell cylinder and overlap in the center of the cell. most descriptions of the b. burgdorferi flagellar filaments indicate that these organelles consist of only one flagellin protein (flab). in contrast, the pfs from other spirochet ...	1998	9573194
identification of acinetobacters on blood agar in presence of d-glucose by unique browning effect.	a positive phenotypic characteristic of glucose-oxidizing acinetobacters was demonstrated with blood agar containing d-glucose. glucose-oxidizing acinetobacter baumannii, acinetobacter genospecies 3, acinetobacter lwoffii, and acinetobacter genospecies 13 sensu tjernberg and ursing caused a unique brown discoloration of media supplemented with 5% blood (of horse, sheep, or human origin) and an aldose sugar (0.22 m d-glucose, d-galactose, d-mannose, d-xylose, or lactose). the browning effect was ...	1998	9574714
characterization and sequence of the chryseobacterium (flavobacterium) meningosepticum carbapenemase: a new molecular class b beta-lactamase showing a broad substrate profile.	the metallo-beta-lactamase produced by chryseobacterium (formerly flavobacterium) meningosepticum, which is the flavobacterial species of greatest clinical relevance, was purified and characterized. the enzyme, named blab, contains a polypeptide with an apparent mr of 26000, and has a pi of 8.5. it hydrolyses penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate, a mechanism-based inactivator of active-site serine beta-lactamases. the enzyme was inhibited by ...	1998	9576862
in vitro antibiotic synergy against flavobacterium meningosepticum: implications for therapeutic options.	flavobacterium meningosepticum is an unusual, highly resistant, gram-negative bacillus that is associated with neonatal meningitis and nursery outbreaks of meningitis. the optimal therapy for this infection is not known. we successfully treated three neonates with intravenous vancomycin and rifampin. we determined the in vitro activity of meropenem, ciprofloxacin, vancomycin, linezolid (pnu-100766), and rifampin, alone and in combination, against four isolates of f. meningosepticum from neonates ...	1998	9597247
cytoplasmic membrane lipoprotein lppc of streptococcus equisimilis functions as an acid phosphatase.	the function of the streptococcal cytoplasmic membrane lipoprotein, lppc, was identified with isogenic streptococcus equisimilis h46a and escherichia coli jm109 strain pairs differing in whether they contained [h46a and jm109(plpp2)] or lacked (h46a lppc::plpp10 and jm109) the functional lppc gene for comparative phosphatase determinations under acidic conditions. lppc-directed acid phosphatase activity was demonstrated zymographically and by specific enzymatic activity assays, with whole cells ...	1998	9647812
evaluation of the vitek 2 system for rapid identification of medically relevant gram-negative rods.	the new vitek 2 system (biomérieux) was evaluated at two independent sites with the identification card for gram-negative bacilli (id-gnb card). of the 845 strains tested, which represented 70 different taxa belonging to either the family enterobacteriaceae or the nonenteric bacilli, 716 (84.7%) were correctly identified at the species level. thirty-two (3.8%) additional strains were identified to the species level after the performance of simple, rapid manual tests (oxidase, hemolysis, indole r ...	1998	9650942
chemical structure of lipid a isolated from flavobacterium meningosepticum lipopolysaccharide.	the chemical structure of the lipid a of the lipopolysaccharide component isolated from flavobacterium meningosepticum ifo 12535 was elucidated. methylation and nuclear magnetic resonance analyses showed that two kinds of hydrophilic backbone exist in the free lipid a: a beta (1-->6)-linked 2-amino-2-deoxy-d-glucose, which is usually present in enterobacterial lipid a's, and a 2-amino-6-o-(2, 3-diamino-2,3-dideoxy-beta-d-glucopyranosyl)-2-deoxy-d-glucose, in a molar ratio of 1.00:0.35. both back ...	1998	9683486
crystal structures of flavobacterium glycosylasparaginase. an n-terminal nucleophile hydrolase activated by intramolecular proteolysis.	glycosylasparaginase (ga) is a member of a novel family of n-terminal nucleophile hydrolases that catalytically use an n-terminal residue as both a polarizing base and a nucleophile. these enzymes are activated from a single chain precursor by intramolecular autoproteolysis to yield the n-terminal nucleophile. a deficiency of ga results in the human genetic disorder known as aspartylglycosaminuria. in this study, we report the crystal structure of recombinant ga from flavobacterium meningoseptic ...	1998	9685368
a relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses.	adhesion of bacteria to hydrogel lenses is thought to be an initial step of ocular colonization allowing evasion of normal host defences. the salt concentration of media is an important parameter controlling microbial adhesion. salinity varies from 0.97% nacl equivalents in the open eye to 0.89% in the closed eye state. in this study, the effect of sodium chloride in the concentration range of 0.8-1.0% (w/v) nacl on adhesion of ocular bacteria to soft contact lenses was investigated using a stat ...	1998	9717279
reversible unfolding of intact and cleaved prolyl oligopeptidase from flavobacterium meningosepticum induced by urea.		1998	9723741
prolyl endopeptidase from sphingomonas capsulata: isolation and characterization of the enzyme and nucleotide sequence of the gene.	prolyl endopeptidase (prolyl oligopeptidase, ec 3.4.21.26) was purified from sphingomonas capsulata ifo 12533, and its gene was cloned and expressed in escherichia coli. the recombinant enzyme was markedly inhibited by diisopropyl phosphofluoridate and hardly affected by sh reagents or metal chelators, similar to the native enzyme purified from s. capsulata. nucleotide sequencing analysis revealed an open reading frame of 2169 bp, coding for a protein of 723 amino acids with a predicted molecula ...	1998	9750174
[evaluation of antibacterial activities of various antibiotics against glucose non-fermentative gram-negative rods other than pseudomonas aeruginosa].	mics of piperacillin, sulbactam/cefoperazone, minocycline (mino), gentamicin, amikacin, flomoxef, ceftazidime, cefozopran, cefsulodin and imipenem were determined, against 189 clinical isolated strains of glucose non-fermentative gram-negative rods (nfgnr; acinetobacter baumannii (44), alcaligenes faecalis (5), alcaligenes xylosoxidans (25), burkholderia cepacia (12), chryseobacterium indologenes (23), chryseobacterium meningosepticum (9), pseudomonas fluorescens (8), pseudomonas putida (12), st ...	1998	9755431
structure and glycosylation patterns of surface proteins from woodchuck hepatitis virus.	woodchucks chronically infected with woodchuck hepatitis virus (whv) are a valuable model for human hepatitis b virus (hbv) in studies of pathogenesis, immunity, and antiviral therapy. for this reason, substantial efforts to characterize both the similarities and the differences between hbv and whv are being made. the structure of the whv surface proteins (whs proteins) has not yet been adequately elucidated. the bands that would be expected for glycosylated and nonglycosylated small (s) whs pro ...	1998	9811735
paralemmin, a prenyl-palmitoyl-anchored phosphoprotein abundant in neurons and implicated in plasma membrane dynamics and cell process formation.	we report the identification and initial characterization of paralemmin, a putative new morphoregulatory protein associated with the plasma membrane. paralemmin is highly expressed in the brain but also less abundantly in many other tissues and cell types. cdnas from chicken, human, and mouse predict acidic proteins of 42 kd that display a pattern of sequence cassettes with high inter-species conservation separated by poorly conserved linker sequences. prenylation and palmitoylation of a cooh-te ...	1998	9813098
characterization of the prolyl dipeptidyl peptidase gene (dppiv) from the koji mold aspergillus oryzae.	the koji mold aspergillus oryzae secretes a prolyl dipeptidyl peptidase (dppiv) when the fungus is cultivated in a medium containing wheat gluten as the sole nitrogen and carbon source (mmwg). we cloned and sequenced the dppiv gene from an a. oryzae library by using the a. fumigatus dppiv gene as a probe. reverse transcriptase pcr experiments showed that the a. oryzae dppiv gene consists of two exons, the first of which is only 6 bp long. the gene encodes an 87.2-kda polypeptide chain which is s ...	1998	9835566
community-acquired chryseobacterium meningosepticum pneumonia and sepsis in a previously healthy child.		1998	9892546
a novel glycerol kinase from flavobacterium meningosepticum: characterization, gene cloning and primary structure.	a thermostable glycerol kinase (fgk) was purified 34-fold to homogeneity from flavobacterium meningosepticum. the molecular masses of the enzyme were 200 kda by gel filtration and 50 kda by sds-page. the km for glycerol and atp were 0.088 and 0.030 mm, respectively. the enzyme was stable at 65 degrees c for 10 min and at 37 degrees c for two weeks. the enzyme gene was cloned into escherichia coli and its complete dna was sequenced. the fgk gene consists of an open reading frame of 1494-bp encodi ...	1998	9972265
