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outbreaks of waterborne infectious intestinal disease in england and wales, 1992-2003.we reviewed the epidemiological and microbiological characteristics of 89 reported outbreaks of waterborne infectious intestinal disease affecting 4321 people in england and wales over the period 1992-2003. public water supplies were implicated in 24 outbreaks (27%), private water supplies in 25 (28%), swimming pools in 35 (39%) and other sources in five outbreaks (6%). cryptosporidium was implicated in 69% of outbreaks, campylobacter sp. in 14%, giardia in 2%, e. coli o157 in 3% and astrovirus ...200616690002
a new antibiotic combination for frozen bovine semen 1. control of mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus.systematic evaluations of new combinations of antibiotics for the control of bovine mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus in a bovine frozen semen process were made. these organisms were standardized to 10(5) to 10(6) colony forming unit (cfu) and inoculated into each ml of raw semen. antibiotics in a final volume of 0.02 ml were added to each ml of the raw semen and were contained at the same concentration in the nonglycerol portion of the extend ...198816726381
the use of loglinear model to evaluate factors associated with sero-prevalence of campylobacter fetus in dairy cattle.campylobacter fetus is one of the leading infections causing bovine infertility and abortion. in a cross-sectional study, the associations of parity, herd, serological status against leptospira hardjo and haemophilus somnus with the sero-prevalence of campylobacter fetus in dairy cattle were evaluated. four hundred dairy cows from three herds were included in the study. blood samples from the cows were collected in december 1986. an enzyme-linked immunosorbant assay (elisa) was used to determine ...199016726898
reproductive performance of beef heifers: effects of vulvo-vaginitis, ureaplasma diversum and prebreeding antibiotic administration.a group of 450 heifers, 13 to 15 mo of age, were individually identified, vaccinated (ibr, pi(3), leptospira, campylobacter fetus), examined (body condition score, reproductive tract evaluation, assessment of vaginal lesions), and cultured for u. diversum . heifers were randomly allocated to either a treated group given chlortetracycline (approximately 350 mg/hd/d for 30 d in a grain crumble) or a nontreated control group. prebreeding, most heifers showed signs of vulvovaginitis, 44% cultured po ...199316727333
temporal prevalence of antimicrobial resistance in campylobacter spp. from beef cattle in alberta feedlots.antimicrobial resistance (amr) was temporally assessed in campylobacters isolated from beef cattle (7,738 fecal samples from 2,622 animals) in four commercial feedlots in alberta. all calves were administered chlortetracycline and oxytetracycline in feed, and a majority of the animals (93%) were injected with long-acting oxytetracycline upon arrival at the feedlot. fecal samples from individual animals were collected upon arrival (i.e., entry sample), 69 days (standard deviation [sd] = 3 days) a ...200616751519
identification of campylobacter fetus subspecies by phenotypic differentiation and pcr.the species campylobacter fetus is divided into the subspecies c. fetus subsp. venerealis and c. fetus subsp. fetus, which differ in their epidemiologies and clinical importance. the differences between these subspecies make accurate distinction between the two essential. first, the value of seven key tests for the traditional differentiation of c. fetus was investigated. afterwards, the results of the phenotypic differentiation and pcr were compared to address the question of the reliability of ...200616757592
phase variation and genomic architecture changes in azospirillum.the plant growth-promoting rhizobacterium azospirillum lipoferum 4b generates in vitro at high frequency a stable nonswimming phase variant designated 4v(i), which is distinguishable from the wild type by the differential absorption of dyes. the frequency of variants generated by a reca mutant of a. lipoferum 4b was increased up to 10-fold. the pleiotropic modifications characteristic of the phase variant are well documented, but the molecular processes involved are unknown. here, the objective ...200616855225
detection sensitivity and quantitation of plasmodium falciparum var gene transcripts by real-time rt-pcr in comparison with conventional rt-pcr.antigenic variation in plasmodium falciparum erythrocyte membrane protein 1, caused by a switch in transcription of the encoding var gene, is an important feature of malaria. in this study, we quantified the relative abundance of var gene transcripts present in p. falciparum parasite clones using real-time reverse transcription-polymerase chain reaction (rt-pcr) and conventional rt-pcr combined with cloning and sequencing, with the aim of directly comparing the results obtained. when there was s ...200616896121
an outbreak of salmonella abortusovis abortions in sheep in south croatia.the aim of the study was to give an account of the epidemic of abortions in sheep caused by salmonella enterica ssp. enterica serovar abortusovis, which occurred in dalmatia, south croatia, in winter 2003-2004. five sheep flocks with rate of abortion ranging from 22% to 38% during the last-third of gestation were examined. salmonella abortusovis was isolated from 13 vaginal smears and two fetuses. direct inoculation was found to be superior to pre-enrichment and enrichment in selective broth for ...200616907961
broilers do not play a dominant role in the campylobacter fetus contamination of humans.campylobacter fetus causes severe infections in humans and can be isolated from various mammals and reptiles. however, although poultry are considered to be the main reservoir of campylobacter jejuni, little is known about the presence of c. fetus in poultry. thus, specific pathogen-free chickens were experimentally inoculated with a mixture of either three non-thermotolerant or four thermotolerant human strains of c. fetus. faecal samples were regularly sampled after inoculation and caeca and i ...200616914660
first case of bacteremia and multifocal cellulitis due to helicobacter canis in an immunocompetent patient.bacteremia due to helicobacter canis has been reported in a patient with x-linked hypogammaglobulinemia. here we report on the first human case of h. canis bacteremia in an immunocompetent host. identification of the organism was made by genetic and phylogenetic analyses of the complete 16s rrna sequence.200617005753
