Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| comparative genomics revealed multiple helicobacter pylori genes associated with biofilm formation in vitro. | biofilm formation by helicobacter pylori may be one of the factors influencing eradication outcome. however, genetic differences between good and poor biofilm forming strains have not been studied. | 2016 | 27870886 |
| a quantitative proteomic screen of the campylobacter jejuni flagellar-dependent secretome. | campylobacter jejuni is the leading cause of bacterial gastroenteritis in the world. a number of factors are believed to contribute to the ability of c. jejuni to cause disease within the human host including the secretion of non-flagellar proteins via the flagellar type iii secretion system (ft3ss). here for the first time we have utilised quantitative proteomics using stable isotope labelling by amino acids in cell culture (silac), and label-free liquid chromatography-mass spectrometry (lc/ms) ... | 2017 | 27865792 |
| epitope mapping of campylobacter jejuni flagellar capping protein (flid) by chicken (gallus gallus domesticus) sera. | campylobacter jejuni, a gram-negative rod, is a zoonotic pathogen associated with human acute bacterial gastroenteritis worldwide. the flagellum, composed of more than 35 proteins, is responsible for colonization of c. jejuni in the host gastrointestinal tract as well as inducing protective antibodies against the homologous serotype. in our previous study, we demonstrated that the flagellar capping protein (flid) is an immunodominant protein that reacted strongly to sera from field chickens. in ... | 2016 | 27865268 |
| structural diversity and biological importance of abo, h, lewis and secretor histo-blood group carbohydrates. | abo, h, secretor and lewis histo-blood system genes control the expression of part of the carbohydrate repertoire present in areas of the body occupied by microorganisms. these carbohydrates, besides having great structural diversity, act as potential receptors for pathogenic and non-pathogenic microorganisms influencing susceptibility and resistance to infection and illness. despite the knowledge of some structural variability of these carbohydrate antigens and their polymorphic levels of expre ... | 2016 | 27863762 |
| campylobacter-associated diseases in animals. | campylobacter includes a group of genetically diverse species causing a range of diseases in animals and humans. the bacterium is frequently associated with two economically important and epidemiologically distinct reproductive diseases in ruminants: enzootic infectious infertility in cattle owing to campylobacter fetus subsp. venerealis and abortions in sheep, goats, and cattle. septic abortion, usually epizootic in sheep, has been historically associated with c. fetus subsp. fetus and to a les ... | 2017 | 27860495 |
| isolation of campylobacter spp. from client-owned dogs and cats, and retail raw meat pet food in the manawatu, new zealand. | campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. the disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. the prevalence of campylobacter spp. in dogs and cats varies widely, and data on new zealand pets are limited. this study aimed to investigate the prevalence of campylobacter spp. in dogs, cats and retail raw meat pet food ... | 2016 | 27860343 |
| prevalence of enteropathogens in dogs attending 3 regional dog parks in northern california. | the prevalence and risk factors for infection with enteropathogens in dogs frequenting dog parks have been poorly documented, and infected dogs can pose a potential zoonotic risk for owners. | 2016 | 27859745 |
| antibiotics: practice and opinions of cambodian commercial farmers, animal feed retailers and veterinarians. | cambodia has reported multidrug resistant bacteria in poultry, similar to other countries in the region. we visited commercial food animal farms to explore opinions and antibiotic practices on the farms. | 2016 | 27857835 |
| application of probiotics for the production of safe and high-quality poultry meat. | poultry industry has always been a dynamic and integral part of national economies in many countries. economic losses incur especially in large-scale rearing facilities, often attributed to the deterioration of environmental conditions, poultry exposure to stressors and development of diseases. while antibiotics have been commonly used for prophylactic purposes and as growth stimulants, extensive documentation of antimicrobial resistance among pathogenic bacteria due to indiscriminate utilizatio ... | 2016 | 27857531 |
| guillain-barré syndrome: a century of progress. | in 1916, guillain, barré and strohl reported on two cases of acute flaccid paralysis with high cerebrospinal fluid protein levels and normal cell counts - novel findings that identified the disease we now know as guillain-barré syndrome (gbs). 100 years on, we have made great progress with the clinical and pathological characterization of gbs. early clinicopathological and animal studies indicated that gbs was an immune-mediated demyelinating disorder, and that severe gbs could result in seconda ... | 2016 | 27857121 |
| antimicrobial resistance of campylobacter species isolated from broilers in live bird markets in shanghai, china. | this study was conducted to determine the prevalence of antimicrobial resistance in campylobacter spp. isolates from broilers in live bird markets (lbms). a total of 209 campylobacter spp. isolates (84 campylobacter jejuni; 125 campylobacter coli) were recovered from 364 broiler cecum samples collected from five lbms in shanghai, china. minimum inhibitory concentrations of 13 antimicrobials were determined using agar dilution method. more than 96% of the campylobacter spp. isolates were resistan ... | 2017 | 27854542 |
| long-term impact after fulminant guillain-barré syndrome, case report and literature review. | a 47-year-old man was admitted to the intensive care unit a few hours after presenting to emergency department with acute diplopia and dysphonia. swallowing disorders and respiratory muscular weakness quickly required invasive ventilation. on day 3, the patient was in a "brain-death"-like state with deep coma and absent brainstem reflexes. electroencephalogram ruled out brain death diagnosis as a paradoxical sleep trace was recorded. cerebrospinal fluid analysis, electrophysiologic studies, and ... | 2016 | 27853394 |
| multiplex-touchdown pcr to simultaneously detect cryptosporidium parvum, giardia lamblia, and cyclospora cayetanensis, the major causes of traveler's diarrhea. | this study aimed to develop a multiplex-touchdown pcr method to simultaneously detect 3 species of protozoan parasites, i.e., cryptosporidium parvum, giardia lamblia, and cyclospora cayetanensis, the major causes of traveler's diarrhea and are resistant to standard antimicrobial treatments. the target genes included the cryptosporidium oocyst wall protein for c. parvum, glutamate dehydrogenase for g. lamblia, and 18s ribosomal rna (18s rrna) for c. cayetanensis. the sizes of the amplified fragme ... | 2016 | 27853120 |
| prevalence of campylobacter jejuni and campylobacter coli in raw milk and some dairy products. | this study was accomplished to test raw milk and certain dairy products sold in local markets of qena, egypt, for the presence of campylobacter coli and campylobacter jejuni. | 2016 | 27847427 |
