Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| occurrence of campylobacter in retail foods in ireland. | a surveillance study was carried out to determine the prevalence of campylobacter in a range of retail foods purchased in three irish cities over a 20-month period between march 2001 and october 2002. in total 2391 food samples were analysed during this period. campylobacter was isolated from 444 raw chicken (49.9%), 33 turkey (37.5%) and 11 duck samples (45.8%). lower isolation rates of 7/221 (3.2%), 10/197 (5.1%) and 31/262 (11.8%) were observed for raw beef, pork and lamb, respectively. one s ... | 2004 | 15282123 |
| longitudinal study of the excretion patterns of thermophilic campylobacter spp. in young pet dogs in denmark. | the campylobacter excretion patterns of 26 domestic pet dogs were described in a longitudinal study. the dogs entered the study between 3 and 8 months of age and were monitored until 2 years of age. they were tested monthly for campylobacter carriage in stool samples that were cultured on the campylobacter-selective media cat and modified ccda agar at 37 and 42 degrees c. this study comprised 366 fecal swab samples, of which 278 (76.2%) were found to be campylobacter positive, with the following ... | 2004 | 15131162 |
| helicobacter pylori flgr is an enhancer-independent activator of sigma54-rna polymerase holoenzyme. | helicobacter pylori flgr activates transcription with sigma54-rna polymerase holoenzyme (sigma54-holoenzyme) from at least five flagellar operons. activators of sigma54-holoenzyme generally bind enhancer sequences located >70 bp upstream of the promoter and contact sigma54-holoenzyme bound at the promoter through dna looping to activate transcription. h. pylori flgr lacks the carboxy-terminal dna-binding domain present in most sigma54-dependent activators. as little as 42 bp of dna upstream of t ... | 2004 | 15231786 |
| the pattern and kinetics of substrate metabolism of campylobacter jejuni and campylobacter coli. | the main aim was to investigate the patterns and kinetics of substrate oxidation by campylobacter jejuni and c. coli. | 2004 | 15287872 |
| role of an inducible single-domain hemoglobin in mediating resistance to nitric oxide and nitrosative stress in campylobacter jejuni and campylobacter coli. | campylobacter jejuni expresses two hemoglobins, each of which exhibits a heme pocket and structural signatures in common with vertebrate and plant globins. one of these, designated cgb, is homologous to vgb from vitreoscilla stercoraria and does not possess the reductase domain seen in the flavohemoglobins. a cgb-deficient mutant of c. jejuni was hypersensitive to nitrosating agents (s-nitrosoglutathione [gsno] or sodium nitroprusside) and a nitric oxide-releasing compound (spermine nonoate). th ... | 2004 | 15292134 |
| antimicrobial susceptibilities of campylobacter isolated from food-producing animals on farms (1999-2001): results from the japanese veterinary antimicrobial resistance monitoring program. | nationwide monitoring of antimicrobial resistance in campylobacter isolated from food-producing animals on farms was performed in japan. a total of 468 campylobacter isolates were obtained during the period from june 1999 to march 2001. campylobacter species showed high frequencies of resistance to oxytetracycline and dihydrostreptomycin. the frequencies of resistance in campylobacter coli to aminoglycosides, macrolides, tetracycline and quinolones were higher than those in campylobacter jejuni ... | 2004 | 15325430 |
| prevalence and antimicrobial resistance of campylobacter coli isolated from fattening pigs in france. | campylobacter are a leading cause of human diarrhea. the usual source of infection is contaminated food, particularly poultry but pork has also been described. the veterinary use of antimicrobial drugs has been suggested to be largely responsible for resistance in human isolates of this zoonotic pathogen. a study was carried out to investigate the occurrence and antimicrobial resistance of campylobacter isolated from french fattening pigs. from march 1998 to june 1999, stomach samples were colle ... | 2004 | 15172691 |
| antigenicity of the campylobacter coli cjaa protein produced by escherichia coli. | immunogenic c. coli cjaa protein is a candidate for a chicken anti-campylobacter subunit vaccine. in order to enhance its immunogenicity an antigenic determinant of the cjaa protein was identified. thereafter, two copies of the antigenic epitope were cloned in tandem directly or with a flexible hinge between them. all experiments documented that the cjaa protein contained not only linear antigenic epitope/s but also conformational ones. | 2004 | 15330269 |
| use of midi-fatty acid methyl ester analysis to monitor the transmission of campylobacter during commercial poultry processing. | the presence of campylobacter spp. on broiler carcasses and in scald water taken from a commercial poultry processing facility was monitored on a monthly basis from january through june. campylobacter agar, blaser, was used to enumerate campylobacter in water samples from a multiple-tank scalder; on prescalded, picked, eviscerated, and chilled carcasses; and on processed carcasses stored at 4 degrees c for 7 or 14 days. the midi sherlock microbial identification system was used to identify campy ... | 2004 | 15330523 |
| use of culture, pcr analysis, and dna microarrays for detection of campylobacter jejuni and campylobacter coli from chicken feces. | a dna microarray for detection of campylobacter spp. was recently developed and applied to detect campylobacter spp. directly from chicken feces. sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. the results of campylobacter sp. detection obtained with dna microarrays were compared to those obtained by conventional culture and gel electrophoresis. by conventional culture, 60% of the samples were positive for either campylobacter jejun ... | 2004 | 15364980 |
| campylobacter, from obscurity to celebrity. | after its successful isolation from stools in the 1970s, campylobacter jejuni has rapidly become the most commonly recognised cause of bacterial gastroenteritis in man. reported cases of human campylobacteriosis represent only a small fraction of the actual number. in industrialised countries, the incidence of c. jejuni/campylobacter coli infections peaks during infancy, and again in young adults aged 15-44 years. acute self-limited gastrointestinal illness, characterised by diarrhoea, fever and ... | 2004 | 15373879 |
| a european survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing animals. | to study antimicrobial resistance in zoonotic bacteria isolated from food animals in different countries using uniform methodology. | 2004 | 15375107 |
| nucleotide sequences and comparison of two large conjugative plasmids from different campylobacter species. | two large tetracycline resistance (tcr) plasmids have been completely sequenced, the ptet plasmid (45.2 kb) from campylobacter jejuni strain 81-176 and a plasmid pcc31 (44.7 kb) from campylobacter coli strain cc31 that was isolated from a human case of severe gastroenteritis in the uk. both plasmids are mosaic in structure, having homologues of genes found in a variety of different commensal and pathogenic bacteria, but nevertheless, showed striking similarities in dna sequence and overall gene ... | 2004 | 15470128 |
