Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cloning of gene responsible for tributyltin chloride (tbtc1) resistance in tbtc1-resistant marine bacterium, alteromonas sp. m-1. | a genetic library of tributyltin chloride (tbtc1)-resistant marine bacterium, alteromonas sp. m-1, was constructed using plasmid vector puc 19. three positive clones were obtained from e. coli jm 109 transformed with the plasmids by the method of replica plating to lb medium containing 1 mm tbtc1. these clones could grow in lb liquid medium containing 100 microm tbtc1. plasmids harbouring genes of alteromonas sp. m-1 were designated ptbt1, ptbt2 and ptbt3 which contain 1.8 kb hind iii-fragment, ... | 1993 | 8343157 |
| [structure of the repeating unit of acid polysaccharide from alteromonas sp. 4ms17]. | an acidic polysaccharide from alteromonas sp. 4mc17 is built up of trisaccharide repeating units containing d-glucose, d-mannose and d-galacturonic acid residues. on the basis of methylation studies, 1h and 13c nmr-spectroscopy data, including two-dimensional homonuclear correlation spectroscopy and nuclear overhauser effects, the following structure was suggested for the polysaccharide repeating unit: -->4)-beta-d-glcp-(1-->4)-beta-d-galpa-(1-->4)-beta-d-manp-( 1-->. | 1993 | 8373453 |
| [structure of the repeating unit of acid polysaccharide from alteromonas macleodii 2mm6]. | an acidic polysaccharide from alteromonas macleodii 2mm6 is shown to consist of tetrasaccharide repeating units containing d-galactose, 3-o-acetyl-2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-l-guluronic acid and 3,6-dideoxy-3-(4-hydroxybutyramido)-d-galactose residues. on the basis of sugar analysis, 1h and 13c nmr-spectroscopy data including noe and one-dimensional hohaha experiments, the following structure was suggested for the polysaccharide repeating unit: [formula: see text] r: -co- ... | 1993 | 8373454 |
| effect of phosphate concentration on production of tetrodotoxin by alteromonas tetraodonis. | tetrodotoxin production by alteromonas tetraodonis occurred during the stationary phase of growth and was regulated by phosphate concentration; toxin production was repressed if phosphate was added at the onset of stationary phase and was over 100-fold greater in phosphate-limited cultures than in cultures in which phosphate was not limiting. | 1993 | 8285702 |
| cloning and sequence of an alkaline serine protease-encoding gene from the marine bacterium alteromonas sp. strain o-7. | the gene (aprii) encoding alkaline serine protease (aprii; subtilase) from alteromonas sp. strain o-7 was cloned in plasmid puc19 and transformed into escherichia coli jm109. the nucleotide (nt) sequence of aprii has been determined. a single open reading frame (orf) encoded a protein consisting of 621 amino acids (aa) with a m(r) of 63,958. the results of aa sequence analysis indicated that aprii is produced as a large precursor consisting of four domains: the signal sequence, the n-terminal pr ... | 1993 | 8294010 |
| phylogenetic relationships of marine bacteria, mainly members of the family vibrionaceae, determined on the basis of 16s rrna sequences. | the phylogenetic relationships of 50 reference strains, mostly marine bacteria which require na+ for growth, were determined on the basis of 600 16s rrna nucleotides by using reverse transcriptase sequencing. strains belonging to 10 genera were included (four genera of the family vibrionaceae, the genus aeromonas of the family aeromonadaceae, and the genera alteromonas, marinomonas, shewanella, pseudomonas, and deleya). the sequences were aligned, the similarity values and evolutionary distance ... | 1993 | 8427811 |
| [structure of the repeating link of the acid polysaccharide of alteromonas haloplanktis kmm 156]. | an acidic capsular and an o-specific polysaccharide were isolated from the marine microorganism alteromonas haloplanktis kmm 156. both polysaccharides have the identical structure and are built up of tetrasaccharide repeating units, containing two residues of l-rhamnose as well as a 2-acetamido-2-deoxy-d-glucose and a 3-o-[(r)-1-carboxyethyl]-d-glucose (glc3lac) residue. on the basis of methylation studies, 1h- and 13c-nmr-spectroscopy including nuclear overhauser effect and two-dimensional hete ... | 1993 | 8489533 |
| a siderophore from a marine bacterium with an exceptional ferric ion affinity constant. | virtually all microorganisms require iron for growth. the paucity of iron in surface ocean water (approximately 0.02-1.0 nm (refs 1, 2)) has spurred a lively debate concerning iron limitation of primary productivity, yet little is known about the molecular mechanisms used by marine microorganisms to sequester iron. terrestrial bacteria use a siderophore-mediated ferric uptake system. a siderophore is a low-molecular-mass compound with a high affinity for ferric ion which is secreted by microorga ... | 1993 | 8247152 |
| thiomarinol, a new hybrid antimicrobial antibiotic produced by a marine bacterium. fermentation, isolation, structure, and antimicrobial activity. | thiomarinol, an antimicrobial antibiotic, was isolated from the culture broth of a marine bacterium, alteromonas rava sp. nov. sank 73390. its structure was deduced as a hybrid composed of a pseudomonic acid analogue and holothin by nmr spectral analysis and chemical degradation. antimicrobial activity against gram-positive and gram-negative bacteria of thiomarinol was stronger than both of pseudomonic acids and pyrrothine antibiotics. | 1993 | 8294241 |
| screening of halophilic bacteria and alteromonas species for organophosphorus hydrolyzing enzyme activity. | previously, a g-type nerve agent degrading enzyme activity was found in a halophilic bacterial isolate designated jd6.5. this organism was tentatively identified as an unknown species of the genus alteromonas. in order to determine whether this type of enzyme activity was common in other species of alteromonas, a screening program was initiated. a number of alteromonas species and five halophilic bacterial isolates were cultured and their crude cell extracts screened for hydrolytic activity agai ... | 1993 | 8393735 |
| site-directed mutagenesis of chitinase from alteromonas sp. strain o-7. | chitinase (chi85) from alteromonas sp. strain o-7 contains the two conserved regions common to microbial and plant chitinases. we did site-directed mutagenesis of chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. we suggest that asp-290 and glu-292 of chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin. | 1993 | 7764022 |
| cloning, sequence, and expression of a chitinase gene from a marine bacterium, altermonas sp. strain o-7. | the gene encoding an extracellular chitinase from marine alteromonas sp. strain o-7 was cloned in escherichia coli jm109 by using puc18. the chitinase produced was not secreted into the growth medium but accumulated in the periplasmic space. a chitinase-positive clone of e. coli produced two chitinases with different molecular weights from a single chitinase gene. these proteins showed almost the same enzymatic properties as the native chitinase of alteromonas sp. strain o-7. the n-terminal sequ ... | 1993 | 8416892 |
| purification and properties of a highly active organophosphorus acid anhydrolase from alteromonas undina. | a highly active organophosphorus acid anhydrolase from alteromonas undina was purified to homogeneity and found to be composed of a single polypeptide chain with a molecular weight of 53,000. with diisopropylfluorophosphate as a substrate, the purified enzyme has a specific activity of approximately 575 mumol/min/mg of protein. the enzyme has optimum activity at ph 8.0 and 55 degrees c and is stimulated by sulfhydryl reducing agents and manganese. it is capable of rapidly hydrolyzing a wide rang ... | 1993 | 16349054 |
