Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| polymicrobial infection of the cornea due to contact lens wear. | a 38-year-old male presented with pain and redness in his left eye. he had a history of wearing contact lenses. his ophthalmic examination revealed a large corneal ulcer with surrounding infiltrate. cultures were isolated from the contact lenses, lens solutions, storage cases, and conjunctivae of both eyes and also corneal scrapings of the left eye. fortified vancomycin and amikacin drops were started hourly. culture results of conjunctivae of each eye and left cornea were positive for pseudomon ... | 2016 | 27800266 |
| association between elevated alanine aminotransferase and urosepsis in children with acute pyelonephritis. | the aim of this study is to investigate the association between elevated alanine aminotransferase (alt) and urosepsis in children with acute pyelonephritis (apn). | 2016 | 27066449 |
| mosquito c-type lectins maintain gut microbiome homeostasis. | the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ... | 2016 | 27170846 |
| draft genome sequences of strains representing each of the elizabethkingia genomospecies previously determined by dna-dna hybridization. | draft genome sequences of elizabethkingia meningoseptica and representatives of each of its four historically described genomospecies were sequenced here. preliminary analysis suggests that elizabethkingia miricola belongs to genomospecies 2, and both elizabethkingia anophelis and elizabethkingia endophytica are most similar to genomospecies 1. | 2016 | 26966213 |
| native conformation and canonical disulfide bond formation are interlinked properties of hiv-1 env glycoproteins. | we investigated whether there is any association between a native-like conformation and the presence of only the canonical (i.e., native) disulfide bonds in the gp120 subunits of a soluble recombinant human immunodeficiency virus type 1 (hiv-1) envelope (env) glycoprotein. we used a mass spectrometry (ms)-based method to map the disulfide bonds present in nonnative uncleaved gp140 proteins and native-like sosip.664 trimers based on the bg505 env gene. our results show that uncleaved gp140 protei ... | 2016 | 26719247 |
| biochemical characterization of cps-1, a subclass b3 metallo-β-lactamase from a chryseobacterium piscium soil isolate. | cps-1 is a subclass b3 metallo-β-lactamase from a chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the gob-1 enzyme. cps-1 was overproduced in escherichia coli rosetta (de3), purified by chromatography, and biochemically characterized. this enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of l1, gob-1, and acquired subclass b3 enzymes aim-1 and smb-1. | 2016 | 26666948 |
| comparative study of plasma endotoxin with procalcitonin levels in diagnosis of bacteremia in intensive care unit patients. | both procalcitonin (pct) and plasma endotoxin levels cannot be solely used for a definite diagnosis of bacteremia or sepsis, and there has been few study comparing the values of the two biomarkers for the diagnosis of bacteremia. the aim of this study was to identify bacteria causing bacteremia and evaluate the role of the two biomarkers in the diagnosis of bacteremia in intensive care unit (icu). | 2016 | 26879015 |
| n-glycosylation improves the pepsin resistance of histidine acid phosphatase phytases by enhancing their stability at acidic phs and reducing pepsin's accessibility to its cleavage sites. | n-glycosylation can modulate enzyme structure and function. in this study, we identified two pepsin-resistant histidine acid phosphatase (hap) phytases from yersinia kristensenii (ykappa) and yersinia rohdei (yrappa), each having an n-glycosylation motif, and one pepsin-sensitive hap phytase from yersinia enterocolitica (yeappa) that lacked an n-glycosylation site. site-directed mutagenesis was employed to construct mutants by altering the n-glycosylation status of each enzyme, and the mutant an ... | 2016 | 26637601 |
| expression, purification and biological characterization of the extracellular domain of cd40 from pichia pastoris. | cd40, also called bp50, is a novel member of the tnf receptor superfamily. based on its important role in multiple physiological and pathological processes, the cd40 signaling pathway has become a vital target for treating transplantation, autoimmune diseases and cancers. this study generated a protein fragment that disrupts this signaling pathway. | 2016 | 26809818 |
| taxonogenomics and description of vaginella massiliensis gen. nov., sp. nov., strain marseille p2517(t), a new bacterial genus isolated from the human vagina. | an obligate aerobic, gram-negative, nonmotile and nonsporulating rod designated marseille p2517 was isolated from the vaginal flora. we describe its features, annotate the genome and compare it to the closest species. the 16s rrna analysis shows 93.03% sequence similarity with weeksella virosa, the phylogenetically closest species. its genome is 2 434 475 bp long and presents 38.16% g+c. on the basis of these data, it can be considered as a new genus in the flavobacteriaceae family, for which we ... | 2016 | 28053707 |
