Publications

a modified broth-disk antibiotic susceptibility test for campylobacter jejuni.	a broth-disk method for determining the antibiotic susceptibility of campylobacter species was studied. the broth-disk method used to test anaerobes was modified for testing the antibiotic susceptibility of campylobacter jejuni in an aerobic atmosphere. the basal medium of the test consisted of brucella broth with 0.13% agar and contained a ferrous iron, sodium pyruvate, sodium bisufite supplement. antibiotic disks were added to the tubed medium to approximate the minimal inhibitory concentratio ...	1982	7160369
campylobacter jejuni enteritis in man and domestic animals.		1982	7161171
campylobacter jejuni contamination of slaughtered chickens.	gut samples from 50 nonselected slaughtered chickens were obtained in two poultry processing plants and cultured for campylobacter jejuni and salmonella. positive results were obtained in 84% and 4%, respectively. viable c. jejuni and salmonella were detected in every phase of processing, even during packaging for commercial purposes. of surface samples taken from 118 slaughtered chickens prepared for delivery to consumers, 88 were contaminated by c. jejuni and 17 by salmonella.	1982	7168372
campylobacter jejuni enteritis: incidence, age distribution and clinical symptoms.		1982	7168373
[the occurrence of campylobacter jejuni in rabbits, guinea pigs, rats and mice in the laboratory animal unit].		1982	7168755
survival of campylobacter jejuni inoculated into ground beef.	ground beef was inoculated with mixed cultures of campylobacter jejuni, and the samples were subjected to various cooking and cold-storage temperatures. when samples were heated in an oven at either 190 or 218 degrees c, approximately 10(7) cells of c. jejuni per g were inactivated (less than 30 cells per g) in less than 10 min after the ground beef reached an internal temperature of 70 degrees c. when the samples were held at -15 degrees c over 14 days of storage, the numbers of c. jejuni decli ...	1982	7181502
[morphological differences between campylobacter jejuni and c. fetus].		1982	7182541
campylobacter in healthy slaughter pigs: a possible source of infection for man.	campylobacter were isolated from 103 of 173 (59 per cent) specimens of healthy slaughter pig faeces, washed intestines and water samples collected from a slaughterhouse and butcher's shop in west germany. as most cases of human campylobacter enteritis are caused by campylobacter jejuni, an attempt was made to find this organism among the isolates. twenty-five out of the 103 strains (24 per cent) were identified as c jejuni. c jejuni was also isolated from salted water samples after overnight bow ...	1982	7186689
campylobacter enteritis: a large outbreak traced to commercial raw milk.	from april 24 to may 11, 1981, an outbreak of approximately 200 cases of campylobacter jejuni enteritis occurred in arizona in persons who drank one brand of unpasteurized milk. two cohort studies showed that households with members who drank raw milk reported diarrheal illness significantly more frequently than those in which no one drank raw milk (p=.003 and p=.001; relative risk 4.70 and 3.85, respectively). of 19 serotyped c jejuni organisms isolated from persons who drank raw milk from the ...	1982	7179958
effect of recovery medium on the isolation of campylobacter jejuni before and after heat treatment.		1982	7180785
campylobacter jejuni: incidence in processed broilers and biotype distribution in human and broiler isolates.	campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in sydney, australia. three of four flocks examined carried c. jejuni. eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes.	1982	7201780
comparison of four enrichment media in the recovery of campylobacter jejuni.		1982	7180786
[campylobacter jejuni enteritis in childhood. apropos of 3 cases].		1982	7181396
campylobacter jejuni and salmonella in raw red meats: a public health laboratory service survey.	thirty-one laboratories examined a total of 6169 meat samples, 1236 from abattoirs and 4933 from retail and other outlets. campylobacter jejuni was isolated from 98 (1.6%). a higher isolation rate of 49/1236 (4.0%) was found among abattoir than among retail and other samples (49/4933-1.0%). twenty-two of the laboratories looked for salmonella; although 94/4002 (2.3%) were positive, in only one sample of minced beef were campylobacter and salmonella found together. isolation rates for salmonellae ...	1982	20475890
waterborne transmission ofcampylobacter enteritis.	campylobacter jejuni is an important cause of human diarrheal disease throughout the world and likesalmonella enteritidis, has a large animal reservoir which includes most of man's domestic animals. until recently, it has been difficult to trace the chain of transmission from animals to man because of inadequate environmental sampling techniques and means to distinguish strains. recent improvements in these techniques have made environmental studies more feasible in 2 water-related out-breaks.in ...	1982	24226052
[comparative use of 2 selective culture media for isolation of campylobacter jejuni from animal fecal samples].		1983	6868879
ferret as a potential reservoir for human campylobacteriosis.	a survey was conducted to determine the frequency of campylobacter fetus subsp jejuni in feces of ferrets purchased for use in biomedical research. over a 12-month period, 168 ferrets from 2 commercial breeders were examined by bacteriologic cultural procedures for campylobacter spp; 61% were culturally positive for c fetus subsp jejuni. in a therapeutic trial with 16 ferrets shedding c fetus subsp jejuni in feces, erythromycin given orally failed to eliminate intestinal carriage of the organism ...	1983	6870007