| flow cytometric analysis of the cellular dna content of salmonella typhimurium and alteromonas haloplanktis during starvation and recovery in seawater. | flow cytometry was used to investigate the heterogeneity of the dna content of salmonella typhimurium and alteromonas haloplanktis cells that were starved and allowed to recover in seawater. hoechst 33342 (bisbenzimide) was used as a dna-specific dye to discriminate between dna subpopulations. the dna contents of both strains were heterogeneous during starvation. s. typhimurium cells contained one or two genomes, and a. haloplanktis cells contained up to six genomes. s. typhimurium genomes were ... | 1994 | 7811075 |
| [distribution of alpha-n-acetylgalactosaminidases in marine and freshwater microorganisms]. | the distribution of the alpha-n-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the pacific ocean and 423 baikalian microorganisms from fresh water and sponges have been studied. about 24% as marine as baikalian strains produced this enzyme. the most number of alpha-n-ac-galactosidase producers were found for marine bacteria genera aeromonas, alteromonas and vibrio (71% a ... | 1994 | 7898396 |
| characterization of an antibiotic produced by alteromonas luteoviolacea gauthier 1982, 85 isolated from kinko bay, japan. | an antibiotic produced by alteromonas luteoviolacea strain 9k-v10 was recovered after cold acetone precipitation of culture supernatant fluids or lysates that had been frozen and thawed. the precipitate obtained from cell-free lysates was fractioned by deae ion-exchange chromatography. further purification by gel-filtration chromatography yielded a single peak of antibiotic activity that corresponded to a protein peak with a molecular mass of approximately 100 kda. after non-denaturing polyacryl ... | 1994 | 7989271 |
| isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities. | lytic digestion of poly(mannuronate), poly(guluronate), and alginate with an alginate lyase from alteromonas macleodii was used to prepare mixtures of unsaturated oligosaccharides. four oligosaccharides isolated from the alginate lyase-lysate by anion-exchange chromatography on q-sepharose were found to be the major components of the root growth-promoting lysate. the oligosaccharides were analyzed by nmr and sims and identified as di- and tri-saccharides having o-(4-deoxy-l-erythro-hex-4-enopyra ... | 1994 | 8039175 |
| gene sequence, purification and characterization of n-acetyl-beta-glucosaminidase from a marine bacterium, alteromonas sp. strain o-7. | the gene (cht60) encoding n-acetyl-beta-glucosaminidase (cht; ec 3.2.1.30) from the marine bacterium alteromonas sp. strain o-7 was cloned into puc18 in escherichia coli jm109. the nucleotide (nt) sequence of cht60 was determined. a 1797-bp open reading frame encoded a polypeptide of 598 amino acids (aa) (m(r) 64,535). the aa sequence of the cloned enzyme (cht60) deduced from the nt sequence showed no significant sequence homologies with available aa sequences from databases. cht60 was purified ... | 1994 | 8063094 |
| the gene encoding the kappa-carrageenase of alteromonas carrageenovora is related to beta-1,3-1,4-glucanases. | the nucleotide (nt) and deduced amino acid (aa) sequences are reported for the structural gene cgka encoding kappa-carrageenase (m(r) 44,412) from the marine bacterium alteromonas carrageenovora (atcc 43555), a hydrolase involved in the degradation of kappa-carrageenan. the cgka gene codes for a protein of 397 aa, with a signal peptide of 25 aa. the enzyme is a new member of family 16 of glycosyl hydrolases, which comprises beta-1,3-1,4-glucanases from various sources and the beta-agarase of str ... | 1994 | 8112578 |
| dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31p nmr. | the dynamic properties of nucleic acids in five different types of intact supramolecular systems, namely, chicken erythrocyte chromatin, the wild type and a deletion mutant of the lambda phage, lipid-containing phage pm2, and alteromonas espejiana ribosomes, were investigated by means of 31p solid-state nmr. the nucleic acids in the different supramolecular systems showed unique dynamic properties, which are closely connected with their functions. the total anisotropy of the phosphorus chemical ... | 1994 | 8206876 |
| phylogenetic analysis and assessment of the genera vibrio, photobacterium, aeromonas, and plesiomonas deduced from small-subunit rrna sequences. | we sequenced nearly complete small-subunit rrnas of 54 reference strains belonging to the genera vibrio, photobacterium, aeromonas, and plesiomonas. we then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony ... | 1994 | 7520733 |
| the 16s rrna sequence and genome sizing of tributyltin resistant marine bacterium, strain m-1. | the 16s rrna of the tributyltin resistant marine bacterium, strain m-1, was partly sequenced to confirm the taxonomic status. the results indicated that this bacterium should be classified under the genus alteromonas, instead of a previous report in which this strain was identified as a vibrio. the genome size of this strain was also measured by pulsed field gel electrophoresis (pfge) using a contoureclamped homogeneous electric field. the strain was found to contain a genome size of 2,240 kilo ... | 1994 | 7512189 |
| sequence similarities and evolutionary relationships of microbial, plant and animal alpha-amylases. | amino acid sequence comparison of 37 alpha-amylases from microbial, plant and animal sources was performed to identify their mutual sequence similarities in addition to the five already described conserved regions. these sequence regions were examined from structure/function and evolutionary perspectives. an unrooted evolutionary tree of alpha-amylases was constructed on a subset of 55 residues from the alignment of sequence similarities along with conserved regions. the most important new infor ... | 1994 | 7925367 |
| stability and structural analysis of alpha-amylase from the antarctic psychrophile alteromonas haloplanctis a23. | the alpha-amylase secreted by the antarctic bacterium alteromonas haloplanctis displays 66% amino acid sequence similarity with porcine pancreatic alpha-amylase. the psychrophilic alpha-amylase is however characterized by a sevenfold higher kcat and kcat/km values at 4 degrees c and a lower conformational stability estimated as 10 kj.mol-1 with respect to the porcine enzyme. it is proposed that both properties arise from an increase in molecular flexibility required to compensate for the reducti ... | 1994 | 8020481 |
| [studies on fugu (globefish) tetrodotoxin over one century]. | one century has passed since fugu toxin was first named tetrodotoxin by tahara. chemical problems such as crystallization of tetrodotoxin, followed by structure determination by the research groups headed by tsuda, hirata, woodward, and mosher, and its subsequent synthesis by kishi have been solved. in the last 10 years, research on the distribution of tetrodotoxin in nature has shown that tetrodotoxin is not produced by fugu fish, but rather is formed by sea bacteria such as vibrio, alteromonas ... | 1994 | 11613510 |
