Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| prevalence and risk factors for thermotolerant species of campylobacter in poultry meat at retail in europe. | the thermotolerant species campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis are the causative agents of the human illness called campylobacteriosis. this infection represents a threat for the health of consumers in europe. it is well known that poultry meat is an important food vehicle of campylobacter infection. as emerged from the reported scientific literature published between 2006 and 2016, poultry meat sold at retail level in europe represents an i ... | 2017 | 28854745 |
| isolation and identification of campylobacter spp. from poultry and poultry by-products in tunisia by conventional culture method and multiplex real-time pcr. | thermophilic campylobacter spp. are one of the primary causes of bacterial human diarrhea. the consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. the aims of this study were to determine the prevalence of thermophilic campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm campylobacter jejuni and campylobacter coli isolates by using the multiplex pcr assay. two hundred fifty fre ... | 2017 | 28853632 |
| genome reduction for niche association in campylobacter hepaticus, a cause of spotty liver disease in poultry. | the term "spotty liver disease" (sld) has been used since the late 1990s for a condition seen in the uk and australia that primarily affects free range laying hens around peak lay, causing acute mortality and a fall in egg production. a novel thermophilic sld-associated campylobacter was reported in the united kingdom (uk) in 2015. subsequently, similar isolates occurring in australia were formally described as a new species, campylobacter hepaticus. we describe the comparative genomics of 10 c. ... | 2017 | 28848714 |
| fecal shedding of campylobacter jejuni and campylobacter coli among feral pigs in texas. | the population and range of feral pigs in the united states are rapidly expanding, yet key knowledge gaps exist regarding their role in the ecology and transmission of foodborne pathogens. our objectives were to estimate the prevalence of campylobacter jejuni and campylobacter coli shedding among feral pigs throughout texas and to identify risk factors for positive status. faecal samples were collected from feral pigs in texas from february 2014 through may 2015, and target organisms were detect ... | 2017 | 28834190 |
| survival of selected foodborne pathogens on dry cured pork loins. | the safety of ready-to-eat products such as cured pork loins must be guaranteed by the food industry. in the present study, the efficacy of the dry curing process of pork loins obtained from free-range pigs in the reduction of three of the most important foodborne pathogens is analysed. a total of 28 pork loin segments, with an average weight of 0.57±0.12kg, were divided into four groups with three being inoculated by immersion with 7logcfu/ml of either salmonella typhimurium, campylobacter coli ... | 2017 | 28759797 |
| cardamom, cumin, and dill weed essential oils: chemical compositions, antimicrobial activities, and mechanisms of action against campylobacter spp. | natural antimicrobials as well as essential oils (eos) have gained interest to inhibit pathogenic microorganisms and to control food borne diseases. campylobacter spp. are one of the most common causative agents of gastroenteritis. in this study, cardamom, cumin, and dill weed eos were evaluated for their antibacterial activities against campylobacter jejuni and campylobacter coli by using agar-well diffusion and broth microdilution methods, along with the mechanisms of antimicrobial action. che ... | 2017 | 28714890 |
| genetic diversity of thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. | the objective of this study was to investigate a clonal relationship among thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. a total of 128 thermotolerant campylobacter spp. (89 c. jejuni and 39 c. coli) isolates from six poultry meat chains were examined. these isolates were from: a) hens from breeder flocks, b) chickens on the farm (at ages 1 wk and 5 wk), c) chicken carcasses in the slaughterhouse, and d) chicken carcasses in the r ... | 2017 | 28712509 |
| no clear differences between organic or conventional pig farms in the genetic diversity or virulence of campylobacter coli isolates. | to evaluate the impact of pig farm management on the genetic diversity and on the virulence of campylobacter coli, we characterized isolates from 19 organic pig farms (62 isolates) and from 24 conventional pig farms (58 isolates). the 120 c. coli isolates were typed using pulsed field gel electrophoresis (pfge) and multilocus sequence typing (mlst) and the presence of nine virulence genes was screened using real-time pcr. the capacity of adhesion and invasion of 61 isolates (32 from organic and ... | 2017 | 28694791 |
| a study on campylobacter jejuni and campylobacter coli through commercial broiler production chains in thailand: antimicrobial resistance, the characterization of dna gyrase subunit a mutation, and genetic diversity by flagellin a gene restriction fragment length polymorphism. | contaminated poultry meat is regarded as the main source of human campylobacteriosis. during september 2014 and february 2015, breeder flocks, hatcheries, and broiler farms from two chicken production chains were investigated chronologically. five commercial breeder flocks (breeder flocks 1-5), two hatcheries (hatcheries a and b), and five broiler flocks (broiler flocks 1-5) were sampled in this study. campylobacter colonization of both breeder and broiler flocks was determined from cloacal swab ... | 2017 | 28665716 |
| campylobacter species isolated from pigs in grenada exhibited novel clones: genotypes and antimicrobial resistance profiles of sequence types. | infections caused by campylobacter species pose a severe threat to public health worldwide. however, in grenada, the occurrence and characteristics of campylobacter in food animals, including pigs, remain mostly unknown. in this study, we identified the sequence types (sts) of campylobacter from young healthy pigs in grenada and compared the results with previous studies in grenada and other countries. antimicrobial resistance patterns and diversity of the campylobacter clones were evaluated. ni ... | 2017 | 28650672 |
| impact of built-up-litter and commercial antimicrobials on salmonella and campylobacter contamination of broiler carcasses processed at a pilot mobile poultry-processing unit. | the small-scale mobile poultry-processing unit (mppu) produced raw poultry products are of particular food safety concern due to exemption of usda poultry products inspection act. limited studies reported the microbial quality and safety of mppu-processed poultry carcasses. this study evaluated the salmonella and campylobacter prevalence in broiler ceca and on mppu-processed carcasses and efficacy of commercial antimicrobials against campylobacter jejuni on broilers. in study i, straight-run hub ... | 2017 | 28649571 |
