Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| isolation of campylobacter jejuni and campylobacter coli from the gall bladder samples of sheep and identification by polymerase chain reaction. | in this study, 100 gall bladder samples of sheep slaughtered at an abattoir in elaziğ province were examined for campylobacter jejuni and campylobacter coli by culture and polymerase chain reaction (pcr). preston campylobacter agar supplemented with 7% horse blood and preston selective supplement (oxoid, hampshire, uk) were used for isolation of the agents. campylobacter spp. were isolated in 66 samples, and they were identified as 34% c. jejuni and 32% c. coli. a multiplex pcr based upon the us ... | 2003 | 14629001 |
| antimicrobial resistance in campylobacter jejuni and campylobacter coli strains isolated in 1991 and 2001-2002 from poultry and humans in berlin, germany. | the susceptibilities of 430 campylobacter jejuni strains and 79 c. coli strains to six antimicrobial agents were tested and analyzed. the two sets of strains originated from retail market chicken and turkey samples and from humans, respectively, in berlin, germany. two groups of isolates, one dating from 1991 and the other dating from 2001-2002, were tested. of the campylobacter sp. isolates recovered from humans in 2001-2002, 45.1% were resistant to ciprofloxacin, 37.8% were resistant to tetrac ... | 2003 | 14638490 |
| comparison of campylobacter isolates from poultry and humans: association between in vitro virulence properties, biotypes, and pulsed-field gel electrophoresis clusters. | the in vitro virulence properties of 197 temporally and geographically related campylobacter isolates from chicken broilers and humans were compared. comparisons of the virulence properties associated with genotypes and biotypes were made. all isolates adhered to, and 63% invaded, int-407 cells, whereas 13% were cytotoxic for cho cells. cho cell-cytotoxic extracts were also cytotoxic for int-407 cells, but the sensitivity for vero cells was variable. the proportion of isolates demonstrating a hi ... | 2003 | 14532099 |
| risk factors associated with campylobacter jejuni infections in curaçao, netherlands antilles. | a steady increase in the incidence of guillain-barré syndrome (gbs) with a seasonal preponderance, almost exclusively related to campylobacter jejuni, and a rise in the incidence of laboratory-confirmed campylobacter enteritis have been reported from curaçao, netherlands antilles. we therefore investigated possible risk factors associated with diarrhea due to epidemic c. jejuni. typing by pulsed-field gel electrophoresis identified four epidemic clones which accounted for almost 60% of the infec ... | 2003 | 14662945 |
| survival of helicobacter pylori in a natural freshwater environment. | the mode by which helicobacter pylori, the causative agent of most gastric ulcers, is transmitted remains undetermined. epidemiological evidence suggests these organisms are waterborne; however, h. pylori has rarely been grown from potential water sources. this may be due to the ability of this organism to rapidly enter the viable but nonculturable (vbnc) state. our investigation examines the entrance of h. pylori into this state in laboratory cultures and a natural freshwater environment as wel ... | 2003 | 14660399 |
| avian reservoirs and zoonotic potential of the emerging human pathogen helicobacter canadensis. | a polyphasic identification approach was used to investigate the taxonomic position of campylobacter-like isolates recovered from barnacle geese (branta leucopsis) and canada geese (branta candensis). seven strains were selected from a collection of 21 isolates and analyzed by extensive phenotypic testing; four strains were characterized by 16s rrna gene sequence analysis. the results clearly identified the bird isolates as helicobacter canadensis, recently described as an emerging human pathoge ... | 2003 | 14660407 |
| risk factors for antibiotic resistance in campylobacter spp. isolated from raw poultry meat in switzerland. | the world-wide increase of foodborne infections with antibiotic resistant pathogens is of growing concern and is designated by the world health organization as an emerging public health problem. thermophilic campylobacter have been recognised as a major cause of foodborne bacterial gastrointestinal human infections in switzerland and in many other countries throughout the world. poultry meat is the most common source for foodborne cases caused by campylobacter. because all classes of antibiotics ... | 2003 | 14662013 |
| applicability of a rapid duplex real-time pcr assay for speciation of campylobacter jejuni and campylobacter coli directly from culture plates. | a rapid duplex real-time polymerase chain reaction (pcr) assay for speciation of campylobacter jejuni and campylobacter coli using the abi prism 7700 sequence detection system (applied biosystems) was developed based on two of the genes used in a conventional multiplex pcr. a rapid turnaround time of 3 h was achieved with the use of boiled cell lysates. applicability of the assay was tested with 6015 random campylobacter strains referred to the campylobacter reference unit, with 97.6% being iden ... | 2003 | 14680705 |
| antibiotic susceptibility of campylobacter jejuni and campylobacter coli human isolates from bosnia and herzegovina. | 2003 | 12654755 | |
| antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates from broiler chickens isolated at an irish poultry processing plant. | the antibiotic susceptibility of campylobacter jejuni and campylobacter coli isolates from broiler chickens were determined in order to evaluate the level of antibiotic resistance of campylobacter species in the irish poultry industry. | 2003 | 12680938 |
| prevalence of cytolethal distending toxin production in periodontopathogenic bacteria. | cytolethal distending toxin (cdt) is a newly identified virulence factor produced by several pathogenic bacteria implicated in chronic infection. seventy three strains of periodontopathogenic bacteria were examined for the production of cdt by a hela cell bioassay and for the presence of the cdt gene by pcr with degenerative oligonucleotide primers, which were designed based on various regions of the escherichia coli and campylobacter cdtb genes, which have been successfully used for the identif ... | 2003 | 12682119 |
| investigation of infectious agents associated with arthritis by reverse transcription pcr of bacterial rrna. | in reactive and postinfectious arthritis the joints are generally sterile but the presence of bacterial antigens and nucleic acids has been reported. to investigate whether organisms traffic to affected joints in these conditions, we performed reverse transcription pcr using universal primers to amplify any bacterial 16s rrna sequences present in synovial fluid. bacterial sequences were detected in most cases, even after treatment of the synovial fluid with dnase, implying the presence of bacter ... | 2003 | 12716447 |
