Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| methylation-dependent dna discrimination in natural transformation of campylobacter jejuni. | campylobacter jejuni, a leading cause of bacterial gastroenteritis, is naturally competent. like many competent organisms, c. jejuni restricts the dna that can be used for transformation to minimize undesirable changes in the chromosome. although c. jejuni can be transformed by c. jejuni-derived dna, it is poorly transformed by the same dna propagated in escherichia coli or produced with pcr. our work indicates that methylation plays an important role in marking dna for transformation. we have i ... | 2017 | 28855338 |
| cleaning and disinfection programs against campylobacter jejuni for broiler chickens: productive performance, microbiological assessment and characterization. | detailed cleaning and disinfection programs aims to reduce infection pressure from microorganisms from one flock to the next. however, studies evaluating the benefits to poultry performance, the sanitary status of the facilities, and the sanitary quality of the meat are rarely found. thus, this study was designed to evaluate 2 cleaning and disinfecting programs regarding their influence on productive performance, elimination of campylobacter, and characterization of campylobacter jejuni strains ... | 2017 | 28854757 |
| prevalence and risk factors for thermotolerant species of campylobacter in poultry meat at retail in europe. | the thermotolerant species campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis are the causative agents of the human illness called campylobacteriosis. this infection represents a threat for the health of consumers in europe. it is well known that poultry meat is an important food vehicle of campylobacter infection. as emerged from the reported scientific literature published between 2006 and 2016, poultry meat sold at retail level in europe represents an i ... | 2017 | 28854745 |
| isolation and identification of campylobacter spp. from poultry and poultry by-products in tunisia by conventional culture method and multiplex real-time pcr. | thermophilic campylobacter spp. are one of the primary causes of bacterial human diarrhea. the consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. the aims of this study were to determine the prevalence of thermophilic campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm campylobacter jejuni and campylobacter coli isolates by using the multiplex pcr assay. two hundred fifty fre ... | 2017 | 28853632 |
| genome reduction for niche association in campylobacter hepaticus, a cause of spotty liver disease in poultry. | the term "spotty liver disease" (sld) has been used since the late 1990s for a condition seen in the uk and australia that primarily affects free range laying hens around peak lay, causing acute mortality and a fall in egg production. a novel thermophilic sld-associated campylobacter was reported in the united kingdom (uk) in 2015. subsequently, similar isolates occurring in australia were formally described as a new species, campylobacter hepaticus. we describe the comparative genomics of 10 c. ... | 2017 | 28848714 |
| campylobacter jejuni vertebral osteomyelitis. | 2017 | 28845786 | |
| evaluation of primitive ground water supplies as a risk factor for the development of major waterborne zoonosis in egyptian children living in rural areas. | endemic waterborne zoonosis frequently occurs in both developed and less developed countries. thus, bio-surveillance of waterborne zoonosis is a "necessity" for the implementation of effective preventive public health measures in egyptian rural areas. the primitive individual water supplies created by the rural agriculture population, primarily from ground water, usually maximize the customers' exposure to impurity pathogens via diffused humans and animal excreta or wastages. the current study a ... | 2017 | 28843417 |
| pet-associated campylobacteriosis: a persisting public health concern. | campylobacter is regarded as a leading cause of human bacterial gastroenteritis in the united states. we report on a case of laboratory-confirmed campylobacter jejuni infection in the commonwealth of pennsylvania among members of a household living with a laboratory-confirmed but non-speciated campylobacter-infected puppy. we describe an outbreak of likely dog-associated campylobacteriosis, the risk factors, potential routes of exposure and the clinical features in the exposed family members, wh ... | 2017 | 28834267 |
| fecal shedding of campylobacter jejuni and campylobacter coli among feral pigs in texas. | the population and range of feral pigs in the united states are rapidly expanding, yet key knowledge gaps exist regarding their role in the ecology and transmission of foodborne pathogens. our objectives were to estimate the prevalence of campylobacter jejuni and campylobacter coli shedding among feral pigs throughout texas and to identify risk factors for positive status. faecal samples were collected from feral pigs in texas from february 2014 through may 2015, and target organisms were detect ... | 2017 | 28834190 |
| application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products. | foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ... | 2017 | 28825446 |
| total synthesis of β-d-ido-heptopyranosides related to capsular polysaccharides of campylobacter jejuni hs:4. | the 6-deoxy-β-d-ido-heptopyranoside related to the capsular polysaccharides of c. jejuni hs:4 is very remarkable, owing to the unique, multifaceted structural features that have been combined into one molecule, which include (1) the rare ido-configuration, (2) the unusual 7-carbon backbone, and (3) the challenging β-(1→2)-cis-anomeric configuration. two distinct strategies toward the total synthesis of this interesting target are reported. the first involved establishment of the β-d-idopyranosyl ... | 2017 | 28809484 |
| the influence of the gut microbiota composition on campylobacter jejuni colonization in chickens. | campylobacter jejuni (c. jejuni)-host-interaction may be affected by the host's gut microbiota through competitive exclusion, metabolites or modification of the immune response. to understand this interaction c. jejuni colonization and local immune responses were compared in chickens with different gut microbiota composition. birds were treated with an antibiotic cocktail (at) (experiment 1 and 2) or raised under germ-free (gf) conditions (experiment 3). at 18 days post hatch (dph), they were ei ... | 2017 | 28808158 |
| essential genome of campylobacter jejuni. | campylobacter species are a leading cause of bacterial foodborne illness worldwide. despite the global efforts to curb them, campylobacter infections have increased continuously in both developed and developing countries. the development of effective strategies to control the infection by this pathogen is warranted. the essential genes of bacteria are the most prominent targets for this purpose. in this study, we used transposon sequencing (tn-seq) of a genome-saturating library of tn5 insertion ... | 2017 | 28806924 |