| cp40 from corynebacterium pseudotuberculosis is an endo-β-n-acetylglucosaminidase. | c. pseudotuberculosis is an important animal pathogen that causes substantial economical loss in sheep and goat farming. zoonotic infections in humans are rare, but when they occur they are often severe and difficult to treat. one of the most studied proteins from this bacterium, the secreted protein cp40 is being developed as a promising vaccine candidate and has been characterized as a serine protease. in this study we have investigated if cp40 is an endoglycosidase rather than a protease. | 2016 | 27821068 |
| genome sequence of elizabethkingia meningoseptica em1, isolated from a patient with a bloodstream infection. | elizabethkingia meningoseptica em1 was isolated from a whole-blood sample from a female patient. the draft genome sequence of em1 contains 4,038,467 bp, with a g+c content of 36.37%. a preliminary genome analysis showed that em1 contains genes conferring resistance to β-lactams. the bacterium has hemolysin genes and a set of genes involved in heme uptake and heme utilization, showing its potential to cause bloodstream infections. a clustered regularly interspaced short palindromic repeat (crispr ... | 2016 | 27789634 |
| elevated plasma matrix metalloproteinase-9 and its correlations with severity of disease in patients with ventilator-associated pneumonia. | ventilator-associated pneumonia (vap) increases patient mortality and medical expenditure, and a real-time and reliable method for the rapid diagnosis of vap may help reduce fatal complications. matrix metalloproteinases-9 (mmp-9) is considered significant in the pathogenesis of lung inflammation and infection. therefore, we examined its relationship with the clinical course of vap. this retrospective observational study recruited 30 healthy volunteers, 12 patients who used mechanical ventilatio ... | 2016 | 27499696 |
| a complete profile on blind-your-eye mangrove excoecaria agallocha l. (euphorbiaceae): ethnobotany, phytochemistry, and pharmacological aspects. | traditional system of medicine consists of large number of plants with various medicinal and pharmacological importances. this article provides a comprehensive review of the complete profile of an important mangrove plant excoecaria agallocha l. (euphorbiaceae) and elaborately describing the ethnobotany, phytochemistry, and pharmacological properties. it is used traditionally in the treatment of various diseases such as epilepsy, ulcers, leprosy, rheumatism, and paralysis. the latex obtained fro ... | 2016 | 28082796 |
| complete genome sequences of four strains from the 2015-2016 elizabethkingia anophelis outbreak. | the complete circularized genome sequences of selected specimens from the largest known elizabethkingia anophelis outbreak to date are described here. genomic rearrangements observed among the outbreak strains are discussed. | 2016 | 27313304 |
| new β-lactamase inhibitors in the clinic. | given the serious medical burden of β-lactamases, many approaches are being used identify candidate agents for β-lactamase inhibition. here, we review two β-lactam-β-lactamase inhibitor (bl-bli) combinations, ceftolozane-tazobactam and ceftazidime-avibactam that recently entered the clinic. in addition, we focus on bl-bli combinations in preclinical development that have demonstrated activity in clinical isolates via susceptibility testing and/or in in vivo models of infection. we highlight only ... | 2016 | 27208767 |
| taxonomer: an interactive metagenomics analysis portal for universal pathogen detection and host mrna expression profiling. | high-throughput sequencing enables unbiased profiling of microbial communities, universal pathogen detection, and host response to infectious diseases. however, computation times and algorithmic inaccuracies have hindered adoption. | 2016 | 27224977 |
| ispd produces cdp-ribitol used by fktn and fkrp to transfer ribitol phosphate onto α-dystroglycan. | mutations in genes required for the glycosylation of α-dystroglycan lead to muscle and brain diseases known as dystroglycanopathies. however, the precise structure and biogenesis of the assembled glycan are not completely understood. here we report that three enzymes mutated in dystroglycanopathies can collaborate to attach ribitol phosphate onto α-dystroglycan. specifically, we demonstrate that isoprenoid synthase domain-containing protein (ispd) synthesizes cdp-ribitol, present in muscle, and ... | 2016 | 27194101 |
| the role of toll-like receptor 4 in infectious and noninfectious inflammation. | toll-like receptor 4 (tlr4) belongs to the family of pattern recognition receptors (prrs). they are highly conserved receptors that recognize conserved pathogen-associated molecular patterns (pamps), thus representing the first line of defense against infections. tlr4 has been long recognized as the sensing receptor for gram-negative lipopolysaccharide (lps). in addition, it also binds endogenous molecules produced as a result of tissue injury. hence, tlr4 represents a key receptor on which both ... | 2016 | 27293318 |