| investigation of infectious agents associated with arthritis by reverse transcription pcr of bacterial rrna. | in reactive and postinfectious arthritis the joints are generally sterile but the presence of bacterial antigens and nucleic acids has been reported. to investigate whether organisms traffic to affected joints in these conditions, we performed reverse transcription pcr using universal primers to amplify any bacterial 16s rrna sequences present in synovial fluid. bacterial sequences were detected in most cases, even after treatment of the synovial fluid with dnase, implying the presence of bacter ... | 2003 | 12716447 |
| antimicrobial-resistant campylobacter species from retail raw meats. | the antimicrobial susceptibilities of 378 campylobacter isolates were determined. resistance to tetracycline was the most common (82%), followed by resistance to doxycycline (77%), erythromycin (54%), nalidixic acid (41%), and ciprofloxacin (35%). campylobacter coli displayed significantly higher rates of resistance to ciprofloxacin and erythromycin than campylobacter jejuni, and campylobacter isolates from turkey meat showed a greater resistance than those from chicken meat. | 2003 | 12732579 |
| detection of conserved n-linked glycans and phase-variable lipooligosaccharides and capsules from campylobacter cells by mass spectrometry and high resolution magic angle spinning nmr spectroscopy. | glycomics, the study of microbial polysaccharides and genes responsible for their formation, requires the continuous development of rapid and sensitive methods for the identification of glycan structures. in this study, methods for the direct analysis of sugars from 108 to 1010 cells are outlined using the human gastrointestinal pathogen, campylobacter jejuni. using capillary-electrophoresis coupled with sensitive electrospray mass spectrometry, we demonstrate variability in the lipid a componen ... | 2003 | 12716884 |
| inhibition of helicobacter pylori growth in vitro by bulgarian propolis: preliminary report. | bee glue (propolis) possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. the aim of the study was to evaluate the inhibitory effect of bulgarian propolis on helicobacter pylori growth in vitro. activity of 30% ethanolic extract of propolis (eep) against 38 clinical isolates of h. pylori was evaluated by using the agar-well diffusion method. ethanol was used as a control. in addition, the effect of propolis on the growth of 26 h. pylori and 18 campylobacter st ... | 2003 | 12721318 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| phenotypic and genotypic differentiation of campylobacter spp. isolated from austrian broiler farms: a comparison. | an identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (pcr-rflp) was developed to differentiate isolates of the genera campylobacter, arcobacter and helicobacter. based on the 16s rrna gene of these genera, pcr amplified a 1216-bp fragment. the amplicons were digested with the restriction enzymes rsai and ecorv. additional differentiation was obtained using a pcr-assay based on the hippuricase gene. genotyping was performed on several re ... | 2003 | 12745378 |
| comparison of recovery methods for freeze-injured listeria monocytogenes, salmonella typhimurium, and campylobacter coli in cell suspensions and associated with pork surfaces. | cells injured as a result of freezing, heating, and acidification treatments may not grow during conventional microbiological procedures owing to the presence of selective agents, compounds, or dyes in the media, impairing the cell's ability to repair itself and grow. injured cells can be recovered by combining selective and nonselective media into a single system. with such combinations, the diffusion of the selective compounds or dyes is controlled, allowing for the resuscitation of injured ce ... | 2003 | 12747688 |
| infectious agent and immune response characteristics of chronic enterocolitis in captive rhesus macaques. | chronic enterocolitis is the leading cause of morbidity in colonies of captive rhesus macaques (macaca mulatta). this study's aim was to identify the common enteric pathogens frequently associated with chronic enterocolitis in normal, immunocompetent rhesus monkeys and to elucidate the influence of this clinical syndrome on the host immune system. we analyzed the fecal specimens from 100 rhesus macaques with or without clinical symptoms of chronic diarrhea. retrospective analysis revealed an inc ... | 2003 | 12819098 |
| pcr detection of seven virulence and toxin genes of campylobacter jejuni and campylobacter coli isolates from danish pigs and cattle and cytolethal distending toxin production of the isolates. | to study the prevalence of seven virulence and toxin genes, and cytolethal distending toxin (cdt) production of campylobacter jejuni and c. coli isolates from danish pigs and cattle. | 2003 | 12752808 |
| antimicrobial resistance of campylobacter jejuni subsp. jejuni strains isolated from humans in 1998 to 2001 in montréal, canada. | the rates of resistance of 51 to 72 human strains of campylobacter jejuni subsp. jejuni isolated annually from 1998 to 2001 in montréal, québec, canada, varied from 1 to 12% for erythromycin, 43 to 68% for tetracycline, and 10 to 47% for ciprofloxacin. in the last years of the study, there was a significant increase in the rate of resistance to ciprofloxacin (p = 0.00003) but not in the rate of resistance to erythromycin (p = 0.056) or tetracycline (p = 0.095) compared to the rate obtained in th ... | 2003 | 12760892 |
| campylobacter--a tale of two protein glycosylation systems. | post-translational glycosylation is a universal modification of proteins in eukarya, archaea and bacteria. two recent publications describe the first confirmed report of a bacterial n-linked glycosylation pathway in the human gastrointestinal pathogen campylobacter jejuni. in addition, an o-linked glycosylation pathway has been identified and characterized in c. jejuni and the related species campylobacter coli. both pathways have similarity to the respective n- and o-linked glycosylation proces ... | 2003 | 12781527 |
| use of pcr for direct detection of campylobacter species in bovine feces. | this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ... | 2003 | 12788747 |
| in vivo tracking of campylobacter jejuni by using a novel recombinant expressing green fluorescent protein. | campylobacter jejuni is a leading cause of food-borne disease in developed countries. the goal of this study was to develop a plasmid-based reporter system with green fluorescent protein (gfp) to facilitate the study of c. jejuni in a variety of niches. c. jejuni transformants harboring the pmek91 gfp gene (gfp)-containing vector were readily detectable by both fluorescence microscopy and flow cytometry. given the ease of detecting these organisms, additional experiments were performed in which ... | 2003 | 12732559 |