| campylobacter coli in organic and conventional pig production in france and sweden: prevalence and antimicrobial resistance. | the purpose of the study was to evaluate and compare the prevalence and antimicrobial resistance of campylobacter coli in conventional and organic pigs from france and sweden. fecal or colon samples were collected at farms or at slaughterhouses and cultured for campylobacter. the minimum inhibitory concentrations of ciprofloxacin, nalidixic acid, streptomycin, tetracycline, erythromycin, and gentamicin were determined by microdilution for a total of 263 french strains from 114 pigs from 50 diffe ... | 2017 | 28611754 |
| irrigation water sources and time intervals as variables on the presence of campylobacter spp. and listeria monocytogenes on romaine lettuce grown in muck soil. | irrigation water has been identified as a possible source of vegetable contamination by foodborne pathogens. risk management for pathogens such as campylobacter spp. and listeria monocytogenes in fields can be influenced by the source of the irrigation water and the time interval between last irrigation and harvest. plots of romaine lettuce were irrigated with manure-contaminated water or aerated pond water 21, 7, or 3 days prior to harvesting, and water and muck soil samples were collected at e ... | 2017 | 28604176 |
| detection and prevalence of antimicrobial resistance genes in <i>campylobacter</i> spp. isolated from chickens and humans. | campylobacter spp. are common pathogenic bacteria in both veterinary and human medicine. infections caused by campylobacter spp. are usually treated using antibiotics. however, the injudicious use of antibiotics has been proven to spearhead the emergence of antibiotic resistance. the purpose of this study was to detect the prevalence of antibiotic resistance genes in campylobacter spp. isolated from chickens and human clinical cases in south africa. one hundred and sixty one isolates of campylob ... | 2017 | 28582978 |
| next generation sequencing-based multigene panel for high throughput detection of food-borne pathogens. | contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. bacteria that cause foodborne diseases are known as foodborne pathogens (fbps). accurate detection methods that are able to reveal the presence of fbps in food matrices are in constant demand, in order to ensure safe foods wi ... | 2017 | 28578266 |
| dissemination of erm(b) and its associated multidrug-resistance genomic islands in campylobacter from 2013 to 2015. | a total of 1372 campylobacter isolates (1107 campylobacter coli and 265 campylobacter jejuni) were obtained from 3462 samples collected from slaughterhouses and farms in three representative regions of china (shandong, guangdong, and shanghai) over three successive years (2013-2015). of these, 84 (84/1372, 6.1%) were erm(b)-positive, and all 84 positive isolates were identified as c. coli (83 chicken isolates and one swine isolate). the prevalence of erm(b) in campylobacter isolates was compared ... | 2017 | 28532801 |
| monitoring of virulence genes, drug-resistance in campylobacter coli isolated from golden retrievers. | the investigation was performed on 75 of golden retriever puppies. faecal samples were collected on the 42 day of the puppies life (control). probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppies life). all isolates of campylobacter coli isolated prior to the administration of the probiotic were found to contain the cadf gene responsible for adhesion, as well as, the flaa ... | 2016 | 28520333 |
| prevalence of campylobacter species in wild birds of south korea. | campylobacter species cause human gastrointestinal infections worldwide. they commonly inhabit intestines of avian species including wild birds. they might play a role in the spread of infections to humans and other bird species. the prevalence of campylobacter species in 2164 faecal samples of wild birds (representing 71 species and 28 families) captured across the korean peninsula was evaluated in this study. the overall prevalence was 15.3% (332/2164). bird species belonging to the family cha ... | 2017 | 28503965 |
| novel sampling method for assessing human-pathogen interactions in the natural environment using boot socks and citizen scientists, with application to campylobacter seasonality. | this paper introduces a novel method for sampling pathogens in natural environments. it uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). this sampling method is implemented using a citizen science approach: groups of three walkers wearing boot socks undertook one of six routes, 40 times over 16 months in the north west ... | 2017 | 28500040 |
| identification and differentiation of campylobacter species by high-resolution melting curve analysis. | campylobacter jejuni and campylobacter coli are the important food-born zoonotic pathogen, also are leading causes of human food borne illnesses worldwide. cadf gene is expressed in all c. jejuni and c. coli strains and mediates cell binding to the cell matrix protein, fibronectin. high-resolution melting (hrm) analysis is emerging as an efficient and robust method for discriminating dna sequence variants. the goal of this study was to apply hrm analysis to identification of c. jejuni and c. col ... | 2017 | 28478199 |
| simultaneous identification of campylobacter jejuni, campylobacter coli, and campylobacter lari with smartcycler-based multiplex quantitative polymerase chain reaction. | consumption of campylobacter contaminated food or water is a leading cause of human acute gastroenteritis. campylobacter jejuni, campylobacter coli, and campylobacter lari account for over 95% of total campylobacter infections. a multiplex quantitative polymerase chain reaction (qpcr) for simultaneous identification of c. jejuni, c. coli, and c. lari was developed for use with the smartcycler ii system. | 2017 | 28440688 |
| lauric acid as feed additive - an approach to reducing campylobacter spp. in broiler meat. | the increasing prevalence of campylobacter spp. within broiler populations is a major problem for food safety and consumer protection worldwide. in vitro studies could already demonstrate that campylobacter spp. are susceptible to lauric acid. the purpose of this study was to examine in vivo the influence of lauric acid as a feed additive on slaughter parameters, muscle fatty acid profile, meat quality traits and the reduction of campylobacter coli in inoculated meat of ross 308 (r308) and hubba ... | 2017 | 28419122 |
| postchill antimicrobial treatments to control salmonella, listeria, and campylobacter contamination on chicken skin used in ground chicken. | ground poultry products are frequently contaminated with foodborne pathogens. with the potential for increased regulatory scrutiny, it is important to use sufficient intervention strategies to control pathogen levels effectively. a large proportion of the bacteria introduced to ground chicken are likely to come from broiler skin, which is added to achieve target fat content and maintain product texture and taste. in this research, antimicrobials, including 50 ppm of chlorine and 1,200 ppm of per ... | 2017 | 28414258 |