| elizabethkingia anophelis bacteremia is associated with clinically significant infections and high mortality. | unlike elizabethkingia meningoseptica, the clinical importance of e. anophelis is poorly understood. we determined the clinical and molecular epidemiology of bacteremia caused by elizabethkingia-like species from five regional hospitals in hong kong. among 45 episodes of elizabethkingia-like bacteremia, 21 were caused by elizabethkingia, including 17 e. anophelis, three e. meningoseptica and one e. miricola; while 24 were caused by other diverse genera/species, as determined by 16s rrna gene seq ... | 2016 | 27185741 |
| a gene of the β3-glycosyltransferase family encodes n-acetylglucosaminyltransferase ii function in trypanosoma brucei. | the bloodstream form of the human pathogen trypanosoma brucei expresses oligomannose, paucimannose, and complex n-linked glycans, including some exceptionally large poly-n-acetyllactosamine-containing structures. despite the presence of complex n-glycans in this organism, no homologues of the canonical n-acetylglucosaminyltransferase i or ii genes can be found in the t. brucei genome. these genes encode the activities that initiate the elaboration of the manα1-3 and manα1-6 arms, respectively, o ... | 2016 | 27189951 |
| evaluation of the filmarray blood culture identification panel: results of a multicenter controlled trial. | sepsis is a major cause of morbidity, mortality, and increased medical expense. rapid diagnosis improves outcomes and reduces costs. the filmarray blood culture identification panel (biofire diagnostics llc, salt lake city, ut), a highly multiplexed pcr assay, can identify 24 etiologic agents of sepsis (8 gram-positive, 11 gram-negative, and 5 yeast species) and three antimicrobial resistance genes (meca, vana/b, and blakpc) from positive blood culture bottles. it provides results in about 1 h w ... | 2016 | 26739158 |
| n-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (cdx1)/villin mrna expression. | various cancers such as colorectal cancer (crc) are associated with alterations in protein glycosylation. crc cell lines are frequently used to study these (glyco)biological changes and their mechanisms. however, differences between crc cell lines with regard to their glycosylation have hitherto been largely neglected. here, we comprehensively characterized the n-glycan profiles of 25 different crc cell lines, derived from primary tumors and metastatic sites, in order to investigate their potent ... | 2016 | 26537799 |
| assessment of biofilm formation of e. meningoseptica, d. acidovorans, and s. maltophilia in lens cases and their growth on recovery media. | bacterial biofilm formation in contact lens cases is a risk factor in the development of both microbial and infiltrative keratitis. this investigation evaluated three emerging pathogens: stenotrophomonas maltophilia, elizabethkingia meningoseptica, and delftia acidovorans for biofilm formation and metabolic activity in lens cases. also, growth of these bacteria on different media was assessed to optimize recovery conditions. | 2016 | 26383012 |
| outbreak of elizabethkingia meningoseptica sepsis with meningitis in a well-baby nursery. | between march and may 2012, three neonates born at a regional maternity hospital developed elizabethkingia meningoseptica (previously chryseobacterium meningosepticum) sepsis with meningitis aged <10 days, and were treated successfully with intravenous ciprofloxacin plus vancomycin or piperacillin-tazobactam for three to six weeks. four (16.6%) of 24 environmental specimens obtained from the nursery and delivery room were positive for this organism. all of the clinical isolates and two isolates ... | 2016 | 28077242 |
| endo-f3 glycosynthase mutants enable chemoenzymatic synthesis of core-fucosylated triantennary complex type glycopeptides and glycoproteins. | chemoenzymatic synthesis is emerging as a promising approach to the synthesis of homogeneous glycopeptides and glycoproteins highly demanded for functional glycomics studies, but its generality relies on the availability of a range of enzymes with high catalytic efficiency and well defined substrate specificity. we describe in this paper the discovery of glycosynthase mutants derived from elizabethkingia meningoseptica endoglycosidase f3 (endo-f3) of the gh18 family, which are devoid of the inhe ... | 2016 | 26966183 |
| infections due to elizabethkingia meningoseptica in critically injured trauma patients: a seven-year study. | elizabethkingia meningoseptica is an infrequent cause of hospital-acquired infections. the clinical and microbiological profiles of infections due to e. meningoseptica over a seven-year period at a level-i trauma centre are reported in this study. medical records of patients from whose clinical samples e. meningoseptica was isolated on more than one occasion were reviewed. a total of 21 cases were observed during the study, 16 (76.2%) of which exhibited multidrug resistance. the observed in-hosp ... | 2016 | 26763750 |