| antibiotics in malaria therapy: which antibiotics except tetracyclines and macrolides may be used against malaria? | malaria, a parasite vector-borne disease, is one of the most significant health threats in tropical regions, despite the availability of individual chemoprophylaxis. malaria chemoprophylaxis and chemotherapy remain a major area of research, and new drug molecules are constantly being developed before drug-resistant parasites strains emerge. the use of anti-malarial drugs is challenged by contra-indications, the level of resistance of plasmodium falciparum in endemic areas, clinical tolerance and ... | 2016 | 27846898 |
| effects of seasonal meteorological variables on e. coli persistence in livestock faeces and implications for environmental and human health. | agriculture contributes significant volumes of livestock faeces to land. understanding how faecal microbes respond to shifts in meteorological patterns of contrasting seasons is important in order to gauge how environmental (and human health) risks may alter under a changing climate. the aim of this study was to: (i) quantify the temporal pattern of e. coli growth within dairy faeces post defecation; and (ii) derive e. coli seasonal population change profiles associated with contrasting environm ... | 2016 | 27845409 |
| differences in host breed and diet influence colonization by campylobacter jejuni and induction of local immune responses in chicken. | chickens are regarded as the main reservoir for human campylobacteriosis. little is known about the interaction between campylobacter jejuni (c. jejuni) and chickens. this interaction may be influenced by the stage of maturation of the immune system, developing gut microbiota composition and other factors including breed and diet. our aim was to investigate the impact of breed, and diet on c. jejuni colonization and host immune responses in chickens. birds were inoculated with 10(4) colony formi ... | 2016 | 27843492 |
| comparative genomic analysis of the flagellin glycosylation island of the gram-positive thermophile geobacillus. | protein glycosylation involves the post-translational attachment of sugar chains to target proteins and has been observed in all three domains of life. post-translational glycosylation of flagellin, the main structural protein of the flagellum, is a common characteristic among many gram-negative bacteria and archaea. several distinct functions have been ascribed to flagellin glycosylation, including stabilisation and maintenance of the flagellar filament, motility, surface recognition, adhesion, ... | 2016 | 27842516 |
| a novel phase variant of the cholera pathogen shows stress-adaptive cryptic transcriptomic signatures. | in a process known as phase variation, the marine bacterium and cholera pathogen vibrio cholerae alternately expresses smooth or rugose colonial phenotypes, the latter being associated with advanced biofilm architecture and greater resistance to ecological stress. to define phase variation at the transcriptomic level in pandemic v. cholerae o1 el tor strain n16961, we compared the rna-seq-derived transcriptomes among the smooth parent n16961, its rugose derivative (n16961r) and a smooth form obt ... | 2016 | 27842489 |
| subtyping of campylobacter jejuni ssp. doylei isolates using mass spectrometry-based phyloproteomics (mspp). | maldi-tof ms offers the possibility to differentiate some bacteria not only at the species and subspecies level but even below, at the strain level. allelic isoforms of the detectable biomarker ions result in isolate-specific mass shifts. mass spectrometry-based phyloproteomics (mspp) is a novel technique that combines the mass spectrometric detectable biomarker masses in a scheme that allows deduction of phyloproteomic relations from isolate specific mass shifts compared to a genome sequenced r ... | 2016 | 27842363 |
| development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese. | dairy products can harbor various microorganisms (e.g., campylobacter spp., salmonella spp., listeria monocytogenes, verocytotoxin-producing escherichia coli) arising from animal reservoirs, and which can become important sources of foodborne illness. therefore, early detection of food pathogens is crucial to prevent diseases. we wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddpcr) involving eight individual taqman™ reactions to detect ... | 2016 | 27840628 |
| 25 years of proton pump inhibitors: a comprehensive review. | proton pump inhibitors (ppis) were clinically introduced more than 25 years ago and have since proven to be invaluable, safe, and effective agents for the management of a variety of acid-related disorders. although all members in this class act in a similar fashion, inhibiting active parietal cell acid secretion, there are slight differences among ppis relating to their pharmacokinetic properties, metabolism, and food and drug administration (fda)-approved clinical indications. nevertheless, eac ... | 2016 | 27840364 |
| 25 years of proton pump inhibitors: a comprehensive review. | proton pump inhibitors (ppis) were clinically introduced more than 25 years ago and have since proven to be invaluable, safe, and effective agents for the management of a variety of acid-related disorders. although all members in this class act in a similar fashion, inhibiting active parietal cell acid secretion, there are slight differences among ppis relating to their pharmacokinetic properties, metabolism, and food and drug administration (fda)-approved clinical indications. nevertheless, eac ... | 2016 | 27840364 |
| compliance/non-compliance with biosecurity rules specified in the danish quality assurance system (kik) and campylobacter-positive broiler flocks 2012 and 2013. | one source for campylobacter jejuni infections in humans could be consumption of broiler meat. transmission of campylobacter into broiler houses/flocks occurs via many routes. a number of biosecurity rules is specified in the quality assurance system in danish chicken production (kik) - for which the broiler producers annually are audited for compliance with, by bureau veritas. multivariable logistic regression models were used to investigated the association between compliance/non-compliance wi ... | 2017 | 27838611 |
| complete genome sequences of group iii campylobacter bacteriophages pc5 and pc14. | here, we present the whole-genome sequences of bacteriophages pc5 and pc14 specific for campylobacter jejuni, a leading cause of gastroenteritis in developed countries. their genomes are syntenic to those of group iii campylobacter bacteriophages and share more than 90% identity at the nucleotide level with members of this group. | 2016 | 27834694 |
| small regulatory rnas of rickettsia conorii. | small regulatory rnas comprise critically important modulators of gene expression in bacteria, yet very little is known about their prevalence and functions in rickettsia species. r. conorii, the causative agent of mediterranean spotted fever, is a tick-borne pathogen that primarily infects microvascular endothelium in humans. we have determined the transcriptional landscape of r. conorii during infection of human microvascular endothelial cells (hmecs) by strand-specific rna sequencing to ident ... | 2016 | 27834404 |
| inhibitory effect of lactobacillus plantarum and lb. fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. | nosocomial infection constitutes a major public health problem worldwide. increasing antibiotic resistance of pathogens associated with nosocomial infections has also become a major therapeutic challenge for physicians. thus, development of alternative treatment protocols, such as the use of probiotics, matters. the aim of this research was to determine the antagonistic properties of lactobacillus plantarum and lb. fermentum isolated from the faeces of healthy infants against nonfermentative bac ... | 2016 | 27830081 |
