Publications

purification and characterization of n-acetylglucosamine-6-phosphate deacetylase from a psychrotrophic marine bacterium, alteromonas species.	a psychrotrophic bacterium, strain mct-9, which produced an n-acetylglucosamine-6-phosphate deacetylase, was isolated from a deep-seawater sample in the mariana trough. the mct-9 strain was identified as alteromonas sp. the native enzyme had a molecular mass of 164,000 da, and was predicted to be composed of four identical subunits with molecular masses of 41,000 da. the purified enzyme hydrolyzed n-acetylglucosamine (glcnac), glcnac-6-phosphate, and glcnac-6-sulfate. considering the low k(m) an ...	2000	10804240
expression, purification, crystallization and preliminary x-ray analysis of the iota-carrageenase from alteromonas fortis.	this is the first crystallization report of a glycoside hydrolase which belongs to family 82. a recombinant form of his-tagged iota-carrageenase from alteromonas fortis was expressed, purified and crystallized. crystals were obtained by the vapour-diffusion method using polyethylene glycol (m(w) = 6000) as a precipitant. they belong to space group p2(1), with unit-cell parameters a = 56. 75, b = 91.04, c = 125.01 a, beta = 93.41 degrees. the unit cell contains two molecules in the asymmetric uni ...	2000	10818359
reclassification of alteromonas distincta romanenko et al. 1995 as pseudoalteromonas distincta comb. nov.	the 16s rrna gene of alteromonas distincta kmm 638t was amplified, cloned and sequenced. the nucleotide sequence was aligned with sequences of representative strains of alteromonas, moritella, pseudoalteromonas and shewanella. results of phylogenetic analysis, using neighbour-joining and fitch-margoliash methods, clearly indicated that this species should be assigned to the genus pseudoalteromonas. on the basis of polyphasic data obtained from previous work and this study, it is proposed that th ...	2000	10826797
assignment of alteromonas elyakovii kmm 162t and five strains isolated from spot-wounded fronds of laminaria japonica to pseudoalteromonas elyakovii comb. nov. and the extended description of the species.	a marine bacterium, alteromonas elyakovii kmm 162t, which was described recently, and five strains isolated from spot-wounded fronds of laminaria japonica have been subjected to phylogenetic analysis, and geno- and phenotypic characterization. the phenotypic features of pseudoalteromonas elyakovii strains were closely related to that of pseudoalteromonas espejiana iam 12640t, but utilization of three carbon compounds (d-mannose, l-tyrosine and trehalose) distinguished both species. the g+c conte ...	2000	10826813
a dna ligase from the psychrophile pseudoalteromonas haloplanktis gives insights into the adaptation of proteins to low temperatures.	the cloning, overexpression and characterization of a cold-adapted dna ligase from the antarctic sea water bacterium pseudoalteromonas haloplanktis are described. protein sequence analysis revealed that the cold-adapted ph dna ligase shows a significant level of sequence similarity to other nad+-dependent dna ligases and contains several previously described sequence motifs. also, a decreased level of arginine and proline residues in ph dna ligase could be involved in the cold-adaptation strateg ...	2000	10848966
substrate and stereochemical specificity of the organophosphorus acid anhydrolase from alteromonas sp. jd6.5 toward p-nitrophenyl phosphotriesters.	the enzyme opaa hydrolyzes p-nitrophenyl phosphotriesters bearing substituents at the phosphorus center ranging in size from methyl to phenyl. the enzyme exhibits stereoselectivity toward the hydrolysis of chiral substrates with a preference for the sp enantiomer.	2000	10866401
culturability and in situ abundance of pelagic bacteria from the north sea.	the culturability of abundant members of the domain bacteria in north sea bacterioplankton was investigated by a combination of various cultivation strategies and cultivation-independent 16s rrna-based techniques. we retrieved 16s rrna gene (rdna) clones from environmental dnas and determined the in situ abundance of different groups and genera by fluorescence in situ hybridization (fish). a culture collection of 145 strains was established by plating on oligotrophic medium. isolates were screen ...	2000	10877804
in vitro studies on sterically stabilized liposomes (sl) as enzyme carriers in organophosphorus (op) antagonism.	this study describes a new approach for organophosphorous (op) antidotal treatment by encapsulating an op hydrolyzing enzyme, opa anhydrolase (opaa), within sterically stabilized liposomes. the recombinant opaa enzyme was derived from alteromonas strain jd6. it has broad substrate specificity to a wide range of op compounds: dfp and the nerve agents, soman and sarin. liposomes encapsulating opaa (sl)* were made by mechanical dispersion method. hydrolysis of dfp by (sl)* was measured by following ...	2000	10892408
differentiation of chitinase-active and non-chitinase-active subpopulations of a marine bacterium during chitin degradation.	the ability of marine bacteria to adhere to detrital particulate organic matter and rapidly switch on metabolic genes in an effort to reproduce is an important response for bacterial survival in the pelagic marine environment. the goal of this investigation was to evaluate the relationship between chitinolytic gene expression and extracellular chitinase activity in individual cells of the marine bacterium pseudoalteromonas sp. strain s91 attached to solid chitin. a green fluorescent protein repo ...	2000	10919822
spatial and temporal variations in chitinolytic gene expression and bacterial biomass production during chitin degradation.	growth of the chitin-degrading marine bacterium s91 on solid surfaces under oligotrophic conditions was accompanied by the displacement of a large fraction of the surface-derived bacterial production into the flowing bulk aqueous phase, irrespective of the value of the surface as a nutrient source. over a 200-h period of surface colonization, 97 and 75% of the bacterial biomass generated on biodegradable chitin and a nonnutritional silicon surface, respectively, detached to become part of the fr ...	2000	10919823
[screening of marine bacteria for fucoidan hydrolases].	twenty-five strains of epiphytic marine bacteria isolated from the brown algae fucus evanescens and chorda filum and fifty-three bacteria isolated from the sea cucumber apostichopus japonicus were screened for fucoidanases using fucoidans prepared from the brown algae f. evanescens, laminaria cichorioides, and l. japonica. eighteen bacterial epiphytes and thirty-eight bacterial isolates from the sea cucumber were found to contain fucoidanases, which were able to hydrolyze either all of the fucoi ...	2000	10920807
iota-carrageenases constitute a novel family of glycoside hydrolases, unrelated to that of kappa-carrageenases.	iota-carrageenases are polysaccharide hydrolases that cleave the beta-1,4 linkages between the d-galactose-4-sulfate and 3, 6-anhydro-d-galactose-2-sulfate residues in the red algal galactans known as iota-carrageenans. we report here on the purification of iota-carrageenase activity from the marine bacterium zobellia galactanovorans and on the characterization of iota-carrageenase structural genes. genomic libraries from this latter bacterium as well as from alteromonas fortis were functionally ...	2000	10934194