serotyping of flavobacterium meningosepticum by co-agglutination method.	the purpose of this investigation was to evaluate the usefulness of a co-agglutination procedure for the typing of flavobacterium meningosepticum. the sensitivity and specificity of the co-agglutination test was compared to the slide agglutination test using reference strains of the bacterial species. antisera were characterized by both technics to determine their titer and working dilution. the specificity of the sera was assessed by performing tests which include strains of other species and s ...	1998	10772579
chryseobacterium meningosepticum (flavobacterium meningosepticum)--a report of five cases in a local hospital.	chrysobacterium meningosepticum (flavobacterium meningosepticum) is a known cause of meningitis in premature and newborn infants. infection due to this organism in adults is uncommon. we report 5 cases of chrysobacterium meningosepticum in adult patients. most of these patients were elderly and had underlying co-morbidities.	1999	10672403
mutational analysis of bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase propeptide processing.	glutamine phosphoribosylpyrophosphate amidotransferase from bacillus subtilis is a member of an n-terminal nucleophile hydrolase enzyme superfamily, several of which undergo autocatalytic propeptide processing to generate the mature active enzyme. a series of mutations was analyzed to determine whether amino acid residues required for catalysis are also used for propeptide processing. propeptide cleavage was strongly inhibited by replacement of the cysteine nucleophile and two residues of an oxy ...	1999	10049369
flavobacterium meningosepticum sepsis in an infant with a diarrheal prodrome.	a full term, previously normal 2 1/2-month-old black boy was transferred to our hospital from an outlying facility on hospital day 5 for failure to thrive. three weeks before transfer, the infant was hospitalized for a diarrheal illness with fever. the baby received 3 days of ceftriaxone empirically and was discharged home after the sepsis evaluation was negative. mild diarrhea and steady weight loss continued and the baby was readmitted. blood culture done on admission grew flavobacterium menin ...	1999	10071673
prolyl endopeptidase inhibitors from the underground part of rhodiola sacra s. h. fu.	prolyl endopeptidase (pep, ec 3.4.21.26) is an enzyme which plays a role in the metabolism of proline-containing neuropeptides, e.g., vasopressin, substance p and thyrotropin-releasing hormone (trh), which have been suggested to be involved in learning and memory processes. in our systematic screening for pep inhibitors from traditional chinese medicines, we found that meoh extract from the underground part of rhodiola sacra s. h. fu shows significant inhibitory activity against pep from flavoba ...	1999	10077434
biochemical characterization of the pseudomonas aeruginosa 101/1477 metallo-beta-lactamase imp-1 produced by escherichia coli.	the blaimp gene coding for the imp-1 metallo-beta-lactamase produced by a pseudomonas aeruginosa clinical isolate (isolate 101/1477) was overexpressed via a t7 expression system in escherichia coli bl21 (de3), and its product was purified to homogeneity with a final yield of 35 mg/liter of culture. the structural and functional properties of the enzyme purified from e. coli were identical to those of the enzyme produced by p. aeruginosa. the imp-1 metallo-beta-lactamase exhibits a broad-spectrum ...	1999	10103197
detection of unculturable bacteria in periodontal health and disease by pcr.	recently developed molecular methods have made it possible to characterize mixed microflora in their entirety, including the substantial numbers of bacteria which do not grow on artificial culture media. in a previous study, molecular analysis of the microflora associated with acute oral infections resulted in the identification of three phylotypes, pus3.42, pus9.170, and pus9.180, representing as-yet-uncultured organisms. the aim of this study was to design and validate specific pcr primers for ...	1999	10203507
flavobacterium meningosepticum bacteremia: an analysis of 16 cases.	flavobacterium meningosepticum is an uncommon pathogen causing nosocomial pneumonia and meningitis in newborns. it is usually resistant to antimicrobial agents used to treat gram-negative bacilli. while the pathogen often results in high mortality and serious sequelae in newborns, it is also found to cause to disease in adults. therefore, it is necessary to know the full spectrum of the infection in adults and to identify effective antimicrobial agents.	1999	10222599
characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody c9.1 identifies an allelic form of the 2b4 antigen.	a newly generated monoclonal antibody (mab c9.1) described in this study identifies a surface membrane molecule that is involved in the lytic programme of activated natural killer (nk) cells. this conclusion is based on the facts that, first, this antigen was expressed on the vast majority of surface immunoglobulin (sig)- cd3- cd4- cd8- spleen lymphocytes, albeit it was also present on minor subsets of sig+ b (approximately 7%) and cd3+ t (approximately 2%) lymphocytes; second, that all splenic ...	1999	10233732
controlled clinical comparison of biomérieux vital and bactec nr-660 blood culture systems for detection of bacteremia and fungemia in adults.	a total of 9,446 blood cultures were collected from adult patients at three university-affiliated hospitals. of these, 8,943 cultures were received with both aerobic bottles filled adequately; 885 yielded 1,016 microorganisms, including 622 isolates (61%) that were the cause of sepsis, 337 isolates (33%) that were contaminants, and 57 isolates (6%) that were indeterminate as the cause of sepsis. with the exception of staphylococcus aureus, which was recovered more often from vital aerobic bottle ...	1999	10325312
neurotractin, a novel neurite outgrowth-promoting ig-like protein that interacts with cepu-1 and lamp.	the formation of axon tracts in nervous system histogenesis is the result of selective axon fasciculation and specific growth cone guidance in embryonic development. one group of proteins implicated in neurite outgrowth, fasciculation, and guidance is the neural members of the ig superfamily (igsf). in an attempt to identify and characterize new proteins of this superfamily in the developing nervous system, we used a pcr-based strategy with degenerated primers that represent conserved sequences ...	1999	10330412
dengue virus type 1 nonstructural glycoprotein ns1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion.	nonstructural glycoprotein ns1, specified by dengue virus type 1 (den-1), is secreted from infected green monkey kidney (vero) cells in a major soluble form characterized by biochemical and biophysical means as a unique hexameric species. this noncovalently bound oligomer is formed by three dimeric subunits and has a molecular mass of 310 kda and a stokes radius of 64.4 a. during protein export, one of the two oligosaccharides of ns1 is processed into an endo-beta-n-acetylglucosaminidase f-resis ...	1999	10364366
identification and management of an outbreak of flavobacterium meningosepticum infection in a colony of south african clawed frogs (xenopus laevis).	during the summer of 1996, an outbreak of flavobacterium meningosepticum infection developed in a colony of south african clawed frogs (xenopus laevis). clinical signs were consistent with septicemia: ascites, anasarca, dyspnea, extreme lethargy, congestion of web vessels, petechial hemorrhages, and sudden death. mortality rate reached 35%, and all infections were fatal. the organism was resistant to most antibiotics but was susceptible to enrofloxacin, chloramphenicol, and trimethoprim-sulfadia ...	1999	10382028
cloning and characterization of blavim, a new integron-borne metallo-beta-lactamase gene from a pseudomonas aeruginosa clinical isolate.	production of a metallo-beta-lactamase activity was detected in a carbapenem-resistant pseudomonas aeruginosa clinical isolate (isolate vr-143/97) from an italian inpatient at the verona university hospital (northern italy). the metallo-beta-lactamase determinant was isolated from a genomic library of vr-143/97, constructed in an escherichia coli plasmid vector, by screening for clones with reduced susceptibility to imipenem. sequencing of the cloned gene revealed that it encoded a new class b b ...	1999	10390207
variable carbohydrate modifications to the catalytic chains of the rgpa and rgpb proteases of porphyromonas gingivalis w50.	proteases of porphyromonas gingivalis are considered to be important virulence determinants of this periodontal bacterium. several biochemical isoforms of arginine-specific proteases are derived from rgpa and rgpb. hrgpa is a heterodimer composed of the catalytic alpha chain noncovalently associated with a beta adhesin chain derived from the c terminus of the initial full-length translation product. the catalytic alpha chain is also present as a monomer (rgpa) either free in solution or associat ...	1999	10417143