| species-specific identification of campylobacters by partial 16s rrna gene sequencing. | species-specific identification of campylobacters is problematic, primarily due to the absence of suitable biochemical assays and the existence of atypical strains. 16s rrna gene (16s rdna)-based identification of bacteria offers a possible alternative when phenotypic tests fail. therefore, we evaluated the reliability of 16s rdna sequencing for the species-specific identification of campylobacters. sequence analyses were performed by using almost 94% of the complete 16s rrna genes of 135 phenot ... | 2003 | 12791878 |
| reduction of bacteria on pork carcasses associated with chilling method. | in addition to reducing the temperature of pork carcasses immediately after slaughter and before fabrication, blast chilling (snap chill) or conventional chilling can reduce bacterial populations associated with fresh meats. however, there is little information on bacteria survival resulting from the freeze or chill injury of meat products. in this study, porcine fecal slurries with and without pathogens (listeria monocytogenes, salmonella typhimurium, and campylobacter coli) were inoculated ont ... | 2003 | 12801003 |
| campylobacter coli - an important foodborne pathogen. | campylobacters are the most common bacterial cause of infectious intestinal disease (iid) in temperate countries. c. jejuni is the predominant cause of campylobacter iid, but the impact of other, less prevalent species has largely been ignored. here, we present estimates of the burden of indigenously acquired foodborne disease (ifd) due to campylobacter coli, the second most common cause of human campylobacteriosis. | 2003 | 12850159 |
| helicobacter pylori preferentially induces interleukin 12 (il-12) rather than il-6 or il-10 in human dendritic cells. | dendritic cells are potent antigen-presenting cells that are present in the gastrointestinal tract and are required for the induction of a th1 t-cell acquired immune response. since infection with the gastric pathogen helicobacter pylori elicits a th1 cell response, the interaction of these organisms with dendritic cells should reflect the th1 bias. we incubated h. pylori with cultured human dendritic cells and measured the cytokine induction profile, comparing the response to that induced by sa ... | 2003 | 12819109 |
| comparison of eight phenotypic methods for subspecies characterization of thermophilic campylobacter spp. isolated from pig liver. | four hundred pork livers from bacon pigs (37 herds) obtained at six pig-processing plants were studied to assess the campylobacter contamination rate. deep tissue areas were sampled immediately after evisceration. approximately 6% of livers were infected with campylobacter spp., including campylobacter coli (67%), campylobacter jejuni (30%), and campylobacter lari (3%). the 60 resulting isolates (39 c. coli isolates, 19 c. jejuni isolates, and 2 c. lari isolates) employed in this study were char ... | 2003 | 12801013 |
| [antibody response following campylobacter infections determined by elisa]. | an enzyme-linked immunosorbent assay (elisa) was adapted to measure immunoglobulin g (igg), igm, and iga classes of human serum antibody to campylobacter jejuni and campylobacter coli. a total of 631 sera from 210 patients with verified campylobacter enteritis were examined at various intervals after infection, and a control group of 164 sera were tested to determine the cut-off for negative results. with 90 percentile specificity, igg, igm, and iga showed a sensitivity of 71, 60, and 80%, respe ... | 2003 | 12872469 |
| sources of campylobacter colonization in broiler chickens. | 2003 | 12902214 | |
| effect of incubation temperature on isolation of campylobacter jejuni genotypes from foodstuffs enriched in preston broth. | preston broth and agar incubated at either 37 or 42 degrees c have been widely used to isolate campylobacters from foodstuffs. the consequences of using either incubation temperature were investigated. retail packs of raw chicken (n = 24) and raw lamb liver (n = 30) were purchased. samples were incubated in preston broth at 37 and 42 degrees c and then streaked onto preston agar and incubated as before. two campylobacter isolates per treatment were characterized. poultry isolates were genotyped ... | 2003 | 12902254 |
| rapid detection of campylobacter coli, c. jejuni, and salmonella enterica on poultry carcasses by using pcr-enzyme-linked immunosorbent assay. | contamination of retail poultry by campylobacter spp. and salmonella enterica is a significant source of human diarrheal disease. isolation and identification of these microorganisms require a series of biochemical and serological tests. in this study, campylobacter ceue and salmonella inva genes were used to design probes in pcr-enzyme-linked immunosorbent assay (elisa), as an alternative to conventional bacteriological methodology, for the rapid detection of campylobacter jejuni, campylobacter ... | 2003 | 12788755 |
| description of a "phoenix" phenomenon in the growth of campylobacter jejuni at temperatures close to the minimum for growth. | when campylobacter jejuni cultures that had been grown in broth at 39 degrees c were subcultured into fresh medium at 30 degrees c, there was a transient period of growth followed by a decline in viable-cell numbers before growth resumed once more. we propose that this complex behavior is the net effect of the growth of inoculum cells followed by a loss of viability due to oxidative stress and the subsequent emergence of a spontaneously arising mutant population that takes over the culture. | 2003 | 12902294 |
| molecular typing of campylobacter jejuni isolates involved in a neonatal outbreak indicates nosocomial transmission. | genotypic typing by restriction fragment length polymorphism and pulsed-field gel electrophoresis showed that two neonates in a neonatal ward were infected with the same campylobacter jejuni strain. isolates from the mother and brother of the index patient were identical to each other but distinct from the neonatal type. genotyping results therefore suggested that the neonatal c. jejuni infection was nosocomial in origin. | 2003 | 12904420 |
| prevalence of multiple antibiotic resistance in 443 campylobacter spp. isolated from humans and animals. | in view of recent findings that a multidrug efflux pump cmeabc exists in campylobacter jejuni, 391 c. jejuni and 52 campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype. | 2003 | 12917241 |
| rapid detection of campylobacter jejuni in chicken rinse water by melting-peak analysis of amplicons in real-time polymerase chain reaction. | five dna extraction protocols for the detection of campylobacter spp. by polymerase chain reaction (pcr) were compared. a method involving triton x-100 produced template dna of sufficient quality to allow the detection of campylobacter jejuni at levels of 100 cfu/ml in pure culture. primers were designed on the basis of the cadf gene sequence. with a sybr green i real-time pcr assay, these primers amplified only sequences present in c. jejuni to produce a product with a melting temperature of 81 ... | 2003 | 12929818 |