the evolutionary history of chromosomal super-integrons provides an ancestry for multiresistant integrons.	integrons are genetic elements that acquire and exchange exogenous dna, known as gene cassettes, by a site-specific recombination mechanism. characterized gene cassettes consist of a target recombination sequence (attc site) usually associated with a single open reading frame coding for an antibiotic resistance determinant. the affiliation of multiresistant integrons (mris), which contain various combinations of antibiotic resistance gene cassettes, with transferable elements underlies the rapid ...	2001	11209061
structure of an acidic polysaccharide from a marine bacterium pseudoalteromonas distincta kmm 638 containing 5-acetamido-3,5,7,9-tetradeoxy-7-formamido-l-glycero-l-manno-nonulosonic acid.	an acidic polysaccharide was obtained from the lipopolysaccharide of pseudoalteromonas distincta strain kmm 638, isolated from a marine sponge, and found to contain d-glca, d-galnac, 2-acetamido-2,6-dideoxy-d-glucose (d-quinac) and two unusual acidic amino sugars: 2-acetamido-2-deoxy-d-galacturonic acid (d-galnaca) and 5-acetamido-3,5,7,9-tetradeoxy-7-formamido-l-glycero-l-manno-nonulosonic acid (pse5ac7fo, a derivative of pseudaminic acid). oligosaccharides were derived from the polysaccharide ...	2001	11217976
phylogenetic analysis of the succession of bacterial communities in the great south bay (long island).	bacterial community composition and succession were examined over the course of the summer season in the great south bay, long island, ny, usa, using a 16s rdna clone library approach. there was a progression of changes in dominant species in the libraries during the summer of 1997. the july library had several groups dominant, the sar407 relatives of the alpha-proteobacteria (24%) and the sar86 (18%), sulfur-oxidizing symbiont relatives (8%) of the gamma-proteobacteria, and unidentified cytopha ...	2001	11248393
purification and characterization of bifunctional alginate lyase from alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates.	a marine bacterium (strain no. 272) isolated from sea mud in omura bay produced an alginate lyase and was classified as an alteromonas species. the enzyme was purified from the culture medium of the bacterium by deae-cellulofine, sephadex g-100 gel chromatography to an electrophoretically homogeneous state in the presence and absence of sds. the molecular mass of the enzyme was 23 and 33.9 kda on sephadex g-100 column chromatography and sds-polyacrylamide gel electrophoresis, respectively, with ...	2001	11272816
stereochemical specificity of organophosphorus acid anhydrolase toward p-nitrophenyl analogs of soman and sarin.	organophosphorus acid anhydrolase (opaa) catalyzes the hydrolysis of p-nitrophenyl analogs of the organophosphonate nerve agents, sarin and soman. the enzyme is stereoselective toward the chiral phosphorus center by displaying a preference for the r(p)-configuration of these analogs. opaa also exhibits an additional preference for the stereochemical configuration at the chiral carbon center of the soman analog. the preferred configuration of the chiral carbon center is dependent upon the configu ...	2001	11300693
[a new family of alteromonadaceae fam. nov., including the marine proteobacteria species alteromonas, pseudoalteromonas, idiomarina i colwellia].	the taxonomic position of the marine genera alteromonas, pseudoalteromonas, idiomarina, and colwellia within the gamma subclass of the class proteobacteria were specified on the basis of their phenotypic, genotypic, and phylogenetic characteristics. gram-negative aerobic bacteria of the genera alteromonas, pseudoalteromonas, and idiomarina and facultatively anaerobic bacteria of the genus colwellia were found to form a phylogenetic cluster with a 16s rrna sequence homology of 90% or higher. the ...	2001	11338830
[tolerance of marine proteobacteria pseudoalteromonas and alteromonas to heavy metals compounds].		2001	11386064
production and separation of alpha-agarase from altermonas agarlyticus strain gj1b.	this paper presents results on the production of alpha-agarase by a fermentation process and its separation using membrane microfiltration (mf). optimization of fermentation conditions for alpha-agarase production using altermonas agarlyticus grown on medium containing agar as a carbon source was done in batch, fed-batch and continuous fermentations. continuous culture at a dilution rate of 0.03 h(-1) appeared to be best suited for production of alpha-agarase by this organism. at 0.03 h(-1) dilu ...	2001	11396907
characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16s rdna sequences.	one hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophylic conditions from arctic (98 strains) and antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. by numerical analysis, 8 clusters, containing 2 to 59 strains, could be delineated, and 8 strains formed separate branches. five clusters contained strains from both poles, two minor clusters were confined to arctic ...	2001	11403404
retrieval of the species alteromonas tetraodonis simidu et al. 1990 as pseudoalteromonas tetraodonis comb. nov. and emendation of description.	a polyphasic taxonomy study was undertaken of three strains of pseudoalteromonas haloplanktis subsp. tetraodonis (simidu et al. 1990) gauthier et al. 1995. dna was prepared from each of the strains and genomic relatedness was measured by dna-dna hybridization. strains kmm 458t and iam 14160t shared 99% genetic relatedness, but were only 48-49% related to the type strain of pseudoalteromonas haloplanktis subsp. haloplanktis, iam 12915t. the third strain, p. haloplanktis subsp. tetraodonis a-m, sh ...	2001	11411675
the kappa-carrageenase of p. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of clan-b glycoside hydrolases.	kappa-carrageenans are gel-forming, sulfated 1,3-alpha-1,4-beta-galactans from the cell walls of marine red algae. the kappa-carrageenase from the marine, gram-negative bacterium pseudoalteromonas carrageenovora degrades kappa-carrageenan both in solution and in solid state by an endoprocessive mechanism. this beta-galactanase belongs to the clan-b of glycoside hydrolases.	2001	11435116
strong ligands for thorium complexation in marine bacteria.	the interaction between thorium and marine organisms (cultured heterotrophic bacteria) was experimentally examined by using chemical equilibrium techniques. thorium (th) quantitatively reacts with a binding site on bacteria (alteromonas, vibrio, pseudomonas and flavobacterium) in 0.1 m hcl solution. according to mass balance analysis of adsorption experiment data, th forms a 1:1 complex with a binding site similar in reactivity among bacteria used in this study, whose conditional stability const ...	2001	11468816
validation of publication of new names and new combinations previously effectively published outside the ijsem.		2001	11491317
the iota-carrageenase of alteromonas fortis. a beta-helix fold-containing enzyme for the degradation of a highly polyanionic polysaccharide.	carrageenans are gel-forming hydrocolloids extracted from the cell walls of marine red algae. they consist of d-galactose residues bound by alternate alpha(1-->3) and beta(1-->4) linkages and substituted by one (kappa-carrageenan), two (iota-carrageenan), or three (lambda-carrageenan) sulfate-ester groups per disaccharide repeating unit. both the kappa- and iota-carrageenan chains adopt ordered conformations leading to the formation of highly ordered aggregates of double-stranded helices. severa ...	2001	11493601