susceptibility of clinical isolates of campylobacter jejuni to sixteen antimicrobial agents.	the activities of 16 antimicrobial agents against 103 clinical isolates of campylobacter jejuni were tested. all the strains were susceptible to kanamycin and gentamicin. chloramphenicol, nalidixic acid, and clindamycin were active against most of the strains. more than one-third of the strains were resistant to the tetracyclines and 12.5% were resistant to erythromycin.	1983	6870228
[reactive arthritis associated with campylobacter jejuni enteritis].		1983	6845487
isolation of campylobacter jejuni from an aborted caprine fetus.	campylobacter jejuni was isolated in pure culture from the tissues and stomach contents of an aborted fetus from a mixed-breed illinois goat herd. in the herd, 5 of 21 does aborted in late gestation. the does had diarrhea before or concurrent with the abortions. does that did not abort were vaccinated with ovine c fetus bacterin and were given chlortetracycline orally at the rate of 75 mg/day for 2 weeks. further abortions did not occur.	1983	6874530
ascorbic acid inhibition of campylobacter jejuni growth.	the inhibitory effect of ascorbic acid on campylobacter jejuni is described. in vitro growth of clinical strains, as measured spectrophotometrically, was inhibited by 0.5 mg of freshly prepared l-ascorbic acid per ml. alkaline-treated or aged l-ascorbic acid increased inhibition, as did copper; however, l-cysteine, l-cystine, and glutathione prevented inhibition. biochemical analysis of the medium and cultures indicated that one or more of the oxidation products of l-ascorbic acid, e.g., l-dehyd ...	1983	6847185
[occurrence of campylobacter jejuni in cats].		1983	6847593
role of clostridium difficile and campylobacter jejuni in relapses of inflammatory bowel disease.	findings from recent studies on the role of clostridium difficile and campylobacter jejuni in exacerbations of inflammatory bowel disease are in conflict. we examined stool specimens from 32 patients who had inflammatory bowel disease in relapse for the presence of c difficile and c difficile cytotoxin. in the last 19 cases stool specimens were also cultured for c jejuni. c difficile was identified in one patient who was receiving antimicrobial therapy. in no patient was c difficile cytotoxin de ...	1983	6858121
campylobacter enteritis from untreated water in the rocky mountains.	during the summers of 1980 and 1981 campylobacter jejuni was isolated from 23% and giardia lamblia was isolated from 8% of persons with diarrheal disease acquired in the area of grand teton national park, wyoming. campylobacter enteritis occurred most frequently in young adults who had been hiking in wilderness areas and was significantly associated with drinking untreated surface water in the week before illness (p less than 0.02 in 1980; p less than 0.005 in 1981). penner serotype 4 was the co ...	1983	6859722
intestinal occurrence of campylobacter jejuni in a french hospitalized population.		1983	6861735
location of campylobacter jejuni in infected chicken livers.	to determine whether chicken livers infected with campylobacter jejuni are seeded in vivo or contaminated after slaughtering, 117 livers purchased in retail outlets in new york were examined for surface and tissue infections. of 56 livers positive for c. jejuni, 36 yielded surface growth only, 18 both surface and tissue growth, and 2 tissue growth only. the scanty growth from tissue samples suggests a carry-over of organisms from the surface. it was concluded, therefore, that contamination is mo ...	1983	6863511
profuse diarrhea induced by campylobacter.	a 31-year-old woman with a five-day prodrome of fever, chills, and diarrhea was hospitalized with severe dehydration and profuse diarrhea due to infection with campylobacter jejuni. stool volumes were as high as 8,800 ml per 24 hours. treatment with intravenous fluids, trimethoprim-sulfamethoxazole, and an oral isotonic glucose-sodium solution produced rapid improvement, suggesting a possible role for oral fluid and electrolyte replacement in campylobacter enteritis.	1983	6867812
isolation of campylobacter fetus subsp. jejuni from the common puffin (fratercula arctica) in norway.		1983	6842738
role of dna and bacteriophage in campylobacter auto-agglutination.	auto-agglutinated and non-agglutinated cells of campylobacter jejuni and c. coli were examined by transmission electronmicroscopy in phosphotungstate negative stain. agglutination was induced by three factors (1) extracellular dna, (2) an aggregated protein, probably a bacteriophage precursor, and (3) free phage-tail sheaths. auto-agglutinated cells were often "leaky," with a mantle of adhering dna. about 80% of the auto-agglutinated cells could be resuspended after treatment with dnaase. flagel ...	1983	6876138
campylobacter jejuni isolated from patas monkeys with diarrhea.	campylobacter jejuni was isolated from 11 (46%) of 24 patas monkeys with chronic diarrhea. eight of these 11 (73%) monkey were characterized clinically by mucohemorrhagic diarrhea for periods up to a month followed by loose, semi-formed feces for a 12-month period. half of the monkeys were treated with erythromycin for 10 days and the other half with tetracycline for 10 days, with all responding to treatment. despite treatment, all monkeys again had an outbreak of mucohemorrhagic diarrhea. biops ...	1983	6876739