| the current state of macrolide resistance in campylobacter spp.: trends and impacts of resistance mechanisms. | campylobacter spp., especially campylobacter jejuni and c. coli, are leading bacterial foodborne pathogens worldwide. in the united states, an estimated 0.8 million cases of campylobacteriosis occur annually, mostly involving c. jejuni campylobacteriosis is generally self-limiting, but in severe cases, treatment with antibiotics may be mandated. the increasing incidence of fluoroquinolone resistance in campylobacter has rendered macrolides such as erythromycin and azithromycin the drugs of choic ... | 2017 | 28411226 |
| characterisation of aerotolerant forms of a robust chicken colonizing campylobacter coli. | campylobacter contaminated poultry meat is a major source of human foodborne illness. campylobacter coli strain or12 is a robust colonizer of chickens that was previously shown to outcompete and displace other campylobacter strains from the chicken's gastrointestinal tract. this strain is capable of aerobic growth on blood agar. serial aerobic passage increased this aerotolerance as assessed by quantitative assays for growth and survival on solid media. aerotolerance was also associated with inc ... | 2017 | 28396658 |
| prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from children and environmental sources in urban and suburban areas. | campylobacteriosis is a dominant bacterial cause of foodborne infection and is considered the main public health problem in europe and many other countries worldwide. in the study lasting from 2011 to 2013 we compared the prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from children, domestic animals, poultry meat and surface water in northern poland. | 2017 | 28376713 |
| faecal campylobacter shedding among dogs in animal shelters across texas. | epidemiologic studies on faecal campylobacter shedding among dogs in the united states have been limited, despite evidence that the incidence of human campylobacteriosis has increased over the last decade. our objectives were to estimate the prevalence of faecal campylobacter shedding among shelter dogs in texas, to estimate the specific prevalence of campylobacter jejuni and campylobacter coli shedding, and to identify risk factors for campylobacter-positive status. using a cross-sectional stud ... | 2017 | 28371319 |
| prevalence, phenotypic and genetic diversity of campylobacter in poultry fresh meat and poultry products on retail sale in tuscany (italy). | in this study, the prevalence of campylobacter spp. in poultry fresh meat and ready‑to‑cook products was evaluated. seventy‑three samples were collected at retail level from supermarkets and discount stores, obtaining 61.6% positivity. of 133 campylobacter isolates, 86 strains (campylobacter coli, 58.1% and campylobacter jejuni, 41.9%) were selected for characterisation on the basis of their smai and kpni pulsed field gel‑electrophoresis (pfge) profiles, to exclude clonal replicates. campylobact ... | 2017 | 28365923 |
| further characterization and independent validation of a dna aptamer-quantum dot-based magnetic sandwich assay for campylobacter. | previously reported dna aptamers developed against surface proteins extracted from campylobacter jejuni were further characterized by aptamer-based western blotting and shown to bind epitopes on proteins weighing ~16 and 60 kd from reduced c. jejuni and campylobacter coli lysates. proteins of these approximate weights have also been identified in traditional antibody-based western blots of campylobacter spp. specificity of the capture and reporter aptamers from the previous report was further va ... | 2017 | 28342148 |
| prevalence, antimicrobial resistance and genetic diversity of campylobacter coli and campylobacter jejuni in ecuadorian broilers at slaughter age. | thermotolerant campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. the linkage of human campylobacteriosis and poultry has been widely described. in this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of c. coli and c. jejuni in broilers from ecuador. caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of pichincha durin ... | 2017 | 28339716 |
| a quantitative real-time pcr approach for assessing campylobacter jejuni and campylobacter coli colonization in broiler herds. | human campylobacteriosis is a major public health concern in developed countries, with campylobacter jejuni and campylobacter coli from poultry recognized as the main source of human infection. identification of campylobacter-positive broiler herds before slaughter is essential for implementing measures to avoid carryover of pathogens via the slaughter process into the food chain. however, appropriate methods that have been validated for testing poultry flocks antemortem are lacking for campylob ... | 2017 | 28282226 |
| high prevalence and predominance of the aph(2″)-if gene conferring aminoglycoside resistance in campylobacter. | campylobacter is a major foodborne pathogen and previous studies revealed that campylobacter isolates from food-producing animals are increasingly resistant to gentamicin in china. the molecular epidemiology and genetic mechanisms responsible for gentamicin resistance in china have not been well understood. in this study, 607 campylobacter isolates of chicken and swine origins collected in the year of 2014 were analyzed, revealing that 15.6% (25/160) of the c. jejuni isolates and 79.9% (357/447) ... | 2017 | 28264854 |
| molecular typing of campylobacter jejuni and campylobacter coli isolated from various retail meats by mlst and pfge. | campylobacter species are one of the leading causes of foodborne disease in the united states. campylobacter jejuni and campylobacter coli are the two main species of concern to human health and cause approximately 95% of human infections. molecular typing methods, such as pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) are often used to source track foodborne bacterial pathogens. the aim of the present study was to compare pfge and mlst in typing strains of c. jeju ... | 2014 | 28234305 |
| variation in campylobacter multilocus sequence typing subtypes from chickens as detected on three plating media. | the objective of this study was to compare subtypes of campylobacter jejuni and campylobacter coli detected on three selective campylobacter plating media to determine whether each medium selected for different subtypes. fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. campylobacter was cultured and isolated from cecal contents and carcass rinses on campy-cefex, campy line, and rf campylobacter jejuni/coli ag ... | 2016 | 28221919 |
| whole genome sequencing and analysis of campylobacter coli yh502 from retail chicken reveals a plasmid-borne type vi secretion system. | campylobacter is a major cause of foodborne illnesses worldwide. campylobacter infections, commonly caused by ingestion of undercooked poultry and meat products, can lead to gastroenteritis and chronic reactive arthritis in humans. whole genome sequencing (wgs) is a powerful technology that provides comprehensive genetic information about bacteria and is increasingly being applied to study foodborne pathogens: e.g., evolution, epidemiology/outbreak investigation, and detection. herein we report ... | 2017 | 28217442 |