| production and characterization of an exopolysaccharide excreted by a deep-sea hydrothermal vent bacterium isolated from the polychaete annelid alvinella pompejana. | the heterotrophic and mesophilic marine bacterium hyd-1545 was isolated on a metal-amended medium from the dorsal integument of the hydrothermal vent polychaete alvinella pompejana. this strain, which can be assigned to the genus alteromonas on the basis of its g+c content and phenotypical features, produced large amounts of an acidic polysaccharide in batch cultures. the polysaccharide was excreted during the stationary phase of growth and contained glucose, galactose, glucuronic acid, galactur ... | 1994 | 16349441 |
| purification and characterization of beta-n-acetylglucosaminidase from alteromonas sp. strain o-7. | beta-n-acetylglucosaminidase (ec 3.2.1.30) was purified from the outer membrane of a marine bacterium, alteromonas sp. strain o-7. the enzyme (glcnacase a) was purified by successive column chromatographies. the purified enzyme was found to be homogeneous on sodium dodecyl sulfate polyacrylamide gel electrophoresis. the molecular mass and pi of glcnacase a were 92kda and 4.9, respectively. the optimum ph and temperature were 6.0-7.0 and 45 degrees c, respectively. glcnacase a was stable up to 40 ... | 1995 | 7613001 |
| phylogenetic diversity of the bacterial community from a microbial mat at an active, hydrothermal vent system, loihi seamount, hawaii. | the phylogenetic diversity of small-subunit rrna genes associated with the domain bacteria was examined (by using previously defined operational taxonomic units [c. l. moyer, f.c. dobbs, and d. m. karl, appl. environ. microbiol. 60:871-879, 1994]; those for pele's vents bacteria are hereafter abbreviated pvb otus) with samples from a microbial mat at an active, deep-sea hydrothermal vent system. a cluster of phylogenetically related pvb otus (otus 2, 3, 6, and 8) was closely affiliated with thio ... | 1995 | 7538279 |
| phylogenetic analysis of the genera alteromonas, shewanella, and moritella using genes coding for small-subunit rrna sequences and division of the genus alteromonas into two genera, alteromonas (emended) and pseudoalteromonas gen. nov., and proposal of twelve new species combinations. | small-subunit ribosomal dna sequences were determined for 17 strains belonging to the genera alteromonas, shewanella, vibrio, and pseudomonas, and these sequences were analyzed by phylogenetic methods. the resulting data confirmed the existence of the genera shewanella and moritella, but suggested that the genus alteromonas should be split into two genera. we propose that a new genus, the genus pseudoalteromonas, should be created to accommodate 11 species that were previously alteromonas specie ... | 1995 | 7547295 |
| comparison of the fatty acids of the tunicate botryllus schlosseri from the black sea with two associated bacterial strains. | the fatty acid composition of the tunicate botryllus schlosseri and of two bacterial strains found within the tunicate, namely vibrio parahaemolyticus and of an associated but previously unreported gram positive cocci were studied. the polyunsaturated fatty acids 6,9,12-octadecatrienoic acid, 5,8,11,14,17-eicosapentaenoic acid, and 4,7,10,13,16,19-docosahexaenoic acid were particularly abundant in b. schlosseri and were not detected in the two bacterial strains found in the tunicate. the iso/ant ... | 1995 | 7564924 |
| processing and hydrolytic mechanism of the cgka-encoded kappa-carrageenase of alteromonas carrageenovora. | the cgka gene of alteromonas carrageenovora encodes a kappa-carrageenase with a predicted mass of 44212 da, much larger than the 35 kda estimated from sds/page of the protein purified from culture supernatants. immunoblotting experiments showed the presence of a protein of 44 +/- 2 kda in both native and recombinant bacterial intracellular extracts, suggesting that the kappa-carrageenase is produced as a preproprotein which undergoes proteolytic processing twice during secretion. to determine th ... | 1995 | 7737202 |
| in situ biodegradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in natural waters. | the biodegradation of samples of poly(3-hydroxybutyrate)(p(3hb)), poly(3-hydroxybutyrate-co-10%-3-hydroxyvalerate)(p(3hb-co-10 %-3hv)), and poly 3-hydroxybutyrate-co-20%-3-hydroxyvalerate)(p(3hb-co-20%- 3hv)) was investigated in situ in natural waters. the degradation was studied by decrease in mass, molecular weight, and tensile strength. in two freshwater ponds the polymers were slowly degraded. after half a year of submersion the mass loss was less than 7%. after 358 days in a freshwater cana ... | 1995 | 7606659 |
| tributyltin-resistant marine bacteria: a summary of recent work. | a tributyltin chloride (tbtcl)-resistant bacterium, alteromonas sp. m-1, was isolated from coastal seawater. this bacterium grew in medium containing 125 microm tbtcl. tbtcl added to the medium was taken up by this bacterium, however, the amount of tbtcl in the cellular fraction was low after the logarithmic phase, suggesting the existence of a tbtcl-efflux system. a genetic library was constructed using plasmid vector puc 19. three positive clones were obtained, by which e. coli was transformed ... | 1995 | 7766207 |
| kinetics and mechanism of bal 31 nuclease action on small substrates and single-stranded dna. | kinetic and mechanistic aspects of the action of two forms of the bal 31 nuclease (ec 3.1.11) from alteromonas espejiana on model substrates, small oligonucleotides, larger oligonucleotides and poly[d(a)] have been examined. the minimal oligonucleotide substrate is a 5'-phosphorylated dinucleotide and a phosphodiester not containing a nucleotide residue is not cleaved. both forms act predominantly in an exonucleolytic fashion on single-stranded dna polymers in a highly processive manner; however ... | 1995 | 7669801 |
| in vitro activity of bms-181139, a new carbapenem with potent antipseudomonal activity. | the in vitro activities of the carbapenem bms-181139 were determined in comparison with those of imipenem, meropenem, ciprofloxacin, ceftriaxone, and vancomycin. bms-181139 was the most active against species of pseudomonas and related genera alteromonas and burkholderia, with mics for 147 of 149 isolates of < 4 micrograms/ml. of 22 imipenem-resistant (mic > 8 micrograms/ml) p. aeruginosa strains, only 1 required an mic of bms-181139 of > 4 micrograms/ml, compared with 14 requiring the same mero ... | 1995 | 7726502 |
| identification of the positions of disulfide bonds of chitinase from a marine bacterium, alteromonas sp. strain o-7. | extracellular chitinase from marine alteromonas sp. strain o-7 is unique because of the activation by four major cations contained in sea water, such as na+, k+, mg2+, and ca2+. the positions of s-s bonds of alteromonas chitinase were identified. alteromonas chitinase was fragmented by tpck-trypsin and staphylococcus aureus v8 protease. the amino acid and sequence analyses of three peptides showed that the positions of disulfide bonds are cys(94)-cys(99), cys(174)-cys(196), and cys(386)-cys(395) ... | 1995 | 8534997 |
| molecular cloning of the gene which encodes beta-n-acetylglucosaminidase from a marine bacterium, alteromonas sp. strain o-7. | the gene encoding the periplasmic beta-n-acetylglucosaminidase (glcnacase b) from a marine alteromonas sp. strain, o-7, was cloned and sequenced. the protein sequence of glcnacase b revealed a highly significant homology with vibrio glcnacase and alpha- and beta-chains of human beta-hexosaminidase. | 1995 | 7574618 |
