| tributyltin-resistant marine bacteria: a summary of recent work. | a tributyltin chloride (tbtcl)-resistant bacterium, alteromonas sp. m-1, was isolated from coastal seawater. this bacterium grew in medium containing 125 microm tbtcl. tbtcl added to the medium was taken up by this bacterium, however, the amount of tbtcl in the cellular fraction was low after the logarithmic phase, suggesting the existence of a tbtcl-efflux system. a genetic library was constructed using plasmid vector puc 19. three positive clones were obtained, by which e. coli was transformed ... | 1995 | 7766207 |
| flow cytometric analysis of the cellular dna content of salmonella typhimurium and alteromonas haloplanktis during starvation and recovery in seawater. | flow cytometry was used to investigate the heterogeneity of the dna content of salmonella typhimurium and alteromonas haloplanktis cells that were starved and allowed to recover in seawater. hoechst 33342 (bisbenzimide) was used as a dna-specific dye to discriminate between dna subpopulations. the dna contents of both strains were heterogeneous during starvation. s. typhimurium cells contained one or two genomes, and a. haloplanktis cells contained up to six genomes. s. typhimurium genomes were ... | 1994 | 7811075 |
| [distribution of alpha-n-acetylgalactosaminidases in marine and freshwater microorganisms]. | the distribution of the alpha-n-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the pacific ocean and 423 baikalian microorganisms from fresh water and sponges have been studied. about 24% as marine as baikalian strains produced this enzyme. the most number of alpha-n-ac-galactosidase producers were found for marine bacteria genera aeromonas, alteromonas and vibrio (71% a ... | 1994 | 7898396 |
| sequence similarities and evolutionary relationships of microbial, plant and animal alpha-amylases. | amino acid sequence comparison of 37 alpha-amylases from microbial, plant and animal sources was performed to identify their mutual sequence similarities in addition to the five already described conserved regions. these sequence regions were examined from structure/function and evolutionary perspectives. an unrooted evolutionary tree of alpha-amylases was constructed on a subset of 55 residues from the alignment of sequence similarities along with conserved regions. the most important new infor ... | 1994 | 7925367 |
| characterization of an antibiotic produced by alteromonas luteoviolacea gauthier 1982, 85 isolated from kinko bay, japan. | an antibiotic produced by alteromonas luteoviolacea strain 9k-v10 was recovered after cold acetone precipitation of culture supernatant fluids or lysates that had been frozen and thawed. the precipitate obtained from cell-free lysates was fractioned by deae ion-exchange chromatography. further purification by gel-filtration chromatography yielded a single peak of antibiotic activity that corresponded to a protein peak with a molecular mass of approximately 100 kda. after non-denaturing polyacryl ... | 1994 | 7989271 |
| sodium ion-substrate symport in a marine bacterium. | anaerobic suspensions of alteromonas haloplanktis accumulated alpha-aminoisobutyric acid, by a sodium-dependent process, in response to an artificially imposed membrane potential in the presence or absence of a transmembrane chemical gradient of sodium. these results suggest that the transport of alpha-aminoisobutyrate by this organism occurs via na+-substrate symport. | 1980 | 7380801 |
| isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities. | lytic digestion of poly(mannuronate), poly(guluronate), and alginate with an alginate lyase from alteromonas macleodii was used to prepare mixtures of unsaturated oligosaccharides. four oligosaccharides isolated from the alginate lyase-lysate by anion-exchange chromatography on q-sepharose were found to be the major components of the root growth-promoting lysate. the oligosaccharides were analyzed by nmr and sims and identified as di- and tri-saccharides having o-(4-deoxy-l-erythro-hex-4-enopyra ... | 1994 | 8039175 |
| gene sequence, purification and characterization of n-acetyl-beta-glucosaminidase from a marine bacterium, alteromonas sp. strain o-7. | the gene (cht60) encoding n-acetyl-beta-glucosaminidase (cht; ec 3.2.1.30) from the marine bacterium alteromonas sp. strain o-7 was cloned into puc18 in escherichia coli jm109. the nucleotide (nt) sequence of cht60 was determined. a 1797-bp open reading frame encoded a polypeptide of 598 amino acids (aa) (m(r) 64,535). the aa sequence of the cloned enzyme (cht60) deduced from the nt sequence showed no significant sequence homologies with available aa sequences from databases. cht60 was purified ... | 1994 | 8063094 |
| the gene encoding the kappa-carrageenase of alteromonas carrageenovora is related to beta-1,3-1,4-glucanases. | the nucleotide (nt) and deduced amino acid (aa) sequences are reported for the structural gene cgka encoding kappa-carrageenase (m(r) 44,412) from the marine bacterium alteromonas carrageenovora (atcc 43555), a hydrolase involved in the degradation of kappa-carrageenan. the cgka gene codes for a protein of 397 aa, with a signal peptide of 25 aa. the enzyme is a new member of family 16 of glycosyl hydrolases, which comprises beta-1,3-1,4-glucanases from various sources and the beta-agarase of str ... | 1994 | 8112578 |
| dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31p nmr. | the dynamic properties of nucleic acids in five different types of intact supramolecular systems, namely, chicken erythrocyte chromatin, the wild type and a deletion mutant of the lambda phage, lipid-containing phage pm2, and alteromonas espejiana ribosomes, were investigated by means of 31p solid-state nmr. the nucleic acids in the different supramolecular systems showed unique dynamic properties, which are closely connected with their functions. the total anisotropy of the phosphorus chemical ... | 1994 | 8206876 |
| lipid composition and chemotaxonomy of pseudomonas putrefaciens (alteromonas putrefaciens). | the major polar lipids in cells of pseudomonas putrefaciens ncib 10472 grown on nutrient agar were phosphatidylethanolamine, phoisphatidylglycerol, a glucosyldiacylglycerol, a glucuronosyldiacylglycerol and an ornithine amide lipid. an additional phospholipid, tentatively identified as acyl phosphatidylglycerol or bis-phosphatidic acid, was a trace component of the wall lipids from broth cultures, which lacked the glycolipids and the ornithine amide lipid. the wall lipids from broth cultures of ... | 1980 | 7441198 |
| effect of phosphate concentration on production of tetrodotoxin by alteromonas tetraodonis. | tetrodotoxin production by alteromonas tetraodonis occurred during the stationary phase of growth and was regulated by phosphate concentration; toxin production was repressed if phosphate was added at the onset of stationary phase and was over 100-fold greater in phosphate-limited cultures than in cultures in which phosphate was not limiting. | 1993 | 8285702 |
| a pluripotent polyphenol oxidase from the melanogenic marine alteromonas sp shares catalytic capabilities of tyrosinases and laccases. | the recently characterized marine melanogenic bacterium mmb-1 contains a pluripotent polyphenol oxidase (ppo) which catalyzes the oxidation of a very wide range of substrates considered specific for tyrosinase or laccase. this range includes monophenols such as l-tyrosine, o-diphenols such as l-dopa, p-diphenols such as hydroquinone, o-aminophenols such as 3-hydroxyanthranilic acid, activated monophenols such as 2,6-dimethoxyphenol and syringaldazine, and chromophores such as abts. this is the f ... | 1997 | 9398646 |