| temporal induction of pro-inflammatory and regulatory cytokines in human peripheral blood mononuclear cells by campylobacter jejuni and campylobacter coli. | campylobacter jejuni along with c. coli are major cause of human gastroenteritis worldwide. so far, the human immune response against campylobacter is not entirely clear. we hypothesize that it is coordinated by an interaction between pro-inflammatory and regulatory cytokines which is influenced by bacterial and host-individual differences. accordingly, we used peripheral blood mononuclear cells (pbmc) from healthy donors to study the primary systemic immune response to c. jejuni and c. coli. pb ... | 2017 | 28196097 |
| genetic diversity and antimicrobial resistance profiles of campylobacter coli and campylobacter jejuni isolated from broiler chicken in farms and at time of slaughter in central italy. | genetic diversity and antimicrobial resistance of campylobacter coli and campylobacter jejuni were investigated along the broiler chicken production chain in central italy. | 2017 | 28194914 |
| emergence of a plasmid-borne multidrug resistance gene cfr(c) in foodborne pathogen campylobacter. | to identify and characterize a novel cfr variant that recently emerged and confers multidrug resistance in campylobacter , a major foodborne pathogen. | 2017 | 28186558 |
| restriction glycosylases: involvement of endonuclease activities in the restriction process. | all restriction enzymes examined are phosphodiesterases generating 3΄-oh and 5΄-p ends, but one restriction enzyme (restriction glycosylase) excises unmethylated bases from its recognition sequence. whether its restriction activity involves endonucleolytic cleavage remains unclear. one report on this enzyme, r.pabi from a hyperthermophile, ascribed the breakage to high temperature while another showed its weak ap lyase activity generates atypical ends. here, we addressed this issue in mesophiles ... | 2017 | 28180312 |
| isolation, identification and differentiation of campylobacter spp. using multiplex pcr assay from goats in khartoum state, sudan. | the aim of this study was to identify and characterize thermophilic campylobacter species in faecal samples from goats in khartoum state, sudan, by application of multiplex polymerase chain reaction. campylobacteriosis is a zoonotic disease of global concern, and the organisms can be transmitted to human via food, water and through contact with farm animals and pets. there are five clinically related campylobacter species: campylobacter jejuni (c. jejuni). campylobacter coli, campylobacter lari, ... | 2017 | 28160162 |
| reply to "activity of fosfomycin on clinical isolates of campylobacter jejuni and campylobacter coli of enteric origin". | 2017 | 28119318 | |
| activity of fosfomycin on clinical isolates of campylobacter jejuni and campylobacter coli of enteric origin. | 2017 | 28119317 | |
| prevalence of thermotolerant campylobacter spp. in chicken meat in croatia and multilocus sequence typing of a small subset of campylobacter jejuni and campylobacter coli isolates. | in order to detect thermotolerant campylobacter spp., 241 samples of fresh chicken meat, at retail in croatia, were analysed according to a standard method, followed by biochemical test and molecular polymerase chain reaction/restriction enzyme analysis for exact species determination. campylobacter spp. prevalence was 73.86%. campylobacter jejuni and campylobacter coli were isolated from 53.53 and 15.35% of the samples, respectively. in 4.98% of isolates thermotolerant campylobacter spp. were n ... | 2016 | 28115906 |
| campylobacter coli infection in pet birds in southern italy. | avian species are considered as the main reservoir of campylobacter spp. however, few data are available on the presence of this microorganism in pet birds. this study was therefore performed to determine the prevalence of campylobacter spp. in pet birds bred in southern italy. faecal samples were collected from 88 cages housing different species of pet birds and examined by bacteriological culture and polymerase chain reaction. a total of 13.6% of the cage samples were positive for campylobacte ... | 2017 | 28061877 |
| first case report of myopericarditis linked to campylobacter coli enterocolitis. | campylobacter spp. are a common cause of mostly self-limiting enterocolitis. although rare, pericarditis and myopericarditis have been increasingly documented as complications following campylobacteriosis. such cases have occurred predominantly in younger males, and involved a single causative species, namely campylobacter jejuni. we report the first case of myopericarditis following campylobacter coli enterocolitis, with illness occurring in an immunocompetent middle-aged female. | 2017 | 28056838 |
| bacteriophage-based tools: recent advances and novel applications. | bacteriophages (phages) are viruses that infect bacterial hosts, and since their discovery over a century ago they have been primarily exploited to control bacterial populations and to serve as tools in molecular biology. in this commentary, we highlight recent diverse advances in the field of phage research, going beyond bacterial control using whole phage, to areas including biocontrol using phage-derived enzybiotics, diagnostics, drug discovery, novel drug delivery systems and bionanotechnolo ... | 2016 | 27990274 |
| restriction glycosylases: involvement of endonuclease activities in the restriction process. | all restriction enzymes examined are phosphodiesterases generating 3'-oh and 5'-p ends, but one restriction enzyme (restriction glycosylase) excises unmethylated bases from its recognition sequence. whether its restriction activity involves endonucleolytic cleavage remains unclear. one report on this enzyme, r.pabi from a hyperthermophile, ascribed the breakage to high temperature while another showed its weak ap lyase activity generates atypical ends. here, we addressed this issue in mesophiles ... | 2016 | 27980098 |
| systematic site-directed mutagenesis of the helicobacter pylori cagl protein of the cag type iv secretion system identifies novel functional domains. | the cag type iv secretion system, which contributes to inflammation and cancerogenesis during chronic infection, is one of the major virulence factors of the bacterial gastric pathogen helicobacter pylori. we have generated and characterized a series of non-marked site-directed chromosomal mutants in h. pylori to define domains of unknown function of the essential tip protein cagl of the cag secretion system. characterizing the cagl mutants, we determined that their function to activate cells an ... | 2016 | 27922023 |
| a current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens. | gastroenteritis is caused by a wide range of viral, bacterial and parasitic pathogens and causes millions of deaths worldwide each year, particularly in infant populations in developing countries. traditional microbiological culture and immunological based tests are time consuming, laborious and often lack diagnostic specificity and sensitivity. as a result patients can receive suboptimal and/or inappropriate antimicrobial treatment. in recent years, rapid nucleic acid diagnostics (nad) technolo ... | 2014 | 27920992 |