| a comparative study of pcr product detection and quantitation by electro-chemiluminescence and fluorescence. | amplification and detection of target dna sequences are made possible in a polymerase chain reaction (pcr) by using a mixture of biotinylated and ruthenium(ii) trisbipyridal (ru(bpy)3(2+))-end-labelled primers. in this way, biotin for capture and ru(bpy)3(2+) for detection are directly incorporated into the pcr product obviating subsequent probe hybridization. pcr of a bacterial dna template from alteromonas species strain jd6.5 using a cocktail of biotin- and ru(bpy)3(2+)-labelled primers ampli ... | 1995 | 8533605 |
| arylsulphatase from alteromonas carrageenovora. | arylsulphatase activity was identified in cultures of the marine bacterium alteromonas carrageenovora, using methylumbelliferyl sulphate as substrate. in contrast with most other microbial arylsulphatases, arylsulphatase production in a. carrageenovora was not repressed by sulphate. the structural gene of arylsulphatase (atsa) was cloned and sequenced. an orf of 984 bp was found, specifying a primary translation product of 328 amino acids with a molecular mass of 35797 da. arylsulphatase was par ... | 1995 | 8535517 |
| structure of the capsular polysaccharide from alteromonas sp. cmm 155. | capsular polysaccharide (cps) was obtained by water-saline extraction of the alteromonas sp. cmm 155. on the basis of solvolysis with anhydrous hf and 1h- and 13c-nmr spectral data, including noe experiments, it was concluded that the capsular polysaccharide had the following structure containing novel n-acyl-amino sugar and bacillosamine residues: --> 3)-alpha-d-galpnac-(1 --> 4)-alpha-l-galapnac(1 --> 3)- alpha-d-quipnac4nac-(1 --> 3)-beta-d-quip4nalaac-(1 --> | 1995 | 7585719 |
| structural and functional aspects of chloride binding to alteromonas haloplanctis alpha-amylase. | chloride is the allosteric effector of vertebrate pancreatic and salivary alpha-amylases and of the bacterial alpha-amylase from alteromonas haloplanctis. activation experiments of a. haloplanctis alpha-amylase by several monovalent anions show that a negative charge, not restricted to that of cl-, is essential for the amylolytic reaction. engineering of the chloride binding site reveals that a basic residue is an essential component of the site. the mutation k337r alters the cl--binding propert ... | 1996 | 8798613 |
| description of a new polymer-secreting bacterium from a deep-sea hydrothermal vent, alteromonas macleodii subsp. fijiensis, and preliminary characterization of the polymer. | a deep-sea, aerobic, mesophilic, heterotrophic bacterium was isolated from fluid collected near an active hydrothermal vent. on the basis of phenotypic and phylogenetic analyses and dna-dna relatedness, strain st716 could be assigned to the species alteromonas macleodii as a new subspecies. this bacterium secreted an unusual high-molecular-weight polysaccharide in the presence of glucose in batch cultures. the viscosity of this exopolysaccharide is of the same order of magnitude as that of xanth ... | 1996 | 8572714 |
| cloning and expression of a gene encoding a bacterial enzyme for decontamination of organophosphorus nerve agents and nucleotide sequence of the enzyme. | organophosphorus acid (opa) anhydrolase enzymes have been found in a wide variety of prokaryotic and eukaryotic organisms. interest in these enzymes has been prompted by their ability to catalyze the hydrolysis of toxic organophosphorus cholinesterase-inhibiting compounds, including pesticides and chemical nerve agents. the natural substrates for these enzymes are unknown. the gene (opaa) which encodes an opa anhydrolase (opaa-2) was isolated from an alteromonas sp. strain jd6.5 ecori-lambda zap ... | 1996 | 8633861 |
| characterization and solution properties of a new exopolysaccharide excreted by the bacterium alteromonas sp. strain 1644. | this paper concerns the study of a new bacterial exopolysaccharide. the ionic properties of the polymer are considered in relation to the first structural results. this polysaccharide is a carboxylic polyelectrolyte with a charge parameter of 1.33, and exhibits usual polyelectrolyte properties with monovalent and mg2+ ions. in contrast, its affinity for the divalent ions ca2+, sr2+ and ba2+ is greater than predicted from electrostatic theory. in solution, the polysaccharide undergoes a local con ... | 1996 | 8852748 |
| cloning and sequence analysis of a protease-encoding gene from the marine bacterium alteromonas sp. strain o-7. | the gene (apri) encoding alkaline serine protease (apri; subtilase) from alteromonas sp. strain o-7 was cloned and sequenced. the nucleotide sequence of apri has been identified. the deduced amino acid sequence indicated that apri codes for a precursor of 715 amino acids and the precursor is composed of four regions including a signal peptide, an n-terminal pro-region, a mature protease region and a c-terminal extension region of 215 amino acids as previously described for aprii [h. tsujibo et a ... | 1996 | 8987544 |
| solution and gel rheology of a new polysaccharide excreted by the bacterium alteromonas sp. strain 1644. | a new bacterial polysaccharide '1644' is studied in aqueous solutions and in the gel state. the viscometric measurements for dilute and semi-dilute solutions in presence of monovalent salts are usual; however, in presence of divalent cations like ca2+ an original behaviour is observed, related to intermolecular bonds. for larger polymer concentrations a clear gel is obtained with divalent counterions. this gel exhibits unusual properties compared to other gelling polysaccharides: it is very elas ... | 1996 | 8852757 |
| crystallization and preliminary x-ray diffraction studies of alpha-amylase from the antarctic psychrophile alteromonas haloplanctis a23. | a cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile alteromonas haloplanctis a23 grown at 4 degrees c. in order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold-adapted enzyme have been initiated because a three-dimensional structure of a mesophilic counterpart, pig pancreatic alpha-amylase, already exists. alpha-amylase from a. haloplanctis, which shares 53% sequence identity with pi ... | 1996 | 8897615 |
| structure determination of a novel uronic acid residue isolated from the exopolysaccharide produced by a bacterium originating from deep sea hydrothermal vents. | the exopolysaccharide produced by the bacterium alteromonas sp. strain 1644 originating from deep sea hydrothermal vents was shown to contain a novel glucuronic acid derivatives: 3-o-[(r)-1-carboxyethyl]-d-glucuronic acid. the structure of this compound was established on the basis of mass spectrometric data, methylation analysis, preparation of derivatives, and chemical synthesis of references compounds. | 1996 | 8823906 |
| automated systems for identification of heterotrophic marine bacteria on the basis of their fatty acid composition. | the fatty acid methyl ester composition of a total of 71 marine strains representing the genera alteromonas, deleya, oceanospirillum, and vibrio was determined by gas-liquid chromatographic analysis. over 70 different fatty acids were found. the predominant fatty acids were 16:0, 16:1 cis 9, summed-in-feature (sif) 4 (15:0 iso 2oh and/or 16:1 trans 9) and sif 7 (18:1 cis 11, 18:1 trans 9, and/or 18:1 trans 6) for all the strains considered, but minor quantitative variations could be used to dist ... | 1996 | 16535340 |
