Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| effect of administration route and dose escalation on plasma and intestinal concentrations of enrofloxacin and ciprofloxacin in broiler chickens. | the (mis)use of fluoroquinolones in the fowl industry has led to an alarming incidence of fluoroquinolone resistance in pathogenic as well as commensal bacteria. next to simply reducing antimicrobial consumption, optimizing dosage regimens can be regarded as a suitable strategy to reduce antimicrobial resistance development without jeopardizing therapy efficacy and outcome. a first step in order to limit antimicrobial resistance is to assess the exposure of the intestinal microbiota to enrofloxa ... | 2014 | 25440469 |
| exploring pfge for detecting large plasmids in campylobacter jejuni and campylobacter coli isolated from various retail meats. | campylobacter spp. is one of the most prevalent bacterial pathogens in retail meat, particularly poultry, and is a leading cause of diarrhea in humans. studies related to campylobacter large plasmids are limited in the literature possibly due to difficulty in isolating them using available alkaline lysis methods. the objectives of this study were to determine the prevalence of plasmids, particularly large ones, in campylobacter spp. isolated from various oklahoma retail meats, and to explore pfg ... | 2014 | 25436507 |
| systematic review and meta-analysis of the proportion of campylobacter cases that develop chronic sequelae. | understanding of chronic sequelae development after campylobacter infection is limited. the objective of the study was to determine via systematic review and meta-analysis the proportion of campylobacter cases that develop chronic sequelae. | 2014 | 25416162 |
| first case report of fatal sepsis due to campylobacter upsaliensis. | we encountered a rare case of severe fatal infection in a 70-year-old woman due to campylobacter upsaliensis, identified by pcr amplification and sequencing analysis of the 16s rrna gene using dna extracted from the isolates. to our knowledge, fatal sepsis due to this organism has never been described to date. | 2014 | 25411172 |
| first case report of fatal sepsis due to campylobacter upsaliensis. | we encountered a rare case of severe fatal infection in a 70-year-old woman due to campylobacter upsaliensis, identified by pcr amplification and sequencing analysis of the 16s rrna gene using dna extracted from the isolates. to our knowledge, fatal sepsis due to this organism has never been described to date. | 2014 | 25411172 |
| prevalence and antimicrobial susceptibility of campylobacter spp. in oklahoma conventional and organic retail poultry. | campylobacter is one of the most important foodborne pathogens that cause bacterial gastroenteritis.this study was conducted to investigate the prevalence and antimicrobial resistance of campylobacter in conventional and organic retail poultry samples purchased from grocery stores in tulsa, oklahoma.one hundred and fifty six chilled retail chicken samples (85 conventional and 71 organic) and 65 chilled retail conventional turkey samples were collected in this study. the prevalence of campylobact ... | 2014 | 25408778 |
| intraspecies comparison of streptomyces pratensis genomes reveals high levels of recombination and gene conservation between strains of disparate geographic origin. | streptomyces are widespread bacteria that contribute to the terrestrial carbon cycle and produce the majority of clinically useful antibiotics. while interspecific genomic diversity has been investigated among streptomyces, information is lacking on intraspecific genomic diversity. streptomyces pratensis has high rates of homologous recombination but the impact of such gene exchange on genome evolution and the evolution of natural product gene clusters remains uncharacterized. | 2014 | 25399205 |
| characterization of campylobacter jejuni and campylobacter coli genotypes in poultry flocks by restriction fragment length polymorphism (rflp) analysis. | we describe a simple, rapid, and discriminatory methodology that allows the routine molecular characterization of campylobacter jejuni and campylobacter coli isolates. the proposed approach is built on one of the earliest and simplest molecular typing methods ever, consisting on the analysis of the fragments of different lengths generated by digestion of homologous dna sequences with specific restriction endonucleases, a process known as restriction fragment length polymorphism (rflp) analysis. ... | 2015 | 25399105 |
| multilocus sequence typing of campylobacter jejuni and campylobacter coli from humans and chickens in north-eastern italy. | this paper reports the multilocus sequence typing (mlst) of 57 c. jejuni and c. coli isolates from humans and chickens in italy and the identification of 17 new sequence types (sts). a high genetic diversity was detected among c. jejuni/c. coli and human/chicken isolates, with a predominance of clonal complexes cc21 and cc828. although human sts were not the same as those found in chickens, 3 ccs overlapped between human and chicken isolates. genotyping of campylobacter strains by mlst should be ... | 2014 | 25387294 |
| comparative genomics of the campylobacter lari group. | the campylobacter lari group is a phylogenetic clade within the epsilon subdivision of the proteobacteria and is part of the thermotolerant campylobacter spp., a division within the genus that includes the human pathogen campylobacter jejuni. the c. lari group is currently composed of five species (c. lari, campylobacter insulaenigrae, campylobacter volucris, campylobacter subantarcticus, and campylobacter peloridis), as well as a group of strains termed the urease-positive thermophilic campylob ... | 2014 | 25381664 |
| prevalence of salmonella and campylobacter on broiler chickens from farm to slaughter and efficiency of methods to remove visible fecal contamination. | a study was conducted to investigate the prevalence of salmonella and campylobacter from farm to slaughter. the efficiency of trimming and water spray (490 to 588 kpa pressure) on the removal of visible fecal contamination from broiler carcasses before chilling was also investigated. drag swabs were used to sample litter from the farm houses. samples of ceca and carcasses without and with visible fecal contamination before and after trimming or spray washing of fecal contamination were taken dur ... | 2014 | 25364917 |
| a severe case of persistent diarrhoea associated with arcobacter cryaerophilus but attributed to campylobacter sp. and a review of the clinical incidence of arcobacter spp. | although rarely, arcobacter spp. have been associated with diarrhoea and bacteraemia. we report a persistent case in a healthy 26-year-old spanish male of bloody diarrhoea, which was attributed to campylobacter but in fact was caused by arcobacter cryaerophilus, as determined by sequencing of the rpob gene. the isolate was re-identified by matrix-assisted laser desorption ionization time of flight (maldi-tof) and genotyped for five putative virulence genes and for seven genes included in the arc ... | 2014 | 25356338 |
| a receptor-binding protein of campylobacter jejuni bacteriophage nctc 12673 recognizes flagellin glycosylated with acetamidino-modified pseudaminic acid. | bacteriophage receptor-binding proteins (rbps) confer host specificity. we previously identified a putative rbp (gp047) from the campylobacter lytic phage nctc 12673 and demonstrated that gp047 has a broader host range than its parent phage. while nctc 12673 recognizes the capsular polysaccharide (cps) of a limited number of campylobacter jejuni isolates, gp047 binds to a majority of c. jejuni and related campylobacter coli strains. in this study, we demonstrate that gp047 also binds to acapsula ... | 2015 | 25354466 |