[isolation of campylobacter jejuni from children with acute gastroenteritis in belem, pará (preliminary report)].		1983	6879053
effect of incubation atmosphere and temperature on isolation of campylobacter jejuni from human stools.	to determine the optimal conditions for isolation of campylobacter jejuni from human fecal specimens, we compared incubation atmospheres that contained about 5, 10, and 15% oxygen with the 17% oxygen produced in candle jars and also compared incubation temperatures of 37 and 42 degrees c. at 42 degrees c, c. jejuni was isolated from all 16 specimens; however, colony sizes were larger when plates were incubated in 5 and 10% oxygen than in the other two atmospheres. at 37 degrees c some positive c ...	1983	6850426
an outbreak of campylobacter enteritis among the staff of a poultry abattoir in sweden.	in the middle of june 1980 an explosive outbreak of campylobacter enteritis occurred among the staff of a poultry abattoir in southern sweden. in all 37 cases of acute gastroenteritis originating from the abattoir were reported and campylobacter jejuni was isolated from the stools in 24 of them. when the outbreak occurred, a large proportion of the ordinary staff had been replaced by inexperienced teenagers working during their holidays. a specially big slaughter had also taken place the same we ...	1983	6879114
epidemiologic and clinical features of endemic campylobacter jejuni infection in bangladesh.	epidemiologic and clinical features of infection with campylobacter jejuni in bangladesh were examined in (1) diarrheal patients infected with c jejuni, (2) healthy control subjects, and (3) village children who were cultured monthly and at each diarrheal episode during a 10-month period. c jejuni was isolated from 437 (14%) of 3,038 outpatients with diarrhea. these patients had no distinct clinical presentation and were more likely to have a mixed infection than were patients infected with othe ...	1983	6886491
[infections caused by campylobacter jejuni/coli].		1983	6848351
prevalence of campylobacter jejuni in chicken wings.	campylobacter jejuni was found in 82.9% of 94 chicken wing packages analyzed on the day of arrival at supermarkets and in 15.5% of 45 packages obtained from the supermarket shelves a few days later. the number of bacterial cells ranged from 10(2) to 10(3.9) per wing. the prevalence of c. jejuni in the wings varied with the brand, the day of sampling, and the age of the product.	1983	6847181
an outbreak of campylobacter enteritis--a rheumatological followup survey.	one hundred and thirty individuals with bacteriologically proven campylobacter jejuni enteric infection in a single outbreak were studied. eighty-eight patients had gastrointestinal symptoms. one probable example of reactive arthritis was found. this is a frequency similar to that found in other comparable series of salmonella typhimurium and shigella flexneri infections, suggesting a common pathogenesis for the reactive arthritis associated with these 3 infections.	1983	6842468
occurrence of plasmid dna in serologically defined strains of campylobacter jejuni and campylobacter coli.	forty campylobacter jejuni and 17 campylobacter coli strains that constitute the set of reference strains for our serotyping scheme were each examined for the presence of plasmid dna. agarose gel electrophoresis of alkaline-extracted dna showed the occurrence of 29 bands in 11 c. jejuni strains and 40 bands in c. coli strains. plasmids ranged in size from 1.6 to 70 megadaltons. most strains that carried plasmids had between 2 and 6 of them; however, one strain had 14 plasmids, and two strains co ...	1983	6840847
prevalence of campylobacter jejuni in two california chicken processing plants.	two federally inspected california chicken processing plants participated in campylobacter jejuni prevalence studies. twelve sampling sites were included in each of four groups. groups were based on bird age, scald water temperature, and plant sampled. scald water temperatures of 60 degrees c (140 degrees f) did not contribute to a lower prevalence of c. jejuni in edible parts, as did temperatures of 53 degrees c (127 degrees f) and 49 degrees c (120 degrees f). the feather picker and chilling t ...	1983	6830212
house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni.	a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ...	1983	6830213
experimental campylobacter jejuni infection of adult mice.	ha-icr adult mice were studied to develop an animal model for campylobacter jejuni enteritis in humans. fecal and ileal cultures made by selective and nonselective methods showed that c. jejuni and related organisms are not bowel commensals. intragastric feeding of 10(8) cfu of three different strains of c. jejuni produced infection in 100% of the animals, and infection rates were dose dependent. pretreatment with antibiotics or opiates was not necessary to induce infection. fresh isolates and s ...	1983	6832823
campylobacter jejuni-associated hemolytic-uremic syndrome in a mother and daughter.	a mother and daughter with campylobacter jejuni-associated hemolytic-uremic syndrome (hus) are discussed. the mother was hospitalized with bloody diarrhea and hus; c jejuni was isolated from her stool. the 2-year-old daughter had been admitted five days prior to her mother with hus following a three-day prodrome of vomiting and diarrhea. multiple stool cultures were negative for enteric pathogens; however, cultures were not obtained until the eighth hospital day and after antibiotic therapy. ext ...	1983	6823430
