Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [evaluation of genotypic and phenotypic methods for the differentiation of campylobacter jejuni and campylobacter coli clinical isolates from poland. ii. pfge, eric-pcr, pcr-flaa-rflp and mlst]. | campylobacter jejuni and campylobacter coli are leading cause of bacterial gastroenteritis in many developed countries. we compared pulsed-field gel electrophoresis (pfge), enterobacterial repetitive intergenic consensus (eric-pcr) and restriction fragment length polymorphisms of the flagellin gene (pcr-flaa-rflp) for distinguishing 128 c. jejuni isolates and 17 c. coli clinical isolates isolated between 2006 and 2007 in poland. we also analysed seven isolates from three c. jejuni family outbrea ... | 2009 | 19517816 |
| the dual-functioning fumarate reductase is the sole succinate:quinone reductase in campylobacter jejuni and is required for full host colonization. | campylobacter jejuni encodes all the enzymes necessary for a complete oxidative tricarboxylic acid (tca) cycle. because of its inability to utilize glucose, c. jejuni relies exclusively on amino acids as the source of reduced carbon, and they are incorporated into central carbon metabolism. the oxidation of succinate to fumarate is a key step in the oxidative tca cycle. c. jejuni encodes enzymes annotated as a fumarate reductase (cj0408 to cj0410) and a succinate dehydrogenase (cj0437 to cj0439) ... | 2009 | 19525346 |
| prevalence of thermotolerant campylobacter in partridges (perdix perdix). | to estimate the prevalence of thermotolerant campylobacter spp. in commercially reared partridges (perdix perdix) in southern italy. | 2009 | 19552770 |
| novel campylobacter lari-like bacteria from humans and molluscs: description of campylobacter peloridis sp. nov., campylobacter lari subsp. concheus subsp. nov. and campylobacter lari subsp. lari subsp. nov. | a polyphasic study was undertaken to clarify the taxonomic position of campylobacter lari-like strains isolated from shellfish and humans. the diversity within the strain collection was initially screened by means of fluorescent amplified fragment length polymorphism analysis and whole-cell protein electrophoresis, revealing the existence of two clusters distinct from c. lari and other campylobacter species. the divergence of these clusters was confirmed by phenotypic analysis and by 16s rrna an ... | 2009 | 19406805 |
| [evaluation of genotypic and phenotypic methods for the differentiation of campylobacter jejuni and campylobacter coli clinical isolates from poland. i. biotyping, resistance pattern, plasmid analysis, restriction enzyme analysis of plasmid]. | a total of 128 clinical isolates of c. jejuni and 17 clinical isolates of c. coli were characterized by their plasmid profiles, susceptibility to six antimicrobial agents and the lior biotypes. we also analyzed seven isolates from three c. jejuni family outbreaks and three isolates from one c. coli family outbreak. plasmids were found in 47 c. jejuni (36.7%) and 3 c. coli (17.6%) isolates. the sizes of plasmids detected ranged from about 3.3 to 89 kb. the majority of campylobacter isolates (n = ... | 2009 | 19517815 |
| prokaryotic protein glycosylation is rapidly expanding from "curiosity" to "ubiquity". | 2009 | 19621412 | |
| campylobacter immunity and coinfection following a large outbreak in a farming community. | an outbreak of campylobacteriosis affected approximately one-half of 165 people attending an annual farmers' dance in montrose, scotland, in november 2005. epidemiological investigations, including a cohort study (n = 164), identified chicken liver paté as the most likely vehicle of infection. paté preparation involved deliberate undercooking of chicken livers by flash-frying, followed by mechanical homogenization. typing of 32 campylobacter strains (isolated from submitted stools) by multilocus ... | 2009 | 19005146 |
| [determination of sensitivity to antibiotics of campylobacter jejuni and campylobacter coli isolated from human feces]. | one of the most important bacterial zoonosis is campylobacteriosis. human disease is mostly caused by thermophilic campylobacter spp: campylobacter jejuni (c. jejuni), campylobacter coli (c. coli), campylobacter lari (c. lari) and campylobacter upsakensis (campsalensis). campylobacteriosis is a mild and self-healing disorder. in patients with more severe and prolonged forms, an antibiotic treatment is recommended. recommended drugsare erythromycin, ciprofloxacin, tetracyclin, chloramphenicol and ... | 2009 | 19195264 |
| a single nucleotide change affects fur-dependent regulation of sodb in h. pylori. | helicobacter pylori is a significant human pathogen that has adapted to survive the many stresses found within the gastric environment. superoxide dismutase (sodb) is an important factor that helps h. pylori combat oxidative stress. sodb was previously shown to be repressed by the ferric uptake regulator (fur) in the absence of iron (apo-fur regulation) [1]. herein, we show that apo regulation is not fully conserved among all strains of h. pylori. apo-fur dependent changes in sodb expression are ... | 2009 | 19399190 |
| population structure of streptococcus oralis. | streptococcus oralis is a member of the normal human oral microbiota, capable of opportunistic pathogenicity; like related oral streptococci, it exhibits appreciable phenotypic and genetic variation. a multilocus sequence typing (mlst) scheme for s. oralis was developed and the resultant data analysed to examine the population structure of the species. analysis of 113 isolates, confirmed as belonging to the s. oralis/mitis group by 16s rrna gene sequencing, characterized the population as highly ... | 2009 | 19423627 |
| pyrazolopyrimidinediones are selective agents for helicobacter pylori that suppress growth through inhibition of glutamate racemase (muri). | pyrazolopyrimidinediones are a novel series of compounds that inhibit growth of helicobacter pylori specifically. using a variety of methods, advanced analogues were shown to suppress the growth of h. pylori through the inhibition of glutamate racemase, an essential enzyme in peptidoglycan biosynthesis. the high degree of selectivity of the series for h. pylori makes these compounds attractive candidates for novel h. pylori-selective therapy. | 2009 | 19433553 |
| application of a group ii campylobacter bacteriophage to reduce strains of campylobacter jejuni and campylobacter coli colonizing broiler chickens. | members of the genus campylobacter are frequently responsible for human enteric disease worldwide. persistent campylobacter contamination of poultry meat is a common problem that represents a significant food safety risk through the consumption of undercooked poultry meat or through cross-contamination of other foods during the preparation of poultry. bacteriophage therapy is one possible means by which this colonization of poultry could be controlled, thus limiting the entry of campylobacter in ... | 2009 | 19435220 |
| production and characterization of a monoclonal antibody to campylobacter jejuni. | campylobacter species are a group of spiral-shaped bacteria that can cause disease in humans and animals. we developed a high-affinity monoclonal antibody (mab) probe that recognizes campylobacter jejuni cells. cell suspensions grown under microaerobic conditions at 42 degrees c for 20 h on bolton agar plates with lysed horse blood were used as live and heat-killed preparations, centrifuged at 8,000 x g for 20 min, and resuspended in carbonate buffer (ph 9.6) for coating on the enzyme-linked imm ... | 2009 | 19435241 |