| comparison of human and porcine strains of campylobacter coli. | 2003 | 12930063 | |
| sequence analysis of a cryptic plasmid pcj419 from campylobacter jejuni and construction of an escherichia coli-campylobacter shuttle vector. | a small cryptic plasmid, pcj419, was identified in a human clinical isolate of campylobacter jejuni, cloned and sequenced. pcj419 is a circular molecule of 4013 bp with a g+c content of 27.1%. the products of four open reading frames (orfs) share significant sequence similarity with putative proteins from known c. jejuni and campylobacter coli plasmids. orf-1 encodes a putative mobilisation protein (mob). orf-2 and orf-3 encode proteins that have high identity to putative repa and repb proteins, ... | 2003 | 12932741 |
| in vitro activities of new fluoroquinolones against campylobacter jejuni and campylobacter coli isolates obtained from humans in 1980 to 1982 and 1997 to 2001. | the antibacterial activities of three newly developed fluoroquinolones (gatifloxacin, levofloxacin, and moxifloxacin) against a total of 307 gastrointestinal human isolates of campylobacter jejuni and campylobacter coli collected during 1980 to 1982 and 1997 to 2001 were examined and compared to those of ciprofloxacin and the unrelated antibacterial agents, clarithromycin, erythromycin, and tetracycline by using the agar plate dilution method. all of the fluoroquinolones exhibited a good activit ... | 2003 | 12936999 |
| toward an international standard for pcr-based detection of food-borne thermotolerant campylobacters: assay development and analytical validation. | as part of a european research project (food-pcr), we developed a standardized and robust pcr detection assay specific for the three most frequently reported food-borne pathogenic campylobacter species, c. jejuni, c. coli, and c. lari. fifteen published and unpublished pcr primers targeting the 16s rrna gene were tested in all possible pairwise combinations, as well as two published primers targeting the 23s rrna gene. a panel of 150 strains including target and nontarget strains was used in an ... | 2003 | 12957958 |
| development of a sensitive dna microarray suitable for rapid detection of campylobacter spp. | campylobacter is the most common cause of human acute bacterial gastroenteritis worldwide, widely distributed and isolated from human clinical samples as well as from many other different sources. to comply with the demands of consumers for food safety, there is a need for development of a rapid, sensitive and specific detection method for campylobacter. in this study, we present the development of a novel sensitive dna-microarray based detection method, evaluated on campylobacter and non-campyl ... | 2003 | 12944122 |
| natural transformation of campylobacter jejuni requires components of a type ii secretion system. | the human pathogen campylobacter jejuni is one of more than 40 naturally competent bacterial species able to import macromolecular dna from the environment and incorporate it into their genomes. however, in c. jejuni little is known about the genes involved in this process. we used random transposon mutagenesis to identify genes that are required for the transformation of this organism. we isolated mutants with insertions in 11 different genes; most of the mutants are affected in the dna uptake ... | 2003 | 12949093 |
| toward an international standard for pcr-based detection of food-borne thermotolerant campylobacters: validation in a multicenter collaborative trial. | as part of a european research project, the performance of a pcr assay to detect food-borne thermotolerant campylobacters (campylobacter jejuni, c. coli, and c. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. dna from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. the overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) w ... | 2003 | 12957959 |
| microarray-based identification of thermophilic campylobacter jejuni, c. coli, c. lari, and c. upsaliensis. | dna microarrays are an excellent potential tool for clinical microbiology, since this technology allows relatively rapid identification and characterization of microbial and viral pathogens. in the present study, an oligonucleotide microarray was developed and used for the analysis of thermophilic campylobacter spp., the primary food-borne pathogen in the united states. we analyzed four campylobacter species: campylobacter jejuni, c. coli, c. lari, and c. upsaliensis. our assay relies on the pcr ... | 2003 | 12958228 |
| production of cytolethal distending toxins by pathogenic escherichia coli strains isolated from human and animal sources: establishment of the existence of a new cdt variant (type iv). | three types of cytolethal distending toxin (cdt), namely, cdt-i, cdt-ii, and cdt-iii, have been described in escherichia coli. using primers designed for the detection of sequences common to the cdtb genes, we analyzed by pcr a set of 21 cdt-producing e. coli strains of intestinal and extraintestinal origins isolated from human and different animal species in several european countries and in the united states. on the basis of the existing differences in the cdtb genes, cdt-i-, cdt-ii-, and cdt- ... | 2003 | 12958258 |
| resistance to quinolones in campylobacter jejuni and campylobacter coli from danish broilers at farm level. | to investigate the prevalence of quinolone resistance among campylobacter jejuni and camp. coli isolates from danish poultry at the farm level, as well as for the whole country. | 2003 | 12492931 |
| association of helicobacter pylori antioxidant activities with host colonization proficiency. | to assess the importance of two separate antioxidant activities in helicobacter pylori, we tested the abilities of strains with mutations in either tpx (encoding thiolperoxidase) or ahpc (encoding alkyl hydroperoxide reductase [ahpc]) to colonize the stomachs of mice. the tpx strain was clearly more sensitive than the parent strain to both oxygen and cumene hydroperoxide. the strain colonized only 5% of the inoculated mice. two different classes of oxygen-sensitive ahpc mutants in the type strai ... | 2003 | 12496216 |
| antimicrobial susceptibilities of campylobacter strains isolated from finnish subjects infected domestically or from those infected abroad. | the in vitro susceptibilities of 678 campylobacter jejuni and campylobacter coli strains isolated from stool samples of the same number of finnish subjects were studied. a total of 523 patients, representing inhabitants from throughout finland, had not traveled abroad within the 2 weeks prior to becoming ill, whereas 155 persons had presumably acquired their infections abroad. the antimicrobial agents studied were erythromycin, ciprofloxacin, levofloxacin, trovafloxacin, and moxifloxacin. the mi ... | 2003 | 12499176 |
