Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. | four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ... | 1993 | 8379970 |
| characterization of a new class of tetracycline-resistance gene tet(s) in listeria monocytogenes bm4210. | the nucleotide sequence of the tetracycline (tc)-minocycline (mc)-resistance determinant of plasmid pip811 from listeria monocytogenes bm4210 has been determined. the gene, designated tet(s), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated m(r) of 72,912. the apparent 68-kda size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcript ... | 1993 | 8370538 |
| [hematologic manifestations in campylobacter coli septicemia. apropos of a case]. | we report the case of a 71-year old male patient with a chronic lymphoid leukaemia of 10 years' duration who abruptly suffered deterioration of this general condition and haematological disturbances suggesting worsening of this blood disease (richter's syndrome). blood cultures demonstrated a campylobacter coli septicaemia. treatment with antibiotics resulted in a return to the previous clinical and biological situation. the various visceral manifestation of the infection are discussed. | 1993 | 8362107 |
| peb1, the major cell-binding factor of campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems. | the protein peb1 (28 kda) is a common antigen and a major cell adherence molecule of campylobacter jejuni and campylobacter coli. we created a bank of chromosomal dna fragments of c. jejuni strain 81-176 using lambda gt11. screening this bank in escherichia coli y1090 cells with antibody raised against purified peb1 enabled us to isolate and to purify a clone with a 2.6-kilobase insert expressing an immunoreactive protein of 28 kda. dna sequencing revealed that the insert contains three complete ... | 1993 | 8360165 |
| direct polymerase chain reaction detection of campylobacter jejuni and campylobacter coli in raw milk and dairy products. | a polymerase chain reaction (pcr) method designed to sensitively detect and identify campylobacter jejuni and campylobacter coli without the need for isolating and culturing strains is described. the intergenic sequence between the flagellin genes flaa and flab was amplified and characterized with a triple primer or seminested primer approach. a total of 50 bacterial strains, 27 of c. jejuni and c. coli and 23 of other species, were tested, giving no false-positive or false-negative results. the ... | 1993 | 8357250 |
| an antigenic polysaccharide from campylobacter coli serotype o:30. structure of a teichoic acid-like antigenic polysaccharide associated with the lipopolysaccharide. | a water-soluble antigenic polysaccharide of high m(r) associated with the lipopolysaccharide has been isolated from phenol-water extraction of cells of campylobacter coli serotype o:30. the polysaccharide and oligosaccharide degradation products formed on o-dephosphorylation and by periodate oxidation followed by reduction have been investigated by one- and two-dimensional 1h, 13c, and 31p nmr. it is concluded that the antigenic polysaccharide has a teichoic acid-like structure with a poly-ribit ... | 1993 | 8349707 |
| the campylobacter sigma 54 flab flagellin promoter is subject to environmental regulation. | the complex flagellum of campylobacter coli vc167 is encoded by two tandemly oriented flagellin genes which are transcribed as two discrete transcriptional units from two different classes of promoters. the flab gene, which encodes the minor flab filament protein, is controlled by a sigma 54 promoter. a transcriptional fusion between a promoterless chloramphenicol acetyltransferase (cat) reporter gene cartridge and c. coli vc167 dna carrying flab transcription and translation signals, including ... | 1993 | 8331072 |
| development of species-specific dna probes for campylobacter jejuni, campylobacter coli, and campylobacter lari by polymerase chain reaction fingerprinting. | the application of polymerase chain reaction (pcr) fingerprinting assays enables discrimination between species and strains of microorganisms. pcr primers aiming at arbitrary sequences in combination with primers directed against the repetitive extragenic palindrome (rep) or enterobacterial repetitive intergenic consensus (eric) motifs generate isolate-specific dna banding patterns. analysis of these pcr fingerprints obtained for 33 isolates of campylobacter jejuni, 30 isolates of campylobacter ... | 1993 | 8314996 |
| efficacy of filter types for detecting campylobacter jejuni and campylobacter coli in environmental water samples by polymerase chain reaction. | a previously developed polymerase chain reaction (pcr) amplification of a target region in the flaa campylobacter flagellin gene was evaluated and adapted for use with environmental water samples. the ability to detect campylobacter jejuni or campylobacter coli in seeded water samples was tested with various filters after concentration and freeze-thaw lysis of the bacterial cells. a nonradioactive probe for the amplified flagellin gene fragment detected as little as 1 to 10 fg of genomic dna and ... | 1993 | 8285708 |
| interaction of helicobacter pylori (strain 151) and campylobacter coli with human peripheral polymorphonuclear granulocytes. | helicobacter pylori associated gastritis is characterized by dense mucosal inflammatory infiltrations with predominantly neutrophilic granulocytes, together with a local and systemic immune response. nevertheless, the natural course of the infection is chronic in nature, and active phagocytosis of h. pylori by mucosal granulocytes was only rarely observed. the aim of the present study was to investigate with electronmicroscopic methods the interaction of h. pylori with freshly harvested human pe ... | 1993 | 8280958 |
| [unusual infectious complication in a pregnant woman. spontaneous abortion caused by campylobacter coli]. | 1993 | 8259031 | |
| transformation of helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. | most strains of helicobacter pylori are naturally competent for uptake of chromosomal dna. transformation frequencies for streptomycin resistance or rifampicin resistance markers ranged from 1 x 10(-4) to 1 x 10(-3) per viable cell using a plate transformation procedure. transformation of a metronidazole resistance marker (mtrr) was demonstrated when either a laboratory-derived mutant or a mtrr clinical isolate were used as the source of donor dna. mtrr was transformed at a frequency of 3 x 10(- ... | 1993 | 8254319 |