o-specific polysaccharide of the marine bacterium "alteromonas marinoglutinosa" ncimb 1770.	the o-specific polysaccharide of the marine bacterium "alteromonas marinoglutinosa" ncimb 1770 was obtained by mild acid degradation of the corresponding lipopolysaccharide and found to contain d-galactose, n-acetyl-d-glucosamine, and n-acetyl-d-mannosamine residues in equimolar ratio. based on methylation analysis, periodate oxidation, and 13c-nmr spectroscopy data of native and modified polysaccharides, the following structure of the trisaccharide repeating unit of the o-specific polysaccharid ...	2001	11566059
structural investigation on the lipooligosaccharide fraction of psychrophilic pseudoalteromonas haloplanktis tac 125 bacterium.	the core structure of the cell-wall lipooligosaccharide (los) fraction of an antarctic gram-negative bacterium, pseudoalteromonas haloplanktis tac 125 strain, was determined to be deacetylated alditols. these were obtained from native los fraction by o-deacylation, dephosphorylation, reduction and finally n-deacylation. two novel structures were detected, the more highly represented molecule consisting of the following hexasaccharide chain: alpha-d-manpnh(2)-(1-->3)-beta-d-galp-(1-->4)-alpha-l-g ...	2001	11589700
characterization, chemical modifications and in vitro anticoagulant properties of an exopolysaccharide produced by alteromonas infernus.	a new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. two depolymerization processes were used to obtain low-molecular-weight (24-35x10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%). nuclear magnetic resonance studies indicate ...	2001	11687301
[gram-negative eubacteria isolated from the water, molluscs and algae of the black sea].	microorganisms strains (384) have been isolated from water, algae and molluscs collected in the karadag reserve and the village of katsiveli (black sea shore of the crimea); 81 of the above strains are gram-negative oxidase-positive rods which require sodium ions for their growth. a group of nonfermenting mobile bacteria deprived of arginine dihydrolase have been distinguished among the latter; as to their physiological and biochemical properties and sensitivity to some antibiotics these bacteri ...	2001	11692675
chemical characterization of lipid a from some marine proteobacteria.	lipids a from type and wild strains of marine proteobacteria belonging to alteromonadaceae (alteromonas (1 species), idiomarina (1 species), and pseudoalteromonas (8 species) genera) and vibrionaceae (shewanella (1 species) and vibrio (1 species) genera) families and marinomonas genus (1 species) were isolated by hydrolysis of their respective lipopolysaccharides with 1% acetic acid. based on thin-layer chromatography data, the lipids a studied had low heterogeneity and generated family-specific ...	2001	11703190
microbial communities in the chemocline of a hypersaline deep-sea basin (urania basin, mediterranean sea).	the urania basin is a hypersaline sulfidic brine lake at the bottom of the eastern mediterranean sea. since this basin is located at a depth of approximately 3,500 m below the sea surface, it receives only a small amount of phytoplankton organic carbon. in the present study, the bacterial assemblages at the interface between the hypersaline brine and the overlaying seawater were investigated. the sulfide concentration increased from 0 to 10 mm within a vertical interval of 5 m across the interfa ...	2001	11722884
microbial diversity in smoked salmon examined by a culture-independent molecular approach--a preliminary study.	microbial biodiversity in sliced vacuum-packed cold smoked salmon was investigated using culture-independent molecular biology techniques. sliced smoked salmon was stored for 25 days after being packed at 4 degrees c. dna was extracted from sliced vacuum-packed cold smoked salmon. pcr dna amplification were carried out using universal eubacterial primers corresponding to escherichia coli 16s rrna gene. 16s rrna genes were amplified, cloned in e. coli and compared using amplification ribosomal dn ...	2001	11759756
[the new biocontrol strategy of algae pollution].		2001	12549097
identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, alteromonas sp. strain o-7.	alteromonas sp. strain o-7 secretes chitinase a (chia), chitinase b (chib), and chitinase c (chic) in the presence of chitin. a gene cluster involved in the chitinolytic system of the strain was cloned and sequenced upstream of and including the chia gene. the gene cluster consisted of three different open reading frames organized in the order chid, cbp1, and chia. the chid, cbp1, and chia genes were closely linked and transcribed in the same direction. sequence analysis indicated that cbp1 (475 ...	2002	11772635
phylogenetic composition of bacterioplankton assemblages from the arctic ocean.	we analyzed the phylogenetic composition of bacterioplankton assemblages in 11 arctic ocean samples collected over three seasons (winter-spring 1995, summer 1996, and summer-fall 1997) by sequencing cloned fragments of 16s rrna genes. the sequencing effort was directed by denaturing gradient gel electrophoresis (dgge) screening of samples and the clone libraries. sequences of 88 clones fell into seven major lineages of the domain bacteria: alpha(36%)-, gamma(32%)-, delta(14%)-, and epsilon(1%)-p ...	2002	11823184
molecular analysis of the gene encoding a novel chitin-binding protease from alteromonas sp. strain o-7 and its role in the chitinolytic system.	alteromonas sp. strain o-7 secretes several proteins in response to chitin induction. we have found that one of these proteins, designated apriv, is a novel chitin-binding protease involved in chitinolytic activity. the gene encoding apriv (apriv) was cloned in escherichia coli. dna sequencing analysis revealed that the open reading frame of apriv encoded a protein of 547 amino acids with a calculated molecular mass of 57,104 da. apriv is a modular enzyme consisting of five domains: the signal s ...	2002	11889092
halogenated metabolites from the new okinawan red alga laurencia yonaguniensis.	a novel brominated diterpene based on the rare neoirieane skeleton, named neoirietetraol (1), has been isolated along with a halogenated c15 acetogenin, (3z)-laurenyne (2), from a new laurencia species, l.yonaguniensis masuda et abe, species inedita, collected at yonaguni island, okinawa prefecture, japan. the structures of these metabolites were elucidated by spectroscopic data (ir, 1h nmr, 13c nmr, 2d nmr, and ms). neoirietetraol (1) was toxic to the brine shrimp (altemia salina; lc50, 40.1 mi ...	2002	11908989
prevalence and microdiversity of alteromonas macleodii-like microorganisms in different oceanic regions.	the presence, prevalence and variability of microorganisms related to the species alteromonas macleodii, a well known culturable gamma-proteobacterium, has been studied in different seawater samples from diverse geographical locations, in both the northern and southern hemispheres, and tested with two molecular techniques (rrna hybridization and gene cloning and sequencing). results show that a. macleodii-like microorganisms are present in high proportions in north atlantic and, especially, medi ...	2002	11966824