| enhanced surveillance of campylobacter infection in the north west of england 1997-1999. | to identify sources and routes of infection for sporadic cases of campylobacter infection in the north west of england. | 2003 | 12504607 |
| survival and resuscitation of ten strains of campylobacter jejuni and campylobacter coli under acid conditions. | the culturability of 10 strains of campylobacter jejuni and campylobacter coli was studied after the bacteria were exposed to acid conditions for various periods of time. campylobacter cells could not survive 2 h under acid conditions (formic acid at ph 4). the 10 campylobacter strains could not be recovered, even when enrichment media were used. viable cells, however, could be detected by a double-staining (5-cyano-2,3-ditolyl tetrazolium chloride [ctc]-4',6'-diamidino-2-phenylindole [dapi]) te ... | 2003 | 12514068 |
| molecular assays for detection of human metapneumovirus. | the recent description of the respiratory pathogen human metapneumovirus (hmpv) has highlighted a deficiency in current diagnostic techniques for viral agents associated with acute lower respiratory tract infections. we describe two novel approaches to the detection of viral rna by use of reverse transcriptase pcr (rt-pcr). the pcr products were identified after capture onto a solid-phase medium by hybridization with a sequence-specific, biotinylated oligonucleotide probe. the assay was applied ... | 2003 | 12517833 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| molecular structure and evolution of the conjugative multiresistance plasmid pre25 of enterococcus faecalis isolated from a raw-fermented sausage. | plasmid pre25 from enterococcus faecalis transfers resistances against kanamycin, neomycin, streptomycin, clindamycin, lincomycin, azithromycin, clarithromycin, erythromycin, roxithromycin, tylosin, chloramphenicol, and nourseothricin sulfate by conjugation in vitro to e. faecalis jh2-2, lactococcus lactis bu2, and listeria innocua l19. its nucleotide sequence of 50237 base pairs represents the largest, fully sequenced conjugative multiresistance plasmid of enterococci (plasmid 46 (2001) 170). t ... | 2003 | 14597005 |
| birds, migration and emerging zoonoses: west nile virus, lyme disease, influenza a and enteropathogens. | wild birds are important to public health because they carry emerging zoonotic pathogens, either as a reservoir host or by dispersing infected arthropod vectors. in addition, bird migration provides a mechanism for the establishment of new endemic foci of disease at great distances from where an infection was acquired. birds are central to the epidemiology of west nile virus (wnv) because they are the main amplifying host of the virus in nature. the initial spread of wnv in the u.s. along the ea ... | 2003 | 15931279 |
| fluoroquinolone-resistant campylobacter in animal reservoirs: dynamics of development, resistance mechanisms and ecological fitness. | thermophilic campylobacter species, including campylobacter jejuni and campylobacter coli, are responsible for foodborne campylobacteriosis in humans and are increasingly resistant to fluoroquinolone (fq) antimicrobials. the therapeutic use of fq antimicrobial agents in food animal production, particularly in poultry, has become a concern for public health, because the practice may promote the emergence of fq-resistant campylobacter that can be transmitted to humans through the food chain. recen ... | 2003 | 15134291 |
| routes for campylobacter contamination of poultry meat: epidemiological study from hatchery to slaughterhouse. | from april 1998 to march 2000, 18 broiler flocks were followed from the hatchery to the slaughterhouse. campylobacter was not found in the hatchery, 1-day-old chicks or in the rearing house before the arrival of the chicks. the infection of broiler flocks increased continuously during the rearing time, with a total of seven positive flocks at the end of rearing. farms with campylobacter-positive broilers were characterized by the circulation of campylobacter in the environment (puddles, dung hil ... | 2003 | 14959785 |
| characterization of campylobacter isolates recovered from clinically healthy pigs and from sporadic cases of campylobacteriosis in humans. | campylobacter spp. were recovered from 660 (77.6%) of 850 swine cecal contents at the abattoir and from 24 (8.6%) of 278 specimens from sporadic cases of human diarrhea during the same period in the same geographical area. campylobacter coli represented 95.7% of campylobacter isolates recovered from pigs and 8.3% of those isolated from humans. genetic profiles were determined by pulsed-field gel electrophoresis (pfge) using kpni enzyme to characterize the isolates in combination with phenotypic ... | 2004 | 14968951 |
| distribution and characterization of campylobacter spp. from russian poultry. | the distribution of campylobacter spp. on 13 poultry farms (broiler chicken, quail, pheasant, peacock, and turkey) from eight regions (vladimir, vologda, voronezh, kaluga, liptsk, moscow, orenburg, and orel) in russia was surveyed. intestinal materials were plated onto campylobacter-selective medium and plates were incubated microaerobically at 42 degrees c for 24 or 48 h. identification was based on colonial morphology, microscopic examination, and biochemical tests; latex agglutination assays ... | 2004 | 14968953 |
| optimization of pcr-based methods for rapid detection of campylobacter jejuni, campylobacter coli and yersinia enterocolitica serovar 0:3 in wastewater samples. | pcr-based methods were evaluated for their adequacy to assess the removal of pathogens from wastewater samples. for the development and optimization of the methods, samples were taken at two different sites from two different constructed wetlands. campylobacter jejuni/coli and yersinia enterocolitica serogroup 0:3 were selected as model pathogens and enterococcus faecalis as a standard microbiological indicator. the chosen pcr protocols were optimized for wastewater dna extracts in order to obta ... | 2004 | 14975667 |
| transcriptional phase variation at the flhb gene of pseudomonas putida dot-t1e is involved in response to environmental changes and suggests the participation of the flagellar export system in solvent tolerance. | frameshift mutations in a poly(g) track at the flhb gene of pseudomonas putida dot-t1e are responsible for the diminished swimming of this strain on semisolid medium, which contrasts with the high swimming ability of p. putida kt2440, which does not exhibit a poly(g) track at the flhb gene. we previously showed that a mutant lacking flhb was more sensitive to solvents than the wild-type strain (segura et al., j. bacteriol., 183:4127-4133, 2001). in this study, we show that swimming ability corre ... | 2004 | 14996824 |