cellulitis and septic arthritis caused by campylobacter fetus and campylobacter jejuni: report of 2 cases and review of the literature.bacteremia caused by gram-negative bacteria occasionally causes soft tissue infections, including cellulitis and septic arthritis. we describe 1 patient each with campylobacter fetus cellulitis and septic arthritis and review the world literature with regard to c. fetus and c. jejuni infections at these sites. altogether, 14 patients with cellulitis (12 due to c. fetus and 2 due to c. jejuni) and 20 patients with septic arthritis (15 due to c. fetus and 4 caused by c. jejuni) have been described ...200317043445
specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms.specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ...200717109769
specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms.specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ...200717109769
temperate bacteriophages affect pulsed-field gel electrophoresis patterns of campylobacter jejuni.the recently sequenced genome of campylobacter jejuni rm1221 revealed the presence of three integrated bacteriophage-like elements. in this study, genes from the first element, a mu-like bacteriophage, were amplified by pcr and used to probe pulsed-field gels of clinical c. jejuni strains obtained from a waterborne outbreak (ontario, canada, 2000). these highly similar strains differed only by their pulsed-field gel electrophoresis (pfge) patterns due to an apparent insertion or deletion of a 40 ...200717135440
temperate bacteriophages affect pulsed-field gel electrophoresis patterns of campylobacter jejuni.the recently sequenced genome of campylobacter jejuni rm1221 revealed the presence of three integrated bacteriophage-like elements. in this study, genes from the first element, a mu-like bacteriophage, were amplified by pcr and used to probe pulsed-field gels of clinical c. jejuni strains obtained from a waterborne outbreak (ontario, canada, 2000). these highly similar strains differed only by their pulsed-field gel electrophoresis (pfge) patterns due to an apparent insertion or deletion of a 40 ...200717135440
capa, an autotransporter protein of campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut.two putative autotransporter proteins, capa and capb, were identified in silico from the genome sequence of campylobacter jejuni nctc11168. the genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slipped-strand mispairing mechanism; in each case the gene sequence contained frameshifts at these positions. the c-terminal two-thirds of the two genes, as well as a portion of the predicted signal peptides, were identical; the remaining n-terminal port ...200717172331
capa, an autotransporter protein of campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut.two putative autotransporter proteins, capa and capb, were identified in silico from the genome sequence of campylobacter jejuni nctc11168. the genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slipped-strand mispairing mechanism; in each case the gene sequence contained frameshifts at these positions. the c-terminal two-thirds of the two genes, as well as a portion of the predicted signal peptides, were identical; the remaining n-terminal port ...200717172331
oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type.the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ...200717172349
oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type.the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ...200717172349
[bacteraemia due to campylobacter fetus in an immune suppressed patient].we report a case of bacteraemia by campylobacter fetus subsp. fetus in a 77 year-old woman with immunosuppression secondary to steroid use. diagnosis was suspected by finding gram negative curved rods in blood cultures taken after 4 days of a febrile illness without local findings. diarrhea was not present. there was no consumption of undercooked meat or non-pasteurized milk and no contact with pets. the patient was treated with sulbactam-cefoperazone due to the coexistence of urinary tract infe ...200617186081
s-layer anchoring and localization of an s-layer-associated protease in caulobacter crescentus.the s-layer of the gram-negative bacterium caulobacter crescentus is composed of a single protein, rsaa, that is secreted and assembled into a hexagonal crystalline array that covers the organism. despite the widespread occurrence of comparable bacterial s-layers, little is known about s-layer attachment to cell surfaces, especially for gram-negative organisms. having preliminary indications that the n terminus of rsaa anchors the monomer to the cell surface, we developed an assay to distinguish ...200717209028
comparison of disk diffusion and agar dilution methods for erythromycin and ciprofloxacin susceptibility testing of campylobacter jejuni subsp. jejuni.disk diffusion was a reliable, easy, and inexpensive method for testing the susceptibility of campylobacter jejuni to erythromycin (215 susceptible and 45 resistant isolates) and to ciprofloxacin (154 susceptible, two intermediate, and 124 resistant isolates) using, respectively, an erythromycin disk and ciprofloxacin and nalidixic acid disks.200717261628
[bacterial meningitis with campylobacter fetus manifesting chronic clinical course].a 43-year-old man with no past history of underlying disease was admitted to an affiliated hospital, complaining of high fever and severe headache. polynuclear dominant pleocytosis in the cerebrospinal fluid (csf) suggested bacterial meningitis. he was immediately treated with several antibiotics, then his clinical symptoms improved, although intractable headache relapsed after withdrawal of the initial therapy. thereafter he consulted our hospital for a second opinion. csf examination in our ho ...200617323778
management of an abdominal aortic aneurysm infected with campylobacter fetus: a case report.we present a rare case of an abdominal aortic aneurysm (aaa) infected with campylobacter fetus. the patient presented with abdominal pain and leukocytosis, without a palpable aaa. computed tomography (ct) of the abdomen showed a 3.1 x 3.0 cm infrarenal abdominal aneurysm with an extra-aortic fluid collection. at surgery, an in situ graft was placed. intraoperative aortic wall cultures grew pansensitive c. fetus, and blood cultures remained negative. at 9-month follow-up, the patient was doing we ...200717349352