| glucose metabolism via the entner-doudoroff pathway in campylobacter: a rare trait that enhances survival and promotes biofilm formation in some isolates. | isolates of the zoonotic pathogen campylobacter are generally considered to be unable to metabolize glucose due to lack of key glycolytic enzymes. however, the entner-doudoroff (ed) pathway has been identified in campylobacter jejuni subsp. doylei and a few c. coli isolates. a systematic search for ed pathway genes in a wide range of campylobacter isolates and in the c. jejuni/coli pubmlst database revealed that 1.7% of >6,000 genomes encoded a complete ed pathway, including both c. jejuni and c ... | 2016 | 27920773 |
| advances and challenges in viability detection of foodborne pathogens. | foodborne outbreaks are a serious public health and food safety concern worldwide. there is a great demand for rapid, sensitive, specific, and accurate methods to detect microbial pathogens in foods. conventional methods based on cultivation of pathogens have been the gold standard protocols; however, they take up to a week to complete. molecular assays such as polymerase chain reaction (pcr), sequencing, microarray technologies have been widely used in detection of foodborne pathogens. among mo ... | 2016 | 27920757 |
| identification of a campylobacter coli methyltransferase targeting adenines at gatc sites. | campylobacter coli can infect humans and colonize multiple other animals but its host-associated genes or adaptations are poorly understood. adenine methylation at gatc sites, resulting in mboi resistance of genomic dna, was earlier frequently detected among c. coli from swine but not among turkey-derived isolates. the underlying genetic basis has remained unknown. comparative genome sequence analyses of c. coli 6461, a swine-derived strain with mboi-resistant dna, revealed two chromosomal orfs, ... | 2016 | 27915251 |
| genomic variations leading to alterations in cell morphology of campylobacter spp. | campylobacter jejuni, the most common cause of bacterial diarrhoeal disease, is normally helical. however, it can also adopt straight rod, elongated helical and coccoid forms. studying how helical morphology is generated, and how it switches between its different forms, is an important objective for understanding this pathogen. here, we aimed to determine the genetic factors involved in generating the helical shape of campylobacter. a c. jejuni transposon (tn) mutant library was screened for non ... | 2016 | 27910897 |
| methylome analysis of two xanthomonas spp. using single-molecule real-time sequencing. | single-molecule real-time (smrt) sequencing allows identification of methylated dna bases and methylation patterns/motifs at the genome level. using smrt sequencing, diverse bacterial methylomes including those of helicobacter pylori, lactobacillus spp., and escherichia coli have been determined, and previously unreported dna methylation motifs have been identified. however, the methylomes of xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been ... | 2016 | 27904456 |
| bibliometric analysis of publications on campylobacter: (2000-2015). | campylobacter species are widespread zoonotic pathogens. campylobacter jejuni causes a form of gastroenteritis called campylobacteriosis. campylobacter drug resistance is considered a serious threat. in order to better understand national and international research output on campylobacter, we conducted this bibliometric overview of publications on campylobacter. this study can be used to assess extent of interaction and response of researchers, food regulators, and health policy makers to global ... | 2016 | 27899145 |
| prevalence and antibiotic resistance profile of thermophilic campylobacter spp. of slaughtered cattle and sheep in shiraz, iran | although poultry meat is considered as the main source for human campylobacter infections, there is limited information about non-poultry sources. the present study was aimed to investigate the prevalence and the antibiotic resistance of thermophilic campylobacter spp. in fecal samples of the cattle and sheep in shiraz, iran. a total of 302 fecal samples were obtained from clinically healthy, slaughtered cattle and sheep from shiraz slaughterhouse. the animals were clinically healthy before bein ... | 2016 | 27872721 |
| epidemiology and rapd-pcr typing of thermophilic campylobacters from children under five years and chickens in morogoro municipality, tanzania. | campylobacter species are gram negative and flagellated bacteria under the genus campylobacter, family campylobacteriaceae. these pathogens cause zoonotic infections among human and animal populations. this study was undertaken between december 2006 and may 2007 to determine prevalence, risk factors and genetic diversity of thermophilic campylobacter isolates from children less than 5 years and chickens in morogoro municipality, tanzania. | 2016 | 27871251 |
| isolation of campylobacter spp. from client-owned dogs and cats, and retail raw meat pet food in the manawatu, new zealand. | campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. the disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. the prevalence of campylobacter spp. in dogs and cats varies widely, and data on new zealand pets are limited. this study aimed to investigate the prevalence of campylobacter spp. in dogs, cats and retail raw meat pet food ... | 2016 | 27860343 |
| prevalence of enteropathogens in dogs attending 3 regional dog parks in northern california. | the prevalence and risk factors for infection with enteropathogens in dogs frequenting dog parks have been poorly documented, and infected dogs can pose a potential zoonotic risk for owners. | 2016 | 27859745 |
| antimicrobial resistance of campylobacter species isolated from broilers in live bird markets in shanghai, china. | this study was conducted to determine the prevalence of antimicrobial resistance in campylobacter spp. isolates from broilers in live bird markets (lbms). a total of 209 campylobacter spp. isolates (84 campylobacter jejuni; 125 campylobacter coli) were recovered from 364 broiler cecum samples collected from five lbms in shanghai, china. minimum inhibitory concentrations of 13 antimicrobials were determined using agar dilution method. more than 96% of the campylobacter spp. isolates were resistan ... | 2017 | 27854542 |
| prevalence of campylobacter jejuni and campylobacter coli in raw milk and some dairy products. | this study was accomplished to test raw milk and certain dairy products sold in local markets of qena, egypt, for the presence of campylobacter coli and campylobacter jejuni. | 2016 | 27847427 |
| development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese. | dairy products can harbor various microorganisms (e.g., campylobacter spp., salmonella spp., listeria monocytogenes, verocytotoxin-producing escherichia coli) arising from animal reservoirs, and which can become important sources of foodborne illness. therefore, early detection of food pathogens is crucial to prevent diseases. we wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddpcr) involving eight individual taqman™ reactions to detect ... | 2016 | 27840628 |
