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comparative in vitro activity of ciprofloxacin against campylobacter spp. and other bacterial enteric pathogens.a comparison was made of the in vitro activity of ciprofloxacin (bay o 9867) with nine other antibiotics against isolates of campylobacter jejuni, salmonella spp., shigella spp., yersinia enterocolitica, clostridium difficile, vibrio spp., and escherichia coli. minimum inhibitory concentrations of ciprofloxacin were the lowest of any compound tested for all organisms except c. difficile.19846732220
osteitis of the foot due to campylobacter jejuni. 19846699418
dna relatedness among strains of campylobacter jejuni and campylobacter coli with divergent serogroup and hippurate reactions.eleven strains of campylobacter from earlier fluorescent-antibody studies were examined by dna hybridization to determine their species. three of the strains hydrolyzed sodium hippurate, and eight did not. four of the hippurate-negative strains were in campylobacter jejuni serogroups, and the remaining strains were in both c. jejuni and campylobacter coli serogroups. dna relatedness to type strains of c. jejuni and c. coli indicated that all three of the hippurate-positive strains and two of the ...19846746886
occurrence of campylobacter jejuni in country chicken in calcutta. 19846746032
[campylobacter jejuni gastroenteritis].acute gastroenteritis is a major cause of hospital admission with attendant morbidity and occasional mortality. campylobacter jejuni recently has been recognized as a common cause of acute bacterial gastroenteritis in children and adults. its frequency of isolation is comparable to and in many studies exceeds that of salmonella isolations from diarrheal stools of hospitalized patients. medical workers in many parts of the world confirmed that campylobacter jejuni was found more commonly in feces ...19846748977
studies of campylobacter jejuni in patients with inflammatory bowel disease.cultures, serology, and immunohistochemical tests for campylobacter jejuni were performed on 74 patients with inflammatory bowel disease of various disease activity and in healthy and diseased control populations. fecal cultures were negative in all groups tested. antibodies to c. jejuni were assessed both by a complement fixation assay and an enzyme-linked immunosorbent assay to multiple serotypes of the organism. antibody titers in inflammatory bowel disease patients and control populations we ...19846689672
a study of the spread of campylobacter jejuni in four large kitchens.campylobacters were sought in swabs taken from work surfaces, sinks and floors of four kitchens-i.e. hospital, university, cook-freeze and commercial, processing frozen or fresh chickens. each kitchen was visited on four occasions. in the large commercial kitchen environmental contamination was found on each visit, whereas campylobacters were isolated on six of the twelve visits to the other kitchens. the hands of operatives were contaminated with campylobacters on only two of the 45 swabs taken ...19846736643
isolation of campylobacter jejuni in lagos-nigeria a new bacterial agent of diarrhoea. 19846745142
properties of crude campylobacter jejuni heat-labile enterotoxin.the amount of crude campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure escherichia coli heat-labile toxin (lt) in the chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used gm ganglioside or antisera to lt or both. maximum c. jejuni enterotoxin production was achieved by growth at 42 degrees c for 24 h under agitation in supplemented gc medium. adding polymyxin separately to eithe ...19846746090
systematic investigation of enrichment media for wild-type campylobacter jejuni strains.of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of campylobacter jejuni from cecal contents of chickens and chicken livers. it allowed the retrieval of 1 cfu as did solid media but permitted the screening of 50-times larger volumes. double-strength enrichment medium required 5 to 10 cfu for growth. omission of lauryl sulfate reduced the ...19846746890
antibiotic sensitivity patterns of campylobacter jejuni/coli isolated from laboratory animals and pets.fifty-four strains of campylobacter jejuni/coli isolated from a variety of species of laboratory animals as well as pet dogs were tested by an agar dilution technique for susceptibility to each of 12 antimicrobial agents. gentamicin and furazolidone were the most active of the drugs examined. the strains tested frequently were sensitive to two other aminoglycoside antibiotics, neomycin and kanamycin. erythromycin also was effective at levels achievable in serum except in three strains which were ...19846748605
comparative studies on competitive exclusion of three isolates of campylobacter fetus subsp. jejuni in chickens by native gut microflora.resistance of young chicks to campylobacter fetus subsp. jejuni was substantially increased by early exposure to native gut microflora. protection was demonstrated against two human isolates and a chicken isolate of c. fetus subsp. jejuni. significant protection against the chicken isolate was observed throughout a 91-day test period. infection reached 100% (25/25) in the untreated group at 56 days of age and only 4% (1/25) in the group treated with native gut microflora. campylobacter fetus sub ...19846721792
