Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| resistance to antimicrobial agents used for animal therapy in pathogenic-, zoonotic- and indicator bacteria isolated from different food animals in denmark: a baseline study for the danish integrated antimicrobial resistance monitoring programme (danmap). | this study describes the establishment and first results of a continuous surveillance system of antimicrobial resistance among bacteria isolated from pigs, cattle and broilers in denmark. the three categories of bacteria tested were: 1) indicator bacteria (escherichia coli, enterococcus faecalis, enterococcus faecium), 2) zoonotic bacteria (campylobacter coli/jejuni, salmonella enterica, yersinia enterocolitica), and 3) animal pathogens (e. coli, staphylococcus aureus, coagulase-negative staphyl ... | 1998 | 9744762 |
| in vitro genotypic variation of campylobacter coli documented by pulsed-field gel electrophoretic dna profiling: implications for epidemiological studies. | six isolates of campylobacter coli from different pig herds were subcultured up to 50 times over a 6-month period and dna samples suitable for pulsed-field gel electrophoretic (pfge) profiling prepared at regular (1, 20, 40 and 50 passages) intervals. in 5/6 strains, changes in the banding patterns of sma1, sal1 and/or bamh1 digests were observed. in one such strain the differences were considered artifactual. however, significant alterations in pfge profiles between subcultures of four strains ... | 1998 | 9742707 |
| metronidazole resistance and microaerophily in campylobacter species. | metronidazole is active against most anaerobic organisms and is also used in the treatment of the microaerophilic bacterium helicobacter pylori. resistance to metronidazole is uncommon in most anaerobic organisms, but it is increasingly prevalent in h. pylori. previously we have suggested that metronidazole resistance in h. pylori is inherent in the microaerophilic nature of the organism and therefore would be present in other microaerophiles such as campylobacter. short periods of anaerobiosis ... | 1998 | 9732442 |
| the lipopolysaccharide biosynthesis locus of campylobacter jejuni 81116. | most campylobacter jejuni strains express lipo-oligosaccharides. some strains also express lipopolysaccharides (lps), with o-antigen-like carbohydrate repeats. c. jejuni 81116 expresses an lps containing both lipo-oligosaccharides and o-antigen-like repeats, but nothing is known about the structure or sugar composition of these lps species. a cosmid library of the genome of c. jejuni 81116 was constructed and probed with campylobacter hyoilei genes involved in lps synthesis. five cosmids hybridi ... | 1998 | 9720026 |
| mammalian cytidine 5'-monophosphate n-acetylneuraminic acid synthetase: a nuclear protein with evolutionarily conserved structural motifs. | sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. the pattern of cell surface sialylation is species- and tissue-specific, is highly regulated during embryonic development, and changes with stages of differentiation. a prerequisite for the synthesis of sialylated glycoconjugates is the activated sugar-nucleotide cytidine 5'-monophosphate n-acetylneuraminic acid (cmp-neu5ac), which provides a substrate for golgi sialylt ... | 1998 | 9689047 |
| antimicrobial resistance of clinical strains of campylobacter jejuni subsp. jejuni isolated from 1985 to 1997 in quebec, canada. | the antimicrobial resistance of 158 campylobacter jejuni strains isolated from humans in quebec, canada, from 1995 to 1997 was compared to the resistance of 47 and 86 strains of c. jejuni isolated in 1985 and 1986 and in 1992 and 1993, respectively. of the 291 c. jejuni strains tested, no strain was resistant to erythromycin. compared to the c. jejuni strains isolated in 1985 and 1986, the c. jejuni strains isolated in 1992 and 1993 were more resistant to tetracycline (40.7 versus 19.1%, respect ... | 1998 | 9687415 |
| incidence and clinical manifestations of campylobacter enteritis in central taiwan. | campylobacter species are recognized as a major cause of acute gastroenteritis in humans throughout the world and are increasingly associated with the development of guillain-barré syndrome. the age distribution of persons and the seasonal patterns of campylobacter infection are different in many developed and developing countries. the present study reports the investigation of the clinical and epidemiologic features of campylobacter infections in central taiwan. | 1998 | 9684510 |
| phospholipase a of yersinia enterocolitica contributes to pathogenesis in a mouse model. | some isolates of yersinia enterocolitica exhibit phospholipase activity, which has been linked to lecithin-dependent hemolysis (m. tsubokura, k. otsoki, i. shimohira, and h. yamamoto, infect. immun. 25:939-942, 1979). a gene encoding y. enterocolitica phospholipase was identified, and analysis of the nucleotide sequence revealed two tandemly transcribed open reading frames. the first, ypla, has 74% identity and 85% similarity to the phospholipase a found in serratia liquefaciens. though the othe ... | 1998 | 9673284 |
| lipopolysaccharides from campylobacter jejuni o:41 strains associated with guillain-barré syndrome exhibit mimicry of gm1 ganglioside. | three campylobacter jejuni, biotype 2, serotype o:41 strains that were isolated from patients who developed guillain-barré syndrome (gbs) and one c. jejuni isolate from a patient who developed enteritis only were examined. the aim of the study was to determine the structure of the core oligosaccharide (os) of the lipopolysaccharide (lps) of c. jejuni serotype o:41, a serotype rarely associated with the development of gbs, and to determine if the lps shares similar epitopes with any of the major ... | 1998 | 9673245 |
| associations between heat-stable (o) and heat-labile (hl) serogroup antigens of campylobacter jejuni: evidence for interstrain relationships within three o/hl serovars. | a comparative examination of the heat-stable (o) and heat-labile (hl) serogrouping results for 9,024 sporadic human isolates of campylobacter jejuni revealed conserved associations between specific o and hl antigens (o/hl serovars). forty-nine percent of the isolates which grouped for both o and hl antigens belonged to one of three serovars: o 4 complex/hl 1 (17.9%), o 1/hl 2 (16.8%), or o 50/hl 7 (14.5%). other common serovars were o 2/hl 4 (8.3%), o 6/hl 6 (8.1%), o 53/hl 11 (4.5%), o 19/hl 17 ... | 1998 | 9665996 |
| etiological study of diarrheal patients in vientiane, lao people's democratic republic. | the etiological agents of diarrhea in vientiane, lao people's democratic republic (lao pdr), were studied in the period from october 1996 to august 1997. a total of 880 patients with diarrhea visiting medical facilities were examined for shigella, salmonella, diarrheagenic escherichia coli, vibrio, aeromonas, campylobacter, and rotavirus. shigella spp., heat-stable enterotoxin (st)-producing e. coli, and serogroup-based enteropathogenic e. coli were found to be the main organisms causing diarrhe ... | 1998 | 9665990 |
| campylobacter species and guillain-barré syndrome. | since the eradication of polio in most parts of the world, guillain-barré syndrome (gbs) has become the most common cause of acute flaccid paralysis. gbs is an autoimmune disorder of the peripheral nervous system characterized by weakness, usually symmetrical, evolving over a period of several days or more. since laboratories began to isolate campylobacter species from stool specimens some 20 years ago, there have been many reports of gbs following campylobacter infection. only during the past f ... | 1998 | 9665983 |