| differences in carbon source utilisation distinguish campylobacter jejuni from campylobacter coli. | campylobacter jejuni and c. coli are human intestinal pathogens that are the most frequent causes of bacterial foodborne gastroenteritis in humans in the uk. in this study, we aimed to characterise the metabolic diversity of both c. jejuni and c. coli using a diverse panel of clinical strains isolated from the uk, pakistan and thailand, thereby representing both the developed and developing world. our aim was to apply multi genome analysis and biolog phenotyping to determine differences in carbo ... | 2014 | 25348335 |
| emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management. | atypical and multidrug resistance, especially esbl and carbapenemase expressing enterobacteriaceae, is globally spreading. therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy. consequently, rapid antibiotic resistance testing is essential. various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data. pcr- and sequencing-based approaches are th ... | 2014 | 25343142 |
| paidb v2.0: exploration and analysis of pathogenicity and resistance islands. | pathogenicity is a complex multifactorial process confounded by the concerted activity of genetic regions associated with virulence and/or resistance determinants. pathogenicity islands (pais) and resistance islands (reis) are key to the evolution of pathogens and appear to play complimentary roles in the process of bacterial infection. while pais promote disease development, reis give a fitness advantage to the host against multiple antimicrobial agents. the pathogenicity island database (paidb ... | 2014 | 25336619 |
| paidb v2.0: exploration and analysis of pathogenicity and resistance islands. | pathogenicity is a complex multifactorial process confounded by the concerted activity of genetic regions associated with virulence and/or resistance determinants. pathogenicity islands (pais) and resistance islands (reis) are key to the evolution of pathogens and appear to play complimentary roles in the process of bacterial infection. while pais promote disease development, reis give a fitness advantage to the host against multiple antimicrobial agents. the pathogenicity island database (paidb ... | 2014 | 25336619 |
| detection of zoonotic pathogens and characterization of novel viruses carried by commensal rattus norvegicus in new york city. | norway rats (rattus norvegicus) are globally distributed and concentrate in urban environments, where they live and feed in closer proximity to human populations than most other mammals. despite the potential role of rats as reservoirs of zoonotic diseases, the microbial diversity present in urban rat populations remains unexplored. in this study, we used targeted molecular assays to detect known bacterial, viral, and protozoan human pathogens and unbiased high-throughput sequencing to identify ... | 2014 | 25316698 |
| metabolomic profiling and antioxidant activity of some acacia species. | metabolomic profiling of different parts (leaves, flowers and pods) of acacia species (acacia nilotica, acacia seyal and acacia laeta) was evaluated. the multivariate data analyses such as principal component analysis (pca) and partial least square-discriminant analysis (pls-da) were used to differentiate the distribution of plant metabolites among different species or different organs of the same species. a. nilotica was characterized with a high content of saponins and a. seyal was characteriz ... | 2014 | 25313274 |
| interaction between campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host. | campylobacter jejuni and campylobacter coli are recognized as the leading causes of human diarrheal disease throughout the development world. unlike human beings, gastrointestinal tract of pigs are frequently colonized by campylobacter to a high level in a commensal manner. the aim of this study was to identify the differences underlying the divergent outcome following campylobacter challenge in porcine versus human host. in order to address this, a comparative in vitro infection model was combi ... | 2014 | 25307769 |
| method-dependent variability in determination of prevalence of campylobacter jejuni and campylobacter coli in canadian retail poultry. | campylobacter is the most frequent cause of bacterial gastroenteritis in canada, and the illness is commonly associated with poultry consumption. whereas canadian retail poultry is often contaminated with campylobacters, studies on the prevalence of this organism are inconsistent due to variability in sampling and microbiological methodology. to determine the current microbiological status of canadian poultry, and to evaluate two commonly used microbiological methods, 348 raw poultry samples wer ... | 2014 | 25285484 |
| emerging technologies for the clinical microbiology laboratory. | in this review we examine the literature related to emerging technologies that will help to reshape the clinical microbiology laboratory. these topics include nucleic acid amplification tests such as isothermal and point-of-care molecular diagnostics, multiplexed panels for syndromic diagnosis, digital pcr, next-generation sequencing, and automation of molecular tests. we also review matrix-assisted laser desorption ionization-time of flight (maldi-tof) and electrospray ionization (esi) mass spe ... | 2014 | 25278575 |
| incidence of and factors associated with false positives in laboratory diagnosis of norovirus infection by amplification of the rna-dependent rna polymerase gene. | conventional reverse transcription-polymerase chain reaction (rt-pcr) amplification of the rna-dependent rna polymerase (rdrp) gene remains a used method for the rapid detection of norovirus (nv) in clinical laboratories. the incidence of and factors associated with false positives in this assay have not been previously evaluated. | 2014 | 25264621 |
| antimicrobial susceptibility profiles and molecular typing of campylobacter jejuni and campylobacter coli isolates from ducks in south korea. | campylobacter is a food-borne zoonotic pathogen that causes human gastroenteritis worldwide. campylobacter bacteria are commensal in the intestines of many food production animals, including ducks and chickens. the objective of the study was to determine the prevalence of campylobacter species in domestic ducks, and the agar dilution method was used to determine resistance of the isolates to eight antibiotics. in addition, multilocus sequence typing (mlst) was performed to determine the sequence ... | 2014 | 25261524 |
| sensitive and rapid detection of campylobacter species from stools of children with diarrhea in japan by the loop-mediated isothermal amplification method. | we detected campylobacter spp. in 5% (20/380) of diarrheal stool samples collected at an outpatient clinic in kyoto using a commercial loop-mediated isothermal amplification (lamp) kit with a fluorescent detection reagent after dna extraction. the sensitivity and specificity were 100% in comparison with those of semi-nested pcr for the differentiation of campylobacter jejuni and campylobacter coli. fourteen of the 20 samples were already determined as c. jejuni by the culture method. all 20 samp ... | 2014 | 25241688 |