| inhibitory effect of lactobacillus plantarum and lb. fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. | nosocomial infection constitutes a major public health problem worldwide. increasing antibiotic resistance of pathogens associated with nosocomial infections has also become a major therapeutic challenge for physicians. thus, development of alternative treatment protocols, such as the use of probiotics, matters. the aim of this research was to determine the antagonistic properties of lactobacillus plantarum and lb. fermentum isolated from the faeces of healthy infants against nonfermentative bac ... | 2016 | 27830081 |
| a new lab developed real time pcr assay for direct detection of c. difficle from stool sample without dna extraction. | clostridium difficile is a major cause of nosocomial antibiotic-associated infectious diarrhea and pseudomembranous colitis. detection of c. difficile by anaerobic bacterial culture and/or cytotoxicity assays has been largely replaced by rapid enzyme immunoassays (eia). however, due to the lack of sensitivity of stool eia, we developed a multiplex real-time pcr assay targeting the c. difficile toxin genes tcdb. stool samples from hospitalized pediatric patients suspected of having c. difficile-a ... | 2016 | 27829823 |
| in vitro susceptibility of four antimicrobials against riemerella anatipestifer isolates: a comparison of minimum inhibitory concentrations and mutant prevention concentrations for ceftiofur, cefquinome, florfenicol, and tilmicosin. | mutant prevention concentration (mpc) is an alternative pharmacodynamic parameter that has been used to measure antimicrobial activity and represents the propensities of antimicrobial agents to select resistant mutants. the concentration range between minimum inhibitory concentration (mic) and mpc is defined as mutant selection window (msw). the mpc and msw parameters represent the ability of antimicrobial agents to inhibit the bacterial mutants selected. this study was conducted to determine th ... | 2016 | 27829415 |
| aglh, a thermophilic udp-n-acetylglucosamine-1-phosphate:dolichyl phosphate glcnac-1-phosphotransferase initiating protein n-glycosylation pathway in sulfolobus acidocaldarius, is capable of complementing the eukaryal alg7. | aglh, a predicted udp-glcnac-1-phosphate:dolichyl phosphate glcnac-1-phosphotransferase, is initiating the protein n-glycosylation pathway in the thermoacidophilic crenarchaeon sulfolobus acidocaldarius. aglh successfully replaced the endogenous glcnac-1-phosphotransferase activity of alg7 in a conditional lethal saccharomyces cerevisiae strain, in which the first step of the eukaryal protein n-glycosylation process was repressed. this study is one of the few examples of cross-domain complementa ... | 2016 | 27822701 |
| aglh, a thermophilic udp-n-acetylglucosamine-1-phosphate:dolichyl phosphate glcnac-1-phosphotransferase initiating protein n-glycosylation pathway in sulfolobus acidocaldarius, is capable of complementing the eukaryal alg7. | aglh, a predicted udp-glcnac-1-phosphate:dolichyl phosphate glcnac-1-phosphotransferase, is initiating the protein n-glycosylation pathway in the thermoacidophilic crenarchaeon sulfolobus acidocaldarius. aglh successfully replaced the endogenous glcnac-1-phosphotransferase activity of alg7 in a conditional lethal saccharomyces cerevisiae strain, in which the first step of the eukaryal protein n-glycosylation process was repressed. this study is one of the few examples of cross-domain complementa ... | 2016 | 27822701 |
| pfge genotyping and molecular characterization of campylobacter spp. isolated from chicken meat. | a total of 70 samples were collected from chicken meat obtained from 10 markets in tehran, iran from which 39 campylobacter coli were isolated. among 10 antibiotics used, maximum resistance was seen to trimethoprim-sulphamethoxazole (sxt) (97.36%), nalidixic acid (94.8%), ciprofloxacin (87.7%), streptomycin (89.72%), and tetracycline (97.4%). no resistance was to gentamycin was observed. none of the campylobacter strains under study harbored integron, suggesting the involvement of other resistan ... | 2016 | 27822247 |
| a structural and functional study on the 2-c-methyl-d-erythritol-4-phosphate cytidyltransferase (ispd) from bacillus subtilis. | 2-c-methyl-d-erythritol-4-phosphate cytidyltransferase (ispd) is an essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis. this enzyme catalyzes 2-c-methyl-d-erythritol 4-phosphate (mep) and cytosine triphosphate (ctp) to 4-diphosphocytidyl-2-c-methyl-d-erythritol (cdpme) and inorganic pyrophosphate (ppi). bacillus subtilis was a kind of excellent isoprene producer. however, the studies on the key enzymes of mep pathway in b. subtilis were still absent. in this work, ... | 2016 | 27821871 |
| early electrodiagnostic features of upper extremity sensory nerves can differentiate axonal guillain-barré syndrome from acute inflammatory demyelinating polyneuropathy. | serial nerve conduction studies (ncss) are recommended for differentiating axonal and demyelinating guillain-barré syndrome (gbs), but this approach is not suitable for early diagnoses. this study was designed to identify possible ncs parameters for differentiating gbs subtypes. | 2016 | 27819421 |
| biomarkers of irritable bowel syndrome. | traditionally, irritable bowel syndrome (ibs) has not been regarded as an organic disease, and the pathophysiology of ibs is heterogeneous. currently, the diagnosis of ibs is based upon the rome diagnostic criteria. the performance of these criteria is only modest in predicting ibs, and moreover their validation is lacking. additionally, as functional symptoms are common in the general population, healthy controls or volunteers are difficult to define and there is currently no definition of "nor ... | 2017 | 27817184 |
| purification and characterization of a novel anti-campylobacter bacteriocin produced by lactobacillus curvatus dn317. | the lactic acid bacteria (lab) microbiota of saudi chicken ceca was determined. from 60 samples, 204 isolates of lactic acid bacteria were obtained. three isolates produced antimicrobial activities against campylobacter jejuni, listeria monocytogenes, and bacillus subtilis. the isolate dn317, which had the highest activity against campylobacter jejuni atcc 33560, was identified as lactobacillus curvatus (genbank accession numbers: kx353849 and kx353850). full inhibitory activity was observed aft ... | 2016 | 27812926 |
| evaluation and selection of bacillus species based on enzyme production, antimicrobial activity, and biofilm synthesis as direct-fed microbial candidates for poultry. | social concern about misuse of antibiotics as growth promoters (agp) and generation of multidrug-resistant bacteria have restricted the dietary inclusion of antibiotics in livestock feed in several countries. direct-fed microbials (dfm) are one of the multiple alternatives commonly evaluated as substitutes of agp. sporeformer bacteria from the genus bacillus have been extensively investigated because of their extraordinary properties to form highly resistant endospores, produce antimicrobial com ... | 2016 | 27812526 |