| campylobacter upsaliensis: waiting in the wings. | despite strong epidemiological evidence supporting an important role for campylobacter upsaliensis as a human enteropathogen, it remains relatively unknown in the realm of clinical microbiology. clinical studies indicate that infection with this organism usually is associated with benign self-limiting diarrhea. however, more serious illnesses, including spontaneous abortion and hemolytic-uremic syndrome, recently have been associated with human infections. understanding of the virulence properti ... | 1998 | 9665977 |
| antimicrobial susceptibility testing of 59 strains of campylobacter fetus subsp. fetus. | the susceptibilities of 59 campylobacter fetus subsp. fetus isolates to eight antibiotics were studied by the agar dilution, e-test, and disk diffusion methods. none of the isolates were beta-lactamase producers. all were susceptible to ampicillin, gentamicin, imipenem, and meropenem as determined by the three methods, with mics at which 90% of the isolates are inhibited (mic90s) (determined by agar dilution) of 2, 1, < or = 0.06, and 0.12 microgram/ml, respectively. twenty-seven percent of the ... | 1998 | 9661033 |
| the central, surface-exposed region of the flagellar hook protein flge of campylobacter jejuni shows hypervariability among strains. | in a previous study, we observed that monoclonal antibodies raised against the hook protein flge of campylobacter jejuni lio 36, isolate 5226, bound exclusively to this strain. the aim of this study was to elucidate the molecular basis for these binding specificities. the hook protein-encoding gene flge of c. jejuni was cloned in escherichia coli and sequenced. the flge genes of four additional c. jejuni strains were amplified by pcr and also sequenced. comparison of the deduced amino acid seque ... | 1998 | 9658019 |
| microtiter assay for detecting campylobacter spp. and helicobacter pylori with surface gangliosides which bind cholera toxin. | campylobacter jejuni with gm1 ganglioside in the core of its lipopolysaccharide has been associated with guillain-barré syndrome. since this epitope may be of considerable pathophysiologic importance and since this ganglioside binds cholera toxin, a rapid screening assay to detect bacteria that bind cholera toxin as an indication of gm1 on their surfaces was developed. in the assay, bacterial lawns were grown on agar plates, harvested with phosphate-buffered saline, boiled, and incubated with a ... | 1998 | 9650959 |
| epidemiological typing of campylobacter isolates from meat processing plants by pulsed-field gel electrophoresis, fatty acid profile typing, serotyping, and biotyping. | campylobacter spp. are a leading cause of bacterial gastroenteritis. foods of animal origin, particularly under-cooked poultry, are common sources of campylobacter species associated with disease in humans. a collection of 110 campylobacter jejuni and 31 c. coli human and environmental isolates from different ontario, canada, abattoirs were analyzed by pulsed-field gel electrophoresis, fatty acid profile typing, and biotyping. previously collected serotyping data for the same isolates were also ... | 1998 | 9647797 |
| isolation and molecular analysis of colonising and non-colonising strains of campylobacter jejuni and campylobacter coli following experimental infection of young chickens. | fourteen-day-old chickens were inoculated with selected campylobacter coli and c. jejuni strains. c. jejuni strains were of two subgroups based on a polymorphism detected using a dna probe and represented the profiles typical for the majority of strains of either chicken or human origin. all c. coli strains previously isolated from humans colonised chickens, whereas from 4/7 c. jejuni strains of human origin, failed to colonise. of 12 campylobacter strains of chicken origin, 10 established a per ... | 1998 | 9646454 |
| heterogeneity in levels of vacuolating cytotoxin gene (vaca) transcription among helicobacter pylori strains. | broth culture supernatants from tox+ helicobacter pylori strains induce vacuolation of hela cells in vitro and contain vaca in concentrations that are higher than those found in supernatants from tox- h. pylori strains. to investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cytotoxin gene (vaca) in eight tox+ strains (each with a type s1/m1 vaca genotype) and nine tox- strains (each with a type s2/m2 vaca genotype). most of the tox+ and tox- strains tested ... | 1998 | 9632570 |
| an outbreak of infectious hepatitis in commercially reared ostriches associated with campylobacter coli and campylobacter jejuni. | a disease causing high morbidity and mortality was observed in young ostriches from six properties in southeast queensland, australia. the disease affected birds from 2-8 weeks of age and was characterised clinically by bright-green urates and pathologically by severe necrotic hepatitis. the liver lesions resembled those of vibrionic hepatitis in other avian species. campylobacter coli was isolated from the livers of affected ostriches from five of the six properties. campylobacter jejuni subsp. ... | 1998 | 9631530 |
| cloning and comparison of flic genes and identification of glycosylation in the flagellin of pseudomonas aeruginosa a-type strains. | pseudomonas aeruginosa a-type strains produce flagellin proteins which vary in molecular weight between strains. to compare the properties of a-type flagellins, the flagellin genes of several pseudomonas aeruginosa a-type strains, as determined by interaction with specific anti-a monoclonal antibody, were cloned and sequenced. pcr amplification of the a-type flagellin gene fragments from five strains each yielded a 1.02-kb product, indicating that the gene size is not likely to be responsible fo ... | 1998 | 9620973 |
| typing of human campylobacter jejuni isolates in finland by pulsed-field gel electrophoresis. | a total of 69 pulsed-field gel electrophoresis (pfge) types were identified among 176 campylobacter jejuni isolates from finnish patients. in two geographic areas studied, five predominant pfge types comprised over 40% of the isolates. one-third of the isolates had unique pfge types. in small outbreaks, identical pfge patterns were demonstrated, indicating a common source of infection. | 1998 | 9620423 |
| short-sequence dna repeats in prokaryotic genomes. | short-sequence dna repeat (ssr) loci can be identified in all eukaryotic and many prokaryotic genomes. these loci harbor short or long stretches of repeated nucleotide sequence motifs. dna sequence motifs in a single locus can be identical and/or heterogeneous. ssrs are encountered in many different branches of the prokaryote kingdom. they are found in genes encoding products as diverse as microbial surface components recognizing adhesive matrix molecules and specific bacterial virulence factors ... | 1998 | 9618442 |
| characterization of chemotactic responses and flagella of hyphomicrobium strain w1-1b. | motile swarmer cells of hyphomicrobium strain w1-1b displayed positive chemotactic responses toward methylamine, dimethylamine, and trimethylamine but did not display significant chemotactic responses towards methanol and arginine. electron micrographs of negatively stained intact flagellar filaments indicated a novel striated surface pattern. the flagella were composed of two proteins of 39 and 41 kda. neither protein was a glycoprotein as determined by schiff's staining and by enzyme immunoass ... | 1998 | 9603896 |
