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isolation and characterization of a non-specific lipid transfer protein from chelidonium majus l. latex.plant non-specific lipid transfer proteins (nsltps) are small basic proteins, which mostly play a role in intracellular lipid transport and antimicrobial defense. recently it was shown using shotgun proteomic approach that the whole plant extract of chelidonium majus l. (papaveraceae) contains relatively abundant nsltps. therefore the aim of the work was to isolate and characterize nsltp from c. majus latex. results obtained using pcr approach with degenerate primers showed the presence of nsltp ...201728619636
mlst genotypes of campylobacter jejuni isolated from broiler products, dairy cattle and human campylobacteriosis cases in lithuania.campylobacter (c.) jejuni is the leading cause of human campylobacteriosis worldwide. we performed a molecular epidemiological study to investigate the genetic relationship among c. jejuni strains isolated from human diarrhoeal patients, broiler products and dairy cattle in lithuania.201728619013
differential survival of hyper-aerotolerant campylobacter jejuni under different gas conditions.campylobacter jejuni accounts for a significant number of foodborne illnesses around the world. c. jejuni is microaerophilic and typically does not survive efficiently in oxygen-rich conditions. we recently reported that hyper-aerotolerant (hat) c. jejuni are highly prevalent in retail poultry meat. to assess the capabilities of hat c. jejuni in foodborne transmission and infection, in this study, we investigated the prevalence of virulence genes in hat c. jejuni and the survival in poultry meat ...201728611753
rnaseq reveals complex response of campylobacter jejuni to ovine bile and in vivo gallbladder environment.colonization of the gallbladder by enteric pathogens such as salmonella typhi, listeria monocytogenes, and campylobacter jejuni is thought to play a key role in transmission and persistence of these important zoonotic agents; however, little is known about the molecular mechanisms that allow for bacterial survival within this harsh environment. recently, a highly virulent c. jejuni sheep abortion (sa) clone represented by the clinical isolate ia3902 has emerged as the dominant cause for sheep ab ...201728611744
campylobacter jejuni bacteremia in a liver cirrhosis patient and review of literature: a case study.campylobacter infection causes gastrointestinal symptoms such as abdominal pain or diarrhea. occasionally, campylobacter bacteremia affects immunocompromised patients; however, serious outcomes are known to be rare. here, we present a case of a patient with campylobacter bacteremia who had underlying liver cirrhosis. the patient had fever and diarrhea. these symptoms subsided after treatment with cefotaxime. campylobacter jejuni was isolated in the blood culture after 10 days. in addition, previ ...201728608661
phage exposure causes dynamic shifts in the expression states of specific phase-variable genes of campylobacter jejuni.phase variation (pv) creates phenotypic heterogeneity at high frequencies and in a reversible manner. this phenomenon allows bacteria to adapt to a variety of different environments and selective pressures. in campylobacterjejuni this reversible adaptive process is mediated by mutations in homopolymeric g/c tracts. many c. jejuni-specific phages are dependent on phase-variable surface structures for successful infection. we previously identified the capsular polysaccharide (cps) moiety, meopn-ga ...201728597819
towards diagnostic metagenomics of campylobacter in fecal samples.the development of diagnostic metagenomics is driven by the need for universal, culture-independent methods for detection and characterization of pathogens to substitute the time-consuming, organism-specific, and often culture-based laboratory procedures for epidemiological source-tracing. some of the challenges in diagnostic metagenomics are, that it requires a great next-generation sequencing depth and unautomated data analysis.201728595575
immune responses upon campylobacter jejuni infection of secondary abiotic mice lacking nucleotide-oligomerization-domain-2.campylobacter jejuni infections are of rising importance worldwide. given that innate immune receptors including nucleotide-oligomerization-domain-2 (nod2) are essentially involved in combating enteropathogenic infections, we here surveyed the impact of nod2 in murine campylobacteriosis.201728592996
generation of the membrane potential and its impact on the motility, atp production and growth in campylobacter jejuni.the generation of a membrane potential (δψ), the major constituent of the proton motive force (pmf), is crucial for atp synthesis, transport of nutrients and flagellar rotation. campylobacter jejuni harbors a branched electron transport chain, enabling respiration with different electron donors and acceptors. here, we demonstrate that a relatively high δψ is only generated in the presence of either formate as electron donor or oxygen as electron acceptor, in combination with an acceptor/donor re ...201728586527
confirmed identification and toxin profiling of campylobacter jejuni using a thermostabilized multiplex pcr formulation.cytolethal distending toxin (cdt) producing campylobacter jejuni species are one of the leading causes of human gastroenteritis worldwide. the main intent of the study was to develop a multiplex pcr assay for the confirmed identification and toxin profiling of c. jejuni. the genes targeted were rpo b as genus specific, hip o for species; cdt a, cdt b, cdt c encoding respective subunit proteins of cdt with internal amplification control (iac). to enhance its application as a pre-mixed ready-to-us ...201728574617
pathogen-induced ubiquitin-editing enzyme a20 bifunctionally shuts off nf-κb and caspase-8-dependent apoptotic cell death.the human pathogen helicobacter pylori infects more than half of the world's population and is a paradigm for persistent yet asymptomatic infection but increases the risk for chronic gastritis and gastric adenocarcinoma. for successful colonization, h. pylori needs to subvert the host cell death response, which serves to confine pathogen infection by killing infected cells and preventing malignant transformation. infection of gastric epithelial cells by h. pylori provokes direct and fast activat ...201728574503
profiling of campylobacter jejuni proteome in exponential and stationary phase of growth.campylobacter jejuni has been reported as a major cause of bacterial food-borne enteritides in developed countries during the last decade. despite its fastidious growth requirements, including low level of oxygen and high level of co2, this pathogen is able to persist in the environment without permanent loss of its viability and virulence. as c. jejuni is not able to multiply outside a host, the cells spend significant amount of time in stationary phase of growth. the entry into the stationary ...201728572800