stimulation of alexandrium fundyense growth by bacterial assemblages from the bay of fundy.	to evaluate the influence of marine bacterial assemblages from the bay of fundy on alexandrium fundyense str. cb301 growth.	2002	11966911
extracellular polysaccharides of rhodococcus rhodochrous s-2 stimulate the degradation of aromatic components in crude oil by indigenous marine bacteria.	rhodococcus rhodochrous s-2 produces extracellular polysaccharides (s-2 eps) containing d-glucose, d-galactose, d-mannose, d-glucuronic acid, and lipids, which is important to the tolerance of this strain to an aromatic fraction of (af) arabian light crude oil (n. iwabuchi, n. sunairi, h. anzai, m. nakajima, and s. harayama, appl. environ. microbiol. 66:5073-5077, 2000). in the present study, we examined the effects of s-2 eps on the growth of indigenous marine bacteria on af. indigenous bacteri ...	2002	11976106
bacterial distribution and phylogenetic diversity in the changjiang estuary before the construction of the three gorges dam.	the bacterial community structure in the changjiang estuary was studied for comparison with future changes, related to the construction of the three gorges dam. population densities of bacteria in the surface water at station c1 estimated by cfu on marine agar plates and by dapi direct count, were 2.8 x 10(4) ml(-1) and 4.2 x 10(5) ml(-1), respectively. physicochemical properties of water, such as temperature and salinity, suggested that station c1 was affected by freshwater from the changjiang ...	2002	11984631
isolation and characterization of the genes encoding two metalloproteases (mpri and mprii) from a marine bacterium, alteromonas sp. strain o-7.	an extracellular alkaline metalloprotease (mpri) from alteromonas sp. strain o-7 was purified and characterized. the molecular mass of the purified enzyme was estimated to be 56 kda by sds-page. the optimum ph and temperature were ph 10.0 and 60 degrees c, respectively. the gene (mpri) encoding mpri was cloned and its nucleotide sequence was analyzed. the deduced amino acid sequence of mpri showed significant similarity to metalloproteases classified into the thermolysin family. furthermore, seq ...	2002	11999419
molecular cloning of the gene encoding a novel beta-n-acetylhexosaminidase from a marine bacterium, alteromonas sp. strain o-7, and characterization of the cloned enzyme.	we have reported that the chitinolytic system of alteromonas sp. strain o-7 consists of chitinases (chia, chib, and chic), a chitinase-like enzyme (chid), beta-n-acetylglucosaminidases (glcnacasesa, glcnacaseb, and glcnacasec), and a novel transglycosylative enzyme (hex99). the gene encoding a beta-hexosaminidase with an unusual substrate specificity (hex86), located upstream of the hex99 gene, was cloned and sequenced. the gene encoded a protein of 761 amino acids with a calculated molecular ma ...	2002	11999431
phylogenetic diversity of numerically important arctic sea-ice bacteria cultured at subzero temperature.	heterotrophic bacteria in sea ice play a key role in carbon cycling, but little is known about the predominant players at the phylogenetic level. in a study of both algal bands and clear ice habitats within summertime arctic pack ice from the chukchi sea, we determined the abundance of total bacteria and actively respiring cells in melted ice samples using epifluorescence microscopy and the stains 4', 6'-diamidino-2-phenylindole 2hcl (dapi) and 5-cyano-2,3-ditolyl tetrazolium chloride (ctc), res ...	2002	12037610
bisucaberin--a dihydroxamate siderophore isolated from vibrio salmonicida, an important pathogen of farmed atlantic salmon (salmo salar).	a siderophore of the bacterial fish pathogen, vibrio salmonicida, was isolated from low-iron culture supernatant and structurally characterized as bisucaberin by fticr- and fab-ms, nmr and gc-ms analysis of the hydrolysis products. although the cyclic dihydroxamate bisucaberin has previously been isolated from a marine bacterium, alteromonas haloplanktis, its involvement in cold-water vibriosis of atlantic salmon (salmon salar) is novel. bisucaberin production in iron-limited media was highest a ...	2002	12046923
use of pcr-rflp for genotyping 16s rrna and characterizing bacteria cultured from halibut fry.	small subunit ribosomal genes were explored using pcr-rflp to facilitate the characterization of bacteria cultured from reared fry of the atlantic halibut (hippoglossus hippoglossus). concern has been expressed about pathogen invasion in larvae lacking a counteracting normal flora that may aid the immune system in producing robust noninfected individuals. in this study, pure cultured representatives of normal flora that were previously found to be antagonistic towards a pathogenic vibrio sp. wer ...	2002	12109877
structure of o-specific polysaccharide from pseudoalteromonas nigrifaciens strain kmm 161.	on mild acid degradation of the lipopolysaccharide of the marine microorganism pseudoalteromonas nigrifaciens kmm 161 an o-specific polysaccharide containing d-galactose, 2-acetamido-2-deoxy-d-glucose, 3,6-dideoxy-3-(4-hydroxybutyramido)-d-galactose, and 2-acetamido-2-deoxy-l-guluronic acid residues was obtained. from the results of smith degradation, o-deacetylation of the polysaccharide, and nmr spectroscopy the following structure of the tetrasaccharide repeating unit of the o-specific polysa ...	2002	12126475
a novel polymer produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid.	the objective of the present work was to describe an aerobic, mesophilic and heterotrophic marine bacterium, designated hyd657, able to produce an exopolysaccharide (eps). it was isolated from a east pacific rise deep-sea hydrothermal vent polychaete annelid.	2002	12147080
[antibiotic properties of the pseudoalteromonas genus bacteria isolated from the black sea water and molluscs].	antagonistic properties of 41 strains of alteromonas-like bacteria isolated from the black sea water and molluscs have been studied. being grown on the rich medium "b" for marine bacteria, 21% of strains have shown high antagonistic activity against phytopathogenic fungi; 6% of strains inhibited the growth of bacillus subtilis, proteus vulgaris and candida albicans. spectrum of antagonistic activity was essentially changed on synthetic "bm" medium with acetate, glutamate, alpha-alanine as a sing ...	2002	12190034
a metalloprotease (mpriii) involved in the chitinolytic system of a marine bacterium, alteromonas sp. strain o-7.	alteromonas sp. strain o-7 secretes several proteins in addition to chitinolytic enzymes in response to chitin induction. in this paper, we report that one of these proteins, designated mpriii, is a metalloprotease involved in the chitin degradation system of the strain. the gene encoding mpriii was cloned in escherichia coli. the open reading frame of mpriii encoded a protein of 1,225 amino acids with a calculated molecular mass of 137,016 da. analysis of the deduced amino acid sequence of mpri ...	2002	12406750