| transmission of foodborne zoonotic pathogens to riparian areas by grazing sheep. | the objective of this study was to determine if sheep grazing near riparian areas on pasture in ontario are an important risk factor for the contamination of water with specific foodborne pathogens. ten ontario sheep farms were visited weekly for 12 wk during the summer of 2005. samples of feces, soil, and water were collected and analyzed for the presence of escherichia coli o157:h7, salmonella spp., campylobacter jejuni and c. coli, and yersinia enterocolitica, by bacteriological identificatio ... | 2009 | 19436581 |
| diversity and habitat specificity of free-living protozoa in commercial poultry houses. | despite stringent biosecurity measures, infections by bacterial food pathogens such as campylobacter are a recurrent problem in industrial poultry houses. as the main transmission route remains unclear, persistence of these infections has been linked to bacterial survival and possibly multiplication within protozoan vectors. to date, however, virtually no information is available on the diversity and occurrence of free-living protozoa in these environments. using a combination of microscopic ana ... | 2009 | 19124593 |
| structures of acrr and cmer: insight into the mechanisms of transcriptional repression and multi-drug recognition in the tetr family of regulators. | the transcriptional regulators of the tetr family act as chemical sensors to monitor the cellular environment in many bacterial species. to perform this function, members of the tetr family harbor a diverse ligand-binding domain capable of recognizing the same series of compounds as the transporters they regulate. many of the regulators can be induced by a wide array of structurally unrelated compounds. binding of these structurally unrelated ligands to the regulator results in a conformational ... | 2009 | 19130905 |
| structures of acrr and cmer: insight into the mechanisms of transcriptional repression and multi-drug recognition in the tetr family of regulators. | the transcriptional regulators of the tetr family act as chemical sensors to monitor the cellular environment in many bacterial species. to perform this function, members of the tetr family harbor a diverse ligand-binding domain capable of recognizing the same series of compounds as the transporters they regulate. many of the regulators can be induced by a wide array of structurally unrelated compounds. binding of these structurally unrelated ligands to the regulator results in a conformational ... | 2009 | 19130905 |
| comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of campylobacter jejuni and campylobacter coli in naturally contaminated chicken meat samples. | we investigated the efficacy of a loop-mediated isothermal amplification (lamp) assay for detection of chicken meat samples naturally contaminated with campylobacter jejuni and campylobacter coli. a total of 144 preston enrichment broth cultures from chicken meat samples were assessed by using the lamp assay and conventional culture methods, which consist of a combination of preston enrichment culturing and plating onto butzler and modified charcoal cefoperazone deoxycholate agars. compared with ... | 2009 | 19139242 |
| recombineering reveals a diverse collection of ribosomal proteins l4 and l22 that confer resistance to macrolide antibiotics. | mutations in ribosomal proteins l4 and l22 confer resistance to erythromycin and other macrolide antibiotics in a variety of bacteria. l4 and l22 have elongated loops whose tips converge in the peptide exit tunnel near the macrolide-binding site, and resistance mutations typically affect residues within these loops. here, we used bacteriophage lambda red-mediated recombination, or "recombineering," to uncover new l4 and l22 alleles that confer macrolide resistance in escherichia coli. we randomi ... | 2009 | 19150357 |
| a dnase encoded by integrated element cjie1 inhibits natural transformation of campylobacter jejuni. | the species campylobacter jejuni is considered naturally competent for dna uptake and displays strong genetic diversity. nevertheless, nonnaturally transformable strains and several relatively stable clonal lineages exist. in the present study, the molecular mechanism responsible for the nonnatural transformability of a subset of c. jejuni strains was investigated. comparative genome hybridization indicated that c. jejuni mu-like prophage integrated element 1 (cjie1) was more abundant in nonnatu ... | 2009 | 19151136 |
| incidence and risk factors for community-acquired acute gastroenteritis in north-west germany in 2004. | in developed countries, acute gastroenteritis (age) is a major source of morbidity. however, only a few studies have estimated its incidence and the associated medical burden. this population-based study determined the incidence of community-acquired age patients seeking medical care and the relative role of various pathogens. stool samples from patients with age presenting to a general practitioner (gp), pediatrician, or specialist in internal medicine for that reason were screened for various ... | 2009 | 19319582 |
| identification of novel carbohydrate modifications on campylobacter jejuni 11168 flagellin using metabolomics-based approaches. | it is well known that the flagellin of campylobacter jejuni is extensively glycosylated by pseudaminic acid and the related acetamindino derivative, in addition to flagellin glycosylation being essential for motility and colonization of host cells. recently, the use of metabolomics permitted the unequivocal characterization of unique flagellin modifications in campylobacter, including novel legionaminic acid sugars in campylobacter coli, which had been impossible to ascertain in earlier studies ... | 2009 | 19154343 |
| severe diarrhoea caused by highly ciprofloxacin-susceptible campylobacter isolates. | the impact of fluoroquinolone resistance of campylobacter jejuni and campylobacter coli isolates on the outcome of the disease in sporadic campylobacter infections of finnish individuals was studied. questionnaires were sent, during a 6-month study period, to patients who were stool culture-positive for campylobacter spp. in total, 192 returned questionnaires were analysed and assessed, together with the susceptibility data of the respective bacterial isolates. only one (2%) of the domestic, but ... | 2009 | 19154485 |
| temporal patterns and risk factors for escherichia coli o157 and campylobacter spp, in young cattle. | escherichia coli o157 and campylobacter jejuni and campylobacter coli are zoonotic pathogens originating from farm animals. cattle are the main reservoir for e. coli o157 and also contribute to human cases of campylobacteriosis through contaminated milk, direct contact, and environmental contamination. thirty groups of young cattle on 30 farms were observed for 7 months and sampled on 4 to 6 separate occasions for e. coli o157 and c. jejuni/coli to characterize shedding patterns and identify ris ... | 2009 | 19343935 |
