Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
solexaqa: at-a-glance quality assessment of illumina second-generation sequencing data.illumina's second-generation sequencing platform is playing an increasingly prominent role in modern dna and rna sequencing efforts. however, rapid, simple, standardized and independent measures of run quality are currently lacking, as are tools to process sequences for use in downstream applications based on read-level quality data.201020875133
microbial diagnostic microarray for food- and water-borne pathogens.a microbial diagnostic microarray for the detection of the most relevant bacterial food- and water-borne pathogens and indicator organisms was developed and thoroughly validated. the microarray platform based on sequence-specific end labelling of oligonucleotides and the pyhylogenetically robust gyrb marker gene allowed a highly specific (resolution on genus/species level) and sensitive (0.1% relative and 10(4) cfu absolute detection sensitivity) detection of the target pathogens. validation was ...201021255342
methods for determining disease burden and calibrating national surveillance data in the united kingdom: the second study of infectious intestinal disease in the community (iid2 study).infectious intestinal disease (iid), usually presenting as diarrhoea and vomiting, is frequently preventable. though often mild and self-limiting, its commonness makes iid an important public health problem. in the mid 1990s around 1 in 5 people in england suffered from iid a year, costing around pound0.75 billion. no routine information source describes the uk's current community burden of iid. we present here the methods for a study to determine rates and aetiology of iid in the community, pre ...201020444246
prevalence of virulence-associated genes and cytolethal distending toxin production in campylobacter spp. isolated in italy.the prevalence of virulence and cytolethal distending toxin (cdt) genes and the cytotoxic activity in vero and hep-2 cells was estimated in 29 campylobacter jejuni and 36 campylobacter coli from foods, animals and humans isolates. all c. jejuni showed flaa, cadf, cdta, cdtb, cdtc and cdt cluster genes fragments, except for ceue (86.2%) and cdt genes (93.1%). amongst c. coli strains, a lower prevalence of ceue gene (83.3%) was detected than that for cdta, cdtb, cdtc genes (97.2%), cdt gene cluste ...201019195703
inaccuracy of routine susceptibility tests for detection of erythromycin resistance of campylobacter jejuni and campylobacter coli.in the netherlands, both an increase in and regional differences in erythromycin resistance of campylobacter jejuni and campylobacter coli have been reported. to determine the accuracy of routine tests for erythromycin resistance, 48 erythromycin-resistant isolates from various laboratories that participate in the dutch surveillance of campylobacter infections were reinvestigated. initial susceptibility testing for erythromycin had been performed by disk diffusion in six and mic-based methods in ...201019438640
demonstration of persistent strains of campylobacter jejuni within broiler farms over a 1-year period in lithuania.the aim of the study was to investigate the flock prevalence of campylobacter jejuni and campylobacter coli in broiler farms in lithuania and to identify possible persistent strains of camp. jejuni using amplified fragment length polymorphism (aflp) typing method.201019709341
dna identification and characterization of campylobacter jejuni and campylobacter coli isolated from caecal samples of chickens in grenada.to speciate campylobacter strains from the caeca of chickens in grenada using pcr and to evaluate dna-based typing methods for the characterization of these isolates.201019735321
investigation of food and environmental exposures relating to the epidemiology of campylobacter coli in humans in northwest england.this study uses multilocus sequence typing (mlst) to investigate the epidemiology of campylobacter coli in a continuous study of a population in northwest england. all cases of campylobacter identified in four local authorities (government administrative boundaries) between 2003 and 2006 were identified to species level and then typed, using mlst. epidemiological information was collected for each of these cases, including food and recreational exposure variables, and the epidemiologies of c. je ...201019854914
a genomic island defines subspecies-specific virulence features of the host-adapted pathogen campylobacter fetus subsp. venerealis.the pathogen campylobacter fetus comprises two subspecies, c. fetus subsp. fetus and c. fetus subsp. venerealis. although these taxa are highly related on the genome level, they are adapted to distinct hosts and tissues. c. fetus subsp. fetus infects a diversity of hosts, including humans, and colonizes the gastrointestinal tract. in contrast, c. fetus subsp. venerealis is largely restricted to the bovine genital tract, causing epidemic abortion in these animals. in light of their close genetic ...201019897645
a genomic island defines subspecies-specific virulence features of the host-adapted pathogen campylobacter fetus subsp. venerealis.the pathogen campylobacter fetus comprises two subspecies, c. fetus subsp. fetus and c. fetus subsp. venerealis. although these taxa are highly related on the genome level, they are adapted to distinct hosts and tissues. c. fetus subsp. fetus infects a diversity of hosts, including humans, and colonizes the gastrointestinal tract. in contrast, c. fetus subsp. venerealis is largely restricted to the bovine genital tract, causing epidemic abortion in these animals. in light of their close genetic ...201019897645
comparison between the biofilm initiation of campylobacter jejuni and campylobacter coli strains to an inert surface using biofilm ring test.the adhesion to an inert surface (the first step of biofilm formation) of the two main pathogenic campylobacter species, campylobacter jejuni and campylobacter coli, isolated from diverse origins, was compared.201019796124
diversity of campylobacter jejuni and campylobacter coli genotypes from human and animal sources from rio de janeiro, brazil.to compare the genotypes of campylobacter jejuni and campylobacter coli isolates of human and animal origin collected in rio de janeiro city, 30 c. jejuni and 35 c. coli isolates from animal sources (n=45) and human patients with gastroenteritis (n=20) were genotyped by pcr-based techniques, namely random amplified polymorphic dna (rapd-pcr) and enterobacterial repetitive intergenic consensus sequence (eric-pcr). rapd-pcr identified 50 types and eric-pcr identified 22 genotypes, among the 65 cam ...201019765787
prolonged survival of campylobacter species in bovine manure compost.the persistence of naturally occurring campylobacteria in aerobic compost constructed of manure from beef cattle that were administered chlortetracycline and sulfamethazine (as700) or from cattle not administered antibiotics (control) was examined. although there were no differences in population sizes of heterotrophic bacteria, the temperature of as700 compost was more variable and did not become as high as that of control compost. there were significant differences in water content, total carb ...201020023098