a case of myopericarditis associated to campylobacter jejuni infection in the southern hemisphere.myopericarditis is an infrequent complication of acute diarrheal illness due to campylobacter jejuni, and it has been mainly reported in developed nations. the first case detected in chile--an upper-middle income country--that is coincidental with the increasing importance of acute gastroenteritis associated to this pathogen, is described. recognition of this agent in stools requires special laboratory techniques not widely available, and it was suspected when a young patient presented with acut ...201722729200
development of an isothermal amplification-based assay for the rapid visual detection of salmonella bacteria.the efficient and timely detection of pathogens is a major concern worldwide. the aim of this study was to establish a rapid detection method for salmonella bacteria in food samples to facilitate timely treatment. widely used detection methods currently include culture-based methods and pcr-based methods. the former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. in this study, a sensiti ...201728711269
a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay.salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ...201728526589
development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens.bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ...201728505584
next generation sequencing-based multigene panel for high throughput detection of food-borne pathogens.contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. bacteria that cause foodborne diseases are known as foodborne pathogens (fbps). accurate detection methods that are able to reveal the presence of fbps in food matrices are in constant demand, in order to ensure safe foods wi ...201728578266
generation of recombinant n-linked glycoproteins in e. coli.the production of n-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, escherichia coli. this methods chapter will outline the components and procedures needed to produce n-linked glycoproteins in e. coli, utilizing campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. to ensure a successful outcome, various methods will be highlighted that c ...201728470609
isolation of campylobacter species from stool samples by use of a filtration method: assessment from a united states-based population.fecal samples submitted to our clinical microbiology laboratory from patients in the philadelphia region were prospectively analyzed for campylobacter species other than c. jejuni and c. coli using a filtration method and microaerobic conditions with increased h2 concentrations. of 225 samples tested, 13 (5.8%) yielded campylobacter species, with frequent isolation of c. concisus the majority of campylobacter species were not clinically significant. additional studies in u.s. populations are war ...201728468859
phase-variable changes in the position of o-methyl phosphoramidate modifications on the polysaccharide capsule of campylobacter jejuni modulate serum resistance.campylobacter jejuni polysaccharide capsules (cps) are characterized by the presence of nonstoichiometric o-methyl phosphoramidate (meopn) modifications. the lack of stoichiometry is due to phase variation at homopolymeric tracts within the meopn transferase genes. c. jejuni strain 81-176 contains two meopn transferase genes and has been shown previously to contain meopn modifications at the 2 and 6 positions of the galactose (gal) moiety in the cps. we demonstrate here that one of the two meopn ...201728461446
genome-wide fitness analyses of the foodborne pathogen campylobacter jejuni in in vitro and in vivo models.campylobacter is the most common cause of foodborne bacterial illness worldwide. faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. there is a lack of insight into the mechanisms driving c. jejuni growth and survival within hosts and the environment. here, we report a detailed analysis of c. jejuni fitness across models reflecting stages in its life cycle. transposon (tn) gene-inactivation libraries were generated in three c. jej ...201728455506
core genome multilocus sequence typing scheme for stable, comparative analyses of campylobacter jejuni and c. coli human disease isolates.human campylobacteriosis, caused by campylobacter jejuni and c. coli, remains a leading cause of bacterial gastroenteritis in many countries, but the epidemiology of campylobacteriosis outbreaks remains poorly defined, largely due to limitations in the resolution and comparability of isolate characterization methods. whole-genome sequencing (wgs) data enable the improvement of sequence-based typing approaches, such as multilocus sequence typing (mlst), by substantially increasing the number of l ...201728446571
nonspecific effects of oral polio vaccine on diarrheal burden and etiology among bangladeshi infants.as the global polio eradication initiative prepares to cease use of oral polio vaccine (opv) in 2020, there is increasing interest in understanding if oral vaccination provides non-specific immunity to other infections so that the consequences of this transition can be effectively planned for and mitigated.201728444240
correlation between gyra and cmer box polymorphism and fluoroquinolone resistance in campylobacter jejuni isolates in china.sequence analysis of 79 ciprofloxacin-resistant campylobacter jejuni isolates collected in china showed resistance-related sequence variations in gyra and cmer-box. all the isolates contain an identical thr-86-ile substitution in gyra. several novel cmer-box variations, including point substitutions, deletion, and insertion, were identified. the point insertion or deletion led to dramatically reduced binding of cmer to the cmeabc promoter, which significantly increases the expression of cmeabc a ...201728438942
multiple pathogen biomarker detection using an encoded bead array in droplet pcr.we present a droplet pcr workflow for detection of multiple pathogen dna biomarkers using fluorescent color-coded luminex® beads. this strategy enables encoding of multiple singleplex droplet pcrs using a commercially available bead set of several hundred distinguishable fluorescence codes. this workflow provides scalability beyond the limited number offered by fluorescent detection probes such as taqman probes, commonly used in current multiplex droplet pcrs. the workflow was validated for thre ...201728434824
[campylobacter jejuni o:19 serotype in argentine poultry meat supply chain].thermotolerant species of campylobacter have been focus of attention in the last years because they are the major agent causing zoonotic foodborne diseases. in addition, campylobacter jejuni o:19 serotype was associated with guillain barré syndrome. the aim of this study was to determine the proportion of c. jejuni o:19 serotype isolated at different stages of three poultry meat supply chain in santa fe, argentina. the analysis showed that 18% of isolated c. jejuni belong to serotype o:19. it wa ...201728431787
application of β-resorcylic acid as potential antimicrobial feed additive to reduce campylobacter colonization in broiler chickens.campylobacter is one of the major foodborne pathogens that result in severe gastroenteritis in humans, primarily through consumption of contaminated poultry products. chickens are the reservoir host of campylobacter, where the pathogen colonizes the ceca, thereby leading to contamination of carcass during slaughter. a reduction in cecal colonization by campylobacter would directly translate into reduced product contamination and risk of human infections. with increasing consumer demand for antib ...201728428779