| molecular cloning and characterization of a novel beta-n-acetyl-d-glucosaminidase from vibrio furnissii. | the accompanying papers (keyhani, n. o., and roseman, s. (1996) j. biol. chem. 271, 33414-33424; keyhani, n. o., and roseman, s. (1996) j. biol. chem. 271, 33425-33432) describe two unique beta-n-acetylglucosaminidases from vibrio furnissii. a third, exoii, is reported here. the gene, exoii, was cloned into escherichia coli, sequenced, and exoii purified to apparent homogeneity (36 kda). the molecular weight and n-terminal 16 amino acids of the protein conform to the predicted sequence. exoii ex ... | 1996 | 8969206 |
| structure of the capsular polysaccharide from alteromonas nigrifaciens iam 13010t containing 2-acetamido-2,6-dideoxy-l-talose and 3-deoxy-d-manno-octulosonic acid. | a capsular polysaccharide was obtained from alteromonas nigrifaciens iam 13010t by saline extraction. on the basis of 1h and 13c nmr spectroscopy, including one-dimensional (1d) noe spectroscopy, 2d rotating-frame noe spectroscopy (roesy), and 1h-detected heteronuclear 1h,13c multiple-quantum coherence (hmqc), it was concluded that the polysaccharide contained inter alia an acidic sugar, 3-deoxy-d-manno-octulosonic acid (kdo), and a rare amino sugar, 2-acetamido-2,6-dideoxy-l-talose (l-6dtalnac, ... | 1997 | 9129296 |
| alteromonas infernus sp. nov., a new polysaccharide-producing bacterium isolated from a deep-sea hydrothermal vent. | a deep-sea, aerobic, mesophilic and heterotrophic new bacterium was isolated from a sample of fluid collected among a dense population of riftia pachyptila, in the vicinity of an active hydrothermal vent of the southern depression of the guaymas basin (gulf of california). on the basis of phenotypic and phylogenetic analyses and dna/dna relatedness, the strain gy785 was recognized as a new species of the genus alteromonas and the name of alteromonas infernus is proposed. during the stationary ph ... | 1997 | 9134716 |
| a novel metalloproteinase, almelysin, from a marine bacterium, alteromonas sp. no. 3696: purification and characterization. | we have discovered a novel metalloproteinase, which has high activity at low temperatures, from the culture supernatant of a marine bacterium. the strain was identified as alteromonas sp. no. 3696. the metalloproteinase, named almelysin, was purified to homogeneity from the cultured supernatant at 10 degrees c by two column chromatographies. about 20 mg of purified almelysin was obtained from 18.4 liters of the culture supernatant. the molecular mass of almelysin was estimated to be 28 kda by sd ... | 1997 | 9145531 |
| shewanella woodyi sp. nov., an exclusively respiratory luminous bacterium isolated from the alboran sea. | thirty-four strains of nonfermentative, respiratory, luminous bacteria were isolated from samples of squid ink and seawater from depths of 200 to 300 m in the alboran sea. although these strains had a few properties similar to properties of shewanella (alteromonas) hanedai, they did not cluster phenotypically with any previously described bacterium. the nucleotide sequence of a 740-bp segment of luxa was not homologous with other known luxa sequences but clustered with the luxa sequences of shew ... | 1997 | 9336902 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| nucleotide sequence of a gene encoding an organophosphorus nerve agent degrading enzyme from alteromonas haloplanktis. | organophosphorus acid anhydrolases (opaa) catalyzing the hydrolysis of a variety of toxic organophosphorus cholinesterase inhibitors offer potential for decontamination of g-type nerve agents and pesticides. the gene (opa) encoding an opaa was cloned from the chromosomal dna of alteromonas haloplanktis atcc 23821. the nucleotide sequence of the 1.7 -kb dna fragment contained the opa gene (1.3 kb) and its flanking region. we report structural and functional similarity of opaas from a. haloplankti ... | 1997 | 9079288 |
| a pluripotent polyphenol oxidase from the melanogenic marine alteromonas sp shares catalytic capabilities of tyrosinases and laccases. | the recently characterized marine melanogenic bacterium mmb-1 contains a pluripotent polyphenol oxidase (ppo) which catalyzes the oxidation of a very wide range of substrates considered specific for tyrosinase or laccase. this range includes monophenols such as l-tyrosine, o-diphenols such as l-dopa, p-diphenols such as hydroquinone, o-aminophenols such as 3-hydroxyanthranilic acid, activated monophenols such as 2,6-dimethoxyphenol and syringaldazine, and chromophores such as abts. this is the f ... | 1997 | 9398646 |
| novel alginate lyases from marine bacterium alteromonas sp. strain h-4. | a bacterium alteromonas sp. strain h-4 isolated from laminaria fronds produced extra- and intra-cellular alginate lyases and utilized alginate as its sole carbon source. an extracellular alginate lyase was purified from the culture supernatant of the strain and its substrate specificity was characterized. the estimated molecular mass of the enzyme was 32 kda and the isoelectric point was 4.7. both polym and polyg block degrading activities were observed using the substrate-containing gel overlay ... | 1997 | 9403996 |
| diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
| isolation and characterization of strain mmb-1 (cect 4803), a novel melanogenic marine bacterium. | a novel marine melanogenic bacterium, strain mmb-1, was isolated from the mediterranean sea. the taxonomic characterization of this strain indicated that it belongs to the genus alteromonas. under in vivo conditions, l-tyrosine was the specific monophenolic precursor for melanin synthesis. this bacterium contained all types of activities associated with polyphenol oxidases (ppos), cresolase (ec 1.18.14.1), catecholase (ec 1.10.3.1), and laccase (ec 1.10.3.2). these activities were due to the pre ... | 1997 | 16535688 |
| g-type nerve agent decontamination by alteromonas prolidase. | 1998 | 9928098 | |
| pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of laminaria japonica. | an aerobic, polarly flagellated marine bacterium that produces a prodigiosin-like pigment was isolated from the red-spotted culture beds of laminaria japonica. five isolates had unique bacteriolytic activity for both gram-positive and -negative bacteria, which had never been observed among alteromonas or related species. the isolates were identified as the causative agent of red spot disease of l. japonica seeds. the phenotypic features of the isolates were similar to these of pseudoalteromonas ... | 1998 | 9734030 |
| specific inhibition of chemiluminescent activity by pathogenic vibrios in hemocytes of two marine bivalves: pecten maximus and crassostrea gigas. | hemocytes from two adult bivalves, pecten maximus and crassostrea gigas, were exposed to 12 different bacterial strains including 2 alteromonas spp. (u1 and t413), 2 type strains of vibrios (v. anguillarum atcc 19264 and v. alginolyticus atcc 17749), 1 vibrio (s322) pathogenic to c. gigas larvae, 2 vibrios (v110 and v117) virulent to ostrea edulis larvae, and 5 different strains of a same vibrio sp. (group a496) isolated from moribund p. maximus larvae. after 1.5 h contact with bacteria, zymosan ... | 1998 | 9446738 |
| structural studies of an exopolysaccharide produced by alteromonas macleodii subsp. fijiensis originating from a deep-sea hydrothermal vent. | the structure of the exopolysaccharide produced by alteromonas macleodii subsp. fijiensis recovered from a deep-sea hydrothermal vent has been investigated. by means of chemical analysis and nmr studies, the repeating unit of the polymer was deduced to be a branched hexasaccharide with the structure shown. [formula: see text] | 1998 | 9836450 |