| cloning and sequence of an alkaline serine protease-encoding gene from the marine bacterium alteromonas sp. strain o-7. | the gene (aprii) encoding alkaline serine protease (aprii; subtilase) from alteromonas sp. strain o-7 was cloned in plasmid puc19 and transformed into escherichia coli jm109. the nucleotide (nt) sequence of aprii has been determined. a single open reading frame (orf) encoded a protein consisting of 621 amino acids (aa) with a m(r) of 63,958. the results of aa sequence analysis indicated that aprii is produced as a large precursor consisting of four domains: the signal sequence, the n-terminal pr ... | 1993 | 8294010 |
| thiomarinol, a new hybrid antimicrobial antibiotic produced by a marine bacterium. fermentation, isolation, structure, and antimicrobial activity. | thiomarinol, an antimicrobial antibiotic, was isolated from the culture broth of a marine bacterium, alteromonas rava sp. nov. sank 73390. its structure was deduced as a hybrid composed of a pseudomonic acid analogue and holothin by nmr spectral analysis and chemical degradation. antimicrobial activity against gram-positive and gram-negative bacteria of thiomarinol was stronger than both of pseudomonic acids and pyrrothine antibiotics. | 1993 | 8294241 |
| cloning of gene responsible for tributyltin chloride (tbtc1) resistance in tbtc1-resistant marine bacterium, alteromonas sp. m-1. | a genetic library of tributyltin chloride (tbtc1)-resistant marine bacterium, alteromonas sp. m-1, was constructed using plasmid vector puc 19. three positive clones were obtained from e. coli jm 109 transformed with the plasmids by the method of replica plating to lb medium containing 1 mm tbtc1. these clones could grow in lb liquid medium containing 100 microm tbtc1. plasmids harbouring genes of alteromonas sp. m-1 were designated ptbt1, ptbt2 and ptbt3 which contain 1.8 kb hind iii-fragment, ... | 1993 | 8343157 |
| screening of halophilic bacteria and alteromonas species for organophosphorus hydrolyzing enzyme activity. | previously, a g-type nerve agent degrading enzyme activity was found in a halophilic bacterial isolate designated jd6.5. this organism was tentatively identified as an unknown species of the genus alteromonas. in order to determine whether this type of enzyme activity was common in other species of alteromonas, a screening program was initiated. a number of alteromonas species and five halophilic bacterial isolates were cultured and their crude cell extracts screened for hydrolytic activity agai ... | 1993 | 8393735 |
| cloning, sequence, and expression of a chitinase gene from a marine bacterium, altermonas sp. strain o-7. | the gene encoding an extracellular chitinase from marine alteromonas sp. strain o-7 was cloned in escherichia coli jm109 by using puc18. the chitinase produced was not secreted into the growth medium but accumulated in the periplasmic space. a chitinase-positive clone of e. coli produced two chitinases with different molecular weights from a single chitinase gene. these proteins showed almost the same enzymatic properties as the native chitinase of alteromonas sp. strain o-7. the n-terminal sequ ... | 1993 | 8416892 |
| phylogenetic relationships of marine bacteria, mainly members of the family vibrionaceae, determined on the basis of 16s rrna sequences. | the phylogenetic relationships of 50 reference strains, mostly marine bacteria which require na+ for growth, were determined on the basis of 600 16s rrna nucleotides by using reverse transcriptase sequencing. strains belonging to 10 genera were included (four genera of the family vibrionaceae, the genus aeromonas of the family aeromonadaceae, and the genera alteromonas, marinomonas, shewanella, pseudomonas, and deleya). the sequences were aligned, the similarity values and evolutionary distance ... | 1993 | 8427811 |
| purification and characterization of the alpha-agarase from alteromonas agarlyticus (cataldi) comb. nov., strain gj1b. | the phenotypic features of strain gj1b, an unidentified marine bacterium that degrades agar [young, k. s. bhattacharjee, s. s. & yaphe, w. (1978) carbohydr. res. 66, 207-212], were investigated and its agarolytic system was characterized using 13c-nmr spectroscopy to analyse the agarose degradation products. the bacterium was assigned to the genus alteromonas and the new combination a. agarlyticus (cataldi) is proposed. an alpha-agarase, i.e. specific for the alpha(1-->3) linkages present in aga ... | 1993 | 8513809 |
| sialic acid-binding lectin with antibacterial activity from the horse mussel: further characterization and immunolocalization. | a heterogeneous sialic acid-binding lectin with affinity for bacterial lps was isolated and partially characterized from hemolymph of the horse mussel modiolus modiolus.(1) using two-dimensional electrophoresis with immobilized ph gradients, the lectin revealed three subunits with different molecular weight and isoelectric points (pi); mr14 (pi approximately 5.1 and approximately 5.5), 17.5 (pi approximately 5.5) and 20 (pi approximately 4.9) kda. the affinity purified lectin existed in its nati ... | 1998 | 9639084 |
| a comparative study of pcr product detection and quantitation by electro-chemiluminescence and fluorescence. | amplification and detection of target dna sequences are made possible in a polymerase chain reaction (pcr) by using a mixture of biotinylated and ruthenium(ii) trisbipyridal (ru(bpy)3(2+))-end-labelled primers. in this way, biotin for capture and ru(bpy)3(2+) for detection are directly incorporated into the pcr product obviating subsequent probe hybridization. pcr of a bacterial dna template from alteromonas species strain jd6.5 using a cocktail of biotin- and ru(bpy)3(2+)-labelled primers ampli ... | 1995 | 8533605 |
| arylsulphatase from alteromonas carrageenovora. | arylsulphatase activity was identified in cultures of the marine bacterium alteromonas carrageenovora, using methylumbelliferyl sulphate as substrate. in contrast with most other microbial arylsulphatases, arylsulphatase production in a. carrageenovora was not repressed by sulphate. the structural gene of arylsulphatase (atsa) was cloned and sequenced. an orf of 984 bp was found, specifying a primary translation product of 328 amino acids with a molecular mass of 35797 da. arylsulphatase was par ... | 1995 | 8535517 |
| description of a new polymer-secreting bacterium from a deep-sea hydrothermal vent, alteromonas macleodii subsp. fijiensis, and preliminary characterization of the polymer. | a deep-sea, aerobic, mesophilic, heterotrophic bacterium was isolated from fluid collected near an active hydrothermal vent. on the basis of phenotypic and phylogenetic analyses and dna-dna relatedness, strain st716 could be assigned to the species alteromonas macleodii as a new subspecies. this bacterium secreted an unusual high-molecular-weight polysaccharide in the presence of glucose in batch cultures. the viscosity of this exopolysaccharide is of the same order of magnitude as that of xanth ... | 1996 | 8572714 |
| cloning and expression of a gene encoding a bacterial enzyme for decontamination of organophosphorus nerve agents and nucleotide sequence of the enzyme. | organophosphorus acid (opa) anhydrolase enzymes have been found in a wide variety of prokaryotic and eukaryotic organisms. interest in these enzymes has been prompted by their ability to catalyze the hydrolysis of toxic organophosphorus cholinesterase-inhibiting compounds, including pesticides and chemical nerve agents. the natural substrates for these enzymes are unknown. the gene (opaa) which encodes an opa anhydrolase (opaa-2) was isolated from an alteromonas sp. strain jd6.5 ecori-lambda zap ... | 1996 | 8633861 |