campylobacter jejuni infections in haifa subdistrict, israel, summer 1981.a study investigating the clinical and epidemiological characteristics of campylobacter jejuni infections in an urban community in israel is presented. most of the information was obtained by interviewing 76 patients who constituted a systematic sample out of a total of 215 patients suffering from acute campylobacter jejuni diarrhoea during the summer of 1981. the crude annual incidence rate was 17 per 10 000. age-specific incidence rate in infants up to one year of age was eight times higher th ...19846735568
advantage of using enrichment-culture techniques to isolate campylobacter jejuni from stools. 19846715907
campylobacter jejuni associated diarrhea in commercially reared beagles.campylobacter jejuni was isolated from nine of ten (90%) juvenile beagles with diarrhea, compared with an isolation rate of five of eight (63%) from beagles which remained asymptomatic during the 2 month study. in four dogs, the diarrhea was recurrent and characterized by watery, mucoid, bile-streaked feces with occasional blood. elevated temperature and leukocytosis were noted in three of these dogs. two dogs with diarrhea also had giardia canis trophozoites.19846727288
the ability of campylobacter media supplements to neutralize photochemically induced toxicity and hydrogen peroxide.nutrient agar plates stored in light and air for 48 h became inhibitory for campylobacter jejuni, c. coli and nalidixic acid-resistant, thermophilic campylobacter (nartc) strains. all five campylobacter test strains showed greater than 5 log reduction in counts on media which had been stored in light and air. media stored in the dark and/or in a reduced atmosphere did not become inhibitory and supported the growth of campylobacters. ferrous sulphate, sodium pyruvate, blood, charcoal or sodium me ...19846706882
abortion in the dog due to campylobacter species.campylobacter jejuni was isolated from a wheaten terrier bitch which had aborted (in southern idaho). a campylobacter of undetermined species also was isolated from aborted poodle pups (in central washington). it appears that persons may have been the source of infection in the idaho event, but the source for the other was not speculated. these findings of perinatal death due to campylobacter in dogs from widely separated areas indicate that pathologic evaluation of cases involving premature and ...19846711985
association of campylobacter jejuni with laying hens and eggs.laying hens were individually caged at 20 weeks of age and tested for fecal excretion of campylobacter jejuni (minimum level of detection was 100 cfu/g) during a 42-week period. peak rates of c. jejuni isolation (approximately 25% of hens positive) occurred at two different times, in october and in late april to early may. before being segregated in late september, birds were allowed to consume fecal matter, litter, and communal drinking water, all likely sources of c. jejuni. the increased excr ...19846712220
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces? 198416811148
isolation of campylobacter jejuni from patients with diarrhea. 198416789269
aetiological forms.at least 25 etiologic agents have been implicated in acute diarrhea. in children, the most common are the rotaviruses, enterotoxigenic escherichia coli, campylobacter jejuni, salmonallae, and shigellae. for example, an attempt was made to isolate enteropathogenic micro-organisms in children under 2 years of age hospitalized in ethiopia with severe or moderate dehydration. a causal agent was identified in 70% of the 175 cases analyzed. 49% of these cases involved rotavirus infections, 19% inc ...198512314104
serotyping of campylobacter jejuni from an outbreak of enteritis implicating chicken.an outbreak of campylobacter enteritis involving 7 of 17 people over a period of 5 days followed a dinner at a restaurant. a chicken casserole dish was implicated with a food-specific attack rate of 58%. campylobacter jejuni penner serotype 18/21/29, resistant to metronidazole, was isolated from 3 of 4 symptomatic patients and from three raw fresh chicken samples closely associated with the implicated chicken. numbers of c. jejuni in the chicken ranged from 5.3 x 10(1) to 7.5 x 10(2) colony form ...19854056451
[occurrence and transmission of campylobacter jejuni/coli in young poultry fattening production. 1]. 19853910389
campylobacter infection in domestic dogs.campylobacter jejuni, a recently recognised human enteric pathogen, was recovered from the faeces of 21.7 per cent of domestic dogs with diarrhoea as compared with only 3.1 per cent of normal healthy dogs. the recovery rate from non-diarrhoeic, but unhealthy, domestic dogs was 6.7 per cent. the differences in the incidence of c jejuni between breeds was not statistically significant. the majority of isolations were from puppies between birth and six months old. the incidence of c jejuni in domes ...19854002595
investigations on the role of flagella in the colonization of infant mice with campylobacter jejuni and attachment of campylobacter jejuni to human epithelial cell lines.the biochemical and biological properties of the flagella of campylobacter jejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): a flagellate, non-motile variant (sf-1) and an aflagellate variant (sf-2). phenotypic and biochemical analysis of the strains and amino acid analysis of the isolated flagella suggest that the variants differed from the wild-type strain only in the absence of flagella and/or motility. the aflagellate variant ...19854067286