comparison of pcr binary typing (p-bit), a new approach to epidemiological subtyping of campylobacter jejuni, with serotyping, pulsed-field gel electrophoresis, and multilocus sequence typing methods.to overcome some of the deficiencies with current molecular typing schema for campylobacter spp., we developed a prototype pcr binary typing (p-bit) approach. we investigated the distribution of 68 gene targets in 58 campylobacter jejuni strains, one campylobacter lari strain, and two campylobacter coli strains for this purpose. gene targets were selected on the basis of distribution in multiple genomes or plasmids, and known or putative status as an epidemicity factor. strains were examined wit ...201020023103
activation of human and chicken toll-like receptors by campylobacter spp.campylobacter infection in humans is accompanied by severe inflammation of the intestinal mucosa, in contrast to colonization of chicken. the basis for the differential host response is unknown. toll-like receptors (tlrs) sense and respond to microbes in the body and participate in the induction of an inflammatory response. thus far, the interaction of campylobacter with chicken tlrs has not been studied. here, we investigated the potential of four campylobacter strains to activate human tlr1/2/ ...201020038539
activation of human and chicken toll-like receptors by campylobacter spp.campylobacter infection in humans is accompanied by severe inflammation of the intestinal mucosa, in contrast to colonization of chicken. the basis for the differential host response is unknown. toll-like receptors (tlrs) sense and respond to microbes in the body and participate in the induction of an inflammatory response. thus far, the interaction of campylobacter with chicken tlrs has not been studied. here, we investigated the potential of four campylobacter strains to activate human tlr1/2/ ...201020038539
antimicrobial susceptibilities of multidrug-resistant campylobacter jejuni and c. coli strains: in vitro activities of 20 antimicrobial agents.there is a paucity of information regarding antimicrobial agents that are suitable to treat severe infections caused by multidrug-resistant campylobacter spp. our aim was to identify agents that are potentially effective against multiresistant campylobacter strains. the in vitro activities of 20 antimicrobial agents against 238 campylobacter strains were analyzed by determining mics by the agar plate dilution method or the etest. these strains were selected from 1,808 campylobacter isolates coll ...201020038624
species-specific identification and differentiation of arcobacter, helicobacter and campylobacter by full-spectral matrix-associated laser desorption/ionization time of flight mass spectrometry analysis.rapid and reliable identification of arcobacter and helicobacter species, and their distinction from phenotypically similar campylobacter species, has become increasingly important, since many of them are now recognized as human and/or animal pathogens. matrix-associated laser desorption/ionization-time of flight (maldi-tof) ms has been shown to be a rapid and sensitive method for characterization of micro-organisms. in this study, we therefore established a reference database of selected arcoba ...201019959629
study of cytolethal distending toxin (cdt) in campylobacter coli using a multiplex polymerase chain reaction assay and its distribution among clinical and food strains.from a collection of 242 food isolates of campylobacter spp. 72 were identified as campylobacter jejuni and 170 as campylobacter coli and of 112 isolates from clinical sources, 92 were identified as c. jejuni and 20 as c. coli. the results for food isolates were in contrast to other reported identifications. twenty c. coli strains from clinical cases of campylobacter that occurred in portugal and 170 isolated from poultry products were examined for the presence of cytolethal distending toxin (cd ...201019821742
development and evaluation of internal amplification controls for use in a real-time duplex pcr assay for detection of campylobacter coli and campylobacter jejuni.a common problem of both conventional and real-time pcr assays is failure of dna amplification due to the presence of inhibitory substances in samples. in view of this, our aim was to develop and evaluate internal amplification controls (iacs) for use with an existing duplex real-time pcr assay for campylobacter coli and campylobacter jejuni. both competitive and non-competitive iacs were developed and evaluated. the competitive approach involved a dna fragment of the coding region of the fish v ...201019833779
development of a dna microarray to detect antimicrobial resistance genes identified in the national center for biotechnology information database.to understand the mechanisms and epidemiology of antimicrobial resistance (ar), the genetic elements responsible must be identified. due to the myriad of possible genes, a high-density genotyping technique is needed for initial screening. to achieve this, ar genes in the national center for biotechnology information genbank database were identified by their annotations and compiled into a nonredundant list of 775 genes. a dna microarray was constructed of 70mer oligonucelotide probes designed to ...201019916789
recrudescent campylobacter jejuni infection in an immunocompetent adult following experimental infection with a well-characterized organism.the recrudescence of infection with campylobacter jejuni after appropriate antibiotic treatment has not been previously reported in an immunocompetent adult. we present the complete clinical, microbiologic, and immunologic evaluation of a closely monitored healthy male with recrudescent c. jejuni infection occurring in the absence of immunodeficiency following experimental infection with a well-characterized strain. after antibiotic treatment, the initial infection was clinically cleared and mic ...201019923572
recrudescent campylobacter jejuni infection in an immunocompetent adult following experimental infection with a well-characterized organism.the recrudescence of infection with campylobacter jejuni after appropriate antibiotic treatment has not been previously reported in an immunocompetent adult. we present the complete clinical, microbiologic, and immunologic evaluation of a closely monitored healthy male with recrudescent c. jejuni infection occurring in the absence of immunodeficiency following experimental infection with a well-characterized strain. after antibiotic treatment, the initial infection was clinically cleared and mic ...201019923572
multi-locus sequence types of campylobacter carried by flies and slugs acquired from local ruminant faeces.to assess whether flies and slugs acquire strains of campylobacter jejuni and campylobacter coli present in local ruminant faeces.201020337762
modification of the campylobacter jejuni flagellin glycan by the product of the cj1295 homopolymeric-tract-containing gene.the campylobacter jejuni flagellin protein is o-glycosylated with structural analogues of the nine-carbon sugar pseudaminic acid. the most common modifications in the c. jejuni 81-176 strain are the 5,7-di-n-acetylated derivative (pse5ac7ac) and an acetamidino-substituted version (pse5am7ac). other structures detected include o-acetylated and n-acetylglutamine-substituted derivatives (pse5am7ac8oac and pse5am7ac8glnnac, respectively). recently, a derivative of pseudaminic acid modified with a di ...201020338909