| swimming and campylobacter infections. | a matched case-control study was conducted to study risk factors for domestically acquired sporadic campylobacter infections in finland. swimming in natural sources of water was a novel risk factor. eating undercooked meat and drinking dug-well water were also independent risk factors for campylobacter infection. | 2004 | 15496253 |
| antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates in japan. | 2004 | 15499812 | |
| sensitive and specific detection of yersinia pseudotuberculosis by loop-mediated isothermal amplification. | we developed a loop-mediated isothermal amplification method able to detect yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. this method is more sensitive than pcr and might be a useful tool for detecting and identifying y. pseudotuberculosis. | 2004 | 15528740 |
| insufficient production and tissue delivery of cd4+ memory t cells in rapidly progressive simian immunodeficiency virus infection. | the mechanisms linking human immunodeficiency virus replication to the progressive immunodeficiency of acquired immune deficiency syndrome are controversial, particularly the relative contribution of cd4+ t cell destruction. here, we used the simian immunodeficiency virus (siv) model to investigate the relationship between systemic cd4+ t cell dynamics and rapid disease progression. of 18 rhesus macaques (rms) infected with ccr5-tropic sivmac239 (n=14) or cxcr4-tropic sivmac155t3 (n=4), 4 of the ... | 2004 | 15545355 |
| campylobacter insulaenigrae sp. nov., isolated from marine mammals. | phenotypic and phylogenetic studies were performed on four campylobacter-like organisms recovered from three seals and a porpoise. comparative 16s rrna gene sequencing studies demonstrated that the organisms represent a hitherto unknown subline within the genus campylobacter, associated with a subcluster containing campylobacter jejuni, campylobacter coli and campylobacter lari. dna-dna hybridization studies confirmed that the bacteria belonged to a single species, for which the name campylobact ... | 2004 | 15545485 |
| pcr-restriction fragment length polymorphism assay for detection of gyra mutations associated with fluoroquinolone resistance in campylobacter coli. | a fragment of the gyra gene was sequenced from 34 isolates of campylobacter coli, including 23 isolates resistant to ciprofloxacin. all ciprofloxacin-resistant isolates examined by dna sequencing carried a point mutation at position thr-86 on the gyra gene product, involving the replacement of thr-86 by ile. a combined pcr-restriction fragment length polymorphism technique using rsai was developed to detect this mutation. | 2004 | 15561873 |
| campylobacter colonization of sibling turkey flocks reared under different management conditions. | uncertainty exists concerning the key factors contributing to campylobacter colonization of poultry, especially the possible role of vertical transmission from breeder hens to young birds. a longitudinal study of campylobacter colonization was performed in two sibling pairs of turkey flocks (four flocks total). each pair of sibling flocks shared breeder hen populations and was obtained from the same hatchery. one flock of each pair was grown on a commercial farm, and the other was grown in an in ... | 2004 | 15270502 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| diagnostic real-time pcr for detection of salmonella in food. | a robust 5' nuclease (taqman) real-time pcr was developed and validated in-house for the specific detection of salmonella in food. the assay used specifically designed primers and a probe target within the ttrrsbca locus, which is located near the salmonella pathogenicity island 2 at centisome 30.5. it is required for tetrathionate respiration in salmonella. the assay correctly identified all 110 salmonella strains and 87 non-salmonella strains tested. an internal amplification control, which is ... | 2004 | 15574899 |
| characterization of lactobacillus coryniformis dsm 20001t surface protein cpf mediating coaggregation with and aggregation among pathogens. | phenotypic characterization of aggregation phenotypes of lactobacillus coryniformis revealed that strain dsm 20001t coaggregated with escherichia coli k88, campylobacter coli, and campylobacter jejuni but not with other human pathogens. in addition, cells of these pathogens aggregated in the presence of the spent culture supernatant (scs) of strain dsm 20001t. cells of e. coli k88 remained viable in the coaggregates and aggregates for up to 24 h. both coaggregation and aggregation (co/aggregatio ... | 2004 | 15574903 |
| survival of cold-stressed campylobacter jejuni on ground chicken and chicken skin during frozen storage. | campylobacter jejuni is prevalent in poultry, but the effect of combined refrigerated and frozen storage on its survival, conditions relevant to poultry processing and storage, has not been evaluated. therefore, the effects of refrigeration at 4 degrees c, freezing at -20 degrees c, and a combination of refrigeration and freezing on the survival of c. jejuni in ground chicken and on chicken skin were examined. samples were enumerated using tryptic soy agar containing sheep's blood and modified c ... | 2004 | 15574906 |
| the neuc protein of escherichia coli k1 is a udp n-acetylglucosamine 2-epimerase. | the k1 capsule is an essential virulence determinant of escherichia coli strains that cause meningitis in neonates. biosynthesis and transport of the capsule, an alpha-2,8-linked polymer of sialic acid, are encoded by the 17-kb kps gene cluster. we deleted neuc, a k1 gene implicated in sialic acid synthesis, from the chromosome of ev36, a k-12-k1 hybrid, by allelic exchange. exogenously added sialic acid restored capsule expression to the deletion strain (deltaneuc), confirming that neuc is nece ... | 2004 | 14729696 |
| gastroenteritis in nf-kappab-deficient mice is produced with wild-type camplyobacter jejuni but not with c. jejuni lacking cytolethal distending toxin despite persistent colonization with both strains. | campylobacter jejuni continues to be a leading cause of bacterial enteritis in humans. however, because there are no readily available animal models to study the pathogenesis of c. jejuni-related diseases, the significance of potential virulence factors, such as cytolethal distending toxin (cdt), in vivo are poorly understood. mice deficient in nf-kappab subunits (p50(-/-) p65(+/-)) in a c57bl/129 background are particularly susceptible to colitis induced by another enterohepatic microaerobe, he ... | 2004 | 14742559 |
| proteomic analysis of the sarcosine-insoluble outer membrane fraction of helicobacter pylori strain 26695. | helicobacter pylori causes gastroduodenal disease, which is mediated in part by its outer membrane proteins (omps). to identify omps of h. pylori strain 26695, we performed a proteomic analysis. a sarcosine-insoluble outer membrane fraction was resolved by two-dimensional electrophoresis with immobilized ph gradient strips. most of the protein spots, with molecular masses of 10 to 100 kda, were visible on the gel in the alkaline pi regions (6.0 to 10.0). the proteome of the omps was analyzed by ... | 2004 | 14761989 |