| multilocus sequence typing (mlst) and whole-genome mlst of campylobacter jejuni isolates from human infections in three districts during a seasonal peak in finland. | a total of 95 human campylobacter jejuni isolates acquired from domestic infections and collected from three districts in finland during the seasonal peak (june to september) in 2012 were analyzed by pcr-based multilocus sequence typing (mlst) and by whole-genome sequencing (wgs). four predominant sequence types (sts) were detected among the isolates: st-45 (21%) and st-230 (14%, st-45 clonal complex [cc]), st-267 (21%, st-283 cc), and st-677 (19%, st-677 cc). in districts 1 and 3, most of the i ... | 2014 | 25232158 |
| susceptibility to campylobacter infection is associated with the species composition of the human fecal microbiota. | the gut microbiota is essential for human health, but very little is known about how the composition of this ecosystem can influence and respond to bacterial infections. here we address this by prospectively studying the gut microbiota composition before, during, and after natural campylobacter infection in exposed poultry abattoir workers. the gut microbiota composition was analyzed with 16s amplicon sequencing of fecal samples from poultry abattoir workers during the peak season of campylobact ... | 2014 | 25227462 |
| accurate detection of campylobacter spp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory. | campylobacter spp. are fastidious microorganisms, and their detection by culture depends on the freshness of the stool sample and the skills of the laboratory staff. to improve laboratory diagnosis, assays for the detection of specific antigens have been developed. here, we evaluated two assays for the detection of campylobacter spp.-specific antigens, i.e., one immunochromatographic test and one enzyme-linked immunosorbent assay (eia), in 38 frozen campylobacter spp.-positive specimens and pros ... | 2014 | 25215191 |
| genomic evidence for the emergence and evolution of pathogenicity and niche preferences in the genus campylobacter. | the genus campylobacter includes some of the most relevant pathogens for human and animal health; the continuous effort in their characterization has also revealed new species putatively involved in different kind of infections. nowadays, the available genomic data for the genus comprise a wide variety of species with different pathogenic potential and niche preferences. in this work, we contribute to enlarge this available information presenting the first genome for the species campylobacter sp ... | 2014 | 25193310 |
| evolution and comparative genomics of campylobacter jejuni st-677 clonal complex. | campylobacter is the most common bacterial cause of gastroenteritis in the european union with over 200,000 laboratory-confirmed cases reported annually. this is the first study to describe findings related to comparative genomics analyses of the sequence type (st)-677 clonal complex (cc), a campylobacter jejuni lineage associated with bacteremia cases in humans. we performed whole-genome sequencing, using illumina hiseq sequencing technology, on five related st-677 cc isolates from two chicken ... | 2014 | 25193305 |
| phenotypic and genotypic diversity of thermophilic campylobacter spp. in commercial turkey flocks: a longitudinal study. | poultry are recognized as a main reservoir of campylobacter spp. however, longitudinal studies investigating the persistence of campylobacter on commercial meat turkeys are rare. the objectives of this study were to determine the prevalence, antimicrobial susceptibility, and persistence of genotypically related strains of campylobacter spp. recovered from three commercial turkey farms in ohio belonging to a single producer. eight hundred ten samples were collected from birds aged 1 week to slaug ... | 2014 | 25184688 |
| the sweet tooth of bacteria: common themes in bacterial glycoconjugates. | humans have been increasingly recognized as being superorganisms, living in close contact with a microbiota on all their mucosal surfaces. however, most studies on the human microbiota have focused on gaining comprehensive insights into the composition of the microbiota under different health conditions (e.g., enterotypes), while there is also a need for detailed knowledge of the different molecules that mediate interactions with the host. glycoconjugates are an interesting class of molecules fo ... | 2014 | 25184559 |
| isolation of campylobacter from brazilian broiler flocks using different culturing procedures. | conventional culturing methods enable the detection of campylobacter in broiler flocks. however, laboratory culture of campylobacter is laborious because of its fastidious behavior and the presence of competing nontarget bacteria. this study evaluated different protocols to isolate campylobacter from broiler litter, feces, and cloacal and drag swabs. samples taken from commercial brazilian broiler flocks were directly streaked onto preston agar (pa), campy-line agar (cla), and modified charcoal ... | 2014 | 25172927 |
| campylobacter colonization and proliferation in the broiler chicken upon natural field challenge is not affected by the bird growth rate or breed. | the zoonotic association between campylobacter bacteria in poultry and humans has been characterized by decades of research which has attempted to elucidate the epidemiology of this complex relationship and to reduce carriage within poultry. while much work has focused on the mechanisms facilitating its success in contaminating chicken flocks (and other animal hosts), it remains difficult to consistently exclude campylobacter under field conditions. within the united kingdom poultry industry, va ... | 2014 | 25172857 |
| prevalence of thermotolerant campylobacter spp. in farmed hares (lepus europaeus). | thermotolerant campylobacter spp. were isolated from 118/240 (49.2%) rectal swabs from commercially farmed hares (lepus europaeus) in southern italy. using multiplex pcr, campylobacter coli was identified in 118/118 (100%) positive samples, while 17/118 (14.4%) positive samples were also positive for campylobacter jejuni. adult hares had a higher prevalence of infection with campylobacter spp. than juvenile hares. | 2014 | 25168717 |
| identification and characterisation of new campylobacter group iii phages of animal origin. | campylobacter-specific bacteriophages (phages) are considered as an alternative intervention strategy to decrease the level of poultry contamination with campylobacter, a leading cause of gastroenteritis worldwide. eradication efficiency depends primarily on phage-host interaction mediated by phage tail-spike proteins and bacterial receptors. here, this interaction was characterised using tail-spike gene sequence analysis, phage neutralisation by antiserum and host range analysis of newly isolat ... | 2014 | 25168177 |
| investigating the host specificity of campylobacter jejuni and campylobacter coli by sequencing gyrase subunit a. | surveillance and field investigations of campylobacter infections require molecular tools with genetic markers appropriate for tracing purposes, i.e. based on the principle that some campylobacter lineages acquire a host signature under adaptive selection pressure. we developed a sequence-based method targeting the quinolone resistance determining region within the subunit a of dna gyrase (gyra). host specificity was evaluated by characterizing two collections of campylobacter jejuni (n = 430) a ... | 2014 | 25163418 |