newer insights into the antidiarrheal effects of acacia catechu willd. extract in guinea pig.acacia catechu willd. is a plant diffused in india and other asian countries, where it is used as a traditional medicine for the treatment of several ailments including diarrhea, one of the most common pathologies worldwide. in this study, we determined the chemical composition of acacia catechu willd. extract (ac) and evaluated its effect on spontaneous and induced contractility in isolated guinea pig ileum and proximal colon. preliminary data about its antimicrobial effect against some pathoge ...201728422543
draft genome sequence of multidrug-resistant abortive campylobacter jejuni from northern california.campylobacter jejuni is an enteric bacterium that can cause abortion in livestock. this is the release of a multidrug-resistant campylobacter jejuni genome from an isolate that caused an abortion in a cow in northern california. this isolate is part of the 100k pathogen genome project.201728408683
accessory genetic content in campylobacter jejuni st21cc isolates from feces and blood.campylobacter jejuni is an important foodborne pathogen and the most commonly reported bacterial cause of gastroenteritis. c. jejuni is occasionally found in blood, although mechanisms important for invasiveness have remained unclear. c. jejuni is divided into many different lineages, of which the st21 clonal complex (cc) is widely distributed. here, we performed comparative genomic and in vitro analyses on 17c. jejuni st21cc strains derived from human blood and feces in order to identify featur ...201728408091
rv3634c from mycobacterium tuberculosis h37rv encodes an enzyme with udp-gal/glc and udp-galnac 4-epimerase activities.a bioinformatics study revealed that mycobacterium tuberculosis h37rv (mtb) contains sequence homologs of campylobacter jejuni protein glycosylation enzymes. the orf rv3634c from mtb was identified as a sequence homolog of c. jejuni udp-gal/galnac 4-epimerase. this study reports the cloning of rv3634c and its expression as an n-terminal his-tagged protein. the recombinant protein was shown to have udp-gal/glc 4-epimerase activity by god-pod assay and by reverse phase hplc. this enzyme was shown ...201728403215
the periplasmic chaperone network of campylobacter jejuni: evidence that salc (cj1289) and ppid (cj0694) are involved in maintaining outer membrane integrity.the outer membrane (om) of gram-negative pathogenic bacteria is a key structure in host-pathogen interactions that contains a plethora of proteins, performing a range of functions including adhesion, nutrient uptake, export of effectors and interaction with innate and adaptive components of the immune system. in addition, the om can exclude drugs and thus contribute to antimicrobial resistance. the om of the food-borne pathogen campylobacter jejuni contains porins, adhesins and other virulence f ...201728400767
campylobacter jejuni survival in a poultry processing plant environment.campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. consumption of poultry, especially chicken's meat is considered the most common route for human infection. the aim of this study was to determine if campylobacter spp. might persist in the poultry plant environment before and after cleaning and disinfection procedures and the distribution and their genetic relatedness. during one month from a poultry plant were analyzed a total of 494 samples -defeathering machin ...201728400001
sourcer: classification and source attribution of infectious agents among heterogeneous populations.zoonotic diseases are a major cause of morbidity, and productivity losses in both human and animal populations. identifying the source of food-borne zoonoses (e.g. an animal reservoir or food product) is crucial for the identification and prioritisation of food safety interventions. for many zoonotic diseases it is difficult to attribute human cases to sources of infection because there is little epidemiological information on the cases. however, microbial strain typing allows zoonotic pathogens ...201728558033
whole-genome sequence of the bacteriophage-sensitive strain campylobacter jejuni nctc12662.campylobacter jejuni nctc12662 has been the choice bacteriophage isolation strain due to its susceptibility to c. jejuni bacteriophages. this trait makes it a good candidate for studying bacteriophage-host interactions. we report here the whole-genome sequence of nctc12662, allowing future elucidation of the molecular mechanisms of phage-host interactions in c. jejuni.201728546493
metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen campylobacter jejuni.campylobacter jejuni is one of the leading infectious causes of food-borne illness around the world. its ability to persistently colonize the intestinal tract of a broad range of hosts, including food-producing animals, is central to its epidemiology since most infections are due to the consumption of contaminated food products. using a highly saturated transposon insertion library combined with next-generation sequencing and a mouse model of infection, we have carried out a comprehensive genome ...201728542173
comparing the genetic diversity and antimicrobial resistance profiles of campylobacter jejuni recovered from cattle and humans.campylobacter jejuni, a leading cause of gastroenteritis in humans, is a foodborne pathogen that can reside in chickens, pigs, and cattle. because resistance to fluoroquinolones and macrolides, which are commonly used to treat human infections, has emerged in c. jejuni, it is imperative to continously monitor resistance patterns and examine the genetic variation in strains from human infections and animal reservoirs. our previous study of c. jejuni from human campylobacteriosis cases showed a si ...201728536568
association of anti-gangliosides antibodies and anti-cmv antibodies in guillain-barré syndrome.numerous types of infection were closely related to gbs, mainly including campylobacter jejuni, cytomegalovirus, which may lead to the production of anti-gangliosides antibodies (aga). currently, although there are increased studies on the aga and a few studies of anti-cmv antibodies in gbs, the association between them remains poorly documented. therefore, our research aims to analyze the correlation of anti-cmv antibodies and aga in gbs.201728523231
lactobacillus johnsonii ameliorates intestinal, extra-intestinal and systemic pro-inflammatory immune responses following murine campylobacter jejuni infection.campylobacter jejuni infections are progressively increasing worldwide. probiotic treatment might open novel therapeutic or even prophylactic approaches to combat campylobacteriosis. in the present study secondary abiotic mice were generated by broad-spectrum antibiotic treatment and perorally reassociated with a commensal murine lactobacillus johnsonii strain either 14 days before (i.e. prophylactic regimen) or 7 days after (i.e. therapeutic regimen) peroral c. jejuni strain 81-176 infection. f ...201728522817