| structural and functional aspects of chloride binding to alteromonas haloplanctis alpha-amylase. | chloride is the allosteric effector of vertebrate pancreatic and salivary alpha-amylases and of the bacterial alpha-amylase from alteromonas haloplanctis. activation experiments of a. haloplanctis alpha-amylase by several monovalent anions show that a negative charge, not restricted to that of cl-, is essential for the amylolytic reaction. engineering of the chloride binding site reveals that a basic residue is an essential component of the site. the mutation k337r alters the cl--binding propert ... | 1996 | 8798613 |
| structure of the capsular polysaccharide from alteromonas sp. cmm 155. | capsular polysaccharide (cps) was obtained by water-saline extraction of the alteromonas sp. cmm 155. on the basis of solvolysis with anhydrous hf and 1h- and 13c-nmr spectral data, including noe experiments, it was concluded that the capsular polysaccharide had the following structure containing novel n-acyl-amino sugar and bacillosamine residues: --> 3)-alpha-d-galpnac-(1 --> 4)-alpha-l-galapnac(1 --> 3)- alpha-d-quipnac4nac-(1 --> 3)-beta-d-quip4nalaac-(1 --> | 1995 | 7585719 |
| characterization and solution properties of a new exopolysaccharide excreted by the bacterium alteromonas sp. strain 1644. | this paper concerns the study of a new bacterial exopolysaccharide. the ionic properties of the polymer are considered in relation to the first structural results. this polysaccharide is a carboxylic polyelectrolyte with a charge parameter of 1.33, and exhibits usual polyelectrolyte properties with monovalent and mg2+ ions. in contrast, its affinity for the divalent ions ca2+, sr2+ and ba2+ is greater than predicted from electrostatic theory. in solution, the polysaccharide undergoes a local con ... | 1996 | 8852748 |
| structure of the capsular polysaccharide from alteromonas nigrifaciens iam 13010t containing 2-acetamido-2,6-dideoxy-l-talose and 3-deoxy-d-manno-octulosonic acid. | a capsular polysaccharide was obtained from alteromonas nigrifaciens iam 13010t by saline extraction. on the basis of 1h and 13c nmr spectroscopy, including one-dimensional (1d) noe spectroscopy, 2d rotating-frame noe spectroscopy (roesy), and 1h-detected heteronuclear 1h,13c multiple-quantum coherence (hmqc), it was concluded that the polysaccharide contained inter alia an acidic sugar, 3-deoxy-d-manno-octulosonic acid (kdo), and a rare amino sugar, 2-acetamido-2,6-dideoxy-l-talose (l-6dtalnac, ... | 1997 | 9129296 |
| alteromonas infernus sp. nov., a new polysaccharide-producing bacterium isolated from a deep-sea hydrothermal vent. | a deep-sea, aerobic, mesophilic and heterotrophic new bacterium was isolated from a sample of fluid collected among a dense population of riftia pachyptila, in the vicinity of an active hydrothermal vent of the southern depression of the guaymas basin (gulf of california). on the basis of phenotypic and phylogenetic analyses and dna/dna relatedness, the strain gy785 was recognized as a new species of the genus alteromonas and the name of alteromonas infernus is proposed. during the stationary ph ... | 1997 | 9134716 |
| a novel metalloproteinase, almelysin, from a marine bacterium, alteromonas sp. no. 3696: purification and characterization. | we have discovered a novel metalloproteinase, which has high activity at low temperatures, from the culture supernatant of a marine bacterium. the strain was identified as alteromonas sp. no. 3696. the metalloproteinase, named almelysin, was purified to homogeneity from the cultured supernatant at 10 degrees c by two column chromatographies. about 20 mg of purified almelysin was obtained from 18.4 liters of the culture supernatant. the molecular mass of almelysin was estimated to be 28 kda by sd ... | 1997 | 9145531 |
| diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
| shewanella woodyi sp. nov., an exclusively respiratory luminous bacterium isolated from the alboran sea. | thirty-four strains of nonfermentative, respiratory, luminous bacteria were isolated from samples of squid ink and seawater from depths of 200 to 300 m in the alboran sea. although these strains had a few properties similar to properties of shewanella (alteromonas) hanedai, they did not cluster phenotypically with any previously described bacterium. the nucleotide sequence of a 740-bp segment of luxa was not homologous with other known luxa sequences but clustered with the luxa sequences of shew ... | 1997 | 9336902 |
| stability and structural analysis of alpha-amylase from the antarctic psychrophile alteromonas haloplanctis a23. | the alpha-amylase secreted by the antarctic bacterium alteromonas haloplanctis displays 66% amino acid sequence similarity with porcine pancreatic alpha-amylase. the psychrophilic alpha-amylase is however characterized by a sevenfold higher kcat and kcat/km values at 4 degrees c and a lower conformational stability estimated as 10 kj.mol-1 with respect to the porcine enzyme. it is proposed that both properties arise from an increase in molecular flexibility required to compensate for the reducti ... | 1994 | 8020481 |
| specific inhibition of chemiluminescent activity by pathogenic vibrios in hemocytes of two marine bivalves: pecten maximus and crassostrea gigas. | hemocytes from two adult bivalves, pecten maximus and crassostrea gigas, were exposed to 12 different bacterial strains including 2 alteromonas spp. (u1 and t413), 2 type strains of vibrios (v. anguillarum atcc 19264 and v. alginolyticus atcc 17749), 1 vibrio (s322) pathogenic to c. gigas larvae, 2 vibrios (v110 and v117) virulent to ostrea edulis larvae, and 5 different strains of a same vibrio sp. (group a496) isolated from moribund p. maximus larvae. after 1.5 h contact with bacteria, zymosan ... | 1998 | 9446738 |
| characterization of chitinase c from a marine bacterium, alteromonas sp. strain o-7, and its corresponding gene and domain structure. | one of the chitinase genes of alteromonas sp. strain o-7, the chitinase c-encoding gene (chic), was cloned, and the nucleotide sequence was determined. an open reading frame coded for a protein of 430 amino acids with a predicted molecular mass of 46,680 da. alignment of the deduced amino acid sequence demonstrated that chic contained three functional domains, the n-terminal domain, a fibronectin type iii-like domain, and a catalytic domain. the n-terminal domain (59 amino acids) was similar to ... | 1998 | 9464381 |
| crystal structures of the psychrophilic alpha-amylase from alteromonas haloplanctis in its native form and complexed with an inhibitor. | alteromonas haloplanctis is a bacterium that flourishes in antarctic sea-water and it is considered as an extreme psychrophile. we have determined the crystal structures of the alpha-amylase (aha) secreted by this bacterium, in its native state to 2.0 angstroms resolution as well as in complex with tris to 1.85 angstroms resolution. the structure of aha, which is the first experimentally determined three-dimensional structure of a psychrophilic enzyme, resembles those of other known alpha-amylas ... | 1998 | 9541387 |