| genotypic and antibiotic susceptibility characteristics of a campylobacter coli population isolated from dairy farmland in the united kingdom. | campylobacter infections are the most common cause of bacterial enteritis in humans, and nearly 8% of such infections are caused by campylobacter coli. most studies have concentrated on campylobacter jejuni, frequently isolated from intensively farmed poultry and livestock production units, and few studies have examined the spread and relatedness of campylobacter across a range of geographical and host boundaries. systematic sampling of a 100-km2 area of mixed farmland in northwest england yield ... | 2004 | 14766560 |
| campylobacter spp., giardia spp., cryptosporidium spp., noroviruses, and indicator organisms in surface water in southwestern finland, 2000-2001. | a total of 139 surface water samples from seven lakes and 15 rivers in southwestern finland were analyzed during five consecutive seasons from autumn 2000 to autumn 2001 for the presence of various enteropathogens (campylobacter spp., giardia spp., cryptosporidium spp., and noroviruses) and fecal indicators (thermotolerant coliforms, escherichia coli, clostridium perfringens, and f-rna bacteriophages) and for physicochemical parameters (turbidity and temperature); this was the first such systema ... | 2004 | 14711629 |
| fluorescent amplified fragment length polymorphism genotyping of campylobacter jejuni and campylobacter coli strains and its relationship with host specificity, serotyping, and phage typing. | fluorescent amplified fragment length polymorphism (faflp) analysis was applied to 276 campylobacter jejuni strains and 87 campylobacter coli strains isolated from humans, pigs, cattle, poultry, and retail meats to investigate whether certain faflp genotypes of c. jejuni and c. coli are associated with a particular host and to determine the degree of association between faflp-defined genotypes and heat-stable serotypes and/or phage types. within c. coli, the poultry strains clustered separately ... | 2004 | 14715757 |
| comparison of survival of campylobacter jejuni in the phyllosphere with that in the rhizosphere of spinach and radish plants. | campylobacter jejuni has been isolated previously from market produce and has caused gastroenteritis outbreaks linked to produce. we have tested the ability of this human pathogen to utilize organic compounds that are present in leaf and root exudates and to survive in the plant environment under various conditions. carbon utilization profiles revealed that c. jejuni can utilize many organic acids and amino acids available on leaves and roots. despite the presence of suitable substrates in the p ... | 2004 | 14766604 |
| simultaneous presence of multiple campylobacter species in dogs. | the prevalence of coinfection of campylobacter species in dogs was determined using four isolation methods. in 26% of the positive-testing stools, multiple campylobacter species were identified. the use of multiple isolation methods as well as the time lapse between sampling and processing are important for detection of coinfection. | 2004 | 14766860 |
| l-serine catabolism via an oxygen-labile l-serine dehydratase is essential for colonization of the avian gut by campylobacter jejuni. | campylobacter jejuni is a microaerophilic, asaccharolytic bacterium. the identity of the carbon and energy sources used by c. jejuni in vivo is unknown, but the genome sequence of strain nctc11168 indicates the presence of genes for catabolism of a limited range of amino acids, including serine. specific omission of l-serine from a defined medium containing a mixture of amino acids led to a dramatic decrease in cell yields. as c. jejuni does not have a biosynthetic serine requirement, this suppo ... | 2004 | 14688104 |
| phase and antigenic variation in bacteria. | phase and antigenic variation result in a heterogenic phenotype of a clonal bacterial population, in which individual cells either express the phase-variable protein(s) or not, or express one of multiple antigenic forms of the protein, respectively. this form of regulation has been identified mainly, but by no means exclusively, for a wide variety of surface structures in animal pathogens and is implicated as a virulence strategy. this review provides an overview of the many bacterial proteins a ... | 2004 | 15258095 |
| [incidence of the virulence markers iam in campylobacter jejuni and campylobacter coli strains isolated from poultry carcases]. | previously found dna sequence (iam), correlated with the invasiveness of campylobacter strains, became a starting point for present investigation. main goal of this study was to isolate number of campylobacter strains from chicken carcasses, to determine their taxonomic position and to establish the presence of iam sequence in their genoms. it was found, that invasion associated marker is present in all campylobacter coli strains and in majority but not all campylobacter jejuni. this may confirm ... | 2004 | 19097580 |
| comparison of electrolyzed oxidizing water with other antimicrobial interventions to reduce pathogens on fresh pork. | to date, the effectiveness of electrolyzed oxidizing (eo) water against bacteria associated with fresh pork has not been determined. using a hand-held, food-grade garden sprayer, distilled water (w), chlorinated water (cl; 25 ppm), 2% lactic acid (la), acidic eo water (eoa), or "aged" acidic eo water (aeoa; stored at 4 °c for 24 h) was sprayed (15 s) onto pork bellies inoculated with feces containing listeria monocytogenes (lm), salmonella typhimurium (st), and campylobacter coli (cc). remaining ... | 2004 | 22062415 |
| identification and molecular epidemiology of campylobacter coli isolates from human gastroenteritis, food, and animal sources by amplified fragment length polymorphism analysis and penner serotyping. | campylobacter coli is an infrequently studied but important food-borne pathogen with a wide natural distribution. we investigated its molecular epidemiology by use of amplified fragment length polymorphism (aflp)-based genotyping and penner serotyping. serotype reference strains and 177 danish isolates of diverse origin identified by routine phenotyping as c. coli were examined. molecular tools identified some 12% of field isolates as campylobacter jejuni, emphasizing the need for improved ident ... | 2005 | 15812025 |
| mama-pcr assay for the detection of point mutations associated with high-level erythromycin resistance in campylobacter jejuni and campylobacter coli strains. | twenty campylobacter jejuni and 16 campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. a combined mismatch amplification mutation assay-pcr technique was developed to detect the mutations a 2074 c and a 2075 g in the 23s rrna gene associated with erythromycin resistance. all high-level erythromycin-resistant strains examined by dna sequencing carried the transition mutation a 2075 g, whereas no isolate carried the a 20 ... | 2005 | 15927294 |