identification of dna-synthesizing bacterial cells in coastal north sea plankton.	we describe a method for microscopic identification of dna-synthesizing cells in bacterioplankton samples. after incubation with the halogenated thymidine analogue bromodeoxyuridine (brdu), environmental bacteria were identified by fluorescence in situ hybridization (fish) with horseradish peroxidase (hrp)-linked oligonucleotide probes. tyramide signal amplification was used to preserve the fish staining during the subsequent immunocytochemical detection of brdu incorporation. dna-synthesizing c ...	2002	12406771
a preliminary report of phylogenetic diversity of bacterial strains isolated from marine creatures.	bacterial diversity among marine creatures, especially molluscs, as a source for searching out novel lineages of bacteria, was studied. marine creatures were collected at the coasts of the kanto area in japan. a total of 116 strains of bacteria were isolated from the intestines of 19 species of marine creatures includings molluscs, pisces and protochordata. partial sequencing of 16s rdna revealed that most of the isolates belonged to the gamma subclass of the proteobacteria and cytophaga-flavoba ...	2002	12501435
diversity of 746 heterotrophic bacteria isolated from microbial mats from ten antarctic lakes.	microbial mats, growing in antarctic lakes constitute unique and very diverse habitats. in these mats microorganisms are confronted with extreme life conditions. we isolated 746 bacterial strains from mats collected from ten lakes in the dry valleys (lakes hoare and fryxell), the vestfold hills (lakes ace, druzhby, grace, highway, pendant, organic and watts) and the larsemann hills (lake reid), using heterotrophic growth conditions. these strains were investigated by fatty acid analysis, and by ...	2002	12583721
single-strand-specific nucleases.	single-strand-specific nucleases are multifunctional enzymes and widespread in distribution. their ability to act selectively on single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids has led to their extensive application as probes for the structural determination of nucleic acids. intracellularly, they have been implicated in recombination, repair and replication, whereas extracellular enzymes have a role in nutrition. although more than 30 single-strand-spe ...	2003	12586391
biodegradation of toxic chemicals in guayanilla bay, puerto rico.	studies were conducted to assess the factors that may influence the rate and extent of biodegradation of biphenyl, naphthalene, phenanthrene, pentachlorophenol (pcp) and p-nitrophenol in water samples collected from the guayanilla bay (18 degrees n; 67.45 degrees w), southwest of puerto rico. in vitro studies mediated slow degradation of biphenyl, naphthalene and phenanthrene substrates by natural microbial flora present in the bay. addition of kno(3) as a source of inorganic n greatly enhanced ...	2003	12705914
enzymatically depolymerized alginate oligomers that cause cytotoxic cytokine production in human mononuclear cells.	enzymatically depolymerized guluronate and mannuronate oligomers were prepared from polyuronates with an alginate lyase from a pseudoalteromonas sp., and their effects on mononuclear cells from human peripheral blood were examined. conditioned medium prepared by the incubation of cells with an untreated polyuronate had little effect on growth of human leukemic u937 cells, but a medium prepared with depolymerized uronate oligomers inhibited their growth. inhibition was greater in a medium prepare ...	2003	12728983
are readily culturable bacteria in coastal north sea waters suppressed by selective grazing mortality?	we studied the growth of six culturable bacterial lineages from coastal north sea picoplankton in environmental samples under different incubation conditions. the grazing pressure of heterotrophic nanoflagellates (hnf) was reduced either by double prefiltration through 0.8- micro m-pore-size filters or by 10-fold dilutions with 0.2- micro m (pore-size) prefiltered seawater. we hypothesized that those gamma-proteobacterial genera that are rapidly enriched would also be most strongly affected by h ...	2003	12732530
a novel, highly viscous polysaccharide excreted by an alteromonas isolated from a deep-sea hydrothermal vent shrimp.	a deep-sea, mesophilic, aerobic, and heterotrophic microorganism, able to produce an extracellular polysaccharide, was isolated from a shrimp collected near an active hydrothermal vent of the mid-atlantic ridge. on the basis of phenotypic and phylogenetic analyses and dna/dna relatedness, this strain could be assigned to the species alteromonas macleodii as a variant of the fijiensis subspecies. it was selected for its ability to exhibit a swarming mucoid phenotype on specific media. the bacteri ...	2003	12732953
glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium.	alteromonas-like strains kmm 241(t) and kmm 642, isolated from marine invertebrate specimens, were investigated to clarify their taxonomic position. the novel isolates were aerobic, gram-negative, motile, slightly halophilic and heterotrophic and hydrolysed polysaccharides. they did not hydrolyse urea, gelatin or casein and produced acid weakly from carbohydrates. the dna g + c content ranged between 44.6 and 44.8 mol%. dna-dna similarity between the two strains was 71%. comparison of the 16s rr ...	2003	12807181
degradation of chlorinated biphenyl, dibenzofuran, and dibenzo-p-dioxin by marine bacteria that degrade biphenyl, carbazole, or dibenzofuran.	marine bacterial strains (bp-ph, car-sf, and dbf-mak) were isolated using biphenyl, carbazole (car), or dibenzofuran (df) respectively as substrates for growth. their 16s ribosomal dna sequences showed that the species closest to strain bp-ph, strain car-sf, and strain dbf-mak are alteromonas macleodii (96.3% identity), neptunomonas naphthovorans (93.1% identity), and cycloclasticus pugetii (97.3% identity), respectively. the metabolites produced suggested that strain car-sf degrades car via dio ...	2003	12834291
tolerance to various toxicants by marine bacteria highly resistant to mercury.	bacteria highly resistant to mercury isolated from seawater and sediment samples were tested for growth in the presence of different heavy metals, pesticides, phenol, formaldehyde, formic acid, and trichloroethane to investigate their potential for growth in the presence of a variety of toxic xenobiotics. we hypothesized that bacteria resistant to high concentrations of mercury would have potential capacities to tolerate or possibly degrade a variety of toxic materials and thus would be importan ...	2003	12876655
assignment of 'alteromonas marinoglutinosa' ncimb 1770 to pseudoalteromonas mariniglutinosa sp. nov., nom. rev., comb. nov.	the taxonomic position of the marine bacterium 'alteromonas marinoglutinosa' ncimb 1770 was investigated in a polyphasic study. analysis of 16s rdna sequence and dna-dna reassociation values confirmed the phylogenetic position of strain ncimb 1770 within the genus pseudoalteromonas as a separate species, distinct from all pseudoalteromonas species with validly described names. on the basis of physiological and molecular properties, it is proposed that strain ncimb 1770 is classified as pseudoalt ...	2003	12892134