| a new lab developed real time pcr assay for direct detection of c. difficle from stool sample without dna extraction. | clostridium difficile is a major cause of nosocomial antibiotic-associated infectious diarrhea and pseudomembranous colitis. detection of c. difficile by anaerobic bacterial culture and/or cytotoxicity assays has been largely replaced by rapid enzyme immunoassays (eia). however, due to the lack of sensitivity of stool eia, we developed a multiplex real-time pcr assay targeting the c. difficile toxin genes tcdb. stool samples from hospitalized pediatric patients suspected of having c. difficile-a ... | 2016 | 27829823 |
| pfge genotyping and molecular characterization of campylobacter spp. isolated from chicken meat. | a total of 70 samples were collected from chicken meat obtained from 10 markets in tehran, iran from which 39 campylobacter coli were isolated. among 10 antibiotics used, maximum resistance was seen to trimethoprim-sulphamethoxazole (sxt) (97.36%), nalidixic acid (94.8%), ciprofloxacin (87.7%), streptomycin (89.72%), and tetracycline (97.4%). no resistance was to gentamycin was observed. none of the campylobacter strains under study harbored integron, suggesting the involvement of other resistan ... | 2016 | 27822247 |
| new weapons to fight old enemies: novel strategies for the (bio)control of bacterial biofilms in the food industry. | biofilms are microbial communities characterized by their adhesion to solid surfaces and the production of a matrix of exopolymeric substances, consisting of polysaccharides, proteins, dna and lipids, which surround the microorganisms lending structural integrity and a unique biochemical profile to the biofilm. biofilm formation enhances the ability of the producer/s to persist in a given environment. pathogenic and spoilage bacterial species capable of forming biofilms are a significant problem ... | 2016 | 27803696 |
| the risk of some veterinary antimicrobial agents on public health associated with antimicrobial resistance and their molecular basis. | the risk of antimicrobial agents used in food-producing animals on public health associated with antimicrobial resistance continues to be a current topic of discussion as related to animal and human public health. in the present review, resistance monitoring data, and risk assessment results of some important antimicrobial agents were cited to elucidate the possible association of antimicrobial use in food animals and antimicrobial resistance in humans. from the selected examples, it was apparen ... | 2016 | 27803693 |
| occurrence of thermotolerant campylobacter in raw poultry meat, environmental and pigeon stools collected in open-air markets. | campylobacteriosis was the most commonly reported zoonosis for confirmed human cases in european union during 2011. poultry meat was very often implicated in campylobacter infections in humans. in italy commerce of raw poultry meat is common in open-air markets: these areas can be considered at high risk of bacterial contamination due to the high presence birds like pigeons. the aim of this study was to collect data about the contamination by thermotolerant campylobacter of raw poultry meat comm ... | 2014 | 27800355 |
| molecular epidemiology, virulence determinants and antimicrobial resistance of campylobacter spreading in retail chicken meat in central china. | campylobacter species are the major food-borne pathogens which could cause bacterial gastroenteritis in humans. contaminated chicken products have been recognized as the primary vehicles of campylobacter transmission to human beings. in this study, the prevalence of campylobacter in retail chicken meat in central china was investigated, and the isolates were further characterized using molecular approaches and tested for antibiotic resistance. | 2016 | 27800028 |
| high rates of contamination of poultry meat products with drug resistant campylobacter in metro manila, philippines. | a total of 265 chicken parts were collected from 15 wet markets and 15 supermarkets in metro manila, philippines. campylobacter spp. was isolated on modified charcoal cefoperazone deoxycholate agar plates and identified through biochemical tests and pcr amplification of genus and species specific genes. antimicrobial resistance profiles were determined following clinical and laboratory standards institute protocols. two hundred seven (78%) campylobacter spp. were isolated. c. jejuni and c. coli ... | 2016 | 27795478 |
| complete genome sequences of multidrug-resistant campylobacter jejuni strain 14980a (turkey feces) and campylobacter coli strain 14983a (housefly from a turkey farm), harboring a novel gentamicin resistance mobile element. | multidrug resistance (mdr) in foodborne pathogens is a major food safety and public health issue. here we describe whole-genome sequences of two mdr strains of campylobacter jejuni and campylobacter coli from turkey feces and a housefly from a turkey farm. both strains harbor a novel chromosomal gentamicin resistance mobile element. | 2016 | 27795285 |
| sensitivity of direct culture, enrichment and pcr for detection of campylobacter jejuni and c. coli in broiler flocks at slaughter. | broiler chicken flocks are a significant source of campylobacter jejuni and campylobacter coli that result in the major public health problem of campylobacteriosis. accurate estimates of the prevalence of both c. coli and c. jejuni in flocks would enhance epidemiological understanding, risk assessment and control options. this study combined results from a panel of 10 detection tests (direct culture, enrichment and pcr) on caecal samples from flocks at slaughter. a parallel interpretation approa ... | 2016 | 27770505 |
| occurrence and genotypes of campylobacter species in broilers during the rearing period. | poultry are the main source of campylobacter infection worldwide. to obtain information on campylobacter-infected flocks and create a reference for preventing and controlling campylobacter at farm level, campylobacter isolates were recovered from broilers and the environments of nine chicken flocks in two farms during growth. the genetic relationship between the campylobacter isolates was determined using multilocus sequence typing. flocks were colonized as early as 3 weeks after introduction to ... | 2017 | 27766896 |
| association of campylobacter jejuni ssp. jejuni chemotaxis receptor genes with multilocus sequence types and source of isolation. | campylobacter jejuni's flagellar locomotion is controlled by eleven chemoreceptors. assessment of the distribution of the relevant chemoreceptor genes in the c. jejuni genomes deposited in the national center for biotechnology information (ncbi) database led to the identification of two previously unknown tlp genes and a tlp5 pseudogene. these two chemoreceptor genes share the same locus in the c. jejuni genome with tlp4 and tlp11, but the gene region encoding the periplasmic ligand binding doma ... | 2016 | 27766165 |