| use of nonradioactive dna probes to identify a campylobacter jejuni strain causing abortion. | a case of human abortion due to a campylobacter infection is reported. cultures revealed two morphologically different isolates with large and small colonies respectively. using conventional methods of identification, the large colonies were identified as campylobacter jejuni and the small colonies as campylobacter coli. dot blot hybridization and determination of rdna restriction fragment patterns revealed that both colony types were the same strain of campylobacter jejuni. this observation ill ... | 1993 | 8223663 |
| iron-regulated proteins in outer membranes of campylobacter jejuni diarrhoea isolates and immune response to the proteins in patients. | the outer membrane protein (omp) profiles from 8 campylobacter jejuni and 5 campylobacter coli fecal isolates grown under various conditions were compared by sds-page. the bacteria were grown under usual conditions, in iron-deficient medium (dip) and on iron-supplemented medium (fe). the omp profiles of most bacterial strains grown under usual conditions, or in the fe-supplemented medium, contained four major bands of approximately 31, 45, 63-66 and 97 kda, and in addition, a number of minor ban ... | 1994 | 8167428 |
| bacteraemia caused by campylobacter spp. | the genus campylobacter has become increasingly recognised as the cause of various infections. campylobacter jejuni and c coli cause acute gastroenteritis in man all over the world. c jejuni enteritis can lead to bacteraemia, but its actual incidence remains unknown. seven cases of bacteraemia caused by c jejuni or c coli are reported, from the blood of seven patients: five immune deficient adults; a newborn baby; and a patient who had had abdominal surgery. patients who develop diarrhoea as a r ... | 1994 | 8132835 |
| azithromycin resistance in campylobacter jejuni and campylobacter coli. | the mics of erythromycin, azithromycin and ciprofloxacin were determined for 60 human fecal isolates of campylobacter. of these, 30 strains selected on the basis of their resistance to erythromycin by disk diffusion were highly resistant to both erythromycin and azithromycin. nine of these selected isolates were resistant to ciprofloxacin. the remaining 30 strains were non-selected, consecutive isolates of campylobacter susceptible to erythromycin by disk diffusion and were shown to be two- to f ... | 1993 | 8112360 |
| distribution and polymorphism of the flagellin genes from isolates of campylobacter coli and campylobacter jejuni. | the complex flagellar filaments of the lio8 serogroup member campylobacter coli vc167 are composed of two highly related subunit proteins encoded by the flaa and flab genes which share 92% identity. using oligonucleotide primers based on the known dna sequence of both the flaa and flab genes from c. coli vc167 in the polymerase chain reaction, we have shown conservation of both fla genes among isolates within the lio8 heat-labile serogroup by digestion of the amplified product with psti and ecor ... | 1993 | 8098328 |
| macro-fingerprinting analysis at the chromosomal genomic dna level of isolates of thermophilic campylobacter coli and c. jejuni, by pulsed-field gel electrophoresis. | chromosomal genomic dna from 25 strains of thermophilic campylobacter coli and c. jejuni were analysed after pulsed-field gel electrophoresis (pfge) of restriction fragments generated by sali and smai to evaluate the utility of macro-fingerprinting analysis and to assess the possible subtyping of the isolates. two rare-cutting restriction enzymes, sali and smai, allowed the subclassification of eight strains of c. coli into six types. sali facilitated the subclassification of seventeen strains o ... | 1994 | 8070288 |
| characterization of the sat4 gene encoding a streptothricin acetyltransferase in campylobacter coli be/g4. | the sat4 streptothricin resistance gene from campylobacter coli be/g4 was cloned into puc18, and its nucleotide sequence was determined. streptothricin acetyltransferase activity was detected in escherichia coli cells containing recombinant plasmid pat132 which carries the sat4 gene as an insert. the deduced amino acid sequence displayed 21-27% amino acid identity with streptothricin acetyltransferases from e. coli and streptothricin producers streptomyces lavendulae and streptomyces noursei. th ... | 1994 | 8056285 |
| phylogenetic and molecular characterization of a 23s rrna gene positions the genus campylobacter in the epsilon subdivision of the proteobacteria and shows that the presence of transcribed spacers is common in campylobacter spp. | the nucleotide sequence of a 23s rrna gene of campylobacter coli vc167 was determined. the primary sequence of the c. coli 23s rrna was deduced, and a secondary-structure model was constructed. comparison with escherichia coli 23s rrna showed a major difference in the c. coli rrna at approximately position 1170 (e. coli numbering) in the form of an extra sequence block approximately 147 bp long. pcr analysis of 31 other strains of c. coli and c. jejuni showed that 69% carried a transcribed space ... | 1994 | 8045890 |
| cloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from campylobacter jejuni and campylobacter coli. | genes encoding superoxide dismutase (sod: ec 1.15.1.1) were cloned from campylobacter jejuni nctc 11351 and campylobacter coli ua585 by heterologous complementation of a sod-deficient escherichia coli mutant. deletion analysis of the cloned c. jejuni dna assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. the deduced gene product of 220 amino acids was 71% identical to the e. coli iron-containing sod and 60% identical to the e. coli manganese-containing s ... | 1994 | 8025686 |
| detection of flagellar antigen of campylobacter jejuni and campylobacter coli in canine faeces with an enzyme-linked immunosorbent assay (elisa)--new prospects for diagnosis. | a new diagnostic procedure was developed to detect the flagellar antigen of campylobacter jejuni and campylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluated using species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. the assay detected all c. jejuni or c. coli infected specimens c ... | 1994 | 7975201 |
| seroreactivity to campylobacter jejuni and gangliosides in patients with guillain-barré syndrome. | seroreactivities to campylobacter jejuni or campylobacter coli and to the gangliosides gm1 and gd1b were studied by quantitative and immunoglobulin class-specific elisas in 42 patients with recent onset of acute guillain-barré syndrome (gbs), in 39 patients with positive campylobacter serology but no neurologic disease, and in 52 healthy blood donors. gbs patients showed positive reactivities to c. jejuni in 57%, 26%, and 2% for igg, iga, and igm, respectively, while blood donors had correspondi ... | 1994 | 7930724 |