| campylobacter coli naturally resistant to elevated levels of gentamicin as a marker strain in poultry research. | campylobacter inoculation studies are limited without a suitable marker strain. the lurpose of this study was to screen campylobacter strains (n=2073) obtained from poultry carcass rinses through the centers for disease control and prevention's national antimicrobial resistant monitoring system for resistance to gentamicin and evaluate one strain's efficacy as a marker. a c. coli strain was found resistant to gentamicin at >32 microg/ml. gentamicin was incorporated into media (campy-cefex agar, ... | 2009 | 19610342 |
| comparative studies of campylobacter jejuni genomic diversity reveal the importance of core and dispensable genes in the biology of this enigmatic food-borne pathogen. | mlst, dna microarrays, and genome sequencing has allowed for a greater understanding of the metabolic capacity and epidemiology of campylobacter jejuni. while strain-specific genes may provide an isolate a selective advantage in environments and contribute to the organism's pathogenicity, recent work indicates that c. jejuni pathogenicity is dictated by variations in the nucleotide sequence of core genes. challenges facing c. jejuni researchers include determining (a) the degree to which genomic ... | 2009 | 19346123 |
| campylobacter colonization of the turkey intestine in the context of microbial community development. | patterns of microbial community dynamics in the turkey intestine were examined. every week of the 18-week production cycle, cecal bacterial communities and campylobacter loads were examined from five birds for each of two flocks. molecular fingerprinting via the automated ribosomal intergenic spacer analysis (arisa) and terminal restriction fragment length polymorphism (t-rflp) of the cecal samples revealed that microbial communities changed in a time-dependent manner, and during both trials the ... | 2009 | 19346343 |
| genomic insights into the convergence and pathogenicity factors of campylobacter jejuni and campylobacter coli species. | whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. a recent report implied that not only may the former be true but also, in fact, the clearly distinct campylobacter jejuni and campylobacter coli species may be converging as a consequence of increased interspecies gene flow fostered, presumably, by the recent invasion of an overlapping ecological niche (s. k. sheppard, n. d. mccarthy, ... | 2009 | 19617370 |
| a longitudinal study of campylobacter distribution in a turkey production chain. | campylobacter is the most common cause of bacterial enteritis worldwide. handling and eating of contaminated poultry meat has considered as one of the risk factors for human campylobacteriosis. campylobacter contamination can occur at all stages of a poultry production cycle. the objective of this study was to determine the occurrence of campylobacter during a complete turkey production cycle which lasts for 1,5 years of time. for detection of campylobacter, a conventional culture method was com ... | 2009 | 19348687 |
| functional characterization of excision repair and reca-dependent recombinational dna repair in campylobacter jejuni. | the presence and functionality of dna repair mechanisms in campylobacter jejuni are largely unknown. in silico analysis of the complete translated genome of c. jejuni nctc 11168 suggests the presence of genes involved in methyl-directed mismatch repair (mmr), nucleotide excision repair, base excision repair (ber), and recombinational repair. to assess the functionality of these putative repair mechanisms in c. jejuni, muts, uvrb, ung, and reca knockout mutants were constructed and analyzed for t ... | 2009 | 19376866 |
| erythromycin resistance by l4/l22 mutations and resistance masking by drug efflux pump deficiency. | we characterized the effects of classical erythromycin resistance mutations in ribosomal proteins l4 and l22 of the large ribosomal subunit on the kinetics of erythromycin binding. our data are consistent with a mechanism in which the macrolide erythromycin enters and exits the ribosome through the nascent peptide exit tunnel, and suggest that these mutations both impair passive transport through the tunnel and distort the erythromycin-binding site. the growth-inhibitory action of erythromycin w ... | 2009 | 19197244 |
| inter- and intra-genomic heterogeneity of the intervening sequence in the 23s ribosomal rna gene of campylobacter jejuni and campylobacter coli. | an intervening sequence (ivs) can be present or absent in the 23s rrna of campylobacter jejuni and campylobacter coli. as part of a survey, we used a polymerase chain reaction (pcr) assay to detect the presence of the ivs in 43 isolates of c. coli and 82 isolates of c. jejuni. an ivs was present in 40 (93.0%) of the c. coli and only 34 (41.5%) of the c. jejuni isolates. twelve (27.9%) of the c. coli isolates and seven (8.5%) of the c. jejuni isolates resulted in two polymerase chain reaction pro ... | 2009 | 19201124 |
| campylobacter jejuni in penguins, antarctica. | 2009 | 19402996 | |
| prevalence of campylobacter jejuni and campylobacter coli in market-weight turkeys on-farm and at slaughter. | to monitor the effects of feed withdrawal on the prevalence of campylobacter, market-weight turkeys from six farms were examined before and after perimarketing events (feed withdrawal, transport, and holding at the slaughterhouse). prior to transport, birds (n = 30 per farm) were slaughtered on-farm, and viscera (crops, duodenum, jejunum, ileum, colon, ceca, gallbladder, and spleen) were removed on the premises. within ca. 48 h, cohorts (n = 30 per farm) from the same flock were transported to a ... | 2009 | 19205462 |
| genetic diversity of campylobacter on broiler carcasses collected preevisceration and postchill in 17 u.s. poultry processing plants. | campylobacter jejuni and campylobacter coli are the most important human enteropathogens among the campylobacters. the objective of this study was to determine how diversity in campylobacter populations found on chicken carcasses collected from 17 broiler processing plants in the united states is impacted by processing. genetic diversity was determined for up to four isolates per carcass by sequencing the short variable region (svr) of the flaa locus. on 70% of campylobacter-positive carcasses, ... | 2009 | 19205463 |
| genotyping of campylobacter coli isolated from humans and retail meats using multilocus sequence typing and pulsed-field gel electrophoresis. | to determine the antimicrobial resistant profiles and clonality of campylobacter coli isolated from clinically ill humans and retail meats. | 2009 | 19226383 |
| a pan-european survey of antimicrobial susceptibility towards human-use antimicrobial drugs among zoonotic and commensal enteric bacteria isolated from healthy food-producing animals. | the aim of the study was to study antimicrobial susceptibility in escherichia coli, salmonella, campylobacter and enterococcus recovered from chickens, pigs and cattle using uniform methodology. | 2009 | 19233897 |
| antiganglioside antibodies and their pathophysiological effects on guillain-barré syndrome and related disorders--a review. | guillain-barré syndrome (gbs) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. infection with micro-organisms, including campylobacter jejuni (c. jejuni), haemophilus influenzae, and cytomegalovirus (cmv), is recognized as a main triggering event for the disease. lipooligosaccharide (los) genes are responsible for the formation of human ganglioside-like los structures in infectious micro-organisms that can induce gbs. molecular mimicry of loss on the surface ... | 2009 | 19240270 |