| transplanted human fecal microbiota enhanced guillain barré syndrome autoantibody responses after campylobacter jejuni infection in c57bl/6 mice. | campylobacter jejuni is the leading antecedent infection to the autoimmune neuropathy guillain-barré syndrome (gbs), which is accompanied by an autoimmune anti-ganglioside antibody attack on peripheral nerves. previously, we showed that contrasting immune responses mediate c. jejuni induced colitis and autoimmunity in interleukin-10 (il-10)-deficient mice, dependent upon the infecting strain. strains from colitis patients elicited t helper 1 (th1)-dependent inflammatory responses while strains f ... | 2017 | 28789710 |
| cftr reduces microtubule-dependent campylobacter jejuni invasion. | campylobacter jejuni (c. jejuni) is gastroenteritis inducible food-born pathogen. invasion and adhesion process are essential for leading gastroenteritis in c. jejuni infection process. as against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. cystic fibrosis transmembrane conductance regulator (cftr) play the main role in waterfulux in intestin ... | 2017 | 28784926 |
| animal-related factors associated with moderate-to-severe diarrhea in children younger than five years in western kenya: a matched case-control study. | diarrheal disease remains among the leading causes of global mortality in children younger than 5 years. exposure to domestic animals may be a risk factor for diarrheal disease. the objectives of this study were to identify animal-related exposures associated with cases of moderate-to-severe diarrhea (msd) in children in rural western kenya, and to identify the major zoonotic enteric pathogens present in domestic animals residing in the homesteads of case and control children. | 2017 | 28783751 |
| whole-genome sequencing of campylobacter jejuni isolated from danish routine human stool samples reveals surprising degree of clustering. | outbreaks of campylobacter are traditionally considered to be rare, however rather than being the true nature of the disease, this may reflect our present inability to detect them. the aim of this study was to determine the genetic and epidemiological degree of clustering among campylobacter jejuni isolates from danish patients. | 2017 | 28782648 |
| the gene for the s7 ribosomal protein of chlamydia trachomatis: characterization within the chlamydial sfr operon. | the prokaryotic ribosomal operon, str, contains open reading frames for the two elongation factors, elongation factor g (ef-g) and elongation factor tu (ef-tu), and ribosomal proteins 57 and s12. the dna sequence and predicted amino acid sequence for s7 from chlamydia trachomatis are presented and compared with homologues from other prokaryotes. also, the relationship of the s7 gene to the open reading frames for ribosomal protein s12 and ef-g is described. significant amino acid homology is als ... | 1992 | 28776799 |
| intrinsic and extrinsic aspects on campylobacter jejuni biofilms. | biofilm represents a way of life that allows greater survival of microorganisms in hostile habitats. campylobacter jejuni is able to form biofilms in vitro and on surfaces at several points in the poultry production chain. genetic determinants related to their formation are expressed differently between strains and external conditions are decisive in this respect. our approach combines phylogenetic analysis and the presence of seven specific genes linked to biofilm formation in association with ... | 2017 | 28769900 |
| campylobacter jejuni as a cause of diarrhoea. | this study was carried out to isolate and identify campylobacter jejuni from stool samples of patients suffering from diarrhoea. the study spanned a two-year period and c. jejuni was isolated in 10 (4.5%) out of 220 patients of diarrhoea] disorders. in samples of stool collected from 50 healthy individuals, c. jejuni was isolated in one (2%) case. further, to isolate other enteropathogens alongwith c. jejuni, procedures were undertaken simultaneously to identify salmonella, shigella and vibrio c ... | 1997 | 28769509 |
| lactobacillus fermentum 3872 as a potential tool for combatting campylobacter jejuni infections. | due to the global spread of multidrug resistant pathogenic bacteria, alternative approaches in combating infectious diseases are required. one such approach is the use of probiotics. lactobacillus fermentum 3872 is a promising probiotic bacterium producing a range of antimicrobial compounds, such as hydrogen peroxide and lactic acid. in addition, previous studies involving genome sequencing and analysis of l. fermentum 3872 allowed the identification of a gene encoding a cell surface protein ref ... | 2017 | 28766992 |
| campylobacter jejuni impairs sodium transport and epithelial barrier function via cytokine release in human colon. | campylobacter jejuni is the most prevalent cause of foodborne bacterial enteritis worldwide. patients present with diarrhea and immune responses lead to complications like arthritis and irritable bowel syndrome. although studies exist in animal and cell models, we aimed at a functional and structural characterization of intestinal dysfunction and the involved regulatory mechanisms in human colon. first, in patients' colonic biopsies, sodium malabsorption was identified as an important diarrheal ... | 2017 | 28766554 |
| structures and transport dynamics of a campylobacter jejuni multidrug efflux pump. | resistance-nodulation-cell division efflux pumps are integral membrane proteins that catalyze the export of substrates across cell membranes. within the hydrophobe-amphiphile efflux subfamily, these resistance-nodulation-cell division proteins largely form trimeric efflux pumps. the drug efflux process has been proposed to entail a synchronized motion between subunits of the trimer to advance the transport cycle, leading to the extrusion of drug molecules. here we use x-ray crystallography and s ... | 2017 | 28761097 |
| molecular dynamics simulations of quinolone resistance-associated t86i and p104s mutations in campylobacter jejuni gyra: unraveling structural repercussions. | gyra is a clinically validated therapeutic drug target that catalyzes changes in dna topology by introducing negative supercoils into dna. the broad-spectrum quinolones are hitherto the most potent antibacterial agents targeted toward gyra. increasing resistance to quinolones is accompanied by several mutations in the quinolone resistance-determining region of gyra. in the present study, we report a comprehensive picture of the dynamic behavior of wild-type, t86i, and t86i/p104s mutants (mts) of ... | 2017 | 28759328 |
| guillain-barré syndrome complicating dengue fever: two case reports. | guillain-barré syndrome is a rare neurological manifestation associated with dengue infection. more common antecedent infections include campylobacter jejuni and cytomegalovirus infection. here, we report two cases of guillain-barré syndrome complicating dengue infection. | 2017 | 28757747 |
| population structure and attribution of human clinical campylobacter jejuni isolates from central europe to livestock and environmental sources. | campylobacter jejuni is among the most prevalent causes of human bacterial gastroenteritis worldwide. domesticated animals and, especially, chicken meat are considered to be the main sources of infections. however, the contribution of surface waters and wildlife in c. jejuni transmission to humans is not well understood. we have evaluated the source attribution potential of a six-gene multiplex pcr (mpcr) method coupled with structure analysis on a set of 410 c. jejuni strains isolated from envi ... | 2017 | 28755449 |