| microbial contamination of ice machines is mediated by activated charcoal filtration systems in a city hospital. | although microbial contamination of ice machines has been reported, no previous study has addressed microbial contamination of ice produced by machines equipped with activated charcoal (ac) filters in hospitals. the aim of this study was to provide clinical data for evaluating ac filters to prevent microbial contamination of ice. we compared microbial contamination in ice samples produced by machines with (n = 20) and without an ac filter (n = 40) in shunan city shinnanyo municipal hospital. all ... | 2016 | 27348980 |
| diversity of culturable gram-negative bacteria isolated from irrigation water of two rice crop regions in southern brazil. | in this study, we assessed the diversity of gram-negative bacteria found in water used for irrigation of rice crops in two growing areas of southern brazil. samples were collected from the main irrigation channel and field drain area. twenty-two bacterial species were found in cachoeirinha and 28 in camaquã. in both areas, the most frequent bacterial families were enterobacteriaceae and aeromonadaceae. differences in microbial diversity were observed in both study areas. thirty-five gram-negativ ... | 2016 | 27197729 |
| functional and structural analysis of a β-glucosidase involved in β-1,2-glucan metabolism in listeria innocua. | despite the presence of β-1,2-glucan in nature, few β-1,2-glucan degrading enzymes have been reported to date. recently, the lin1839 protein from listeria innocua was identified as a 1,2-β-oligoglucan phosphorylase. since the adjacent lin1840 gene in the gene cluster encodes a putative glycoside hydrolase family 3 β-glucosidase, we hypothesized that lin1840 is also involved in β-1,2-glucan dissimilation. here we report the functional and structural analysis of lin1840. a recombinant lin1840 prot ... | 2016 | 26886583 |
| pathogens in ornamental waters: a pilot study. | in parks, ornamental waters of easy access and populated with animals are quite attractive to children and yet might hide threats to human health. the present work focuses on the microbiota of the ornamental waters of a lisboa park, characterized during 2015. the results show a dynamic microbiota integrating human pathogens such as klebsiella pneumoniae, aeromonas spp. and enterobacter spp., and also antibiotic resistant bacteria. k. pneumoniae and aeromonas spp. were present as planktonic and b ... | 2016 | 26891309 |
| correction for pradeep et al., draft genome sequence of elizabethkingia anopheles, isolated from a postoperative endophthalmitis patient. | 2016 | 27932658 | |
| etymologia: elizabethkingia. | 2016 | 27057563 | |
| waterborne elizabethkingia meningoseptica in adult critical care. | elizabethkingia meningoseptica is an infrequent colonizer of the respiratory tract; its pathogenicity is uncertain. in the context of a 22-month outbreak of e. meningoseptica acquisition affecting 30 patients in a london, uk, critical care unit (3% attack rate) we derived a measure of attributable morbidity and determined whether e. meningoseptica is an emerging nosocomial pathogen. we found monomicrobial e. meningoseptica acquisition (n = 13) to have an attributable morbidity rate of 54% (syste ... | 2016 | 26690562 |
| taxonogenomics and description of vaginella massiliensis gen. nov., sp. nov., strain marseille p2517(t), a new bacterial genus isolated from the human vagina. | an obligate aerobic, gram-negative, nonmotile and nonsporulating rod designated marseille p2517 was isolated from the vaginal flora. we describe its features, annotate the genome and compare it to the closest species. the 16s rrna analysis shows 93.03% sequence similarity with weeksella virosa, the phylogenetically closest species. its genome is 2 434 475 bp long and presents 38.16% g+c. on the basis of these data, it can be considered as a new genus in the flavobacteriaceae family, for which we ... | 2016 | 28053707 |
| mechanistic and evolutionary insights from comparative enzymology of phosphomonoesterases and phosphodiesterases across the alkaline phosphatase superfamily. | naively one might have expected an early division between phosphate monoesterases and diesterases of the alkaline phosphatase (ap) superfamily. on the contrary, prior results and our structural and biochemical analyses of phosphate monoesterase pafa, from chryseobacterium meningosepticum, indicate similarities to a superfamily phosphate diesterase [xanthomonas citri nucleotide pyrophosphatase/phosphodiesterase (npp)] and distinct differences from the three metal ion ap superfamily monoesterase, ... | 2016 | 27670607 |