| experimental study of the impact of antimicrobial treatments on campylobacter, enterococcus and pcr-capillary electrophoresis single-strand conformation polymorphism profiles of the gut microbiota of chickens. | an experiment was conducted to compare the impact of antimicrobial treatments on the susceptibility of campylobacter, enterococcus faecium and enterococcus faecalis, and on the diversity of broiler microbiota. specific-pathogen-free chickens were first orally inoculated with strains of campylobacter and enterococcus faecium. birds were then orally treated with recommended doses of oxytetracycline, sulfadimethoxine/trimethoprim, amoxicillin or enrofloxacin. faecal samples were collected before, d ... | 2014 | 25142966 |
| a quantitative prioritisation of human and domestic animal pathogens in europe. | disease or pathogen risk prioritisations aid understanding of infectious agent impact within surveillance or mitigation and biosecurity work, but take significant development. previous work has shown the h-(hirsch-)index as an alternative proxy. we present a weighted risk analysis describing infectious pathogen impact for human health (human pathogens) and well-being (domestic animal pathogens) using an objective, evidence-based, repeatable approach; the h-index. this study established the highe ... | 2014 | 25136810 |
| a pre-enrichment step is essential for detection of campylobacter sp. in turbid pond water. | this work aimed to detect campylobacter species from naturally contaminated turbid pond water by pcr. a total of 16 water samples were collected from a turbid village pond. four methods of dna extraction were applied to centrifuge pellets from eight 100 ml pond water samples prior to attempted detection of campylobacter by pcr without an enrichment step. these methods were (1) tris-hcl and sodium dodecyl sulfate followed by phenol:chloroform:isoamylalcohol extraction followed by treatment with d ... | 2014 | 25134901 |
| human, food and animal campylobacter spp. isolated in portugal: high genetic diversity and antibiotic resistance rates. | infections by campylobacter jejuni and campylobacter coli are considered the major cause of bacterial gastroenteritis in humans, with food being the main source of infection. in this study, a total of 196 campylobacter strains (125 isolates from humans, 39 from retail food and 32 from food animal sources) isolated in portugal between 2009 and 2012 were characterised by multilocus sequence typing (mlst) and flaa short variable region (svr) typing. susceptibility to six antibiotics as well as the ... | 2014 | 25130097 |
| genome analysis of bacillus amyloliquefaciens subsp. plantarum ucmb5113: a rhizobacterium that improves plant growth and stress management. | the bacillus amyloliquefaciens subsp. plantarum strain ucmb5113 is a gram-positive rhizobacterium that can colonize plant roots and stimulate plant growth and defense based on unknown mechanisms. this reinforcement of plants may provide protection to various forms of biotic and abiotic stress. to determine the genetic traits involved in the mechanism of plant-bacteria association, the genome sequence of ucmb5113 was obtained by assembling paired-end illumina reads. the assembled chromosome of 3, ... | 2014 | 25119988 |
| the molecular evolution of the qo motif. | quinol oxidation in the catalytic quinol oxidation site (q(o) site) of cytochrome (cyt) bc(1) complexes is the key step of the q cycle mechanism, which laid the ground for mitchell's chemiosmotic theory of energy conversion. bifurcated electron transfer upon quinol oxidation enables proton uptake and release on opposite membrane sides, thus generating a proton gradient that fuels atp synthesis in cellular respiration and photosynthesis. the q(o) site architecture formed by cyt b and rieske iron- ... | 2014 | 25115012 |
| quantitative effects of in-line operations on campylobacter and escherichia coli through two australian broiler processing plants. | campylobacter is an important food borne pathogen, mainly associated with poultry. a lack of through-chain quantitative campylobacter data has been highlighted within quantitative risk assessments. the aim of this study was to quantitatively and qualitatively measure campylobacter and escherichia coli concentration on chicken carcasses through poultry slaughter. chickens (n=240) were sampled from each of four flocks along the processing chain, before scald, after scald, before chill, after chill ... | 2014 | 25104301 |
| detection, identification and quantification of campylobacter jejuni, coli and lari in food matrices all at once using multiplex qpcr. | thermotolerant campylobacter jejuni, coli and lari are recognized as leading food-borne pathogens causing an acute bacterial enteritis worldwide. due to narrow spectrum of their biochemical activity, it is very complicated to distinguish between individual species. for reliable risk assessment, proper incidence evaluation or swift sample analysis regarding individual species, a demand for simple and rapid method for their distinguishing is reasonable. in this study, we evaluated a reliable and s ... | 2014 | 25057300 |
| campylobacter spp. and birds of prey. | a total of 170 birds of prey admitted to two wildlife rescue and rehabilitation centers of italy were examined. birds were divided by diurnal (n = 15) and nocturnal (n = 7) species, sampled by cloacal swabs, and examined for campylobacter spp. by cultural and molecular methods. campylobacter spp. were isolated in 43 out of the 170 (25.3%) birds of prey examined. among these, 43/43 (100%) were identified as campylobacter jejuni and 10/43 (23.3%) were identified as campylobacter coli recovered fro ... | 2014 | 25055637 |
| identifying etiological agents causing diarrhea in low income ecuadorian communities. | continued success in decreasing diarrheal disease burden requires targeted interventions. to develop such interventions, it is crucial to understand which pathogens cause diarrhea. using a case-control design we tested stool samples, collected in both rural and urban ecuador, for 15 pathogenic microorganisms. pathogens were present in 51% of case and 27% of control samples from the urban community, and 62% of case and 18% of control samples collected from the rural community. rotavirus and shige ... | 2014 | 25048373 |
| inactivation of selected bacterial pathogens in dairy cattle manure by mesophilic anaerobic digestion (balloon type digester). | anaerobic digestion of animal manure in biogas digesters has shown promise as a technology in reducing the microbial load to safe and recommended levels. we sought to treat dairy manure obtained from the fort hare dairy farm by investigating the survival rates of bacterial pathogens, through a total viable plate count method, before, during and after mesophilic anaerobic digestion. different microbiological media were inoculated with different serial dilutions of manure samples that were withdra ... | 2014 | 25026086 |
| repetitive sequence variations in the promoter region of the adhesin-encoding gene saba of helicobacter pylori affect transcription. | the pathogenesis of diseases elicited by the gastric pathogen helicobacter pylori is partially determined by the effectiveness of adaptation to the variably acidic environment of the host stomach. adaptation includes appropriate adherence to the gastric epithelium via outer membrane protein adhesins such as saba. the expression of saba is subject to regulation via phase variation in the promoter and coding regions as well as repression by the two-component system arsrs. in this study, we investi ... | 2014 | 25022855 |