growth phase-dependent activation of the dccrs regulon of campylobacter jejuni.two-component systems are widespread prokaryotic signal transduction devices which allow the regulation of cellular functions in response to changing environmental conditions. the two-component system dccrs (cj1223c-cj1222c) of campylobacter jejuni is important for the colonization of chickens. here, we dissect the dccrs system in more detail and provide evidence that the sensor dccs selectively phosphorylates the cognate effector, dccr. microarray expression profiling, real-time reverse transcr ...201020348251
the internal transcribed spacer region, a new tool for use in species differentiation and delineation of systematic relationships within the campylobacter genus.the campylobacter genus consists of a number of important human and animal pathogens. although the 16s rrna gene has been used extensively for detection and identification of campylobacter species, there is currently limited information on the 23s rrna gene and the internal transcribed spacer (its) region that lies between the 16s and 23s rrna genes. we examined the potential of the 23s rrna gene and the its region to be used in species differentiation and delineation of systematic relationships ...201020348308
evidence for a lineage of virulent bacteriophages that target campylobacter.our understanding of the dynamics of genome stability versus gene flux within bacteriophage lineages is limited. recently, there has been a renewed interest in the use of bacteriophages as 'therapeutic' agents; a prerequisite for their use in such therapies is a thorough understanding of their genetic complement, genome stability and their ecology to avoid the dissemination or mobilisation of phage or bacterial virulence and toxin genes. campylobacter, a food-borne pathogen, is one of the organi ...201020353581
antimicrobial resistance, virulence, and genotypic profile comparison of campylobacter jejuni and campylobacter coli isolated from humans and retail meats.a total of 360 spatially and temporally related campylobacter isolates, including 168 from clinical human cases (campylobacter jejuni n = 148; campylobacter coli n = 20) and 192 from retail meats (c. jejuni n = 114; c. coli n = 78), were analyzed for antimicrobial susceptibilities, virulence, and genotypic profiles. ciprofloxacin-resistant c. jejuni was observed in 13.5% and 19% of the isolates from humans and retail chicken breasts, respectively. antimicrobial resistance to ciprofloxacin and er ...201020367499
evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts.the aim of this study was to evaluate diffusion and dilution methods for determining the antibacterial activity of plant extracts and their mixtures. several methods for measurement of the minimal inhibitory concentration (mic) of a plant extract are available, but there is no standard procedure as there is for antibiotics. we tested different plant extracts, their mixtures and phenolic acids on selected gram-positive (staphylococcus aureus, bacillus cereus, and listeria monocytogenes) and gram- ...201020171250
effects of polyphosphate additives on campylobacter survival in processed chicken exudates.campylobacter spp. are responsible for a large number of the bacterial food poisoning cases worldwide. despite being sensitive to oxygen and nutritionally fastidious, campylobacter spp. are able to survive in food processing environments and reach consumers in sufficient numbers to cause disease. to investigate campylobacter persistence on processed chicken, exudates from chickens produced for consumer sale were collected and sterilized. two types of exudates from chicken products were collected ...201020173061
phylogenomics reveals a diverse rickettsiales type iv secretion system.with an obligate intracellular lifestyle, alphaproteobacteria of the order rickettsiales have inextricably coevolved with their various eukaryotic hosts, resulting in small, reductive genomes and strict dependency on host resources. unsurprisingly, large portions of rickettsiales genomes encode proteins involved in transport and secretion. one particular transporter that has garnered recent attention from researchers is the type iv secretion system (t4ss). homologous to the well-studied archetyp ...201020176788
prevalence and antimicrobial resistance of campylobacter species isolated from raw camel, beef, lamb, and goat meat in iran.campylobacter spp. are one of the most common causes of acute bacterial gastroenteritis in human beings which are transmitted mostly via food originating from animals. this study was conducted to determine the prevalence and antimicrobial resistance of campylobacter spp. isolated from retail raw meats in iran. from june 2008 to june 2009, a total of 722 raw meat samples from camel (n = 107), beef (n = 190), lamb (n = 225), and goat (n = 180) were purchased from randomly selected retail outlets i ...201020184450
virulence and immunomodulatory roles of bacterial outer membrane vesicles.outer membrane (om) vesicles are ubiquitously produced by gram-negative bacteria during all stages of bacterial growth. om vesicles are naturally secreted by both pathogenic and nonpathogenic bacteria. strong experimental evidence exists to categorize om vesicle production as a type of gram-negative bacterial virulence factor. a growing body of data demonstrates an association of active virulence factors and toxins with vesicles, suggesting that they play a role in pathogenesis. one of the most ...201020197500
reduction of campylobacter jejuni and campylobacter coli in poultry skin by fruit extracts.campylobacter spp. are a major cause of foodborne bacterial gastroenteritis in humans, and current methods to control campylobacter contamination in foods are not completely successful. plants are a promising source of antimicrobial agents, particularly given the growing interest in "all natural" foods. in this study, the antimicrobial activity of extracts from 28 edible plants against campylobacter jejuni and campylobacter coli was evaluated in vitro and in a poultry skin model. nine of 28 extr ...201020202332
mass spectrometric analysis of ehrlichia chaffeensis tandem repeat proteins reveals evidence of phosphorylation and absence of glycosylation.ehrlichia chaffeensis has a small subset of immunoreactive secreted, acidic (pi approximately 4), tandem repeat (tr)-containing proteins (trps), which exhibit abnormally large electrophoretic masses that have been associated with glycosylation of the tr domain.201020209062
performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory.matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has recently been introduced in diagnostic microbiology laboratories for the identification of bacterial and yeast strains isolated from clinical samples. in the present study, we prospectively compared maldi-tof ms to the conventional phenotypic method for the identification of routine isolates. colonies were analyzed by maldi-tof ms either by direct deposition on the target plate or after a formic acid- ...201020220166
risk factors for indigenous campylobacter jejuni and campylobacter coli infections in the netherlands: a case-control study.a case-control study comprising 1315 campylobacter jejuni cases, 121 campylobacter coli cases and 3409 frequency-matched controls was conducted in the netherlands in 2002-2003. risk factors for both c. jejuni and c. coli enteritis were consumption of undercooked meat and barbecued meat, ownership of cats and use of proton pump inhibitors. consumption of chicken was a predominant risk factor for c. jejuni enteritis, but many additional risk factors were identified. unique risk factors for c. coli ...201020223048