[seasonal distribution of campylobacter jejuni and campylobacter coli (1 september 1982--31 august 1983)]. 19854068889
[bacteriological and serological evidence for the presence of campylobacter jejuni in poultry, animals and man (1982-1983)]. 19854070879
campylobacter jejuni-associated hemolytic-uremic syndrome: a possible role for endotoxin. 19853977526
inhibition of campylobacter coli and campylobacter jejuni by antibiotics used in selective growth media.the ability of campylobacter coli and campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. mic data for c. coli indicated that some strains were more susceptible to the antibiotics than were the c. jejuni strains tested. a reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. only one of nine of the antibiotic combinations studied did not markedl ...19854077962
[campylobacter jejuni infections: epidemiology and possibility of prevention]. 19854079992
evidence of udder excretion of campylobacter jejuni as the cause of milk-borne campylobacter outbreak.we describe a community outbreak of campylobacter enteritis associated with the consumption of untreated milk, apparently contaminated by two cows with campylobacter mastitis. the outbreak occurred in two phases. strains of campylobacter jejuni of the penner serogroup complex 4, 13, 16, 50 and preston biotype code 6100 were isolated from patients in both episodes and from the faeces of the cattle, milk filters, bulk milk and retail milk. milk samples from two of 40 milking cows were found to con ...19853989284
serotyping of and hippurate hydrolysis by campylobacter jejuni isolates from human patients, poultry and pigs in the netherlands.three hundred strains of campylobacter jejuni, isolated from human patients, poultry and pigs were serotyped according to the penner-lauwers system and furthermore tested for hippurate hydrolysis. serotyping showed a close relationship between human and chicken strains, whereas there was little relationship between human and pig strains. a similar conclusion was drawn from the results of the hippurate hydrolysis test: 86% of human and 94% of poultry strains were positive, whereas 91% of pig stra ...19854004203
severe campylobacter infection in children.children suffering from campylobacter jejuni infection and who were admitted to children's hospitals in bristol and newcastle over a period of 2 years are reviewed. gastro-intestinal disease caused by c. jejuni was as common as that due to salmonella spp. three neonates suffered particularly severe infections which resembled necrotising enterocolitis. one such case is described.19854086864
intestinal parasites and bacteria in aboriginal children in south west australia.in a study of intestinal parasites in 697 aboriginal children under the age of 6 years in south-west australia, giardia lamblia was recorded in 26% and hymenolepis nana in 13.9%. g. lamblia infections occurred above 4 months of age, and h. nana infections above 18 months of age. there was a close correlation between infection with these two species (chi 2, p less than 0.001). campylobacter jejuni, the predominant bacterial species recorded, was isolated in 2.3%, and was related to bowel symptoms ...19853977790
occurrence of campylobacter jejuni in diarrhoeic and non-diarrhoeic children in são paulo, brazil. 19854089456
susceptibility of campylobacter jejuni and campylobacter coli to macrolides and related compounds.the susceptibility of 105 thermophilic campylobacters from human and swine origins to eight macrolides and related compounds was tested. erythromycin, josamycin, clindamycin, and ase 136 bs (a new erythromycin derivative) were the most active against the human strains. the swine strains were highly resistant, except to pristinamycin. the human campylobacter coli strains (except for two strains) behaved like the c. jejuni strains.19854091531
phagocytosis of campylobacter jejuni and its intracellular survival in mononuclear phagocytes.in vitro phagocytosis and intracellular survival of campylobacter jejuni strain 2964 in mononuclear phagocytes were studied. the following three types of mononuclear phagocytes were used: a j774g8 peritoneal macrophage line derived from balb/c mice, resident balb/c peritoneal macrophages, and human peripheral blood monocytes. when c. jejuni and mononuclear phagocytes were combined at a ratio of 75:1, light microscopy, fluorescent microscopy, and electron microscopy all indicated that c. jejuni c ...19853988342
comparison of enrichment broths for isolation of campylobacter jejuni.growth of campylobacter jejuni was compared in enrichment broths of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) and park et al. (c. e. park, z. k. stankiewicz, j. lovett, and j. hunt, can. j. microbiol. 27:841-842, 1981), as modified by lovett et al. (j. lovett, d. w. francis, and j. m. hunt, appl. environ. microbiol. 46:459-462, 1983). inoculated foods used were cream-pudding types, which may be cross-contaminated by improper handling, improper storage of meats prepared simul ...19854091559