| phenotypic diversity of pseudoalteromonas citrea from different marine habitats and emendation of the description. | four strains of marine, aerobic, agar-decomposing bacteria with one polar flagellum and with dna g + c contents of 38.9-40.2 mol% were isolated from the far-eastern mussels crenomytilus grayanus and patinopecten yessoensis. these four strains were identified as pseudoalteromonas; however, they were phenotypically different from species described previously according to carbon compound utilization tests and the biolog identification system. high agar-decomposing activity was found in two strains, ... | 1998 | 9542094 |
| characterization of the c-terminal propeptide involved in bacterial wall spanning of alpha-amylase from the psychrophile alteromonas haloplanctis. | the antarctic psychrophile alteromonas haloplanctis secretes a ca2+- and cl--dependent alpha-amylase. the nucleotide sequence of the amy gene and the amino acid sequences of the gene products indicate that the alpha-amylase precursor is a preproenzyme composed by the signal peptide (24 residues), the mature alpha-amylase (453 residues, 49 kda), and a long c-terminal propeptide or secretion helper (192 residues, 21 kda). in cultures of the wild-type strain, the 70-kda precursor is secreted at the ... | 1998 | 9575155 |
| development of a genetic transformation system for an alga-lysing bacterium. | four marine bacteria, alteromonas sp. strains a27, a28, a29, and a30, that lyse the diatom skeletonema costatum nies-324 were isolated from coastal seawater samples. they were also able to lyse the diatoms thalassiosira sp. and eucampia zodiacs and the raphidophycean flagellate chattonella antiqua. cryptic indigenous plasmids, designated pas28 and pas29, were detected in alteromonas sp. strains a28 and a29, respectively. these plasmids appeared to be similar based on size and restriction site an ... | 1998 | 9603814 |
| structure determination of a novel uronic acid residue isolated from the exopolysaccharide produced by a bacterium originating from deep sea hydrothermal vents. | the exopolysaccharide produced by the bacterium alteromonas sp. strain 1644 originating from deep sea hydrothermal vents was shown to contain a novel glucuronic acid derivatives: 3-o-[(r)-1-carboxyethyl]-d-glucuronic acid. the structure of this compound was established on the basis of mass spectrometric data, methylation analysis, preparation of derivatives, and chemical synthesis of references compounds. | 1996 | 8823906 |
| pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of laminaria japonica. | an aerobic, polarly flagellated marine bacterium that produces a prodigiosin-like pigment was isolated from the red-spotted culture beds of laminaria japonica. five isolates had unique bacteriolytic activity for both gram-positive and -negative bacteria, which had never been observed among alteromonas or related species. the isolates were identified as the causative agent of red spot disease of l. japonica seeds. the phenotypic features of the isolates were similar to these of pseudoalteromonas ... | 1998 | 9734030 |
| structural studies of an exopolysaccharide produced by alteromonas macleodii subsp. fijiensis originating from a deep-sea hydrothermal vent. | the structure of the exopolysaccharide produced by alteromonas macleodii subsp. fijiensis recovered from a deep-sea hydrothermal vent has been investigated. by means of chemical analysis and nmr studies, the repeating unit of the polymer was deduced to be a branched hexasaccharide with the structure shown. [formula: see text] | 1998 | 9836450 |
| structures of the psychrophilic alteromonas haloplanctis alpha-amylase give insights into cold adaptation at a molecular level. | . enzymes from psychrophilic (cold-adapted) microorganisms operate at temperatures close to 0 degreesc, where the activity of their mesophilic and thermophilic counterparts is drastically reduced. it has generally been assumed that thermophily is associated with rigid proteins, whereas psychrophilic enzymes have a tendency to be more flexible. | 1998 | 9862804 |
| diversity of free-living and attached bacteria in offshore western mediterranean waters as depicted by analysis of genes encoding 16s rrna. | in a previous study (s. g. acinas, f. rodríguez-valera, and c. pedrós-alió, fems microbiol. ecol. 24:27-40, 1997), community fingerprinting by 16s rdna restriction analysis applied to mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 micrometer. here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16s rdna obtai ... | 1999 | 9925576 |
| g-type nerve agent decontamination by alteromonas prolidase. | | 1998 | 9928098 |
| cloning and nucleotide sequence of the gene encoding a serine proteinase inhibitor named marinostatin from a marine bacterium, alteromonas sp. strain b-10-31. | the gene (msti) encoding a serine proteinase inhibitor named marinostatin from marine alteromonas sp. strain b-10-31 was cloned and its nucleotide sequence was analyzed. a short open reading frame of 192 bp encoded 63 amino acids with a molecular weight of 6,985. furthermore, the initial product of marinostatin (marinostatin l) was purified and its amino acid sequence was analyzed. these results indicate that marinostatin is produced as a unique precursor consisting of the mature peptide and the ... | 1998 | 9972273 |
| solution and gel rheology of a new polysaccharide excreted by the bacterium alteromonas sp. strain 1644. | a new bacterial polysaccharide '1644' is studied in aqueous solutions and in the gel state. the viscometric measurements for dilute and semi-dilute solutions in presence of monovalent salts are usual; however, in presence of divalent cations like ca2+ an original behaviour is observed, related to intermolecular bonds. for larger polymer concentrations a clear gel is obtained with divalent counterions. this gel exhibits unusual properties compared to other gelling polysaccharides: it is very elas ... | 1996 | 8852757 |
| crystallization and preliminary x-ray diffraction studies of alpha-amylase from the antarctic psychrophile alteromonas haloplanctis a23. | a cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile alteromonas haloplanctis a23 grown at 4 degrees c. in order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold-adapted enzyme have been initiated because a three-dimensional structure of a mesophilic counterpart, pig pancreatic alpha-amylase, already exists. alpha-amylase from a. haloplanctis, which shares 53% sequence identity with pi ... | 1996 | 8897615 |
| molecular cloning and characterization of a novel beta-n-acetyl-d-glucosaminidase from vibrio furnissii. | the accompanying papers (keyhani, n. o., and roseman, s. (1996) j. biol. chem. 271, 33414-33424; keyhani, n. o., and roseman, s. (1996) j. biol. chem. 271, 33425-33432) describe two unique beta-n-acetylglucosaminidases from vibrio furnissii. a third, exoii, is reported here. the gene, exoii, was cloned into escherichia coli, sequenced, and exoii purified to apparent homogeneity (36 kda). the molecular weight and n-terminal 16 amino acids of the protein conform to the predicted sequence. exoii ex ... | 1996 | 8969206 |
| cloning and sequence analysis of a protease-encoding gene from the marine bacterium alteromonas sp. strain o-7. | the gene (apri) encoding alkaline serine protease (apri; subtilase) from alteromonas sp. strain o-7 was cloned and sequenced. the nucleotide sequence of apri has been identified. the deduced amino acid sequence indicated that apri codes for a precursor of 715 amino acids and the precursor is composed of four regions including a signal peptide, an n-terminal pro-region, a mature protease region and a c-terminal extension region of 215 amino acids as previously described for aprii [h. tsujibo et a ... | 1996 | 8987544 |