| evaluation of procedures for outer membrane isolation from campylobacter jejuni. | although infection with campylobacter jejuni is one of the leading causes of gastroenteritis worldwide, relatively little is known about the factors that are required to elicit a protective immune response. the need for a vaccine against this pathogen is well recognized and a number of vaccine candidates have been tested with varying degrees of success; however, there is still a lack of a suitable vaccine. to gain a better understanding of the outer-membrane protein components of this organism, ... | 2009 | 19246768 |
| antibiotic resistance in campylobacter: emergence, transmission and persistence. | campylobacter is a leading foodborne bacterial pathogen, which causes gastroenteritis in humans. this pathogenic organism is increasingly resistant to antibiotics, especially fluoroquinolones and macrolides, which are the most frequently used antimicrobials for the treatment of campylobacteriosis when clinical therapy is warranted. as a zoonotic pathogen, campylobacter has a broad animal reservoir and infects humans via contaminated food, water or milk. antibiotic usage in both animal agricultur ... | 2009 | 19257846 |
| attribution of campylobacter infections in northeast scotland to specific sources by use of multilocus sequence typing. | we show that a higher incidence of campylobacteriosis is found in young children (age, <5 years) living in rural, compared with urban, areas. association of this difference with particular animal sources was evaluated using multilocus sequence typing. this evaluation was achieved by comparing campylobacter isolates originating from these children, retail poultry, and a range of animal sources by use of source attribution and phylogenetic analysis methods. the results indicate that chicken is a m ... | 2009 | 19265482 |
| campylobacter genotypes from food animals, environmental sources and clinical disease in scotland 2005/6. | a nationwide multi-locus sequence typing (mlst) survey was implemented to analyze patterns of host association among campylobacter jejuni and campylobacter coli isolates from clinical disease in scotland (july 2005-september 2006), food animals (chickens, cattle, sheep, pigs and turkey), non-food animals (wild birds) and the environment. sequence types (sts) were determined for 5247 clinical isolates and 999 from potential disease sources (augmented with 2420 published sts). certain sts were ove ... | 2009 | 19269051 |
| campylobacter genotyping to determine the source of human infection. | campylobacter species cause a high proportion of bacterial gastroenteritis cases and are a significant burden on health care systems and economies worldwide; however, the relative contributions of the various possible sources of infection in humans are unclear. | 2009 | 19275496 |
| the cmp-legionaminic acid pathway in campylobacter: biosynthesis involving novel gdp-linked precursors. | the sialic acid-like sugar 5,7-diacetamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-nonulosonic acid, or legion-aminic acid, is found as a virulence-associated cell-surface glycoconjugate in the gram-negative bacteria legionella pneumophila and campylobacter coli. l. pneumophila serogroup 1 strains, causative agents of legionnaire's disease, contain an alpha2,4-linked homopolymer of legionaminic acid within their lipopolysaccharide o-chains, whereas the gastrointestinal pathogen c. coli modifies i ... | 2009 | 19282391 |
| molecular epidemiology and characterization of campylobacter spp. isolated from wild bird populations in northern england. | campylobacter infections have been reported at prevalences ranging from 2 to 50% in a range of wild bird species, although there have been few studies that have investigated the molecular epidemiology of campylobacter spp. consequently, whether wild birds are a source of infection in humans or domestic livestock or are mainly recipients of domestic animal strains and whether separate cycles of infection occur remain unknown. to address these questions, serial cross-sectional surveys of wild bird ... | 2009 | 19286781 |
| regulation of superoxide dismutase (sod) genes by sara in staphylococcus aureus. | the scavenging of reactive oxygen species (ros) within cells is regulated by several interacting factors, including transcriptional regulators. involvement of sara family genes in the regulation of proteins involved in the scavenging of ros is largely unknown. in this report, we show that under aerobic conditions, the levels of sodm and soda transcription, in particular the sodm transcript, are markedly enhanced in the sara mutant among the tested sara family mutants. increased levels of sod exp ... | 2009 | 19286803 |
| independent evolution of neurotoxin and flagellar genetic loci in proteolytic clostridium botulinum. | proteolytic clostridium botulinum is the causative agent of botulism, a severe neuroparalytic illness. given the severity of botulism, surprisingly little is known of the population structure, biology, phylogeny or evolution of c. botulinum. the recent determination of the genome sequence of c. botulinum has allowed comparative genomic indexing using a dna microarray. | 2009 | 19298644 |
| campylobacter jejuni glycosylation island important in cell charge, legionaminic acid biosynthesis, and colonization of chickens. | previously, we identified five genes (cj1321 to cj1326, of which cj1325 and cj1326 are a single gene) in the o-linked flagellin glycosylation island that are highly prevalent in campylobacter jejuni isolates from chickens. we report mutagenesis, functional, and structural data to confirm that this locus, and cj1324 in particular, has a significant contributory role in the colonization of chickens by c. jejuni. a motile deltacj1324 mutant with intact flagella was considerably less hydrophobic and ... | 2009 | 19307210 |
| survival at refrigeration and freezing temperatures of campylobacter coli and campylobacter jejuni on chicken skin applied as axenic and mixed inoculums. | campylobacter is considered to be the most common cause of bacterial diarrhoeal illness in the developed world. many cases are thought to be acquired from consumption of undercooked poultry. the aim of this study was to compare the effect of the rate of cooling on the survival, at 4 degrees c and -20 degrees c, of campylobacter coli and campylobacter jejuni strains, inoculated on chicken skin from axenic culture or as mixed inoculums. strains chilled in a domestic refrigerator varied in their to ... | 2009 | 19324444 |
| rapid evolution and the importance of recombination to the gastroenteric pathogen campylobacter jejuni. | responsible for the majority of bacterial gastroenteritis in the developed world, campylobacter jejuni is a pervasive pathogen of humans and animals, but its evolution is obscure. in this paper, we exploit contemporary genetic diversity and empirical evidence to piece together the evolutionary history of c. jejuni and quantify its evolutionary potential. our combined population genetics-phylogenetics approach reveals a surprising picture. campylobacter jejuni is a rapidly evolving species, subje ... | 2009 | 19008526 |
| roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of campylobacter jejuni. | this study is aimed to determine the role of capsular polysaccharide (cps) and lipooligosaccharide (los) in modulating antimicrobial resistance and natural transformation of campylobacter jejuni, an important food-borne human pathogen. | 2009 | 19147521 |
| 23s rrna gene mutations contributing to macrolide resistance in campylobacter jejuni and campylobacter coli. | the genetic basis of macrolide resistance in campylobacter coli (n = 17) and c. jejuni (n = 35) isolates previously subjected to in vivo selective pressure was investigated to determine if the number of copies of 23s rrna genes with macrolide-associated mutations affects the minimum inhibitory concentration (mic) of macrolides. sequence data for domain v of the 23s rrna gene revealed that two macrolide-resistant c. coli isolates had adenine-->guanine transitions at position 2059 (a2059g, escheri ... | 2009 | 19014274 |
| survival of campylobacter spp. in bovine faeces on pasture. | to determine the survival on pasture of campylobacter spp. naturally present in bovine faeces and compare this with a previously published study using laboratory-cultured campylobacter spp. | 2009 | 19018956 |
| antimicrobial susceptibility profiles and strain type diversity of campylobacter jejuni isolates from turkeys in eastern north carolina. | campylobacter jejuni is one of the most common bacterial causes of human gastroenteritis, and recent findings suggest that turkeys are an important reservoir for this organism. in this study, 80 c. jejuni isolates from eastern north carolina were characterized for resistance to nine antimicrobials, and strain types were determined by fla typing, pulsed-field gel electrophoresis (pfge) with smai and kpni, and (for 41 isolates) multilocus sequence typing (mlst). pfge analysis suggested that many o ... | 2009 | 19028914 |
| antimicrobial susceptibility profiles and strain type diversity of campylobacter jejuni isolates from turkeys in eastern north carolina. | campylobacter jejuni is one of the most common bacterial causes of human gastroenteritis, and recent findings suggest that turkeys are an important reservoir for this organism. in this study, 80 c. jejuni isolates from eastern north carolina were characterized for resistance to nine antimicrobials, and strain types were determined by fla typing, pulsed-field gel electrophoresis (pfge) with smai and kpni, and (for 41 isolates) multilocus sequence typing (mlst). pfge analysis suggested that many o ... | 2009 | 19028914 |
| quantifying transmission of campylobacter jejuni in commercial broiler flocks. | since meat from poultry colonized with campylobacter spp. is a major cause of bacterial gastroenteritis, human exposure should be reduced by, among other things, prevention of colonization of broiler flocks. to obtain more insight into possible sources of introduction of campylobacter into broiler flocks, it is essential to estimate the moment that the first bird in a flock is colonized. if the rate of transmission within a flock were known, such an estimate could be determined from the change i ... | 2009 | 19047389 |
| quantifying transmission of campylobacter jejuni in commercial broiler flocks. | since meat from poultry colonized with campylobacter spp. is a major cause of bacterial gastroenteritis, human exposure should be reduced by, among other things, prevention of colonization of broiler flocks. to obtain more insight into possible sources of introduction of campylobacter into broiler flocks, it is essential to estimate the moment that the first bird in a flock is colonized. if the rate of transmission within a flock were known, such an estimate could be determined from the change i ... | 2009 | 19047389 |
| oxidative stress-induced peptidoglycan deacetylase in helicobacter pylori. | structural modification of peptidoglycan (pg) is one of the mechanisms that pathogenic bacteria use to evade the host innate immune system. for the noninvasive human gastric pathogen helicobacter pylori, pg delivery to the host cells is one trigger of the immune response. h. pylori hp310 was markedly up-expressed upon cell exposure to oxidative stress. however, disruption of hp310 did not produce a phenotype distinguishable from the parent, including oxidative stress resistance characteristics. ... | 2009 | 19147492 |
| campylobacter and arcobacter species sensitivity to commercial orange oil fractions. | seven orange oil fractions were screened for their ability to inhibit the growth of selected campylobacter and arcobacter spp. using the standard agar-disk diffusion assay. cold pressed (cp) terpeneless valencia orange oil was found to be the most inhibitory to both campylobacter jejuni and campylobacter coli, exhibiting maximum zones of inhibition up to 80+/-0.0 mm. five-fold concentrated valencia oil and distilled d-limonene resulted in campylobacter inhibition zones ranging from 11.0+/-1.4 to ... | 2009 | 19070381 |
| a campylobacter jejuni znua orthologue is essential for growth in low-zinc environments and chick colonization. | campylobacter jejuni infection is a leading cause of bacterial gastroenteritis in the united states and is acquired primarily through the ingestion of contaminated poultry products. here, we describe the c. jejuni orthologue of znua in other gram-negative bacteria. znua (cj0143c) is the periplasmic component of a putative zinc abc transport system and is encoded on a zinc-dependent operon with cj0142c and cj0141c, which encode the other two likely components of the transport system of c. jejuni. ... | 2009 | 19103921 |
| a campylobacter jejuni znua orthologue is essential for growth in low-zinc environments and chick colonization. | campylobacter jejuni infection is a leading cause of bacterial gastroenteritis in the united states and is acquired primarily through the ingestion of contaminated poultry products. here, we describe the c. jejuni orthologue of znua in other gram-negative bacteria. znua (cj0143c) is the periplasmic component of a putative zinc abc transport system and is encoded on a zinc-dependent operon with cj0142c and cj0141c, which encode the other two likely components of the transport system of c. jejuni. ... | 2009 | 19103921 |
| antimicrobial activity of prulifloxacin tested against a worldwide collection of gastroenteritis-producing pathogens, including those causing traveler's diarrhea. | prulifloxacin, the prodrug of ulifloxacin (active component), is a newer fluoroquinolone with broad activity against enteric and nonenteric gram-negative bacilli. ulifloxacin and other oral comparator agents were tested for activity against 582 gastroenteritis strains from global surveillance studies. ulifloxacin was highly active against escherichia coli, salmonella spp., shigella spp., yersinia spp., vibrio spp., aeromonas spp., and plesiomonas spp. (mic(50)s and mic(90)s, <or=0.03 microg/ml a ... | 2009 | 19114678 |