| identification and characterization of neub3 from campylobacter jejuni as a pseudaminic acid synthase. | campylobacter jejuni and campylobacter coli are the main causes of bacterial diarrhea worldwide, and helicobacter pylori is known to cause duodenal ulcers. in all of these pathogenic organisms, the flagellin proteins are heavily glycosylated with a 2-keto-3-deoxy acid, pseudaminic acid (5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-nonulosonic acid). the presence of pseudaminic acid is required for the proper development of the flagella and is thereby necessary for motility in, and invasi ... | 2005 | 16120604 |
| occurrence of campylobacter species in poultry forms in lagos area of nigeria. | samples of poultry droppings were collected from fifty poultry farms in lagos area of nigeria. campylobacter species were isolated from serially diluted samples using bolton selective enrichment medium under microaerophilic condition at 42 degrees c. samples of water, both treated and untreated used in the farms were also analysed in the same manner. fifteen campylobacter strains were isolated from the droppings. all the strains were identified using biochemical characteristics differentiating c ... | 2005 | 16334275 |
| campylobacter in food animals and humans in northern thailand. | cross-sectional, longitudinal, and case-control studies were conducted to describe the epidemiology of campylobacter in chickens, swine, dairy cows, farm workers, nonfarm residents, and children with diarrhea. samples were collected in chiang mai and lamphung provinces of northern thailand from 2000 through 2003. a total of 2,360 samples were processed. results from the cross-sectional study indicated that the prevalences of campylobacter in chickens at the farm, slaughterhouse, and market were ... | 2005 | 16355821 |
| characterization of a cmeabc operon in a quinolone-resistant campylobacter coli isolate of irish origin. | in this study, a tripartite-operon-encoding efflux system together with its regulatory gene was characterized in an irish campylobacter coli isolate cit-382 showing high-level resistance to nalidixic acid and ciprofloxacin. sequence comparisons revealed significant homology between c. coli and the cmeabc operon of campylobacter jejuni. conservation of functional sequence domains and motifs were noted among c. coli and similar operons in unrelated organisms. a transcriptional regulatory gene cmer ... | 2005 | 16359189 |
| campylobacter coli: prevalence and antimicrobial resistance in antimicrobial-free (abf) swine production systems. | to determine the prevalence and antimicrobial resistance of campylobacter species in swine reared in the intensive and extensive antimicrobial-free (abf) production systems at farm and slaughter. in the abf system, antimicrobials are neither used for growth promotion nor therapeutic purposes. | 2005 | 16120624 |
| involvement of the cmeabc efflux pump in the macrolide resistance of campylobacter coli. | this study was conducted to examine the role of the cmeabc efflux pump in decreasing the susceptibility of campylobacter coli to macrolides and ketolides in the context of absence or presence of mutations in the 23s rrna genes. | 2005 | 16157618 |
| factors affecting the pressure resistance of some campylobacter species. | to compare pressure resistance between strains of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter fetus, and to investigate the effect of suspending medium on pressure resistance of sensitive and more resistant strains. | 2005 | 16162138 |
| mortality following campylobacter infection: a registry-based linkage study. | campylobacteriosis is one of the most commonly identified causes of bacterial diarrheal disease and a common cause of gastroenteritis in travellers from developed nations. despite the widespread occurrence, there is little information on campylobacter mortality. | 2005 | 16162289 |
| acid-induced activation of the urease promoters is mediated directly by the arsrs two-component system of helicobacter pylori. | the nickel-containing enzyme urease is an essential colonization factor of the human gastric pathogen helicobacter pylori which enables the bacteria to survive the low-ph conditions of the stomach. transcription of the urease genes is positively controlled in response to increasing concentrations of nickel ions and acidic ph. here we demonstrate that acid-induced transcription of the urease genes is mediated directly by the arsrs two-component system. footprint analyses identify binding sites of ... | 2005 | 16177315 |
| campylobacter coli in swine production: antimicrobial resistance mechanisms and molecular epidemiology. | the aim of this study was to determine antimicrobial resistance, to evaluate and compare the use of two genotyping methods for molecular epidemiology purposes, and to determine the genotypic diversity of campylobacter coli of porcine origin. a total of 100 c. coli isolates from swine were tested for susceptibility to six antimicrobials using the agar dilution method and genotyped using two high-resolution fingerprinting approaches: multilocus sequence typing (mlst) and pulsed-field gel electroph ... | 2005 | 16272508 |
| microbial health risk posed by table eggs in trinidad. | a survey of the microbial quality of table eggs sold in trinidad was conducted. for 23 poultry layer farms each visited twice approximately 1 month apart, 25 pooled eggs constituted a composite sample, for 14 shopping malls each visited twice approximately 1 month apart, six pooled eggs made a composite sample and for a total of 102 other retailers across the country each visited once over a 4-month period, six pooled eggs constituted a composite sample. swabs of egg shells and egg content were ... | 2005 | 16274501 |
| multiplex pcr for identification of campylobacter coli and campylobacter jejuni from pure cultures and directly on stool samples. | a multiplex-pcr method, specifically designed for application in routine diagnostic laboratories, was developed for the detection of campylobacter coli and campylobacter jejuni. primers were directed towards the following loci: the hippuricase gene (hipo) characteristic of c. jejuni, a sequence partly covering an aspartokinase gene characteristic of c. coli, and a universal 16s rdna gene sequence serving as an internal positive control for the pcr. the method was tested on 47 c. coli strains and ... | 2005 | 16192435 |
| quantification of campylobacter spp. in chicken rinse samples by using flotation prior to real-time pcr. | real-time pcr is fast, sensitive, specific, and can deliver quantitative data; however, two disadvantages are that this technology is sensitive to inhibition by food and that it does not distinguish between dna originating from viable, viable nonculturable (vnc), and dead cells. for this reason, real-time pcr has been combined with a novel discontinuous buoyant density gradient method, called flotation, in order to allow detection of only viable and vnc cells of thermotolerant campylobacters in ... | 2005 | 16204485 |
| prevalence and antimicrobial resistance of campylobacter in antimicrobial-free and conventional pig production systems. | the objectives of this study were to determine and compare the prevalence and antimicrobial resistance of campylobacter species in swine reared in conventional and antimicrobial-free (abf) production systems. campylobacter coli was the predominant species, with 1,459 isolates (99%) in the study. we found significantly higher prevalence of c. coli on the abf farms (77.3%) than on the conventional farms (27.6%) among pigs at the nursery stage (p < 0.001). at slaughter, we found significantly highe ... | 2005 | 16300080 |