| successful management of elizabethkingia meningoseptica meningitis with intraventricular vancomycin. | elizabethkingia meningoseptica, formerly chryseobacterium meningosepticum usually causes neonatal meningitis and is a rare cause of nosocomial meningitis in adults. e. meningoseptica is resistant to most antibiotics, and the use of inactive drugs as empirical therapy may contribute to poor outcome in many patients. vancomycin, alone or in combination with rifampicin, has been successful in the treatment of meningitis in infants1. we present a case of e. meningoseptica meningitis in an adult who ... | 2016 | 27766817 |
| first report of bacteremia caused by elizabethkingia meningoseptica in a dog. | 2016 | 27587896 | |
| crystal structure of the metallo-β-lactamase gob in the periplasmic dizinc form reveals an unusual metal site. | metallo-beta-lactamases (mbls) are broad-spectrum, zn(ii)-dependent lactamases able to confer resistance to virtually every β-lactam antibiotic currently available. the large diversity of active-site structures and metal content among mbls from different sources has limited the design of a pan-mbl inhibitor. gob-18 is a divergent mbl from subclass b3 that is expressed by the opportunistic gram-negative pathogen elizabethkingia meningoseptica this mbl is atypical, since several residues conserved ... | 2016 | 27458232 |
| elizabethkingia meningoseptica and contact lens use. | a 47-year-old man presented to our emergency department complaining of acute ocular pain and redness of his left eye. ophthalmological antecedents included use of semi-rigid contact lens and primary open-angle glaucoma. slit-lamp examination revealed a small central corneal ulcer associated with stromal inflammatory infiltrate. scraping from the corneal ulcer was positive for elizabethkingia meningoseptica. he was empirically treated with topical 0.5% levofloxacin and 0.3% gentamicin, and five w ... | 2016 | 27349783 |
| risk factors and outcome of levofloxacin-resistant elizabethkingia meningoseptica bacteraemia in adult patients in taiwan. | elizabethkingia meningoseptica is an emerging nosocomial pathogen associated with high mortality and inherently resistant to many antimicrobial agents. levofloxacin has been considered as a therapeutic agent based on in vitro susceptibility. we aim to investigate the risk factors and outcomes for levofloxacin-resistant e. meningoseptica bacteraemia. adult patients with e. meningoseptica bacteraemia were identified retrospectively in a medical centre in taiwan from january 2011 to july 2015. thes ... | 2017 | 28265817 |
| relative prevalence and antimicrobial susceptibility of clinical isolates of elizabethkingia species based on 16s rrna gene sequencing. | some of the previously reported clinical isolates of elizabethkingia meningoseptica may be later named species of elizabethkingia we determined the accuracy of species identification (with two matrix-assisted laser desorption ionization-time of flight mass spectrometry [maldi-tof ms] systems and the vitek 2 gn card), relative prevalence of three elizabethkingia spp. in clinical specimens, and antimicrobial susceptibility of the species identified by 16s rrna gene sequencing. specimens for cultur ... | 2017 | 27847376 |
| under-utilization of taps in intensive care unit as a cause of reservoirs of nonfermenting gram-negative bacilli. | the under-utilisation of taps is associated with the generation of reservoirs of non-fermenting gram-negative bacilli with the ability to disseminate. we describe the detection and approach of the problem in an icu. | 2017 | 28291668 |
| isolation of methicillin-resistant staphylococcus aureus and multidrug-resistant elizabethkingia meningoseptica from neonates within minutes of birth. | 2017 | 27941472 | |
| genome of the carbapenemase-producing clinical isolate elizabethkingia miricola em_chuv and comparative genomics with elizabethkingia meningoseptica and elizabethkingia anophelis: evidence for intrinsic multidrug resistance trait of emerging pathogens. | elizabethkingia miricola is a gram-negative non-fermenting rod emerging as a life-threatening human pathogen. the multidrug-resistant (mdr) carbapenemase-producing clinical isolate e. miricola em_chuv was recovered in the setting of severe nosocomial pneumonia. in this study, the genome of e. miricola em_chuv was sequenced and a functional analysis was performed, including a comparative genomic study with elizabethkingia meningoseptica and elizabethkingia anophelis. the resistome of em_chuv reve ... | 2017 | 27913093 |
| emergence of carbapenem resistant non-fermenting gram-negative bacilli isolated in an icu of a tertiary care hospital. | the emergence and spread of multi-drug resistant (mdr) non-fermenting gram-negative bacilli (nfgnb) in intensive care units (icu) and their genetic potential to transmit diverse antibiotic resistance regardless of their ability to ferment glucose poses a major threat in hospitals. the complex interplay of clonal spread, persistence, transmission of resistance elements and cell-cell interaction leads to the difficulty in controlling infections caused by these multi drug-resistant strains. among n ... | 2017 | 28273965 |