characterization of a chitin-binding protein gp37 of spodoptera litura multicapsid nucleopolyhedrovirus.	the gp37 amino acid sequence of spodoptera litura multicapsid nucleopolyhedrovirus (spltmnpv) was compared with other baculovirus gp37, entomopoxvirus fusolin, the enhancing factor of pseudaletia separata entomopoxvirus, and alteromonas sp. chitin-binding protein 1. in these proteins, five 'conserved regions' previously reported constitute a chitin-binding domain. spltmnpv gp37 effectively bound to purified crab shell chitin and the dissociation constant (kd) for binding was 0.28 microm. immunof ...	2003	12951271
similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates.	tetracycline-resistant (tet(r)) bacteria were isolated from fishes collected at three different fish farms in the southern part of japan in august and september 2000. of the 66 tet(r) gram-negative strains, 29 were identified as carrying tetb only. four carried tety, and another four carried tetd. three strains carried tetc, two strains carried tetb and tety, and one strain carried tetc and tetg. sequence analyses indicated the identity in tet(r) genes between the fish farm bacteria and clinical ...	2003	12957921
hepatic cholangiocarcinoma and testicular mesothelioma in a wild-caught blue shark, prionace glauca (l.).	an adult male blue shark, prionace glauca (l.), caught in july 2000 by a recreational fisherman off long island, new york, usa, had a retained fishing hook from a previous capture. the hook penetrated the gastric wall and lacerated the right liver lobe. macroscopic lesions consisted of transmural gastritis and peritonitis. alteromonas sp. and vibrio alginolyticus were isolated from the peritoneal fluid. in addition, a well delineated, sessile mass was found on the otherwise normal serosa of the ...	2003	12962210
alteromonas marina sp. nov., isolated from sea water of the east sea in korea.	two gram-negative, motile, non-spore-forming and moderately halophilic rods (strains sw-47(t) and sw-49) were isolated from sea water of the east sea in korea and subjected to a polyphasic taxonomic study. the two strains grew optimally between 30 and 37 degrees c, and grew at 4 and 44 degrees c but not at temperatures above 45 degrees c. they grew optimally in the presence of 2-5 % (w/v) nacl, but did not grow in the absence of nacl. strains sw-47(t) and sw-49 had ubiquinone-8 (q-8) as the pred ...	2003	13130060
culturable and nonculturable bacterial symbionts in the toxic benthic dinoflagellate ostreopsis lenticularis.	the toxic benthic dinoflagellate ostreopsis lenticularis hosts a variety of symbiont bacterial flora. laboratory cultured ostreopsis clones require the presence of symbiotic pseudomonas/alteromonas bacterial strains for growth and toxicity development. three culturable bacterial strains associated with ostreopsis were identified as pseudomonas/alteromonas strain 1, pseudomonas/alteromonas strain 2 and acinetobacter. denaturing gradient gel electrophoresis (dgge) analyses of extracted ostreopsis ...	2003	14505943
the structural bases of the processive degradation of iota-carrageenan, a main cell wall polysaccharide of red algae.	iota-carrageenans are sulfated 1,3-alpha-1,4-beta-galactans from the cell walls of red algae, which auto-associate into crystalline fibers made of aggregates of double-stranded helices. iota-carrageenases, which constitute family 82 of glycoside hydrolases, fold into a right-handed beta-helix. here, the structure of alteromonas fortis iota-carrageenase bound to iota-carrageenan fragments was solved at 2.0a resolution (pdb 1ktw). the enzyme holds a iota-carrageenan tetrasaccharide (subsites +1 to ...	2003	14623184
differential growth response of colony-forming alpha- and gamma-proteobacteria in dilution culture and nutrient addition experiments from lake kinneret (israel), the eastern mediterranean sea, and the gulf of eilat.	even though it is widely accepted that bacterioplankton growth in lakes and marine ecosystems is determined by the trophic status of the systems, knowledge of the relationship between nutrient concentrations and growth of particular bacterial species is almost nonexistent. to address this question, we performed a series of culture experiments with water from lake kinneret (israel), the eastern mediterranean sea, and the gulf of eilat (northern red sea). in the initial water samples, the proporti ...	2003	12513996
2-n-pentyl-4-quinolinol produced by a marine alteromonas sp. and its potential ecological and biogeochemical roles.	bacterium-bacterium interactions occur at intimate spatial scales on the order of micrometers, but our knowledge of interactions at this level is rudimentary. antagonism is a potential interaction in such microenvironments. to study the ecological role of antibiosis, we developed a model system involving an antibiotic-producing isolate (swat5) derived from a marine particle and its dominant antibiotic product, 2-n-pentyl-4-quinolinol (pq). this system was used to address questions about the sign ...	2003	12514043
molecular analysis of the gene encoding a novel cold-adapted chitinase (chib) from a marine bacterium, alteromonas sp. strain o-7.	the chitinase b (chib) secreted by alteromonas sp. strain o-7 was purified, and the corresponding gene (chib) was cloned and sequenced. the open reading frame of the chib gene encodes a protein of 850 amino acids with a calculated molecular mass of 90,223 da. chib is a modular enzyme consisting of two reiterated domains and a catalytic domain belonging to chitinase family 18. the reiterated domains are composed of chitin-binding domain (chtbd) type 3 and two fibronectin type iii (fn3)-like domai ...	2003	12562783
gtx(4) imposters: characterization of fluorescent compounds synthesized by pseudomonas stutzeri sf/ps and pseudomonas/alteromonas ptb-1, symbionts of saxitoxin-producing alexandrium spp.	saxitoxins, the etiological agent of paralytic shellfish poisoning, are synthesized by dinoflagellates and cyanobacteria. several reports indicate that bacteria are capable of saxitoxin synthesis. two bacterial strains were isolated from saxitoxin-producing dinoflagellates, alexandrium tamarense and a. lusitanicum (=alexandrium minutum), and grown under a variety of culture conditions including those previously reported to induce saxitoxin synthesis in bacteria. five fluorescent compounds were a ...	2003	12565757
from extreme environments to biologically active exopolysaccharides.	in the course of the discovery of novel polysaccharides of biotechnological interest, it is now widely accepted that extremophilic microorganisms will provide a valuable resource not only for exploitation in novel biotechnological processes but also as models for investigating how biomolecules are stabilized when subjected to extreme conditions. microbes isolated from extreme environments offer a great diversity in chemical and physical properties of their eps as compared to anywhere else in the ...	2003	15296169
polymer film produced by a marine bacterium.	biojelly is a sort of polymer that is formed on a cellulose acetate membrane immersed in seawater. interestingly, biojelly inhibits attachment of marine organisms such as algae and barnacles. we could successfully isolate several marine microorganisms from biojelly-attached microorganisms. one of these isolates, strain shy1-1, produced water-insoluble polymeric materials in natural seawater supplemented with yeast extracts and glucose. this strain was assigned to be alteromonas sp. by the method ...	2003	16233369