immunoprevalence to six waterborne pathogens in beachgoers at boquerón beach, puerto rico: application of a microsphere-based salivary antibody multiplex immunoassay.waterborne infectious diseases are a major public health concern worldwide. few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. in this article, we applied a multiplex bead-based immunoassay capable of ...201728507984
prevalence and characterization of campylobacter jejuni isolated from retail chicken in tianjin, china.campylobacter jejuni is an important foodborne pathogen worldwide; however, there is a lack of information on the prevalence and antibiotic-resistant profile of c. jejuni in the people's republic of china. we determined the prevalence and characteristics of c. jejuni on the retail level in tianjin, one of the five national central cities in china. a total of 227 samples of chicken wings, legs, and breasts were collected from supermarkets and wet markets; 42 of these samples were confirmed to be ...201728504616
identification of potential campylobacter jejuni genes involved in biofilm formation by ez-tn5 transposome mutagenesis.biofilm formation has been suggested to play a role in the survival of campylobacter jejuni in the environment and contribute to the high incidence of human campylobacteriosis. molecular studies of biofilm formation by campylobacter are sparse.201728499399
comparative proteomics reveals differences in host-pathogen interaction between infectious and commensal relationship with campylobacter jejuni.campylobacter jejuni is the leading food-borne poisoning in industrialized countries. while the bacteria causes disease in humans, it merely colonizes the gut in poultry or pigs, where seems to establish a commensal relationship. until now, few studies have been conducted to elucidate the relationship between c. jejuni and its different hosts. in this work, a comparative proteomics approach was used to identify the underlying mechanisms involved in the divergent outcome following c. jejuni infec ...201728491823
cj0440c affects flagella formation and in vivo colonization of erythromycin-susceptible and -resistant campylobacter jejuni.campylobacter jejuni is one of the most common foodborne pathogen worldwide. a putative transcriptional regulator, cj0440c, was up-regulated in the erythromycin-resistant c. jejuni, however, the precise role of cj0440c is yet to be determined. the aim of this study was to determine the biological functions of cj0440c. the cj0440c isogenic mutants were constructed from erythromycin-susceptible c. jejuni nctc 11168 (s) and -resistant c. jejuni 68-er (r), designating as sm and rm, respectively. the ...201728487689
trans-cinnamaldehyde, carvacrol, and eugenol reduce campylobacter jejuni colonization factors and expression of virulence genes in vitro.campylobacter jejuni is a major foodborne pathogen that causes severe gastroenteritis in humans characterized by fever, diarrhea, and abdominal cramps. in the human gut, campylobacter adheres and invades the intestinal epithelium followed by cytolethal distending toxin mediated cell death, and enteritis. reducing the attachment and invasion of campylobacter to intestinal epithelium and expression of its virulence factors such as motility and cytolethal distending toxin (cdt) production could pot ...201728487683
microbiota-derived short-chain fatty acids modulate expression of campylobacter jejuni determinants required for commensalism and virulence.campylobacter jejuni promotes commensalism in the intestinal tracts of avian hosts and diarrheal disease in humans, yet components of intestinal environments recognized as spatial cues specific for different intestinal regions by the bacterium to initiate interactions in either host are mostly unknown. by analyzing a c. jejuni acetogenesis mutant defective in converting acetyl coenzyme a (ac-coa) to acetate and commensal colonization of young chicks, we discovered evidence for in vivo microbiota ...201728487428
epidemiology of antimicrobial resistant campylobacter spp. isolated from retail meats in canada.campylobacter is an important zoonotic pathogen found in livestock and can cause illness in humans following consumption of raw and undercooked meat products. the objectives of this study were to determine the prevalence of campylobacter spp. in retail meat (poultry, turkey, pork and beef) purchased in alberta, canada and to assess antimicrobial resistance and genetic relatedness of recovered campylobacter strains with previously isolated strains from clinical and environmental sources. a compar ...201728477522
high prevalence and predominance of the aph(2″)-if gene conferring aminoglycoside resistance in campylobacter.campylobacter is a major foodborne pathogen and previous studies revealed that campylobacter isolates from food-producing animals are increasingly resistant to gentamicin in china. the molecular epidemiology and genetic mechanisms responsible for gentamicin resistance in china have not been well understood. in this study, 607 campylobacter isolates of chicken and swine origins collected in the year of 2014 were analyzed, revealing that 15.6% (25/160) of the c. jejuni isolates and 79.9% (357/447) ...201728264854
high rates of contamination of poultry meat products with drug resistant campylobacter in metro manila, philippines.a total of 265 chicken parts were collected from 15 wet markets and 15 supermarkets in metro manila, philippines. campylobacter spp. was isolated on modified charcoal cefoperazone deoxycholate agar plates and identified through biochemical tests and pcr amplification of genus and species specific genes. antimicrobial resistance profiles were determined following clinical and laboratory standards institute protocols. two hundred seven (78%) campylobacter spp. were isolated. c. jejuni and c. coli ...201627795478
causality assessment of serious neurologic adverse events following bopv national vaccination campaign in israel.during 2013-2014 israel experienced a continuous circulation of wild poliovirus type 1 (wpv1) but with no clinical cases. wpv1 circulation was gradually terminated following a national vaccination campaign of bivalent oral poliovirus vaccine (bopv) for 943,587 children < 10 years. four cases of children with neurological manifestations that appeared following bopv vaccinations were reported during the campaign: three of guillain-barré syndrome (gbs) and one of acute disseminated encephalomyeliti ...201628471617
oxidative and nitrosative stress defences of helicobacter and campylobacter species that counteract mammalian immunity.helicobacter and campylobacter species are gram-negative microaerophilic host-associated heterotrophic bacteria that invade the digestive tract of humans and animals. campylobacter jejuni is the major worldwide cause of foodborne gastroenteritis in humans, while helicobacter pylori is ubiquitous in over half of the world's population causing gastric and duodenal ulcers. the colonisation of the gastrointestinal system by helicobacter and campylobacter relies on numerous cellular defences to sense ...201628201757