phenotypic and genotypic antimicrobial resistance profiles of campylobacter jejuni isolated from cattle, sheep, and free-range poultry faeces.minimum inhibitory concentrations (mic) of 13 antimicrobial agents were determined by broth microdilution for 72 campylobacter jejuni strains from livestock. twenty-three (31.9%) isolates were fully susceptible; all isolates were susceptible to erythromycin, chloramphenicol, streptomycin, gentamicin, sulfamethoxazole, and meropenem, and all but one to kanamycin. resistance to quinolones was highest (52.8%), reaching similar values among poultry, dairy cattle, and sheep, but lower in beef cattle. ...201020224816
phenotypic and genotypic antimicrobial resistance profiles of campylobacter jejuni isolated from cattle, sheep, and free-range poultry faeces.minimum inhibitory concentrations (mic) of 13 antimicrobial agents were determined by broth microdilution for 72 campylobacter jejuni strains from livestock. twenty-three (31.9%) isolates were fully susceptible; all isolates were susceptible to erythromycin, chloramphenicol, streptomycin, gentamicin, sulfamethoxazole, and meropenem, and all but one to kanamycin. resistance to quinolones was highest (52.8%), reaching similar values among poultry, dairy cattle, and sheep, but lower in beef cattle. ...201020224816
high-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories.matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is suitable for high-throughput and rapid diagnostics at low costs and can be considered an alternative for conventional biochemical and molecular identification systems in a conventional microbiological laboratory. first, we evaluated maldi-tof ms using 327 clinical isolates previously cultured from patient materials and identified by conventional techniques (vitek-ii, api, and biochemical tests). discre ...201020053859
amoebae and algae can prolong the survival of campylobacter species in co-culture.several species of free-living amoebae can cause disease in humans. however, in addition to the direct pathogenicity of e.g. acanthamoebae and naegleria species, they are recognized as environmental hosts, indirectly involved in the epidemiology of many pathogenic bacteria. although several studies have demonstrated intracellular survival of many different bacteria in these species, the extent of such interactions as well as the implications for the epidemiology of the bacterial species involved ...201020056117
comparison of campylobacter populations isolated from a free-range broiler flock before and after slaughter.relatively little is known about the campylobacter genotypes colonizing extensively reared broiler flocks and their survival through the slaughter process, despite the increasing demand for free-range and organic products by the consumer. campylobacter isolates from a free-range boiler flock, sampled before and after slaughter, were genotyped by mlst (multilocus sequence typing) and sequence analysis of the flaa short variable region (svr). the campylobacter genotypes isolated before and after s ...201020071049
genetic similarity of campylobacter isolates in humans, food, and water sources in central poland.campylobacter spp. is an important cause of gastroenteritis in humans throughout the world. however, sources of these infections are often difficult to identify. therefore, this study aimed at analyzing the genetic relatedness of campylobacter isolates from environmental and food samples as well as stool specimens of diarrheal patients obtained in a single geographic region in poland. only a few campylobacter jejuni isolates (4/18, 22%) could be assigned to one cluster, whereas the majority were ...201020001328
method for bacteriophage isolation against target campylobacter strains.poultry meat is considered a major source of campylobacter. this micro-aerobic bacterium is commonly responsible for foodborne illness. this work focuses on the isolation of campylobacter coli lytic bacteriophages (phages) against target c. coli strains.201020002571
expanded multilocus sequence typing and comparative genomic hybridization of campylobacter coli isolates from multiple hosts.the purpose of this work was to evaluate the evolutionary history of campylobacter coli isolates derived from multiple host sources and to use microarray comparative genomic hybridization to assess whether there are particular genes comprising the dispensable portion of the genome that are more commonly associated with certain host species. genotyping and clonalframe analyses of an expanded 16-gene multilocus sequence typing (mlst) data set involving 85 isolates from 4 different hosts species te ...201020097815
characterization of two diesel fuel degrading microbial consortia enriched from a non acclimated, complex source of microorganisms.the bioremediation of soils impacted by diesel fuels is very often limited by the lack of indigenous microflora with the required broad substrate specificity. in such cases, the soil inoculation with cultures with the desired catabolic capabilities (bioaugmentation) is an essential option. the use of consortia of microorganisms obtained from rich sources of microbes (e.g., sludges, composts, manure) via enrichment (i.e., serial growth transfers) on the polluting hydrocarbons would provide biorem ...201020158909
a pulsed-field gel electrophoresis study of the genetic diversity of campylobacter jejuni and campylobacter coli in poultry flocks in slovenia.campylobacter jejuni and c. coli have recently become the most frequent cause of bacterial foodborne enteric infection in most industrialised countries. consumption and handling of undercooked contaminated poultry meat was identified as an important risk factor for human campylobacteriosis. the aim of this study was to ascertain the genetic diversity of c. jejuni and c. coli strains isolated from poultry in slovenia. a total of 68 isolates (42 c. jejuni , 26 c. coli ) from faeces (n = 48), meat ...201020159735
rapid detection and differentiation of campylobacter jejuni, campylobacter coli, and campylobacter lari in food, using multiplex real-time pcr.a multiplex real-time pcr assay based on four differently labeled taqman probes for detection and differentiation of the thermophilic campylobacter species c. jejuni, c. coli, and c. lari was established and validated in food products. this assay combines two previously published pcr assays for c. jejuni and c. coli with a newly developed detection assay for c. lari and an internal amplification control system. the selectivity of the method was determined by analyzing 70 campylobacter strains an ...201020132668
homopolymeric tracts represent a general regulatory mechanism in prokaryotes.while, traditionally, regulation of gene expression can be grouped into transcriptional, translational, and post-translational mechanisms, some mechanisms of rapid genetic variation can also contribute to regulation of gene expression, e.g., phase variation.201020144225