| absence of nucleotide-oligomerization-domain-2 is associated with less distinct disease in campylobacter jejuni infected secondary abiotic il-10 deficient mice. | human campylobacter jejuni-infections are progressively increasing worldwide. despite their high prevalence and socioeconomic impact the underlying mechanisms of pathogen-host-interactions are only incompletely understood. given that the innate immune receptor nucleotide-oligomerization-domain-2 (nod2) is involved in clearance of enteropathogens, we here evaluated its role in murine campylobacteriosis. to address this, we applied nod2-deficient il-10(-/-) (nod2(-/-) il-10(-/-)) mice and il-10(-/ ... | 2017 | 28752081 |
| complete genome sequence of campylobacter jejuni subsp. jejuni atcc 35925. | here, we report the complete genome sequence of campylobacter jejuni atcc 35925, an avian isolate from sweden. the genome gives insight into the atcc 35925 strain's remarkable ability to tolerate copper and its permissiveness to plasmid transformation. | 2017 | 28751407 |
| acute pancreatitis caused by campylobacter jejuni in a child with cystic fibrosis. | 2017 | 28751082 | |
| probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca. | the probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca, were evaluated. this strain was selected for study from the isolated lactobacillus strains because it has specific anti-microbial activity against campylobacter jejuni atcc 33560, camp. jejuni nctc 11168, listeria monocytogenes atcc 7644, and bacillus subtilis atcc 8633. lact. curvatus dn317 showed an auto-aggregation percentage of 72% and presented the highest co-aggregation with lact. monocytoge ... | 2017 | 28741151 |
| synthesis and antimicrobial activity of chiral quaternary n-spiro ammonium bromides with 3',4'-dihydro-1'h-spiro[isoindoline-2,2'-isoquinoline] skeleton. | a new class of highly functionalized tetrahydroisoquinolines with a quaternary carbon stereocenter was synthesized starting from an easily accessible l-tartaric acid. nine strains of bacteria (staphylococcus aureus, streptococcus pyogenes, streptococcus mutans, streptococcus salivarius, bacillus subtilis, enterococcus faecalis, moraxella catarrhalis, escherichia coli, campylobacter jejuni) were used for the determination of minimal inhibitory concentration (mic) and minimal bactericidal concentr ... | 2017 | 28740363 |
| in vitro characteristics of lactobacillus spp. strains isolated from the chicken digestive tract and their role in the inhibition of campylobacter colonization. | campylobacter jejuni/coli infections are the leading cause of bacterial diarrheal illnesses in humans. many epidemiological studies indicate that improperly prepared meat from chickens that carry a high load of campylobacter in their intestinal tracts is the key source of human infections. lab, mainly members of the lactococcus and lactobacillus genera, increasingly have been tested as vehicles for the delivery of heterologous bacterial or viral antigens to animal mucosal immune systems. thus, t ... | 2017 | 28736979 |
| cardamom, cumin, and dill weed essential oils: chemical compositions, antimicrobial activities, and mechanisms of action against campylobacter spp. | natural antimicrobials as well as essential oils (eos) have gained interest to inhibit pathogenic microorganisms and to control food borne diseases. campylobacter spp. are one of the most common causative agents of gastroenteritis. in this study, cardamom, cumin, and dill weed eos were evaluated for their antibacterial activities against campylobacter jejuni and campylobacter coli by using agar-well diffusion and broth microdilution methods, along with the mechanisms of antimicrobial action. che ... | 2017 | 28714890 |
| a genome-wide association study to identify diagnostic markers for human pathogenic campylobacter jejuni strains. | campylobacter jejuni is a leading human enteric pathogen worldwide and despite an improved understanding of its biology, ecology, and epidemiology, limited tools exist for identifying strains that are likely to cause disease. in the current study, we used subtyping data in a database representing over 24,000 isolates collected through various surveillance projects in canada to identify 166 representative genomes from prevalent c. jejuni subtypes for whole genome sequencing. the sequence data was ... | 2017 | 28713351 |
| genetic diversity of thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. | the objective of this study was to investigate a clonal relationship among thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. a total of 128 thermotolerant campylobacter spp. (89 c. jejuni and 39 c. coli) isolates from six poultry meat chains were examined. these isolates were from: a) hens from breeder flocks, b) chickens on the farm (at ages 1 wk and 5 wk), c) chicken carcasses in the slaughterhouse, and d) chicken carcasses in the r ... | 2017 | 28712509 |
| grape pomace improves performance, antioxidant status, fecal microbiota and meat quality of piglets. | in the present study, grape pomace (gp) was used as feed additive in the diet of weaned piglets in order to develop innovative feedstuffs and to investigate their potential beneficial effects on welfare, productivity and meat quality. for examining the antioxidant capacity of the experimental feeds, 24 piglets of 20 days old were assigned to two experimental groups receiving standard or experimental diet for 30 days. blood and tissues collections were performed at four different time-points, 2, ... | 2017 | 28712376 |
| development of an isothermal amplification-based assay for the rapid visual detection of salmonella bacteria. | the efficient and timely detection of pathogens is a major concern worldwide. the aim of this study was to establish a rapid detection method for salmonella bacteria in food samples to facilitate timely treatment. widely used detection methods currently include culture-based methods and pcr-based methods. the former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. in this study, a sensiti ... | 2017 | 28711269 |
| the molecular mechanisms of ciprofloxacin resistance in clinical campylobacter jejuni and their genotyping characteristics in beijing, china. | we assessed the susceptibility of 182 campylobacter jejuni isolates from patients with diarrhea to eight antibiotics and analyzed the molecular mechanisms of ciprofloxacin resistance as well as the genetic characteristics based on multilocus sequence typing (mlst). the c257t mutation was found on the quinolone resistance-determining region (qrdr) of the gyra gene in all ciprofloxacin-resistant strains. mutations on the qrdr of the gyrb gene were silent. a total of 74 strains had 7 inverted repea ... | 2017 | 28696791 |