the effect of microbial challenge on the intestinal proteome of broiler chickens.in poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. uncovering the localised molecular mechanisms that occur during the early and important periods of growth that allow birds to grow optimally is important for this species. the exposure of young chicks to used litter from older flocks, containing mixed microbial populations, is a widely utilised model in poultry research. it rarely causes mortality but effects an immunogenic stimu ...201628572745
septic abortion caused by campylobacter jejuni bacteraemia.a 20-year-old female patient, 14 weeks pregnant, was admitted to hospital with anamnestic and clinical features of acute pyelonephritis. clinical signs of septic abortion developed and after obstetric examination the therapy was changed to ampicillin, gentamicin and clindamycin. campylobacter jejuni was isolated from blood cultures. pathohistological findings confirmed diagnosis of purulent chorioamnionitis. after 2 weeks of ciprofloxacin administration the patient fully recovered. campylobacter ...201625872616
application of bacteriophages in post-harvest control of human pathogenic and food spoiling bacteria.bacteriophages have attracted great attention for application in food biopreservation. lytic bacteriophages specific for human pathogenic bacteria can be isolated from natural sources such as animal feces or industrial wastes where the target bacteria inhabit. lytic bacteriophages have been tested in different food systems for inactivation of main food-borne pathogens including listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, salmonella enterica, shigella spp., campylobac ...201626042353
hyperimmunised bovine milk and whey: influence of ph and enzymatic treatments on the antigen-binding capacity of immunoglobulin g.hyperimmunised bovine milk and whey (whole and defatted) were submitted at 37°c to different ph values (between 1 and 10) and enzymes (pepsin, trypsin and chymotrypsin) at their optimum ph and the igg immunoactivity against campylobacter jejuni was measured by means of elisa assays.201626041450
anti-adhesion activity of thyme (thymus vulgaris l.) extract, thyme post-distillation waste, and olive (olea europea l.) leaf extract against campylobacter jejuni on polystyrene and intestine epithelial cells.in order to survive in food-processing environments and cause disease, campylobacter jejuni requires specific survival mechanisms, such as biofilms, which contribute to its transmission through the food chain to the human host and present a critical form of resistance to a wide variety of antimicrobials.201626304165
an improved multiplex real-time sybr green pcr assay for analysis of 24 target genes from 16 bacterial species in fecal dna samples from patients with foodborne illnesses.here, we developed a new version of our original screening system (rapid foodborne bacterial screening 24; rfbs24), which can simultaneously detect 24 genes of foodborne pathogens in fecal dna samples. this new version (rfbs24 ver. 5) detected all known stx2 subtypes, enterotoxigenic escherichia coli (sth genotype), and vibrio parahaemolyticus (trh2), which were not detected by the original rfbs24 assay. the detection limits of rfbs24 ver. 5 were approximately 5.6 × 10(-2)-5.6 × 10(-5) (ng dna)/ ...201626166499
antibiotic resistance of campylobacter jejuni and c. coli isolated from children with diarrhea in thailand and japan.a total of 29 campylobacter jejuni and c. coli strains were isolated from thai and japanese children with diarrhea using the loop-mediated isothermal amplification method. the samples were evaluated for mutations in gyra and 23s rrna in order to assess resistance against fluoroquinolones and macrolides, respectively. among the isolated strains, 9 (8 c. jejuni and 1 c. coli) were from thai children, and the other 20 (c. jejuni) were isolated from japanese children. high fluoroquinolone resistance ...201626166497
monomerization alters the dynamics of the lid region in campylobacter jejuni cstii: an md simulation study.cstii, a bifunctional (α2,3/8) sialyltransferase from campylobacter jejuni, is a homotetramer. it has been reported that mutation of the interface residues phe121 (f121d) or tyr125 (y125q) leads to monomerization and partial loss of enzyme activity, without any change in the secondary or tertiary structures. md simulations of both tetramer and monomer, with and without bound donor substrate, were performed for the two mutants and wt to understand the reasons for partial loss of activity due to m ...201626208676
psychological comorbidity increases the risk for postinfectious ibs partly by enhanced susceptibility to develop infectious gastroenteritis.psychological factors increase the risk to develop postinfectious ibs (pi-ibs), but the mechanisms involved are unclear. as stress affects the immune system, we investigated the potential interaction between psychological factors, the immune response against infectious gastroenteritis (ige) and the development of ige and pi-ibs in a large cohort exposed to contaminated drinking water.201626071133
campylobacter-associated hemolytic uremic syndrome associated with pulmonary-renal syndrome.common causes of pulmonary-renal syndrome include anti-glomerular basement membrane (anti-gbm) disease anti-neutrophil cytoplasmic antibody (anca) positive vasculitis, and systemic lupus erythematosus. we describe a case of life-threatening pulmonary hemorrhage associated with campylobacter hemolytic uremic syndrome (hus), which we believe is a new disease entity. we hypothesize that the cause of this pulmonary-renal syndrome was an immunological reaction to campylobacter; and that the initiatio ...201626001543
antimicrobial resistance and multilocus sequence types of finnish campylobacter jejuni isolates from multiple sources.antimicrobial susceptibility was determined for 805 domestic campylobacter jejuni isolates obtained from broilers (n = 459), bovines (n = 120), human patients (n = 95), natural waters (n = 80), wild birds (n = 35) and zoo animals/enclosures (n = 16) with known multilocus sequence types (mlst) for 450 isolates. the minimum inhibitory concentration (mic) values for erythromycin, tetracycline, streptomycin, gentamicin and the quinolones ciprofloxacin and nalidixic acid were determined with the vetm ...201625917650
composition of human faecal microbiota in resistance to campylobacter infection.in mice, specific species composition of gut microbiota enhances susceptibility to campylobacter jejuni but little is known about the specific composition of the human gut microbiota in providing protection from infections caused by enteropathogens. healthy adult individuals, who travelled in groups from sweden to destinations with an estimated high risk for acquisition of campylobacter infection, were enrolled. faecal samples, collected before travelling and after returning home, were cultured ...201626369602
seasonality of campylobacter jejuni isolates associated with human campylobacteriosis in the manawatu region, new zealand.a 9-year time-series of genotyped human campylobacteriosis cases from the manawatu region of new zealand was used to investigate strain-type seasonality. the data were collected from 2005 to 2013 and the samples were multi-locus sequence-typed (mlst). the four most prevalent clonal complexes (ccs), consisting of 1215 isolates, were cc48, cc21, cc45 and cc61. seasonal decomposition and poisson regression with autocorrelated errors, were used to display and test for seasonality of the most prevale ...201626344515