the serotype and biotype distribution of clinical isolates of campylobacter jejuni and campylobacter coli over a three-year period.	two hundred eighty-five isolates of campylobacter jejuni-campylobacter coli from children with gastroenteritis at the hospital for sick children (toronto, canada) over a three-year period were biotyped by the hippurate hydrolysis test and serotyped on the basis of thermostable, soluble antigens by the passive hemagglutination technique. hippurate-negative strains (c. coli) were only 3.2% of the isolates. ninety-seven percent of the isolates were serotypable with 55 antisera. about half of the st ...	1983	6827141
campylobacter hyointestinalis (new species) isolated from swine with lesions of proliferative ileitis.	intestines from 48 swine with enteric disease were examined by bacteriologic cultural technique for the presence of various campylobacter species. histopathologic techniques were used to determine whether the submitted specimens had lesions of either swine proliferative ileitis or other enteric diseases. three species of campylobacter were identified as campylobacter jejuni/coli, campylobacter sputorum ss mucosalis, and campylobacter hyointestinalis (proposed new species) on the basis of biochem ...	1983	6838031
isolation of plasmids encoding tetracycline resistance from campylobacter jejuni strains isolated from simians.	fifteen isolates of tetracycline-resistant campylobacter jejuni were recovered from stool samples of cynomologous monkeys (macaca fascicularis) housed at the university of washington primate research center, seattle. resistance was associated with carriage of a 38-megadalton plasmid which was transmissible to other strains of c. jejuni but not to escherichia coli. seven isolates also contained a 2.6-megadalton plasmid which was phenotypically cryptic.	1983	6838189
serum antibodies in campylobacter enteritis.	a solid-phase enzyme immunoassay method for the measurement of total and class-specific serum antibodies to campylobacter jejuni was developed. the test was found to be both sensitive and specific. immunoglobulin m, g, and a antibodies were detected in about 90% of sera collected from patients recovering from campylobacter enteritis, with the pattern of appearance and decline of these antibodies conforming to that usually seen in an acute infection. a poor antibody response was noted in patients ...	1983	6885981
symmetrical reactive oligoarthritis after campylobacter jejuni enteritis--case report and study of the synovial complement.	a 34-year-old man, in whom sacroiliitis had been diagnosed 5 years previously, presented in july 1982 with reactive arthritis following campylobacter jejuni enteritis. diarrhoea was stopped by erythromycin but joint effusion recurred. in order to clarify the relationship between campylobacter jejuni and the immunological system, we proceeded with a study of the synovial complement. the results were compared with those obtained in some other arthropathies.	1983	6880409
campylobacter jejuni as a bacterial cause of diarrhoea in ile-ife, nigeria.	a total of 495 diarrhoea and non-diarrhoea patients whose ages ranged between 5 and 39 years were examined for the presence of campylobacter jejuni, salmonella and shigella species. about 12% of the specimens from diarrhoea patients were positive for campylobacter jejuni compared with 6% and 10% for salmonella and shigella species. in contrast 2%, 0% and 1% of the samples from non-diarrhoea patients were positive for campylobacter jejuni, salmonella and shigella species respectively. most (62%) ...	1983	6886413
experience with the microbiologic diagnosis of campylobacter enteritis in an office laboratory.	campylobacter jejuni has been recognized as a frequent cause of bacterial diarrhea in infants and children. c. jejuni is a fastidious, gram-negative, comma-shaped or sea gull-shaped, curved rod which is capable, particularly during the summer months, of causing slimy mucoid, blood diarrhea, abdominal pain and fever. in our pediatric office laboratory we found over a 12-month period that 14 (10%) of 126 stool specimens contained this pathogen. all but two children were diagnosed during the late s ...	1983	6889157
serologic study of two clusters of infection due to campylobacter jejuni.	the serologic responses to campylobacter jejuni in persons involved in two clusters of infection and in control subjects were studied. in the first cluster, in which previously unexposed persons drank raw milk, the attack rate was high and elevated complement-fixing (cf) and specific igg and igm antibodies were demonstrated. in the second cluster, involving farmers who chronically drank raw milk, the attack rate was low, but titers of cf and igg antibodies were high in both affected and unaffect ...	1983	6842019
attempts to isolate campylobacter jejuni from various body sites.	clinical material collected from various body sites, but excluding faeces, was cultured on either selective or non-selective media for campylobacter spp. campylobacter jejuni was found in only two (0.18%) of 1100 specimens; both positive specimens were urine. from these results it is suggested that c jejuni is an uncommon finding in clinical material other than faeces.	1983	6853732
identification of campylobacter jejuni in macaca fascicularis imported from indonesia.	campylobacter jejuni was selectively cultured in 33 (66%) of 50 macaca fascicularis that had been imported from indonesia. as there was no published information on the incidence of campylobacter infection in nonhuman primates from indonesia, a survey was conducted to determine the presence and incidence of campylobacter jejuni in 50 macaques before they were exported from indonesia. the organism was positively identified in 18 (36%) of the specimens examined. repeat cultures after importation an ...	1983	6855191