| complete structure of the bacterial flagellar hook reveals extensive set of stabilizing interactions. | the bacterial flagellar hook is a tubular helical structure made by the polymerization of multiple copies of a protein, flge. here we report the structure of the hook from campylobacter jejuni by cryo-electron microscopy at a resolution of 3.5 å. on the basis of this structure, we show that the hook is stabilized by intricate inter-molecular interactions between flge molecules. extra domains in flge, found only in campylobacter and in related bacteria, bring more stability and robustness to the ... | 2016 | 27811912 |
| microbial quality of reclaimed water for urban reuses: probabilistic risk-based investigation and recommendations. | although canada has abundant freshwater resources, many cities still experience seasonal water shortage. supply-side and demand-side management is a core strategy to address this water shortage. under this strategy, reclaimed water, which the canadian public is willing to use for non-potable purposes, is an option. however, no universal guidelines exist for reclaimed water use. despite the federal government's long-term goal to develop guidelines for many water reuse applications, guidelines hav ... | 2017 | 27810759 |
| mucosa-associated lymphoid tissue lymphoma of the sigmoid colon discovered on routine screening colonoscopy in patient with hepatitis c and helicobacter pylori infection. | mucosa-associated lymphoid tissue (malt) lymphoma is predominantly found in the stomach. rarely, it is found in the proximal colon and even less so in the sigmoid colon. we present a rare case of primary sigmoid colon malt lymphoma in a patient with concomitant helicobacter pylori and hepatitis c infection. we also review current imaging, staging, and therapeutic modalities. to our knowledge, this is the first sigmoid colon malt lymphoma reported in the united states. | 2016 | 27807552 |
| new weapons to fight old enemies: novel strategies for the (bio)control of bacterial biofilms in the food industry. | biofilms are microbial communities characterized by their adhesion to solid surfaces and the production of a matrix of exopolymeric substances, consisting of polysaccharides, proteins, dna and lipids, which surround the microorganisms lending structural integrity and a unique biochemical profile to the biofilm. biofilm formation enhances the ability of the producer/s to persist in a given environment. pathogenic and spoilage bacterial species capable of forming biofilms are a significant problem ... | 2016 | 27803696 |
| the risk of some veterinary antimicrobial agents on public health associated with antimicrobial resistance and their molecular basis. | the risk of antimicrobial agents used in food-producing animals on public health associated with antimicrobial resistance continues to be a current topic of discussion as related to animal and human public health. in the present review, resistance monitoring data, and risk assessment results of some important antimicrobial agents were cited to elucidate the possible association of antimicrobial use in food animals and antimicrobial resistance in humans. from the selected examples, it was apparen ... | 2016 | 27803693 |
| classification of a hypervirulent aeromonas hydrophila pathotype responsible for epidemic outbreaks in warm-water fishes. | lineages of hypervirulent aeromonas hydrophila (vah) are the cause of persistent outbreaks of motile aeromonas septicemia in warm-water fishes worldwide. over the last decade, this virulent lineage of a. hydrophila has resulted in annual losses of millions of tons of farmed carp and catfish in the people's republic of china and the united states (us). multiple lines of evidence indicate us catfish and asian carp isolates of a. hydrophila affiliated with sequence type 251 (st251) share a recent c ... | 2016 | 27803692 |
| occurrence of campylobacter spp. in poultry meat at retail and processing plants' levels in central italy. | human campylobacteriosis remains the most commonly reported gastrointestinal disease in europe and campylobacter (c.) jejuni and c. coli are the two species most frequently involved in such foodborne disease. based on the sampling plan established in the region of lazio (central italy) the aim of our work was to investigate the occurrence of campylobacter spp. in poultry meat preparations collected by the local veterinary authority at retail shops and processing plants. we also observed whether ... | 2016 | 27800429 |
| temporal variation of faecal shedding of escherichia coli 0157:h7 in a dairy herd producing raw milk for direct human consumption. | the objective of this study was to analyse over time the evolution of e. coli o157:h7 faecal shedding in a dairy herd producing raw milk for direct human consumption. the study was performed between october 2012 and september 2013 in an average size italian dairy farm where animals are housed inside the barn all over the year. the farm housed about 140 animals during the study - 70 cows and 70 calves and heifers. twenty-six animals were randomly selected from both the cows and young animals grou ... | 2014 | 27800362 |
| dynamic of campylobacter species contamination along a poultry slaughtering chain. | the prevalence of campylobacters was studied in a poultry farm and along the slaughtering chain. fifteen swabs from a farm and 75 samples (swabs and rinsates) from its slaughterhouse were collected. all the faecal and cloacal farm swabs were contaminated by campylobacter jejuni and c. coli against 50% for breast swabs. c. jejuni had a concentration of 6.26, 6.34 and 5.38 log10 cfu/ml in faecal, cloacal and breast swabs respectively. rinsates showed an almost constant concentration of campylobact ... | 2014 | 27800361 |
| occurrence of thermotolerant campylobacter in raw poultry meat, environmental and pigeon stools collected in open-air markets. | campylobacteriosis was the most commonly reported zoonosis for confirmed human cases in european union during 2011. poultry meat was very often implicated in campylobacter infections in humans. in italy commerce of raw poultry meat is common in open-air markets: these areas can be considered at high risk of bacterial contamination due to the high presence birds like pigeons. the aim of this study was to collect data about the contamination by thermotolerant campylobacter of raw poultry meat comm ... | 2014 | 27800355 |
| molecular epidemiology, virulence determinants and antimicrobial resistance of campylobacter spreading in retail chicken meat in central china. | campylobacter species are the major food-borne pathogens which could cause bacterial gastroenteritis in humans. contaminated chicken products have been recognized as the primary vehicles of campylobacter transmission to human beings. in this study, the prevalence of campylobacter in retail chicken meat in central china was investigated, and the isolates were further characterized using molecular approaches and tested for antibiotic resistance. | 2016 | 27800028 |
| travel-related illness at a tertiary care hospital in osaka, japan. | we analyzed the travel-related health problems in persons returning to japan from overseas. data were extracted retrospectively for all patients visiting the infectious diseases department of osaka city general hospital, osaka, japan, between july 2012 and september 2013. there were 209 sick returning travelers during the period of the study. the median age of the subjects was 34.3 years, and the subjects consisted of 133 tourists (63.6%), 39 business travelers (18.7%), 17 expatriates (8.1%), 14 ... | 2016 | 27799812 |