| quantifying transmission of campylobacter spp. among broilers. | campylobacter species are frequently identified as a cause of human gastroenteritis, often from eating or mishandling contaminated poultry products. quantitative knowledge of transmission of campylobacter in broiler flocks is necessary, as this may help to determine the moment of introduction of campylobacter in broiler flocks more precisely. the aim of this study was to determine the transmission rate parameter in broiler flocks. four experiments were performed, each with four campylobacter-ino ... | 2005 | 16204486 |
| genomic diversity of campylobacter coli and campylobacter jejuni isolates recovered from free-range broiler farms and comparison with isolates of various origins. | in many industrialized countries, the incidence of campylobacteriosis exceeds that of salmonellosis. campylobacter bacteria are transmitted to humans mainly in food, especially poultry meat products. total prevention of campylobacter colonization in broiler flocks is the best way to reduce (or eliminate) the contamination of poultry products. the aim of this study was to establish the sources and routes of contamination of broilers at the farm level. molecular typing methods (dna macrorestrictio ... | 2005 | 16204541 |
| origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new members. | we report an in-depth computational study of the protein sequences and structures of the superfamily of archaeo-eukaryotic primases (aeps). this analysis greatly expands the range of diversity of the aeps and reveals the unique active site shared by all members of this superfamily. in particular, it is shown that eukaryotic nucleo-cytoplasmic large dna viruses, including poxviruses, asfarviruses, iridoviruses, phycodnaviruses and the mimivirus, encode aeps of a distinct family, which also includ ... | 2005 | 16027112 |
| speciation of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | multiple strains of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis isolated from animal, clinical, or food samples have been analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). whole bacterial cells were harvested from colonies or confluent growth on agar and transferred directly into solvent and then to a spot of dried 3-methoxy-4-hydroxycinnamic acid (matrix). multiple ions in the 5,000- to 15,000-da m ... | 2005 | 16204551 |
| differentiation of campylobacter populations as demonstrated by flagellin short variable region sequences. | the dna sequence of the flaa short variable region (svr) was used to analyze a random population of campylobacter isolates to investigate the weakly clonal population structure of members of the genus. the svr sequence from 197 strains of c. jejuni and c. coli isolated from humans, bovine, swine, and chickens identified a group of 43 strains containing disparate short variable region sequences compared to the rest of the population. this group contains both c. jejuni and c. coli strains but disp ... | 2005 | 16204559 |
| delineation of campylobacter concisus genomospecies by amplified fragment length polymorphism analysis and correlation of results with clinical data. | campylobacter concisus has been as frequently isolated from human diarrhea as the important enteropathogen campylobacter jejuni, but it also occurs in the feces of healthy individuals. the role of c. concisus in human disease has been difficult to determine, since the species comprises at least two phenotypically indistinguishable but genetically distinct taxa (i.e., genomospecies) that may vary in pathogenicity. we examined 62 c. concisus strains by amplified fragment length polymorphism (aflp) ... | 2005 | 16207968 |
| comparative phylogenomics of the food-borne pathogen campylobacter jejuni reveals genetic markers predictive of infection source. | campylobacter jejuni is the predominant cause of bacterial gastroenteritis worldwide, but traditional typing methods are unable to discriminate strains from different sources that cause disease in humans. we report the use of genomotyping (whole-genome comparisons of microbes using dna microarrays) combined with bayesian-based algorithms to model the phylogeny of this major food-borne pathogen. in this study 111 c. jejuni strains were examined by genomotyping isolates from humans with a spectrum ... | 2005 | 16230626 |
| the heterogeneity of campylobacter jejuni and campylobacter coli strains isolated from healthy cattle. | to identify campylobacters isolated from clinically healthy cattle at species level by a multiplex polymerase chain reaction (m-pcr). the heterogeneity among campylobacter jejuni and campylobacter coli isolates was also investigated by using a restriction fragment length polymorphism (rflp) analysis of flagellin (flaa) gene. | 2005 | 16238642 |
| dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks. | bacterial response to nitric oxide (no) is of major importance since no is an obligatory intermediate of the nitrogen cycle. transcriptional regulation of the dissimilatory nitric oxides metabolism in bacteria is diverse and involves fnr-like transcription factors hcpr, dnr, and nnrr; two-component systems narxl and narqp; no-responsive activator norr; and nitrite-sensitive repressor nsrr. using comparative genomics approaches, we predict dna-binding motifs for these transcriptional factors and ... | 2005 | 16261196 |
| the importance of campylobacter coli in human campylobacteriosis: prevalence and genetic characterization. | the primary aim of this study was to detect and genotype campylobacter coli strains from humans over a period of one year from november 2002 to october 2003. campylobacter spp. were isolated from patients with symptoms of enteric infection. amplified fragment length polymorphism (aflp) analysis was used to identify the genetic diversity of c. coli strains by cluster analysis. a total of 18.6% of all campylobacter isolates were identified as c. coli. these data show, that c. coli is indeed of imp ... | 2005 | 16274505 |
| a new approach for the analysis of bacterial microarray-based comparative genomic hybridization: insights from an empirical study. | microarray-based comparative genomic hybridization (m-cgh) has been used to characterize the extensive intraspecies genetic diversity found in bacteria at the whole-genome level. although conventional microarray analytical procedures have proved adequate in handling m-cgh data, data interpretation using these methods is based on a continuous character model in which gene divergence and gene absence form a spectrum of decreasing gene conservation levels. however, whereas gene divergence may yet b ... | 2005 | 15918914 |
| cytolethal distending toxin (cdt)-negative campylobacter jejuni strains and anti-cdt neutralizing antibodies are induced during human infection but not during colonization in chickens. | the cytolethal distending toxin (cdt) of campylobacter jejuni was detectable, using an in vitro assay, in most but not all of 24 strains tested. the reason for the absence of toxin activity in these naturally occurring cdt-negative c. jejuni strains was then investigated at the genetic level. cdt is encoded by three highly conserved genes, cdta, -b, and -c. in the cdt-negative strains, two types of mutation were identified. the cdt activities of c. jejuni strains possessing both types of mutatio ... | 2005 | 15845513 |