| from the gut to the heart: campylobacter jejuni enteritis leading to myopericarditis. | campylobacter jejuni enteritis is the most common enteric infection in the developed world. although rare, pericarditis and myopericarditis have been increasingly documented as complications following campylobacteriosis. the present paper implicates that myopericarditis and non-ischemic cardiomyopathy following campylobacter jejuni infection might be more prevalent than initially thought and perhaps underreported so far. therefore, it is imperative to perform the appropriate initial diagnostic t ... | 2017 | 28690959 |
| small intestinal pro-inflammatory immune responses following campylobacter jejuni infection of secondary abiotic il-10(-/-) mice lacking nucleotide-oligomerization-domain-2. | host immune responses are crucial for combating enteropathogenic infections including campylobacter jejuni. within 1 week following peroral c. jejuni infection, secondary abiotic il-10(-/-) mice develop severe immunopathological sequelae affecting the colon (ulcerative enterocolitis). in the present study, we addressed whether pathogen-induced pro-inflammatory immune responses could also be observed in the small intestines dependent on the innate receptor nucleotide-oligomerization-domain-protei ... | 2017 | 28690881 |
| β-resorcylic acid, a phytophenolic compound, reduces campylobacter jejuni in postharvest poultry. | human campylobacter infections, a leading foodborne illness globally, has been linked with the high prevalence of this bacterium on raw retail chicken products. reduction of campylobacter counts on poultry products would greatly reduce the risk of subsequent infections in humans. to this end, this study investigated the potential of the phytophenolic compound β-resorcylic acid (br) to reduce campylobacter counts on postharvest poultry (chicken skin or meat). four trials in total, two each on thi ... | 2017 | 28686495 |
| role of eptc in biofilm formation by campylobacter jejuni nctc11168 on polystyrene and glass surfaces. | the complex roles of cell surface modification in the biofilm formation of campylobacter jejuni, a major cause of worldwide foodborne diarrheal disease, are poorly understood. in a screen of mutants from random transposon mutagenesis, an insertional mutation in eptc gene (cj0256) resulted in a significant decrease in c. jejuni nctc11168 biofilm formation (<20%) on major food contact surfaces, such as polystyrene and borosilicate glass, compared with wild-type cells (p < 0.05). in c. jejuni strai ... | 2017 | 28683522 |
| speciation trajectories in recombining bacterial species. | it is generally agreed that bacterial diversity can be classified into genetically and ecologically cohesive units, but what produces such variation is a topic of intensive research. recombination may maintain coherent species of frequently recombining bacteria, but the emergence of distinct clusters within a recombining species, and the impact of habitat structure in this process are not well described, limiting our understanding of how new species are created. here we present a model of bacter ... | 2017 | 28671999 |
| a study on campylobacter jejuni and campylobacter coli through commercial broiler production chains in thailand: antimicrobial resistance, the characterization of dna gyrase subunit a mutation, and genetic diversity by flagellin a gene restriction fragment length polymorphism. | contaminated poultry meat is regarded as the main source of human campylobacteriosis. during september 2014 and february 2015, breeder flocks, hatcheries, and broiler farms from two chicken production chains were investigated chronologically. five commercial breeder flocks (breeder flocks 1-5), two hatcheries (hatcheries a and b), and five broiler flocks (broiler flocks 1-5) were sampled in this study. campylobacter colonization of both breeder and broiler flocks was determined from cloacal swab ... | 2017 | 28665716 |
| campylobacter fetus impairs barrier function in ht-29/b6 cells through focal tight junction alterations and leaks. | infections by campylobacter species are the most common foodborne zoonotic disease worldwide. campylobacter jejuni and c. coli are isolated most frequently from human stool samples, but severe infections by c. fetus (cf), which can cause gastroenteritis, septicemia, and abortion, are also found. this study aims at the characterization of pathological changes in cf infection using an intestinal epithelial cell model. the cf-induced epithelial barrier defects appeared earlier than those of avian c ... | 2017 | 28662272 |
| importance of campylobacter jejuni flis and fliw in flagella biogenesis and flagellin secretion. | flagella-driven motility enables bacteria to reach their favorable niche within the host. the human foodborne pathogen campylobacter jejuni produces two heavily glycosylated structural flagellins (flaa and flab) that form the flagellar filament. it also encodes the non-structural flac flagellin which is secreted through the flagellum and has been implicated in host cell invasion. the mechanisms that regulate c. jejuni flagellin biogenesis and guide the proteins to the export apparatus are differ ... | 2017 | 28659885 |
| effects of dry whey powder and calcium butyrate supplementation of corn/soybean-based diets on productive performance, duodenal histological integrity, and campylobacter colonization in broilers. | campylobacter is the main cause of gastroenteritis in humans in industrialized countries, and poultry is its principal reservoir and source of human infections. dietary supplementation of broiler feed with additives could improve productive performance and elicit health benefits that might reduce campylobacter contamination during primary production. the aim of this study was to assess the effect of dietary supplementation with whey (a prebiotic) and calcium butyrate (a salt of a short-chain fat ... | 2017 | 28651615 |
| campylobacter species isolated from pigs in grenada exhibited novel clones: genotypes and antimicrobial resistance profiles of sequence types. | infections caused by campylobacter species pose a severe threat to public health worldwide. however, in grenada, the occurrence and characteristics of campylobacter in food animals, including pigs, remain mostly unknown. in this study, we identified the sequence types (sts) of campylobacter from young healthy pigs in grenada and compared the results with previous studies in grenada and other countries. antimicrobial resistance patterns and diversity of the campylobacter clones were evaluated. ni ... | 2017 | 28650672 |
| discovery of a glutamine kinase required for the biosynthesis of the o-methyl phosphoramidate modifications found in the capsular polysaccharides of campylobacter jejuni. | bacterial capsular polysaccharides (cps) are complex carbohydrate structures that play a role in the overall fitness of the organism. campylobacter jejuni, known for being a major cause of bacterial gastroenteritis worldwide, produces a cps with a unique o-methyl phosphoramidate (meopn) modification on specific sugar residues. the formation of p-n bonds in nature is relatively rare, and the pathway for the assembly of the phosphoramidate moiety in the cps of c. jejuni is unknown. in this investi ... | 2017 | 28650156 |