| campylobacter jejuni cytolethal distending toxin causes a g2-phase cell cycle block. | cytolethal distending toxin (cdt) from the diarrheagenic bacterium campylobacter jejuni was shown to cause a rapid and specific cell cycle arrest in hela and caco-2 cells. within 24 h of treatment, cdt caused hela cells to arrest with a 4n dna content, indicative of cells in g2 or early m phase. immunofluorescence studies indicated that the arrested cells had not entered m phase, since no evidence of tubulin reorganization or chromatin condensation was visible. cdt treatment was also shown to ca ... | 1998 | 9573072 |
| helicobacter pylori glutamine synthetase lacks features associated with transcriptional and posttranslational regulation. | helicobacter pylori urease, produced in abundance, is indispensable for the survival of h. pylori in animal hosts. urea is hydrolyzed by the enzyme, resulting in the liberation of excess ammonia, some of which neutralizes gastric acid. the remaining ammonia is assimilated into protein by glutamine synthetase (ec 6.3.1.2), which catalyzes the reaction: nh3 + glutamate + atp-->glutamine + adp + pi. we hypothesized that glutamine synthetase plays an unusually critical role in nitrogen assimilation ... | 1998 | 9573059 |
| evidence of genomic instability in campylobacter jejuni isolated from poultry. | poultry isolates of campylobacter jejuni derived from a survey of meat processing batches were genotyped by pulsed-field gel electrophoresis (pfge) of chromosomal dna to establish the clonal relationships between single-colony isolates. in the majority of batches studied, one or two genotype patterns predominated. however, in one batch (batch a), 21 single-colony isolates gave 14 different pfge genotypes. the banding patterns obtained with smai were sufficiently different to distinguish between ... | 1998 | 9572956 |
| a rapid pcr-based dna test for enterotoxic bacillus cereus. | the occurrence of dna sequences encoding the hemolysin hbla complex and bacillus cereus enterotoxin bcet, which have recently been confirmed as enterotoxins, was studied in bacillus spp. to amplify these dna sequences, pcr primer systems for the b component of hbla and for bcet dna sequences were developed. the results from the amplification of hbla sequences correlated well with results obtained with the b. cereus enterotoxin (diarrheal type) test kit (rpla kit), but not with the results of the ... | 1998 | 9572928 |
| a cyanobacterium lacking iron superoxide dismutase is sensitized to oxidative stress induced with methyl viologen but is not sensitized to oxidative stress induced with norflurazon | a strain of synechococcus sp. strain pcc 7942 with no functional fe superoxide dismutase (sod), designated sodb-, was characterized by its growth rate, photosynthetic pigments, and cyclic photosynthetic electron transport activity when treated with methyl viologen or norflurazon (nf). in their unstressed conditions, both the sodb- and wild-type strains had similar chlorophyll and carotenoid contents and catalase activity, but the wild type had a faster growth rate and higher cyclic electron tran ... | 1998 | 9536078 |
| ribosomal rna gene restriction fragment diversity amongst penner serotypes of campylobacter jejuni and campylobacter coli. | diversity based on ribosomal rna gene-restriction endonuclease digest patterns was detected amongst forty-seven strains of campylobacter made up of 38 strains of campylobacter jejuni and 9 strains of campylobacter coli. restriction digests of chromosomal dna prepared by treating with hae iii were probed with an oligonucleotide specific for campylobacter 16s ribosomal rna genes. seventeen distinct hybridization patterns, each indicating the presence of 2-4 copies of the 16s rrna gene are encoded ... | 1998 | 9528123 |
| enrichment medium for isolation of campylobacter jejuni-campylobacter coli. | a kaseini-broth enrichment medium (kbem) for the isolation improvement of campylobacter jejuni-campylobacter coli from stool samples is described. isolation of campylobacters from stool specimens by direct inoculation onto solid selective medium was compared with isolation after twenty-four hours enrichment at 37 degrees c in kbem, followed by subculture onto the same solid selective medium. of 156 examined stool samples from diarrhoeal children positive results were obtained from 17 patients al ... | 1995 | 9524671 |
| transcription of ppk from acinetobacter sp. strain adp1, encoding a putative polyphosphate kinase, is induced by phosphate starvation. | polyphosphate kinase (ppk) catalyzes the formation of polyphosphate from atp. we cloned the ppk gene (2,073 bp) from acinetobacter sp. strain adp1; this gene encodes a putative polypeptide of 78.6 kda with extensive homology to polyphosphate kinase from escherichia coli and other bacteria. chromosomal disruption of ppk by inserting a transcriptionally fused lacz does not affect growth under conditions of phosphate limitation or excess. beta-galactosidase activity expressed from the single-copy p ... | 1998 | 9501429 |
| helicobacter pylori porcdab and oordabc genes encode distinct pyruvate:flavodoxin and 2-oxoglutarate:acceptor oxidoreductases which mediate electron transport to nadp. | helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. previous studies (n. j. hughes, p. a. chalk, c. l. clayton, and d. j. kelly, j. bacteriol. 177:3953-3959, 1995) have indicated that the major routes for the generation of acetyl coenzyme a (acetyl-coa) and succinyl-coa are via pyruvate:flavodoxin oxidoreductase (por) and 2-oxoglutarate:acceptor oxidoreductase (oor), respectively. th ... | 1998 | 9495749 |
| variation in flagellin genes and proteins of burkholderia cepacia. | the majority of isolates of burkholderia cepacia, an important opportunistic pathogen associated with cystic fibrosis, can be classified into two types on the basis of flagellin protein size. electron microscopic analysis indicates that the flagella of strains with the larger flagellin type (type i) are wider in diameter. flagellin genes representative of both types were cloned and sequenced to design oligonucleotide primers for pcr amplification of the central variable domain of b. cepacia flag ... | 1998 | 9495748 |
| the pseudomonas aeruginosa flagellar cap protein, flid, is responsible for mucin adhesion. | mucin-specific adhesion of pseudomonas aeruginosa plays an important role in the initial colonization of this organism in the airways of cystic fibrosis patients. we report here that the flagellar cap protein, flid, participates in this adhesion process. a polar chromosomal insertional mutation in the p. aeruginosa flid gene made this organism nonadhesive to mucin in an in vitro mucin adhesion assay. the adhesive phenotype was restored by providing the flid gene alone on a multicopy plasmid, sug ... | 1998 | 9488388 |
| mutation in the peb1a locus of campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. | campylobacter jejuni is one of the leading causes of bacterial diarrhea throughout the world. we previously found that peb1 is a homolog of cluster 3 binding proteins of bacterial abc transporters and that a c. jejuni adhesin, cell-binding factor 1 (cbf1), if not identical to, contains peb1. a single protein migrating at approximately 27 to 28 kda was recognized by anti-cbf1 and anti-peb1. to determine the role that the operon encoding peb1 plays in c. jejuni adherence, peb1a, the gene encoding ... | 1998 | 9488379 |