| evaluation of a pcr assay for the detection and identification of campylobacter jejuni and campylobacter coli in retail poultry products. | a pcr-based method was applied to campylobacter detection in poultry samples at the retail level. in total, 73 retail poultry samples purchased from supermarkets in the basque country area in the north of spain were examined using both culture and molecular (alternative) methods. in our routine method, the worldwide iso 10272:1995 standard of preston broth incubated at 42 degrees c for conventional campylobacter detection was adopted. the molecular method was comprised of a dna extraction kit co ... | 2005 | 15862456 |
| crga is an inducible lysr-type regulator of neisseria meningitidis, acting both as a repressor and as an activator of gene transcription. | the crga gene of neisseria meningitidis, which codes for a lysr-type regulator, is divergently oriented with respect to the mdab gene, which codes for a hypothetical nadph-quinone oxidoreductase. transcriptional studies of the intergenic region between crga and mdab showed that two overlapping and divergent promoters, p(crga) and p(mdab), control transcription of these genes. deletion of the crga gene led to a strong increase in transcription from the p(crga) promoter and a concomitant strong de ... | 2005 | 15866928 |
| extended multilocus sequence typing system for campylobacter coli, c. lari, c. upsaliensis, and c. helveticus. | a multilocus sequence typing (mlst) system has been reported previously for campylobacter jejuni to both differentiate strains and identify clonal lineages. however, sequence variation at the mlst loci prevents its use for closely related campylobacter species. we describe herein an expanded mlst method to include three clinically relevant campylobacter species, c. coli, c. lari, and c. upsaliensis, and a fourth campylobacter species, c. helveticus. the c. coli and c. helveticus methods use the ... | 2005 | 15872261 |
| oral campylobacter species involved in extraoral abscess: a report of three cases. | oral campylobacter species are rarely reported to cause extraoral infections. here we present three cases of extraoral abscess caused by an oral campylobacter sp. and a streptococcus sp. the campylobacter species were all isolated anaerobically and identified by sequencing analysis of the 16s rrna gene. the cases included a breast abscess caused by campylobacter rectus and a non-group a beta-hemolytic streptococcus in a patient with lymphoma, a liver abscess caused by campylobacter curvus and an ... | 2005 | 15872299 |
| effect of preslaughter events on prevalence of campylobacter jejuni and campylobacter coli in market-weight turkeys. | the effects of events which occur prior to slaughter, such as loading, transport, and holding at an abattoir, on the prevalence of campylobacter species, including campylobacter jejuni and campylobacter coli, were examined. cloacal swabs from market-weight turkeys in each of five flocks were obtained on a farm prior to loading (time 1; 120 swabs per flock) and after transport and holding at the abattoir (time 2; 120 swabs per flock). a statistically significant increase in the overall prevalence ... | 2005 | 15932973 |
| h-ns is a part of a thermally controlled mechanism for bacterial gene regulation. | temperature is a primary environmental stress to which micro-organisms must be able to adapt and respond rapidly. whereas some bacteria are restricted to specific niches and have limited abilities to survive changes in their environment, others, such as members of the enterobacteriaceae, can withstand wide fluctuations in temperature. in addition to regulating cellular physiology, pathogenic bacteria use temperature as a cue for activating virulence gene expression. this work confirms that the n ... | 2005 | 15966862 |
| evaluation of agar plates for direct enumeration of campylobacter spp. from poultry carcass rinses. | campy-cefex, a modification of campy-cefex, modified charcoal cefoperazone deoxycholate (mccda), karmali, campy, and campy-line agars were evaluated for their efficiency to isolate and enumerate campylobacter spp. from poultry carcass rinses. campy-cefex and its modification produced the best results but were statistically similar to campy, mccda, and karmali. | 2005 | 15933040 |
| prevalence of potential virulence markers in polish campylobacter jejuni and campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. | the pathogenicity of thermotolerant campylobacter species, common food-borne pathogens, depends on certain factors unevenly distributed among strains of different origin. the prevalence of such markers has never been examined in a population of polish campylobacter strains of human and poultry origin. therefore, we analysed the presence of the cadf, cdta, cdtb and cdtc genes and the iam sequence in campylobacter jejuni (n = 115) and campylobacter coli (n = 57) isolates from children with diarrho ... | 2005 | 15947425 |
| function of neisserial outer membrane phospholipase a in autolysis and assessment of its vaccine potential. | outer membrane phospholipase a (ompla) is an outer membrane-localized enzyme, present in many gram-negative bacterial species. it is implicated in the virulence of several pathogens. here, we investigated the presence, function, and vaccine potential of ompla in the human pathogen neisseria meningitidis. immunoblot analysis showed the presence of ompla in 28 out of 33 meningococcal strains investigated. the ompla-negative strains all contained a plda gene, but these alleles contained premature s ... | 2005 | 15784566 |
| clinical herd health, farm management and antimicrobial resistance in campylobacter coli on finishing pig farms in switzerland. | the world-wide increase of antimicrobial resistance in micro-organisms complicates medical treatment of infected humans. we did a risk-factor analysis for the prevalence of antimicrobial resistant campylobacter coli on 64 swiss pig finishing farms. between may and november 2001, 20 faecal samples per farm were collected from the floor of pens holding finishing pigs shortly before slaughter. samples were pooled and cultured for campylobacter species. isolated campylobacter strains were tested for ... | 2005 | 15907569 |
| analysis of the prevalence of tetracycline resistance genes in clinical isolates of enterococcus faecalis and enterococcus faecium in a japanese hospital. | prevalence of seven tetracycline resistance (tc(r)) genes--tet(l), tet(m), tet(k), tet(o), tet(s), tet(t), and tet(u)--which are known to be distributed to gram-positive cocci was analyzed for 224 enterococcus faecalis and 46 enterococcus faecium clinical isolates obtained in a japanese hospital. any of the tc(r) genes was detected in 75.9% of all the enterococcal strains. the tet(m) was detected at highest rates in both e. faecalis (75.0%) and e. faecium (69.6%), followed by tet(l), which was h ... | 2005 | 15910229 |