campylobacteriosis and other bacterial gastrointestinal diseases in sofia, bulgaria for the period 1987-2008.campylobacter is the most commonly reported enteric bacterial pathogen in humans. we still do not have any systematic data concerning campylobacteriosis in bulgaria. for that reason, we present data of the thermophilic campylobacter jejuni and campylobacter coli in the aetiology of diarrhoeal diseases in sofia, for the period from 1987 to 2008. the study included patients from 0 to over 65 years-old. a total of 51,607 faecal specimens were screened for campylobacter. c. jejuni and c. coli were d ...201020122383
proteomic analysis of iron acquisition, metabolic and regulatory responses of yersinia pestis to iron starvation.the gram-negative bacterium yersinia pestis is the causative agent of the bubonic plague. efficient iron acquisition systems are critical to the ability of y. pestis to infect, spread and grow in mammalian hosts, because iron is sequestered and is considered part of the innate host immune defence against invading pathogens. we used a proteomic approach to determine expression changes of iron uptake systems and intracellular consequences of iron deficiency in the y. pestis strain kim6+ at two phy ...201020113483
prevalence and antimicrobial resistance of campylobacter isolates in broilers from china.the prevalence and antimicrobial resistance of campylobacter spp. in broiler chickens were determined in shandong province, china. in total, 275 campylobacter isolates were obtained from 767 broiler cecal samples, including 208 campylobacter jejuni, 53 campylobacter coli, and 14 unidentified campylobacter isolates. minimal inhibitory concentrations of 11 antimicrobial agents were determined using the agar dilution method recommended by clsi. more than 98% of the tested campylobacter isolates wer ...201020116182
prevalence and characterization of campylobacter spp. isolated from domestic and imported poultry meat in korea, 2004–2008.campylobacteriosis in humans is primarily caused by handling or consuming contaminated poultry or their products. the aims of this study were to estimate the prevalence of campylobacter spp. in domestic and imported poultry meat in korea and to further characterize the obtained isolates. from 2004 to 2008, a total of 475 domestic and 867 imported raw poultry meat samples were examined for the presence of campylobacter spp. among 475 domestic poultry meat samples, campylobacter jejuni and campylo ...201020939742
occurrence of salmonella, campylobacter, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes in swine.the objective of this study was to investigate the occurrence of major bacterial foodborne pathogens in swine. in total, 359 samples from manure storage tanks (91) and fresh pooled faeces (268) obtained from finisher (110), sows (78) and weanlings (80) were collected and tested. campylobacter, salmonella, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes were isolated from 36.5%, 31.5%, 5.8%, 3.3% and 3.3% of samples respectively. all e. coli o157 isolates found on 10 far ...201019638163
pulsed field gel electrophoresis typing of human and retail foodstuff campylobacters: an irish perspective.campylobacter enteritis is a zoonosis, an infectious disease transmissible under normal conditions from vertebrate animals to man, presenting a major global public health burden. in this study, pulsed field gel electrophoresis (pfge) was employed to identify common genotypes in a collection of 600 campylobacter isolates in order to investigate if profiles obtained from retail samples of foodstuffs matched genotypes causing illness in the community in ireland. the campylobacters were isolated fro ...201021356447
fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of salmonella spp. in a broad spectrum of samples.a fluorescence in situ hybridization (fish) method for the rapid detection of salmonella spp. using a novel peptide nucleic acid (pna) probe was developed. the probe theoretical specificity and sensitivity were both 100%. the pna-fish method was optimized, and laboratory testing on representative strains from the salmonella genus subspecies and several related bacterial species confirmed the predicted theoretical values of specificity and sensitivity. the pna-fish method has been successfully ad ...201020453122
characterization of campylobacter jejuni and campylobacter coli strains isolated in the region of nis, serbia.campylobacter jejuni and campylobacter coli represent one of the main causes of bacterial diarrhoea in humans. although the disease is usually mild and self-limiting, severe chronic sequelae may occur, such as reactive arthritis, guillain-barré and miller fisher syndromes. serotyping is used as an epidemiological marker, while post-infective polyneuropathies are associated with several o serotypes.201021361150
evolution and population structure of salmonella enterica serovar newport.salmonellosis caused by salmonella enterica serovar newport is a major global public health concern, particularly because s. newport isolates that are resistant to multiple drugs (mdr), including third-generation cephalosporins (mdr-ampc phenotype), have been commonly isolated from food animals. we analyzed 384 s. newport isolates from various sources by a multilocus sequence typing (mlst) scheme to study the evolution and population structure of the serovar. these were compared to the populatio ...201020935094
ribosomal operon intergenic sequence region (isr) heterogeneity in campylobacter coli and campylobacter jejuni.the intergenic sequence regions (isr) between the 16s and 23s genes of campylobacter jejuni and campylobacter coli are markedly different for each species. however, in the genomic sequence for camp. coli rm2228, two rrna operons have an isr that is characteristic of camp. coli, and the third operon is characteristic of camp. jejuni. the aim of this study was to determine the prevalence of isr heterogeneity in these organisms.201020849393
role of the pjm1 plasmid-encoded transport proteins fatb, c and d in ferric anguibactin uptake in the fish pathogen vibrio anguillarum.vibrio anguillarum serotype o1 is part of the natural flora in the aquatic habitat, but under certain circumstances it can cause terminal haemorrhagic septicemia in marine and fresh water fish due to the action of the anguibactin iron uptake system encoded by the virulence plasmid pjm1. this plasmid harbours the genes for the biosynthesis of the siderophore anguibactin and the ferric anguibactin transport proteins fatd, c, b and a encoded in the iron transport operon. the fata protein is the out ...201021304833
mutations in the rpsl gene are involved in streptomycin resistance in campylobacter coli.to characterize the mechanisms of streptomycin (str) resistance in campylobacter coli, we chose 17 isolates that were resistant to str, erythromycin (ery), or both, and the putative str resistance target genes rpsl, rrs, and gidb were analyzed for mutations. the presence of the aade gene encoding aminoglycoside 6-adenylyltransferase was also evaluated. to reveal putative connection between ery and str resistance mechanisms, 13 c. coli isolates initially susceptible to str and ery were exposed to ...201020370506
influence of tetracycline resistance on the transport of manure-derived escherichia coli in saturated porous media.in this research, tetracycline resistant (tet(r)) and tetracycline susceptible (tet(s)) escherichia coli isolates were retrieved from dairy manure and the influence of tetracycline resistance on the transport of e. coli in saturated porous media was investigated through laboratory column transport experiments. experimental results showed that tet(r)e. coli strains had higher mobility than the tet(s) strains in saturated porous media. measurements of cell surface properties suggested that tet(r)e ...201021211813