| biotyping of campylobacter jejuni and campylobacter coli infections in spain. | biochemical profiles were studied in 102 campylobacter isolates from patients affected by enteric disease in orense (spain) over a 1-year period. the isolates were identified as hippurate-positive campylobacter jejuni (n = 90), campylobacter coli (n = 10) and hippurate-negative c. jejuni (n = 2). seventy-seven of the hippurate-positive and both hippurate-negative c. jejuni isolates were biotyped as c. jejuni subsp. jejuni biotype 1, nine as c. jejuni subsp. jejuni biotype 2 and four as c. jejuni ... | 1994 | 7884224 |
| campylobacter hyoilei sp. nov., associated with porcine proliferative enteritis. | campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain rmit 32at (t = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. the phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other campylobacter spp. commonly isolated from pigs. the results of probing of clai-, ecorv-, or bglii-cleaved genomic dnas from c. hyoilei stra ... | 1995 | 7857809 |
| cryptolepis sanguinolenta activity against diarrhoeal bacteria. | cryptolepine is the main alkaloid of cryptolepis sanguinolenta (lindl.) schlechter, a plant used in traditional medicine in west africa. the minimal inhibitory concentrations (mics) of cryptolepine, ethanol and aqueous extracts of cryptolepis sanguinolenta root were determined for 65 strains of campylobacter jejuni, 41 strains of campylobacter coli isolated from sporadic cases of gastroenteritis in portugal and 86 strains of vibrio cholerae isolated from patients with enteric infections in angol ... | 1994 | 7853867 |
| [an enzyme-linked immunosorbent assay for the detection of igg antibodies against urease of helicobacter pylori]. | we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of igg antibodies against helicobacter pylori (hp) using purified hp urease as an antigen. the urease was purified from ultrasonicated extract of hp by nacl linear gradient system on deae-sepharose 4b chromatography. two molecular weight bands, 65kd and 27kd were observed on a sds-page gel in the purified urease sample. the urease antigen did not crossreact to rabbit antibodies prepared against campylobacter coli an ... | 1994 | 7844892 |
| prenatal diagnosis of microbial invasion of the amniotic cavity with campylobacter coli in preterm labour. | 1995 | 7833319 | |
| identification of campylobacter jejuni, campylobacter coli and campylobacter lari by the nucleic acid amplification system nasbar. | nasbar, an isothermal amplification technique for nucleic acids, was evaluated for the specific identification of campylobacter jejuni, camp. coli and camp. lari. a set of primers and a probe were chosen from the 16s rrna sequence of campylobacter. the probe was hybridized in solution with the amplified nucleic acids of 12 campylobacter species and nine other gram-negative bacteria. the probe was shown to hybridize specifically to the amplified single-stranded rna of camp. jejuni, camp. coli and ... | 1994 | 7822229 |
| urinary tract infection caused by quinolone-resistant campylobacter coli. | 1994 | 7813508 | |
| identification of campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein. | to facilitate discrimination between the closely related enteropathogens campylobacter jejuni and c. coli, unique differences in antigenic surface structure were examined. a genomic library of c. jejuni 81116 was constructed in plasmid pbluescriptiisk- and expressed in escherichia coli k-12. rabbit hyperimmune serum raised against c. jejuni atcc 29428 recognized a clone expressing a c. jejuni 24-kda membrane-associated protein. antiserum raised against sonicated recombinant e. coli expressing th ... | 1995 | 7790451 |
| restriction fragment length polymorphisms among the flagellar genes of the lior heat-labile serogroup reference strains and field strains of campylobacter jejuni and c. coli. | several typing systems have been described for campylobacter jejuni and c. coli, to assess the complex epidemiology of these important enteric pathogens. in the present study two typing methods, slide agglutination according to the lior scheme, and the demonstration of restriction-fragment length polymorphisms (rflp) of flagellar genes, have been used in parallel on a set of 194 strains. this set comprised 118 sero-reference strains of c. jejuni and c. coli of the lior scheme, as well as 76 clin ... | 1995 | 7781730 |
| investigation of helicobacter pylori ascorbic acid oxidating activity. | helicobacter pylori sonicate was shown to oxidize ascorbic acid. ascorbic acid oxidation was determined by chromatography combined with electrochemical detection. water soluble ascorbic acid oxidase activity was rather independent of ph with a ph optimum around 2. by gel filtration the oxidizing activity co-eluted with an absorbency peak at 408 nm. the relative molecular mass (mr) was approximately 14,000. it is suggested that this oxidating activity was caused by a cytochrome c-like molecule. a ... | 1995 | 7773246 |
| incidence and transmission of antibiotic resistance in campylobacter jejuni and campylobacter coli. | one-hundred and two campylobacter clinical isolates were characterized for their in-vitro resistance to erythromycin (1.9%), three fluoroquinolones (31.3-34.3%), tetracycline (43.1%), kanamycin (4.8%), ampicillin (18.6%) and other 16 antimicrobial agents. conjugative transfer of tetracycline and kanamycin resistances among these strains was achieved and small plasmids of 4.3, 4 and 1.9 kb were observed in kanamycin-resistant campylobacter coli strains. | 1995 | 7768766 |
| a pcr assay for the detection of campylobacter jejuni and campylobacter coli in water. | a pcr assay has been developed for the detection of campylobacter jejuni and camp. coli in water samples. the sample is filtered through a membrane which is subjected to sonication to release the impacted cells. after removal of the filter from the cell suspension and a freeze/thaw cell lysis step, a semi-nested pcr is carried out on the filtrate using the primers cf02, cf03 and cf04 (camp. jejuni flaa and flab gene sequences). incorporation of a sonication stage allows removal of the filter mem ... | 1994 | 7765441 |
| expression and characterization of campylobacter jejuni benzoylglycine amidohydrolase (hippuricase) gene in escherichia coli. | the basis for the difference between campylobacter jejuni and campylobacter coli is the presence and expression of the n-benzoylglycine amidohydrolase (hippuricase) gene only in c. jejuni. a pbr322 recombinant clone (phip-o) of c. jejuni tgh9011 capable of converting hippuric acid into benzoic acid and glycine, the hallmark of hippuricase activity, was characterized and sequenced. the hippuricase gene (hipo) was identified by use of deletion subclones and insertional inactivation. the transcript ... | 1995 | 7730270 |