[a primary study on antimicrobial and cytotoxic activity of marine bacteria].	marine bacteria were isolated from marine organisms including invertebrates and seaweeds and from sea water and sediments of different sea area. ethyl acetate extractions of marine bacterial fermentation were screened for antimicrobial and cytotoxicity activities by using the methods of agar diffusion and mtt assay, and the relationship of active bacteria and their origin was studied. the results showed that 42 strains among total 341 isolated marine bacteria had antimicrobial activity, and 7 st ...	2004	15669499
structural studies of the main exopolysaccharide produced by the deep-sea bacterium alteromonas infernus.	the structure of the extracellular polysaccharide produced by the mesophilic species, alteromonas infernus, found in deep-sea hydrothermal vents and grown under laboratory conditions, has been investigated using partial depolymerization, methylation analysis, mass spectrometry and nmr spectroscopy. the repeating units of this polysaccharide is a nonasaccharide with the following structure: [carbohydrate: see text].	2004	15388352
phylogenetic relationships among marine alteromonas-like proteobacteria: emended description of the family alteromonadaceae and proposal of pseudoalteromonadaceae fam. nov., colwelliaceae fam. nov., shewanellaceae fam. nov., moritellaceae fam. nov., ferrimonadaceae fam. nov., idiomarinaceae fam. nov. and psychromonadaceae fam. nov.	the phylogenetic relationships among marine alteromonas-like bacteria of the genera alteromonas, pseudoalteromonas, glaciecola, thalassomonas, colwellia, idiomarina, oceanimonas, oceanisphaera, shewanella, moritella, ferrimonas, psychromonas and several other genera of the 'gammaproteobacteria' were studied. results of 16s rrna gene sequence analyses revealed that some members of these genera formed several coherent groups at the family level. characteristic signature oligonucleotides for studie ...	2004	15388743
probiotic and nutritional effects of microbial communities on axenic cultures of a rotifer strain brachionus plicatilis.		2004	15560221
rapid screening and dereplication of bacterial isolates from marine sponges of the sula ridge by intact-cell-maldi-tof mass spectrometry (icm-ms).	rapid grouping of bacterial isolates is critical in comprehensive microbial studies of environmental samples or screening programmes e.g. in unknown marine environments where large numbers of strains have to be isolated on different growth media. sets of bacteria have been cultured from the marine sponges isops phlegraei, haliclona sp. 1, phakellia ventilabrum and plakortis sp. growing at a depth of about 300 m on the sula ridge close to the norwegian coast. we employed intact-cell maldi-tof (ic ...	2004	15614563
[characterization of microbial population present in the edible seaweed, monostroma undulatum, wittrock].	the microbiological quality of monostroma undulatum, wittrock from the southern argentinean coast, was studied for its application for human food. also the diversity and function of the native bacterial population to this green seaweed was analyzed. samples were collected in puerto deseado, province of santa cruz, southern argentina (47 degrees 45'l.s., 65 degrees 55'l.w). the samples were analyzed for the presence of psycotrophic heterotrophic bacteria, marine heterotrophic bacteria, low nutrit ...	2004	15807211
aestuariibacter salexigens gen. nov., sp. nov. and aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of alteromonas macleodii.	two strictly aerobic, halophilic strains of the gamma-proteobacteria, designated jc2042(t) and jc2043(t), were obtained from a sediment sample of getbol, the korean tidal flat. comparative 16s rdna sequence studies revealed that the test strains were related most closely to the type strains of the genera alteromonas (93.5-95.5 %) and glaciecola (91.1-93.3 %). phylogenetic analyses demonstrated that strains jc2042(t) and jc2043(t) formed a distinct monophyletic clade within the family alteromonad ...	2004	15023977
microtiter assay for glutamine synthetase biosynthetic activity using inorganic phosphate detection.	a microtiter assay was developed for the improved detection of inorganic phosphate released from adenosine 5'-triphosphate (atp) in the glutamine synthetase biosynthetic assay. in this assay, ascorbic acid replaces the traditionally used ferrous sulfate to reduce the phosphomolybdate complex. as a result, increased color development, linearity, and sensitivity are achieved. additionally, in the microtiter format, multiple sets of kinetic experiments can be rapidly performed in parallel. the colo ...	2004	15033518
[microbial polysaccharides of marine origin and their potential in human therapeutics].	bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. although many known marine bacteria produce exopolysaccharides (eps), continuation in looking for new polysaccharide-producing micro-organisms is promising. marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual eps. with the aim of discovering biological activities, eps presenting different structural features were stu ...	2004	15063931
agarivorans albus gen. nov., sp. nov., a gamma-proteobacterium isolated from marine animals.	six bacterial strains were isolated from healthy marine organisms that were collected from the coast of the kanto area in japan. phylogenetic analysis based on 16s rdna sequence similarity showed that the six isolates formed a separate cluster in the gamma-proteobacteria and were related to the genera alteromonas and glaciecola (<91.6 % similarity). the isolates were related closely to each other (dna-dna reassociation values of 74-93 %). the isolates had a polar flagellum and were gram-negative ...	2004	15143010
low-molecular-weight, biologically active compounds from marine pseudoalteromonas species.	we have examined the ability of marine proteobacteria from the pseudoalteromonas genus and alteromonas macleodii to produce low-molecular-weight, biologically active compounds with antimicrobial and surface-active properties. a new marine bacterium, pseudoalteromonas issachenkonii, exhibited a high level of biological activity and produced antifungal and hemolytic compounds. a detailed spectroscopic investigation based on uv, ir, high-resolution mass spectrometry, and 2d 1h and 13c nuclear magne ...	2004	15170241
recombinant protein production in antarctic gram-negative bacteria.	this review reports some results from our laboratory on the setting up of a psychrophilic expression system for the homologous/heterologous protein production in cold-adapted bacteria by using natural plasmids as cloning vectors. by screening some antarctic bacteria for the presence of extrachromosomal elements, we identified three new plasmids, pmtbl from pseudoalteromonas haloplanktis tac125, and ptaup and ptadw, from psychrobacter sp. ta144. the latter autoreplicating elements were isolated, ...	2004	15269427
alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from antarctic seas, and emended description of the genus alteromonas.	seven novel, cold-adapted, strictly aerobic, facultatively oligotrophic strains, isolated from antarctic sea water, were investigated by using a polyphasic taxonomic approach. the isolates were gram-negative, chemoheterotrophic, motile, rod-shaped cells that were psychrotolerant and moderately halophilic. buds were produced on mother and daughter cells and on prosthecae. prostheca formation was peritrichous and prosthecae could be branched. phylogenetic analysis based on 16s rrna gene sequences ...	2004	15280285