| trna(arg) (fimu) and expression of sef14 and sef21 in salmonella enteritidis. | a tn10 insertion affecting sef14 fimbrial synthesis in salmonella enteritidis was located 13 bp upstream of a gene designated fimu. the 77-bp dna sequence of fimu from s. enteritidis was identical to that of fimu encoding trna(arg) (ucu) from salmonella typhimurium and 96% identical to that of the escherichia coli argu homolog. furthermore, the open reading frame adjacent to and overlapping the 3' end of fimu was similar to the prophage dlp12 integrase gene. the fimu-encoded transcript comigrate ... | 1998 | 9473037 |
| serotyping scheme for campylobacter jejuni and campylobacter coli based on direct agglutination of heat-stable antigens. | campylobacter is now the most frequently reported cause of gastrointestinal disease in england and wales, yet few isolates are characterized beyond the genus level. the majority of isolates are campylobacter jejuni (90%), with most of the remainder being campylobacter coli. we describe an adaptation of the penner serotyping scheme in which passive hemagglutination has been replaced by detection of heat-stable antigens by direct bacterial agglutination; absorbed antisera are used where appropriat ... | 1998 | 9466737 |
| extended survival and persistence of campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and -rrna staining. | in water microcosm experiments, the survival times of campylobacter isolates differed by up to twofold, as determined by culturing; this difference increased to fourfold when particular combinations of temperature and oxygenation were used. the mean survival times were much longer at 4 and 10 degrees c (202 and 176 h, respectively) than at 22 and 37 degrees c (43 and 22 h, respectively). the influence of anaerobiosis on survival time was less dramatic and differed considerably between isolates. ... | 1998 | 9464415 |
| effect of low temperatures on growth, structure, and metabolism of campylobacter coli sp10. | the effect of low temperatures on the survival, structure, and metabolism of campylobacter coli sp10, a virulent strain, was investigated. c. coli became nonculturable rapidly at 20 and 10 degree c and slightly later at 4 degrees c. incubation in a microaerobic atmosphere improved survival, but after day 8, campylobacters were detectable by direct-count procedures only. the increase in the number of coccoid cells was most pronounced at 37 degrees c but also was noticeable at 20 and 10 degrees c. ... | 1998 | 9464397 |
| identification of campylobacter jejuni promoter sequences. | a promoterless lacz shuttle vector, which allowed screening of promoters by beta-galactosidase activity in campylobacter jejuni and escherichia coli, was developed. chromosomal dna fragments from c. jejuni were cloned into this vector; 125 of 1,824 clones displayed promoter activity in c. jejuni. eleven clones with strong promoter activity in c. jejuni were further characterized. their nucleotide sequences were determined, and the transcriptional start sites of the putative promoters in c. jejun ... | 1998 | 9457862 |
| comparison of a novel microaerobic system with three other gas-generating systems for the recovery of campylobacter species from human faecal samples. | three commercial gas-generating systems--campygen (oxoid, uk), oxoid br56 (oxoid, uk), and campypak plus (becton dickinson, usa)--and the evacuation replacement technique were compared for the recovery of campylobacter spp. from 500 human faecal samples collected from patients with gastroenteritis. four hundred fifty faecal samples were tested upon receipt in the laboratory. fifty faecal samples that had been previously found to be positive for campylobacter spp. were tested retrospectively; the ... | 1997 | 9447907 |
| differentiation of campylobacter isolates on the basis of sensitivity to boiling in water as measured by pcr-detectable dna. | differential sensitivity for the release of pcr-detectable genomic dna upon boiling in water is reported for 45 campylobacter jejuni and campylobacter coli strains isolated in egypt. all of the strains released pcr-detectable dna when treated with proteinase k and sodium dodecyl sulfate. when dna was extracted from these strains by boiling in water, nine (20%) of the strains were pcr negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes. | 1998 | 9435091 |
| analysis of distribution of campylobacter jejuni and campylobacter coli in broilers by using restriction fragment length polymorphism of flagellin gene. | the incidence of campylobacter jejuni and campylobacter coli in broiler farms was 33.9% (19/56). c. jejuni-positive flocks accounted for 20.0% (17/85) and c. coli-positive ones was 4.7% (4/85). there were 14 patterns (fla type) of restriction fragment length polymorphism (rflp) of flagellin a gene among these 22 strains of c. jejuni and c. coli including the standard strain c. jejuni atcc 33560. different fla types of campylobacter were isolated from broilers in different growing cycles on the s ... | 1997 | 9409516 |
| expression of campylobacter hyoilei lipo-oligosaccharide (los) antigens in escherichia coli. | campylobacter spp. are well recognized as primary pathogens in animals and in people. to isolate and define the genetic regions encoding major surface antigens of campylobacter hyoilei, genomic dna of the type strain of the species, rmit-32a, was cloned into a cosmid vector, pla2917, in escherichia coli and the resulting genomic library was screened using antiserum raised to the parent c. hyoilei strain. six cosmid clones were found to express a series of immunoreactive bands in the 15-25 kda ra ... | 1997 | 9387226 |
| [the sat4 streptothricin acetyltransferase gene of campylobacter coli: its distribution in the environment and use as epidemiological marker]. | the main of this study is to give evidence about the spread of streptothricin resistance within campylobacters which were isolated from animals, men and environmental sources. streptothricin resistant campylobacters were isolated over a five years period, when the use of streptothricin for ergotropic purposes was not allowed, from slurry of swine, slurry of cattle, waste water of a goose farm and waste water of a communal sewage treatment plant. the streptothricin resistance was found to be conn ... | 1996 | 9376053 |
| nucleotide sequence of the staphylococcus aureus transposon, tn5405, carrying aminoglycosides resistance genes. | tn5405 is a 12 kb staphylococcal composite transposon delimited by two inverted copies of the insertion sequence is1182. this transposon carries two aminoglycosides resistance genes, apha-3 and aade, an altered gene similar to sat4 from campylobacter coli be/g4, and three open reading frames of unknown functions. | 1997 | 9373952 |
| specific detection and confirmation of campylobacter jejuni by dna hybridization and pcr. | conventional detection and confirmation methods for campylobacter jejuni are lengthy and tedious. a rapid hybridization protocol in which a 1,475-bp chromogen-labelled dna probe (pdt1720) and campylobacter strains filtered and grown on 0.22-micron-pore-size hydrophobic grid membrane filters (hgmfs) are used was developed. among the environmental and clinical isolates of c. jejuni, campylobacter coli, campylobacter jejuni subsp. doylei, campylobacter lari, and arcobacter nitrofigilis and a panel ... | 1997 | 9361442 |