| [diarrheal diseases in 1 to 4 week old suckling piglets from problem herds: microbial spectrum, histology, enzyme histochemistry]. | postmortem investigation was performed on 54 scouring (soon after onset of diarrhea) and 23 healthy suckling piglets. the animals were 1 to 4 weeks of age and were collected from 27 herds. in 21 herds diarrhea had been observed for a long time in piglets in this age group. coccidia were found in 34 piglets, rotavirus in 2, cryptosporidia in 2, enterotoxigenic e. coli in 6, attaching and effacing e. coli in 1, cl. perfringens type c in 1, campylobacter coli in 53 and c. hyointestinalis in 8 pigle ... | 1994 | 7709191 |
| occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples. | twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ... | 1994 | 7703010 |
| cross-reactive antigens shared by pseudomonas aeruginosa, helicobacter pylori, campylobacter jejuni, and haemophilus influenzae may cause false-positive titers of antibody to h. pylori. | cystic fibrosis (cf) patients suffer from many of the gastrointestinal conditions which occur in non-cf individuals, e.g., dyspepsia and peptic ulceration. these symptoms may be caused by helicobacter pylori but could also be due to either pancreatic insufficiency or the intensive antibiotic treatment used in cf patients. since cf patients chronically infected with pseudomonas aeruginosa produce antibodies against a wide range of antigens, including antigens common to many other bacteria, e.g., ... | 1995 | 7697522 |
| common somatic o and heat-labile serotypes among campylobacter strains from sporadic infections in the united states. | somatic o (formerly heat-stable) and heat-labile (hl) serotyping methods are commonly used to type campylobacter jejuni and campylobacter coli isolates. although both systems are effective, the labor and time required for each have limited their application. these systems can be simplified by reducing the number of antisera used. to find an appropriate panel of antisera, we determined the distribution of common serotypes in the united states among a representative sample of 298 campylobacter iso ... | 1993 | 7686183 |
| molecular characterization of kata from campylobacter jejuni and generation of a catalase-deficient mutant of campylobacter coli by interspecific allelic exchange. | a gene encoding catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; ec 1.11.1.6) from campylobacter jejuni was cloned by functional complementation of a catalase-deficient mutant of escherichia coli. the catalase structural gene, designated kata, was assigned by subcloning and its nucleotide sequence determined. the deduced protein product of 508 amino acids, which had a calculated molecular mass of 58,346 da, was found to be structurally and enzymically similar to hydrogen-peroxidases ... | 1995 | 7670638 |
| a survey of campylobacter bacteremia in three danish counties, 1989 to 1994. | over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ... | 1995 | 7660078 |
| anti-gm1 igg antibodies and campylobacter bacteria in guillain-barré syndrome: evidence of molecular mimicry. | in guillain-barré syndrome antibodies to gm1 and the presence of an antecedent campylobacter jejuni infection are correlated with a more severe course of the disease. from a group of 137 consecutive gbs patients, 11 sera had elevated titers of anti-gm1 igg antibodies during the acute stage of disease. each serum sample was preincubated with three different penner serotypes of whole c. jejuni (pen o:4/59, pen o:41) and campylobacter coli (pen o:22) bacteria. the pen o:4/59 serotype, isolated from ... | 1995 | 7654064 |
| rapid detection of campylobacter jejuni and campylobacter coli isolated from clinical specimens using the polymerase chain reaction. | seventeen campylobacter strains isolated from 16 children hospitalised with acute diarrhea were analysed by in vitro enzymatic amplification using two sets of oligonucleotide primers specific for campylobacter jejuni and campylobacter coli, respectively. thirteen strains (76%) were identified as campylobacter jejuni and four strains (24%) as campylobacter coli. subsequent bacteriological identification confirmed the identity of the same 13 campylobacter jejuni strains and the 4 campylobacter col ... | 1995 | 7649203 |
| [infectivity of campylobacter jejuni and campylobacter coli in chickens]. | the infectivity of four campylobacter (c.) jejuni strains and one c. coli strain was evaluated by the reisolation rate from cloacal swabs after oral infection of chickens at four weeks of age. one strain did not colonize the digestive tract, another infected only a part of the experimental group and a third was excreted at a lower percentage in the first two weeks p.i. compared to the other two strains. in correlation to age, the excretion rate was highest in 7-week-old birds. in groups of 4 and ... | 1995 | 7646423 |
| differentiation of campylobacter jejuni and campylobacter coli strains by using restriction endonuclease dna profiles and dna fragment polymorphisms. | the chromosomal dna fragment patterns from a total of 169 campylobacter jejuni and campylobacter coli isolates from poultry and humans were analyzed by using dna restriction endonucleases clai and ecorv. the dna restriction patterns produced by clai and ecorv consisted of unique dna fragments of 9 to 9.5 kb and 3.5 kb generated with clai and a single unique fragment of 3.0 kb produced by ecorv. these patterns were obtained with all strains of c. jejuni tested. the dna restriction patterns were f ... | 1995 | 7615717 |
| targeted and random mutagenesis of the campylobacter coli chromosome with integrational plasmid vectors. | a number of integrational vectors were developed for use as genetic tools in the food-borne pathogen campylobacter coli. integration of the plasmids occurred following genetic recombination via a campbell-like mechanism. for an integrative plasmid containing a dna fragment internal to the c. coli catalase gene, the insertion was mutagenic and led to a catalase-deficient phenotype. a procedure for generating random mutations in the c. coli chromosome, with these suicide-plasmids, was developed. i ... | 1995 | 7606191 |
| comparison of two selective media and a membrane filter technique for isolation of campylobacter species from diarrhoeal stools. | diarrhoeal stool specimens from 415 patients were examined for campylobacter spp. by culture on charcoal cefoperazone deoxycholate agar (ccda), skirrow medium and columbia blood agar overlaid with a 0.65 micron pore size membrane filter. forty-eight campylobacter strains were isolated from 45 (10.8%) specimens by all media; 44 were campylobacter jejuni (91.7%), three were campylobacter coli (6.3%) and one was campylobacter hyointestinalis (2.0%). the percentages of campylobacter-positive specime ... | 1995 | 7588831 |