| multiplex pcr assay for differentiation of helicobacter felis, h. bizzozeronii, and h. salomonis. | helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. these large spiral organisms are phylogenetically highly related to each other. their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. we describe here a multiplex pcr test based on the trna intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrim ... | 2004 | 15004062 |
| [the role of certain campylobacter types in the etiology of enterocolitis]. | in recent decades, medical community has increasingly been calling attention to the importance of campylobacter as an disease-causing agent in humans. nowadays, campylobacter jejuni (c. jejuni) is known as the most frequent bacterial cause of diarrhea worldwide. epidemiological differences of the infections caused by campylobacter, present in the developed and the developing countries, are attributed to the differences of the types of virulence. due to the specificity, and the demanding features ... | 2004 | 15022385 |
| emergence of fluoroquinolone resistance in the native campylobacter coli population of pigs exposed to enrofloxacin. | the effect of a single 5 day enrofloxacin treatment on the native campylobacter coli population in conventionally weaned 5-week-old pigs was investigated.materials: twelve pigs were split into two groups of six: one group was treated with a therapeutic dose (15 mg/pig/day) of enrofloxacin and the other remained untreated to act as the control. campylobacter coli were isolated from faecal samples and tested for ciprofloxacin resistance by measuring mic values. mutations in the quinolone resistanc ... | 2004 | 15028665 |
| sequence polymorphism in the glycosylation island and flagellins of pseudomonas aeruginosa. | a genomic island consisting of 14 open reading frames, orfa to orfn was previously identified in pseudomonas aeruginosa strain pak and shown to be essential for glycosylation of flagellin. dna microarray hybridization analysis of a number of p. aeruginosa strains from diverse origins showed that this island is polymorphic. pcr and sequence analysis confirmed that many p. aeruginosa strains carry an abbreviated version of the island (short island) in which orfd, -e and -h are polymorphic and orfi ... | 2004 | 15028697 |
| occurrence of campylobacter jejuni in pets living with human patients infected with c. jejuni. | campylobacter jejuni was recovered from four dogs (11%) and four cats (33%) living with danish human patients infected with c. jejuni. pulsed-field gel electrophoresis (pfge) analysis revealed the occurrence of the same quinolone-resistant strain in a girl and her dog. c. jejuni isolates with closely related (>95% similarity) pfge profiles occurred in humans and pets from different danish counties. | 2004 | 15004120 |
| prevalence and characteristics of shiga toxin-producing escherichia coli, salmonella spp. and campylobacter spp. isolated from slaughtered sheep in switzerland. | caecum samples collected from 653 slaughtered sheep from two swiss abattoirs were examined. the aim of this study was: (i) to determine the prevalence of shiga toxin-producing escherichia coli (stec), salmonella spp. and campylobacter spp.; (ii) to further characterize isolated strains; and (iii) to discuss the results obtained with their relevance to food safety. the percentage of samples testing positive for stec by a polymerase chain reaction was 29.9%. the prevalence of positive salmonella s ... | 2004 | 15033267 |
| development of a multiplex microarray microsystem. | a hybrid multiplex microarray microsystem has been developed that consists of 32 individually addressable array reaction chambers, supporting the use of multichannel pipettes for addition of up to 8 samples simultaneously. discrimination between campylobacter jejuni and campylobacter coli bacteria was observed in dna samples containing campylobacter spp., with the same specificity and sensitivity as when compared to a full-size microarray. the spinloaded multiplex microarray microsystem describe ... | 2004 | 15052357 |
| direct quantification of campylobacter jejuni and campylobacter lanienae in feces of cattle by real-time quantitative pcr. | campylobacter species are fastidious to culture, and the ability to directly quantify biomass in microbiologically complex substrates using real-time quantitative (rtq) pcr may enhance our understanding of their biology and facilitate the development of efficacious mitigation strategies. this study reports the use of nested rtq-pcr to directly quantify campylobacter jejuni and campylobacter lanienae in cattle feces. for c. jejuni, the single-copy mapa gene was selected. for c. lanienae, the thre ... | 2004 | 15066825 |
| bacterial pathogen incidences in sludge from swedish sewage treatment plants. | this study surveyed the presence of bacterial pathogens in eight swedish sewage treatment plants (stps), with four different treatment methods, focusing on detection of zoonotic bacteria in raw and treated sludge. salmonella spp., listeria monocytogenes, campylobacter coli and jejuni, escherichia coli o157 and indicator bacteria were investigated. samplings were performed from july 2000 to june 2002, resulting in 64 raw sludge samples and 69 treated sludge samples. the samples from raw sludge (6 ... | 2004 | 15087179 |
| molecular characterization of class 1 integrons from irish thermophilic campylobacter spp. | in this study a large random collection (n = 378) of irish thermophilic campylobacter isolates were investigated for the presence of integrons, genetic elements associated with the dissemination of antimicrobial resistance. | 2004 | 15128721 |
| longitudinal study of the excretion patterns of thermophilic campylobacter spp. in young pet dogs in denmark. | the campylobacter excretion patterns of 26 domestic pet dogs were described in a longitudinal study. the dogs entered the study between 3 and 8 months of age and were monitored until 2 years of age. they were tested monthly for campylobacter carriage in stool samples that were cultured on the campylobacter-selective media cat and modified ccda agar at 37 and 42 degrees c. this study comprised 366 fecal swab samples, of which 278 (76.2%) were found to be campylobacter positive, with the following ... | 2004 | 15131162 |
| use of the omp50 gene for identification of campylobacter species by pcr. | we studied the prevalence of the omp50 gene and the omp50 protein in campylobacter strains. immunodetection assays and dna-dna hybridizations showed that most c. coli strains tested were negative and most c. jejuni and c. lari strains tested were positive. a pcr assay was developed, using the omp50 gene as a species-specific target. we propose a combination of a hippurate test and an omp50 assay to perform identification of campylobacter species. | 2004 | 15131219 |