| campylobacter hyoilei alderton et al. 1995 and campylobacter coli véron and chatelain 1973 are subjective synonyms. | the taxonomic affiliation of campylobacter hyoilei was reevaluated by examining a variety of phenotypic and genotypic criteria. whole-cell protein electrophoresis and a comparison of 66 phenotypic characters revealed that reference strains of c. hyoilei were indistinguishable from campylobacter coli strains. these data were confirmed by a dna-dna hybridization level of 67% between the type strains of the two species. several species-specific assays based on pcr amplification or probe hybridizati ... | 1997 | 9336905 |
| antimicrobial susceptibility patterns of thermophilic campylobacter spp. from humans, pigs, cattle, and broilers in denmark. | the mics of 16 antimicrobial agents were determined for 202 campylobacter jejuni isolates, 123 campylobacter coli isolates, and 6 campylobacter lari isolates from humans and food animals in denmark. the c. jejuni isolates originated from humans (75), broilers (95), cattle (29), and pigs (3); the c. coli isolates originated from humans (7), broilers (17), and pigs (99); and the c. lari isolates originated from broilers (5) and cattle (1). all isolates were susceptible to apramycin, neomycin, and ... | 1997 | 9333055 |
| pcr detection, identification to species level, and fingerprinting of campylobacter jejuni and campylobacter coli direct from diarrheic samples. | three sets of primers were designed for pcr detection and differentiation of campylobacter jejuni and campylobacter coli. the first pcr assay was designed to coidentify c. jejuni and c. coli based on their 16s rrna gene sequences. the second pcr assay, based on the hippuricase gene sequence, identified all tested reference strains of c. jejuni and also strains of that species which lack detectable hippuricase activity. the third pcr assay, based on the sequence of a cloned (putative) aspartokina ... | 1997 | 9316909 |
| evaluation of antibodies reactive with campylobacter jejuni in egyptian diarrhea patients. | serum and stool samples were collected from 128 individuals: 96 diarrhea patients and 32 apparently healthy controls. stool specimens were cultured for enteric bacterial pathogens, while sera were screened by enzyme-linked immunosorbent assay for campylobacter jejuni-reactive antibodies. of 28 diarrhea patients who demonstrated c. jejuni-reactive antibodies (titers, > 100), 14 were culture positive for this organism. the 32 healthy controls showed significantly lower antibody titers (p < 0.05) w ... | 1997 | 9302201 |
| norfloxacin resistance in campylobacter jejuni and campylobacter coli isolates from swedish patients. | this study focused on the frequency of campylobacter jejuni and campylobacter coli strains resistant to norfloxacin. included were 1659 consecutive stool specimens isolated between 1992 and 1995, from as many swedish patients with diarrhoea. the patients were divided with regard to place of infection and age. all strains were tested for susceptibility to norfloxacin by means of disc diffusion test on blood-agar plates. norfloxacin-resistant strains (n = 310) were furthermore tested for resistanc ... | 1997 | 9301992 |
| [serodiagnosis of helicobacter pylori infections by detection of immunoglobulin g antibodies with an immunoblot technique]. | serological testing has recently been proposed as an aid in diagnosis of h. pylori infections. in this study, an immunoblot method has been evaluated to diagnose h. pylori infection serologically by comparing 104 serum specimens from patients with a positive gram stain and/or culture result and a positive urease test on biopsy material, as well as 30 serum specimens from patients with negative urease test, and negative microscopy and culture results. thirteen bands could be identified by immunob ... | 1996 | 9275637 |
| invasive and enterotoxic properties in campylobacter jejuni and campylobacter coli strains isolated from humans and animals. | invasive properties of 15 strains of thermotolerant campylobacter (12 c. jejuni and 3 c. coli) were studied using hela cells cultures. in four of them (3 c. jejuni and 1 c. coli), randomly selected, intestinal perfusion experiments were conducted in order to asses enterotoxigenicity. all strains were able to invade hela cells. the number of invaded hela cells ranged from 3 to 46%. in addition to their invasive properties, the 4 strains used in perfusion experiments were able to induce either a n ... | 1995 | 9251750 |
| the flge gene of campylobacter coli is under the control of the alternative sigma factor sigma54. | the flge gene encoding the flagellar hook protein of campylobacter coli vc167-t1 was cloned by immunoscreening of a genomic library constructed in lambdazap express. the flge dna sequence was 2,553 bp in length and encoded a protein with a deduced molecular mass of 90,639 da. the sequence had significant homology to the 5' and 3' sequences of the flge genes of helicobacter pylori, treponema phagedenis, and salmonella typhimurium. primer extension analysis indicated that the vc167 flge gene is co ... | 1997 | 9244248 |
| differentiation of campylobacter jejuni and campylobacter coli by polymerase chain reaction. | a multiplex pcr assay was developed using two primer sets for the identification and differentiation of campylobacter coli and campylobacter jejuni. primer set i amplifies a 460-bp fragment present in c. coli and c. jejuni. set ii amplifies a 160-bp target unique to c. jejuni. when the assay was performed on reference strains, amplification of c. coli yielded only the 460-bp fragment. amplification of c. jejuni generated both the 160- and 460-bp fragments. campylobacter field strains (n = 85) is ... | 1997 | 9232618 |
| molecular discrimination between campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis by polymerase chain reaction based on a novel putative gtpase gene. | polymerase chain reaction (pcr) mediated dna fingerprinting has resulted in the identification of a novel campylobacter jejuni gene, encoding a gtpase protein. the gene, consisting of 383 amino acids contained semi-conserved gtp-binding sites (designated g-1 to g-4), that are characteristic for members of the gtpase protein superfamily. remarkably, this gene from c. jejuni appears to encode a member of a novel family of gtp-binding proteins, containing two separate putative gtp-binding domains, ... | 1997 | 9232616 |
| indwelling device-related bacteremia caused by serum-susceptible campylobacter coli. | two isolates of serum-susceptible campylobacter coli were recovered in a 7-day interval from blood from a patient with hepatocellular carcinoma and liver cirrhosis whose peritoneal-caval (denver's) shunt malfunctioned. identical random amplified polymorphic dna fingerprints, cellular fatty acid chromatograms, and antibiograms of the two isolates indicate that c. coli has the ability to cause catheter-related bacteremia following its colonization of the catheter. | 1997 | 9230411 |
| comparison of disc diffusion and agar dilution methods for antibiotic susceptibility testing of campylobacter jejuni subsp. jejuni and campylobacter coli. | the correlation between disc diffusion and agar dilution susceptibility testing of five antibiotics was studied against 145 campylobacter strains: 99 campylobacter jejuni subsp. jejuni and 46 campylobacter coli. the percentages of true results and 95% ci for disc diffusion for resistant strains were 100% (93.2-100%) for tetracycline (53 strains tested), 100% (77.2-100%) for ciprofloxacin (13 strains tested), 86.7% (62.1-96.3%) for nalidixic acid (15 strains tested), 100% (56.6-100%) for erythrom ... | 1997 | 9222038 |