| a study of parasitic and bacterial pathogens associated with diarrhea in hiv-positive patients. | diarrhea is a common complication of acquired immune deficiency syndrome (aids), occurring in almost 90% of aids patients in developing countries like india. the present study was aimed to determine the prevalence and microbiological profile of pathogens associated with diarrhea in human immunodeficiency virus (hiv) positive patients and their relation to cd4 counts. | 2016 | 27795876 |
| high rates of contamination of poultry meat products with drug resistant campylobacter in metro manila, philippines. | a total of 265 chicken parts were collected from 15 wet markets and 15 supermarkets in metro manila, philippines. campylobacter spp. was isolated on modified charcoal cefoperazone deoxycholate agar plates and identified through biochemical tests and pcr amplification of genus and species specific genes. antimicrobial resistance profiles were determined following clinical and laboratory standards institute protocols. two hundred seven (78%) campylobacter spp. were isolated. c. jejuni and c. coli ... | 2016 | 27795478 |
| identification of residues in the lipopolysaccharide abc transporter that coordinate atpase activity with extractor function. | the surface of most gram-negative bacteria is covered with lipopolysaccharide (lps), creating a permeability barrier against toxic molecules, including many antimicrobials. to assemble lps on their surface, gram-negative bacteria must extract newly synthesized lps from the inner membrane, transport it across the aqueous periplasm, and translocate it across the outer membrane. the lpta to -g proteins assemble into a transenvelope complex that transports lps from the inner membrane to the cell sur ... | 2016 | 27795402 |
| clinical microbiology laboratories' adoption of culture-independent diagnostic tests is a threat to foodborne-disease surveillance in the united states. | introductionin november 2015, the centers for disease control and prevention (cdc) sent a letter to state and territorial epidemiologists, state and territorial public health laboratory directors, and state and territorial health officials. in this letter, culture-independent diagnostic tests (cidts) for detection of enteric pathogens were characterized as "a serious and current threat to public health surveillance, particularly for shiga toxin-producing escherichia coli (stec) and salmonella" t ... | 2017 | 27795338 |
| complete genome sequences of multidrug-resistant campylobacter jejuni strain 14980a (turkey feces) and campylobacter coli strain 14983a (housefly from a turkey farm), harboring a novel gentamicin resistance mobile element. | multidrug resistance (mdr) in foodborne pathogens is a major food safety and public health issue. here we describe whole-genome sequences of two mdr strains of campylobacter jejuni and campylobacter coli from turkey feces and a housefly from a turkey farm. both strains harbor a novel chromosomal gentamicin resistance mobile element. | 2016 | 27795285 |
| complete genomic sequence of campylobacter jejuni subsp. jejuni hs:19 strain rm1285 isolated from packaged chicken. | poultry products serve as the main source of campylobacter jejuni subsp. jejuni infections in humans. c. jejuni subsp. jejuni infections are a leading cause of foodborne gastroenteritis and are a prevalent antecedent to guillain-barré syndrome. this study describes the genome of c. jejuni subsp. jejuni hs:19 strain rm1285, isolated from packaged chicken in california. | 2016 | 27795263 |
| spyb, a small heme-binding protein, affects the composition of the cell wall in streptococcus pyogenes. | streptococcus pyogenes (group a streptococcus or gas) is a hemolytic human pathogen associated with a wide variety of infections ranging from minor skin and throat infections to life-threatening invasive diseases. the cell wall of gas consists of peptidoglycan sacculus decorated with a carbohydrate comprising a polyrhamnose backbone with immunodominant n-acetylglucosamine side-chains. all gas genomes contain the spyba operon, which encodes a 35-amino-acid membrane protein spyb, and a membrane-bo ... | 2016 | 27790410 |
| virulence and genomic feature of multidrug resistant campylobacter jejuni isolated from broiler chicken. | the aim of this study was to reveal the molecular mechanism involved in multidrug resistance and virulence of campylobacter jejuni isolated from broiler chickens. the virulence of six multidrug resistant c. jejuni was determined by in vitro and in vivo methods. the de novo whole genome sequencing technology and molecular biology methods were used to analyze the genomic features associated with the multidrug resistance and virulence of a selected isolate (c. jejuni 1655). the comparative genomic ... | 2016 | 27790202 |
| comparison of proteomics profiles of campylobacter jejuni strain bf under microaerobic and aerobic conditions. | campylobacter jejuni accounts for one of the leading causes of foodborne bacterial enteritis in humans. despite being considered an obligate microaerobic microorganism, c. jejuni is regularly exposed to oxidative stress. however, its adaptive strategies to survive the atmospheric oxygen level during transmission to humans remain unclear. recently, the clinical c. jejuni strain bf was singled out for its unexpected ability to grow under ambient atmosphere. here, we aimed to understand better the ... | 2016 | 27790195 |
| influence of haem environment on the catalytic properties of the tetrathionate reductase tsda from campylobacter jejuni. | bifunctional dihaem cytochrome c thiosulfate dehydrogenases/tetrathionate reductases (tsda) exhibit different catalytic properties depending on the source organism. in the human food-borne intestinal pathogen campylobacter jejuni, tsda functions as a tetrathionate reductase enabling respiration with tetrathionate as an alternative electron acceptor. in the present study, evidence is provided that cys(138) and met(255) serve as the sixth ligands of haem 1 and haem 2 respectively, in the oxidized ... | 2016 | 27789780 |
| genetic characteristics and multiple-pcr development for capsular identification of specific serotypes of campylobacter jejuni. | the polysaccharide capsule (cps) of campylobacter jejuni is a virulence factor linked to cell surface carbohydrate diversity which mainly determines the serotypes. thirty-four cps gene cluster structures have been published and some of them can be distinguished by multiple-pcr. penner serotypes hs1/44c, hs2, hs4c, hs19, hs23/36c and hs41 are markers for guillain-barré syndrome (gbs). the capsules may contribute to gbs susceptibility. analysis of 18 cps loci revealed high gene content diversity a ... | 2016 | 27788180 |
| acute hepatitis e in the us today occurs in diverse patient populations: case reports. | hepatitis e has long been thought of as an infection confined to the developing world. however, there has been an increased incidence of locally acquired cases in developed countries especially in transplant patients. our first case is a 56-year-old caucasian female post-heart transplant patient who presented with diarrhea and abdominal pain. she was found to be acutely infected with hepatitis e and progressed to stage 3 liver fibrosis. our second patient was an otherwise healthy 76-year-old vie ... | 2014 | 27785273 |