| development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. | childhood diarrhoea can be caused by many pathogens that are difficult to assay in the laboratory. molecular diagnostic techniques provide a uniform method to detect and quantify candidate enteropathogens. we aimed to develop and assess molecular tests for identification of enteropathogens and their association with disease. | 2014 | 25022434 |
| prevalence of and risk factors for campylobacter colonisation in broiler flocks at the end of the rearing period in france. | 1. a study was conducted to estimate the prevalence and quantification by species of campylobacter infection in broiler flocks at the end of the rearing period and to identify associated risk factors. 2. a questionnaire about the rearing period was completed and caecal samples were collected from 121 broiler flocks in brittany, france, during 2008. 3. campylobacter was isolated in 87 out of 121 flocks--a prevalence of 71.9% (95% ci, 63.7-80.1%), including 40.5% of campylobacter jejuni and 29.8% ... | 2014 | 25010255 |
| antibiotic susceptibility pattern and genotyping of campylobacter species isolated from children suffering from gastroenteritis. | to study the prevalence and the antimicrobial resistance of campylobacter species isolated from children suffering from gastroenteritis . | 2014 | 25008814 |
| campylobacter infection in a cohort of rural children in moramanga, madagascar. | campylobacter infection is the most common cause of bacterial gastroenteritis in developing countries, including madagascar. reports of pathogenicity have not been consistent and repeated exposures over time seem to lead to the development of protective immunity in developing areas. we conducted this study to support evidence for these hypotheses by exploring the association between infection and age, the reoccurrence of infection and the pathogenicity of campylobacter. | 2014 | 24996559 |
| same-day subtyping of campylobacter jejuni and c. coli isolates by use of multiplex ligation-dependent probe amplification-binary typing. | campylobacteriosis is the most commonly reported form of human bacterial gastroenteritis in the world. sound identification of infectious sources requires subtyping, but the most widely used methods have turnaround times measured in days and require specialist equipment and skills. a multiplex ligation-dependent probe amplification-binary typing (mbit) assay was developed for subtyping campylobacter jejuni and campylobacter coli. it was tested on 245 isolates, including recent isolates from belg ... | 2014 | 24989612 |
| emergence of multidrug-resistant campylobacter species isolates with a horizontally acquired rrna methylase. | antibiotic-resistant campylobacter constitutes a serious threat to public health, and resistance to macrolides is of particular concern, as this class of antibiotics is the drug of choice for clinical therapy of campylobacteriosis. very recently, a horizontally transferrable macrolide resistance mediated by the rrna methylase gene erm(b) was reported in a campylobacter coli isolate, but little is known about the dissemination of erm(b) among campylobacter isolates and the association of erm(b)-c ... | 2014 | 24982085 |
| benefits and risks of antimicrobial use in food-producing animals. | benefits and risks of antimicrobial drugs, used in food-producing animals, continue to be complex and controversial issues. this review comprehensively presents the benefits of antimicrobials drugs regarding control of animal diseases, protection of public health, enhancement of animal production, improvement of environment, and effects of the drugs on biogas production and public health associated with antimicrobial resistance. the positive and negative impacts, due to ban issue of antimicrobia ... | 2014 | 24971079 |
| videotaped behavior as a predictor of clinical outcome in rhesus macaques (macaca mulatta). | understanding the behavior of laboratory nhp facilitates health assessment and clinical care. we sought to characterize the behavior of critically ill rhesus macaques (macaca mulatta) and determine whether specific behaviors or behavioral changes might facilitate the determination of prognosis and clinical endpoints. twenty-two critically-ill subjects were videorecorded after they were removed from the outdoor breeding colony for diagnostic work-up and treatment. subjects were categorized as sur ... | 2014 | 24956211 |
| a national investigation of the prevalence and diversity of thermophilic campylobacter species in agricultural watersheds in canada. | the occurrence and diversity of thermophilic campylobacter species (c. jejuni, coli, and lari) were studied in water samples from four river basins located across canada. these basins located in quebec (bras d'henri), alberta (oldman), ontario (south nation), and british columbia (sumas) represented some of the most intensive farming areas in canada for hog, beef cattle, dairy cattle, and poultry, respectively. this study analyzed 769 water samples collected from 23 monitoring sites with agricul ... | 2014 | 24930011 |
| development of 23 individual taqman® real-time pcr assays for identifying common foodborne pathogens using a single set of amplification conditions. | most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. the aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. the list of targets include, among the others, listeria spp., salmonella spp., shigella spp., escherichia coli spp., campylobacter spp., clostridium spp. and staphylococcus aureus. the accuracy of detection was deter ... | 2014 | 24929880 |
| rapid detection and differentiation of important campylobacter spp. in poultry samples by dot blot and pcr. | the detection of campylobacter, the most commonly reported cause of foodborne gastroenteritis in the european union, is very important for human health. the most commonly recognised risk factor for infection is the handling and/or consumption of undercooked poultry meat. the methods typically applied to evaluate the presence/absence of campylobacter in food samples are direct plating and/or enrichment culture based on the horizontal method for detection and enumeration of campylobacter spp. (iso ... | 2014 | 24929879 |
| the importance of the viable but non-culturable state in human bacterial pathogens. | many bacterial species have been found to exist in a viable but non-culturable (vbnc) state since its discovery in 1982. vbnc cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques. this leads to an underestimation of total viable cells in environmental or clinical samples, and thus poses a risk to public health. in this review, we present recent findings on the vbnc state of human bacterial pathogens. the characte ... | 2014 | 24917854 |
| in depth analysis of the helicobacter pylori cag pathogenicity island transcriptional responses. | the severity of symptoms elicited by the widespread human pathogen helicobacter pylori is strongly influenced by the genetic diversity of the infecting strain. among the most important pathogen factors that carry an increased risk for gastric cancer are specific genotypes of the cag pathogenicity island (cag-pai), encoding a type iv secretion system (t4ss) responsible for the translocation of the caga effector oncoprotein. to date, little is known about the regulatory events important for the ex ... | 2014 | 24892739 |