| impact of built-up-litter and commercial antimicrobials on salmonella and campylobacter contamination of broiler carcasses processed at a pilot mobile poultry-processing unit. | the small-scale mobile poultry-processing unit (mppu) produced raw poultry products are of particular food safety concern due to exemption of usda poultry products inspection act. limited studies reported the microbial quality and safety of mppu-processed poultry carcasses. this study evaluated the salmonella and campylobacter prevalence in broiler ceca and on mppu-processed carcasses and efficacy of commercial antimicrobials against campylobacter jejuni on broilers. in study i, straight-run hub ... | 2017 | 28649571 |
| hydroxylamine-induced oxidation of ferrous carbonylated truncated hemoglobins from mycobacterium tuberculosis and campylobacter jejuni is limited by carbon monoxide dissociation. | hydroxylamine (ha) is an oxidant of ferrous globins and its action has been reported to be inhibited by co, even though this mechanism has not been clarified. here, kinetics of the ha-mediated oxidation of ferrous carbonylated mycobacterium tuberculosis truncated hemoglobin n and o (mt-trhbn(ii)-co and mt-trhbo(ii)-co, respectively) and campylobacter jejuni truncated hemoglobin p (cj-trhbp(ii)-co), at ph 7.2 and 20.0 °c, are reported. mixing mt-trhbn(ii)-co, mt-trhbo(ii)-co, and cj-trhbp(ii)-co ... | 2017 | 28646425 |
| cytolethal distending toxin enhances radiosensitivity in prostate cancer cells by regulating autophagy. | cytolethal distending toxin (cdt) produced by campylobacter jejuni contains three subunits: cdta, cdtb, and cdtc. among these three toxin subunits, cdtb is the toxic moiety of cdt with dnase i activity, resulting in dna double-strand breaks (dsb) and, consequently, cell cycle arrest at the g2/m stage and apoptosis. radiation therapy is an effective modality for the treatment of localized prostate cancer (pca). however, patients often develop radioresistance. owing to its particular biochemical p ... | 2017 | 28642840 |
| recurrent campylobacter jejuni bacteremia in a patient with hypogammaglobulinemia: a case report. | although some cases of recurrent bacteremia due to campylobacter jejuni have been reported in immunocompromised patients, antibiotic treatment strategies to eradicate c. jejuni and prevent recurrent infections in immunocompromised patients have not been established. authors' experience of such rare cases should be shared for improving patients' outcomes. | 2017 | 28640123 |
| biochemical characterization of wbkc, an n-formyltransferase from brucella melitensis. | it has become increasingly apparent within the last several years that unusual n-formylated sugars are often found on the o-antigens of such gram negative pathogenic organisms as francisella tularensis, campylobacter jejuni, and providencia alcalifaciens, among others. indeed, in some species of brucella, for example, the o-antigen contains 1,2-linked 4-formamido-4,6-dideoxy-α-d-mannosyl groups. these sugars, often referred to as n-formylperosamine, are synthesized in pathways initiating with gd ... | 2017 | 28636341 |
| campylobacter jejuni myocarditis: a journey from the gut to the heart. | campylobacter jejuni is an unusual cause of myocarditis and could easily be missed. | 2017 | 28634541 |
| draft genome sequence of campylobacter jejuni cam970 and c. coli cam962, associated with a large outbreak of foodborne illness in fukuoka, japan, in 2016. | here, we report the draft genome sequences of campylobacter jejuni cam970 and c. coli cam962, which were associated with a large outbreak of foodborne illness originating from undercooked chicken sushi in fukuoka, japan, in may 2016. their genome sizes were 1,690,901 and 1,704,736 bp, with 22 and 23 rrnas, 9 and 9 trnas, and 411× and 419× coverage for c. jejuni cam970 and c. coli cam962, respectively. | 2017 | 28619796 |
| isolation and characterization of a non-specific lipid transfer protein from chelidonium majus l. latex. | plant non-specific lipid transfer proteins (nsltps) are small basic proteins, which mostly play a role in intracellular lipid transport and antimicrobial defense. recently it was shown using shotgun proteomic approach that the whole plant extract of chelidonium majus l. (papaveraceae) contains relatively abundant nsltps. therefore the aim of the work was to isolate and characterize nsltp from c. majus latex. results obtained using pcr approach with degenerate primers showed the presence of nsltp ... | 2017 | 28619636 |
| mlst genotypes of campylobacter jejuni isolated from broiler products, dairy cattle and human campylobacteriosis cases in lithuania. | campylobacter (c.) jejuni is the leading cause of human campylobacteriosis worldwide. we performed a molecular epidemiological study to investigate the genetic relationship among c. jejuni strains isolated from human diarrhoeal patients, broiler products and dairy cattle in lithuania. | 2017 | 28619013 |
| differential survival of hyper-aerotolerant campylobacter jejuni under different gas conditions. | campylobacter jejuni accounts for a significant number of foodborne illnesses around the world. c. jejuni is microaerophilic and typically does not survive efficiently in oxygen-rich conditions. we recently reported that hyper-aerotolerant (hat) c. jejuni are highly prevalent in retail poultry meat. to assess the capabilities of hat c. jejuni in foodborne transmission and infection, in this study, we investigated the prevalence of virulence genes in hat c. jejuni and the survival in poultry meat ... | 2017 | 28611753 |
| rnaseq reveals complex response of campylobacter jejuni to ovine bile and in vivo gallbladder environment. | colonization of the gallbladder by enteric pathogens such as salmonella typhi, listeria monocytogenes, and campylobacter jejuni is thought to play a key role in transmission and persistence of these important zoonotic agents; however, little is known about the molecular mechanisms that allow for bacterial survival within this harsh environment. recently, a highly virulent c. jejuni sheep abortion (sa) clone represented by the clinical isolate ia3902 has emerged as the dominant cause for sheep ab ... | 2017 | 28611744 |
| campylobacter jejuni bacteremia in a liver cirrhosis patient and review of literature: a case study. | campylobacter infection causes gastrointestinal symptoms such as abdominal pain or diarrhea. occasionally, campylobacter bacteremia affects immunocompromised patients; however, serious outcomes are known to be rare. here, we present a case of a patient with campylobacter bacteremia who had underlying liver cirrhosis. the patient had fever and diarrhea. these symptoms subsided after treatment with cefotaxime. campylobacter jejuni was isolated in the blood culture after 10 days. in addition, previ ... | 2017 | 28608661 |
| application of long amplicon propidium monoazide-pcr to assess the effects of temperature and background microbiota on pathogens in river water. | the decay rates of enteric waterborne pathogens were evaluated following the introduction of yersinia enterocolitica, salmonella enterica, campylobacter jejuni and arcobacter butzleri into river water at different temperatures (5, 15 and 25°c) for a period of 28 days. to improve the accuracy of the results a molecular viability assay, long amplicon propidium monoazide-polymerase chain reaction (pma-pcr), was used to quantify the viable cell concentration and results from pcr with and without pma ... | 2017 | 28598346 |