| fucosyllactose and l-fucose utilization of infant bifidobacterium longum and bifidobacterium kashiwanohense. | human milk oligosaccharides (hmos) are one of the major glycan source of the infant gut microbiota. the two species that predominate the infant bifidobacteria community, bifidobacterium longum subsp. infantis and bifidobacterium bifidum, possess an arsenal of enzymes including α-fucosidases, sialidases, and β-galactosidases to metabolise hmos. recently bifidobacteria were obtained from the stool of six month old kenyan infants including species such as bifidobacterium kashiwanohense, and bifidob ... | 2016 | 27782805 |
| campylobacteriosis in poland in 2013 and 2014 | evaluation of the epidemiological situation of campylobacteriosis in poland in 2013 and 2014 in comparison with the situation in the previous years. | 2017 | 27779836 |
| surface proteome mining for identification of potential vaccine candidates against campylobacter jejuni: an in silico approach. | campylobacter jejuni remains a major cause of human gastroenteritis with estimated annual incidence rate of 450 million infections worldwide. c. jejuni is a major burden to public health in both socioeconomically developing and industrialized nations. virulence determinants involved in c. jejuni pathogenesis are multifactorial in nature and not yet fully understood. despite the completion of the first c. jejuni genome project in 2000, there are currently no vaccines in the market against this pa ... | 2017 | 27778110 |
| inositol 1,4,5-trisphosphate receptor type 1 autoantibodies in paraneoplastic and non-paraneoplastic peripheral neuropathy. | recently, we described a novel autoantibody, anti-sj/itpr1-igg, that targets the inositol 1,4,5-trisphosphate receptor type 1 (itpr1) in patients with cerebellar ataxia. however, itpr1 is expressed not only by purkinje cells but also in the anterior horn of the spinal cord, in the substantia gelatinosa and in the motor, sensory (including the dorsal root ganglia) and autonomic peripheral nervous system, suggesting that the clinical spectrum associated with autoimmunity to itpr1 may be broader th ... | 2016 | 27776522 |
| cell resistance to the cytolethal distending toxin involves an association of dna repair mechanisms. | the cytolethal distending toxin (cdt), produced by many bacteria, has been associated with various diseases including cancer. cdt induces dna double-strand breaks (dsbs), leading to cell death or mutagenesis if misrepaired. at low doses of cdt, other dna lesions precede replication-dependent dsb formation, implying that non-dsb repair mechanisms may contribute to cdt cell resistance. to address this question, we developed a proliferation assay using human cell lines specifically depleted in each ... | 2016 | 27775089 |
| γ-glutamyltranspeptidase is an endogenous activator of toll-like receptor 4-mediated osteoclastogenesis. | chronic inflammation-associated bone destruction, which is observed in rheumatoid arthritis (ra) and periodontitis, is mediated by excessive osteoclastogenesis. we showed previously that γ-glutamyltranspeptidase (ggt), an enzyme involved in glutathione metabolism, acts as an endogenous activator of such pathological osteoclastogenesis, independent of its enzymatic activity. ggt accumulation is clinically observed in the joints of ra patients, and, in animals, the administration of recombinant gg ... | 2016 | 27775020 |
| sensitivity of direct culture, enrichment and pcr for detection of campylobacter jejuni and c. coli in broiler flocks at slaughter. | broiler chicken flocks are a significant source of campylobacter jejuni and campylobacter coli that result in the major public health problem of campylobacteriosis. accurate estimates of the prevalence of both c. coli and c. jejuni in flocks would enhance epidemiological understanding, risk assessment and control options. this study combined results from a panel of 10 detection tests (direct culture, enrichment and pcr) on caecal samples from flocks at slaughter. a parallel interpretation approa ... | 2016 | 27770505 |
| a comparative study of chip-seq sequencing library preparation methods. | chip-seq is the primary technique used to investigate genome-wide protein-dna interactions. as part of this procedure, immunoprecipitated dna must undergo "library preparation" to enable subsequent high-throughput sequencing. to facilitate the analysis of biopsy samples and rare cell populations, there has been a recent proliferation of methods allowing sequencing library preparation from low-input dna amounts. however, little information exists on the relative merits, performance, comparability ... | 2016 | 27769162 |
| antimicrobial-resistant campylobacter in organically and conventionally raised layer chickens. | poultry is a major source of campylobacter, which can cause foodborne bacterial gastroenteritis in humans. additionally, poultry-associated campylobacter can develop resistance to important antimicrobials, which increases the risk to public health. while broiler chickens have been the focus of many studies, the emergence of antimicrobial-resistant campylobacter on layer farms has not received equal attention. however, the growing popularity of cage-free and organic layer farming necessitates a c ... | 2017 | 27768387 |
| occurrence and genotypes of campylobacter species in broilers during the rearing period. | poultry are the main source of campylobacter infection worldwide. to obtain information on campylobacter-infected flocks and create a reference for preventing and controlling campylobacter at farm level, campylobacter isolates were recovered from broilers and the environments of nine chicken flocks in two farms during growth. the genetic relationship between the campylobacter isolates was determined using multilocus sequence typing. flocks were colonized as early as 3 weeks after introduction to ... | 2017 | 27766896 |
| a c-terminal coiled-coil region of cagl is responsible for helicobacter pylori-induced il-8 expression. | interleukin-8 (il-8) is a potent neutrophil-activating chemokine which triggers the infiltration and migration of neutrophils into areas of bacterial infection. helicobacter pylori-infected patient studies as well as animal models have revealed that h. pylori type i strains carrying an intact cytotoxin-associated gene pathogenicity island (cag-pai) with a functional type iv secretion system (t4ss) induce il-8 expression and secretion in gastric mucosa. this gastric mucosal il-8 expression correl ... | 2016 | 27766167 |
| association of campylobacter jejuni ssp. jejuni chemotaxis receptor genes with multilocus sequence types and source of isolation. | campylobacter jejuni's flagellar locomotion is controlled by eleven chemoreceptors. assessment of the distribution of the relevant chemoreceptor genes in the c. jejuni genomes deposited in the national center for biotechnology information (ncbi) database led to the identification of two previously unknown tlp genes and a tlp5 pseudogene. these two chemoreceptor genes share the same locus in the c. jejuni genome with tlp4 and tlp11, but the gene region encoding the periplasmic ligand binding doma ... | 2016 | 27766165 |
| a direct-sensing galactose chemoreceptor recently evolved in invasive strains of campylobacter jejuni. | a rare chemotaxis receptor, tlp11, has been previously identified in invasive strains of campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that tlp11 specifically interacts with galactose. tlp11 is required for the chemotactic response of c. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. the inactivated mutant displays reduced ... | 2016 | 27762269 |