| genes required for assembly of pili associated with the helicobacter pylori cag type iv secretion system. | helicobacter pylori causes numerous alterations in gastric epithelial cells through processes that are dependent on activity of the cag type iv secretion system (t4ss). filamentous structures termed "pili" have been visualized at the interface between h. pylori and gastric epithelial cells, and previous studies suggested that pilus formation is dependent on the presence of the cag pathogenicity island (pai). thus far, there has been relatively little effort to identify specific genes that are re ... | 2014 | 24891108 |
| antimicrobial resistance among campylobacter spp. strains isolated from different poultry production systems at slaughterhouse level. | the aim of the current work was to evaluate the prevalence and antimicrobial susceptibility of campylobacter spp. isolated from different chicken production systems at the slaughterhouse level. chicken sampling at slaughterhouse was performed for cecum, carcass, and breast meat from flocks of organic (n = 6), extensive indoor (n = 14), and intensive production (n = 14), totaling 34 ceca pools, 64 neck skin pools, and 132 breasts, representing 96,386 chickens. a collection of 167 strains were ide ... | 2014 | 24879708 |
| molecular subtyping and antimicrobial susceptibilities of campylobacter coli isolates from diarrheal patients and food-producing animals in china. | the aim of this study was to determine the molecular subtyping and antimicrobial susceptibility characteristics of campylobacter coli isolates from different sources in china. one hundred thirteen c. coli isolates were subtyped by pulsed-field gel electrophoresis (pfge), multilocus sequence typing (mlst), and pora and flaa short variable region (svr) nucleotide sequences. cluster analysis was performed based on the pfge and sequence types (st). eighty-four pfge patterns (smai) were observed in 1 ... | 2014 | 24844559 |
| a molecular survey of campylobacter jejuni and campylobacter coli virulence and diversity. | the aim of this study was to determine the prevalence of virulence-associated genes and enterobacterial repetitive intergenic consensus pcr (eric-pcr) analysis of campylobacter spp. isolated from children with diarrhea in iran. | 2014 | 24842142 |
| comparison of maldi-tof ms, gene sequencing and the vitek 2 for identification of seventy-three clinical isolates of enteropathogens. | this study was performed to evaluate the analytical and practical performance of the matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) compared to the sequencing method and the vitek 2 system for identification of enteropathogens in the clinical microbiology laboratory. | 2014 | 24822116 |
| supplementation of bolton broth with triclosan improves detection of campylobacter jejuni and campylobacter coli in chicken carcass rinse. | we compared bolton enrichment broth supplemented with antimicrobial triclosan (t-bolton broth) and normal bolton broth for the isolation of campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli) from chicken carcass rinse. whole chickens were rinsed with buffered peptone water prior to enrichment in normal bolton broth or t-bolton broth, followed by inoculation onto modified charcoal-cefoperazone-deoxycholate agar (mccda). suspect colonies were confirmed by pcr. we observed a signific ... | 2014 | 24813626 |
| human diarrhea infections associated with domestic animal husbandry: a systematic review and meta-analysis. | domestic animal husbandry, a common practice globally, can lead to zoonotic transmission of enteric pathogens. however, this risk has received little attention to date. this systematic review and meta-analysis examines the evidence for an association between domestic exposure to food-producing animals and cases of human diarrhea and specific enteric infections. we performed a systematic review of available literature to examine domestic livestock and poultry as risk factors for diarrhea and appl ... | 2014 | 24812065 |
| specific pathogen-free pig herds also free from campylobacter? | as specific pathogen-free (spf) pig herds are designed and managed to prevent specific pig diseases, it might be feasible to expand the list of micro-organisms also including zoonotic pathogens such as campylobacter coli as this agent has its origin in pigs. in a previous survey, 15 of 16 of spf herds were found free from human pathogenic yersinia enterocolitica. accordingly, three nucleus and seven multiplying herds were surveyed for campylobacter to investigate whether the norwegian spf pig py ... | 2015 | 24798507 |
| loads and antimicrobial resistance of campylobacter spp. on fresh chicken meat in nueva ecija, philippines. | this study was performed to determine the prevalence and to semiquantify campylobacter spp. on chicken meat samples at 4 selected local wet markets in nueva ecija, philippines, and to determine the antimicrobial resistance patterns of the campylobacter isolates. out of 120 chicken meat samples, 57 (47.5%) were campylobacter spp. positive. the majority of isolated campylobacter strains were identified as campylobacter coli (54.4%) and 45.6% as campylobacter jejuni. most of these positive samples ... | 2014 | 24795322 |
| marinade with thyme and orange oils reduces salmonella enteritidis and campylobacter coli on inoculated broiler breast fillets and whole wings. | essential oils have been reported to possess antimicrobial properties and therefore have potential usage as natural antimicrobials in food. in a previous study, thyme orange essential oil combination (toc) used at the 0.5% level as a dip application on chicken cut-up parts had a significant antibacterial effect against salmonella and campylobacter. a study was designed to evaluate the effect of salt-phosphate marinade solution containing 0.5% toc to 1) reduce salmonella enteritidis and campyloba ... | 2014 | 24795320 |
| outbreak of campylobacteriosis associated with a long-distance obstacle adventure race--nevada, october 2012. | on october 12, 2012, the nellis air force base public health flight (nellis public health), near las vegas, nevada, was notified by the mike o'callaghan federal medical center (mofmc) emergency department (ed) of three active-duty military patients who went to the ed during october 10-12 with fever, vomiting, and hemorrhagic diarrhea. initial interviews by clinical staff members indicated that all three patients had participated october 6-7 in a long-distance obstacle adventure race on a cattle ... | 2014 | 24785983 |
| molecular epidemiology and antimicrobial susceptibility of campylobacter coli clinical isolates. | campylobacter spp. is a major cause of acute bacterial diarrhea in humans worldwide, and c. coli is responsible for 10% of the cases. | 2014 | 24768104 |
| antimicrobial susceptibility of campylobacter jejuni and campylobacter coli isolates obtained in montreal, quebec, canada, from 2002 to 2013. | from 2002 to 2013 in montreal, quebec, canada, 38 campylobacter coli isolates were more frequently erythromycin, tetracycline, and ciprofloxacin resistant than 440 campylobacter jejuni subsp. jejuni isolates (18.4% versus 1.8%; p = 0.00005), of which the 148 isolates acquired abroad were more frequently erythromycin, tetracycline, and ciprofloxacin resistant than the 292 isolates acquired locally (5.4% versus 0%; p = 0.0001). | 2014 | 24759726 |