| pcr identification of campylobacter coli and campylobacter jejuni by partial sequencing of virulence genes. | the objective of this study was to utilize a multiplex pcr assay for concurrent detection of campylobacter spp. and c. coli or c. jejuni, using probes derived from genes cadf and ceue and an undefined virulence gene. a total of 97 campylobacter strains, isolated from turkey litter (n=74), chicken livers (n=15) and clinical (n=8) samples, were speciated using the pcr-based assay. pcr amplification of the isolates identified a 400-bp cadf gene, conserved in campylobacter species, an 894-bp ceue ge ... | 2005 | 15797819 |
| prevalence and genetic diversity of campylobacter spp. in environmental water samples from a 100-square-kilometer predominantly dairy farming area. | water samples were taken systematically from a 100-km2 area of mainly dairy farmland in northwestern england and examined for campylobacter spp. pulsed-field gel electrophoresis-restriction fragment length polymorphism (pfge-rflp) and flaa strain typing of campylobacter jejuni and campylobacter coli isolates were done. data on the water source and the adjacent environment were recorded and examined as explanatory variables. campylobacter spp. were isolated from 40.5% (n = 119) of the water sampl ... | 2005 | 15812015 |
| differential detection of five mouse-infecting helicobacter species by multiplex pcr. | several species of helicobacter have been isolated from laboratory mice, including h. bilis, h. hepaticus, h. muridarum, h. rodentium, and h. typhlonius, which appear to be the most common. the most widely used published method for molecular detection of these agents is pcr amplification of a conserved region of 16s rrna, but differential speciation requires restriction enzyme digestion of the amplicons. this study was undertaken to determine pcr conditions that would simultaneously and specific ... | 2005 | 15817762 |
| prevalence and diversity of campylobacter jejuni in pig herds on farms with and without cattle or poultry. | the prevalence and diversity of campylobacter jejuni was investigated in pig herds on farms with and without cattle or poultry production. a bacteriological screening of pig cecal samples from 247 finisher herds was carried out at the slaughter-house. subsequently, a follow-up study was conducted in 24 herds (either with or without prior c. jejuni isolation from pigs) in which fecal samples were collected from pigs and, if present, cattle and poultry. samples were analyzed for presence of campyl ... | 2005 | 15830662 |
| [distribution and genetic characterization of porcine campylobacter coli isolates]. | the aim of this study was to evaluate the impact of the slaughter process on the campylobacter (c.) coli prevalence on pig carcasses and finally pork. to detect c. spp., faecal samples, organ samples and surfaces of slaughter pigs were sampled. additionally, various abattoir surfaces (n=208) and 227 pork and minced meat samples were included in our study. whereas a high c. spp. prevalence (64.0%) was detectable in the faeces of slaughter pigs (all isolates were identified as c. coli), low detect ... | 2005 | 15918485 |
| phosphorylation-independent activity of atypical response regulators of helicobacter pylori. | the genome of the gastric pathogen helicobacter pylori harbors a remarkably low number of regulatory genes, including three and five open reading frames encoding two-component histidine kinases and response regulators, respectively, which are putatively involved in transcriptional regulation. two of the response regulator genes, hp1043 and hp166, proved to be essential for cell growth, and inactivation of the response regulator gene hp1021 resulted in a severe growth defect, as indicated by a sm ... | 2005 | 15838037 |
| stable accumulation of sigma54 in helicobacter pylori requires the novel protein hp0958. | several flagellar genes in helicobacter pylori are dependent on sigma(54) (rpon) for their expression. these genes encode components of the basal body, the hook protein, and a minor flagellin, flab. a protein-protein interaction map for h. pylori constructed from a high-throughput screen of a yeast two-hybrid assay (http://pim.hybrigenics.com/pimriderext/common/) revealed interactions between sigma(54) and the conserved hypothetical protein hp0958. to see if hp0958 influences sigma(54) function, ... | 2005 | 15968056 |
| genetic diversity and antibiotic resistance patterns in a campylobacter population isolated from poultry farms in switzerland. | the diversity and genetic interrelation of campylobacter jejuni and c. coli isolated from swiss poultry were assessed by three independent typing methods. samples were derived prior to slaughter from 100 randomly selected flocks (five birds per flock) raised on three different farm types. the observed flock prevalence was 54% in total, with 50% for conventional and 69% for free-range farms. birds held on farms with a confined roaming area had the lowest prevalence of 37%. campylobacter isolates ... | 2005 | 15932975 |
| campylobacter spp., salmonella spp., verocytotoxic escherichia coli, and antibiotic resistance in indicator organisms in wild cervids. | faecal samples were collected, as part of the national health surveillance program for cervids (hop) in norway, from wild red deer, roe deer, moose and reindeer during ordinary hunting seasons from 2001 to 2003. samples from a total of 618 animals were examined for verocytotoxic e. coli (vtec); 611 animals for salmonella and 324 animals for campylobacter. a total of 50 samples were cultivated from each cervid species in order to isolate the indicator bacterial species e. coli and enterococcus fa ... | 2005 | 16108210 |
| [antimicrobial susceptibility of campylobacter jejuni and campylobacter coli isolated from human diarrheic samples]. | one hundred forty-seven campylobacter were isolated using 3,204 samples taken from sporadic diarrheic patients from january 2001 to december 2003. the detection rate of campylobacter in 16 to 30 year old patients (12.9%, 83/641) was significantly higher than that in patients less than 16 years of age, 5%, (29/1,155) (p < 0.001) and more than 30 years of age, 2.5% (35/1,408) (p < 0.001), respectively. the highest detection rate was obtained from the stool of males in the 16 to 30 year old range d ... | 2005 | 15977557 |
| macrolide resistance in campylobacter jejuni and campylobacter coli: molecular mechanism and stability of the resistance phenotype. | a collection of 23 macrolide-resistant campylobacter isolates from different geographic areas was investigated to determine the mechanism and stability of macrolide resistance. the isolates were identified as campylobacter jejuni or campylobacter coli based on the results of the hippurate biochemical test in addition to five pcr-based genotypic methods. three point mutations at two positions within the peptidyl transferase region in domain v of the 23s rrna gene were identified. about 78% of the ... | 2005 | 15980346 |
| agricultural antibiotics and human health. | 2005 | 15984910 | |