fatalities associated with campylobacter jejuni infections.although campylobacter jejuni is now recognized as a common cause of gastroenteritis, fatalities associated with this infection in the united states have not been previously reported. two fatalities associated with c jejuni infections occurred over a two-year period in the denver metropolitan area. the first case was in a previously healthy 26-year-old woman who died following a two-day diarrheal illness. the second case was in a 69-year-old diabetic woman who died 19 hours after developing a ga ...19853989964
presence of zoonotic pathogens (yersinia spp., campylobacter jejuni, salmonella spp., and leptospira spp.) simultaneously in dogs and cats.the purpose of this study was to determine the presence of zoonotic pathogens simultaneously in animals. the isolation of human pathogenic yersinia enterocolitica (ye), yersinia pseudotuberculosis (yp), campylobacter jejuni (cj), salmonella spp. (sal) and leptospira spp. (lep) in 318 cats and 252 dogs were performed in shimane prefecture, japan. a total of 13 isolates of yp (4 strains) and sal (9 strains) were recovered from intestine and/or mesenteric lymph nodes (mln) of 13 cats (4.1%) but not ...19854096152
acute polyneuritis with cranial nerve involvement following campylobacter jejuni infection. 19854009198
serotyping of campylobacter jejuni isolated from sporadic cases and outbreaks in northern norway.in the northern regions of norway, 123 cases of campylobacter jejuni gastroenteritis were either sporadic infections or due to five separate outbreaks during 1980-83. using a serotyping scheme based on thermostable antigens and the passive haemagglutination technique, 92 isolates were serotyped. excluding duplicates from the outbreaks, the serotype distribution based on 79 isolates showed that serotypes 6, 7 and 12, 40 were the most frequent in occurrence. each included 11 (13.9%) of the isolate ...19854013744
[spontaneous peritonitis caused by campylobacter jejuni in a cirrhotic patient]. 19853990397
comparison of gauze swabs and membrane filters for isolation of campylobacter spp. from surface water.the epidemiology of campylobacter jejuni indicates that waterborne transmission is important; the organism has been isolated from seawater, fresh water, and estuarine sites. membrane filtration, with and without use of an enrichment broth, has been the most common method for isolating c. jejuni from water. we evaluated two methods for isolating c. jejuni from water: membrane filtration and gauze filtration. the membrane filters evaluated included 0.22- and 0.45-micron-pore millipore filters (mil ...19854073894
laboratory infection of chicken eggs with campylobacter jejuni by using temperature or pressure differentials.fertile chicken eggs were infected in our laboratory with campylobacter jejuni suspensions by using temperature or pressure differential methods of inoculation. after 2 days of incubation, over 90% of the eggs carried c. jejuni when iron was present in the inoculum. this percentage declined rapidly until by day 8, less than 10% of the eggs were detectably infected. however, up to 11% of hatched, healthy chicks carried c. jejuni in their intestinal tracts. the isolated organisms were of the same ...19854015086
biotypes and serotypes of thermophilic campylobacters isolated from cattle, sheep and pig offal and other red meats.in this study we examined 730 faecal samples of offal (mainly liver), mince-meat and sausage meat collected from abattoirs and retail butchers' shops for campylobacters. campylobacter jejuni or c. coli were isolated from 30.6, 10.5 and 6% of sheep, cattle and pig offal samples respectively. specimens collected from abattoirs were, in general, more often contaminated than material obtained from retail butchers' shops. only 1.4% of minced meats and sausage meats contained campylobacters. most isol ...19854020105
alterations of myoelectric activity associated with campylobacter jejuni and its cell-free filtrate in the small intestine of rabbits.we evaluated the effects of a culture of campylobacter jejuni and its cell-free filtrate on myoelectric activity of isolated ileal segments in new zealand white rabbits. hematoxylin and eosin staining and scanning electron microscopy were used to assess the association between histologic changes and alterations in intestinal myoelectric activity. a culture of c. jejuni was shown to cause a significant increase in repetitive bursts of action potentials (rbaps) (6.9 +/- 1.2 rbap/h; p less than 0.0 ...19854007424
transmission of campylobacter jejuni by the housefly (musca domestica).houseflies (musca domestica) were infected with campylobacter jejuni after being confined for 5 days in a horsfall isolator containing 25-day-old chickens known to be fecal excretors of the organism. contaminated flies, when subsequently transferred to a second unit, transmitted c. jejuni to specific-pathogen-free chickens. allowing a sample of 32 houseflies to ingest c. jejuni in a liquid suspension resulted in recovery rates of 20% from the feet and ventral surface of the body and 70% from the ...19854026732
campylobacter jejuni isolated from a rectal biopsy specimen. 19853989330
reversible expression of flagella in campylobacter jejuni.campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype.19854066041