| clinical heterogeneity of guillain-barré syndrome in the emergency department: impact on clinical outcome. | guillain-barré syndrome (gbs) is mainly classified into acute inflammatory demyelinating polyneuropathy (aidp) and acute motor axonal neuropathy (aman). although diagnosis of gbs requires progressive weakness and universal areflexia or hyporeflexia, cases of gbs with preserved or increased deep tendon reflexes (dtrs) have been increasingly recognized. we report three cases of gbs, presenting at a single unit in six months. our first case presented with pure sensory symptoms. the second case had ... | 2016 | 27761272 |
| chicken immune response after in ovo immunization with chimeric tlr5 activating flagellin of campylobacter jejuni. | campylobacter jejuni is the main cause of bacterial food-borne diseases in developed countries. chickens are the most important source of human infection. vaccination of poultry is an attractive strategy to reduce the number of c. jejuni in the intestinal tract of chickens. we investigated the immunogenicity and protective efficacy of a recombinant c. jejuni flagellin-based subunit vaccine with intrinsic adjuvant activity. toll-like receptor activation assays demonstrated the purity and tlr5 sti ... | 2016 | 27760175 |
| variations of phosphorous accessibility causing changes in microbiome functions in the gastrointestinal tract of chickens. | the chicken gastrointestinal tract (git) harbours a complex microbial community, involved in several physiological processes such as host immunomodulation and feed digestion. for the first time, the present study analysed dietary effects on the protein inventory of the microbiome in crop and ceca of broilers. we performed quantitative label-free metaproteomics by using 1-d-gel electrophoresis coupled with lc-ms/ms to identify the structural and functional changes triggered by diets supplied with ... | 2016 | 27760159 |
| structural insights into bacterial flagellar hooks similarities and specificities. | across bacteria, the protein that makes the flagellar hook, flge, has a high variability in amino acid residue composition and sequence length. we hereby present the structure of two fragments of flge protein from campylobacter jejuni and from caulobacter crescentus, which were obtained by x-ray crystallography, and a high-resolution model of the hook from caulobacter. by comparing these new structures of flge proteins, we show that bacterial hook can be divided in two distinct parts. the first ... | 2016 | 27759043 |
| the type b flagellin of hypervirulent clostridium difficile is modified with novel sulfonated peptidylamido-glycans. | glycosylation of flagellins is a well recognized property of many bacterial species. in this study, we describe the structural characterization of novel flagellar glycans from a number of hypervirulent strains of c. difficile we used mass spectrometry (nano-lc-ms and ms/ms analysis) to identify a number of putative glycopeptides that carried a variety of glycoform substitutions, each of which was linked through an initial n-acetylhexosamine residue to ser or thr. detailed analysis of a lldgsstei ... | 2016 | 27758867 |
| fitness cost of fluoroquinolone resistance in clinical isolates of pseudomonas aeruginosa differs by type iii secretion genotype. | fluoroquinolone (fq) resistance is highly prevalent among clinical strains of pseudomonas aeruginosa, limiting treatment options. we have reported previously that highly virulent strains containing the exou gene of the type iii secretion system are more likely to be fq-resistant than strains containing the exos gene, as well as more likely to acquire resistance-conferring mutations in gyra/b and parc/e. we hypothesize that fq-resistance imposes a lower fitness cost on exou compared to exos strai ... | 2016 | 27757111 |
| use of plant extracts as an effective manner to control clostridium perfringens induced necrotic enteritis in poultry. | necrotic enteritis (ne) is an important concern in poultry industry since it causes economic losses, increased mortality, reduction of bird welfare, and contamination of chicken products for human consumption. for decades, the use of in-feed antimicrobial growth promoters (agps) has been the main strategy to control intestinal pathogens including clostridium perfringens (cp), the causative agent of ne. however, the use of agps in animal diet has been linked to the emergence and transmission of a ... | 2016 | 27747227 |
| use of direct lamp screening of broiler fecal samples for campylobacter jejuni and campylobacter coli in the positive flock identification strategy. | rapid identification of campylobacter-positive flocks before slaughter, following freezing and heat treatment for the campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. we evaluated a loop-mediated isothermal amplification (lamp) assay for the direct screening of naturally contaminated chicken cloacal swabs for c. jejuni/c. coli to compare this assay with conventional quantitative culture methods. in a comparison study o ... | 2016 | 27746777 |
| unique and universal features of epsilonproteobacterial origins of chromosome replication and dnaa-dnaa box interactions. | in bacteria, chromosome replication is initiated by the interaction of the initiator protein dnaa with a defined region of a chromosome at which dna replication starts (oric). while dnaa proteins share significant homology regardless of phylogeny, oric regions exhibit more variable structures. the general architecture of orics is universal, i.e., they are composed of a cluster of dnaa binding sites, a dna-unwinding element, and sequences that bind regulatory proteins. however, detailed structure ... | 2016 | 27746772 |
| endophytes: a treasure house of bioactive compounds of medicinal importance. | endophytes are an endosymbiotic group of microorganisms that colonize in plants and microbes that can be readily isolated from any microbial or plant growth medium. they act as reservoirs of novel bioactive secondary metabolites, such as alkaloids, phenolic acids, quinones, steroids, saponins, tannins, and terpenoids that serve as a potential candidate for antimicrobial, anti-insect, anticancer and many more properties. while plant sources are being extensively explored for new chemical entities ... | 2016 | 27746767 |
| epidemiological and clinical profile of adult patients with blastocystis sp. infection in barcelona, spain. | blastocystis spp. are among the most frequently observed intestinal parasites in humans. despite the discovery of blastocystis approximately 100 years ago, limited information is available regarding its pathogenesis, genetic diversity, and available treatment options. the aim of this study was to describe the epidemiological and clinical characteristics of patients with blastocystis sp. infections diagnosed at vall d'hebron university hospital (barcelona, spain). | 2016 | 27741951 |
| primary b-cell mucosa-associated lymphoid tissue lymphoma of the hard palate and parotid gland: report of one case and review of the literature. | a 61-year-old woman was admitted to our hospital with an ulcerated palate mass and swelling of the right parotid gland. incisional biopsy from the hard palate revealed an extranodal marginal zone b-cell lymphoma, also called mucosa-associated lymphoid tissue (malt) lymphoma. final diagnosis was malt lymphoma of the parotid gland with concomitant involvement of an extremely seldom site of involvement: the hard palate. to our knowledge, this report illustrates the first case of malt lymphoma of th ... | 2016 | 27738485 |