cloning of a chryseobacterium (flavobacterium) meningosepticum chromosomal gene (blaa(cme)) encoding an extended-spectrum class a beta-lactamase related to the bacteroides cephalosporinases and the veb-1 and per beta-lactamases.	in addition to the blab metallo-beta-lactamase, chryseobacterium (flavobacterium) meningosepticum ccug 4310 (nctc 10585) constitutively produces a 31-kda active-site serine beta-lactamase, named cme-1, with an alkaline isoelectric ph. the blaa(cme) gene that encodes the latter enzyme was isolated from a genomic library constructed in the escherichia coli plasmid vector pacyc184 by screening for cefuroxime-resistant clones. sequence analysis revealed that the cme-1 enzyme is a new class a beta-la ...	1999	10471563
expression of the isoamylase gene of flavobacterium odoratum ku in escherichia coli and identification of essential residues of the enzyme by site-directed mutagenesis.	the isoamylase gene from flavobacterium odoratum ku was cloned into and expressed in escherichia coli jm109. the promoter of the gene was successful in e. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. the enzyme found in the culture medium showed almost the same m(r), heat-inactivating constant, and n-terminal sequence as those of the enzyme accumulated in the periplasmic space. this result indicated that the enzyme accumulat ...	1999	10473430
purification and crystallization of precursors and autoprocessed enzymes of flavobacterium glycosylasparaginase: an n-terminal nucleophile hydrolase.	glycosylasparaginase (ga) represents a novel group of proteins that are activated by self-catalyzed peptide-bond cleavage from a single-chain precursor to yield the two subunits required for hydrolase activity. the wild-type ga precursor autoproteolyzes spontaneously into alpha and beta subunits. strategies are reported here for purification to homogeneity of ga from flavobacterium meningosepticum in both single-chain precursor and mature (autoprocessed) forms. the recombinant proteins crystalli ...	1999	10531509
novel bacterial peroxidase without catalase activity from flavobacterium meningosepticum: purification and characterization.	a novel bacterial peroxidase co-produced intracellularly with h(2)o(2)-forming nucleoside oxidase, was purified from the cell-free extract of flavobacterium meningosepticum to homogeneity with 10.3% overall recovery through simple purification procedures including successive deae-sephacel, phenyl-sepharose cl-4b and sephacryl s-300 chromatography. the relative molecular mass of the native enzyme was 220¿ omitted¿000 da, and that of its subunit was 54¿ omitted¿000 da. in contrast to other major i ...	1999	10561543
chryseobacterium meningosepticum bacteremia secondary to central intravenous line-related infection.		1999	10586158
cloning and nucleotide sequencing of prolyl endopeptidase gene from aeromonas punctata subsp. punctata.	prolyl endopeptidase activity was found in aeromonas punctata subsp. punctata. the genomic dna was partially digested with ecori and the recovered 8-16 kb dna fragments were inserted into the ecori site of plasmid puc19, and were transformated into escherichia coli dh5alpha. the resulted clones were screened by using benzyloxycarbonyl-gly-pro-beta-naphthylamide, the specific substrate of prolyl endopeptidase and a positive clone was obtained. the 12 kb insertion fragment of recombinant plasmid w ...	1999	12114972
an unusual case of flavobacterium meningosepticum pneumonia in an immunocompromised patient.	an unusual case of flavobacterium meningosepticum pneumonia in an immunocompromised patient is reported. the necessity to recognize this opportunistic pathogen and its implication is discussed.	1999	11127384
[post-splenectomy sepsis caused by flavobacterium meningosepticum].		2000	11149178
frequency of low-level bacteremia in children from birth to fifteen years of age.	a single blood culture inoculated with a small volume of blood is still frequently being used for the diagnosis of bacteremia in children because of the continued belief by many that bacteria are usually found in high concentrations in the blood of pediatric patients with sepsis. to determine the importance of both blood volume cultured and the number of culture devices required for the reliable detection of pathogens in our pediatric population, blood from children from birth to 15 years of age ...	2000	10834973
purification and characterization of barley dipeptidyl peptidase iv.	barley (hordeum vulgare l.) storage proteins, which have a high content of proline (pro) and glutamine, are cleaved by cysteine endoproteases to yield peptides with a pro next to the n-terminal and/or c-terminal amino acid residues. a peptidase cleaving after xaa-pro- at the n terminus of peptides was purified from green barley malt. it was identified as a serine-type dipeptidyl peptidase (dpp), based on inhibitor studies, and the nature of the cleavage product. it is a monomeric glycoprotein wi ...	2000	10677435