a cytolethal distending toxin gene-based multiplex pcr assay for campylobacter jejuni, c. fetus, c. coli, c. upsaliensis, c. hyointestinalis, and c. lari.in this study, we devised a multiplex pcr assay based on the gene of cytolethal distending toxin (cdt) b subunit to simultaneously detect and discriminate campylobacter jejuni, c. fetus, c. coli, c. upsaliensis, c. hyointestinalis, and c. lari. species-specific pcr products were successfully obtained from all 38 c. jejuni, 12 c. fetus, 39 c. coli, 22 c. upsaliensis, 24 c. hyointestinalis, and 7 c. lari strains tested. on the other hand, no specific pcr products were obtained from other campyloba ...201626255737
campylobacter jejuni outer membrane vesicle-associated proteolytic activity promotes bacterial invasion by mediating cleavage of intestinal epithelial cell e-cadherin and occludin.outer membrane vesicles (omvs) play an important role in the pathogenicity of gram-negative bacteria. campylobacter jejuni produces omvs that trigger il-8, il-6, hbd-3 and tnf-α responses from t84 intestinal epithelial cells and are cytotoxic to caco-2 iecs and galleria mellonella larvae. proteomic analysis of 11168h omvs identified the presence of three proteases, htra, cj0511 and cj1365c. in this study, 11168h omvs were shown to possess proteolytic activity that was reduced by pretreatment wit ...201626451973
molecular typing of fluoroquinolone-resistant campylobacter jejuni isolated from broilers in japan using multilocus sequence typing and pulsed-field gel electrophoresis.fluoroquinolone-resistant campylobacter jejuni isolates from broilers in japan were characterized using multilocus sequence typing and pulsed-field gel electrophoresis (pfge) in order to elucidate the genetic relationship between these strains. forty-three of the isolates were classified into 20 sequence types and were clustered into 21 pfge types with 70% similarity. the most dominant clonal complex (cc) was cc-21 (41.9%). diverse pfge patterns were observed within the same cc, but the combined ...201626447604
pathogen loading from canada geese faeces in freshwater: potential risks to human health through recreational water exposure.canada geese (branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. further, increases in both the canada geese populations and their ideal habitat requirements in the united states (us) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. combining these factors, the potential health risk posed by canada geese faeces at freshwater beaches presents an emerging ...201626414207
variation in the limit-of-detection of the prospect campylobacter microplate enzyme immunoassay in stools spiked with emerging campylobacter species.campylobacter enteritis in humans is primarily associated with c. jejuni/coli infection. the impact of other campylobacter spp. is likely to be underestimated due to the bias of culture methods towards campylobacter jejuni/coli diagnosis. stool antigen tests are becoming increasingly popular and appear generally less species-specific. a review of independent studies of the prospect® campylobacter microplate enzyme immunoassay (eia) developed for c. jejuni/coli showed comparable diagnostic result ...201627317896
three transcription regulators of the nss family mediate the adaptive response induced by nitrate, nitric oxide or nitrous oxide in wolinella succinogenes.sensing potential nitrogen-containing respiratory substrates such as nitrate, nitrite, hydroxylamine, nitric oxide (no) or nitrous oxide (n2 o) in the environment and subsequent upregulation of corresponding catabolic enzymes is essential for many microbial cells. the molecular mechanisms of such adaptive responses are, however, highly diverse in different species. here, induction of periplasmic nitrate reductase (nap), cytochrome c nitrite reductase (nrf) and cytochrome c n2 o reductase (cnos) ...201626395430
antibiotic susceptibility and molecular characterization of campylobacter jejuni strain isolated from a guillain barré syndrome child. 201626558915
conserved histidine residues at the ferroxidase centre of the campylobacter jejuni dps protein are not strictly required for metal binding and oxidation.iron is an essential micronutrient for living organisms as it is involved in a broad variety of important biological processes. however, free iron inside the cell could be potentially toxic, generating hydroxyl radicals through the fenton reaction. dps (dna-binding protein from starved cells) belongs to a subfamily of ferritins and can store iron atoms inside the dodecamer. the presence of a ferroxidase centre, composed of highly conserved residues, is a signature of this protein family. in this ...201626555736
control strategies against campylobacter at the poultry production level: biosecurity measures, feed additives and vaccination.campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the european union, and ranks second in the united states only behind salmonellosis. in europe, there are about nine million cases of campylobacteriosis every year, making the disease a major public health issue. human cases are mainly caused by the zoonotic pathogen campylobacter jejuni. the main source of contamination is handling or consumption of poultry meat. poultry constitutes the main reservo ...201626541243
ex vivo proteomics of campylobacter jejuni 81-176 reveal that fabg affects fatty acid composition to alter bacterial growth fitness in the chicken gut.campylobacter jejuni is one of the leading causes of foodborne gastrointestinal illness worldwide. here we performed ex vivo proteomic analysis of c. jejuni 81-176 in chicken, a main reservoir for human infection. at 0, 1 and 4 weeks post-infection (p.i.) with the gfp-expressing 81-176 strain, inocula were recovered from chicken ceca by cell sorting using flow cytometry. itraq-coupled 2d-lc-ms/ms analyses that detected 55 c. jejuni proteins, among which either 3 (fabg, hydb, cjj81176_0876) or 7 ...201626499093
major contribution of the type ii beta carbonic anhydrase canb (cj0237) to the capnophilic growth phenotype of campylobacter jejuni.campylobacter jejuni, the leading cause of human bacterial gastroenteritis, requires low environmental oxygen and high carbon dioxide for optimum growth, but the molecular basis for the carbon dioxide requirement is unclear. one factor may be inefficient conversion of gaseous co2 to bicarbonate, the required substrate of various carboxylases. two putative carbonic anhydrases (cas) are encoded in the genome of c. jejuni strain nctc 11168 (cj0229 and cj0237). here, we show that the deletion of the ...201626470757
seroprevalence in chickens against campylobacter jejuni flagellar capping protein (flid) in selected areas of the united states.campylobacter jejuni is a causative pathogen of human acute bacterial gastroenteritis. infected poultry products are regarded as a major source for human c. jejuni infection. the flagellar capping protein (flid) is highly conserved among c. jejuni strains/isolates and is antigenic as analysed by immunoblot. in this study, we used the flid protein as a probe to survey the prevalence of c. jejuni antibodies in chickens from two areas in the united states. a total of 394 samples were tested. sera f ...201626603949