| a genetic locus involved in iron utilization unique to some campylobacter strains. | two genes involved in iron utilization in campylobacter coli vc167 t1 have been characterized. the cfra gene encodes a protein with a predicted mr of 77,653 which, after processing of the leader sequence, has a predicted mr of 75,635. this protein has significant sequence identity to siderophore receptors of several bacteria, and site-specific mutants defective in cfra do not synthesize one of two major iron-repressible outer membrane proteins. an adjacent gene encodes a tonb-like protein; a mut ... | 1997 | 9190817 |
| enterotoxigenic capacity of campylobacter coli strains isolated from humans and bovines. | 1996 | 9137747 | |
| the serotype distribution of campylobacter jejuni and c. coli isolated from patients with diarrhoea and controls at tikur anbassa hospital, addis ababa, ethiopia. | sixty-eight isolates of campylobacter jejuni and c. coli isolated from patients with diarrhoea (n = 630) and controls (n = 220) at tikur anbassa hospital, addis ababa, ethiopia were serotyped on the basis of the heat-labile (hl) and the heat-stable (hs) antigens, by using 16 and 34 antisera, respectively, for the two methods. with the antisera against heat labile antigens, 89.3% of the c. jejuni and 75% of the c. coli were typable. the hl serotypes 1, 2, 3, 4, 5, 6 and 7 were the most common amo ... | 1997 | 9129584 |
| identification of the tetracycline resistance gene, tet(o), in streptococcus pneumoniae. | five isolates of streptococcus pneumoniae resistant to tetracycline but lacking tet(m) were studied. the tetracycline resistance gene, tet(o), was detected for the first time in the pneumococcus. the gene was amplified and sequenced and found to share 99% nucleotide sequence identity and 99, 99, and 98% deduced amino acid sequence identity with the tet(o) resistance genes of streptococcus mutans, campylobacter coli, and campylobacter jejuni, respectively. | 1996 | 9124862 |
| molecular characterization of plda, the structural gene for a phospholipase a from campylobacter coli, and its contribution to cell-associated hemolysis. | a gene (plda) encoding a 35.0-kda protein with significant homology to the escherichia coli outer membrane phospholipase was identified upstream of an operon encoding an enterochelin transport system in campylobacter coli. the results of this study suggest that this gene encodes an outer membrane phospholipase a in c. coli. first, expression of the plda gene product in a plda-deficient mutant of e. coli led to the restoration of phospholipase a activity. the recombinant product also partitioned ... | 1997 | 9119448 |
| campylobacter-host cell interactions. | the enteric pathogens campylobacter jejuni and campylobacter coli are a major cause of infectious diarrhoea. their ability to adhere to human epithelial cells is ubiquitous and their propensity to invade cells is also well documented and requires motility and de novo protein synthesis, as well as several host factors. the molecular basis of the interaction between campylobacters and host cells is only beginning to be elucidate. the characteristics of this interaction promise to be interesting an ... | 1997 | 9080607 |
| integration of heterologous plasmid dna into multiple sites on the genome of campylobacter coli following natural transformation. | the efficiency of homologous recombination in campylobacter coli following the introduction of dna by natural transformation was determined by using a series of nonreplicating integrative vectors containing dna fragments derived from the c. coli catalase gene. homologous recombination occurred with as little as 286 homologous bp present and was not detected when 270 bases of homology was provided. instead, when plasmids with little or no homology to the chromosome were introduced by natural tran ... | 1997 | 9045845 |
| specific identification of the enteropathogens campylobacter jejuni and campylobacter coli by using a pcr test based on the ceue gene encoding a putative virulence determinant. | a pcr method for the rapid identification and discrimination of thermophilic campylobacter jejuni and campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceue). a nucleotide sequence divergence of approximately 13% in the ceue genes of c. jejuni and c. coli facilitated the design of two species-specific pcr primer sets. the specificity of the pcr amplification reactions was confirmed by using two nonradioactively labelled species-specific intern ... | 1997 | 9041429 |
| detection of campylobacter jejuni and camp. coli in chicken faecal samples by pcr. | pcr primers were selected from the flagellin gene sequences flaa and flab of campylobacter coli to amplify dna from camp. jejuni and camp. coli. when the pcr products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of camp. jejuni and camp. coli. the assay was used to analyse 31 chicken faecal samples. full correspondence was found between the pcr assay conducted on the enriched cultures and the standard cultu ... | 1996 | 8987720 |
| a combined polymerase chain reaction and restriction endonuclease enzyme assay for discriminating between campylobacter coli and campylobacter jejuni. | a combined polymerase chain reaction and restriction endonuclease (re) enzyme assay was developed to discriminate between campylobacter coli and campylobacter jejuni. amplimers of the flaa gene obtained by pcr were digested with alui and hinfi to distinguish c. coli from c. jejuni. with alui digestion c. jejuni-specific bands were observed at 110, 140 and 160 bp and c. coli-specific bands at 293 and 147 bp. c. jejuni-specific bands of 349 and 109 bp were found by hinfi digestion but hinfi did no ... | 1996 | 8954352 |
| development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari. | contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ... | 1996 | 8914757 |
| the lipooligosaccharides of pathogenic gram-negative bacteria. | lipooligosaccharides (loss) are the major glycolipids expressed on mucosal gram-negative bacteria, including members of the genera neisseria, haemophilus, bordetella, and branhamella. they can also be expressed on some enteric bacteria such as campylobacter jejuni and campylobacter coli strains. los is analogous to the lipopolysaccharide (lps) found in other gram-negative families. loss share similar lipid a structures with an identical array of functional activities as lpss. loss lack o-antigen ... | 1996 | 8894399 |
| sub-typing of animal and human campylobacter spp. using rapd. | based on a 10-mer primer (5'-cctgttagcc-3'), a random amplified polymorphic dna (rapd) method for typing campylobacter coli isolated from pigs was developed. the method proved effective with a high discrimination and good reproducibility. in contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. the method was also successfully applied to typing campylobacter jejuni from a similar range of sources. | 1996 | 8862022 |
| hazard identification in swine slaughter with respect to foodborne bacteria. | swine slaughter is an open process with many opportunities for the contamination of the pork carcass with potentially pathogenic bacteria; however, it does not contain any point where hazards are completely eliminated. data on the prevalence of various pathogenic bacteria (aeromonas hydrophila, campylobacter coli/jejuni, listeria monocytogenes, salmonella spp., staphylococcus aureus and yersinia enterocolitica) in pigs, their growth and survival characteristics and ability to become established ... | 1996 | 8856371 |
| antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli to 12 beta-lactam agents and combinations with beta-lactamase inhibitors. | the in vitro activities of 12 beta-lactam agents against 100 thermophilic campylobacter strains were tested. beta-lactamase production was detected in 88% of all strains tested. clavulanic acid was the best inhibitor by susceptibility testing. the beta-lactams which displayed high levels of in vitro activity against campylobacter isolates were imipenem, amoxicillin-clavulanic acid, and cefepime and, to a lesser degree, amoxicillin, ampicillin, and cefotaxime. | 1996 | 8843305 |
| identification and characterization of genes required for post-translational modification of campylobacter coli vc167 flagellin. | two genes have been identified in campylobacter coli vc167 which are required for the biosynthesis of post-translational modifications on flagellin proteins. the ptma gene encodes a protein of predicted m(r) 28,486 which shows significant homology to a family of alcohol dehydrogenases from a variety of bacteria. the ptmb gene encodes a protein of predicted m(r) 26,598 with significant homology to cmp-n-acetylneuraminic acid synthetase enzymes involved in sialic acid capsular biosynthesis in neis ... | 1996 | 8825781 |
| an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis. | examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ... | 1996 | 8793884 |
| analysis of hl and o serotypes of campylobacter strains by the flagellin gene typing system. | we recently developed a molecular typing system for campylobacter jejuni and campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaa (i.nachamkin, k. bohachick, and c.m. patton, j. clin. microbiol. 31:1531-1536, 1993). we extended the typing system to 83 flagellin types (designated flaa-1,flaa-2, etc.) on the basis of analysis of 404 isolates of c. jejuni and c. coli including common serotypes isolated in the united states, a selection of less com ... | 1996 | 8789000 |
| comprehensive ribotyping scheme for heat-stable serotypes of campylobacter jejuni. | strains from diverse sources belonging to all 47 heat-stable penner serotypes of campylobacter jejuni were examined for polymorphism around the 16s rrna genes. penner serotype reference strains and a group of nonserotypeable isolates were included in the study. complete typeability was obtained; 30 distinct psti and 42 haeiii polymorphisms were found. three bands were detected in almost all strains with these enzymes, confirming that three copies of the 16s rrna gene are typical for c.jejuni. by ... | 1996 | 8788998 |
| soma, a novel gene that encodes a major outer-membrane protein of synechococcus sp. pcc 7942. | the outer membrane of a cyanobacterium (synechococcus sp. strain pcc 7942) contains only a few major proteins. a gene encoding one of them, soma, was cloned and characterized. based on the nucleotide sequence, soma was predicted to comprise 531 amino acids with a calculated molecular mass of 57,136 da. the deduced amino acid sequence of soma shares similarities with two bacterial cell-surface proteins, the s-layer protein of thermus thermophilus and the flagellin of campylobacter coli. the predi ... | 1996 | 8760925 |
| restriction fragment length polymorphism of flagellin genes of campylobacter jejuni and/or c. coli isolates from egypt. | the conservation of flagellin genes from thermophilic campylobacter spp. strains isolated in egypt was evaluated by a restriction fragment length polymorphism (rflp) assay. the flaa and flab genes were amplified from 59 independent clinical isolates and digested with ecori and psti, and the resulting patterns were compared with each other and with previously described rflp groups. the results indicate that the isolates fell into 14 groups for flaa and 11 groups for flab, 9 of which have been des ... | 1996 | 8727905 |
| ciprofloxacin resistant campylobacter spp. in humans: an epidemiological and laboratory study. | from the end of april 1991 until the end of 1991, 2209 isolates of campylobacter spp. have been collected in plymouth phl of which 91 (4.1%) were resistant to ciprofloxacin. none of the 91 patients involved had taken a quinolone, but 30/91 (33%) had travelled abroad (16 to the iberian peninsula) in the three months preceding isolation of the ciprofloxacin-resistant campylobacter spp. in the case-control study 12/15 (80%) of the cases had recently consumed poultry as had 20/24 (83%) of controls w ... | 1996 | 8722540 |
| rgfgigs is an amino acid sequence required for acetyl coenzyme a binding and activity of human spermidine/spermine n1acetyltransferase. | polyamine catabolism is rate limited by spermidine/spermine n1-acetyltransferase (ssat). although the amino acid sequence of ssat is known, the substrate binding and catalytic sites are not. the goal of this study was to define the region responsible for acetyl coenzyme a binding. human ssat contains a region of 20 amino acids homologous to several microbial antibiotic n-acetyltransferases. the highest homology is represented in the campylobacter coli streptothricin acetyltransferase sat4 gene, ... | 1996 | 8702554 |
| polymerase chain reaction assay for detection of campylobacter coli and campylobacter jejuni in poultry meat. | primers of 16s rrna and flaa genes were used to optimise a pcr technique for detecting thermotolerant campylobacters in poultry meat. different methods for crude dna extraction were also evaluated. the use of flaa primers and extraction of nucleic acid by boiling and proteinase k gave good results in the detection of campylobacter either in artificially or naturally contaminated foodstuffs. the lowest sensitivity limit for the pcr reaction was 10(1)-10(2) thermophilic campylobacter cells either ... | 1995 | 8672221 |
| ribotypes and ap-pcr fingerprints of thermophilic campylobacters from marine recreational waters. | thirty-two strains of thermophilic campylobacters isolated from marine recreational water and seven reference strains were biotyped and analysed by chromosomal dna haeiii ribopatterns and ap-pcr profiles based on a random 10-mer primer (5'-caa tcg ccg t-3'). the majority of seawater isolates (90%) were campylobacter coli, and three strains were camp. jejuni. southern blot hybridization analysis showed differences between the strains, and in a numerical analysis three main clusters were formed at ... | 1996 | 8642012 |
| [the comparative identification of campylobacter strains by traditional enzymatic tests and the gene amplification reaction]. | 39 strains of campylobacter isolated from 153 diarrhoeal children (0-3 years) were comparatively identified by the traditional enzymatic tests and by the polymerase chain reaction (pcr). the hippurate hydrolysis test appreciated 27 strains as campylobacter jejuni (69.2%) and 12 strains as campylobacter coli (30.8%). the p.c.r. realised in france has appreciated 29 strains as campylobacter jejuni (74.4%) and 10 strains as campylobacter coli (25.6%). the analysis of the results discrepancy permitt ... | 1995 | 8640005 |
| characterization of the type strain of campylobacter coli, cip 70.80, by plasmid typing. | a 1.9-kb plasmid dna fragment from the type strain of campylobacter coli, cip 70.80, was used as a probe to characterize this type strain, other c. coli type strains obtained from several culture collections, and other c. coli strains. a specific hybridization pattern was obtained, and this pattern can be used to identify, characterize, and follow up c. coli type strains in culture collections. | 1995 | 8590689 |