incidence of plasmid dna in strains of campylobacter jejuni isolated from stool specimens at 37 c and 43 c.		1983	6842034
comparison of selective media for isolation of campylobacter jejuni/coli.	a comparison of skirrow's, butzler's, blaser's, campy-bap and preston media for campylobacter spp was made using human, animal and environmental specimens. butzler's medium gave the lowest isolation rate and preston medium, which was the most selective, the highest isolation rate. enrichment culture using preston enrichment broth gave a higher isolation rate than direct plating onto preston medium.	1983	6822680
campylobacter enteritis in the united states. a multicenter study.	during a 15-month study, 8097 fecal specimens submitted to clinical microbiology laboratories at eight hospitals in different parts of the united states were examined. campylobacter jejuni was isolated from 4.6%, salmonella from 2.3%, and shigella from 1.0%. isolation rates for each pathogen were highest from stool specimens that were watery, bloody, or contained leukocytes. the peak isolation rate for c. jejuni was in persons ages 10 to 29 years; for salmonella, in children younger than age 10 ...	1983	6830079
culture supernatants of campylobacter jejuni induce a secretory response in jejunal segments of adult rats.	culture supernatants of four campylobacter jejuni strains induced a net sodium secretory flux (plasma-lumen) and an impaired glucose transport in perfused jejunal segments of adult rats in vivo.	1983	6832835
[contribution to the epidemiology of campylobacter jejuni. from asymptomatic excretion by a cow in the cowshed to overt disease in over 500 persons].	an outbreak of c. jejuni enteritis involving participants of a jogging rally is described. information was obtained by questionnaire about food consumption, incubation period, duration of illness and symptomatology. a drink prepared with raw milk was incriminated as vehicle of the outbreak. more than 500 runners were symptomatic with an attack rate of over 75%. secondary cases were rare. using serotyping, milk from a single cow fecally excreting c. jejuni type 2 was suspected to be the outbreak ...	1983	6836250
[campylobacter fetus bacteremia].	five cases of campylobacter fetus bacteremia are reported. this germ, found in blood cultures, induces high fever and is accompanied by either gastroenteritis with colitis or thrombophlebitis. other, but much rarer septic sites are the meninges and endocardium. patients already debilitated by chronic disease are more susceptible to bacteremia with campylobacter fetus, which worsens the prognosis of the infection. although non-debilitated patients can contract this infection, the prognosis here i ...	1983	6836251
serotypes of campylobacter jejuni and campylobacter coli in bacteremic, hospitalized children.		1983	6833802
selective enrichment broth medium for isolation of campylobacter jejuni.	a new selective campylobacter enrichment broth for the isolation of campylobacter jejuni has been tested with fecal specimens of human, poultry, and bovine origin. we compared the isolation rate with that from the simultaneous direct plating of a duplicate specimen. of the 380 duplicate specimens examined comparatively, we obtained a 46.3% increase in isolation of c. jejuni by using the new campylobacter enrichment broth medium.	1983	6863504
human campylobacter infection associated with certified raw milk.	between may 27 and june 18, 1981, 50 individuals in 30 households in suburban atlanta, georgia, had a gastrointestinal illness caused by campylobacter jejuni. epidemiologic evidence strongly associated consumption of unpasteurized milk with illness. a culture survey confirmed fecal carriage of c. jejuni by cows in the implicated dairy and in a control dairy, but failed to document presence of the organism in the milk. the standard plate counts and leukocyte counts (two indicators of microbiologi ...	1983	6837559
identification and characterization of campylobacter jejuni outer membrane proteins.	outer membrane proteins from isolates of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125i-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. most isolates contained a single major band with ...	1983	6618667
association of campylobacter jejuni with enteritis in dogs and cats.	campylobacter jejuni was recovered from 59 of 505 (11.7 per cent) dogs with diarrhoea as compared with only two of 122 (1.6 per cent) dogs without diarrhoea. however, there was no significant difference between campylobacter isolations from 142 cats with and without diarrhoea. c jejuni infections were commonly associated with chronic diarrhoea in both species and appropriate therapy abolished clinical signs and excretion of the organism in faeces in most cases. c jejuni may be responsible for so ...	1983	6649362
[serologic demonstration of antibodies against campylobacter jejuni/coli in man].	antibody response to campylobacter jejuni/coli infections in man was studied by microagglutination assay against homologues organisms isolated from patients and by complement fixation test against a commercially available group-specific antigen of c. jejuni/coli. titers of agglutinating antibodies raise within 1-2 weeks post infectionem (p.i.) to an extent of about 320 (reciprocal titer) and persist about 3-4 months. cf-antibody titers raise only to about 40 but they persist for a long time. bec ...	1983	6680399