| antimicrobial activity of prulifloxacin tested against a worldwide collection of gastroenteritis-producing pathogens, including those causing traveler's diarrhea. | prulifloxacin, the prodrug of ulifloxacin (active component), is a newer fluoroquinolone with broad activity against enteric and nonenteric gram-negative bacilli. ulifloxacin and other oral comparator agents were tested for activity against 582 gastroenteritis strains from global surveillance studies. ulifloxacin was highly active against escherichia coli, salmonella spp., shigella spp., yersinia spp., vibrio spp., aeromonas spp., and plesiomonas spp. (mic(50)s and mic(90)s, <or=0.03 microg/ml a ... | 2009 | 19114678 |
| campylobacter spp among children with acute diarrhea attending mulago hospital in kampala--uganda. | campylobacter infections occur worldwide. a recent study in kampala, uganda, found that 87% of broiler chickens had campylobacter jejuni; these are potential source of human infection. isolation rate in developing countries is between 5-35%. this study aimed at finding prevalence of children with campylobacter infection among children with acute diarrhea attending mulago hospital. | 2009 | 20589152 |
| multiplex pcr to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. | sepsis is caused by a heterogeneous group of infectious etiologies. early diagnosis and the provision of appropriate antimicrobial therapy correlate with positive clinical outcomes. current microbiological techniques are limited in their diagnostic capacities and timeliness. multiplex pcr has the potential to rapidly identify bloodstream infections and fill this diagnostic gap. we identified patients from two large academic hospital emergency departments with suspected sepsis. the results of a m ... | 2009 | 19846634 |
| multiplex pcr to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. | sepsis is caused by a heterogeneous group of infectious etiologies. early diagnosis and the provision of appropriate antimicrobial therapy correlate with positive clinical outcomes. current microbiological techniques are limited in their diagnostic capacities and timeliness. multiplex pcr has the potential to rapidly identify bloodstream infections and fill this diagnostic gap. we identified patients from two large academic hospital emergency departments with suspected sepsis. the results of a m ... | 2009 | 19846634 |
| occurrence of campylobacter jejuni and campylobacter coli and their biotypes in beef and dairy cattle from the south of chile. | the prevalence of campylobacter jejuni and campylobacter coli and their biotypes in beef and dairy cattle from the south of chile was established. campylobacter were statistically more prevalent among beef cattle (35.9%) than among dairy cattle (21.3%), being c. jejuni the species most frequently isolated. | 2009 | 24031386 |
| rapid identification of bacterial pathogens using a pcr- and microarray-based assay. | during the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. we have developed a method based on a modified broad-range pcr and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. the broad-range pcr primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrb and pare. the primer design a ... | 2009 | 19664269 |
| comprehensive and rapid real-time pcr analysis of 21 foodborne outbreaks. | a set of four duplex sybr green i pcr (sg-pcr) assay combined with dna extraction using qiaamp dna stool mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks. the causative pathogens were detected in almost all cases in 2 hours or less. the first run was for the detection of 8 main foodborne pathogens in 5 stool specimens within 2 hours and the second run was for the detection of other unusual suspect pathogens within a further 45 minutes. after 2 to 4 days, ... | 2009 | 20016673 |
| drug efflux pump deficiency and drug target resistance masking in growing bacteria. | recent experiments have shown that drug efflux pump deficiency not only increases the susceptibility of pathogens to antibiotics, but also seems to "mask" the effects of mutations, that decrease the affinities of drugs to their intracellular targets, on the growth rates of drug-exposed bacteria. that is, in the presence of drugs, the growth rates of drug-exposed wt and target mutated strains are the same in a drug efflux pump deficient background, but the mutants grow faster than wt in a drug ef ... | 2009 | 19416855 |
| antimicrobial drug resistance as determined by the e-test in campylobacter jejuni, c. coli, and c. lari isolates from the ceca of broiler and layer chickens in grenada. | one hundred and twenty five chickens from grenada, consisting of 77 broilers and 48 layers were examined for carriage of thermophilic campylobacters in their ceca by culture. seventy nine percent of chickens were positive for campylobacters, with an isolation rate of 93.5% for broilers and 56.3% for layers, the difference being significant. sixty-four pure cultures comprising 39 campylobacter coli, 21 campylobacter jejuni, and 4 campyilobacter lari isolates were tested for their resistance again ... | 2009 | 18329712 |
| prevalence of campylobacter jejuni and its resistance to antibiotics in poultry in the czech republic. | thermotolerant campylobacter spp., in particular campylobacter jejuni, are among the most frequently identified pathogens, found to be causing human gastrointestinal infections in europe, with the czech republic being no exception. the presented work aimed at assessing results of the first nationwide monitoring of prevalence and antibiotic resistance of campylobacter spp. in broiler flocks in the czech republic, including a comparison of antibiotic resistance of c. jejuni isolates collected from ... | 2009 | 18771516 |
| risk factors for campylobacter jejuni and campylobacter coli in young cattle on english and welsh farms. | campylobacter jejuni and c. coli are the most prevalent causes of bacterial diarrhoea in most of the western world. in great britain, the source remains unknown for the majority of cases, though poultry is considered the main source of infection. molecular typing methods identify cattle as a potential source of a proportion of the non-source-attributed cases, mainly through direct contact, environmental contamination or milk, but little is known about the epidemiology of campylobacter in cattle. ... | 2009 | 18835052 |
| can campylobacter coli induce guillain-barré syndrome? | 2009 | 19002726 | |
| initial studies using aliphatic beta-nitro alcohols for therapeutic corneal cross-linking. | corneal collagen cross-linking through uva-riboflavin photochemistry (uvar) has been shown to be an effective treatment for keratoconus and related keratectasias. in recent studies using sclera, the authors observed that short-chain aliphatic beta-nitro alcohols can cross-link collagenous tissue under physiologic conditions. thus, this study was undertaken to evaluate these agents as potential pharmacologic alternatives to uvar. | 2009 | 18836172 |
| initial studies using aliphatic beta-nitro alcohols for therapeutic corneal cross-linking. | corneal collagen cross-linking through uva-riboflavin photochemistry (uvar) has been shown to be an effective treatment for keratoconus and related keratectasias. in recent studies using sclera, the authors observed that short-chain aliphatic beta-nitro alcohols can cross-link collagenous tissue under physiologic conditions. thus, this study was undertaken to evaluate these agents as potential pharmacologic alternatives to uvar. | 2009 | 18836172 |