influence of tetracycline resistance on the transport of manure-derived escherichia coli in saturated porous media.in this research, tetracycline resistant (tet(r)) and tetracycline susceptible (tet(s)) escherichia coli isolates were retrieved from dairy manure and the influence of tetracycline resistance on the transport of e. coli in saturated porous media was investigated through laboratory column transport experiments. experimental results showed that tet(r)e. coli strains had higher mobility than the tet(s) strains in saturated porous media. measurements of cell surface properties suggested that tet(r)e ...201021211813
generation of the pathogenic r5-tropic simian/human immunodeficiency virus shivad8 by serial passaging in rhesus macaques.a new pathogenic r5-tropic simian/human immunodeficiency virus (shiv) was generated following serial passaging in rhesus macaques. all 13 animals inoculated with shiv(ad8) passaged lineages experienced marked depletions of cd4(+) t cells. ten of these infected monkeys became normal progressors (nps) and had gradual losses of both memory and naïve cd4(+) t lymphocytes, generated antiviral cd4(+) and cd8(+) t cell responses, and sustained chronic immune activation while maintaining variable levels ...201020147396
differences in methylation at gatc sites in genomic dna of campylobacter coli from turkeys and swine.a significant fraction (46/108, 43%) of swine isolates of campylobacter coli but none of 81 isolates of c. coli from turkeys had genomic dna that was resistant to digestion by mboi, suggesting methylation of adenines at gatc sites. no consistent association was noted between antimicrobial resistance and mboi resistance. seven swine-associated multilocus sequence typing-based sequence types (sts) were detected among multiple isolates with mboi-resistant dna. the data suggest host-associated dna m ...201020833787
understanding the molecular epidemiology of the footrot pathogen dichelobacter nodosus to support control and eradication programs.the gram-negative anaerobe dichelobacter nodosus is the primary etiologic agent of ovine footrot. few studies of the genetic diversity and epidemiology of d. nodosus have been done, despite the economic cost and welfare implications of the disease. this study examined a large collection of australian isolates; 735 isolates from footrot-infected sheep from 247 farms in western australia (wa) were tested by pulsed-field gel electrophoresis (pfge), and a subset of 616 isolates was tested by infrequ ...201020071558
microbiological quality of blue mussels (mytilus edulis) in nunavik, quebec: a pilot study.this pilot study was aimed at documenting the presence of fecal indicators and enteric pathogens in blue mussels (mytilus edulis) from 6 communities in nunavik, quebec. one to four 2 kg samples of mussels were collected at low tide in each community. samples were investigated by enumeration methods for the fecal indicators enterococci, escherichia coli, f-specific coliphages, clostridium perfringens, and by molecular identification for the pathogens norovirus, salmonella spp., campylobacter jeju ...201021076488
understanding the molecular epidemiology of the footrot pathogen dichelobacter nodosus to support control and eradication programs.the gram-negative anaerobe dichelobacter nodosus is the primary etiologic agent of ovine footrot. few studies of the genetic diversity and epidemiology of d. nodosus have been done, despite the economic cost and welfare implications of the disease. this study examined a large collection of australian isolates; 735 isolates from footrot-infected sheep from 247 farms in western australia (wa) were tested by pulsed-field gel electrophoresis (pfge), and a subset of 616 isolates was tested by infrequ ...201020071558
independent evolution of the core and accessory gene sets in the genus neisseria: insights gained from the genome of neisseria lactamica isolate 020-06.the genus neisseria contains two important yet very different pathogens, n. meningitidis and n. gonorrhoeae, in addition to non-pathogenic species, of which n. lactamica is the best characterized. genomic comparisons of these three bacteria will provide insights into the mechanisms and evolution of pathogenesis in this group of organisms, which are applicable to understanding these processes more generally.201021092259
enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada.the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ...201021106791
enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada.the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ...201021106791
evaluation of salmonella-vectored campylobacter peptide epitopes for reduction of campylobacter jejuni in broiler chickens.campylobacter is a leading cause of bacterial gastroenteritis in humans and is often linked to contaminated poultry products. live salmonella vectors expressing three linear peptide epitopes from campylobacter proteins cj0113 (omp18/cjad), cj0982c (cjaa), and cj0420 (ace393) were administered to chicks by oral gavage on the day of hatch, and the chicks were challenged with campylobacter jejuni on day 21. all three candidate vaccines produced consistent humoral immune responses with high levels o ...201021177910
evaluation of salmonella-vectored campylobacter peptide epitopes for reduction of campylobacter jejuni in broiler chickens.campylobacter is a leading cause of bacterial gastroenteritis in humans and is often linked to contaminated poultry products. live salmonella vectors expressing three linear peptide epitopes from campylobacter proteins cj0113 (omp18/cjad), cj0982c (cjaa), and cj0420 (ace393) were administered to chicks by oral gavage on the day of hatch, and the chicks were challenged with campylobacter jejuni on day 21. all three candidate vaccines produced consistent humoral immune responses with high levels o ...201021177910
evolution of an agriculture-associated disease causing campylobacter coli clade: evidence from national surveillance data in scotland.the common zoonotic pathogen campylobacter coli is an important cause of bacterial gastroenteritis worldwide but its evolution is incompletely understood. using multilocus sequence type (mlst) data of 7 housekeeping genes from a national survey of campylobacter in scotland (2005/6), and a combined population genetic-phylogenetics approach, we investigated the evolutionary history of c. coli. genealogical reconstruction of isolates from clinical infection, farm animals and the environment, reveal ...201021179537
epidemiologic investigation of immune-mediated polyradiculoneuropathy among abattoir workers exposed to porcine brain.in october 2007, a cluster of patients experiencing a novel polyradiculoneuropathy was identified at a pork abattoir (plant a). patients worked in the primary carcass processing area (warm room); the majority processed severed heads (head-table). an investigation was initiated to determine risk factors for illness.201020333310