| nucleotide sequence of a gene encoding an organophosphorus nerve agent degrading enzyme from alteromonas haloplanktis. | organophosphorus acid anhydrolases (opaa) catalyzing the hydrolysis of a variety of toxic organophosphorus cholinesterase inhibitors offer potential for decontamination of g-type nerve agents and pesticides. the gene (opa) encoding an opaa was cloned from the chromosomal dna of alteromonas haloplanktis atcc 23821. the nucleotide sequence of the 1.7 -kb dna fragment contained the opa gene (1.3 kb) and its flanking region. we report structural and functional similarity of opaas from a. haloplankti ... | 1997 | 9079288 |
| alteromonas prolidase for organophosphorus g-agent decontamination. | enzymes catalyzing the hydrolysis of highly toxic organophosphorus compounds (ops) are classified as organophosphorus acid anhydrolases (opaa; ec 3.1.8.2). recently, the genes encoding opaa from two species of alteromonas were cloned and sequenced. sequence and biochemical analyses of the cloned genes and enzymes have established alteromonas opaas to be prolidases (e.c. 3.4.13.9), a type of dipeptidase hydrolyzing dipeptides with a prolyl residue in the carboxyl-terminal position (x-pro). altero ... | 1999 | 10421483 |
| purification and characterization of the heat-labile alpha-amylase secreted by the psychrophilic bacterium tac 240b. | a total of 59 bacteria samples from antarctic sea water were collected and screened for their ability to produce alpha-amylase. the highest activity was recorded from an isolate identified as an alteromonas species. the purified alpha-amylase shows a molecular mass of about 50,000 da and a pi of 5.2. the enzyme is stable from ph 7.5 to 9 and has a maximal activity at ph 7.5. compared with other alpha-amylases from mesophiles and thermophiles, the "cold enzyme" displays a higher activity at low t ... | 1999 | 10453473 |
| molecular analysis of the gene encoding a novel transglycosylative enzyme from alteromonas sp. strain o-7 and its physiological role in the chitinolytic system. | we purified from the culture supernatant of alteromonas sp. strain o-7 and characterized a transglycosylating enzyme which synthesized beta-(1-->6)-(glcnac)2, 2-acetamido-6-o-(2-acetamido-2-deoxy-beta-d-glucopyranosyl)-2- deoxyglucopyranose from beta-(1-->4)-(glcnac)2. the gene encoding a novel transglycosylating enzyme was cloned into escherichia coli, and its nucleotide sequence was determined. the molecular mass of the deduced amino acid sequence of the mature protein was determined to be 99, ... | 1999 | 10464221 |
| the effect of motility and cell-surface polymers on bacterial attachment. | recently it was shown that motility of vibrio alginolyticus facilitated cell attachment to glass surfaces. in the present study the same relationship between motility and cell attachment was confirmed for alcaligenes and alteromonas spp. these findings clearly answer a long-standing question: does motility facilitate attachment? however, they are contradictory to a general view on cell attachment that the energy barrier due to electrostatic repulsion between negatively charged bacterial cells an ... | 1999 | 10537201 |
| bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rrna gene restriction analysis and sequence analysis of 16s rrna genes. | the nearly universal colonization of surfaces in marine waters by bacteria and the formation of biofilms and biofouling communities have important implications for ecological function and industrial processes. however, the dynamics of surface attachment and colonization in situ, particularly during the early stages of biofilm establishment, are not well understood. experimental surfaces that differed in their degrees of hydrophilicity or hydrophobicity were incubated in a salt marsh estuary tida ... | 2000 | 10653705 |
| novel alginate lyases from marine bacterium alteromonas sp. strain h-4. | a bacterium alteromonas sp. strain h-4 isolated from laminaria fronds produced extra- and intra-cellular alginate lyases and utilized alginate as its sole carbon source. an extracellular alginate lyase was purified from the culture supernatant of the strain and its substrate specificity was characterized. the estimated molecular mass of the enzyme was 32 kda and the isoelectric point was 4.7. both polym and polyg block degrading activities were observed using the substrate-containing gel overlay ... | 1997 | 9403996 |
| idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the pacific ocean, including descriptions of two species, idiomarina abyssalis sp. nov. and idiomarina zobellii sp. nov. | two bacterial strains, kmm 227t and 231t, were isolated from seawater samples collected from the north-western pacific ocean at a depth of 4000-5000 m and were characterized using polyphasic taxonomy. both were gram-negative, psychrotolerant, heterotrophic, aerobic and required nacl for growth (0.6-15.0%). the temperature for growth was 4-30 degrees c. both strains were rod-shaped, with a single flagellum. however, strain kmm 231t revealed a single long fimbrium. cellular fatty acids detected in ... | 2000 | 10758902 |
| the alpha-amylase gene amyh of the moderate halophile halomonas meridiana: cloning and molecular characterization. | two types of tn1732-induced mutants defective in extracellular amylase activity were isolated from the moderate halophile halomonas meridiana dsm 5425. type i mutants displayed amylase activity in the periplasm, and were unable to use any of the carbon sources tested, including starch and its hydrolysis product maltose. the type ii mutant was affected in the gene responsible for the synthesis of the extracellular alpha-amylase. this gene (amyh) was isolated by functional complementation of mutan ... | 2000 | 10784044 |
| cloning and sequencing of the gene encoding an aldehyde dehydrogenase that is induced by growing alteromonas sp. strain ke10 in a low concentration of organic nutrients. | the protein composition of alteromonas sp. strain ke10 cultured at two different organic-nutrient concentrations was determined by using two-dimensional polyacrylamide gel electrophoresis. the cellular levels of three proteins, olga, -b, and -c, were considerably higher in cells grown in a low concentration of organic nutrient medium (lon medium; 0.2 mg of carbon per liter) than cells grown in a high concentration of organic nutrient medium (hon; 200 mg of c liter(-1)) or cells starved for organ ... | 2000 | 10788355 |
| successional changes in the genetic diversity of a marine bacterial assemblage during confinement. | the successional changes in the genetic diversity of mediterranean bacterioplankton subjected to confinement were studied in an experimental 300 1 seawater enclosure. five samples were taken at different times and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) fingerprinting to rapidly monitor changes in the bacterial genetic diversity. dgge analysis clearly showed variations between the samples. three of the five samples, with different dgge banding pat ... | 2000 | 10795685 |
| purification and characterization of n-acetylglucosamine-6-phosphate deacetylase from a psychrotrophic marine bacterium, alteromonas species. | a psychrotrophic bacterium, strain mct-9, which produced an n-acetylglucosamine-6-phosphate deacetylase, was isolated from a deep-seawater sample in the mariana trough. the mct-9 strain was identified as alteromonas sp. the native enzyme had a molecular mass of 164,000 da, and was predicted to be composed of four identical subunits with molecular masses of 41,000 da. the purified enzyme hydrolyzed n-acetylglucosamine (glcnac), glcnac-6-phosphate, and glcnac-6-sulfate. considering the low k(m) an ... | 2000 | 10804240 |