| effect of the complement system on the sensitivity of campylobacter jejuni and campylobacter coli to human blood serum. | we determined the sensitivity of five strains of campylobacter jejuni and c. coli isolated from children with diarrhea and from chicken feces to normal human blood serum (undiluted and at concentrations of 10, 30, 50 and 70%), a hypogammaglobulinemic serum and a complement-deficient serum. both species were highly sensitive to the bactericidal activity of human serum, regardless of their source. the highest bactericidal activity was observed with pooled fresh normal serum, with bacterial surviva ... | 1995 | 7581046 |
| high-resolution genotyping of campylobacter coli identifies clones of epidemiologic and evolutionary significance. | campylobacter coli strains from clinical and other sources were examined in terms of o (heat-stabile; hs) serotype and by several molecular typing techniques. restriction fragment length polymorphism (rflp) around the three 16s rrna genes revealed 10 variants, none found in campylobacter jejuni. rflp analysis of a polymerase chain reaction amplicon generated from the flagellin gene (flaa) yielded 11 polymorphism groups, some of them linked to hs serotypes. enlarged flaa genes, contributing three ... | 1995 | 7561196 |
| detection and identification of campylobacter coli and campylobacter jejuni by two-step polymerase chain reaction. | flagellin gene was used as target sequence to detect and distinguish c. coli and c. jejuni by a "nested pcr" technique. the method shows a high level of sensitivity and specificity. application of this rapid diagnostic tool could provide further information about epidemiological and pathogenetic implications of each of these two microorganisms. | 1995 | 7552696 |
| structural and antigenic characteristics of campylobacter coli flaa flagellin. | the polar flagellar filament of campylobacter coli vc167 is composed of two highly related (98%) flagellin subunit proteins, flaa and flab, whose antigenic specificities result from posttranslational modification. flaa is the predominant flagellin species, and mutants expressing only flaa form a full-length flagellar filament. although the deduced m(r) of type 2 (t2) flaa is 58,884 and the apparent m(r) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is 59,500, the solution weight-a ... | 1994 | 7515043 |
| discrimination among thermophilic campylobacter species by polymerase chain reaction amplification of 23s rrna gene fragments. | by comparing nucleic acid sequences determined for one of the most variable areas of 23s rrna genes of 23 campylobacter strains, we were able to identify regions specific for thermophilic campylobacter strains. oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. one primer pair selectively detected all thermophilic campylobacter species, while four other primer pairs allowed discrimination among the thermophilic species campyl ... | 1993 | 7508460 |
| genetic organization of a small cryptic plasmid of helicobacter pylori. | a 2.9-kb cryptic plasmid of helicobacter pylori (hp), phel1, was isolated and the complete nucleotide (nt) sequence was determined. an open reading frame (orf1) was identified encoding a putative polypeptide of 63,709 da, the existence and correct size of which was confirmed by t7 promoter expression analysis. the orf1 sequence showed strong amino-acid sequence identity to a recently identified putative orf1 protein of a cryptic hp plasmid, phpm180, and significant homologies to putative rep pro ... | 1995 | 7489910 |
| production of monoclonal antibody specific to campylobacter jejuni and its potential in diagnosis of campylobacter enteritis. | a monoclonal antibody (mab 3g6) specific for campylobacter jejuni and campylobacter coli was produced by immunizing balb/c mice with a local strain of c.jejuni (28.1). no cross-reactivity was observed with enterobacteriaceae controls. by immunoprecipitation, mab 3g6 identified a major protein band of molecular weight 45 kda and also gave a slight reactivity with 30 and 55 kda proteins. using an indirect enzyme-linked immunosorbent assay, mab 3g6 was able to detect c.jejuni suspended in stool wit ... | 1995 | 7488346 |
| [the occurrence of campylobacter fetus subsp. jejuni and salmonella bacteria in some wild birds (author's transl)]. | an investigation was carried out into the occurrence of campylobacter fetus subsp. jejuni and salmonella species in some wild birds. a total of 129 birds was examined, consisting of 71 pigeons, 54 seagulls, three crows and one raven. campylobacter bacteria were isolated from 32 birds (24.8%), of which three were pigeons, 27 seagulls and two were crows. of the 27 campylobacter strains isolated from seagulls, four had the biochemical characteristics of the nartc biotype described by skirrow and be ... | 1981 | 7335487 |
| experimental infection of pigs with oesophagostomum dentatum: pathogenesis and parasitology of repeated mass infection. | pigs receiving a limited ration of 1 kg commercial feed per day were infected daily with 50,000 oesophagostomum dentatum larvae. the animals exhibited serious diarrhoea and anorexia. although there was neither anaemia nor hypoproteinaemia, there was a significant decrease in plasma sodium and an increase in blood urea nitrogen at the end of the experiment. large numbers of third and fourth stage larvae were found in the ileal, caecal and colonic mucosae. only fourth stage larvae, never adults, w ... | 1981 | 7313309 |
| aetiology of acute diarrhoea in adults. | we have studied 73 adults with acute diarrhoea and identified a micro-organism or toxin likely to be the cause in 58%. in addition to routinely cultured bacteria, campylobacter coli/jejuni and clostridium difficile were important pathogens in the community. patients who developed diarrhoea after antibiotic use had a distinctive clinical syndrome and comprised the third largest group of cases. clinical, epidemiological, and histological features in an additional group with negative cultures and n ... | 1981 | 7250751 |
| [isolation of campylobacter coli/jejuni from samples of human feces in the province of pisa]. | 1982 | 7171243 | |
| outer membrane characteristics of campylobacter jejuni. | outer membranes were isolated from type strains and wild-type isolates of campylobacter jejuni and campylobacter coli by sodium lauryl sarcosinate extraction, and the polypeptide complement and lipopolysaccharide (lps) content were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the protein profiles exhibited by membranes from both species were quite similar, but could be distinguished from the type strain of the genus, c. fetus subsp. fetus cip5396. the sodium dodecyl sul ... | 1982 | 7152677 |