typing of campylobacter jejuni and campylobacter coli isolated from live broilers and retail broiler meat by flaa-rflp, mlst, pfge and rep-pcr.we analyzed 100 campylobacter spp. isolates (c. jejuni and c. coli) from grenada, puerto rico and alabama, which were collected from live broilers or retail broiler meat. we analyzed these isolates with four molecular typing methods: restriction fragment length polymorphism of the flaa gene (flaa-rflp), multilocus sequence typing (mlst), pulsed-field gel electrophoresis (pfge), and automated repetitive extragenic palindromic polymerase chain reaction (rep-pcr) using the diversilab system. all me ...201021130125
prevalence and risk factors associated with campylobacter infections in broiler flocks in shiraz, southern iran.campylobacter species are among the most common bacterial causes of human gastroenteritis in many countries, and poultry meat is considered as a major source of human campylobacteriosis. the present study was conducted to determine the prevalence of infection by campylobacter jejuni and campylobacter coli in broiler flocks in shiraz and to investigate the possible risk factors for the campylobacter infections in this area. for detection of campylobacter, multiplex polymerase chain reaction (mpcr ...201021131089
prevalence and antimicrobial resistance profile of campylobacter spp. isolated from conventional and antimicrobial-free swine production systems from different u.s. regions.we conducted a study to compare the prevalence and antimicrobial resistance profile of campylobacter isolated from 34 farm-slaughter pair cohorts of pigs raised in conventional and antimicrobial-free (abf) production systems. isolates originated from four different states of two geographic regions (region 1--ohio and michigan; region 2--wisconsin and iowa). a total of 838 fecal and 1173 carcass samples were examined. campylobacter isolates were speciated using multiplex polymerase chain reaction ...201021133777
in vitro activity and single-step mutational analysis of rifamycin sv tested against enteropathogens associated with traveler's diarrhea and clostridium difficile.rifamycin sv is a broad-spectrum, poorly absorbed antimicrobial agent that, when coupled with mmx technology, is being targeted for the oral treatment of traveler's diarrhea (td) and clostridium difficile-associated disease (cdad). rifamycin sv was tested for activity against 911 td-associated enteropathogens and 30 c. difficile isolates collected from several global surveillance studies. rifamycin sv demonstrated similar antimicrobial activity levels against the enterobacteriaceae, with mic₅₀ v ...201021149623
in vitro activity and single-step mutational analysis of rifamycin sv tested against enteropathogens associated with traveler's diarrhea and clostridium difficile.rifamycin sv is a broad-spectrum, poorly absorbed antimicrobial agent that, when coupled with mmx technology, is being targeted for the oral treatment of traveler's diarrhea (td) and clostridium difficile-associated disease (cdad). rifamycin sv was tested for activity against 911 td-associated enteropathogens and 30 c. difficile isolates collected from several global surveillance studies. rifamycin sv demonstrated similar antimicrobial activity levels against the enterobacteriaceae, with mic₅₀ v ...201021149623
drinking water as the source of campylobacter coli infection in grandparent heavy breeders.the aim of the present study was the molecular identification of a common source of infection of campylobacter coli in two grandparent breeder farms. campylobacter jejuni and c. coli were isolated from well water and cloacal swabs from grandparent chickens. colonies were genotyped using restriction fragment length polymorphism-flaa gene, pulsed field gel electrophoresis and multi-locus sequence typing. the same genotype of c. coli was found in both farms and in the well from which drinking water ...201021154058
molecular characterization and environmental mapping of campylobacter isolates in a subset of intensive poultry flocks in ireland.irish breeder and intensive broiler flocks together with the corresponding poultry farm environment were sampled for the presence of campylobacter with the aim of identifying potential sources and transmission routes of poultry flock contamination. the genetic diversity of a subset of campylobacter isolates was examined by analysis of the flaa-short variable region (svr). additional discrimination for a further subset of these isolates was achieved using multilocus sequence typing (mlst) analysi ...201021039133
detection of gyra mutation among clinical isolates of campylobacter jejuni isolated in egypt by mama-pcr.campylobacter spp are the major cause of enteritis in humans and more than 90% of reported infections are caused by campylobacter jejuni. fluoroquinolones such as ciprofloxacin are the antibiotics of choice for treatment. an increase in the frequency of ciprofloxacin-resistant campylobacter has been reported globally due to a single base mutation (c-257 to t) in codon 86 of the quinolone resistance determining region (qrdr) of the gyra gene altering the amino acid sequence from threonine at posi ...201021045366
campylobacter jejuni and campylobacter coli genotyping by high-resolution melting analysis of a flaa fragment.the highly variable flagellin-encoding flaa gene has long been used for genotyping campylobacter jejuni and campylobacter coli. high-resolution melting (hrm) analysis is emerging as an efficient and robust method for discriminating dna sequence variants. the objective of this study was to apply hrm analysis to flaa-based genotyping. the initial aim was to identify a suitable flaa fragment. it was found that the pcr primers commonly used to amplify the flaa short variable repeat (svr) yielded a m ...201019933351
antimicrobial resistance of campylobacter isolates from retail meat in the united states between 2002 and 2007.the emergence of antimicrobial resistance in campylobacter spp. has been a growing public health concern globally. the objectives of this study were to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of campylobacter spp. recovered by the national antimicrobial resistance monitoring system (narms) retail meat program. retail meat samples (n = 24,566) from 10 u.s. states collected between 2002 and 2007, consisting of 6,138 chicken breast, 6,109 ground turkey, 6,171 ...201020971875
characterization of erythromycin-resistant porcine isolates of campylobacter coli.erythromycin-resistant campylobacter organisms were isolated from swine, and their resistance to the antibiotic was characterized. one hundred fourteen campylobacter organisms were isolated from 572 swine intestinal samples. all isolates were identified as campylobacter coli by sequence analysis of 16s rrna gene and polymerase chain reactions with primers specific to hippuricase gene in campylobacter jejuni and aspartokinase gene in c. coli. minimal inhibitory concentrations (mics) of erythromyc ...201020735175
obstacles of multiplex real-time pcr for bacterial 16s rdna: primer specifity and dna decontamination of taq polymerase.background: the detection of a broad range of bacteria by pcr is applied for the screening of blood and blood products with special attention to platelet concentrates. for practical use it is desirable that detection systems include gram-positive, gram-negative and non-gram-stainable bacteria. it is quite challenging to achieve high sensitivity along with a clear negative control with pcr reagents, because especially taq polymerase is contaminated with traces of bacterial dna. methods: bacterial ...201020737013