| hygromycin b and apramycin antibiotic resistance cassettes for use in campylobacter jejuni. | campylobacter jejuni genetic manipulation is restricted by the limited number of antibiotic resistance cassettes available for use in this diarrheal pathogen. in this study, two antibiotic resistance cassettes were developed, encoding for hygromycin b and apramycin resistance, for use in mutagenesis or for selection of gene expression and complementation constructs in c. jejuni. first, the marker genes were successfully modified to allow for insertional mutagenesis or deletion of a gene-of-inter ... | 2014 | 24751825 |
| comparison of fecalswab and eswab devices for storage and transportation of diarrheagenic bacteria. | using a collection (n = 12) of atcc and known stock isolates, as well as 328 clinical stool specimens, we evaluated the eswab and the new fecalswab liquid-based microbiology (lbm) devices for storing and transporting diarrheagenic bacteria. the stock isolates were stored in these swab devices up to 48 h at refrigeration (4°c) or room (∼25°c) temperature and up to 3 months at -20°c or -70°c. with the clinical stool specimens, the performances of the eswab and fecalswab were compared to those of r ... | 2014 | 24740083 |
| inhibition controls for qualitative real-time pcr assays: are they necessary for all specimen matrices? | a retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time pcr assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. inhibition rates of ≤ 1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue. | 2014 | 24740078 |
| detection and molecular analysis of campylobacter ureolyticus in domestic animals. | previous studies showed the presence of campylobacter ureolyticus in a large proportion of diarrhoeal samples from patients in ireland. this emerging gastrointestinal pathogen was the second most common campylobacter species detected in patients presenting with gastroenteritis, surpassed only by c. jejuni. however, the source of c. ureolyticus infections in humans remains unknown. the aim of this study was to investigate the presence of c. ureolyticus in a range of domestic animals. over a perio ... | 2014 | 24739468 |
| colonisation of a phage susceptible campylobacter jejuni population in two phage positive broiler flocks. | the pathogens campylobacter jejuni and campylobacter coli are commensals in the poultry intestine and campylobacteriosis is one of the most frequent foodborne diseases in developed and developing countries. phages were identified to be effective in reducing intestinal campylobacter load and this was evaluated, in the first field trials which were recently carried out. the aim of this study was to further investigate campylobacter population dynamics during phage application on a commercial broil ... | 2014 | 24733154 |
| perspectives in the use of tannins as alternative to antimicrobial growth promoter factors in poultry. | antibiotics have been included in the formulation of feed for livestock production for more than 40 years as a strategy to improve feed conversion rates and to reduce costs. the use of antimicrobials as growth-promoting factors (agp) in sub-therapeutic doses for long periods is particularly favorable for the selection of antimicrobial resistant microorganisms. in the last years, global concern about development of antimicrobial resistance and transference of resistance genes from animal to human ... | 2014 | 24723916 |
| draft genome sequence of campylobacter ureolyticus strain cit007, the first whole-genome sequence of a clinical isolate. | herein, we present the draft genome sequence of campylobacter ureolyticus. strain cit007 was isolated from a stool sample from an elderly female presenting with diarrheal illness and end-stage chronic renal disease. | 2014 | 24723712 |
| identification of genes involved in the glycosylation of modified viosamine of flagellins in pseudomonas syringae by mass spectrometry. | previously we revealed that flagellin proteins in pseudomonas syringae pv. tabaci 6605 (pta 6605) were glycosylated with a trisaccharide, modified viosamine (mvio)-rhamnose-rhamnose and that glycosylation was required for virulence. we further identified some glycosylation-related genes, including vioa, viob, viot, fgt1, and fgt2. in this study, we newly identified vior and viom in a so-called viosamine island as biosynthetic genes for glycosylation of mvio in pta 6605 by the mass spectrometry ( ... | 2011 | 24710292 |
| a multiplex pcr/ldr assay for simultaneous detection and identification of the niaid category b bacterial food and water-borne pathogens. | enteric pathogens that cause gastroenteritis remain a major global health concern. the goal of this study was to develop a multiplex pcr/ligation detection reaction (ldr) assay for the detection of all niaid category b bacterial food and water-borne pathogens directly from stool specimens. to validate the pcr/ldr assay, clinical isolates of campylobacter spp., vibrio spp., shigella spp., salmonella spp., listeria monocytogenes, yersinia enterocolitica, and diarrheagenic escherichia coli were tes ... | 2014 | 24709368 |
| prevalence, antibiogram and risk factors of thermophilic campylobacter spp. in dressed porcine carcass of chitwan, nepal. | campylobacter is the primary cause of food borne gastroenteritis. moreover, the emergence of multiple drug resistant campylobacters from poultry and pork has produced a potential threat to public health. research addressing these issues is sparse in nepal. so, this cross-sectional study aims at determining the prevalence, antibiogram and risk factors of campylobacters from dressed porcine carcass of chitwan, nepal. | 2014 | 24708489 |
| a gene-by-gene approach to bacterial population genomics: whole genome mlst of campylobacter. | campylobacteriosis remains a major human public health problem world-wide. genetic analyses of campylobacter isolates, and particularly molecular epidemiology, have been central to the study of this disease, particularly the characterization of campylobacter genotypes isolated from human infection, farm animals, and retail food. these studies have demonstrated that campylobacter populations are highly structured, with distinct genotypes associated with particular wild or domestic animal sources, ... | 2012 | 24704917 |
| molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections. | over the past few decades, nucleic acid-based methods have been developed for the diagnosis of intestinal parasitic infections. advantages of nucleic acid-based methods are numerous; typically, these include increased sensitivity and specificity and simpler standardization of diagnostic procedures. dna samples can also be stored and used for genetic characterization and molecular typing, providing a valuable tool for surveys and surveillance studies. a variety of technologies have been applied, ... | 2014 | 24696439 |
| prevalence of zoonotic campylobacter spp. in rectal swabs from dogs in slovakia: special reference to c. jejuni and c. coli. | this work focused on the isolation of thermophilic campylobacter spp. in samples obtained from dog droppings. there were 135 samples collected and examined from both clinically healthy and diseased dogs from households, clinics, rehabilitation centres and dog shelters in eastern slovakia. the isolation of the campylobacter spp. was achieved by the use of combined selective cultivation methods, followed by confirmation and species identification of the isolates using the pcr method.the overall pr ... | 2014 | 24693660 |