a seventeen-year observation of the antimicrobial susceptibility of clinical campylobacter jejuni and the molecular mechanisms of erythromycin-resistant isolates in beijing, china.to investigate the dynamic development of the antimicrobial resistance of campylobacter jejuni isolated from human diarrhea in beijing, china, between 1994 and 2010, and to further analyze the molecular mechanisms of erythromycin-resistant strains.201626594011
diversity and relatedness of shiga toxin-producing escherichia coli and campylobacter jejuni between farms in a dairy catchment.the aim of this study was to examine the population structure, transmission and spatial relationship between genotypes of shiga toxin-producing escherichia coli (stec) and campylobacter jejuni, on 20 dairy farms in a defined catchment. pooled faecal samples (n = 72) obtained from 288 calves were analysed by real-time polymerase chain reaction (rtpcr) for e. coli serotypes o26, o103, o111, o145 and o157. the number of samples positive for e. coli o26 (30/72) was high compared to e. coli o103 (7/7 ...201626593403
phenotypic characters and molecular epidemiology of campylobacter jejuni in east china.in this study, we investigated the distribution, phenotypic and molecular typing characters of campylobacter jejuni in domestic fowl, and livestock populations in east china, to provide some reference for researches on its molecular epidemiology. a total of 1250 samples were collected from different animal sources, and c. jejuni strains were then isolated and tested for antibiotic sensitivity. antibiotics-resistance gene and pathogenic genes were detected by polymerase chain reaction. phylogenic ...201626565657
campylobacter species isolated from poultry and humans, and their analysis using pfge in southern brazil.campylobacteriosis is a bacterial disease transmitted to humans through ingestion of contaminated food. six hundred samples were collected, 200 from human stool samples, 200 from poultry products and 200 from poultry feces in southern brazil, and then inoculated on blood agar plates. a total of 58% of the poultry feces, 17% of the poultry meat, and 2% the of human stools tested positive for campylobacter. positive campylobacter colonies were identified as campylobacter jejuni or campylobactercol ...201626561789
molecular and epidemiological analysis of a campylobacter jejuni outbreak in northern italy in november 2013.campylobacter spp. is the most common gastrointestinal pathogen worldwide with a very low reported incidence in italy. in november of 2013, local and national public health authorities investigated an outbreak caused by campylobacter jejuni among children in a kindergarten in northern italy. a case was defined as a child who had diarrhea with a laboratory-confirmed diagnosis of c. jejuni between 11 and 30 november. stool samples from the kindergarten kitchen staff and environmental samples from ...201627455195
conformational dynamics of peb4 exhibit "mother's arms" chain model: a molecular dynamics study.peb4 from campylobacter jejuni is an intertwined dimeric, periplasmic holdase, which also exhibits peptidyl prolyl cis/trans isomerase (ppiase) activity. peb4 gene deletion alters the outer membrane protein profile and impairs cellular adhesion and biofilm formation for c. jejuni. earlier crystallographic study has proposed that the ppiase domains are flexible and might form a cradle for holding the substrate and these aspects of peb4 were explored using sub-microsecond molecular dynamics simula ...201627434141
immunoproliferative small intestinal disease associated with overwhelming polymicrobial gastrointestinal infection with transformation to diffuse large b-cell lymphoma.immunoproliferative small intestinal disease (ipsid) is an extra-nodal b-cell lymphoma most commonly described in the mediterranean, africa, and asia. it is associated with poverty and poor sanitation, and is rarely encountered in developed countries. a 26-year-old previously healthy, marshallese male was transferred to our facility with a 6-month history of watery diarrhea, weakness, and cachexia refractory to multiple short courses of oral antibiotics. stool cultures grew campylobacter jejuni ...201626903604
bayesian techniques for comparison of the test performance of pcr and culture for the identification of campylobacter in enriched comminuted chicken samples.using bayesian methods that do not require the definition of a gold standard, the diagnostic sensitivity and specificity of a real-time polymerase chain reaction (pcr) assay are compared to those of an enriched culture assay for detection of campylobacter in enriched comminuted chicken samples.201626895234
the fecal microbial communities of dairy cattle shedding shiga toxin-producing escherichia coli or campylobacter jejuni.cattle are a natural reservoir of shiga toxin-producing escherichia coli (stec) and have recently been recognized as a major source of campylobacter jejuni contamination. while several factors are known to be associated with bacterial colonization, the underlying microbial factors have not been clarified. in this study, we characterized the fecal microbiota of dairy cattle (n = 24) using next-generation sequencing to elucidate the intestinal bacterial communities and the microbial diversity in r ...201627385033
travelers lowering their guard: a bacterial, viral and protozoan co-infection after a five-day journey in india.we present a case of concurrent infections by campylobacter jejuni, giardia intestinalis and hepatitis e virus acquired during a 5-days travel to india by an italian traveller : professionals responsible for pre- and post-travel care should underline food and water precautions and prescribe an adequate diagnostic work-up in symptomatic patients.201627378368
[research progress in biofilm formation and regulatory mechanism of campylobacter jejuni].biofilm of campylobacter jejuni was formed by cross-linking its extracellular secretion, polysaccharides, various extracellular proteins, nucleic acids etc to enhance its survival in hostile environments, especially for detergents, antibiotics and disinfectants. this paper elaborated c. jejuni biofilm formation and regulation mechanisms in the surface properties of the media, temperatures, gas environment, the regulation of gene etc, also analysed and discussed a variety of biofilm removal pract ...201627373066
feedback control of campylobacter jejuni flagellin levels through reciprocal binding of fliw to flagellin and the global regulator csra.bacterial flagella assembly is tightly regulated to ensure a timely and sequential production of the various flagellum constituents. in the pathogen campylobacter jejuni the hierarchy in flagella biosynthesis is largely determined at the transcriptional level through the activity of the alternative sigma factors sigma(54) and sigma(28) . here, we report that c. jejuni flagellin levels are also controlled at the post-transcriptional level via the thus far poorly-characterized flagellar assembly f ...201627353476