| characterization of chitinase c from a marine bacterium, alteromonas sp. strain o-7, and its corresponding gene and domain structure. | one of the chitinase genes of alteromonas sp. strain o-7, the chitinase c-encoding gene (chic), was cloned, and the nucleotide sequence was determined. an open reading frame coded for a protein of 430 amino acids with a predicted molecular mass of 46,680 da. alignment of the deduced amino acid sequence demonstrated that chic contained three functional domains, the n-terminal domain, a fibronectin type iii-like domain, and a catalytic domain. the n-terminal domain (59 amino acids) was similar to ... | 1998 | 9464381 |
| crystal structures of the psychrophilic alpha-amylase from alteromonas haloplanctis in its native form and complexed with an inhibitor. | alteromonas haloplanctis is a bacterium that flourishes in antarctic sea-water and it is considered as an extreme psychrophile. we have determined the crystal structures of the alpha-amylase (aha) secreted by this bacterium, in its native state to 2.0 angstroms resolution as well as in complex with tris to 1.85 angstroms resolution. the structure of aha, which is the first experimentally determined three-dimensional structure of a psychrophilic enzyme, resembles those of other known alpha-amylas ... | 1998 | 9541387 |
| phenotypic diversity of pseudoalteromonas citrea from different marine habitats and emendation of the description. | four strains of marine, aerobic, agar-decomposing bacteria with one polar flagellum and with dna g + c contents of 38.9-40.2 mol% were isolated from the far-eastern mussels crenomytilus grayanus and patinopecten yessoensis. these four strains were identified as pseudoalteromonas; however, they were phenotypically different from species described previously according to carbon compound utilization tests and the biolog identification system. high agar-decomposing activity was found in two strains, ... | 1998 | 9542094 |
| characterization of the c-terminal propeptide involved in bacterial wall spanning of alpha-amylase from the psychrophile alteromonas haloplanctis. | the antarctic psychrophile alteromonas haloplanctis secretes a ca2+- and cl--dependent alpha-amylase. the nucleotide sequence of the amy gene and the amino acid sequences of the gene products indicate that the alpha-amylase precursor is a preproenzyme composed by the signal peptide (24 residues), the mature alpha-amylase (453 residues, 49 kda), and a long c-terminal propeptide or secretion helper (192 residues, 21 kda). in cultures of the wild-type strain, the 70-kda precursor is secreted at the ... | 1998 | 9575155 |
| development of a genetic transformation system for an alga-lysing bacterium. | four marine bacteria, alteromonas sp. strains a27, a28, a29, and a30, that lyse the diatom skeletonema costatum nies-324 were isolated from coastal seawater samples. they were also able to lyse the diatoms thalassiosira sp. and eucampia zodiacs and the raphidophycean flagellate chattonella antiqua. cryptic indigenous plasmids, designated pas28 and pas29, were detected in alteromonas sp. strains a28 and a29, respectively. these plasmids appeared to be similar based on size and restriction site an ... | 1998 | 9603814 |
| sialic acid-binding lectin with antibacterial activity from the horse mussel: further characterization and immunolocalization. | a heterogeneous sialic acid-binding lectin with affinity for bacterial lps was isolated and partially characterized from hemolymph of the horse mussel modiolus modiolus.(1) using two-dimensional electrophoresis with immobilized ph gradients, the lectin revealed three subunits with different molecular weight and isoelectric points (pi); mr14 (pi approximately 5.1 and approximately 5.5), 17.5 (pi approximately 5.5) and 20 (pi approximately 4.9) kda. the affinity purified lectin existed in its nati ... | 1998 | 9639084 |
| fatty acid composition of bacteria associated with the toxic dinoflagellate ostreopsis lenticularis and with caribbean palythoa species. | the fatty acid composition of a pseudomonas sp. (alteromonas) and its host, the dinoflagellate ostreopsis lenticularis, vectors in ciguatera fish poisoning, has been studied. the major fatty acids in o. lenticularis were 16:0, 20:5n-3, and 22:6n-3, but 18:2n-6, 18:3n-3, and 18:n-3 were also identified. in contrast to other dinoflagellates, 1 8:5n-3 was not detected in o. lenticularis. even-chain fatty acids such as 9-16:1, 11-18:1, and 13-20:1 predominated in the pseudomonas sp. from o. lenticul ... | 1998 | 9655379 |
| structures of the psychrophilic alteromonas haloplanctis alpha-amylase give insights into cold adaptation at a molecular level. | . enzymes from psychrophilic (cold-adapted) microorganisms operate at temperatures close to 0 degreesc, where the activity of their mesophilic and thermophilic counterparts is drastically reduced. it has generally been assumed that thermophily is associated with rigid proteins, whereas psychrophilic enzymes have a tendency to be more flexible. | 1998 | 9862804 |
| numerical taxonomy of heavy metal-tolerant nonhalophilic bacteria isolated from hypersaline environments. | a total of 232 metal-tolerant bacterial strains were isolated from water and sediment samples collected in different hypersaline environments located in cádiz, huelva and morón de la frontera (spain). they were isolated on a medium containing mercury, chromium, cadmium, copper or zinc. these halotolerant isolates were analyzed by numerical taxonomy techniques by using the simple matching (ssm) and jaccard (sj) coefficients; clustering was achieved using the unweighted pair group method with aver ... | 1998 | 10943340 |
| cloning and nucleotide sequence of the gene encoding a serine proteinase inhibitor named marinostatin from a marine bacterium, alteromonas sp. strain b-10-31. | the gene (msti) encoding a serine proteinase inhibitor named marinostatin from marine alteromonas sp. strain b-10-31 was cloned and its nucleotide sequence was analyzed. a short open reading frame of 192 bp encoded 63 amino acids with a molecular weight of 6,985. furthermore, the initial product of marinostatin (marinostatin l) was purified and its amino acid sequence was analyzed. these results indicate that marinostatin is produced as a unique precursor consisting of the mature peptide and the ... | 1998 | 9972273 |
| purification and characterization of the heat-labile alpha-amylase secreted by the psychrophilic bacterium tac 240b. | a total of 59 bacteria samples from antarctic sea water were collected and screened for their ability to produce alpha-amylase. the highest activity was recorded from an isolate identified as an alteromonas species. the purified alpha-amylase shows a molecular mass of about 50,000 da and a pi of 5.2. the enzyme is stable from ph 7.5 to 9 and has a maximal activity at ph 7.5. compared with other alpha-amylases from mesophiles and thermophiles, the "cold enzyme" displays a higher activity at low t ... | 1999 | 10453473 |
| molecular analysis of the gene encoding a novel transglycosylative enzyme from alteromonas sp. strain o-7 and its physiological role in the chitinolytic system. | we purified from the culture supernatant of alteromonas sp. strain o-7 and characterized a transglycosylating enzyme which synthesized beta-(1-->6)-(glcnac)2, 2-acetamido-6-o-(2-acetamido-2-deoxy-beta-d-glucopyranosyl)-2- deoxyglucopyranose from beta-(1-->4)-(glcnac)2. the gene encoding a novel transglycosylating enzyme was cloned into escherichia coli, and its nucleotide sequence was determined. the molecular mass of the deduced amino acid sequence of the mature protein was determined to be 99, ... | 1999 | 10464221 |