campylobacter reactive arthritis: a systematic review.to review the literature on the epidemiology of campylobacter-associated reactive arthritis (rea).200717360026
thermophilic campylobacter spp. in salad vegetables in malaysia.the main aim of this study was to combine the techniques of most probable number (mpn) and polymerase chain reaction (pcr) for quantifying the prevalence and numbers of campylobacter spp. in ulam, a popular malaysian salad dish, from a traditional wet market and two modern supermarkets in selangor, malaysia. a total of 309 samples of raw vegetables which are used in ulam were examined in the study. the prevalences of campylobacters in raw vegetables were, for supermarket i, campylobacter spp., 5 ...200717399832
in vitro activities of cg400549, a novel fabi inhibitor, against recently isolated clinical staphylococcal strains in korea.the in vitro activities of cg400549, a novel fabi inhibitor, were compared to those of linezolid and commonly used antimicrobials against recent bacterial isolates. cg400549 had an mic(90) of 0.5 microg/ml for staphylococcus aureus strains and was more potent than either linezolid or vancomycin.200717420210
[campylobacter fetus bacteremia and cellulitis complicating a venous access port infection in an hiv infected patient].campylobacter fetus subspecies fetus is an opportunist gram-negative bacillus, which is known to be a cause of systemic infections, mainly in immunocompromised patients. we report a c. fetus bacteremia and cellulitis complicating a venous access port infection in a patient with acquired immunodeficiency syndrome (aids). this bacillus seems to have a predilection for the vascular endothelium and its isolation is difficult. physicians should be aware of c. fetus infection in patients with vascular ...200717446026
fatal relapse of a purulent pleurisy caused by campylobacter fetus subsp. fetus.campylobacter fetus is associated with invasive disease, while other campylobacter species, such as c. coli and c. jejuni, are a common cause of bacterial diarrhea. bacteremia has been well described, but pleurisy remains very uncommon. we report the recurrent isolation of a c. fetus subsp. fetus strain during two episodes of pleural effusion with a fatal outcome.200717507518
development of experimental genetic tools for campylobacter fetus.molecular analysis of the virulence mechanisms of the emerging pathogen campylobacter fetus has been hampered by the lack of genetic tools. we report the development and functional analysis of escherichia coli-campylobacter shuttle vectors that are appropriate for c. fetus. some vectors were constructed based on the known campylobacter coli plasmid pip1455 replicon, which confers a wide host range in campylobacter spp. versatility in directing gene expression was achieved by introducing a strong ...200717513579
infection of the thyroid gland caused by campylobacter fetus subsp. fetus.we report what we consider to be the first case of an abscess of the thyroid gland due to campylobacter fetus subsp. fetus (c. fetus) in a patient suffering from hyperthyroidism. c. fetus is known as a rare and opportunistic pathogen in humans, causing a broad variety of systemic infections. acquisition by humans is thought to occur through contact with animals or animal products and to start as a gastro-intestinal colonization. the detection of c. fetus in stool is challenging, since culture ef ...200717547296
a cyclic amp receptor protein-regulated cell-cell communication system mediates expression of a feca homologue in stenotrophomonas maltophilia.stenotrophomonas maltophilia wr-c possesses an rpf/diffusible signal factor (dsf) cell-cell communication system. it produces cis-delta2-11-methyl-dodecenoic acid, a dsf, and seven structural derivatives, which require rpff and rpfb for synthesis. acquisition of iron from the environment is important for bacterial growth as well as the expression of virulence genes. we identified a gene homologous to feca, which encodes a ferric citrate receptor that transports exogenous siderophore ferric citra ...200717574998
c-reactive protein collaborates with plasma lectins to boost immune response against bacteria.although human c-reactive protein (crp) becomes upregulated during septicemia, its role remains unclear, since purified crp showed no binding to many common pathogens. contrary to previous findings, we show that purified human crp (hcrp) binds to salmonella enterica, and that binding is enhanced in the presence of plasma factors. in the horseshoe crab, carcinoscorpius rotundicauda, crp is a major hemolymph protein. incubation of hemolymph with a range of bacteria resulted in crp binding to all t ...200717581635
[bacteremia due to campylobacter fetus isolated by conventional methods from an immunocompromised patient].the genus campylobacter includes gram-negative, motile, curved rods that can evidence characteristic morphologies. these microorganisms require low oxygen tension and an increased level of co2 for growing. a case of bacteremia due to campylobacter fetus in a patient with a previous diagnosis of breast cancer with metastases in dorso-lumbar column and acute promyelocytic leukemia (fab-m3 variant) is presented. the patient was admitted to our institution due to loss of consciousness and a 2 day--h ...200717585657
multicenter study to evaluate bloodstream infection by helicobacter cinaedi in japan.helicobacter cinaedi has being recognized as an important human pathogen which causes bloodstream infections. although the first case of bacteremia with this pathogen in japan was reported in 2003, the true prevalence of h. cinaedi as a pathogen of bloodstream infections in this country is not yet known. therefore, the aim of our study was to assess the incidence of bacteremia with h. cinaedi in japan. we conducted a prospective, multicenter analysis in 13 hospitals during 6 months in tokyo, jap ...200717596362
high content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm olavius algarvensis.selenocysteine (sec) and pyrrolysine (pyl) are rare amino acids that are cotranslationally inserted into proteins and known as the 21st and 22nd amino acids in the genetic code. sec and pyl are encoded by uga and uag codons, respectively, which normally serve as stop signals. herein, we report on unusually large selenoproteomes and pyrroproteomes in a symbiont metagenomic dataset of a marine gutless worm, olavius algarvensis. we identified 99 selenoprotein genes that clustered into 30 families, ...200717626042