| pathogenicity of campylobacter jejuni isolates from animals and humans. | fourteen isolates of campylobacter jejuni of different serotypes and one campylobacter coli isolate, from various human and animal sources, were tested for potential pathogenic mechanisms. enterotoxin production was not detected in the infant mouse test or by calf and piglet ligated intestinal loop studies. isolates were not invasive by the sereny test. all isolates associated with and penetrated hela cells, although both actions occurred generally in a minor way under the conditions of our stud ... | 1982 | 7141696 |
| production of enteritis in pigs by the oral inoculation of pure cultures of campylobacter coli. | pure cultures of an isolate of campylobacter coli obtained from small intestinal lesions in a seven-day-old piglet were used to inoculate hysterectomy derived, colostrum deprived piglets, conventional sucking piglets and conventional weaned pigs in three separate controlled experiments. rectal temperatures rose to 40 degrees c in inoculated hysterectomy derived, colostrum deprived pigs within four days of infection and a mucoid yellowish diarrhoea containing occasional flecks of blood developed. ... | 1982 | 7135771 |
| recovery of campylobacter jejuni and campylobacter coli from inoculated foods by selective enrichment. | a direct enrichment procedure was developed to selectively recover small numbers of campylobacter jejuni, c. coli, and nalidixic acid-resistant thermophilic campylobacter from foods. the procedure includes an enrichment medium composed of brucella broth, 7% lysed horse blood, 0.3% sodium succinate, 0.01% cysteine hydrochloride, vancomycin (15 micrograms/ml), trimethoprim (5 micrograms/ml), polymyxin b (20 iu/ml), and cycloheximide (50 micrograms/ml) that is inoculated with 10 or 25 g of food and ... | 1982 | 7103488 |
| [hepatitis caused by campylobacter coli (author's transl)]. | campylobacter coli is known to cause ulcerous enterocolitis, but hepatitis has not yet been reported. a 50-year-old woman without history of liver disease was admitted with diarrhoea, fever, poor general condition, subicterus and enlarged liver. campylobacter coli was grown in haemoculture, and a specific antibiotic treatment resulted in complete cure. the results of haemoculture, the necrosis and polymorphonuclear infiltrates found in liver biopsies, the return to normal of biochemical tests an ... | 1982 | 7070993 |
| experimental infection of the bovine udder with campylobacter coli/jejuni. | five quarters of the udders of two lactating cows were infected by intramammary inoculation with campylobacter coli/jejuni in doses ranging from 2.6 colony-forming units (c.f.u.) to 3.8 x 10(9) c.f.u. the infected quarters developed clinical mastitis and the campylobacters were reisolated in large numbers from the milk. the milk from the uninfected quarters, and blood and faeces remained free of the organisms. the campylobacters could only be isolated by incubation of culture plates in a microae ... | 1980 | 7052224 |
| the survival of campylobacter coli/jejuni in unpasteurised milk. | 1981 | 6897896 | |
| occurrence of plasmid dna in serologically defined strains of campylobacter jejuni and campylobacter coli. | forty campylobacter jejuni and 17 campylobacter coli strains that constitute the set of reference strains for our serotyping scheme were each examined for the presence of plasmid dna. agarose gel electrophoresis of alkaline-extracted dna showed the occurrence of 29 bands in 11 c. jejuni strains and 40 bands in c. coli strains. plasmids ranged in size from 1.6 to 70 megadaltons. most strains that carried plasmids had between 2 and 6 of them; however, one strain had 14 plasmids, and two strains co ... | 1983 | 6840847 |
| serotypes of campylobacter jejuni and campylobacter coli in bacteremic, hospitalized children. | 1983 | 6833802 | |
| house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni. | a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ... | 1983 | 6830213 |
| the serotype and biotype distribution of clinical isolates of campylobacter jejuni and campylobacter coli over a three-year period. | two hundred eighty-five isolates of campylobacter jejuni-campylobacter coli from children with gastroenteritis at the hospital for sick children (toronto, canada) over a three-year period were biotyped by the hippurate hydrolysis test and serotyped on the basis of thermostable, soluble antigens by the passive hemagglutination technique. hippurate-negative strains (c. coli) were only 3.2% of the isolates. ninety-seven percent of the isolates were serotypable with 55 antisera. about half of the st ... | 1983 | 6827141 |
| dna relatedness among strains of campylobacter jejuni and campylobacter coli with divergent serogroup and hippurate reactions. | eleven strains of campylobacter from earlier fluorescent-antibody studies were examined by dna hybridization to determine their species. three of the strains hydrolyzed sodium hippurate, and eight did not. four of the hippurate-negative strains were in campylobacter jejuni serogroups, and the remaining strains were in both c. jejuni and campylobacter coli serogroups. dna relatedness to type strains of c. jejuni and c. coli indicated that all three of the hippurate-positive strains and two of the ... | 1984 | 6746886 |
| campylobacter coli septicaemia associated with septic abortion. | a 19-year-old patient was 27 weeks pregnant when admitted to hospital with fever, chills and premature labours. the following day she aborted. campylobacter coli was isolated from blood cultures, maternal placenta and amniotic fluid and from the ear, nose and pharynx of the stillbirth. campylobacter could not be isolated from stool specimens after antimicrobial treatment had been started. | 1984 | 6735483 |
| serotyping and biotyping of campylobacter jejuni and campylobacter coli from sporadic cases and outbreaks in norway. | of 172 thermophilic campylobacters isolated from human cases of gastroenteritis in norway, 149 (86.6%) were classified as campylobacter jejuni, whereas 23 isolates (13.4%) belonged to campylobacter coli. c. jejuni biotype 1 comprised 66.3% and c. jejuni biotype 2 comprised 20.3% of the total number. using 50 unabsorbed antisera, we were able to serotype 109 (80.1%) of 136 campylobacters on the basis of heat-stable antigens identified by means of passive hemagglutination. the typable strains fell ... | 1984 | 6699144 |
| a comparison of procedures for the isolation of campylobacters from seagull faeces. | two enrichment broths (preston and roman & doyle's) and four solid media (preston, skirrow's, butzler's and blaser's) were compared to determine their relative efficiencies in recovering campylobacters from 389 freshly voided seagull faeces, 276 of which were found to contain campylobacters by one or more of the procedures used. a combination of enrichment in preston medium followed by plating on to preston agar gave the highest number of isolates (263). enrichment in fluid media was shown to be ... | 1983 | 6663060 |
| pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model. | pathogenicity of 17 campylobacter isolates for pregnant guinea pigs was investigated. of 14 isolates, 12 (86%) produced rates of abortion ranging from 13% to 87%. two isolates did not produce abortion. reference strains of c fetus subsp venerealis produced abortion in 60% to 87% and c fetus subsp fetus produced abortion in 60% of the guinea pigs. inoculated organisms were recovered from uterus, blood, liver, kidney, spleen, and gallbladder of the guinea pigs at rates as high as 83% for 2 ovine i ... | 1983 | 6650962 |
| erythromycin resistance only found in campylobacter coli. | 1983 | 6643337 | |
| isolation and characterization of campylobacter jejuni and campylobacter coli from domestic and wild mammals in norway. | a total of 1,262 domestic and wild mammals from norway were surveyed for fecal carriage of campylobacter jejuni and campylobacter coli. of the five species of domestic mammals examined, the highest isolation rate was recorded among swine (100.0%), followed by sheep (8.1%) and cows (0.8%). no strains were recovered from horses or goats. among wild mammals, c. jejuni was isolated from 1 of 23 hares, and no isolated were obtained from three species of cervids and three species of rodents. of the 13 ... | 1983 | 6639033 |
| swine dysentery: protection against experimental challenge following single dose parenteral immunisation with inactivated treponema hyodysenteriae. | groups of five pigs were vaccinated at three to four weeks old with either formolised treponema hyodysenteriae in oil adjuvant alone, formolised t hyodysenteriae in oil adjuvant plus formolised campylobacter coli in oil adjuvant, or sterile medium in oil adjuvant (as a control). each group was challenged four weeks after vaccination by oral dosing on two consecutive occasions with pure cultures of the homologous strain of t hyodysenteriae plus direct contact with two pigs exhibiting severe swine ... | 1983 | 6635345 |
| campylobacter jejuni and campylobacter coli serotypes isolated from chickens, cattle, and pigs. | a total of 191 campylobacter jejuni and 125 campylobacter coli were isolated from the intestinal content of 398 chickens, 421 cattle, and 203 pigs. all 108 chicken isolates and 73 of 80 cattle isolates were c. jejuni, but 115 of the 118 pig isolates were c. coli. a total of 84% of the c. jejuni and 64% of the c. coli isolates were typed on the basis of thermostable antigens with 20 antisera prepared against frequently occurring serotypes in campylobacter enteritis in man (15 c. jejuni, 6 c. coli ... | 1983 | 6630466 |
| serotyping of campylobacter jejuni and campylobacter coli on the basis of thermostable antigens. | hippurate hydrolysis tests performed on the serotype reference strains of the serotyping scheme based on thermostable antigens under development for campylobacter jejuni showed that 42 strains were campylobacter jejuni and 17 were campylobacter coli. moreover, only four (0.2%) of 2025 hippurate positive campylobacter jejuni isolates reacted in campylobacter coli antisera and 12 (4.3%) of the 282 campylobacter coli reacted in campylobacter jejuni antisera. evidently each species has its own array ... | 1983 | 6628376 |
| identification and characterization of campylobacter jejuni outer membrane proteins. | outer membrane proteins from isolates of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125i-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. most isolates contained a single major band with ... | 1983 | 6618667 |
| detection of campylobacter coli in diarrhoeal and non-diarrhoeal children in india. | the role of campylobacter coli as an etiological agent of diarrhea in children and the pathogen's presence in nondiarrheal children was examined in the region of chandigarh, india, from may, 1981, to july, 1982. a total of 768 fecal samples from 447 children, 159 symptomatic and 288 asymptomatic, was collected, along with food, water, soil, and animal dung samples from the village. in diarrheal and nondiarrheal groups, c. coli was isolated with frequencies of 3.1% and 3.8% respectively. this ... | 1984 | 6530531 |
| chemical studies of partially hydrolysed lipopolysaccharides from four strains of campylobacter jejuni and two strains of campylobacter coli. | lipopolysaccharides (lps) from four strains of campylobacter jejuni and two strains of c. coli were partially hydrolysed with 1% acetic acid. subsequent chloroform extraction led to the formation of a polysaccharide-containing aqueous layer, an interfacial material and a lipid a-containing chloroform layer. the polysaccharides contained the neutral sugars, amino sugars, 2-keto-3-deoxy-octonic acid, and part of the phosphorus present in the undegraded lps. the lipid as were made up of glucosamine ... | 1984 | 6527123 |
| antibacterial activities of nitrothiazole against campylobacter jejuni and campylobacter coli. | niridazole (ambilhar) and three other newly synthesized nitrothiazole derivatives were highly active against 19 microaerophilic campylobacters (minimum concentration required to inhibit 50% of strains [mic50], 0.0075 to 0.015 mg/liter). there were, however, considerable differences in the susceptibility among strains tested, and one nitrothiazole derivative was rather inactive (mic50, 2 mg/liter). nitroimidazole derivatives, such as metronidazole and tinidazole, were less active (mic50, 2 and 4 ... | 1984 | 6517542 |
| a pulmonary abnormality of newborn lambs. | nineteen pregnant ewes deliberately infected orally with campylobacter coli/jejuni at either 80 or 120 days gestation and accidentally subjected to a period of heat stress during the latter half of pregnancy produced clinically normal but growth-retarded lambs at term. a group of 7 pregnant ewes uninfected with campylobacter but subjected to the same heat stress produced lambs clinically and morphologically similar to the lambs of the other 2 groups. microscopically, the lungs from 11 lambs show ... | 1984 | 6512027 |
| improved biotyping schemes for campylobacter jejuni and campylobacter coli. | campylobacter jejuni (20 strains) and campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. the resulting biotyping schemes offer a greater degree of distinction among c. jejuni and c. coli strains than any of the other biotyping schemes previously described for these organisms. | 1984 | 6511880 |