| a direct-sensing galactose chemoreceptor recently evolved in invasive strains of campylobacter jejuni. | a rare chemotaxis receptor, tlp11, has been previously identified in invasive strains of campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that tlp11 specifically interacts with galactose. tlp11 is required for the chemotactic response of c. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. the inactivated mutant displays reduced ... | 2016 | 27762269 |
| use of direct lamp screening of broiler fecal samples for campylobacter jejuni and campylobacter coli in the positive flock identification strategy. | rapid identification of campylobacter-positive flocks before slaughter, following freezing and heat treatment for the campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. we evaluated a loop-mediated isothermal amplification (lamp) assay for the direct screening of naturally contaminated chicken cloacal swabs for c. jejuni/c. coli to compare this assay with conventional quantitative culture methods. in a comparison study o ... | 2016 | 27746777 |
| exploiting bacterial whole-genome sequencing data for evaluation of diagnostic assays: campylobacter species identification as a case study. | the application of whole-genome sequencing (wgs) to problems in clinical microbiology has had a major impact on the field. clinical laboratories are now using wgs for pathogen identification, antimicrobial susceptibility testing, and epidemiological typing. wgs data also represent a valuable resource for the development and evaluation of molecular diagnostic assays, which continue to play an important role in clinical microbiology. to demonstrate this application of wgs, this study used publicly ... | 2016 | 27733632 |
| [multilocus sequence typing analysis of human campylobacter coli in granada (spain)]. | different subtypes of campylobacter spp. have been associated with diarrhoea and a multilocus sequence typing (mlst) method has been performed for subtyping. in the present work, mlst was used to analyse the genetic diversity of eight strains of campylobacter coli. | 2016 | 27701619 |
| persistence and potential viable but non-culturable state of pathogenic bacteria during storage of digestates from agricultural biogas plants. | despite the development of on-farm anaerobic digestion as a process for making profitable use of animal by-products, factors leading to the inactivation of pathogenic bacteria during storage of digestates remain poorly described. here, a microcosm approach was used to evaluate the persistence of three pathogenic bacteria (salmonella enterica derby, campylobacter coli and listeria monocytogenes) in digestates from farms, stored for later land spreading. nine samples, including raw digestates, liq ... | 2016 | 27695451 |
| production of the bioactive compounds violacein and indolmycin is conditional in a maea mutant of pseudoalteromonas luteoviolacea s4054 lacking the malic enzyme. | it has previously been reported that some strains of the marine bacterium pseudoalteromonas luteoviolacea produce the purple bioactive pigment violacein as well as the antibiotic compound indolmycin, hitherto only found in streptomyces. the purpose of the present study was to determine the relative role of each of these two compounds as antibacterial compounds in p. luteoviolacea s4054. using tn10 transposon mutagenesis, a mutant strain that was significantly reduced in violacein production in m ... | 2016 | 27695447 |
| momp from campylobacter jejuni is a trimer of 18-stranded β-barrel monomers with a ca(2+) ion bound at the constriction zone. | the gram-negative organism campylobacter jejuni is the major cause of food poisoning. unlike escherichia coli, which has two major porins, ompc and ompf, c. jejuni has one, termed major outer membrane protein (momp) through which nutrients and antibiotics transit. we report the 2.1-å crystal structure of c. jejuni momp expressed in e. coli and a lower resolution but otherwise identical structure purified directly from c. jejuni. the 2.1-å resolution structure of recombinant momp showed that alth ... | 2016 | 27693650 |
| quantification of growth of campylobacter and extended spectrum β-lactamase producing bacteria sheds light on black box of enrichment procedures. | campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide, and is routinely found in meat originating from poultry, sheep, pigs, and cattle. effective monitoring of campylobacter contamination is dependent on the availability of reliable detection methods. the method of the international organization for standardization for the detection of campylobacter spp. in food (iso 10272-1:2006) recommends the use of bolton broth (bb) as selective enrichment ... | 2016 | 27672384 |
| polyphosphate and associated enzymes as global regulators of stress response and virulence in campylobacter jejuni. | campylobacter jejuni (c. jejuni), a gram-negative microaerophilic bacterium, is a predominant cause of bacterial foodborne gastroenteritis in humans worldwide. despite its importance as a major foodborne pathogen, our understanding of the molecular mechanisms underlying c. jejuni stress survival and pathogenesis is limited. inorganic polyphosphate (poly p) has been shown to play significant roles in bacterial resistance to stress and virulence in many pathogenic bacteria. c. jejuni contains the ... | 2016 | 27672264 |
| functional metagenomics of spacecraft assembly cleanrooms: presence of virulence factors associated with human pathogens. | strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. we wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. three geographically distinct cleanrooms were sampled during the ... | 2016 | 27667984 |
| extraction of total nucleic acids from bacterial isolates using the biomérieux nuclisens easymag total nucleic acid extractor. | the biomerieux nuclisens easymag total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. forty eight isolates were extracted, yielding quantifiable amounts of dna for all isolates. the easymag is appropriate for dna extraction from bacterial isolates and will be incorporated in the clinical laboratory. | 2016 | 27658494 |
| ethyl pyruvate: an anti-microbial agent that selectively targets pathobionts and biofilms. | the microbiota has a strong influence on health and disease in humans. a causative shift favoring pathobionts is strongly linked to diseases. therefore, anti-microbial agents selectively targeting potential pathogens as well as their biofilms are urgently demanded. here we demonstrate the impact of ethyl pyruvate, so far known as ros scavenger and anti-inflammatory agent, on planktonic microbes and biofilms. ethyl pyruvate combats preferably the growth of pathobionts belonging to bacteria and fu ... | 2016 | 27658257 |