| enterochelin acquisition in campylobacter coli: characterization of components of a binding-protein-dependent transport system. | siderophore-mediated iron uptake systems play a central role in the pathogenesis of infection for many bacterial pathogens. campylobacter species are not thought to produce siderophores, yet they are able to utilize both ferrichrome and enterochelin as sources of iron. part of an operon named ceubcde, encoding components of a periplasmic binding-protein-dependent transport (pbt) system for the uptake of a ferric siderophore from campylobacter coli, was cloned directly into escherichia coli using ... | 1995 | 8574410 |
| the speciation and subtyping of campylobacter isolates from sewage plants and waste water from a connected poultry abattoir using molecular techniques. | in this study the distribution of phenotypes of campylobacter strains in sewage and surface waters was investigated by subtyping and by speciation of isolates from various aquatic environments. these environments included two municipal sewage plants (spa and spb) and waste water from a poultry abattoir (wwa). both the sewage plants spa and spb collected domestic and industrial waste, and spa received drain water from wwa. spb received no waste water from any meat-processing plant. the isolates w ... | 1995 | 8557080 |
| quinolone resistance in clinical strains of campylobacter jejuni and campylobacter coli. | 1995 | 8537280 | |
| arcobacter (campylobacter) butzleri-associated diarrheal illness in a nonhuman primate population. | after dna hybridization identified an isolate from an ill rhesus macaque (macaca mulatta) as arcobacter (campylobacter) butzleri, we initiated a study to determine whether a. butzleri was associated with diarrheal disease in nonhuman primates at the yerkes primate research center. by using campy-cva medium incubated at 35 degrees c, 15 a. butzleri isolates were obtained from 14 macaques; 7 macaques were coinfected with campylobacter coli and campylobacter jejuni. a. butzleri was not isolated fro ... | 1993 | 8478115 |
| the role of management systems in the epidemiology of thermophilic campylobacters among poultry in eastern zone of tanzania. | a total of 255 samples of droppings collected from a total of 22 different poultry units were examined for the presence of thermophilic campylobacters and the isolates biotyped using skirrow's protocol. the organisms were isolated from 90 (35.3%) of all samples. among the 22 units investigated, 13 (59%) were found to have unsatisfactory management systems, while 7 (32%) and 2 (9%) were found to have unsatisfactory and good systems respectively. significantly large numbers of isolations, 68 of 14 ... | 1993 | 8472771 |
| significance of duplicated flagellin genes in campylobacter. | the complex flagellum of campylobacter coli vc167 contains two highly related (98%) flagellin subunit proteins which are produced from two 92% homologous, tandemly orientated genes, flaa and flab. mutants expressing only flaa form a full-length flagellar filament that confers slightly less than wild-type motility to the bacterium. however, flagellin mutants expressing only flab produce extremely short, truncated filaments, and are only slightly motile. we have shown that the presence of two esse ... | 1993 | 8464049 |
| evaluation of commercial antisera for serotyping heat-labile antigens of campylobacter jejuni and campylobacter coli. | commercial antisera for serotyping 22 heat-labile antigens of campylobacter jejuni and campylobacter coli were evaluated by using 66 isolates from human and nonhuman sources. test results were compared with results of tests using antisera produced at the centers for disease control (cdc), atlanta, ga. all strains (three isolates of each of the 22 serotypes) were typeable with the cdc antisera. of 66 test strains, 39 (59%) were typed as the same serotype with both sets of antisera. twenty-four st ... | 1993 | 8463402 |
| lipopolysaccharide of campylobacter coli serotype o:30. fractionation and structure of liberated core oligosaccharide. | lipopolysaccharide from phenol-water extraction of cells of campylobacter coli serotype o:30 was separated as a water-insoluble gel of low m(r) from a water-soluble antigenic polysaccharide of high m(r). acetic acid hydrolysis of the ketosidic linkages to lipid a in the lipopolysaccharide furnished a core oligosaccharide. structural investigations were performed using 1h and 13c nmr, fast atom bombardment-mass spectrometry of permethylated derivatives, and methylation linkage analysis on the cor ... | 1993 | 8454598 |
| effect of host lineage on the virulence of campylobacter jejuni/coli in the chick embryo model. | the chorioallantoic membrane (cam) inoculated chick embryo model was used to study the effect of host lineage on the virulence of campylobacter jejuni and campylobacter coli. ld50 values were used to compare the susceptibilities of chick embryos from eight inbred chicken lines to infection by four strains of c. jejuni and one strain of c. coli. differences in susceptibility were found between inbred chicken lines. these were shown not to be due to maternal antibody status, nor transfer of antibo ... | 1993 | 8454192 |
| characterization of viable but nonculturable stage of c. coli, characterized with respect to electron microscopic findings, whole cell protein and lipooligosaccharide (los) patterns. | campylobacter coli ck 205, isolated from swine feces, was examined for changes in cell morphology, protein and lipooligosaccharide (los) patterns during starvation-survival experiments. bottles filled with sterile filtered a. dest. were seeded with campylobacters and incubated at 4 degrees c and 37 degrees c. transition to the nonculturable stage occurred within 48 hours (37 degrees c) and 2 weeks (4 degrees c), respectively. in contrast to the culturability the electrophoretic studies showed no ... | 1993 | 8451879 |
| heterologous gene expression in campylobacter coli: the use of bacterial luciferase in a promoter probe vector. | a novel promoter probe plasmid (psp73) was constructed to allow the analysis of environmentally regulated gene expression in campylobacter. the vector utilizes the luxab genes from xenorhabdus luminescens, which encode a thermostable luciferase, as reporters of gene expression. the utility of this reporter system was demonstrated by placing the expression of luxab under the transcriptional control of the flaa gene promoter. | 1993 | 8405932 |
| susceptibilities of beta-lactamase-positive and -negative strains of campylobacter coli to beta-lactam agents. | the percentages of susceptibility of 28 strains of campylobacter coli to beta-lactam agents were 96% for amoxicillin and ampicillin, 57% for ticarcillin, 4% for cefoxitin and cefuroxime, 61% for cefotaxime, and 11% for ceftazidime. none of the strains were susceptible to penicillin g, piperacillin, cefazolin, cephalothin, cefamandole, and cefoperazone. all strains were susceptible to imipenem and ciprofloxacin, and 21% were susceptible to erythromycin. a beta-lactamase was detected in 68% of the ... | 1993 | 8390812 |
| cloning and nucleotide sequence of the campylobacter jejuni gyra gene and characterization of quinolone resistance mutations. | the gyra gene of campylobacter jejuni ua580, which encodes the a subunit of dna gyrase, was cloned and its nucleotide sequence was determined. an open reading frame of 2,589 nucleotides was identified, which could code for a polypeptide of 863 amino acids with a m(r) of 97 kda. both the nucleotide sequence and the putative amino acid sequence show ca. 50% identity with those of other gyra genes from gram-positive and gram-negative bacteria. the locations of the gyra gene on genome maps of both c ... | 1993 | 8384814 |