genetic-biochemical analysis and distribution of the ambler class a beta-lactamase cme-2, responsible for extended-spectrum cephalosporin resistance in chryseobacterium (flavobacterium) meningosepticum.	in vitro synergy between extended-spectrum cephalosporins and either clavulanic acid or cefoxitin was found for chryseobacterium meningosepticum isolates during a double-disk assay on an agar plate. an extended-spectrum beta-lactamase (esbl) gene from a c. meningosepticum clinical isolate was cloned and expressed in escherichia coli dh10b. its protein conferred resistance to most beta-lactams including extended-spectrum cephalosporins but not to cephamycins or to imipenem. its activity was stron ...	2000	10602714
isolation and expression in escherichia coli of csla and cslb, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase ac and chondroitinase b, respectively, from flavobacterium heparinum.	in medium supplemented with chondroitin sulfate, flavobacterium heparinum synthesizes and exports two chondroitinases, chondroitinase ac (chondroitin ac lyase; ec 4.2.2.5) and chondroitinase b (chondroitin b lyase; no ec number), into its periplasmic space. chondroitinase ac preferentially depolymerizes chondroitin sulfates a and c, whereas chondroitinase b degrades only dermatan sulfate (chondroitin sulfate b). the genes coding for both enzymes were isolated from f. heparinum and designated csl ...	2000	10618199
enzymatic properties of dipeptidyl aminopeptidase iv produced by the periodontal pathogen porphyromonas gingivalis and its participation in virulence.	porphyromonas gingivalis is a major pathogen associated with adult periodontitis. we cloned and sequenced the gene (dpp) coding for dipeptidyl aminopeptidase iv (dppiv) from p. gingivalis w83, based on the amino acid sequences of peptide fragments derived from purified dppiv. an escherichia coli strain overproducing p. gingivalis dppiv was constructed. the enzymatic properties of recombinant dppiv purified from the overproducer were similar to those of dppiv isolated from p. gingivalis. the thre ...	2000	10639438
production of an endo-beta-n-acetylglucosaminidase activity mediates growth of enterococcus faecalis on a high-mannose-type glycoprotein.	enterococcus faecalis is associated with a high proportion of nosocomial infections; however, little is known of the ability of this organism to proliferate in vivo. the ability of rnase b, a model glycoprotein with a single n-glycosylation site occupied by a family of high-mannose-type glycans (man(5)- to man(9)-glcnac(2)), to support growth of e. faecalis was investigated. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of rnase b demonstrated a reduction in the molecular mass of thi ...	2000	10648510
evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli.	the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ...	2000	10699007
characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france.	pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ...	2000	10722487
tla-1: a new plasmid-mediated extended-spectrum beta-lactamase from escherichia coli.	escherichia coli r170, isolated from the urine of an infected patient, was resistant to expanded-spectrum cephalosporins, aztreonam, ciprofloxacin, and ofloxacin but was susceptible to amikacin, cefotetan, and imipenem. this particular strain contained three different plasmids that encoded two beta-lactamases with pis of 7.0 and 9.0. resistance to cefotaxime, ceftazidime, aztreonam, trimethoprim, and sulfamethoxazole was transferred by conjugation from e. coli r170 to e. coli j53-2. the transfer ...	2000	10722503
activation of lipoxin a(4) receptors by aspirin-triggered lipoxins and select peptides evokes ligand-specific responses in inflammation.	lipoxin (lx) a(4) and aspirin-triggered lx (atl) are endogenous lipids that regulate leukocyte trafficking via specific lxa(4) receptors (alxrs) and mediate antiinflammation and resolution. atl analogues dramatically inhibited human neutrophil (polymorphonuclear leukocyte [pmn]) responses evoked by a potent necrotactic peptide derived from mitochondria as well as a rogue synthetic chemotactic peptide. these bioactive lipid analogues and small peptides each selectively competed for specific (3)h- ...	2000	10748237
carbapenemases of chryseobacterium (flavobacterium) meningosepticum: distribution of blab and characterization of a novel metallo-beta-lactamase gene, blab3, in the type strain, nctc 10016.	genes encoding carbapenemases in 15 reference strains of chryseobacterium (flavobacterium) meningosepticum from the united kingdom national collection of type cultures and in one recent clinical isolate were investigated. all the strains hydrolyzed imipenem, but their levels of resistance to carbapenems varied, with imipenem and meropenem mics ranging from 2 to >32 microg/ml. the blab gene, which encodes a molecular-class b carbapenemase, was detected in only six reference strains and in clinica ...	2000	10817691