[occurrence of campylobacter fetus subsp. jejuni in mice and guinea pigs from experimental animal establishments].		1983	6624307
campylobacter jejuni in children with diarrhea in mexico city.	the frequency of campylobacter jejuni infection in children under 4 years of age, with and without diarrhea, attending the hospital infantil de mexico was studied. campylobacter was cultured from rectal swabs in 23 (8.7%) of 265 children with diarrhea, with the highest isolation rate in the 7- to 12-month age group, and in 2 (4%) of 54 children without diarrhea. from 5 (22%) of the children infected with campylobacter, salmonella or shigella sp. were simultaneously cultured. major clinical featu ...	1983	6835851
effects of disinfectants on campylobacter jejuni.	because of the increasing recognition that campylobacter jejuni is an important enteric pathogen of humans, we studied the effects of widely used disinfectants on the viability of this organism. at an inoculum size of 10(3) to 10(4) cfu/ml, 1.25 mg of hypochlorite per liter killed three strains within 1 min. at an inoculum size of 10(6) to 10(7) cfu/ml, 5 mg of hypochlorite per liter killed three strains within 15 min. killing of similar concentrations of c. jejuni and escherichia coli by hypoch ...	1983	6305266
aetiological studies on hospital in-patients with acute diarrhoea in calcutta.	bacterial enteropathogens and rotavirus were sought in 356 cases with acute diarrhoea admitted to the infectious diseases hospital, calcutta. one or more pathogens were isolated from 74.7% of the cases. single enteropathogens could be detected from 66% and multiple enteropathogens from 8.7% of the patients. vibrio cholerae biotype el tor, rotavirus, v. parahaemolyticus, and enteropathogenic and enterotoxigenic escherichia coli were the major pathogens detected. rotavirus was detected from 7.6% o ...	1983	6306873
quality assurance of selective culture media for bacteria, moulds and yeasts: an attempt at standardization at the international level.	to facilitate monitoring of culture media, a simple quantitative streaking technique, implying ever-decreasing numbers of colony-forming units per surface area, as in spiral plating, was developed. the procedure evaluates, in quantitative terms, the ability of media (1) to support the formation of colonies by organisms that it was designed to grow and (2) to resist colonization by organisms that it is expected to suppress. the procedure was therefore termed ecometric evaluation. the ecometric re ...	1983	6348012
value of stool examination in patients with diarrhoea.	findings of stool examinations in 1593 patients with diarrhoea due to a single enteric pathogen--enterotoxigenic escherichia coli rotavirus, shigella, campylobacter jejuni, vibrio cholerae 0:1, entamoeba histolytica, or giardia lamblia--were reviewed to determine how well they predicted the agent associated with the diarrhoea. specimens were examined visually for blood and mucus, tested for ph, and examined under a microscope for the presence of red and white blood cells, parasites, and stool fa ...	1983	6307460
detection of campylobacter jejuni and other potential pathogens in travellers' diarrhoea in bangladesh.	to determine the distribution of pathogens associated with travellers' diarrhoea in bangladesh, we have studied 269 expatriates who presented with diarrhoea to our clinic, over a one-year period. patients were interviewed about their history of diarrhoea and a stool specimen examined for parasites and cultured for campylobacter jejuni, shigella sp., salmonella sp., vibrio and aeromonas hydrophila and plesiomonas shigelloides. c. jejuni and shigella sp. were each isolated from 45 patients (17%) a ...	1983	6314488
treatment of acute nonspecific gastroenteritis of infants and young children with erythromycin.	a double-blind placebo-controlled trial of erythromycin ethylsuccinate was conducted in 65 infants and young children hospitalized with acute nonspecific gastroenteritis. etiologic agents included rotaviruses (29%), campylobacter jejuni (17%), "classical" enteropathogenic escherichia coli (12%), enterotoxigenic e. coli (11%), salmonella (9%), shigella (2%), and giardia lamblia (2%). no pathogens were obtained from 25 (38%) children. treatment with erythromycin had no effect on the course of the ...	1983	6349401
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces?	the role of enrichment culture for the isolation of campylobacter jejuni from faeces is discussed. it is concluded that enrichment culture is only necessary for those specimens where it is anticipated that the number of organisms is likely to be low. in a trial of a blood free enrichment broth (ccd broth) and the modified preston enrichment broth the latter gave significantly superior results.	1983	6361073
[incidence of campylobacter jejuni/coli from healthy people in yamaguchi, japan].		1983	6406618
[campylobacter infection in children].	enteritis by campylobacter jejuni/coli is by far the most frequent clinical entity of campylobacter-infection. it may be compared to salmonella-enteritis as for its epidemiologic importance. the course of the disease may be dramatic, but can be successfully treated as soon as the pathogen is known. in prematures and newborn babies infections with campylobacter fetus ssp. fetus are life-threatening and early diagnosis is of life-saving importance. five observations of campylobacter-enteritis in c ...	1983	6352463