| expression, purification, crystallization and preliminary x-ray analysis of the iota-carrageenase from alteromonas fortis. | this is the first crystallization report of a glycoside hydrolase which belongs to family 82. a recombinant form of his-tagged iota-carrageenase from alteromonas fortis was expressed, purified and crystallized. crystals were obtained by the vapour-diffusion method using polyethylene glycol (m(w) = 6000) as a precipitant. they belong to space group p2(1), with unit-cell parameters a = 56. 75, b = 91.04, c = 125.01 a, beta = 93.41 degrees. the unit cell contains two molecules in the asymmetric uni ... | 2000 | 10818359 |
| reclassification of alteromonas distincta romanenko et al. 1995 as pseudoalteromonas distincta comb. nov. | the 16s rrna gene of alteromonas distincta kmm 638t was amplified, cloned and sequenced. the nucleotide sequence was aligned with sequences of representative strains of alteromonas, moritella, pseudoalteromonas and shewanella. results of phylogenetic analysis, using neighbour-joining and fitch-margoliash methods, clearly indicated that this species should be assigned to the genus pseudoalteromonas. on the basis of polyphasic data obtained from previous work and this study, it is proposed that th ... | 2000 | 10826797 |
| assignment of alteromonas elyakovii kmm 162t and five strains isolated from spot-wounded fronds of laminaria japonica to pseudoalteromonas elyakovii comb. nov. and the extended description of the species. | a marine bacterium, alteromonas elyakovii kmm 162t, which was described recently, and five strains isolated from spot-wounded fronds of laminaria japonica have been subjected to phylogenetic analysis, and geno- and phenotypic characterization. the phenotypic features of pseudoalteromonas elyakovii strains were closely related to that of pseudoalteromonas espejiana iam 12640t, but utilization of three carbon compounds (d-mannose, l-tyrosine and trehalose) distinguished both species. the g+c conte ... | 2000 | 10826813 |
| a dna ligase from the psychrophile pseudoalteromonas haloplanktis gives insights into the adaptation of proteins to low temperatures. | the cloning, overexpression and characterization of a cold-adapted dna ligase from the antarctic sea water bacterium pseudoalteromonas haloplanktis are described. protein sequence analysis revealed that the cold-adapted ph dna ligase shows a significant level of sequence similarity to other nad+-dependent dna ligases and contains several previously described sequence motifs. also, a decreased level of arginine and proline residues in ph dna ligase could be involved in the cold-adaptation strateg ... | 2000 | 10848966 |
| substrate and stereochemical specificity of the organophosphorus acid anhydrolase from alteromonas sp. jd6.5 toward p-nitrophenyl phosphotriesters. | the enzyme opaa hydrolyzes p-nitrophenyl phosphotriesters bearing substituents at the phosphorus center ranging in size from methyl to phenyl. the enzyme exhibits stereoselectivity toward the hydrolysis of chiral substrates with a preference for the sp enantiomer. | 2000 | 10866401 |
| culturability and in situ abundance of pelagic bacteria from the north sea. | the culturability of abundant members of the domain bacteria in north sea bacterioplankton was investigated by a combination of various cultivation strategies and cultivation-independent 16s rrna-based techniques. we retrieved 16s rrna gene (rdna) clones from environmental dnas and determined the in situ abundance of different groups and genera by fluorescence in situ hybridization (fish). a culture collection of 145 strains was established by plating on oligotrophic medium. isolates were screen ... | 2000 | 10877804 |
| in vitro studies on sterically stabilized liposomes (sl) as enzyme carriers in organophosphorus (op) antagonism. | this study describes a new approach for organophosphorous (op) antidotal treatment by encapsulating an op hydrolyzing enzyme, opa anhydrolase (opaa), within sterically stabilized liposomes. the recombinant opaa enzyme was derived from alteromonas strain jd6. it has broad substrate specificity to a wide range of op compounds: dfp and the nerve agents, soman and sarin. liposomes encapsulating opaa (sl)* were made by mechanical dispersion method. hydrolysis of dfp by (sl)* was measured by following ... | 2000 | 10892408 |
| differentiation of chitinase-active and non-chitinase-active subpopulations of a marine bacterium during chitin degradation. | the ability of marine bacteria to adhere to detrital particulate organic matter and rapidly switch on metabolic genes in an effort to reproduce is an important response for bacterial survival in the pelagic marine environment. the goal of this investigation was to evaluate the relationship between chitinolytic gene expression and extracellular chitinase activity in individual cells of the marine bacterium pseudoalteromonas sp. strain s91 attached to solid chitin. a green fluorescent protein repo ... | 2000 | 10919822 |
| production and separation of alpha-agarase from altermonas agarlyticus strain gj1b. | this paper presents results on the production of alpha-agarase by a fermentation process and its separation using membrane microfiltration (mf). optimization of fermentation conditions for alpha-agarase production using altermonas agarlyticus grown on medium containing agar as a carbon source was done in batch, fed-batch and continuous fermentations. continuous culture at a dilution rate of 0.03 h(-1) appeared to be best suited for production of alpha-agarase by this organism. at 0.03 h(-1) dilu ... | 2001 | 11396907 |
| thermodynamic stability of a cold-active alpha-amylase from the antarctic bacterium alteromonas haloplanctis. | the thermal stability of the cold-active alpha-amylase (aha) secreted by the antarctic bacterium alteromonas haloplanctis has been investigated by intrinsic fluorescence, circular dichroism, and differential scanning calorimetry. it was found that this heat-labile enzyme is the largest known multidomain protein exhibiting a reversible two-state unfolding, as demonstrated by the recovery of deltahcal values after consecutive calorimetric transitions, a deltahcal/deltaheff ratio close to unity, an ... | 1999 | 10194383 |
| spatial and temporal variations in chitinolytic gene expression and bacterial biomass production during chitin degradation. | growth of the chitin-degrading marine bacterium s91 on solid surfaces under oligotrophic conditions was accompanied by the displacement of a large fraction of the surface-derived bacterial production into the flowing bulk aqueous phase, irrespective of the value of the surface as a nutrient source. over a 200-h period of surface colonization, 97 and 75% of the bacterial biomass generated on biodegradable chitin and a nonnutritional silicon surface, respectively, detached to become part of the fr ... | 2000 | 10919823 |
| [screening of marine bacteria for fucoidan hydrolases]. | twenty-five strains of epiphytic marine bacteria isolated from the brown algae fucus evanescens and chorda filum and fifty-three bacteria isolated from the sea cucumber apostichopus japonicus were screened for fucoidanases using fucoidans prepared from the brown algae f. evanescens, laminaria cichorioides, and l. japonica. eighteen bacterial epiphytes and thirty-eight bacterial isolates from the sea cucumber were found to contain fucoidanases, which were able to hydrolyze either all of the fucoi ... | 2000 | 10920807 |