alteromonas litorea sp. nov., a slightly halophilic bacterium isolated from an intertidal sediment of the yellow sea in korea.	a gram-negative, motile, non-spore-forming, rod-shaped bacterium, designated strain tf-22t, was isolated from an intertidal sediment in korea. this organism grew optimally at 30-37 degrees c and in the presence of 2-5% (w/v) nacl. it did not grow without nacl or in the presence of more than 14% (w/v) nacl. strain tf-22t was characterized chemotaxonomically as having ubiquinone-8 as the predominant respiratory lipoquinone and c(16 : 0), c(16 : 1) omega7c and/or iso-c(15 : 0) 2-oh and c(18 : 1) om ...	2004	15280291
incorporation of glucose under anoxic conditions by bacterioplankton from coastal north sea surface waters.	it has been hypothesized that the potential for anaerobic metabolism might be a common feature of bacteria in coastal marine waters (l. riemann and f. azam, appl. environ. microbiol. 68: 5554-5562, 2002). therefore, we investigated whether different phylogenetic groups of heterotrophic picoplankton from the coastal north sea were able to take up a simple carbon source under anoxic conditions. oxic and anoxic incubations (4 h) or enrichments (24 h) of seawater with radiolabeled glucose were perfo ...	2005	15811993
roles of four chitinases (chia, chib, chic, and chid) in the chitin degradation system of marine bacterium alteromonas sp. strain o-7.	alteromonas sp. strain o-7 secretes four chitinases (chia, chib, chic, and chid) in the presence of chitin. to elucidate why the strain produces multiple chitinases, we studied the expression levels of the four genes and proteins, their enzymatic properties, and their synergistic effects on chitin degradation. among the four chitinases, chia was produced in the largest quantities, followed by chid, and the production of chib and chic changed at lower levels than those of chia and chid. the expre ...	2005	15812005
genetic analysis of housekeeping genes reveals a deep-sea ecotype of alteromonas macleodii in the mediterranean sea.	the genetic diversity of 19 strains belonging to alteromonas macleodii isolated from different geographic areas (pacific and indian ocean, and different parts of the mediterranean sea) and at different depths (from the surface down to 3500 m) has been studied. fragments of the 16s rrna gene, the internal transcribed spacer (its) between 16s and 23s rdna genes, the gyrb and the rpob genes, have been sequenced for each strain. amplified fragment length polymorphisms were used to characterize simil ...	2005	15819847
alteromonas addita sp. nov.	on the basis of phenotypic, genotypic characteristics and analysis of 16s rrna gene sequences, a novel species belonging to the genus alteromonas is described. a non-pigmented, motile, gram-negative bacterium designated r10sw13(t) was isolated from sea water samples collected in chazhma bay (sea of japan, pacific ocean). the novel organism mainly grew between 4 degrees c and 37 degrees c, was neutrophilic and slightly halophilic, tolerating up to 10 % nacl. strain r10sw13(t) was haemolytic and w ...	2005	15879234
[preparation of oligosaccharides from alginate by fermenting combined with membrane separation method and analysis of the oligomers].	changes in biomass, oligosaccharides content and alginate lyase activity in broth of alteromonas sp. incubated in shaking flask and fermentation jar were studied. according to those changes, parameters were established for preparing oligosaccharides from alginate by fermentation combined with nano-filtration membrane separation method. resulting oligosaccharides were analyzed by gel permeation chromatography and thin layer chromatography. one liter culture medium(ph 7.5) contains 5 g yeast extra ...	2005	15989283
relationship between temporary inhibition and structure of disulfide-linkage analogs of marinostatin, a natural ester-linked protein protease inhibitor.	a 12-residue marinostatin [mst(1-12): (1)fatmrypsdsde(12)] which contains two ester linkages of thr(3)-asp(9) and ser(8)-asp(11) strongly inhibits subtilisin. in order to study the relationship between the inhibitory activity, structure, and stability of mst, mst analogs were prepared by changing ester linkages to a disulfide linkages. the analogs without the disulfide linkage between 3 and 9 positions lost their inhibitory activity. the k(i) value of 1ss(c(3)-c(9)) ((1)facmrypscsde(12)), which ...	2005	16000118
maltose adaptive mutant of heterotrophic marine bacterium, alteromonas espejiana bal-31: changes in the growth and the induction of extracellular alpha-amylase.	growth of the heterotrophic marine bacterium, alteromonas espejiana bal-31 was inhibited in the presence of sucrose, maltose and even glucose, but not with starch. extracellular alpha-amylase was induced with a lag phase of 2 h in the presence of starch. in contrast, cell growth of the s2a mutant was not affected by the addition of maltose, and starch was ineffective in the induction of extracellular alpha-amylase in this mutant. activity of extracellular alpha-amylase was induced from the s2a m ...	2005	16107754
role of the n-terminal polycystic kidney disease domain in chitin degradation by chitinase a from a marine bacterium, alteromonas sp. strain o-7.	the aim of study was to clarify whether the polycystic kidney disease (pkd) domain of chitinase a (chia) participates in the hydrolysis of powdered chitin.	2005	16108796
resistance of marine bacterioneuston to solar radiation.	a total of 90 bacterial strains were isolated from the sea surface microlayer (i.e., bacterioneuston) and underlying waters (i.e., bacterioplankton) from two sites of the northwestern mediterranean sea. the strains were identified by sequence analysis, and growth recovery was investigated after exposure to simulated solar radiation. bacterioneuston and bacterioplankton isolates were subjected to six different exposure times, ranging from 0.5 to 7 h of simulated noontime solar radiation. followin ...	2005	16151115
iron(iii) coordination chemistry of alterobactin a: a siderophore from the marine bacterium alteromonas luteoviolacea.	alterobactin a is a siderophore produced by the oceanic bacterium alteromonas luteoviolacea. the thermodynamic stability constant of the ferric alterobactin a (alt-a) complex was estimated from electrochemical measurements on the basis of a previously reported linear relationship between the reduction potentials and the ph-independent stability constants for known iron(iii) complexes. the reduction potential of the ferric alterobactin a complex determined by square wave voltammetry is -0.972 v v ...	2005	16212394
salinimonas chungwhensis gen. nov., sp. nov., a moderately halophilic bacterium from a solar saltern in korea.	a halophilic non-spore-forming bacterium of the gamma-proteobacteria, designated strain bh030046t, was isolated from a solar saltern in korea. cells were gram-negative, chemoheterotrophic, short rod-shaped and motile with a polar flagellum. comparative 16s rrna gene sequence analysis revealed that strain bh030046t formed a distinct lineage in the family alteromonadaceae and was distinguished from its closest related genera alteromonas (91.4-94.8 %), aestuariibacter (92.1-93.5 %) and glaciecola ( ...	2005	15653881