| genomic comparison of campylobacter spp. and their potential for zoonotic transmission between birds, primates, and livestock. | campylobacter is the leading cause of human gastroenteritis worldwide. wild birds, including american crows, are abundant in urban, suburban, and agricultural settings and are likely zoonotic vectors of campylobacter their proximity to humans and livestock increases the potential spreading of campylobacter via crows between the environment, livestock, and humans. however, no studies have definitively demonstrated that crows are a vector for pathogenic campylobacter we used genomics to evaluate t ... | 2016 | 27736787 |
| bacterial intra-species gene loss occurs in a largely clocklike manner mostly within a pool of less conserved and constrained genes. | gene loss is a major contributor to the evolution of bacterial gene content. gene loss may occur as a result of shifts in environment leading to changes in the intensity and/or directionality of selection applied for the maintenance of specific genes. gene loss may also occur in a more neutral manner, when gene functions are lost that were not subject to strong selection to be maintained, irrespective of changes to environment. here, we used a pangenome-based approach to investigate patterns of ... | 2016 | 27734920 |
| exploiting bacterial whole-genome sequencing data for evaluation of diagnostic assays: campylobacter species identification as a case study. | the application of whole-genome sequencing (wgs) to problems in clinical microbiology has had a major impact on the field. clinical laboratories are now using wgs for pathogen identification, antimicrobial susceptibility testing, and epidemiological typing. wgs data also represent a valuable resource for the development and evaluation of molecular diagnostic assays, which continue to play an important role in clinical microbiology. to demonstrate this application of wgs, this study used publicly ... | 2016 | 27733632 |
| spatial and temporal shifts in bacterial biogeography and gland occupation during the development of a chronic infection. | gland colonization may be one crucial route for bacteria to maintain chronic gastrointestinal infection. we developed a quantitative gland isolation method to allow robust bacterial population analysis and applied it to the gastric pathobiont helicobacter pylori after infections in the murine model system, h. pylori populations multiply both inside and outside glands in a manner that requires the bacteria to be motile and chemotactic. h. pylori is able to achieve gland densities averaging 25 to ... | 2016 | 27729513 |
| survival and evolution of crispr-cas system in prokaryotes and its applications. | prokaryotes have developed numerous innate immune mechanisms in order to fend off bacteriophage or plasmid attack. one of these immune systems is clustered regularly interspaced short palindromic repeats (crispr). crispr-associated proteins play a key role in survival of prokaryotes against invaders, as these systems cleave dna of foreign genetic elements. beyond providing immunity, these systems have significant impact in altering the bacterial physiology in term of its virulence and pathogenic ... | 2016 | 27725818 |
| antimicrobial resistance of salmonella serovars and campylobacter spp. isolated from an opportunistic gull species, yellow-legged gull ( larus michahellis ). | wildlife is a natural reservoir of salmonella and campylobacter, the most important human foodborne pathogens worldwide. free-living birds have the potential to transport, over large distances, such zoonotic bacteria that may harbor antimicrobial resistance traits. on the northeastern iberian coast, we assessed the role of yellow-legged gulls ( larus michahellis ) as reservoirs of antimicrobial resistance in salmonella and thermophilic campylobacter isolates recovered from gulls at three colonie ... | 2017 | 27723383 |
| gastric adenocarcinoma in a patient with x-linked agammaglobulinemia and hiv: case report and review of the literature. | 2016 | 27722150 | |
| identification of large disjoint motifs in biological networks. | biological networks provide great potential to understand how cells function. network motifs, frequent topological patterns, are key structures through which biological networks operate. finding motifs in biological networks remains to be computationally challenging task as the size of the motif and the underlying network grow. often, different copies of a given motif topology in a network share nodes or edges. counting such overlapping copies introduces significant problems in motif identificat ... | 2016 | 27716036 |
| the gut microbiome as therapeutic target in central nervous system diseases: implications for stroke. | research on commensal microbiota and its contribution to health and disease is a new and very dynamically developing field of biology and medicine. recent experimental and clinical investigations underscore the importance of gut microbiota in the pathogenesis and course of stroke. importantly, microbiota may influence the outcome of cerebral ischemia by modulating central nervous system antigen-specific immune responses. in this review we summarize studies linking gut microbiota with physiologic ... | 2016 | 27714645 |
| cell-free spent media obtained from bifidobacterium bifidum and bifidobacterium crudilactis grown in media supplemented with 3'-sialyllactose modulate virulence gene expression in escherichia coli o157:h7 and salmonella typhimurium. | complex oligosaccharides from human milk (hmo) possess an antimicrobial activity and can promote the growth of bifidobacteria such as bifidobacterium bifidum and bifidobacterium longum subsp. infantis. in addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. whey is rich in complex bovine milk oligosaccharides (bmo) structurally similar to hmo and b. crudilactis, a species of bovine ... | 2016 | 27713728 |
| the bacterial mfd protein prevents dna damage induced by the host nitrogen immune response in a ner-independent but recbc-dependent pathway. | production of reactive nitrogen species is an important component of the host immune defence against bacteria. here, we show that the bacterial protein mfd (mutation frequency decline), a highly conserved and ubiquitous bacterial protein involved in dna repair, confers bacterial resistance to the eukaryotic nitrogen response produced by macrophage cells and during mice infection. in addition, we show that recbc is also necessary to survive this stress. the inactivation of recbc and mfd genes is ... | 2016 | 27711223 |
| identification of ompa-like protein of tannerella forsythia as an o-linked glycoprotein and its binding capability to lectins. | bacterial glycoproteins are associated with physiological and pathogenic functions of bacteria. it remains unclear whether bacterial glycoproteins can bind to specific classes of lectins expressed on host cells. tannerella forsythia is a gram-negative oral anaerobe that contributes to the development of periodontitis. in this study, we aimed to find lectin-binding glycoproteins in t. forsythia. we performed affinity chromatography of wheat germ agglutinin, which binds to n-acetylglucosamine (glc ... | 2016 | 27711121 |
| anti-campylobacter activity of resveratrol and an extract from waste pinot noir grape skins and seeds, and resistance of camp. jejuni planktonic and biofilm cells, mediated via the cmeabc efflux pump. | to define anti-campylobacter jejuni activity of an extract from waste skins and seeds of pinot noir grapes (gss), resveratrol and possible resistance mechanisms, and the influence of these on camp. jejuni morphology. | 2017 | 27709726 |