a case of mitral endocarditis due to campylobacter fetus subsp. fetus.this report describes a patient presenting mitral native endocarditis due to campylobacter fetus subsp. fetus, which was revealed by syncope and identified using 16s ribosomal rna gene sequencing. this gene sequencing method has become the preferred approach to identifying the new emerging pathogens when discrepancies exist between phenotypical tests.200717642532
development of a real-time fluorescence resonance energy transfer pcr to detect arcobacter species.a real-time pcr targeting the gyrase a subunit gene outside the quinolone resistance-determining region has been developed to detect arcobacter species. the species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. discrimination between arcobacter species was straightforward, as the corresponding melting points showed significant differences with the characteristic melting temperatures of 63.5 degrees c, 58.4 degr ...200717652482
discovery of insertion element iscfe1: a new tool for campylobacter fetus subspecies differentiation.the species campylobacter fetus is divided into the subspecies c. fetus subsp. venerealis (cfv) and c. fetus subsp. fetus (cff). cfv is the causative agent of bovine genital campylobacteriosis, a highly contagious venereal disease that may lead to serious reproductive problems, including sterility and abortion. in contrast, cff can be isolated from the gastrointestinal tract of a wide range of host species, is associated with abortion in sheep and cattle, and can also be isolated from local and ...200717697006
genome dynamics of campylobacter jejuni in response to bacteriophage predation.campylobacter jejuni is a leading cause of food-borne illness. although a natural reservoir of the pathogen is domestic poultry, the degree of genomic diversity exhibited by the species limits the application of epidemiological methods to trace specific infection sources. bacteriophage predation is a common burden placed upon c. jejuni populations in the avian gut, and we show that amongst c. jejuni that survive bacteriophage predation in broiler chickens are bacteriophage-resistant types that d ...200717722979
development of a multiplex pcr assay for identification of campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis.a multiplex pcr assay has been developed for the identification of the six common campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis. the assay was developed using a combination of newly designed and published primers. it provided a specific pcr product for each of the five campylobacter species and ...200717965346
house-level risk factors associated with the colonization of broiler flocks with campylobacter spp. in iceland, 2001 - 2004.the concurrent rise in consumption of fresh chicken meat and human campylobacteriosis in the late 1990's in iceland led to a longitudinal study of the poultry industry to identify the means to decrease the frequency of broiler flock colonization with campylobacter. because horizontal transmission from the environment is thought to be the most likely source of campylobacter to broilers, we aimed to identify broiler house characteristics and management practices associated with flock colonization. ...200717997846
campylobacter fetus bloodstream infection: risk factors and clinical features.in this paper, we report 21 cases of campylobacter fetus bloodstream infection observed in our institution over a 9-year period. the median age of the patients was 78 years. most of them (62%) had a significant underlying disease, such as diabetes, immunodeficiency or cardiovascular disease. the main clinical features were fever with (62% of cases) or without (38%) extra-intestinal symptoms. these included mycotic aneurysm of the abdominal aorta (24%) and cellulitis (19%). antibiotic treatment w ...200817999095
wide geographical distribution of internationally rare campylobacter clones within new zealand.during the southern hemisphere winter of 2006 new zealand experienced a significant increase in the number of reported cases of campylobacter infection. in total, 112 campylobacter isolates from eight district health boards (dhbs) located across new zealand were submitted for pfge, mlst and penner serotyping analysis. distinct clusters of campylobacter isolates were identified, several of which were composed of isolates from up to five different dhbs located on both the north and south islands o ...200818028582
wide geographical distribution of internationally rare campylobacter clones within new zealand.during the southern hemisphere winter of 2006 new zealand experienced a significant increase in the number of reported cases of campylobacter infection. in total, 112 campylobacter isolates from eight district health boards (dhbs) located across new zealand were submitted for pfge, mlst and penner serotyping analysis. distinct clusters of campylobacter isolates were identified, several of which were composed of isolates from up to five different dhbs located on both the north and south islands o ...200818028582
the complete genome sequence and analysis of the epsilonproteobacterium arcobacter butzleri.arcobacter butzleri is a member of the epsilon subdivision of the proteobacteria and a close taxonomic relative of established pathogens, such as campylobacter jejuni and helicobacter pylori. here we present the complete genome sequence of the human clinical isolate, a. butzleri strain rm4018.200718159241
retrospective study of campylobacter infection in a zoological collection.little is known about the epidemiology of campylobacter spp. in wild animal populations. however, zoological collections can provide valuable insights. using records from the zoological society of london whipsnade zoo compiled between 1990 and 2003, the roles of a range of biotic and abiotic factors associated with the occurrence of campylobacteriosis were investigated. the occurrence of campylobacteriosis varied widely across host taxonomic orders. furthermore, in mammals, a combination of chan ...200818165368