enteropathogen carriage by healthy individuals living in an area with poor sanitation.	faecal carriage of bacterial enteropathogens (enteropathogenic escherichia coli (epec), shigellae and salmonellae) was studied in 265 individuals: 65 infants 3-6 months of age (50 bottle-fed and 15 breast-fed), 100 school-age children 8-10 years of age and 100 adults 21-50 years of age. all were apparently healthy, did not have gastrointestinal symptoms, had not received antibiotics in the preceding fortnight and were not malnourished. enteropathogens were isolated from the faeces of 24 individu ...	1983	6363528
antibodies against an acid extract from a single campylobacter strain in hospitalized campylobacter patients.	an acid glycine extract from a single campylobacter jejuni strain was used as the antigen in the enzyme immunoassay (eia). elevated values indicating the presence of specific igm and/or igg antibodies were found in 73% of 52 patients hospitalized for campylobacter infection. a further 6% of the patients who had lower eia values in paired serum samples showed at least a 20% change in eia values. the same sera had been studied earlier for agglutinating antibodies using four reference campylobacter ...	1983	6352507
isolation of campylobacter jejuni from an appendix.	campylobacter jejuni skirrow biotype 1, lior serotype 8 was isolated from the appendix of an 11-year-old boy who had a 6-h history of acute abdominal pain. histological diagnosis on the appendix section was early acute appendicitis. dilute carbol fuchsin stain and indirect fluorescent antibody test performed on the appendix section also revealed the presence of campylobacter sp. the patient developed a significant bactericidal antibody titer of 1,024, providing substantial clinical evidence of t ...	1983	6352736
[survey of mass incidences of diarrhea (15 cases) due to campylobacter jejuni in tokyo from 1979 to 1981].		1983	6418837
[203 cases of campylobacter jejuni enteritis].		1983	6421957
[isolation of campylobacter jejuni from chicken feces and their survival period in minced chicken].		1983	6421960
hydrogenase activity in catalase-positive strains of campylobacter spp.	a rapid hydrogenase assay has been developed which may be useful in separating the species campylobacter jejuni and c. coli from the subspecies c. fetus subsp. fetus and c. fetus subsp. venerealis. this assay employs the impermeant redox dye benzyl viologen, and positive determinations can be made within 20 min. all strains of c. jejuni and c. coli were found to be strongly hydrogenase positive. all strains of c. fetus subsp. fetus and c. fetus subsp. venerealis were negative for hydrogenase whe ...	1983	6355162
survival of campylobacter jejuni in different media and faeces at different temperatures and times of preservation.	nineteen campylobacter jejuni strains survived best in suspensions with approximately 10(9) bacteria in 4% albumin solution at-60 degrees c for 17 months (18 out of 19). at the same temperature and during the same period they survived less well in milk (11 out of 19). recovery of campylobacters after 7 weeks was abundant in albumin, and somewhat less in milk. at -20 degrees c survival in milk was worse; 17 out of 19 cultures did not survive 5 weeks in milk and 7 died in albumin. at 4 degrees c a ...	1983	6362314
treatment of campylobacter-associated enteritis with erythromycin.	twenty-six infants and young children with acute dehydrating diarrhea associated with campylobacter jejuni participated in a randomized, double-blind, placebo-controlled therapeutic trial. of 25 patients who completed the study, 11 were treated for five days with oral erythromycin ethylsuccinate (40 mg/kg/day in divided doses), and the rest received matched placebo. although erythromycin significantly shortened the duration of c jejuni excretion, it appeared to exert no effect on the clinical co ...	1983	6337473
avian wildlife reservoir of campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. in norway.	cloacal swabs from 540 wild-living birds were cultured for campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. the carrier rates detected were as follows: c. fetus subsp. jejuni, 28.4%; yersinia spp., 1.2%; and salmonella spp., 0.8%. all birds were apparently healthy when captured. c. fetus subsp. jejuni was isolated from 11 of the 40 bird species examined. among birds inhabiting the city of oslo, the highest isolation rate was found in crows (corvus corone cornix) (89.8%), fol ...	1983	6338824
[infection due to campylobacter jejuni].		1983	6347653
a longitudinal study of the prevalence of bacterial enteric pathogens among adults with diarrhea in bangkok, thailand.	six hundred sixty adults with diarrhea treated at bamrasnaradura hospital, bangkok, thailand were investigated to determine the prevalence, seasonality, and severity of diarrhea associated with bacterial enteric pathogens in 1980 and 1981. shigella were isolated from 27% and vibrio parahaemolyticus from 19% of the patients studied. enterotoxigenic escherichia coli (5%), salmonella (3%), non-01 vibrio cholerae (3%), campylobacter jejuni (1%), and group f vibrio (less than 1%) were isolated from a ...	1983	6370564
a new selective medium for the isolation of campylobacter jejuni from human faeces.	a new selective medium, butzler's medium virion, for the isolation of campylobacter jejuni from human faeces is described. this medium contains the following antibiotics per liter: cefoperazone 15 mg, rifampicin 10 mg, colistin 10,000 iu, and amphotericin b 2 mg. at 42 degrees c there was no difference in the isolation rate on butzler's medium virion and butzler's medium oxoid, but the competing faecal flora was best suppressed by the new medium which allows easier reading of plates and better r ...	1983	6354716