adaptation of campylobacter jejuni to biocides used in the food industry affects biofilm structure, adhesion strength, and cross-resistance to clinical antimicrobial compounds.the emergence of biocide-adapted campylobacter jejuni strains that developed into biofilms and their potential to develop clinical resistance to antimicrobial compounds was studied. c. jejuni was grown in sub-lethal concentrations of five biocides used in the food industry. c. jejuni exhibited adaptation to these biocides with increased minimum inhibitory concentrations. the 3-d structures of the biofilms produced by the biocide-adapted cells were investigated by atomic force microscopy (afm). t ...201627353218
campylobacter jejuni infections associated with raw milk consumption--utah, 2014.in may 2014, the utah public health laboratory (uphl) notified the utah department of health (udoh) of specimens from three patients infected with campylobacter jejuni yielding indistinguishable pulsed-field gel electrophoresis (pfge) patterns. all three patients had consumed raw (unpasteurized and nonhomogenized) milk from dairy a. in utah, raw milk sales are legal from farm to consumer with a sales permit from the utah department of agriculture and food (udaf). raw milk dairies are required to ...201627031585
efficacy of neutral electrolyzed water, quaternary ammonium and lactic acid-based solutions in controlling microbial contamination of food cutting boards using a manual spraying technique.bactericidal activity of neutral electrolyzed water (new), quaternary ammonium (quat), and lactic acid-based solutions was investigated using a manual spraying technique against salmonella typhimurium, escherichia coli o157:h7, campylobacter jejuni, listeria monocytogenes and staphylococcus aureus that were inoculated onto the surface of scarred polypropylene and wooden food cutting boards. antimicrobial activity was also examined when using cutting boards in preparation of raw chopped beef, chi ...201627027449
temporal variation in the prevalence and species richness of campylobacter spp. in a prairie watershed impacted by urban and agricultural mixed inputs.campylobacter spp. are a substantial cause of gastroenteritis worldwide. human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. using molecular methods, we investigated the occurrence of campylobacter in the qu'appelle river watershed, an important source of irrigation water for vegetable producers in southern saskatchewan, ca ...201627003220
detection of campylobacter in human and animal field samples in cambodia.campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with campylobacter jejuni and c. coli being the most commonly detected species. the aim of this study was to detect campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex pcr in faecal samples frozen before analysis. of 681 human samples, 82 (12%) tested positive by pcr (c. jejuni in 66 samples an ...201626991032
de novo asymmetric synthesis of a 6-o-methyl-d-glycero-l-gluco-heptopyranose-derived thioglycoside for the preparation of campylobacter jejuni nctc11168 capsular polysaccharide fragments.an enantioselective de novo synthesis of a thioglycoside derivative of the 6-o-methyl-d-glycero-l-gluco-heptopyranose residue found in the campylobacter jejuni nctc11168 (hs:2) capsular polysaccharide is reported. the compound is obtained from a furfural-derived chiral diol in 11 steps. notably, compared to the only previous synthesis of this molecule, this approach significantly reduces the number of purification steps required to obtain the target.201626982173
status of vaccine research and development for campylobacter jejuni.campylobacter jejuni is one of the leading causes of bacterial diarrhea worldwide and is associated with a number of sequelae, including guillain-barre syndrome, reactive arthritis, irritable bowel syndrome and growth stunting/malnutrition. vaccine development against c. jejuni is complicated by its antigenic diversity, a lack of small animal models, and a poor understanding of the bacterium's pathogenesis. vaccine approaches have been limited to recombinant proteins, none of which have advanced ...201626973064
validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products.this study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, campylobacter jejuni, listeria monocytogenes, and salmonella spp., for its actual application in routine food testing. due to the limitation of the detection limit of the developed method, an enrichment step was included in this study by using campylobacter enrichment broth for c. jejuni and universal pre-enrichment broth for l. monocytogenes and salmonella ...201626950032
fluoroquinolone and macrolide resistance in campylobacter jejuni isolated from broiler slaughterhouses in southern brazil.campylobacter jejuni is recognized as a leading cause of acute bacterial gastroenteritis in humans. the over-use of antimicrobials in the human population and in animal husbandry has led to an increase in antimicrobial-resistant infections, particularly with fluoroquinolones and macrolides. the aim of the present study was to provide information of the current status of antimicrobial resistance patterns in campylobacter jejuni from poultry sources. fifty strains were recovered from broiler slaug ...201626925976
anti-gq1b antibody syndrome presenting as acute isolated bilateral ophthalmoplegia: report on two patients and review of the literature.miller fisher syndrome (mfs) is an acute polyradiculoneuritis regarded as an uncommon clinical variant of guillain-barré syndrome (gbs). mfs is characterized by the acute onset of the clinical triad of ophthalmoplegia, cereballar ataxia and areflexia. atypical forms of mfs presenting as isolated ophthalmoplegia without ataxia have been rarely described, mostly in adults.201626924168
the crystal structure of the tandem-pas sensing domain of campylobacter jejuni chemoreceptor tlp1 suggests indirect mechanism of ligand recognition.chemotaxis and motility play an important role in the colonisation of avian and human hosts by campylobacter jejuni. chemotactic recognition of extracellular signals is mediated by the periplasmic sensing domain of methyl-accepting chemotactic proteins (membrane-embedded receptors). in this work, we report a high-resolution structure of the periplasmic sensing domain of transducer-like protein 1 (tlp1), an aspartate receptor of c. jejuni. crystallographic analysis revealed that it contains two p ...201626923153
probing polytopic membrane protein-substrate interactions by luminescence resonance energy transfer.integral membrane proteins play essential roles in all living systems; however, major technical hurdles challenge analyses of this class of proteins. biophysical approaches that provide structural information to complement and leverage experimentally determined and computationally predicted structures are urgently needed. herein we present the application of luminescence resonance energy transfer (lret) for investigating the interactions of the polytopic membrane-bound oligosaccharyl transferase ...201626918528
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