retrospective study of campylobacter infection in a zoological collection.little is known about the epidemiology of campylobacter spp. in wild animal populations. however, zoological collections can provide valuable insights. using records from the zoological society of london whipsnade zoo compiled between 1990 and 2003, the roles of a range of biotic and abiotic factors associated with the occurrence of campylobacteriosis were investigated. the occurrence of campylobacteriosis varied widely across host taxonomic orders. furthermore, in mammals, a combination of chan ...200818165368
computational biology in argentina. 200718166076
arthritis risk after acute bacterial gastroenteritis.reactive arthritis (rea) may occur from bacterial gastroenteritis. we studied the risk of arthritis after an outbreak of escherichia coli o157:h7 and campylobacter species within a regional drinking water supply to examine the relationship between the severity of acute diarrhoea and subsequent symptoms of arthritis.200818184664
moraxella osloensis gene expression in the slug host deroceras reticulatum.the bacterium moraxella osloensis is a mutualistic symbiont of the slug-parasitic nematode phasmarhabditis hermaphrodita. in nature, p. hermaphrodita vectors m. osloensis into the shell cavity of the slug host deroceras reticulatum in which the bacteria multiply and kill the slug. as m. osloensis is the main killing agent, genes expressed by m. osloensis in the slug are likely to play important roles in virulence. studies on pathogenic interactions between bacteria and lower order hosts are few, ...200818226222
campylobacter fetus infection of an internal cardioverter defibrillator.infection is a serious complication post implantation of an implantable cardiac defibrillator (icd). we report a case of a 77-year-old man who had an icd generator change complicated by a hematoma. the generator became infected at the time of campylobacter fetus bacteremia. the icd was removed and a new device was implanted without complication.200818233985
evidence for the involvement of infectious agents in the pathogenesis of crohn's disease.many advances have been made in the understanding of crohn's disease (cd) pathogenesis during the last decade. cd is currently seen as a predominantly t-lymphocyte-driven disease characterized by the presence of a complex cocktail of interacting cytokines, chemokines and other mediators produced by a variety of cell types. prevailing theories of cd pathogenesis suggest that patients' t-lymphocytes are inappropriately activated in the setting of an immune imbalance, which is itself caused by an u ...200818240341
the macaque gut microbiome in health, lentiviral infection, and chronic enterocolitis.the vertebrate gut harbors a vast community of bacterial mutualists, the composition of which is modulated by the host immune system. many gastrointestinal (gi) diseases are expected to be associated with disruptions of host-bacterial interactions, but relatively few comprehensive studies have been reported. we have used the rhesus macaque model to investigate forces shaping gi bacterial communities. we used dna bar coding and pyrosequencing to characterize 141,000 sequences of 16s rrna genes ob ...200818248093
development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex pcr assay for the detection and identification of campylobacter jejuni, campylobacter coli and campylobacter fetus.a cytolethal distending toxin (cdt) gene-based species-specific multiplex pcr assay for the detection of cdta, cdtb or cdtc gene of campylobacter jejuni, campylobacter coli or campylobacter fetus, respectively, was developed and evaluated with 76 campylobacter strains belonging to seven different species and 131 other bacterial strains of eight different genera. the cdta, cdtb or cdtc gene of c. jejuni, c. coli or c. fetus, respectively, could be successfully amplified using the corresponding se ...200818248435
development and evaluation of immunochromatographic assay for simple and rapid detection of campylobacter jejuni and campylobacter coli in human stool specimens.an immunochromatographic assay (campy-ica) using a newly generated single monoclonal antibody against a 15-kda cell surface protein of campylobacter jejuni was developed. when cell suspensions of 86 c. jejuni strains and 27 campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the campy-ica, they all yielded positive results. the minimum detectable limits for the c. jejuni strains ranged from 1.8 x 1 ...200818256225
development of a strain-specific molecular method for quantitating individual campylobacter strains in mixed populations.the identification of sites resulting in cross-contamination of poultry flocks in the abattoir and determination of the survival and persistence of campylobacters at these sites are essential for the development of intervention strategies aimed at reducing the microbial burden on poultry at retail. a novel molecule-based method, using strain- and genus-specific oligonucleotide probes, was developed to detect and enumerate specific campylobacter strains in mixed populations. strain-specific oligo ...200818281428
emergence of a tetracycline-resistant campylobacter jejuni clone associated with outbreaks of ovine abortion in the united states.campylobacter infection is one of the major causes of ovine abortions worldwide. historically, campylobacter fetus subsp. fetus was the major cause of campylobacter-associated abortion in sheep; however, campylobacter jejuni is increasingly associated with sheep abortions. we examined the species distribution, genotypes, and antimicrobial susceptibilities of abortion-associated campylobacter isolates obtained from multiple lambing seasons on different farms in iowa, idaho, south dakota, and cali ...200818322054
evaluation of 16s rrna gene-based pcr assays for genus-level identification of helicobacter species.the inclusivity, exclusivity, and detection limit of six 16s rrna gene-based helicobacter genus-specific pcr assays were examined. five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). the clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.200818337392
sequence variability of campylobacter temperate bacteriophages.prophages integrated within the chromosomes of campylobacter jejuni isolates have been demonstrated very recently. prior work with campylobacter temperate bacteriophages, as well as evidence from prophages in other enteric bacteria, suggests these prophages might have a role in the biology and virulence of the organism. however, very little is known about the genetic variability of campylobacter prophages which, if present, could lead to differential phenotypes in isolates carrying the phages ve ...200818366706