the legionella (fluoribacter) gormanii metallo-beta-lactamase: a new member of the highly divergent lineage of molecular-subclass b3 beta-lactamases.	a metallo-beta-lactamase determinant was cloned from a genomic library of legionella (fluoribacter) gormanii atcc 33297(t) constructed in the plasmid vector pacyc184 and transformed into escherichia coli dh5alpha, by screening for clones showing a reduced susceptibility to imipenem. the product of the cloned determinant, named fez-1, contains a 30-kda polypeptide and exhibits an isoelectric ph of 7.6. sequencing revealed that fez-1 is a molecular-class b beta-lactamase which shares the closest s ...	2000	10817705
png1, a yeast gene encoding a highly conserved peptide:n-glycanase.	it has been proposed that cytoplasmic peptide:n-glycanase (pngase) may be involved in the proteasome-dependent quality control machinery used to degrade newly synthesized glycoproteins that do not correctly fold in the er. however, a lack of information about the structure of the enzyme has limited our ability to obtain insight into its precise biological function. a pngase-defective mutant (png1-1) was identified by screening a collection of mutagenized strains for the absence of pngase activit ...	2000	10831608
molecular and biochemical heterogeneity of class b carbapenem-hydrolyzing beta-lactamases in chryseobacterium meningosepticum.	although the carbapenem-hydrolyzing beta-lactamase (chbetal) blab-1 is known to be in chryseobacterium meningosepticum nctc 10585, a second chbetal gene, bla(gob-1), was cloned from another c. meningosepticum clinical isolate (pint). the g+c content of bla(gob-1) (36%) indicated the likely chromosomal origin of this gene. its expression in escherichia coli dh10b yields a mature chbetal with a pi of 8.7 and a relative molecular mass of 28.2 kda. in e. coli, gob-1 conferred resistance to narrow-sp ...	2000	10858348
rapid diagnosis of legionnaires' disease using an immunochromatographic assay for legionella pneumophila serogroup 1 antigen in urine during an outbreak in the netherlands.	a new immunochromatographic assay for rapid qualitative detection of legionella pneumophila serogroup 1 antigen in urine specimens was used during an outbreak of legionellosis in the netherlands. the assay seems of the utmost value in providing a rapid diagnosis of legionnaires' disease in patients with severe community-acquired pneumonia in an outbreak setting.	2000	10878074
characteristics of neonatal bacterial meningitis in a teaching hospital in taiwan from 1984-1997.	during the period from 1984 to 1997, 85 bacterial meningitis neonates with positive cerebrospinal fluid cultures were treated. the ages of these patients ranged from 1 to 28 days. the male to female ratio was 1.7 to 1. the most common causative agent was group b beta-hemolytic streptococci (gbs, 31.8%), followed by escherichia coli (20%), proteus mirabilis (7.1%), enterobacter cloacae (5.9%), other streptococci excluding streptococcus pneumoniae (5.9%), chryseobacterium meningosepticum (5.9%), e ...	2000	10917879
a novel protein-deamidating enzyme from chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil.	a novel protein-deamidating enzyme, which has potential for industrial applications, was purified from the culture supernatant of chryseobacterium proteolyticum strain 9670(t) isolated from rice field soil in tsukuba, japan. the deamidating activities on carboxybenzoxy (cbz)-gln-gly and caseins and protease activity were produced synchronously by the isolate. both deamidating activities were eluted as identical peaks separated from several proteases by phenyl-sepharose chromatography of the cult ...	2000	10919788
ibc-1, a novel integron-associated class a beta-lactamase with extended-spectrum properties produced by an enterobacter cloacae clinical strain.	a transferable beta-lactamase produced by a multidrug-resistant clinical isolate of enterobacter cloacae was studied. the bla gene was carried by a large (>80-kb) transmissible plasmid. nucleotide sequence analysis of cloned fragments revealed that it was part of a gene cassette carried by a class 1 integron along with other resistance genes, including aac(6')-ib. the encoded beta-lactamase, designated ibc-1, was a novel class a enzyme that hydrolyzed ceftazidime and cefotaxime and was inhibited ...	2000	10952563