| comparison of antimicrobial susceptibility patterns of campylobacter jejuni and campylobacter coli. | to determine whether employing antibiograms is useful to separate campylobacter jejuni and campylobacter coli, we determined the mics of 12 antibiotics for 104 human clinical strains and 74 swine strains. of 74 swine strains, 5 (7%) were hippurate positive, as were 93 (89%) of 104 human strains. the 12 antimicrobial agents tested were ampicillin, amoxicillin, clindamycin, chloramphenicol, erythromycin, furazolidone, norfloxacin, nalidixic acid, rosoxacin, rosaramicin, tetracycline, and sch 32063 ... | 1984 | 6508265 |
| does the occurrence of plasmids in campylobacter jejuni and campylobacter coli isolates correlate with antibiotic resistance? | 1984 | 6499842 | |
| campylobacter jejuni and campylobacter coli inoculation of neonatal calves. | three groups of neonatal calves were inoculated orally with pathogenic strains of campylobacter jejuni or c coli. the calves developed a mild, self-limiting enteritis characterized by thick mucoid feces. bacteremia and fecal shedding of campylobacter were sporadic in all inoculated calves. two groups of calves were killed 1 to 3 weeks after inoculation to study the pathogenesis of infection. postmortem culture of tissues revealed c jejuni or c coli most frequently in the ileum, cecum, colon, and ... | 1984 | 6497139 |
| plasmid content and pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model. | strains of campylobacter jejuni and c coli from human and animal sources were assessed for pathogenicity by their capability to produce abortions in pregnant guinea pigs. the pathogenicity rate varied from 7% to 87% for the c jejuni strains and from 0% to 53% for the c coli strains. plasmid carriage was not found to correlate with pathogenicity as determined by ability of the strains to produce abortions in the pregnant guinea pig model. | 1984 | 6497123 |
| new, extended biotyping scheme for campylobacter jejuni, campylobacter coli, and "campylobacter laridis". | a biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid h2s production, and dna hydrolysis was applied to 1,826 cultures of campylobacter jejuni, campylobacter coli and "campylobacter laridis" isolates from human and nonhuman sources. four biotypes were identified among c. jejuni: 57.3% of the isolates belonged to biotype i; 36.0%, to biotype ii; 4.0%, to biotype iii; and 2.7%, to biotype iv. c. coli organisms were differentiated into biotype i (67.0% ... | 1984 | 6490850 |
| differentiation of campylobacter species by protein banding patterns in polyacrylamide slab gels. | soluble protein extracts of 37 catalase-positive strains of campylobacter species were examined by polyacrylamide slab gel electrophoresis (page). electrophoretic banding patterns showed good correlation with biochemical tests and with available dna homology data in distinguishing species of campylobacter but did not differentiate subspecies or biotypes. page patterns indicated that campylobacter coli is a distinct species. furthermore, the page patterns indicated that c. jejuni and nalidixic ac ... | 1984 | 6490829 |
| isoprenoid quinone content and cellular fatty acid composition of campylobacter species. | a total of 36 strains of campylobacter species were examined for isoprenoid quinones and cellular fatty acids. the isoprenoid quinone content was determined by reverse-phase high-pressure liquid chromatography, and the fatty acids were determined by capillary gas-liquid chromatography. all campylobacter species contained menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-napthoquinone) as the major i ... | 1984 | 6470096 |
| [antimicrobial susceptibility of campylobacter jejuni and campylobacter coli isolated from human and animals in japan]. | 1984 | 6442333 | |
| chemical composition and biological activity of lipopolysaccharides prepared from type strains of campylobacter jejuni and campolybacter coli. | lipopolysaccharides (lps) were extracted from seven type strains of campylobacter jejuni and one type strain of campylobacter coli with 45 per cent aqueous phenol. the sugar components present in all lps were glucose, galactose, l-glycero-d-manno-heptose, 2-keto-3-deoxy-octulosonic acid and glucosamine. all but one lps contained galactosamine, and two strains contained in addition mannose. the fatty acids present were 3-hydroxy-tetradecanoic acid, n-hexadecanoic acid and trace amounts of n-tetra ... | 1984 | 6440410 |
| a comparison of microaerobic systems for the culture of campylobacter jejuni and campylobacter coli. | the relative ability of two commercial gas generating envelopes, the evacuation-replacement technique, and a candle jar, to produce a satisfactory microaerobic atmosphere for the culture of 45 campylobacter strains on non-selective medium and five selective media (skirrow's, modified butzler's blaser's, campy-bap and preston medium) was investigated quantitatively. a candle jar, and modified butzler's medium proved to be of limited use. the ability of four commercial gas generating envelopes to ... | 1983 | 6407829 |
| [detection of campylobacter jejuni and campylobacter coli in the routine laboratory]. | 1984 | 6393616 | |
| analysis of campylobacter jejuni antigens with monoclonal antibodies. | to develop monoclonal reagents for antigenic analysis and serotyping of campylobacter spp., hybridoma cell lines were produced by fusion of mouse myeloma cells and spleen cells from mice immunized with formalin-treated campylobacter jejuni organisms. an enzyme immunoassay was used for preliminary screening of the cell culture supernatants and ascites. twenty-nine clones which reacted with the immunogen were obtained. seven of these clones were positive in passive hemagglutination tests with shee ... | 1984 | 6365954 |
| correlation of an expanded direct fluorescent-antibody system with an established passive hemagglutination system for serogrouping strains of campylobacter jejuni and campylobacter coli. | rabbits were inoculated with whole, formalinized cells from eight passive hemagglutination reference strains of campylobacter. fluorescein isothiocyanate-labeled immunoglobulin g from these antisera defined seven new direct fluorescent-antibody serogroups of c. jejuni and one new serogroup of c. coli. this expanded the campylobacter direct fluorescent antibody system to include 17 serogroups of c. jejuni, 3 serogroups of c. coli, and 2 serogroups of c. fetus. we then compared the passive hemaggl ... | 1983 | 6358249 |
| an acid extract as a common antigen in campylobacter coli and campylobacter jejuni strains. | an acid extract prepared from a single strain of campylobacter jejuni reacted with rabbit antisera against 21 strains of c. jejuni and campylobacter coli. the immunogen is resistant to formalin and to exposure to 100 degrees c for 30 min. the antigenic extract may have practical applications in human serology. | 1983 | 6343426 |