fibronectin enhances campylobacter fetus interaction with extracellular matrix components and int 407 cells.campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. no adhesins specific for c. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. scanning electron microscopy revealed c. fetus attachment to the serosal surface of human colonic biopsy explants, a location consistent with the presence of the extracellular matrix (ecm). to determine whether the ecm mediated c. fetus adherence, 7 c. fetus st ...200818388970
infected aneurysms of bilateral deep femoral arteries due to campylobacter fetus subspecies fetus.infected aneurysms due to campylobacter fetus subspecies fetus have rarely been reported. here, we describe the first case of infected aneurysm of bilateral deep femoral arteries due to c. fetus fetus. we successfully treated this case by administration of antibiotics effective for c. fetus fetus and bilateral obturator bypass with complete resection of the infected aneurysms. the aneurysmal wall culture disclosed the presence of c. fetus fetus in a microaerobic atmosphere after the operation. a ...200818466824
sensitive and rapid detection of cholera toxin-producing vibrio cholerae using a loop-mediated isothermal amplification.vibrio cholerae is widely acknowledged as one of the most important waterborne pathogen causing gastrointestinal disorders. cholera toxin (ct) is a major virulence determinant of v. cholerae. detection of ct-producing v. cholerae using conventional culture-, biochemical- and immunological-based assays is time-consuming and laborious, requiring more than three days. thus, we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for the sensitive and rapid detec ...200818547441
[case of bilateral subdural empyema complicating campylobacter fetus subspecies fetus meningitis].campylobacter fetus subspecies fetus (c. fetus) is a relatively rare cause of meningitis, with only 3 cases of c. fetus subdural empyema reported to date. we experienced a case of bilateral subdural empyema complicating c. fetus meningitis. a 51-year-old man was found lying unconscious on a street and was brought to the hospital in an ambulance. he had no past history of any underlying disease but he had suffered head trauma approximately 1 month prior to this episode. on admission, he had high ...200818567362
identification of a gene encoding a transporter essential for utilization of c4 dicarboxylates in corynebacterium glutamicum.the corynebacterium glutamicum r genome contains a total of eight genes encoding proteins with sequence similarity to c4-dicarboxylate transporters identified from other bacteria. three of the genes encode proteins within the dicarboxylate/amino acid:cation symporter (daacs) family, another three encode proteins within the tripartite atp-independent periplasmic transporter family, and two encode proteins within the divalent anion:na+ symporter (dass) family. we observed that a mutant strain defi ...200818586971
design and construction of a double inversion recombination switch for heritable sequential genetic memory.inversion recombination elements present unique opportunities for computing and information encoding in biological systems. they provide distinct binary states that are encoded into the dna sequence itself, allowing us to overcome limitations posed by other biological memory or logic gate systems. further, it is in theory possible to create complex sequential logics by careful positioning of recombinase recognition sites in the sequence.200818665232
synonymous and nonsynonymous polymorphisms versus divergences in bacterial genomes.comparison of the ratio of nonsynonymous to synonymous polymorphisms within species with the ratio of nonsynonymous to synonymous substitutions between species has been widely used as a supposed indicator of positive darwinian selection, with the ratio of these 2 ratios being designated as a neutrality index (ni). comparison of genome-wide polymorphism within 12 species of bacteria with divergence from an outgroup species showed substantial differences in ni among taxa. a low level of nonsynonym ...200818667439
unveiling novel reco distant orthologues involved in homologous recombination.the generation of a reca filament on single-stranded dna is a critical step in homologous recombination. two main pathways leading to the formation of the nucleofilament have been identified in bacteria, based on the protein complexes mediating reca loading: recbcd (addab) and recfor. many bacterial species seem to lack some of the components involved in these complexes. the current annotation of the helicobacter pylori genome suggests that this highly diverse bacterial pathogen has a reduced se ...200818670631
necessary corrections to the approved lists of bacterial names according to rule 40d (formerly rule 46). opinion 86.the judicial commission affirms that, according to rule 40d, formerly rule 46, of the bacteriological code, the authorship of a number of subspecies names included on the approved lists of bacterial names must be corrected. these names are acetobacter aceti subsp. aceti, acetobacter pasteurianus subsp. pasteurianus, bacteroides melaninogenicus subsp. melaninogenicus, campylobacter fetus subsp. fetus, mycobacterium chelonae subsp. chelonae, propionibacterium freudenreichii subsp. freudenreichii, ...200818676492
[infected abdominal aortic aneurysm from campylobacter fetus bacteremia]. 200818681158
aortoiliac aneurysms infected by campylobacter fetus.few reports of aortoiliac aneurysms infected by campylobacter fetus are available. we report five cases and review previous reports, with a view to describing the clinical pattern, treatment options, and outcome of this infection.200818692356
campylobacter bacteremia: clinical features and factors associated with fatal outcome.campylobacter bacteremia is uncommon. the influence of underlying conditions and of the impact of antibiotics on infection outcome are not known.200818699745
campylobacter fetus meningitis in a patient with crohn's disease. 200918798576