| characterization of a haemolytic phospholipase a(2) activity in clinical isolates of campylobacter concisus. | a membrane-bound, haemolytic phospholipase a(2) (pla(2)) activity was detected in clinical strains of campylobacter concisus isolated from children with gastroenteritis. the clinical strains were assigned into two molecular groups (genomospecies) based on pcr amplification of their 23s rdna. this calcium-dependent, heat-stable, haemolytic pla(2) activity was detected in strains from both genomospecies. a crude haemolysin extract (che) was initially prepared from cellular outer-membrane proteins ... | 2004 | 15150326 |
| cloning and characterization of the gene encoding the major cell-associated phospholipase a of legionella pneumophila, plab, exhibiting hemolytic activity. | legionella pneumophila, the causative agent of legionnaires' disease, is an intracellular pathogen of amoebae, macrophages, and epithelial cells. the pathology of legionella infections involves alveolar cell destruction, and several proteins of l. pneumophila are known to contribute to this ability. by screening a genomic library of l. pneumophila, we found an additional l. pneumophila gene, plab, which coded for a hemolytic activity and contained a lipase consensus motif in its deduced protein ... | 2004 | 15102773 |
| prevalence and antimicrobial resistance of campylobacter coli isolated from fattening pigs in france. | campylobacter are a leading cause of human diarrhea. the usual source of infection is contaminated food, particularly poultry but pork has also been described. the veterinary use of antimicrobial drugs has been suggested to be largely responsible for resistance in human isolates of this zoonotic pathogen. a study was carried out to investigate the occurrence and antimicrobial resistance of campylobacter isolated from french fattening pigs. from march 1998 to june 1999, stomach samples were colle ... | 2004 | 15172691 |
| in vitro inhibitory activity of chinese leek extract against campylobacter species. | three aqueous extracts of dietary materials of chinese leek (leek flower stem, soft leek and green leek) and two other reference extracts of allium plants were used to investigate the antibacterial effects against campylobacter jejuni and campylobacter coli. all the tested strains of campylobacter species were isolated from chickens. the minimum concentration of aqueous leek extracts (ales) required to inhibit the bacterial growth was 2.0 mg/ml. among the plant extracts tested, ales shared the l ... | 2004 | 15193803 |
| relative contribution of target gene mutation and efflux to fluoroquinolone and erythromycin resistance, in french poultry and pig isolates of campylobacter coli. | thirty-eight avian and swine french isolates of campylobacter coli were studied for their mechanisms of co-resistance to fluoroquinolones and erythromycin. a thr86ile modification of gyra, responsible for fluoroquinolone resistance, was found in all the strains. two different levels of resistance to erythromycin (mic of 8-16 or >/=256 mg/l) were observed. a a2075g mutation in the 23s rrna genes was found only in the highly-resistant strains. phe-arg-beta-naphthylamide, an efflux pump inhibitor, ... | 2004 | 15120725 |
| dual control of helicobacter pylori heat shock gene transcription by hspr and hrca. | the hspr repressor regulates transcription of the groesl, hrca-grpe-dnak, and cbpa-hspr-orf operons of helicobacter pylori. here we show that two of the hspr-regulated operons, namely, the groesl and dnak operons, encoding the major cellular chaperone machineries are also regulated by the h. pylori homologue of the hrca repressor. similarly to the hspr mutation, deletion of the hrca gene also leads to complete derepression of the pgro and phrc promoters. the presence of both hspr and hrca is the ... | 2004 | 15126455 |
| antigenicity of the campylobacter coli cjaa protein produced by escherichia coli. | immunogenic c. coli cjaa protein is a candidate for a chicken anti-campylobacter subunit vaccine. in order to enhance its immunogenicity an antigenic determinant of the cjaa protein was identified. thereafter, two copies of the antigenic epitope were cloned in tandem directly or with a flexible hinge between them. all experiments documented that the cjaa protein contained not only linear antigenic epitope/s but also conformational ones. | 2004 | 15330269 |
| frequency and spatial distribution of environmental campylobacter spp. | humans are exposed to campylobacter spp. in a range of sources via both food and environmental pathways. for this study, we explored the frequency and distribution of thermophilic campylobacter spp. in a 10- by 10-km square rural area of cheshire, united kingdom. the area contains approximately 70, mainly dairy, farms and is used extensively for outdoor recreational activities. campylobacter spp. were isolated from a range of environmental samples by use of a systematic sampling grid. livestock ... | 2004 | 15528512 |
| detection of campylobacter spp. in chicken fecal samples by real-time pcr. | a real-time pcr assay for detecting thermophilic campylobacter spp. directly in chicken feces has been developed. dna was isolated from fecal material by using magnetic beads followed by pcr with a prealiquoted pcr mixture, which had been stored at -18 degrees c. campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 cfu/ml, in a fecal suspension. a bacterial internal control was added before dna extraction to control both dna isolation and the presence of pcr inh ... | 2004 | 15528705 |
| sensitive and specific detection of yersinia pseudotuberculosis by loop-mediated isothermal amplification. | we developed a loop-mediated isothermal amplification method able to detect yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. this method is more sensitive than pcr and might be a useful tool for detecting and identifying y. pseudotuberculosis. | 2004 | 15528740 |
| insufficient production and tissue delivery of cd4+ memory t cells in rapidly progressive simian immunodeficiency virus infection. | the mechanisms linking human immunodeficiency virus replication to the progressive immunodeficiency of acquired immune deficiency syndrome are controversial, particularly the relative contribution of cd4+ t cell destruction. here, we used the simian immunodeficiency virus (siv) model to investigate the relationship between systemic cd4+ t cell dynamics and rapid disease progression. of 18 rhesus macaques (rms) infected with ccr5-tropic sivmac239 (n=14) or cxcr4-tropic sivmac155t3 (n=4), 4 of the ... | 2004 | 15545355 |
| campylobacter insulaenigrae sp. nov., isolated from marine mammals. | phenotypic and phylogenetic studies were performed on four campylobacter-like organisms recovered from three seals and a porpoise. comparative 16s rrna gene sequencing studies demonstrated that the organisms represent a hitherto unknown subline within the genus campylobacter, associated with a subcluster containing campylobacter jejuni, campylobacter coli and campylobacter lari. dna-dna hybridization studies confirmed that the bacteria belonged to a single species, for which the name campylobact ... | 2004 | 15545485 |