| phage exposure causes dynamic shifts in the expression states of specific phase-variable genes of campylobacter jejuni. | phase variation (pv) creates phenotypic heterogeneity at high frequencies and in a reversible manner. this phenomenon allows bacteria to adapt to a variety of different environments and selective pressures. in campylobacterjejuni this reversible adaptive process is mediated by mutations in homopolymeric g/c tracts. many c. jejuni-specific phages are dependent on phase-variable surface structures for successful infection. we previously identified the capsular polysaccharide (cps) moiety, meopn-ga ... | 2017 | 28597819 |
| towards diagnostic metagenomics of campylobacter in fecal samples. | the development of diagnostic metagenomics is driven by the need for universal, culture-independent methods for detection and characterization of pathogens to substitute the time-consuming, organism-specific, and often culture-based laboratory procedures for epidemiological source-tracing. some of the challenges in diagnostic metagenomics are, that it requires a great next-generation sequencing depth and unautomated data analysis. | 2017 | 28595575 |
| immune responses upon campylobacter jejuni infection of secondary abiotic mice lacking nucleotide-oligomerization-domain-2. | campylobacter jejuni infections are of rising importance worldwide. given that innate immune receptors including nucleotide-oligomerization-domain-2 (nod2) are essentially involved in combating enteropathogenic infections, we here surveyed the impact of nod2 in murine campylobacteriosis. | 2017 | 28592996 |
| generation of the membrane potential and its impact on the motility, atp production and growth in campylobacter jejuni. | the generation of a membrane potential (δψ), the major constituent of the proton motive force (pmf), is crucial for atp synthesis, transport of nutrients and flagellar rotation. campylobacter jejuni harbors a branched electron transport chain, enabling respiration with different electron donors and acceptors. here, we demonstrate that a relatively high δψ is only generated in the presence of either formate as electron donor or oxygen as electron acceptor, in combination with an acceptor/donor re ... | 2017 | 28586527 |
| detection and prevalence of antimicrobial resistance genes in <i>campylobacter</i> spp. isolated from chickens and humans. | campylobacter spp. are common pathogenic bacteria in both veterinary and human medicine. infections caused by campylobacter spp. are usually treated using antibiotics. however, the injudicious use of antibiotics has been proven to spearhead the emergence of antibiotic resistance. the purpose of this study was to detect the prevalence of antibiotic resistance genes in campylobacter spp. isolated from chickens and human clinical cases in south africa. one hundred and sixty one isolates of campylob ... | 2017 | 28582978 |
| next generation sequencing-based multigene panel for high throughput detection of food-borne pathogens. | contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. bacteria that cause foodborne diseases are known as foodborne pathogens (fbps). accurate detection methods that are able to reveal the presence of fbps in food matrices are in constant demand, in order to ensure safe foods wi ... | 2017 | 28578266 |
| confirmed identification and toxin profiling of campylobacter jejuni using a thermostabilized multiplex pcr formulation. | cytolethal distending toxin (cdt) producing campylobacter jejuni species are one of the leading causes of human gastroenteritis worldwide. the main intent of the study was to develop a multiplex pcr assay for the confirmed identification and toxin profiling of c. jejuni. the genes targeted were rpo b as genus specific, hip o for species; cdt a, cdt b, cdt c encoding respective subunit proteins of cdt with internal amplification control (iac). to enhance its application as a pre-mixed ready-to-us ... | 2017 | 28574617 |
| pathogen-induced ubiquitin-editing enzyme a20 bifunctionally shuts off nf-κb and caspase-8-dependent apoptotic cell death. | the human pathogen helicobacter pylori infects more than half of the world's population and is a paradigm for persistent yet asymptomatic infection but increases the risk for chronic gastritis and gastric adenocarcinoma. for successful colonization, h. pylori needs to subvert the host cell death response, which serves to confine pathogen infection by killing infected cells and preventing malignant transformation. infection of gastric epithelial cells by h. pylori provokes direct and fast activat ... | 2017 | 28574503 |
| profiling of campylobacter jejuni proteome in exponential and stationary phase of growth. | campylobacter jejuni has been reported as a major cause of bacterial food-borne enteritides in developed countries during the last decade. despite its fastidious growth requirements, including low level of oxygen and high level of co2, this pathogen is able to persist in the environment without permanent loss of its viability and virulence. as c. jejuni is not able to multiply outside a host, the cells spend significant amount of time in stationary phase of growth. the entry into the stationary ... | 2017 | 28572800 |
| the effect of microbial challenge on the intestinal proteome of broiler chickens. | in poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. uncovering the localised molecular mechanisms that occur during the early and important periods of growth that allow birds to grow optimally is important for this species. the exposure of young chicks to used litter from older flocks, containing mixed microbial populations, is a widely utilised model in poultry research. it rarely causes mortality but effects an immunogenic stimu ... | 2016 | 28572745 |
| sourcer: classification and source attribution of infectious agents among heterogeneous populations. | zoonotic diseases are a major cause of morbidity, and productivity losses in both human and animal populations. identifying the source of food-borne zoonoses (e.g. an animal reservoir or food product) is crucial for the identification and prioritisation of food safety interventions. for many zoonotic diseases it is difficult to attribute human cases to sources of infection because there is little epidemiological information on the cases. however, microbial strain typing allows zoonotic pathogens ... | 2017 | 28558033 |
| whole-genome sequence of the bacteriophage-sensitive strain campylobacter jejuni nctc12662. | campylobacter jejuni nctc12662 has been the choice bacteriophage isolation strain due to its susceptibility to c. jejuni bacteriophages. this trait makes it a good candidate for studying bacteriophage-host interactions. we report here the whole-genome sequence of nctc12662, allowing future elucidation of the molecular mechanisms of phage-host interactions in c. jejuni. | 2017 | 28546493 |
| metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen campylobacter jejuni. | campylobacter jejuni is one of the leading infectious causes of food-borne illness around the world. its ability to persistently colonize the intestinal tract of a broad range of hosts, including food-producing animals, is central to its epidemiology since most infections are due to the consumption of contaminated food products. using a highly saturated transposon insertion library combined with next-generation sequencing and a mouse model of infection, we have carried out a comprehensive genome ... | 2017 | 28542173 |
| comparing the genetic diversity and antimicrobial resistance profiles of campylobacter jejuni recovered from cattle and humans. | campylobacter jejuni, a leading cause of gastroenteritis in humans, is a foodborne pathogen that can reside in chickens, pigs, and cattle. because resistance to fluoroquinolones and macrolides, which are commonly used to treat human infections, has emerged in c. jejuni, it is imperative to continously monitor resistance patterns and examine the genetic variation in strains from human infections and animal reservoirs. our previous study of c. jejuni from human campylobacteriosis cases showed a si ... | 2017 | 28536568 |
| dissemination of erm(b) and its associated multidrug-resistance genomic islands in campylobacter from 2013 to 2015. | a total of 1372 campylobacter isolates (1107 campylobacter coli and 265 campylobacter jejuni) were obtained from 3462 samples collected from slaughterhouses and farms in three representative regions of china (shandong, guangdong, and shanghai) over three successive years (2013-2015). of these, 84 (84/1372, 6.1%) were erm(b)-positive, and all 84 positive isolates were identified as c. coli (83 chicken isolates and one swine isolate). the prevalence of erm(b) in campylobacter isolates was compared ... | 2017 | 28532801 |
| a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay. | salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ... | 2017 | 28526589 |
| association of anti-gangliosides antibodies and anti-cmv antibodies in guillain-barré syndrome. | numerous types of infection were closely related to gbs, mainly including campylobacter jejuni, cytomegalovirus, which may lead to the production of anti-gangliosides antibodies (aga). currently, although there are increased studies on the aga and a few studies of anti-cmv antibodies in gbs, the association between them remains poorly documented. therefore, our research aims to analyze the correlation of anti-cmv antibodies and aga in gbs. | 2017 | 28523231 |
| lactobacillus johnsonii ameliorates intestinal, extra-intestinal and systemic pro-inflammatory immune responses following murine campylobacter jejuni infection. | campylobacter jejuni infections are progressively increasing worldwide. probiotic treatment might open novel therapeutic or even prophylactic approaches to combat campylobacteriosis. in the present study secondary abiotic mice were generated by broad-spectrum antibiotic treatment and perorally reassociated with a commensal murine lactobacillus johnsonii strain either 14 days before (i.e. prophylactic regimen) or 7 days after (i.e. therapeutic regimen) peroral c. jejuni strain 81-176 infection. f ... | 2017 | 28522817 |
| immunoprevalence to six waterborne pathogens in beachgoers at boquerón beach, puerto rico: application of a microsphere-based salivary antibody multiplex immunoassay. | waterborne infectious diseases are a major public health concern worldwide. few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. in this article, we applied a multiplex bead-based immunoassay capable of ... | 2017 | 28507984 |
| development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens. | bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ... | 2017 | 28505584 |
| prevalence and characterization of campylobacter jejuni isolated from retail chicken in tianjin, china. | campylobacter jejuni is an important foodborne pathogen worldwide; however, there is a lack of information on the prevalence and antibiotic-resistant profile of c. jejuni in the people's republic of china. we determined the prevalence and characteristics of c. jejuni on the retail level in tianjin, one of the five national central cities in china. a total of 227 samples of chicken wings, legs, and breasts were collected from supermarkets and wet markets; 42 of these samples were confirmed to be ... | 2017 | 28504616 |
| prevalence of campylobacter species in wild birds of south korea. | campylobacter species cause human gastrointestinal infections worldwide. they commonly inhabit intestines of avian species including wild birds. they might play a role in the spread of infections to humans and other bird species. the prevalence of campylobacter species in 2164 faecal samples of wild birds (representing 71 species and 28 families) captured across the korean peninsula was evaluated in this study. the overall prevalence was 15.3% (332/2164). bird species belonging to the family cha ... | 2017 | 28503965 |
| novel sampling method for assessing human-pathogen interactions in the natural environment using boot socks and citizen scientists, with application to campylobacter seasonality. | this paper introduces a novel method for sampling pathogens in natural environments. it uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). this sampling method is implemented using a citizen science approach: groups of three walkers wearing boot socks undertook one of six routes, 40 times over 16 months in the north west ... | 2017 | 28500040 |
| identification of potential campylobacter jejuni genes involved in biofilm formation by ez-tn5 transposome mutagenesis. | biofilm formation has been suggested to play a role in the survival of campylobacter jejuni in the environment and contribute to the high incidence of human campylobacteriosis. molecular studies of biofilm formation by campylobacter are sparse. | 2017 | 28499399 |
| comparative proteomics reveals differences in host-pathogen interaction between infectious and commensal relationship with campylobacter jejuni. | campylobacter jejuni is the leading food-borne poisoning in industrialized countries. while the bacteria causes disease in humans, it merely colonizes the gut in poultry or pigs, where seems to establish a commensal relationship. until now, few studies have been conducted to elucidate the relationship between c. jejuni and its different hosts. in this work, a comparative proteomics approach was used to identify the underlying mechanisms involved in the divergent outcome following c. jejuni infec ... | 2017 | 28491823 |
| cj0440c affects flagella formation and in vivo colonization of erythromycin-susceptible and -resistant campylobacter jejuni. | campylobacter jejuni is one of the most common foodborne pathogen worldwide. a putative transcriptional regulator, cj0440c, was up-regulated in the erythromycin-resistant c. jejuni, however, the precise role of cj0440c is yet to be determined. the aim of this study was to determine the biological functions of cj0440c. the cj0440c isogenic mutants were constructed from erythromycin-susceptible c. jejuni nctc 11168 (s) and -resistant c. jejuni 68-er (r), designating as sm and rm, respectively. the ... | 2017 | 28487689 |