| [spontaneous mutants of alteromonas espejiana, resistant to kanamycin and bleomycin]. | an attempt was made to induce insertions in marine bacterium alteromonas espejiana bal-31 (ae) using the tnphoa transposon. the ae mutants selected on a kanamycin-containing medium after conjugation of the ae with the transposon donor, escherichia coli sm10(prt291), were resistant not only to kanamycin (kn), but also to bleomycin (bm), and were sensitive to tetracycline. although the mutants were phenotypically similar to insertion mutants, the mutations appeared to be spontaneous. the sensitivi ... | 1999 | 10687100 |
| diversity of free-living and attached bacteria in offshore western mediterranean waters as depicted by analysis of genes encoding 16s rrna. | in a previous study (s. g. acinas, f. rodríguez-valera, and c. pedrós-alió, fems microbiol. ecol. 24:27-40, 1997), community fingerprinting by 16s rdna restriction analysis applied to mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 micrometer. here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16s rdna obtai ... | 1999 | 9925576 |
| purification and characterization of a novel beta-agarase from an alkalophilic bacterium, alteromonas sp. e-1. | a novel beta-agarase (ec 3.2.1.81) was purified from an agar-degrading alkalophilic bacterium, alteromonas sp. e-1 isolated from the soil. this enzyme was obtained from a cell-free extract after sonication and purified 40.9-fold through treatment with streptomycin, ammonium sulfate fractionation and successive chromatography on anion-exchange and gel filtration columns. the molecular weight was estimated to be 82 kda by sds-polyacrylamide gel electrophoresis and 180 kda by superdex 200 gel filtr ... | 1999 | 16232496 |
| the effect of motility and cell-surface polymers on bacterial attachment. | recently it was shown that motility of vibrio alginolyticus facilitated cell attachment to glass surfaces. in the present study the same relationship between motility and cell attachment was confirmed for alcaligenes and alteromonas spp. these findings clearly answer a long-standing question: does motility facilitate attachment? however, they are contradictory to a general view on cell attachment that the energy barrier due to electrostatic repulsion between negatively charged bacterial cells an ... | 1999 | 10537201 |
| thermodynamic stability of a cold-active alpha-amylase from the antarctic bacterium alteromonas haloplanctis. | the thermal stability of the cold-active alpha-amylase (aha) secreted by the antarctic bacterium alteromonas haloplanctis has been investigated by intrinsic fluorescence, circular dichroism, and differential scanning calorimetry. it was found that this heat-labile enzyme is the largest known multidomain protein exhibiting a reversible two-state unfolding, as demonstrated by the recovery of deltahcal values after consecutive calorimetric transitions, a deltahcal/deltaheff ratio close to unity, an ... | 1999 | 10194383 |
| alteromonas prolidase for organophosphorus g-agent decontamination. | enzymes catalyzing the hydrolysis of highly toxic organophosphorus compounds (ops) are classified as organophosphorus acid anhydrolases (opaa; ec 3.1.8.2). recently, the genes encoding opaa from two species of alteromonas were cloned and sequenced. sequence and biochemical analyses of the cloned genes and enzymes have established alteromonas opaas to be prolidases (e.c. 3.4.13.9), a type of dipeptidase hydrolyzing dipeptides with a prolyl residue in the carboxyl-terminal position (x-pro). altero ... | 1999 | 10421483 |
| idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the pacific ocean, including descriptions of two species, idiomarina abyssalis sp. nov. and idiomarina zobellii sp. nov. | two bacterial strains, kmm 227t and 231t, were isolated from seawater samples collected from the north-western pacific ocean at a depth of 4000-5000 m and were characterized using polyphasic taxonomy. both were gram-negative, psychrotolerant, heterotrophic, aerobic and required nacl for growth (0.6-15.0%). the temperature for growth was 4-30 degrees c. both strains were rod-shaped, with a single flagellum. however, strain kmm 231t revealed a single long fimbrium. cellular fatty acids detected in ... | 2000 | 10758902 |
| bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rrna gene restriction analysis and sequence analysis of 16s rrna genes. | the nearly universal colonization of surfaces in marine waters by bacteria and the formation of biofilms and biofouling communities have important implications for ecological function and industrial processes. however, the dynamics of surface attachment and colonization in situ, particularly during the early stages of biofilm establishment, are not well understood. experimental surfaces that differed in their degrees of hydrophilicity or hydrophobicity were incubated in a salt marsh estuary tida ... | 2000 | 10653705 |
| substrate and stereochemical specificity of the organophosphorus acid anhydrolase from alteromonas sp. jd6.5 toward p-nitrophenyl phosphotriesters. | the enzyme opaa hydrolyzes p-nitrophenyl phosphotriesters bearing substituents at the phosphorus center ranging in size from methyl to phenyl. the enzyme exhibits stereoselectivity toward the hydrolysis of chiral substrates with a preference for the sp enantiomer. | 2000 | 10866401 |
| culturability and in situ abundance of pelagic bacteria from the north sea. | the culturability of abundant members of the domain bacteria in north sea bacterioplankton was investigated by a combination of various cultivation strategies and cultivation-independent 16s rrna-based techniques. we retrieved 16s rrna gene (rdna) clones from environmental dnas and determined the in situ abundance of different groups and genera by fluorescence in situ hybridization (fish). a culture collection of 145 strains was established by plating on oligotrophic medium. isolates were screen ... | 2000 | 10877804 |
| reclassification of alteromonas distincta romanenko et al. 1995 as pseudoalteromonas distincta comb. nov. | the 16s rrna gene of alteromonas distincta kmm 638t was amplified, cloned and sequenced. the nucleotide sequence was aligned with sequences of representative strains of alteromonas, moritella, pseudoalteromonas and shewanella. results of phylogenetic analysis, using neighbour-joining and fitch-margoliash methods, clearly indicated that this species should be assigned to the genus pseudoalteromonas. on the basis of polyphasic data obtained from previous work and this study, it is proposed that th ... | 2000 | 10826797 |
| assignment of alteromonas elyakovii kmm 162t and five strains isolated from spot-wounded fronds of laminaria japonica to pseudoalteromonas elyakovii comb. nov. and the extended description of the species. | a marine bacterium, alteromonas elyakovii kmm 162t, which was described recently, and five strains isolated from spot-wounded fronds of laminaria japonica have been subjected to phylogenetic analysis, and geno- and phenotypic characterization. the phenotypic features of pseudoalteromonas elyakovii strains were closely related to that of pseudoalteromonas espejiana iam 12640t, but utilization of three carbon compounds (d-mannose, l-tyrosine and trehalose) distinguished both species. the g+c conte ... | 2000 | 10826813 |