development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of vibrio parahaemolyticus.vibrio parahaemolyticus is a marine seafood-borne pathogen causing gastrointestinal disorders in humans. thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are known as major virulence determinants of v. parahaemolyticus. most v. parahaemolyticus isolates from the environment do not produce tdh or trh. total v. parahaemolyticus has been used as an indicator for control of seafood contamination toward prevention of infection. detection of total v. parahaemolyticus using conventio ...200818823567
viability of vibrio fetus in petrolatum. 194818863381
a satisfactory medium for the isolation, cultivation, and maintenance of viability of vibrio fetus (bovine). 194818887830
the apparent involvement of vibrio fetus in an infection of man. 194818917668
influence of campylobacter fetus subsp. fetus on ram sperm cell quality.campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of c. fetus subsp. fetus on the male factor. our objective was to examine the influence of c. fetus subsp. fetus on ram sperm. motility index, percentage of live spermatozoa, mean alphat value (an indication of the chromatin stability of the sperm cell) and percentage o ...200818927420
demographic determinants for campylobacter infection in england and wales: implications for future epidemiological studies.despite a significant public health burden the epidemiology of human campylobacter infection remains blurred. the identification of demographic determinants for campylobacter infection is therefore essential for identifying potential areas for intervention. demographic data from an active, population-based sentinel surveillance system for campylobacter infection (from 2000 until 2003, n=15 907) were compared with appropriate denominator data from the 2001 united kingdom census. incidence was hig ...200819000328
campylobacter infection as a trigger for guillain-barré syndrome in egypt.most studies of campylobacter infection triggering guillain-barré syndrome (gbs) are conducted in western nations were campylobacter infection and immunity is relatively rare. in this study, we explored campylobacter infections, campylobacter serotypes, autoantibodies to gangliosides, and gbs in egypt, a country where campylobacter exposure is common.200819002255
rapid evolution and the importance of recombination to the gastroenteric pathogen campylobacter jejuni.responsible for the majority of bacterial gastroenteritis in the developed world, campylobacter jejuni is a pervasive pathogen of humans and animals, but its evolution is obscure. in this paper, we exploit contemporary genetic diversity and empirical evidence to piece together the evolutionary history of c. jejuni and quantify its evolutionary potential. our combined population genetics-phylogenetics approach reveals a surprising picture. campylobacter jejuni is a rapidly evolving species, subje ...200919008526
rapid evolution and the importance of recombination to the gastroenteric pathogen campylobacter jejuni.responsible for the majority of bacterial gastroenteritis in the developed world, campylobacter jejuni is a pervasive pathogen of humans and animals, but its evolution is obscure. in this paper, we exploit contemporary genetic diversity and empirical evidence to piece together the evolutionary history of c. jejuni and quantify its evolutionary potential. our combined population genetics-phylogenetics approach reveals a surprising picture. campylobacter jejuni is a rapidly evolving species, subje ...200919008526
the biofilm forming potential of bacterial species in the genus campylobacter.the biofilm forming abilities of 16 strains representative of 14 of the 16 species comprising the genus campylobacter were determined on glass, stainless steel, and polystyrene plastic. the formation of biofilms has been suggested as a means by which campylobacter is able to persist within an inhospitable environment. of the eight microaerophilic campylobacter species, including two strains each of campylobacter jejuni and campylobacter fetus, only c. jejuni strain 81-176 reliably produced a vis ...200919028304
increased tlr4 expression in murine placentas after oral infection with periodontal pathogens.maternal periodontitis has emerged as a putative risk factor for preterm births in humans. the periodontitis-associated dental biofilm is thought to serve as an important source of oral bacteria and related virulence factors that hematogenously disseminate and affect the fetoplacental unit; however the underlying biological mechanisms are yet to be fully elucidated. this study hypothesized that an oral infection with the human periodontal pathogens campylobacter rectus and porphyromonas gingival ...200919101032
increased tlr4 expression in murine placentas after oral infection with periodontal pathogens.maternal periodontitis has emerged as a putative risk factor for preterm births in humans. the periodontitis-associated dental biofilm is thought to serve as an important source of oral bacteria and related virulence factors that hematogenously disseminate and affect the fetoplacental unit; however the underlying biological mechanisms are yet to be fully elucidated. this study hypothesized that an oral infection with the human periodontal pathogens campylobacter rectus and porphyromonas gingival ...200919101032
development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of campylobacter fetus.we developed a sensitive and rapid loop-mediated isothermal amplification (lamp) assay for detection of campylobacter fetus. this assay provides simpler and more rapid detection of c. fetus than conventional biochemical and pcr assays. the assay correctly detected 60 c. fetus strains but not 55 non-fetuscampylobacter and 30 non-campylobacter strains. the sensitivity of the lamp assay in pure cultures and in a spiked bovine liver specimen was 10-fold more sensitive than that of the conventional p ...200919128899
campylobacter fetus-associated epidural abscess and bacteremia.campylobacter fetus has been isolated as an infrequent cause of abscesses. we report a case of campylobacter fetus epidural abscess and bacteremia in a debilitated elderly man who had a fatal outcome.200919144805
occurrence and characterization of campylobacter in the brazilian production and processing of broilers.twenty-two commercial broiler flocks and their carcasses, totaling 546 samples (450 collected from a poultry farm and 96 from a slaughterhouse), were surveyed for the presence of campylobacter. the positive results for campylobacter among the analyzed samples were homogeneous, yielding 81.8% for cecal droppings, 80.9% for feces, and 80.4% for cloacal swabs. pre-enrichment and direct plating showed that 77.85% and 81.8% of cloacal swabs, respectively, were positive for campylobacter compared to 9 ...200819166063