| characterization of lactobacillus coryniformis dsm 20001t surface protein cpf mediating coaggregation with and aggregation among pathogens. | phenotypic characterization of aggregation phenotypes of lactobacillus coryniformis revealed that strain dsm 20001t coaggregated with escherichia coli k88, campylobacter coli, and campylobacter jejuni but not with other human pathogens. in addition, cells of these pathogens aggregated in the presence of the spent culture supernatant (scs) of strain dsm 20001t. cells of e. coli k88 remained viable in the coaggregates and aggregates for up to 24 h. both coaggregation and aggregation (co/aggregatio ... | 2004 | 15574903 |
| regional risks and seasonality in travel-associated campylobacteriosis. | the epidemiology of travel-associated campylobacteriosis is still largely unclear, and various known risk factors could only explain limited proportions of the recorded cases. | 2004 | 15569393 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| survival of cold-stressed campylobacter jejuni on ground chicken and chicken skin during frozen storage. | campylobacter jejuni is prevalent in poultry, but the effect of combined refrigerated and frozen storage on its survival, conditions relevant to poultry processing and storage, has not been evaluated. therefore, the effects of refrigeration at 4 degrees c, freezing at -20 degrees c, and a combination of refrigeration and freezing on the survival of c. jejuni in ground chicken and on chicken skin were examined. samples were enumerated using tryptic soy agar containing sheep's blood and modified c ... | 2004 | 15574906 |
| use of midi-fatty acid methyl ester analysis to monitor the transmission of campylobacter during commercial poultry processing. | the presence of campylobacter spp. on broiler carcasses and in scald water taken from a commercial poultry processing facility was monitored on a monthly basis from january through june. campylobacter agar, blaser, was used to enumerate campylobacter in water samples from a multiple-tank scalder; on prescalded, picked, eviscerated, and chilled carcasses; and on processed carcasses stored at 4 degrees c for 7 or 14 days. the midi sherlock microbial identification system was used to identify campy ... | 2004 | 15330523 |
| global transposon mutagenesis and essential gene analysis of helicobacter pylori. | we have constructed a genome-saturating mutant library of the human gastric pathogen helicobacter pylori. microarray tracking of transposon mutants (matt) allowed us to map the position of 5,363 transposon mutants in our library. while we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes. comparison to the essential gene set of other bacteria revealed a surpris ... | 2004 | 15547264 |
| antimicrobial resistance in campylobacter strains isolated from french broilers before and after antimicrobial growth promoter bans. | the antimicrobial susceptibility of campylobacter strains isolated from standard and free-range broilers in 1992-1996 and 2001-2002 was studied. | 2004 | 15537699 |
| in vitro and in vivo characterization of helicobacter hepaticus cytolethal distending toxin mutants. | helicobacter hepaticus expresses a member of the cytolethal distending toxin (cdt) family of bacterial cytotoxins. to investigate the role of cdt in the pathogenesis of h. hepaticus, transposon mutagenesis was used to generate a series of isogenic mutants in and around the cdtabc gene cluster. an h. hepaticus transposon mutant with a disrupted cdtabc coding region no longer produced cdt activity. conversely, a transposon insertion outside of the cluster did not affect the cdt activity. an examin ... | 2004 | 15102759 |
| antimicrobial resistance among enteric pathogens. | diarrhea is a major cause of morbidity and mortality in infants and children worldwide. use of antimicrobial therapy in children with bacterial diarrhea involves consideration of advantages and limitations of use of appropriate agents in the general population and in specific hosts. antimicrobial agents for bacterial diarrhea should be prescribed with an appreciation of limitations including antimicrobial resistance. studies from many countries have reported resistance to campylobacter jejuni, c ... | 2004 | 15185189 |
| characterization of erythromycin resistance in campylobacter coli and campylobacter jejuni isolated from pig offal in new zealand. | to determine the level and mechanism(s) of antimicrobial resistance in campylobacter isolates obtained from human and environmental sources from south canterbury, new zealand. | 2004 | 15186451 |
| genotyping of broiler-originated campylobacter jejuni and campylobacter coli isolates using fla typing and random amplified polymorphic dna methods. | liver and intestine samples taken from 200 broilers at 20 flocks were inoculated onto preston enrichment broth and agar for selective isolation of campylobacter jejuni and campylobacter coli. the isolates were identified by both conventional and polymerase chain reaction (pcr) methods. campylobacter spp. were identified in 102 of 400 samples (200 liver and 200 intestine), 57 (14.25%) of which were identified as c. jejuni and 45 (11.25%) as c. coli. pcr-restriction fragment length polymorphism (r ... | 2004 | 15193806 |
| relapsing cellulitis associated with campylobacter coli bacteremia in an agammaglobulinemic patient. | campylobacter coli rarely causes bacteremia or extraintestinal infection. we report herein a case of agammaglobulinemia in which cellulitis associated with c. coli bacteremia relapsed after a disease-free interval of >5 years. pulsed field gel electrophoresis revealed that the organisms in this patient were genetically identical, suggesting a latent c. coli infection. | 2004 | 15194845 |
| quinolone-resistant campylobacter infections: risk factors and clinical consequences. | we integrated data on quinolone and macrolide susceptibility patterns with epidemiologic and typing data from campylobacter jejuni and c. coli infections in two danish counties. the mean duration of illness was longer for 86 patients with quinolone-resistant c. jejuni infections (median 13.2 days) than for 381 patients with quinolone-sensitive c. jejuni infections (median 10.3 days, p = 0.001). foreign travel, eating fresh poultry other than chicken and turkey, and swimming were associated with ... | 2004 | 15207057 |
| adaptation of campylobacter jejuni nctc11168 to high-level colonization of the avian gastrointestinal tract. | the genome sequence of the human pathogen campylobacter jejuni nctc11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that this strain is a poor colonizer. experimental colonization and persistence studies were carried out with c. jejuni nctc11168 by using 2-week-old light sussex chickens possessing an acquired natural gut flora. after inoculation, nctc11168 initially colonized the intestine poorly. however, after 5 weeks ... | 2004 | 15213117 |