| iota-carrageenases constitute a novel family of glycoside hydrolases, unrelated to that of kappa-carrageenases. | iota-carrageenases are polysaccharide hydrolases that cleave the beta-1,4 linkages between the d-galactose-4-sulfate and 3, 6-anhydro-d-galactose-2-sulfate residues in the red algal galactans known as iota-carrageenans. we report here on the purification of iota-carrageenase activity from the marine bacterium zobellia galactanovorans and on the characterization of iota-carrageenase structural genes. genomic libraries from this latter bacterium as well as from alteromonas fortis were functionally ... | 2000 | 10934194 |
| numerical taxonomy of heavy metal-tolerant nonhalophilic bacteria isolated from hypersaline environments. | a total of 232 metal-tolerant bacterial strains were isolated from water and sediment samples collected in different hypersaline environments located in cádiz, huelva and morón de la frontera (spain). they were isolated on a medium containing mercury, chromium, cadmium, copper or zinc. these halotolerant isolates were analyzed by numerical taxonomy techniques by using the simple matching (ssm) and jaccard (sj) coefficients; clustering was achieved using the unweighted pair group method with aver ... | 1998 | 10943340 |
| long circulating liposomes encapsulating organophosphorus acid anhydrolase in diisopropylfluorophosphate antagonism. | these studies are focused on antagonizing organophosphorous (op) intoxications by a new conceptual approach using recombinant enzymes encapsulated within sterically stabilized liposomes to enhance diisopropylfluorophosphate (dfp) degradation. the op hydrolyzing enzyme, organophosphorous acid anhydrolase (opaa), encapsulated within the liposomes, was employed either alone or in combination with pralidoxime (2-pam) and/or atropine. the recombinant opaa enzyme, from the alteromonas: strain jd6, has ... | 2000 | 10966507 |
| metabolites from a marine bacterium pseudomonas/alteromonas, associated with the sponge dysidea fragilis. | the lipophylic extract from a black sea bacterium, associated with the sponge dysidea fragilis, was investigated. saturated hydrocarbons and fatty acids of the lipids were identified. the concentrations of the polycyclic compounds appeared to be negligible. the main components appeared to be phosphatidyl ethanolamine, followed by phosphatidyl serine. the first was investigated by fab mass spectrometry and a series of molecular species partially identified. | 2000 | 11007181 |
| evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of alteromonas-like proteobacteria. | the cellular phospholipids (pls) and fatty acids (fas) were investigated in type and environmental strains of pseudoalteromonas, alteromonas macleodii, a. infernus, and in three type strains of marinomonas, m. communis, m. vaga, m. mediterranea. a total of 40 strains (19 strains in this study and 21 reported in previous papers), including idiomarina abyssalis, i. zobellii, and glaciecola punicea, g. pallidula, aerobic alteromonas-like proteobacteria showed genus-characteristic patterns of phosph ... | 2000 | 11014871 |
| alteration of a single tryptophan residue of the cellulose-binding domain blocks secretion of the erwinia chrysanthemi cel5 cellulase (ex-egz) via the type ii system. | cel5 (formerly known as endoglucanase z) of erwinia chrysanthemi is secreted by the out type ii pathway. previous studies have shown that the catalytic domain (cd), linker region (lr) and cellulose-binding domain (cbd) each contain information needed for secretion. the aim of this work was to further investigate the secretion-related information present in the cbd(cel5). firstly(, )deleting a surface-exposed flexible loop had no effect on secretion. this indicated that some structural freedom is ... | 2000 | 11023779 |
| a siderophore from a marine bacterium with an exceptional ferric ion affinity constant. | virtually all microorganisms require iron for growth. the paucity of iron in surface ocean water (approximately 0.02-1.0 nm (refs 1, 2)) has spurred a lively debate concerning iron limitation of primary productivity, yet little is known about the molecular mechanisms used by marine microorganisms to sequester iron. terrestrial bacteria use a siderophore-mediated ferric uptake system. a siderophore is a low-molecular-mass compound with a high affinity for ferric ion which is secreted by microorga ... | 1993 | 8247152 |
| [spontaneous mutants of alteromonas espejiana, resistant to kanamycin and bleomycin]. | an attempt was made to induce insertions in marine bacterium alteromonas espejiana bal-31 (ae) using the tnphoa transposon. the ae mutants selected on a kanamycin-containing medium after conjugation of the ae with the transposon donor, escherichia coli sm10(prt291), were resistant not only to kanamycin (kn), but also to bleomycin (bm), and were sensitive to tetracycline. although the mutants were phenotypically similar to insertion mutants, the mutations appeared to be spontaneous. the sensitivi ... | 1999 | 10687100 |
| succession of pelagic marine bacteria during enrichment: a close look at cultivation-induced shifts. | enrichment experiments with north sea bacterioplankton were performed to test if rapid incubation-induced changes in community structure explain the frequent isolation of members of a few particular bacterial lineages or if readily culturable bacteria are common in the plankton but in a state of dormancy. a metabolic inhibitor of cell division (nalidixic acid [na]) was added to substrate-amended (s+) and unamended (s-) grazer-free seawater samples, and shifts in community composition and per cel ... | 2000 | 11055904 |
| a beta-amino acid containing tripeptide from a pseudomonas-alteromonas bacterium associated with a black sea sponge. | a novel tripeptide, 1, was isolated from the extracellular extract of a pseudomonas-alteromonas bacterium that was associated with the black sea sponge dysidea fragilis. compound 1 contains the novel beta-aminopimelic acid described for the first time from a natural product. the structure of 1 is suggested on the basis of the analysis of spectroscopic data and chemical degradation. | 2000 | 11076579 |
| fatty acid composition of bacteria associated with the toxic dinoflagellate ostreopsis lenticularis and with caribbean palythoa species. | the fatty acid composition of a pseudomonas sp. (alteromonas) and its host, the dinoflagellate ostreopsis lenticularis, vectors in ciguatera fish poisoning, has been studied. the major fatty acids in o. lenticularis were 16:0, 20:5n-3, and 22:6n-3, but 18:2n-6, 18:3n-3, and 18:n-3 were also identified. in contrast to other dinoflagellates, 1 8:5n-3 was not detected in o. lenticularis. even-chain fatty acids such as 9-16:1, 11-18:1, and 13-20:1 predominated in the pseudomonas sp. from o. lenticul ... | 1998 | 9655379 |
| protective effect caused by the exopolymer excreted by pseudoalteromonas antarctica nf(3) on liposomes against the action of octyl glucoside. | the capacity of the glycoprotein (gp) excreted by pseudoalteromonas antarctica nf(3), to protect phosphatidylcholine (pc) liposomes against the action of octyl glucoside (og) was studied in detail. increasing amounts of gp assembled with liposomes resulted for the same interaction step in a linear increase in the effective surfactant to pc molar ratios (re) and in a linear fall in the surfactant partitioning between bilayer and the aqueous phase (partition coefficients k). thus, the higher the p ... | 2000 | 11036228 |