production, characterization and determination of the real catalytic properties of the putative 'succinate dehydrogenase' from wolinella succinogenes.both the genomes of the epsilonproteobacteria wolinella succinogenes and campylobacter jejuni contain operons (sdhabe) that encode for so far uncharacterized enzyme complexes annotated as 'non-classical' succinate:quinone reductases (sqrs). however, the role of such an enzyme ostensibly involved in aerobic respiration in an anaerobic organism such as w. succinogenes has hitherto been unknown. we have established the first genetic system for the manipulation and production of a member of the non- ...200919170876
isolation of campylobacter fetus subspecies fetus in a two-year-old quarterhorse with chronic diarrhea of an undetermined etiology.a 2-year-old quarterhorse was evaluated for chronic diarrhea and weight loss of 5 weeks duration after numerous diagnostic tests failed to identify an underlying cause. historically, the horse was housed at pasture where human household waste vehicles were routinely cleaned and the effluent could run onto the field. physical examination revealed poor body condition and frequent high-volume diarrhea. diagnostic testing for salmonella spp., endoparasites, cryptosporidium spp., clostridium spp., an ...200919286512
molecular epidemiology and characterization of campylobacter spp. isolated from wild bird populations in northern england.campylobacter infections have been reported at prevalences ranging from 2 to 50% in a range of wild bird species, although there have been few studies that have investigated the molecular epidemiology of campylobacter spp. consequently, whether wild birds are a source of infection in humans or domestic livestock or are mainly recipients of domestic animal strains and whether separate cycles of infection occur remain unknown. to address these questions, serial cross-sectional surveys of wild bird ...200919286781
[isolation of campylobacter fetus from persons with obstetric-gynecological infections].campylobacter fetus subspecies fetus is a cause of different obstetric-gynecological diseases. it is a first time when rate of infection with campylobacter was studied and connection between the infection and development of chronic gynecologic diseases and pathology of labor was established. bacteria were isolated and identified in 36.0% +/- 0.7 of studied women admitted to inpatient clinics. it was established that campylobacter fetus subspecies fetus can cause abnormalities in placenta functio ...200919338242
comparative studies of campylobacter jejuni genomic diversity reveal the importance of core and dispensable genes in the biology of this enigmatic food-borne pathogen.mlst, dna microarrays, and genome sequencing has allowed for a greater understanding of the metabolic capacity and epidemiology of campylobacter jejuni. while strain-specific genes may provide an isolate a selective advantage in environments and contribute to the organism's pathogenicity, recent work indicates that c. jejuni pathogenicity is dictated by variations in the nucleotide sequence of core genes. challenges facing c. jejuni researchers include determining (a) the degree to which genomic ...200919346123
characterization of two homologous disulfide bond systems involved in virulence factor biogenesis in uropathogenic escherichia coli cft073.disulfide bond (dsb) formation is catalyzed by disulfide bond proteins and is critical for the proper folding and functioning of secreted and membrane-associated bacterial proteins. uropathogenic escherichia coli (upec) strains possess two paralogous disulfide bond systems: the well-characterized dsbab system and the recently described dsbli system. in the dsbab system, the highly oxidizing dsba protein introduces disulfide bonds into unfolded polypeptides by donating its redox-active disulfide ...200919376849
improving protein extraction and separation methods for investigating the metaproteome of anaerobic benzene communities within sediments.btex compounds such as benzene are frequent soil and groundwater contaminants that are easily biodegraded under oxic conditions by bacteria. in contrast, benzene is rather recalcitrant under anaerobic conditions. the analysis of anoxic degradation is often hampered by difficult sampling conditions, limited amounts of biomass and interference of matrix compounds with proteomic approaches. in order to improve the procedure for protein extraction we established a scheme consisting of the following ...200919381451
[invasive disease due to campylobacter fetus in an immunocompetent infant]. 200919409252
genomic analysis of campylobacter fetus subspecies: identification of candidate virulence determinants and diagnostic assay targets.campylobacter fetus subspecies venerealis is the causative agent of bovine genital campylobacteriosis, asymptomatic in bulls the disease is spread to female cattle causing extensive reproductive loss. the microbiological and molecular differentiation of c. fetus subsp. venerealis from c. fetus subsp. fetus is extremely difficult. this study describes the analysis of the available c. fetus subsp. venerealis azul-94 strain genome (approximately 75-80%) to identify elements exclusively found in c. ...200919422718
rapid identification of bacterial pathogens using a pcr- and microarray-based assay.during the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. we have developed a method based on a modified broad-range pcr and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. the broad-range pcr primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrb and pare. the primer design a ...200919664269
bilateral internal iliac artery aneurysm infected with campylobacter fetus.this is a case report of a patient with campylobacter fetus involving bilateral internal iliac artery aneurysms. the patient was treated successfully by ligation of the bilateral iliac artery aneurysms and antibiotics. according to a review of the english-language medical literature, this was the first such patient to be reported. a 69-year-old african-american male presented with a past medical history of repair of a 6.6 cm abdominal aortic aneurysm. it had been repaired with a dacron bifurcate ...200919698305
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