| cryptic ecology among host generalist campylobacter jejuni in domestic animals. | homologous recombination between bacterial strains is theoretically capable of preventing the separation of daughter clusters, and producing cohesive clouds of genotypes in sequence space. however, numerous barriers to recombination are known. barriers may be essential such as adaptive incompatibility, or ecological, which is associated with the opportunities for recombination in the natural habitat. campylobacter jejuni is a gut colonizer of numerous animal species and a major human enteric pat ... | 2014 | 24689900 |
| molecular mechanisms of quinolone, macrolide, and tetracycline resistance among campylobacter isolates from initial stages of broiler production. | the aim of this study was to investigate the resistance mechanisms of quinolones, macrolides and tetracycline in campylobacter isolates from grandparent and parent broiler breeders in spain. twenty-six isolates were investigated for quinolone resistance, three isolates for macrolide resistance and 39 for tetracycline resistance. all of the quinolone-resistant isolates possessed the mutation thr86ile in the quinolone resistance-determining region of gyra and one isolate possessed the mutation pro ... | 2014 | 24689432 |
| prevalence of virulence genes in strains of campylobacter jejuni isolated from human, bovine and broiler. | campylobacter jejuni isolates of different origins (bovine, broiler meat, human) were screened by polymerase chain reaction for the presence of 4 genes cdtb, cst-ii, ggt, and virb11, previously linked to virulence such as adherence, invasion, colonization, molecular mimicry, and cytotoxin production. in addition, the isolates were screened for the presence of the global gene regulator csra linked to oxidative stress responses, biofilms formation, and cell adhesion. all the c. jejuni isolates wer ... | 2014 | 24688515 |
| [bacterial biofilms: their importance in animal health and public health]. | bacterial biofilms are structured communities of bacterial cells enclosed in a self-produced polymer matrix that is attached to a surface. biofilms protect and allow bacteria to survive and thrive in hostile environments. bacteria within biofilms can withstand host immune responses, and are much less susceptible to antibiotics and disinfectants when compared to their planktonic counterparts. the ability to form biofilms is now considered an attribute of many microorganisms. diseases associated w ... | 2014 | 24688172 |
| a reference pan-genome approach to comparative bacterial genomics: identification of novel epidemiological markers in pathogenic campylobacter. | the increasing availability of hundreds of whole bacterial genomes provides opportunities for enhanced understanding of the genes and alleles responsible for clinically important phenotypes and how they evolved. however, it is a significant challenge to develop easy-to-use and scalable methods for characterizing these large and complex data and relating it to disease epidemiology. existing approaches typically focus on either homologous sequence variation in genes that are shared by all isolates ... | 2014 | 24676150 |
| alternative bacteriophage life cycles: the carrier state of campylobacter jejuni. | members of the genus campylobacter are frequently responsible for human enteric disease, often through consumption of contaminated poultry products. bacteriophages are viruses that have the potential to control pathogenic bacteria, but understanding their complex life cycles is key to their successful exploitation. treatment of campylobacter jejuni biofilms with bacteriophages led to the discovery that phages had established a relationship with their hosts typical of the carrier state life cycle ... | 2014 | 24671947 |
| evaluation and single-laboratory verification of a proposed modification to the u.s. food and drug administration method for detection and identification of campylobacter jejuni or campylobacter coli from raw silo milk. | the current u.s. food and drug administration (fda) methodology for detection of campylobacter, a leading source for foodborne illness, is outdated. the purpose of this study, therefore, was to improve and update the cultural and identification methods found in the fda/bacteriological analytical manual (bam). raw silo milk samples containing typical and atypical strains of campylobacter jejuni and campylobacter coli at different levels (5 cfu/25 g, 50 cfu/25 g, and 125 cfu/25 g) were analyzed. v ... | 2014 | 24645512 |
| high-throughput sequencing of campylobacter jejuni insertion mutant libraries reveals mapa as a fitness factor for chicken colonization. | campylobacter jejuni is a leading cause of gastrointestinal infections worldwide, due primarily to its ability to asymptomatically colonize the gastrointestinal tracts of agriculturally relevant animals, including chickens. infection often occurs following consumption of meat that was contaminated by c. jejuni during harvest. because of this, much interest lies in understanding the mechanisms that allow c. jejuni to colonize the chicken gastrointestinal tract. to address this, we generated a c. ... | 2014 | 24633877 |
| isolation of a campylobacter lanienae-like bacterium from laboratory chinchillas (chinchilla laniger). | routine necropsies of 27 asymptomatic juvenile chinchillas revealed a high prevalence of gastric ulcers with microscopic lymphoplasmacytic gastroenteritis and typhlocolitis. polymerase chain reaction (pcr) analysis using campylobacter genus-specific partial 16s rrna primers revealed the presence of campylobacter spp. dna in the faeces of 12 of 27 animals (44.4%). species-specific partial 16s rrna pcr and sequencing confirmed that these animals were colonized with campylobacter lanienae, a gram-n ... | 2014 | 24628887 |
| draft genome sequence of campylobacter coli strain ipsid-1 isolated from a patient with immunoproliferative small intestinal disease. | the genome sequence and annotation of campylobacter coli strain ipsid-1 are reported here. this bacterial isolate is the first to be cultured from a patient with immunoproliferative small intestinal disease (ipsid). the draft genome sequence is 1.683 mb long, comprises 64 contigs, and has 31.26% g+c content. | 2014 | 24625865 |
| campylobacter bacteremia: a rare and under-reported event? | bacteria belonging to the species campylobacter are the most common cause of bacterial diarrhoea in humans. the clinical phenotype associated with campylobacter infections ranges from asymptomatic conditions to severe colitis and bacteremia. in susceptible patients, campylobacter infections are associated with significant morbidity and mortality, with both host factors and bacterial factors being involved in the pathogenesis of bacteremia. in the host, age, gender and immune-compromising conditi ... | 2012 | 24611124 |
| validation according to iso 16140:2003 of a commercial real-time pcr-based method for detecting campylobacter jejuni, c. coli, and c. lari in foods. | campylobacteriosis was the most frequently reported zoonosis in the european union (eu) in 2010, with campylobacter jejuni, campylobacter coli, and campylobacter lari as the most frequently reported species in foodborne outbreaks (fbos). relatively sensitive to environmental factors, these species may be present in low numbers. in line with eu policy for food control and fbo detection and in view of the need to reduce response time, we validated an alternative molecular method according to iso 1 ... | 2014 | 24607425 |