| dynamics of campylobacter colonization of a natural host, sturnus vulgaris (european starling). | wild european starlings (sturnus vulgaris) shed campylobacter at high rates, suggesting that they may be a source of human and farm animal infection. a survey of campylobacter shedding of 957 wild starlings was undertaken by culture of faecal specimens and genetic analysis of the campylobacters isolated: shedding rates were 30.6% for campylobacter jejuni, 0.6% for c. coli and 6.3% for c. lari. genotyping by multilocus sequence typing (mlst) and antigen sequence typing established that these bact ... | 2009 | 18826435 |
| campylobacter immunity and coinfection following a large outbreak in a farming community. | an outbreak of campylobacteriosis affected approximately one-half of 165 people attending an annual farmers' dance in montrose, scotland, in november 2005. epidemiological investigations, including a cohort study (n = 164), identified chicken liver paté as the most likely vehicle of infection. paté preparation involved deliberate undercooking of chicken livers by flash-frying, followed by mechanical homogenization. typing of 32 campylobacter strains (isolated from submitted stools) by multilocus ... | 2009 | 19005146 |
| detection of escherichia coli, salmonella spp., shigella spp., yersinia enterocolitica, vibrio cholerae, and campylobacter spp. enteropathogens by 3-reaction multiplex polymerase chain reaction. | the magnitude of bacterial diarrhea in developing countries is largely unknown because affordable detection methods are not available. we have developed a polymerase chain reaction (pcr)-based assay for use in areas with limited resources to screen for diarrheogenic strains from clinical isolates. to simplify the assay and minimize reagents, our method implemented the use of plasmids rather than bacteria as template controls and the use of bacterial suspensions or crude dna preparations rather t ... | 2009 | 18990527 |
| detection of escherichia coli, salmonella spp., shigella spp., yersinia enterocolitica, vibrio cholerae, and campylobacter spp. enteropathogens by 3-reaction multiplex polymerase chain reaction. | the magnitude of bacterial diarrhea in developing countries is largely unknown because affordable detection methods are not available. we have developed a polymerase chain reaction (pcr)-based assay for use in areas with limited resources to screen for diarrheogenic strains from clinical isolates. to simplify the assay and minimize reagents, our method implemented the use of plasmids rather than bacteria as template controls and the use of bacterial suspensions or crude dna preparations rather t ... | 2009 | 18990527 |
| demographic determinants for campylobacter infection in england and wales: implications for future epidemiological studies. | despite a significant public health burden the epidemiology of human campylobacter infection remains blurred. the identification of demographic determinants for campylobacter infection is therefore essential for identifying potential areas for intervention. demographic data from an active, population-based sentinel surveillance system for campylobacter infection (from 2000 until 2003, n=15 907) were compared with appropriate denominator data from the 2001 united kingdom census. incidence was hig ... | 2008 | 19000328 |
| extended sequence typing of campylobacter spp., united kingdom. | supplementing campylobacter spp. multilocus sequence typing with nucleotide sequence typing of 3 antigen genes increased the discriminatory index achieved from 0.975 to 0.992 among 620 clinical isolates from oxfordshire, united kingdom. this enhanced typing scheme enabled identification of clusters and retained data required for long-range epidemiologic comparisons of isolates. | 2008 | 18826829 |
| identification of nine sequence types of the 16s rrna genes of campylobacter jejuni subsp. jejuni isolated from broilers. | campylobacter is the most commonly reported bacterial cause of enteritis in humans in the eu member states and other industrialized countries. one significant source of infection is broilers and consumption of undercooked broiler meat. campylobacter jejuni is the campylobacter sp. predominantly found in infected humans and colonized broilers. sequence analysis of the 16s rrna gene is very useful for identification of bacteria to genus and species level. the objectives in this study were to deter ... | 2008 | 18492293 |
| pediatric campylobacteriosis in northern taiwan from 2003 to 2005. | there has been a marked increase in the incidence of, and concern regarding, human campylobacter jejuni and c. coli infections worldwide during the last decade. as the highest infectious disease control apparatus in taiwan, we aimed to describe the character of campylobacter isolates from infected children, as well as basic information about the patients, from december 2003 to february 2005. | 2008 | 18973701 |
| identification of campylobacter jejuni proteins recognized by maternal antibodies of chickens. | campylobacter jejuni is one of the leading bacterial causes of food-borne gastroenteritis. infection with c. jejuni is frequently acquired through the consumption of undercooked poultry or foods cross-contaminated with raw poultry. given the importance of poultry as a reservoir for campylobacter organisms, investigators have performed studies to understand the protective role of maternal antibodies in the ecology of campylobacter colonization of poultry. in a previous study, chicks with maternal ... | 2008 | 18805999 |
| comparison of disk diffusion and agar dilution methods for erythromycin, ciprofloxacin, and tetracycline susceptibility testing of campylobacter coli and for tetracycline susceptibility testing of campylobacter jejuni subsp. jejuni. | with disk diffusion, the following zone diameters are suggested to be resistant and susceptible breakpoints, respectively: for susceptibility testing of campylobacter coli, no inhibition zone and 15 mm or more for erythromycin, and 20 mm or less and 25 mm or more for ciprofloxacin, in the absence or presence of an inhibition zone around the nalidixic acid disk; and for susceptibility testing of c. coli and campylobacter jejuni, 20 mm or less and 26 mm or more for tetracycline. | 2008 | 18838597 |
| [evaluation of antigenic properties of campylobacter jejuni and campylobacter coli proteins in a western-immunoblot]. | campylobacter jejuni and campylobacter coli are the most common bacterial cause for acute diarrheal illnesses in developed countries. the aim of this study was to evaluate the antigenic properties of campylobacterjejuni and campylobacter coli proteins in western-blot assay. whole-cell components of campulobacter jejuni and campylobacter coli were separated by sodium dodecyl sulfate-polyacrylamide gel electroforesis. using this method we detected in all seven c. jejuni strains 21 peptides migrati ... | 2008 | 18819448 |
| rapid screening of salmonella enterica serovars enteritidis, hadar, heidelberg and typhimurium using a serologically-correlative allelotyping pcr targeting the o and h antigen alleles. | classical salmonella serotyping is an expensive and time consuming process that requires implementing a battery of o and h antisera to detect 2,541 different salmonella enterica serovars. for these reasons, we developed a rapid multiplex polymerase chain reaction (pcr)-based typing scheme to screen for the prevalent s. enterica serovars enteritidis, hadar, heidelberg, and typhimurium. | 2008 | 18845003 |