| cloning and sequencing of the gene encoding an aldehyde dehydrogenase that is induced by growing alteromonas sp. strain ke10 in a low concentration of organic nutrients. | the protein composition of alteromonas sp. strain ke10 cultured at two different organic-nutrient concentrations was determined by using two-dimensional polyacrylamide gel electrophoresis. the cellular levels of three proteins, olga, -b, and -c, were considerably higher in cells grown in a low concentration of organic nutrient medium (lon medium; 0.2 mg of carbon per liter) than cells grown in a high concentration of organic nutrient medium (hon; 200 mg of c liter(-1)) or cells starved for organ ... | 2000 | 10788355 |
| successional changes in the genetic diversity of a marine bacterial assemblage during confinement. | the successional changes in the genetic diversity of mediterranean bacterioplankton subjected to confinement were studied in an experimental 300 1 seawater enclosure. five samples were taken at different times and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) fingerprinting to rapidly monitor changes in the bacterial genetic diversity. dgge analysis clearly showed variations between the samples. three of the five samples, with different dgge banding pat ... | 2000 | 10795685 |
| purification and characterization of n-acetylglucosamine-6-phosphate deacetylase from a psychrotrophic marine bacterium, alteromonas species. | a psychrotrophic bacterium, strain mct-9, which produced an n-acetylglucosamine-6-phosphate deacetylase, was isolated from a deep-seawater sample in the mariana trough. the mct-9 strain was identified as alteromonas sp. the native enzyme had a molecular mass of 164,000 da, and was predicted to be composed of four identical subunits with molecular masses of 41,000 da. the purified enzyme hydrolyzed n-acetylglucosamine (glcnac), glcnac-6-phosphate, and glcnac-6-sulfate. considering the low k(m) an ... | 2000 | 10804240 |
| cloning, nucleotide sequence and expression of the beta-n-acetylglucosaminidase gene from aeromonas sp. no. 10s-24. | the beta-glcnacase gene of aeromonas sp. no. 10s-24 was cloned and sequenced. the gene consisted of 2505 bp encoding 835 amino acid residues. the amino acid sequence of the gene shares sequence homology with beta-glcnacases of vibrio vulnificus (37.4%), serratia marcescens (36.4%), v. harveyi (36.4%), v. parahemolyticus (33.5%), alteromonas sp. strain o-7 (27.0%), and candida albicans (28.6%). the beta-glcnacase gene of aeromonas sp. no. 10s-24 was about 630-fold overexpressed in escherichia col ... | 2000 | 16232720 |
| [recent advances in tetrodotoxin research]. | one century has passed since fugu toxin was named tetrodotoxin (ttx) by tahara. chemical problems such as crystallization of tetrodotoxin and subsequent structure determination were solved by research groups headed by tsuda, hirata, woodward, and mosher. the international symposium on the chemistry of natural products in kyoto (1964) was well known as symposium which the structure of ttx was internationally clarified. since the first isolation of toxin from taricha torosa (imori) as natural sour ... | 2000 | 11082696 |
| succession of pelagic marine bacteria during enrichment: a close look at cultivation-induced shifts. | enrichment experiments with north sea bacterioplankton were performed to test if rapid incubation-induced changes in community structure explain the frequent isolation of members of a few particular bacterial lineages or if readily culturable bacteria are common in the plankton but in a state of dormancy. a metabolic inhibitor of cell division (nalidixic acid [na]) was added to substrate-amended (s+) and unamended (s-) grazer-free seawater samples, and shifts in community composition and per cel ... | 2000 | 11055904 |
| alteration of a single tryptophan residue of the cellulose-binding domain blocks secretion of the erwinia chrysanthemi cel5 cellulase (ex-egz) via the type ii system. | cel5 (formerly known as endoglucanase z) of erwinia chrysanthemi is secreted by the out type ii pathway. previous studies have shown that the catalytic domain (cd), linker region (lr) and cellulose-binding domain (cbd) each contain information needed for secretion. the aim of this work was to further investigate the secretion-related information present in the cbd(cel5). firstly(, )deleting a surface-exposed flexible loop had no effect on secretion. this indicated that some structural freedom is ... | 2000 | 11023779 |
| differentiation of chitinase-active and non-chitinase-active subpopulations of a marine bacterium during chitin degradation. | the ability of marine bacteria to adhere to detrital particulate organic matter and rapidly switch on metabolic genes in an effort to reproduce is an important response for bacterial survival in the pelagic marine environment. the goal of this investigation was to evaluate the relationship between chitinolytic gene expression and extracellular chitinase activity in individual cells of the marine bacterium pseudoalteromonas sp. strain s91 attached to solid chitin. a green fluorescent protein repo ... | 2000 | 10919822 |
| metabolites from a marine bacterium pseudomonas/alteromonas, associated with the sponge dysidea fragilis. | the lipophylic extract from a black sea bacterium, associated with the sponge dysidea fragilis, was investigated. saturated hydrocarbons and fatty acids of the lipids were identified. the concentrations of the polycyclic compounds appeared to be negligible. the main components appeared to be phosphatidyl ethanolamine, followed by phosphatidyl serine. the first was investigated by fab mass spectrometry and a series of molecular species partially identified. | 2000 | 11007181 |
| the alpha-amylase gene amyh of the moderate halophile halomonas meridiana: cloning and molecular characterization. | two types of tn1732-induced mutants defective in extracellular amylase activity were isolated from the moderate halophile halomonas meridiana dsm 5425. type i mutants displayed amylase activity in the periplasm, and were unable to use any of the carbon sources tested, including starch and its hydrolysis product maltose. the type ii mutant was affected in the gene responsible for the synthesis of the extracellular alpha-amylase. this gene (amyh) was isolated by functional complementation of mutan ... | 2000 | 10784044 |
| expression, purification, crystallization and preliminary x-ray analysis of the iota-carrageenase from alteromonas fortis. | this is the first crystallization report of a glycoside hydrolase which belongs to family 82. a recombinant form of his-tagged iota-carrageenase from alteromonas fortis was expressed, purified and crystallized. crystals were obtained by the vapour-diffusion method using polyethylene glycol (m(w) = 6000) as a precipitant. they belong to space group p2(1), with unit-cell parameters a = 56. 75, b = 91.04, c = 125.01 a, beta = 93.41 degrees. the unit cell contains two molecules in the asymmetric uni ... | 2000 | 10818359 |
| a serine protease-encoding gene (aprii) of alteromonas sp. strain o-7 is regulated by the iron uptake regulator (fur) protein. | the ferric uptake regulator (fur) box-like sequence was located upstream of the serine protease-encoding gene (aprii) from a marine bacterium, alteromonas sp. strain o-7. to clarify whether the production of aprii (the gene product of aprii) is regulated by the environmental iron concentrations, this strain was cultured under iron-depleted or iron-rich conditions and the level of aprii in the culture supernatant was analyzed by western blotting. the production of aprii was significantly represse ... | 2000 | 10966390 |