| cross-talk between the aeromonas hydrophila type iii secretion system and lateral flagella system. | aeromonas hydrophila is responsible for aeromonad septicaemia in fish, and gastroenteritis and wound infections in humans. the type iii secretion system (t3ss) is utilized by aeromonads to inject protein effectors directly into host cells. one of the major genetic regulators of the t3ss in several bacterial species is the arac-like protein exsa. previous studies have suggested a link between t3ss regulation and lateral flagella expression. the aim of this study was to determine the genetic regul ... | 2016 | 27656180 |
| the outer membrane phospholipase a is essential for membrane integrity and type iii secretion in shigella flexneri. | outer membrane phospholipase a (ompla) is an enzyme located in the outer membrane of gram-negative bacteria. ompla exhibits broad substrate specificity, and some of its substrates are located in the cellular envelope. generally, the enzymatic activity can only be induced by perturbation of the cell envelope integrity through diverse methods. although ompla has been thoroughly studied as a membrane protein in escherichia coli and is constitutively expressed in many other bacterial pathogens, litt ... | 2016 | 27655730 |
| emergence of a potent multidrug efflux pump variant that enhances campylobacter resistance to multiple antibiotics. | bacterial antibiotic efflux pumps are key players in antibiotic resistance. although their role in conferring multidrug resistance is well documented, the emergence of "super" efflux pump variants that enhance bacterial resistance to multiple drugs has not been reported. here, we describe the emergence of a resistance-enhancing variant (named re-cmeabc) of the predominant efflux pump cmeabc in campylobacter, a major zoonotic pathogen whose resistance to antibiotics is considered a serious antibi ... | 2016 | 27651364 |
| complete genome sequences of the plasmid-bearing campylobacter coli strains hc2-48, cf2-75, and co2-160 isolated from retail beef liver. | the complete genome sequences of campylobacter coli strains hc2-48, cf2-75, and co2-160, isolated from retail beef liver, showed the presence of 1,663,782-, 1,711,393-, and 1,683,224-bp circular chromosomes and 44,064-, 44,233-, and 44,228-bp circular plasmids, respectively. this is the first reported campylobacter coli genome sequence isolated from retail beef liver. | 2016 | 27635012 |
| novel methyltransferase recognition motif identified in chania multitudinisentens rb-25(t) gen. nov., sp. nov. | 2016 | 27630623 | |
| non-alcoholic fatty liver and the gut microbiota. | non-alcoholic fatty liver (nafld) is a common, multi-factorial, and poorly understood liver disease whose incidence is globally rising. nafld is generally asymptomatic and associated with other manifestations of the metabolic syndrome. yet, up to 25% of nafld patients develop a progressive inflammatory liver disease termed non-alcoholic steatohepatitis (nash) that may progress towards cirrhosis, hepatocellular carcinoma, and the need for liver transplantation. in recent years, several lines of e ... | 2016 | 27617201 |
| sensitivity to enterocins of thermophilic campylobacter spp. from different poultry species. | campylobacter spp. have been isolated from various animals, including poultry. they are rapidly transmitted throughout broiler sheds by the fecal-oral route. a promising strategy to reduce campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. in this study, inhibition spectrum/activity of different enterocins was evaluated against campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins ... | 2016 | 27602434 |
| point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of campylobacter jejuni. | infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. campylobacter jejuni is an important zoonotic pathogen transmitted to humans mainly via the foodborne route. c. jejuni typically colonizes the gut, but a hypervirulent and rapidly expanding clone of c. jejuni recently emerged, ... | 2016 | 27601641 |
| macrolide resistance mechanisms and virulence factors in erythromycin-resistant campylobacter species isolated from chicken and swine feces and carcasses. | resistance to antimicrobials was measured in 73 isolates of campylobacter jejuni (c. jejuni) and 121 isolates of campylobacter coli (c. coli) from chicken and swine feces and carcasses in korea. both bacterial species showed the highest resistance to (fluoro) quinolones (ciprofloxacin and nalidixic acid) out of the nine antimicrobials tested. erythromycin resistance was much higher in c. coli (19.0%, 23/121) than in c. jejuni (6.8%, 5/73). the mutation in the 23s rrna gene was primarily responsi ... | 2017 | 27593510 |
| antimicrobial resistance of campylobacter isolates from small scale and backyard chicken in kenya. | thermophilic campylobacter species are a major cause of bacterial foodborne diarrhoea in humans worldwide. poultry and their products are the predominant source for human campylobacteriosis. resistance of campylobacter to antibiotics is increasing worldwide, but little is known about the antibiotic resistance in campylobacter isolated from chicken in kenya. in this study, 35 suspected campylobacter strains isolated from faeces and cloacal swabs of chicken were tested for their susceptibility to ... | 2016 | 27570543 |
| campylobacter pouchitis mimicking the appearance of crohn's disease. | an unusual case of campylobacter pouchitis resembling the endoscopic appearance of crohn's disease is reported. | 2016 | 27555972 |
| multiplex pcr assay for identifi cation and differentiation of campylobacter jejuni and campylobacter coli isolates. | campylobacter spp. are important causative agents of gastrointestinal infections in humans. the most frequently isolated strains of this bacterial genus are campylobacter jejuni and campylobacter coli. to date, genetic methods for bacterial identification have not been used in bulgaria. we optimized the multiplex psr assay to identify campylobacter spp. and differentiate c. jejuni from c. coli in clinical isolates. we also compared this method with the routinely used biochemical methods. | 2017 | 27552785 |
| whole-genome sequences of agricultural, host-associated campylobacter coli and campylobacter jejuni strains. | we report here the genome sequences of four agricultural, multidrug-resistant campylobacter spp.: c. coli 11601 and c. jejuni 11601md, isolated from turkey cecum and jejunum, respectively, and c. coli 6067 and c. coli 6461, isolated from turkey-house water and swine feces, respectively. the genomes provide insights on campylobacter antimicrobial resistance and host adaptations. | 2016 | 27540063 |
| multidrug-resistant campylobacter coli in men who have sex with men, quebec, canada, 2015. | 2016 | 27533504 | |
| evaluation of a microfluidic chip system for preparation of bacterial dna from swabs, air, and surface water samples. | the detection of bacterial pathogens from complex sample matrices by pcr requires efficient dna extraction. in this study, a protocol for extraction and purification of dna from swabs, air, and water samples using a microfluidic chip system was established. the optimized protocol includes a combination of thermal, chemical and enzymatic lysis followed by chip-based dna purification using magnetic particles. the procedure was tested using gram-positive bacillus thuringiensis berliner var. kurstak ... | 2016 | 27520284 |