| multilocus sequence typing, pulsed-field gel electrophoresis, and fla short variable region typing of clonal complexes of campylobacter jejuni strains of human, bovine, and poultry origins in luxembourg. | campylobacter jejuni is the most common cause of bacterial gastroenteritis in luxembourg, with a marked seasonal peak during summer. the majority of these infections are thought to be sporadic, and the relative contribution of potential sources and reservoirs is still poorly understood. we monitored human cases from june to september 2006 (n = 124) by molecular characterization of isolates with the aim of rapidly detecting temporally related cases. in addition, isolates from poultry meat (n = 36 ... | 2008 | 18931296 |
| development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of vibrio parahaemolyticus. | vibrio parahaemolyticus is a marine seafood-borne pathogen causing gastrointestinal disorders in humans. thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are known as major virulence determinants of v. parahaemolyticus. most v. parahaemolyticus isolates from the environment do not produce tdh or trh. total v. parahaemolyticus has been used as an indicator for control of seafood contamination toward prevention of infection. detection of total v. parahaemolyticus using conventio ... | 2008 | 18823567 |
| influence of long time storage in mineral water on rna stability of pseudomonas aeruginosa and escherichia coli after heat inactivation. | research of rna viability markers was previously studied for many bacterial species. few and different targets of each species have been checked and motley results can be found in literature. no research has been done about pseudomonas aeruginosa in this way. | 2008 | 18941615 |
| antimicrobial resistance of campylobacter coli isolates from swine. | eighty-three swine isolates of campylobacter coli were tested for mechanisms underlying resistance to nalidixic acid, ciprofloxacin, erythromycin and tetracycline. four isolates harbored class 1 integrons but none carried class 2 and 3 integrons. most of the tetracycline-resistant isolates (97%) possessed tet(o). a thr-86-ile substitution in gyra and an a-2230-g mutation in 23s rrna were the main resistance mechanisms for quinolones and erythromycin, respectively. | 2008 | 18945505 |
| longitudinal study of prevalence of campylobacter jejuni and campylobacter coli from turkeys and swine grown in close proximity. | eastern north carolina is a major contributor to both turkey and swine production in the united states. in this region, turkeys and swine are frequently grown in close proximity and by common growers. to further characterize colonization of turkeys and swine with campylobacter in such a setting, we investigated the prevalence of thermophilic campylobacters in eight paired operations involving turkey farms in close proximity to finishing swine farms. all 15 surveyed flocks and 15 herds were campy ... | 2008 | 18810862 |
| a large waterborne outbreak of campylobacteriosis in norway: the need to focus on distribution system safety. | on 7 may 2007 the medical officer in røros (population 5600) reported 15 patients with gastroenteritis. three days later he estimated hundreds being ill. untreated tap water from a groundwater source was suspected as the vehicle and chlorination was started 11 may. campylobacter was isolated from patients' stool samples. we conducted an investigation to identify the source and describe the extent of the outbreak. | 2008 | 18816387 |
| recombination-based in vivo expression technology identifies helicobacter pylori genes important for host colonization. | here we undertook to identify colonization and gastric disease-promoting factors of the human gastric pathogen helicobacter pylori as genes that were induced in response to the stomach environment. using recombination-based in vivo expression technology (rivet), we identified six promoters induced in the host compared to laboratory conditions. three of these promoters, designated pivi10, pivi66, and pivi77, regulate genes that h. pylori may use to interact with other microbes or the host. pivi10 ... | 2008 | 18794279 |
| monoclonal antibody binding to the major outer membrane protein of campylobacter coli. | campylobacter species are major enteric pathogens causing diarrhea illness in humans and animals. immunological tests are needed for accurate and rapid identification of c. coli, in conjunction with the use of standard biochemical tests. we initiated the creation of monoclonal antibodies (mabs) using whole c. coli cells as antigen. four positive clones were identified, namely mab2g6, mab3b9, mab4a10 and mab5b9. dot-blot assay and elisa revealed that only mab2g6 did not cross react with c. jejuni ... | 2008 | 18796306 |
| prevalence of thermotolerant campylobacter in pheasants (phasianus colchicus). | the present study was undertaken with the aim to evaluate the prevalence of thermotolerant campylobacter spp. in living pheasants in italy. to achieve this goal, a total of 240 living pheasants, equally shared between female and male birds, were examined. thermotolerant campylobacter spp. was isolated in 104 out of 204 (43.3%) living pheasants analysed. campylobacter coli (100%) and campylobacter jejuni (13.5%) were identified by polymerase chain reaction. adult pheasants showed a significantly ... | 2008 | 18798025 |
| characterization of two campylobacter jejuni strains for use in volunteer experimental-infection studies. | the development of vaccines against campylobacter jejuni would be facilitated by the ability to perform phase ii challenge studies. however, molecular mimicry of the lipooligosaccharide (los) of most c. jejuni strains with human gangliosides presents safety concerns about the development of guillain-barré syndrome. clinical isolates of c. jejuni that appeared to lack genes for the synthesis of ganglioside mimics were identified by dna probe analyses. two clinical isolates from southeast asia (st ... | 2008 | 18809665 |
| lack of response of int-407 cells to the presence of non-culturable campylobacter jejuni. | many contradictory articles on the infectivity of non-culturable campylobacter jejuni can be found. we studied the effect of non-culturable c. jejuni in an in vitro assay. to prevent the potential effect of a few culturable bacteria in the non-culturable suspension, int-407 cells, which mimic the outer cell layer in the small intestines, were exposed to culturable c. jejuni suspensions with or without non-culturable c. jejuni. the number of bacteria adhering to and/or invading int-407 cells and ... | 2008 | 18081950 |
| characterization of two putative cytochrome c peroxidases of campylobacter jejuni involved in promoting commensal colonization of poultry. | campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans throughout the world, but infection of animals, especially poultry, results in a commensal colonization of the intestines. we previously found that a mutant lacking doca, which encodes a putative cytochrome c peroxidase (ccp), demonstrates up to a 10(5)-fold reduction in colonization of the chick cecum compared to wild-type c. jejuni strain 81-176. predictions from genomic sequences identified cjj0382 as a second locu ... | 2008 | 18086814 |