epidemiologic and laboratory investigation of an outbreak of campylobacter enteritis associated with raw milk.an outbreak of gastroenteritis occurred in march-april 1981, in wichita, kansas, and involved more than 250 persons who drank raw milk from a single local dairy. campylobacter jejuni was recovered from 60 of 116 (52%) persons in households that had one or more ill family members. a cohort study of families that belonged to a food cooperative that purchased raw milk from the implicated dairy showed a significant association between illness and having drunk raw milk. thirty-nine of 55 (71%) person ...19854050775
motility as an intestinal colonization factor for campylobacter jejuni.the colonization of the intestinal tract of suckling mice by campylobacter jejuni was examined by orally challenging the mice with a wild-type strain and several nonmotile mutant strains which were isolated after treating the wild-type strain with mutagens. the wild-type strain had colonized the lower portion of the small intestine, the caecum and the colon 2 d after inoculation. two nonmotile strains, one of which (m8) had lost all the flagellar structure including the filament, the hook and th ...19854056739
chemically defined media for auxotyping of campylobacter jejuni.a set of chemically defined media has been developed for the cultivation of campylobacter jejuni strains of human origin. a minimal medium, a complete medium and 5 different nutrient-deficient media (ndm1-ndm5) are described. some of the strains investigated required l-methionine(lacking in ndm1), l-cystine and l-cysteine (ndm2), k2hpo4 (ndm 3), kh2po4 (ndm4) and nad, thiamine and calcium pantothenate (ndm5). 57.7% of the strains investigated required l-methionine. the strains grew at ph 6.6-7.7 ...19854060921
role of litter in the transmission of campylobacter jejuni.autoclaved or non-autoclaved used broiler litter that was experimentally contaminated with campylobacter jejuni was capable of infecting specific-pathogen-free chicks maintained in modified horsfall isolators. artificially infected chicks became fecal shedders of c. jejuni, resulting in contamination of both autoclaved and non-autoclaved used broiler litter. fecal shedding of c. jejuni by litter-reared, artificially infected chicks persisted for at least 63 days after chicks were transferred to ...19854026733
campylobacter colitis: histological immunohistochemical and ultrastructural findings.the colonic biopsy specimens of 22 patients with colitis and positive stool cultures for campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate campylobacter colitis from chronic inflammatory bowel disease. in addition we tried to identify campylobacter inclusions by means of immunohistochemistry and electron microscopy as evidence for invasion of the colonic mucosa. the results show that the majority of patients with campylobacter col ...19854029720
experimental infection of hamsters with campylobacter jejuni.campylobacter jejuni is a major cause of diarrhea and enterocolitis in humans and animals. a mammalian model has been developed for studying pathogenesis of the disease in hamsters by colonizing the ileum and cecum with c. jejuni via either oral intubation after purgation or direct surgical inoculation into the cecum. this colonization resulted in production of disease including diarrhea and intestinal lesions that resembles enterocolitis due to c. jejuni in humans and domestic animals. the muco ...19854038721
hypogammaglobulinemia and recurrent campylobacter jejuni infection. 19854045252
in vitro susceptibilities of campylobacter-like organisms to twenty antimicrobial agents.we determined mics of 20 antimicrobial agents for 50 representative strains of four subgroups of campylobacter-like organisms (clos) by agar dilution. ampicillin, gentamicin, doxycycline, tetracycline, ceftriaxone, rifampin, spectinomycin, nalidixic acid, and chloramphenicol were active against all strains of clos. most clo strains (83%) were inhibited by 4 micrograms of sulfamethoxazole per ml and by 8 micrograms of trimethoprim-sulfamethoxazole per ml. of type 1 strains, 28% were resistant to ...19853834828
campylobacter enteritis and bloody stools in the neonate.within 72 hours of birth three babies had loose stools containing fresh blood, mucus, and campylobacter jejuni/coli. campylobacter enteritis should be considered in newborn babies passing blood per rectum.19853874603
etiology of acute infectious diarrhea in a highly industrialized area of switzerland.during an 18-mo period between 1981 and 1982, a prospective study was conducted in 119 adult patients with acute diarrhea. a diarrhea-inducing microorganism or toxin could be identified in 38.7% of the patients. salmonella sp and campylobacter jejuni were the leading agents that caused diarrheal illness in 25% of the investigated population. clostridium difficile was found in 6%, mainly after previous antibiotic therapy. rotavirus was rarely isolated and enterotoxigenic escherichia coli were not ...19853964774
campylobacter jejuni associated with acute relapse and abscess formation in crohn's disease. 19853831348
[intestinal campylobacter jejuni infection in chilean infants]. 19853834562