the glycerophospho metabolome and its influence on amino acid homeostasis revealed by brain metabolomics of gde1(-/-) mice.gde1 is a mammalian glycerophosphodiesterase (gde) implicated by in vitro studies in the regulation of glycerophophoinositol (gropins) and possibly other glycerophospho (grop) metabolites. here, we show using untargeted metabolomics that gropins is profoundly (>20-fold) elevated in brain tissue from gde1(-/-) mice. furthermore, two additional grop metabolites not previously identified in eukaryotic cells, glycerophosphoserine (gropser) and glycerophosphoglycerate (gropgate), were also highly ele ...201020797612
the in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of campylobacter coli and campylobacter jejuni in chickens.poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. campylobacter coli and campylobacter jejuni are the most common campylobacter species associated with this disease. these pathogens live in the intestinal tract of most avian species and under commercial conditions they spread rapidly to infect a high proportion of the flock, which makes their treatment and prevention very difficult. bacteriophages (phag ...201020809975
comparison of premier campy enzyme immunoassay (eia), prospect campylobacter eia, and immunocard stat! campy tests with culture for laboratory diagnosis of campylobacter enteric infections.campylobacter enteritis is a food-borne or waterborne illness caused almost exclusively by campylobacter jejuni and, to a lesser extent, by campylobacter coli. these organisms produce indistinguishable clinical diseases and together represent the second most common cause of bacterial diarrhea in the united states and the leading cause of enteric infection throughout the world. the conventional approach to the laboratory diagnosis of campylobacter enteritis is based on the recovery of the organis ...201020810765
fragmentation of the large subunit ribosomal rna gene in oyster mitochondrial genomes.discontinuous genes have been observed in bacteria, archaea, and eukaryotic nuclei, mitochondria and chloroplasts. gene discontinuity occurs in multiple forms: the two most frequent forms result from introns that are spliced out of the rna and the resulting exons are spliced together to form a single transcript, and fragmented gene transcripts that are not covalently attached post-transcriptionally. within the past few years, fragmented ribosomal rna (rrna) genes have been discovered in bilatera ...201020813041
prevalence of campylobacter jejuni, campylobacter coli and enteric helicobacter in domestic and free living birds in north-western italy.in order to investigate the prevalence of some thermophilic campylobacter (c. jejuni and c. coli) and enteric helicobacter (h. pullorum and h. canadensis) in domestic and wild birds, a total of 278 bird caecal samples were analyzed over a 2 year period in north-western italy. samples were collected from poultry raised in intensive farming at the slaughterhouse (n=102, group a) and in small scale rural farms (n=60, group b) as well as from wild birds (n=116, group c). pcr amplifications were carr ...201020814861
prevalence, antibiograms, and transferable tet(o) plasmid of campylobacter jejuni and campylobacter coli isolated from raw chicken, pork, and human clinical cases in korea.the antibiotic resistance patterns and prevalence of the transferable tet(o) plasmid were investigated in campylobacter jejuni and campylobacter coli isolates from raw chicken, pork, and humans with clinical campylobacteriosis. a total of 180 c. jejuni and c. coli isolates were identified, and the prevalence rates of c. jejuni and c. coli in raw chicken samples were 83% (83 of 100) and 73% (73 of 100), respectively. twelve percent (6 of 50) and 10% (5 of 50) of pork samples were contaminated wit ...201020819352
survival of campylobacter jejuni and campylobacter coli on retail broiler meat stored at -20, 4, or 12 degrees c and development of weibull models for survival.survival of campylobacter jejuni and campylobacter coli isolated from broiler meat was investigated and modeled on retail breast meat. meat portions were inoculated with c. jejuni or c. coli at 6.4 to 6.8 log cfu/g followed by storage at -20 degrees c for 84 days or at 4 or 12 degrees c for 14 days. kinetic data within a species and temperature were fitted to the weibull model. when >or=70% of the residuals were in an acceptable prediction zone from -1 (fail-safe) to 0.5 (fail-dangerous) log uni ...201020819353
prevalence and antimicrobial resistance of campylobacter isolates from humans and chickens in bahir dar, ethiopia.in this study, the isolation and antimicrobial resistance of campylobacter jejuni and campylobacter coli strains from chickens and humans in bahir dar, ethiopia, were analyzed. two hundred and ten human and 220 chicken samples were analyzed between october 2007 and april 2008. seventeen human and 160 chicken campylobacter species were isolated. the overall prevalence of thermophilic campylobacters was 8% and 72.7% in humans and chickens, respectively. in humans, 94.1% of the isolates were c. jej ...201020482228
genotyping and pcr detection of potential virulence genes in campylobacter jejuni and campylobacter coli isolates from different sources in poland.the prevalence of potential virulence markers was determined among the population of polish campylobacter jejuni and campylobacter coli isolates from children, chickens, pigs and dogs. the presence of the flaa, flab, cdta, cdtb, cdtc, cdtabc, virb11, and cj0588 genes among 74 c. jejuni and 15 c. coli isolates was detected by pcr. high prevalence of five different putative virulence and toxin genes (flaa, cdta, cdtb, cdtc, and cj0588) was found among isolates obtained from children, chickens and ...201020490760
repeated therapeutic dosing selects macrolide-resistant campylobacter spp. in a turkey facility.this study assessed the effects of the therapeutic use of tylan® in a large-scale turkey production facility on the selection of macrolide-resistant campylobacter.201020497488
fluoroquinolone resistance detection in campylobacter coli and campylobacter jejuni by luminex xmap technology.the proportion of campylobacter spp. isolates that are resistant to fluoroquinolones, the drugs of choice for campylobacteriosis, has been increasing worldwide. we developed an innovative method based on a luminex xmap dna suspension array that allows the identification of campylobacter species and, simultaneously, the detection of the most common point mutation in the gyra gene (substitution from threonine 86 to isoleucine 86) that is responsible for fluoroquinolone resistance. ninety-six campy ...201020500084
prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from cattle between 2002 and 2006 in france.feces from 2,255 cattle (calves, young beef cattle, and culled cows) were collected at slaughter from nine departments across france. campylobacter was recovered from 16.5% of the 2,255 samples (c. jejuni from 12.8% and c. coli from 3.7%), predominantly from calves. antimicrobial resistance to six antibiotics of medical and/or veterinary interest was tested with the e-test. resistance to tetracycline was found in most isolates (52.8% of c. jejuni isolates and 88.1% of c. coli isolates) in contra ...201020501032
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1501 - 1600 of 2341