| trans-cinnamaldehyde, carvacrol, and eugenol reduce campylobacter jejuni colonization factors and expression of virulence genes in vitro. | campylobacter jejuni is a major foodborne pathogen that causes severe gastroenteritis in humans characterized by fever, diarrhea, and abdominal cramps. in the human gut, campylobacter adheres and invades the intestinal epithelium followed by cytolethal distending toxin mediated cell death, and enteritis. reducing the attachment and invasion of campylobacter to intestinal epithelium and expression of its virulence factors such as motility and cytolethal distending toxin (cdt) production could pot ... | 2017 | 28487683 |
| microbiota-derived short-chain fatty acids modulate expression of campylobacter jejuni determinants required for commensalism and virulence. | campylobacter jejuni promotes commensalism in the intestinal tracts of avian hosts and diarrheal disease in humans, yet components of intestinal environments recognized as spatial cues specific for different intestinal regions by the bacterium to initiate interactions in either host are mostly unknown. by analyzing a c. jejuni acetogenesis mutant defective in converting acetyl coenzyme a (ac-coa) to acetate and commensal colonization of young chicks, we discovered evidence for in vivo microbiota ... | 2017 | 28487428 |
| identification and differentiation of campylobacter species by high-resolution melting curve analysis. | campylobacter jejuni and campylobacter coli are the important food-born zoonotic pathogen, also are leading causes of human food borne illnesses worldwide. cadf gene is expressed in all c. jejuni and c. coli strains and mediates cell binding to the cell matrix protein, fibronectin. high-resolution melting (hrm) analysis is emerging as an efficient and robust method for discriminating dna sequence variants. the goal of this study was to apply hrm analysis to identification of c. jejuni and c. col ... | 2017 | 28478199 |
| epidemiology of antimicrobial resistant campylobacter spp. isolated from retail meats in canada. | campylobacter is an important zoonotic pathogen found in livestock and can cause illness in humans following consumption of raw and undercooked meat products. the objectives of this study were to determine the prevalence of campylobacter spp. in retail meat (poultry, turkey, pork and beef) purchased in alberta, canada and to assess antimicrobial resistance and genetic relatedness of recovered campylobacter strains with previously isolated strains from clinical and environmental sources. a compar ... | 2017 | 28477522 |
| causality assessment of serious neurologic adverse events following bopv national vaccination campaign in israel. | during 2013-2014 israel experienced a continuous circulation of wild poliovirus type 1 (wpv1) but with no clinical cases. wpv1 circulation was gradually terminated following a national vaccination campaign of bivalent oral poliovirus vaccine (bopv) for 943,587 children < 10 years. four cases of children with neurological manifestations that appeared following bopv vaccinations were reported during the campaign: three of guillain-barré syndrome (gbs) and one of acute disseminated encephalomyeliti ... | 2016 | 28471617 |
| generation of recombinant n-linked glycoproteins in e. coli. | the production of n-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, escherichia coli. this methods chapter will outline the components and procedures needed to produce n-linked glycoproteins in e. coli, utilizing campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. to ensure a successful outcome, various methods will be highlighted that c ... | 2017 | 28470609 |
| isolation of campylobacter species from stool samples by use of a filtration method: assessment from a united states-based population. | fecal samples submitted to our clinical microbiology laboratory from patients in the philadelphia region were prospectively analyzed for campylobacter species other than c. jejuni and c. coli using a filtration method and microaerobic conditions with increased h2 concentrations. of 225 samples tested, 13 (5.8%) yielded campylobacter species, with frequent isolation of c. concisus the majority of campylobacter species were not clinically significant. additional studies in u.s. populations are war ... | 2017 | 28468859 |
| phase-variable changes in the position of o-methyl phosphoramidate modifications on the polysaccharide capsule of campylobacter jejuni modulate serum resistance. | campylobacter jejuni polysaccharide capsules (cps) are characterized by the presence of nonstoichiometric o-methyl phosphoramidate (meopn) modifications. the lack of stoichiometry is due to phase variation at homopolymeric tracts within the meopn transferase genes. c. jejuni strain 81-176 contains two meopn transferase genes and has been shown previously to contain meopn modifications at the 2 and 6 positions of the galactose (gal) moiety in the cps. we demonstrate here that one of the two meopn ... | 2017 | 28461446 |
| genome-wide fitness analyses of the foodborne pathogen campylobacter jejuni in in vitro and in vivo models. | campylobacter is the most common cause of foodborne bacterial illness worldwide. faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. there is a lack of insight into the mechanisms driving c. jejuni growth and survival within hosts and the environment. here, we report a detailed analysis of c. jejuni fitness across models reflecting stages in its life cycle. transposon (tn) gene-inactivation libraries were generated in three c. jej ... | 2017 | 28455506 |
| core genome multilocus sequence typing scheme for stable, comparative analyses of campylobacter jejuni and c. coli human disease isolates. | human campylobacteriosis, caused by campylobacter jejuni and c. coli, remains a leading cause of bacterial gastroenteritis in many countries, but the epidemiology of campylobacteriosis outbreaks remains poorly defined, largely due to limitations in the resolution and comparability of isolate characterization methods. whole-genome sequencing (wgs) data enable the improvement of sequence-based typing approaches, such as multilocus sequence typing (mlst), by substantially increasing the number of l ... | 2017 | 28446571 |
| nonspecific effects of oral polio vaccine on diarrheal burden and etiology among bangladeshi infants. | as the global polio eradication initiative prepares to cease use of oral polio vaccine (opv) in 2020, there is increasing interest in understanding if oral vaccination provides non-specific immunity to other infections so that the consequences of this transition can be effectively planned for and mitigated. | 2017 | 28444240 |
| simultaneous identification of campylobacter jejuni, campylobacter coli, and campylobacter lari with smartcycler-based multiplex quantitative polymerase chain reaction. | consumption of campylobacter contaminated food or water is a leading cause of human acute gastroenteritis. campylobacter jejuni, campylobacter coli, and campylobacter lari account for over 95% of total campylobacter infections. a multiplex quantitative polymerase chain reaction (qpcr) for simultaneous identification of c. jejuni, c. coli, and c. lari was developed for use with the smartcycler ii system. | 2017 | 28440688 |