susceptibility of campylobacter isolates to the bactericidal activity of human serum.although campylobacter jejuni and related thermophilic organisms are more common human pathogens than are campylobacter fetus, most bloodstream or systemic isolates are c. fetus. to understand the pathophysiology related to this observation, the authors studied susceptibility to the bactericidal activity of normal human serum of campylobacter coli, c. jejuni, and c. fetus isolates from feces and blood. in standardized assays, 10 of 15 c. jejuni and related isolates showed 90% kill (mean, 90.6% + ...19853968449
[the behavior of campylobacter jejuni in various foodstuffs].for the purpose of ascertaining the survival time of campylobacter jejuni in foodstuffs under differing conditions, milk, ice-cream, meat salad and drinking water were inoculated with a defined quantity of campylobacter jejuni and subsequently the number of colony-forming units was determined at first at 12-hour intervals and later once a day. it was found that the survival time in milk at +5 degrees c was 15 days, in drinking water 6 days, but only 7 and 4 days respectively at 22 degrees c. in ...19853839617
illness associated with campylobacter laridis, a newly recognized campylobacter species.campylobacter laridis, a recently described thermophilic campylobacter species found principally in seagulls, has not previously been linked to illness in humans. six clinical isolates of this species were referred to the national campylobacter reference laboratory in 1982 and 1983. each isolate was confirmed by biochemical characterization and by dna relatedness studies. the six isolates were obtained during an illness: enteritis in four, severe crampy abdominal pain in one, and terminal bacter ...19853972989
comparison of basal media for culturing campylobacter jejuni and campylobacter coli.four strains of campylobacter jejuni and four strains of campylobacter coli were used to compare the quantitative growth of campylobacter cells on blood agar base no. 2 (oxoid), brucella agar (bbl microbiology systems and difco laboratories), campylobacter agar base (difco), columbia blood agar base (difco and oxoid), and mueller-hinton agar (difco and oxoid). columbia blood agar base and blood agar base no. 2 were inhibitory to most of the strains tested, as evidenced by reduced (10- to 1,000-f ...19853972990
lipopolysaccharide characteristics of pathogenic campylobacters.most campylobacter jejuni strains are sensitive and most campylobacter fetus strains are resistant to the bactericidal activity in normal human serum. we purified lipopolysaccharides from campylobacter strains to determine whether their composition and structure relate to serum susceptibility. the lipopolysaccharide of two serum-sensitive strains was best isolated by the galanos procedure, but for two serum-resistant strains a cold-ethanol extraction was optimal. for each lipopolysaccharide prep ...19853967920
a one-year survey of campylobacter enteritis and other forms of bacterial diarrhoea in hong kong.the following enteropathogens were isolated from the faeces of 769 (10.2%) of 7,545 patients of whom 5,704 had diarrhoea or abdominal pain, attending a teaching hospital in hong kong during one year: salmonellae 458 (6.1%); vibrio parahaemolyticus 125 (1.7%); campylobacters 108 (1.4%); shigellae 83 (1.1%); others 19 (0.3%). further identification of the campylobacter isolates showed that 63 (58%) were campylobacter jejuni biotype 1, 44 (41%) were c. coli and only one was c. jejuni biotype 2. sev ...19853973381
appearance in patients' sera of antibodies against purified antigen of campylobacter jejuni and its relation to the bacterium-excreting period. 19853973420
the prevalence of campylobacter jejuni in random-source cats used in biomedical research. 19853973421
campylobacter jejuni mastitis in a cow: a zoonosis-related incident. 19853976151
prevalence of campylobacter jejuni and salmonella during pig slaughtering.it was found that 79% of healthy pigs, slaughtered in three different slaughterhouses in the netherlands, were intestinal carriers of campylobacter jejuni (mean number 4000 cfu per g), and 21% of the same pigs had salmonella in the intestinal tract (mean number 10 cfu per g). immediately after slaughter, campylobacter was swabbed from 9% of the carcasses and salmonella from 13%. it is concluded from these data that most of the contamination on carcasses does not originate directly from the intes ...19853976157
[isolation of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter swine depending on the season]. 19853976311
[enteric infection due to campylobacter jejuni]. 19853930026
[campylobacter jejuni infections in children].in a two-year-study, the primary feces of all children with gastroenteritis or acute abdominal symptoms were checked for the presence of campylobacter jejuni (c. j.) even when there was no diarrhea. c. j. could be isolated 19 times from among 974 fecal samples. the most frequent admission diagnosis after gastroenteritis was the suspicion of appendicitis. in the forefront of the clinical picture was abdominal pain, which radiated into the right lower abdomen especially in children of school age, ...19853895753
in vitro blastogenic response and immunoglobulin synthesis in murine spleen cells stimulated with lipopolysaccharides from campylobacter jejuni and campylobacter coli.lipopolysaccharides isolated from strains of campylobacter jejuni and campylobacter coli were mitogenic for spleen cells as measured by 3h-thymidine incorporation. the incorporation was dose dependent with an increase with increased concentration of lps. addition of lps beyond 500 micrograms/ml gave inhibition of incorporation. stimulation of the spleen cells for 3 days with lps led to a polyclonal activation of immunoglobulin synthesis. the amount of immunoglobulins synthesised showed a maximum ...19853909739