| molecular cloning of the gene encoding an outer-membrane-associated beta-n-acetylglucosaminidase involved in chitin degradation system of alteromonas sp. strain o-7. | the gene encoding beta-n-acetylglucosaminidase (glcnacasea) was cloned using pcr with degenerate oligonucleotide primers from the partial amino acid sequence of the enzyme. the gene encoded a polypeptide of 863 amino acids with a predicted molecular mass of 97kda. a characteristic signal peptide, which was present at the amino-terminus of the precursor protein, contained four amino acids (ala-gly-cys-ser) identical in sequence and location to the processing and modification sites of the outer me ... | 2000 | 11193430 |
| the evolutionary history of chromosomal super-integrons provides an ancestry for multiresistant integrons. | integrons are genetic elements that acquire and exchange exogenous dna, known as gene cassettes, by a site-specific recombination mechanism. characterized gene cassettes consist of a target recombination sequence (attc site) usually associated with a single open reading frame coding for an antibiotic resistance determinant. the affiliation of multiresistant integrons (mris), which contain various combinations of antibiotic resistance gene cassettes, with transferable elements underlies the rapid ... | 2001 | 11209061 |
| structure of an acidic polysaccharide from a marine bacterium pseudoalteromonas distincta kmm 638 containing 5-acetamido-3,5,7,9-tetradeoxy-7-formamido-l-glycero-l-manno-nonulosonic acid. | an acidic polysaccharide was obtained from the lipopolysaccharide of pseudoalteromonas distincta strain kmm 638, isolated from a marine sponge, and found to contain d-glca, d-galnac, 2-acetamido-2,6-dideoxy-d-glucose (d-quinac) and two unusual acidic amino sugars: 2-acetamido-2-deoxy-d-galacturonic acid (d-galnaca) and 5-acetamido-3,5,7,9-tetradeoxy-7-formamido-l-glycero-l-manno-nonulosonic acid (pse5ac7fo, a derivative of pseudaminic acid). oligosaccharides were derived from the polysaccharide ... | 2001 | 11217976 |
| phylogenetic analysis of the succession of bacterial communities in the great south bay (long island). | bacterial community composition and succession were examined over the course of the summer season in the great south bay, long island, ny, usa, using a 16s rdna clone library approach. there was a progression of changes in dominant species in the libraries during the summer of 1997. the july library had several groups dominant, the sar407 relatives of the alpha-proteobacteria (24%) and the sar86 (18%), sulfur-oxidizing symbiont relatives (8%) of the gamma-proteobacteria, and unidentified cytopha ... | 2001 | 11248393 |
| purification and characterization of bifunctional alginate lyase from alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates. | a marine bacterium (strain no. 272) isolated from sea mud in omura bay produced an alginate lyase and was classified as an alteromonas species. the enzyme was purified from the culture medium of the bacterium by deae-cellulofine, sephadex g-100 gel chromatography to an electrophoretically homogeneous state in the presence and absence of sds. the molecular mass of the enzyme was 23 and 33.9 kda on sephadex g-100 column chromatography and sds-polyacrylamide gel electrophoresis, respectively, with ... | 2001 | 11272816 |
| use of a chia probe for detection of chitinase genes in bacteria from the chesapeake bay(1). | pcr primers specific for the chia gene were designed by alignment and selection of highly conserved regions of chia sequences from serratia marcescens, alteromonas sp., bacillus circulans and aeromonas caviae. these primers were used to amplify a 225 bp fragment of the chia gene from vibrio harveyi to produce a chia gene probe. the chia pcr primers and probe were used to detect the presence of the chia gene in an assemblage of 53 reference strains and gave consistent results. selected chia fragm ... | 2000 | 11053737 |
| [a new family of alteromonadaceae fam. nov., including the marine proteobacteria species alteromonas, pseudoalteromonas, idiomarina i colwellia]. | the taxonomic position of the marine genera alteromonas, pseudoalteromonas, idiomarina, and colwellia within the gamma subclass of the class proteobacteria were specified on the basis of their phenotypic, genotypic, and phylogenetic characteristics. gram-negative aerobic bacteria of the genera alteromonas, pseudoalteromonas, and idiomarina and facultatively anaerobic bacteria of the genus colwellia were found to form a phylogenetic cluster with a 16s rrna sequence homology of 90% or higher. the ... | 2001 | 11338830 |
| isolation and characterization of the genes encoding two metalloproteases (mpri and mprii) from a marine bacterium, alteromonas sp. strain o-7. | an extracellular alkaline metalloprotease (mpri) from alteromonas sp. strain o-7 was purified and characterized. the molecular mass of the purified enzyme was estimated to be 56 kda by sds-page. the optimum ph and temperature were ph 10.0 and 60 degrees c, respectively. the gene (mpri) encoding mpri was cloned and its nucleotide sequence was analyzed. the deduced amino acid sequence of mpri showed significant similarity to metalloproteases classified into the thermolysin family. furthermore, seq ... | 2002 | 11999419 |
| [tolerance of marine proteobacteria pseudoalteromonas and alteromonas to heavy metals compounds]. | | 2001 | 11386064 |
| characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16s rdna sequences. | one hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophylic conditions from arctic (98 strains) and antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. by numerical analysis, 8 clusters, containing 2 to 59 strains, could be delineated, and 8 strains formed separate branches. five clusters contained strains from both poles, two minor clusters were confined to arctic ... | 2001 | 11403404 |
| retrieval of the species alteromonas tetraodonis simidu et al. 1990 as pseudoalteromonas tetraodonis comb. nov. and emendation of description. | a polyphasic taxonomy study was undertaken of three strains of pseudoalteromonas haloplanktis subsp. tetraodonis (simidu et al. 1990) gauthier et al. 1995. dna was prepared from each of the strains and genomic relatedness was measured by dna-dna hybridization. strains kmm 458t and iam 14160t shared 99% genetic relatedness, but were only 48-49% related to the type strain of pseudoalteromonas haloplanktis subsp. haloplanktis, iam 12915t. the third strain, p. haloplanktis subsp. tetraodonis a-m, sh ... | 2001 | 11411675 |
| use of pcr-rflp for genotyping 16s rrna and characterizing bacteria cultured from halibut fry. | small subunit ribosomal genes were explored using pcr-rflp to facilitate the characterization of bacteria cultured from reared fry of the atlantic halibut (hippoglossus hippoglossus). concern has been expressed about pathogen invasion in larvae lacking a counteracting normal flora that may aid the immune system in producing robust noninfected individuals. in this study, pure cultured representatives of normal flora that were previously found to be antagonistic towards a pathogenic vibrio sp. wer ... | 2002 | 12109877 |
| the kappa-carrageenase of p. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of clan-b glycoside hydrolases. | kappa-carrageenans are gel-forming, sulfated 1,3-alpha-1,4-beta-galactans from the cell walls of marine red algae. the kappa-carrageenase from the marine, gram-negative bacterium pseudoalteromonas carrageenovora degrades kappa-carrageenan both in solution and in solid state by an endoprocessive mechanism. this beta-galactanase belongs to the clan-b of glycoside hydrolases. | 2001 | 11435116 |