| genetic diversity of campylobacter sp. isolates from retail chicken products and humans with gastroenteritis in central michigan. | multilocus sequencing was used to compare campylobacter sp. strains isolated from retail chicken products and humans with gastroenteritis in central michigan. sequence comparisons demonstrated overlapping diversity between chicken and human isolates. campylobacter jejuni isolates from clinical sources had a greater diversity of flagellin alleles and a higher rate of quinolone resistance than isolates from retail chicken products. | 2005 | 16081984 |
| strain persistence and fluctuation of multiple-antibiotic resistant campylobacter coli colonizing turkeys over successive production cycles. | the dynamics of colonization of turkeys by thermophilic campylobacters that are resistant to multiple antibiotics is poorly understood. in this study, we monitored cecal colonization of turkeys by campylobacter over three successive production cycles at the same farm. campylobacter isolated from the ceca was predominantly c. coli in all three flocks. isolates with two distinct fla types that represented a single clonal group based on pulsed-field gel electrophoresis and that were resistant to mu ... | 2005 | 15992304 |
| further characterization of campylobacter isolated from u.s. dairy cows. | the objective of the present study was to compare polymerase chain reaction (pcr) identification with ribotype results and to use pulsed field electrophoresis (pfge) to correlate genotypic patterns with antibiotic resistance of campylobacter isolated from lactating dairy cows in the united states. thirty isolates were studied. twenty-seven of the isolates were identified by pcr as campylobacter jejuni and three were identified as campylobacter coli. genotypic patterns of 15 isolates were determi ... | 2005 | 15992313 |
| effects of subtherapeutic administration of antimicrobial agents to beef cattle on the prevalence of antimicrobial resistance in campylobacter jejuni and campylobacter hyointestinalis. | the influence of antimicrobial agents on the development of antimicrobial resistance (amr) in campylobacter isolates recovered from 300 beef cattle maintained in an experimental feedlot was monitored over a 315-day period (11 sample times). groups of calves were assigned to one of the following antimicrobial treatments: chlortetracycline and sulfamethazine (cs), chlortetracycline alone (ct), virginiamycin, monensin, tylosin phosphate, and no antimicrobial agent (i.e., control treatment). in tota ... | 2005 | 16000800 |
| news and notes: an atypical campylobacter coli exhibiting unusual morphology. | campylobacter coli cells are characterized by a comma, or spiral shape, and a single polar flagellum. here we report stable spontaneous changes in morphology to an aflagellated straight rod. the ability to colonize the chick cecum was lost. the atypical cells were compared with the original c. coli 67 culture from which they were derived, with pcr, riboprinting, and pfge. | 2005 | 16091850 |
| advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16s rrna fluorescence in situ hybridization (fish) detection of campylobacter jejuni, campylobacter coli and campylobacter lari. | traditionally fluorescence in situ hybridization (fish) has been performed with labeled dna oligonucleotide probes. here we present for the first time a high affinity peptide nucleic acid (pna) oligonucleotide sequence for detecting thermotolerant campylobacter spp. using fish. thermotolerant campylobacter spp, including the species campylobacter coli, campylobacter jejuni and campylobacter lari, are important food and water borne pathogens. the designed pna probe (cje195) bound with higher affi ... | 2005 | 16009278 |
| [establishment of immunomagnetic capture-fluorescent pcr detection method for campylobacter jejuni]. | in order to develop a rapid method which can check campylobacter jejuni in animal and poultry foods nicely, an immunomagnetic capture-fluorescent pcr (imc-fpcr) method was established in this paper. the reported method involves isolation of the target pathogen by immunocapture prior to the fluorescent pcr step, therefore the immunomagnetic-beads for campylobacter were developed, and two groups of primer/probe, which targeted for the species special sequence of flaa gene and hipo gene for campylo ... | 2005 | 16013502 |
| resistance of human periodontal ligament fibroblasts to the cytolethal distending toxin of actinobacillus actinomycetemcomitans. | the cytolethal distending toxin (cdt) of actinobacillus actinomycetemcomitans is a typical member of this gram-negative bacterium holotoxin family that targets a wide spectrum of eukarytotic cells, typically causing cell cycle arrest at either the g(1) or g(2)/m phase of the cell cycle. in view of the possible role of the cdt as a prominent a. actinomycetemcomitans virulence factor in periodontal diseases, we have examined the effects of the toxin on primary cultures of human periodontal ligamen ... | 2005 | 16018764 |
| identification of a d-glycero-d-manno-heptosyltransferase gene from helicobacter pylori. | we have identified a helicobacter pylori d-glycero-d-manno-heptosyltransferase gene, hp0479, which is involved in the biosynthesis of the outer core region of h. pylori lipopolysaccharide (lps). insertional inactivation of hp0479 resulted in formation of a truncated lps molecule lacking an alpha-1,6-glucan-, dd-heptose-containing outer core region and o-chain polysaccharide. detailed structural analysis of purified lps from hp0479 mutants of strains ss1, 26695, o:3, and pj1 by a combination of c ... | 2005 | 16030209 |
| antimicrobial resistance patterns of campylobacter from feedlot cattle*. | this study examined 448 campylobacter strains isolated in 1999 and 2000 from us feedlot cattle for resistance to 12 antimicrobials. | 2005 | 16033459 |
| development of real-time pcr and hybridization methods for detection and identification of thermophilic campylobacter spp. in pig faecal samples. | to develop a real-time (rt) pcr for species differentiation of thermophilic campylobacter and to develop a method for assessing co-colonization of pigs by campylobacter spp. | 2005 | 16033460 |
| real-time pcr screening for 16s rrna mutations associated with resistance to tetracycline in helicobacter pylori. | the effectiveness of recommended first-line therapies for helicobacter pylori infections is decreasing due to the occurrence of resistance to metronidazole and/or clarithromycin. quadruple therapies, which include tetracycline and a bismuth salt, are useful alternative regimens. however, resistance to tetracycline, mainly caused by mutations in the 16s rrna genes (rrna and rrnb) affecting nucleotides 926 to 928, are already emerging and can impair the efficacies of such second-line regimens. her ... | 2005 | 16048919 |
| role of efflux pumps and topoisomerase mutations in fluoroquinolone resistance in campylobacter jejuni and campylobacter coli. | point mutations in the topoisomerase (dna gyrase a) gene are known to be associated with fluoroquinolone resistance in campylobacter. recent studies have shown that an efflux pump encoded by cmeabc is also involved in decreased susceptibilities to fluoroquinolones, as well as other antimicrobials. genome analysis suggests that campylobacter jejuni contains at least nine other putative efflux pumps. using insertional inactivation and site-directed mutagenesis, we investigated the potential contri ... | 2005 | 16048946 |