use of auxotyping for epidemiological studies of campylobacter jejuni and campylobacter coli infections.a chemically defined medium developed for neisseria gonorrhoeae was modified to support the growth of campylobacter jejuni and campylobacter coli. a total of 76 isolates of c. jejuni and 14 isolates of c. coli were tested on this medium, which was designated campylobacter defined medium (cdm), over a 3-month period. although none of the c. coli isolates appeared to require amino acids, 51% of the c. jejuni tested required one and 7% required multiple amino acids for growth. an analysis of isolat ...19853988340
studies on the identification of campylobacter species using biochemical tests and high-performance liquid chromatography.a total of 56 strains of campylobacter jejuni (c. jejuni) isolated from diarrhoeal patients were characterized by biochemical tests. the following reactions were performed: hydrolysis of hippurate, reduction of nitrate and nitrite, activity of deoxyribonuclease, hydrolysis of tween(r) 40, 60 and 80. including the hydrolysis of the different tweens(r), 14 biotypes could be distinguished. 19 out of the 56 strains of c. jejuni and the nomenclatural type strains of c. jejuni, c. coli, c. laridis, c. ...19853911663
[season-related incidence of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter cattle]. 19853993275
estimation of campylobacter spp. in broth culture by bioluminescence assay of atp.the luciferin-luciferase bioluminescence reaction was used to estimate cell numbers of campylobacter jejuni and campylobacter coli in broth cultures based on a linear relationship between cell numbers (in excess of 10(4) to 10(5] and atp levels. the sensitivity was lower than that obtained with escherichia coli. the calculated amount of intracellular atp per cell of c. jejuni and c. coli ranged from 1.7 to 2.1 fg.19853994377
[epidemiologic studies of enteritis caused by campylobacter jejuni. iv. a study of the principal reservoir hosts]. 19853995570
typing of heat-stable and heat-labile antigens of campylobacter jejuni and campylobacter coli by coagglutination.a coagglutination system has been devised for typing heat-stable and heat-labile antigens of campylobacter jejuni and c. coli. the use of protein a-positive staphylococcus aureus cells carrying campylobacter sp. serotype antibody and the treatment of campylobacter sp. cells with dnase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as smooth cultures. cells of s. aureus were sensitized with campylobacter sp. serotype antisera. four to fi ...19853998098
cadmium chloride susceptibility, a characteristic of campylobacter spp.we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ...19853998099
in vitro antimicrobial susceptibility, plasmid analysis, and serotyping of epidemic-associated campylobacter jejuni.campylobacter jejuni strains from 11 outbreaks were characterized by antimicrobial susceptibility, plasmid profile, and serotyping by the methods of lior et al. and penner and hennessy. all 31 strains were susceptible to erythromycin, clindamycin, chloramphenicol, kanamycin, tobramycin, streptomycin, and gentamicin. a total of 21 strains from nine outbreaks were resistant to one or more of the following antimicrobial agents: tetracycline, metronidazole, ampicillin, or carbenicillin. of the 31 st ...19853968203
campylobacter jejuni infections on college campuses: a national survey.we surveyed 303 colleges and universities to determine the frequency of isolations of campylobacter jejuni and other enteric bacterial pathogens. campylobacter cultures could be obtained at 74 per cent; 10 per cent of stool cultures yielded campylobacter, which was isolated 10 and 46 times more frequently than salmonella and shigella, respectively. the incidence of diagnosed campylobacter infections was 1.3 per 10,000 enrolled students per month. intercollegiate variation was not explained by di ...19854003634
campylobacter jejuni-specific serum antibodies are elevated in healthy bangladeshi children.in bangladesh and other developing countries, isolation of campylobacter jejuni is common in healthy children, and the illness/infection ratio falls with age. to determine whether specific serum antibodies correlate with this phenomenon, using an enzyme-linked immunosorbent assay, we studied sera from 93 healthy bangladeshi children and 121 healthy u.s. children under 15 years of age. for each age group (less than 1, 2 to 4, and 5 to 14 years) studied, specific serum antibody levels were signifi ...19853972984
the virulence of clinical and environmental isolates of campylobacter jejuni.the virulence of campylobacter jejuni and c. coli isolated from various water sources was compared with that of clinical strains by in vitro assays of adhesion, invasion and cytotoxicity to hela cells. variation in degree of attachment was observed, but this did not appear to be related to strain source, however, water strains were less invasive and less cytotoxic to hela cells than clinical strains as shown by immunofluorescence and electron microscopy. these differences were particularly evide ...19853973380