in vitro antibacterial activity of norfloxacin (mk-0366, am-715) and other agents against gastrointestinal tract pathogens.	a comparison was made of the in vitro activities of norfloxacin and of nine other orally administered antibacterial agents against 180 clinical isolates representing the bacterial species most frequently implicated in infections of the gastrointestinal tract in humans. the 90% minimal inhibitory concentrations showed norfloxacin to be 4, 15, 4, 17, 17, 17, and 33 times more active than the next best compound tested against campylobacter fetus subsp. jejuni, escherichia coli, salmonella spp., shi ...	1983	6219622
in vitro activity of ci-919 (at-2266), an oral antipseudomonal compound.	we tested ci-919 (at-2266), a nalidixic acid analog, against 555 gram-positive and gram-negative bacteria, using microbroth or agar dilution methods. the activity of ci-919 was compared with those of cephalosporins, tobramycin, ticarcillin, dicloxacillin, rifampin, chloramphenicol, ampicillin, and trimethoprimsulfamethoxazole. the minimal inhibitory concentrations of ci-919 for 90% of isolates were (in micrograms per milliliter): pseudomonas spp. (including pseudomonas aeruginosa), 4.0; enteroba ...	1983	6223577
[comparative bacteriological studies in diarrheal diseases with special reference to campylobacter jejuni].		1983	6356643
[epidemiology of campylobacter jejuni in youth camps].		1983	6224112
correlation of an expanded direct fluorescent-antibody system with an established passive hemagglutination system for serogrouping strains of campylobacter jejuni and campylobacter coli.	rabbits were inoculated with whole, formalinized cells from eight passive hemagglutination reference strains of campylobacter. fluorescein isothiocyanate-labeled immunoglobulin g from these antisera defined seven new direct fluorescent-antibody serogroups of c. jejuni and one new serogroup of c. coli. this expanded the campylobacter direct fluorescent antibody system to include 17 serogroups of c. jejuni, 3 serogroups of c. coli, and 2 serogroups of c. fetus. we then compared the passive hemaggl ...	1983	6358249
nucleic acids in the classification of campylobacters.	the importance of campylobacters in human disease has stimulated improvements in the methods for identification of strains from hospitals and the environment. reliable and accurate identification depends on a sound classification for which nucleic acid analyses provide fundamental information about species relationships. studies on the genus campylobacter show that the genome dna of species have base compositions of 29 to 38 mol% g + c and molecular weights of 1.54 x 10(9) to 2.31 x 10(9). campy ...	1983	6194994
cholera-like enterotoxin produced by campylobacter jejuni. characterisation and clinical significance.	the presence and clinical significance of enterotoxins produced by campylobacter jejuni were investigated. the supernatant of a prototype virulent strain grown in supplemented medium induced intraluminal fluid secretion in rat ileal loop but not in rabbit ileal loop or the infant mouse assay. it induced elongation and increased intracellular cyclic amp levels in chinese hamster ovary cells. toxin activity was blocked by cholera antitoxin and was destroyed by heat and high or low ph; its molecula ...	1983	6135079
swedish isolates of campylobacter jejuni/coli do not produce cytotonic or cytotoxic enterotoxins.		1983	6137720
the polymicrobial origin of intestinal infections in homosexual men.	to determine the microbial cause and the clinical and pathologic correlates of anorectal and intestinal symptoms in homosexually active men, we performed comprehensive microbiologic studies, anoscopy, sigmoid-oscopy, and rectal biopsy in men examined in a clinic for sexually transmitted diseases. enteric pathogens were found in 95 of 119 consecutive homosexual men with anorectal or intestinal symptoms and in 29 of 75 randomly selected homosexual men without such symptoms (p less than 0.001). the ...	1983	6308444
application of serotyping and chromosomal restriction endonuclease digest analysis in investigating a laboratory-acquired case of campylobacter jejuni enteritis.	a frequently passaged laboratory strain of campylobacter jejuni was confirmed by serotyping on the basis of thermostable antigens and by bacterial chromosomal restriction endonuclease digests to be the causative agent of enteritis in a laboratory worker.	1983	6317712
characterization of tetracycline resistance plasmids from campylobacter jejuni and campylobacter coli.	tetracycline resistance in strains of campylobacter jejuni and campylobacter coli was mediated by plasmids. intra- and interspecies transfer was demonstrated within the genus campylobacter. buoyant densities of plasmid dnas ranged from 1.691 to 1.694 g/cm3 (31 to 33% guanine plus cytosine). restriction enzymes acci, bcli, bglii, and psti were found to be most useful for comparing the plasmids. the molecular weight of c. jejuni plasmid pmak175 was 44.7 kilobases (29 x 10(6), and the other plasmid ...	1983	6318666
enteropathogenic agents in children with diarrhoea in rural zaire.	a systematic study of enteropathogenic agents in the stools of children was carried out in a rural area of kivu province in zaire in june, 1979. 84 inpatients and 271 outpatients with diarrhoea were investigated together with 117 inpatient and 203 outpatient controls without diarrhoea. campylobacter jejuni was the most frequently recovered pathogen in both inpatients (24%) and outpatients (13.7%). enterotoxigenic escherichia coli was the next most common among children presenting as outpatients ...	1983	6131221