occurrence of campylobacter jejuni and giardia species in muskrat (ondatra zibethica).a total of 189 muskrat fecal samples were surveyed for campylobacter and giardia species. campylobacter jejuni was recovered from 47.5% of these samples, and giardia species were detected in 82.5%. these findings indicate that muskrat may be of importance to the health both of humans and of domestic animals.19853896141
pathogenic properties of campylobacter jejuni: assay and correlation with clinical manifestations.the pathogenic properties of 20 strains of campylobacter jejuni isolated from persons with clearly defined clinical manifestations were determined. cell-free broth filtrates were examined for (i) enterotoxin production by chinese hamster tissue culture assay and an enzyme-linked immunosorbent assay (elisa) employing gm1 ganglioside and affinity-purified antiserum to escherichia coli heat-labile toxin, (ii) cytotoxin production by vero and hela cell tissue culture lines, and (iii) their ability t ...19853899937
antigenicity of lipopolysaccharides from campylobacter jejuni and campylobacter coli in passive haemagglutination tests and enzyme-linked immunosorbent assays.passive haemagglutination tests and elisa were used to study the serological activity of homologous and heterologous rabbit antisera against lps prepared from various strains of c. jejuni/coli. in both test systems the homologous antisera exhibited serological activity against lps. the heterologous antisera showed some degree of intra- and inter- species cross-reactivity. the cross-reacting was most pronounced in the elisa. erythrocytes sensitized with untreated lps gave higher antibody-titres t ...19854013751
evaluation and diagnosis of acute infectious diarrhea.the appropriate approach to the diagnosis and management of acute infectious diarrhea is determined by the frequency and setting of the illness, the recognizable causes or syndromes, the cost and yield of available diagnostic tests, and the treatability of the disease. acute diarrhea affects everyone throughout the world from one to more than six times each year, depending on age, location, and living conditions. the range of identifiable viral, bacterial, and parasitic etiologies is great, and ...19854014291
comparison of four hippurate hydrolysis methods for identification of thermophilic campylobacter spp.the test for hippurate hydrolysis is critical for separation of campylobacter jejuni and c. coli strains. glycine and benzoic acid are formed when hippurate is hydrolyzed by c. jejuni. the test used in most laboratories is one of several variations of the ninhydrin tube test described by hwang and ederer (m. hwang and g. m. ederer, j. clin. microbiol. 1:114-115, 1975) for detection of glycine. we evaluated three modifications of the hwang and ederer method and the gas-liquid chromatographic (glc ...19853902875
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild animals.by using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ...19854015088
gastroenteritis due to campylobacter jejuni in lagos, nigeria. 19854016913
development of a bacteriophage typing system for campylobacter jejuni and campylobacter coli.a bacteriophage typing system for campylobacter jejuni and campylobacter coli was developed with phages isolated from poultry feces. data for phage selection were generated from a set of isolates of c. jejuni and c. coli from humans in illinois. selection of 14 phages from the 47 phages available was assisted by determination of the sneath-jaccard similarity coefficients and subsequent unweighted pair-group arithmetic averaging cluster analysis. the typing set was reproducible and stable in the ...19854019737
[campylobacter jejuni infections]. 19853908243
enrichment medium for isolation of campylobacter jejuni-campylobacter coli.a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ...19854026287
demonstration of antibodies reacting with escherichia coli heat-labile enterotoxin in sera of patients suffering from campylobacter jejuni/coli. 19853909022
immunological relationship of the b subunits of campylobacter jejuni and escherichia coli heat-labile enterotoxins.the application of dissociation techniques, involving gel filtration in the presence of guanidine, to a semipurified preparation of campylobacter jejuni heat-labile enterotoxin yielded a material whose functional and immunological properties resemble those of the b subunits of cholera toxin and escherichia coli heat-labile toxin (lt). the c. jejuni toxin b subunit reacted with gm1 ganglioside in an enzyme-linked immunosorbent assay, but lacked the holotoxin's cytotonic activity in the chinese ha ...19853922890
community-wide surveillance of campylobacter jejuni infection. evaluation of a laboratory-based method.surveillance of infections due to campylobacter jejuni is needed to further define the epidemiology of this disease in the u.s. we assessed one potential method of community-wide surveillance, a laboratory-based reporting network. six microbiology laboratories that routinely culture fecal specimens for c. jejuni reported all isolates to seattle-king county health department for an 18-month period. further investigations on 476 cases reported from this broadly based network revealed that c. jejun ...19854028667
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