Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antimicrobial resistance in campylobacter from broilers: association with production type and antimicrobial use. | the isolation and antimicrobial resistance of campylobacter jejuni and campylobacter coli strains from broilers arriving in french slaughterhouses, were analysed according to production types (i.e. standard, export or free-range) and antimicrobial (i.e. coccidiostats, growth promoters or therapeutic agents) administration in flocks. prevalence was 56.6% in standard, 51.3% in export and 80.0% in free-range broilers. three hundred and ninety-three strains were identified. two-thirds of the strains ... | 2003 | 14559174 |
| deletion of helicobacter pylori vacuolating cytotoxin gene by introduction of directed mutagenesis. | to construct a vaca-knockout helicobacter pylori mutant strain, whose only difference from the wild strain is its disrupted vaca gene. | 2003 | 14562387 |
| flagellin glycosylation island in pseudomonas syringae pv. glycinea and its role in host specificity. | the deduced amino acid sequences of the flagellins of pseudomonas syringae pv. tabaci and p. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. the reason for the difference seems to depend on the posttranslational modification of the flagellins. to investigate the role of this posttranslational modification in the interactions between plants and bacterial pathogens, we isolated genes that are potentially involved in the postt ... | 2003 | 14594840 |
| diversity and succession of the intestinal bacterial community of the maturing broiler chicken. | the diversity of bacterial floras in the ilea and ceca of chickens that were fed a vegetarian corn-soy broiler diet devoid of feed additives was examined by analysis of 1,230 partial 16s rrna gene sequences. nearly 70% of sequences from the ileum were related to those of lactobacillus, with the majority of the rest being related to clostridiaceae (11%), streptococcus (6.5%), and enterococcus (6.5%). in contrast, clostridiaceae-related sequences (65%) were the most abundant group detected in the ... | 2003 | 14602645 |
| a survey of campylobacter species shed in faeces of beef cattle using polymerase chain reaction. | a polymerase chain reaction (pcr)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. to ensure the removal of pcr inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control dna (i.e., dna designed to amplify with the campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. in only two samples (0.5%) were the internal control or campylobacter genu ... | 2003 | 14735214 |
| antimicrobial resistance in campylobacter jejuni and campylobacter coli strains isolated in 1991 and 2001-2002 from poultry and humans in berlin, germany. | the susceptibilities of 430 campylobacter jejuni strains and 79 c. coli strains to six antimicrobial agents were tested and analyzed. the two sets of strains originated from retail market chicken and turkey samples and from humans, respectively, in berlin, germany. two groups of isolates, one dating from 1991 and the other dating from 2001-2002, were tested. of the campylobacter sp. isolates recovered from humans in 2001-2002, 45.1% were resistant to ciprofloxacin, 37.8% were resistant to tetrac ... | 2003 | 14638490 |
| risk factors for sporadic campylobacter jejuni infections in rural michigan: a prospective case-control study. | this case-control study investigated risk factors for campylobacteriosis in a rural population. exposure to live farm animals was hypothesized to increase the risk for campylobacter jejuni enteritis. | 2003 | 14652344 |
| evaluation of the prospect microplate assay for detection of campylobacter: a routine laboratory perspective. | to evaluate the use of the new enzyme-linked immunosorbent assay, the prospect campylobacter microplate assay (alexon-trend, minneapolis, mn, usa), which allows 2-h detection of both campylobacter jejuni and campylobacter coli antigen directly in stool specimens. | 2003 | 14616724 |
| pseudaminic acid, the major modification on campylobacter flagellin, is synthesized via the cj1293 gene. | flagellins from campylobacter jejuni 81-176 and campylobacter coli vc167 are heavily glycosylated. the major modifications on both flagellins are pseudaminic acid (pse5ac7ac), a nine carbon sugar that is similar to sialic acid, and an acetamidino-substituted analogue of pseudaminic acid (pseam). previous data have indicated that pseam is synthesized via pse5ac7ac in c. jejuni 81-176, but that the two sugars are synthesized using independent pathways in c. coli vc167. the cj1293 gene of c. jejuni ... | 2003 | 14617187 |
| effect of temperature on viability of campylobacter jejuni and campylobacter coli on raw chicken or pork skin. | to determine growth and survival of campylobacter jejuni and campylobacter coli on chicken and pork, campylobacter spp. (10(4) cfu/cm2) were inoculated on pieces of raw, irradiated chicken or pork skin and exposed to temperatures ranging from -20 to 42 degrees c under either microaerobic or aerobic conditions. viable counts over 48 h declined 2 to 3 log cfu/cm2 at -20 degrees c and 1 to 2 log cfu/cm2 at 25 degrees c regardless of skin type, species of campylobacter, or level of oxygen. at 4 degr ... | 2003 | 14627278 |
| genetic diversity of campylobacter jejuni isolates from farm animals and the farm environment. | the genetic diversity of campylobacter jejuni isolates from farm animals and their environment was investigated by multilocus sequence typing (mlst). a total of 30 genotypes, defined by allelic profiles (assigned to sequence types [sts]), were found in 112 c. jejuni isolates originating in poultry, cattle, sheep, starlings, and slurry. all but two of these genotypes belonged to one of nine c. jejuni clonal complexes previously identified in isolates from human disease and retail food samples and ... | 2003 | 14660392 |
| isolation of campylobacter jejuni and campylobacter coli from the gall bladder samples of sheep and identification by polymerase chain reaction. | in this study, 100 gall bladder samples of sheep slaughtered at an abattoir in elaziğ province were examined for campylobacter jejuni and campylobacter coli by culture and polymerase chain reaction (pcr). preston campylobacter agar supplemented with 7% horse blood and preston selective supplement (oxoid, hampshire, uk) were used for isolation of the agents. campylobacter spp. were isolated in 66 samples, and they were identified as 34% c. jejuni and 32% c. coli. a multiplex pcr based upon the us ... | 2003 | 14629001 |
| survival of helicobacter pylori in a natural freshwater environment. | the mode by which helicobacter pylori, the causative agent of most gastric ulcers, is transmitted remains undetermined. epidemiological evidence suggests these organisms are waterborne; however, h. pylori has rarely been grown from potential water sources. this may be due to the ability of this organism to rapidly enter the viable but nonculturable (vbnc) state. our investigation examines the entrance of h. pylori into this state in laboratory cultures and a natural freshwater environment as wel ... | 2003 | 14660399 |
| avian reservoirs and zoonotic potential of the emerging human pathogen helicobacter canadensis. | a polyphasic identification approach was used to investigate the taxonomic position of campylobacter-like isolates recovered from barnacle geese (branta leucopsis) and canada geese (branta candensis). seven strains were selected from a collection of 21 isolates and analyzed by extensive phenotypic testing; four strains were characterized by 16s rrna gene sequence analysis. the results clearly identified the bird isolates as helicobacter canadensis, recently described as an emerging human pathoge ... | 2003 | 14660407 |
| applicability of a rapid duplex real-time pcr assay for speciation of campylobacter jejuni and campylobacter coli directly from culture plates. | a rapid duplex real-time polymerase chain reaction (pcr) assay for speciation of campylobacter jejuni and campylobacter coli using the abi prism 7700 sequence detection system (applied biosystems) was developed based on two of the genes used in a conventional multiplex pcr. a rapid turnaround time of 3 h was achieved with the use of boiled cell lysates. applicability of the assay was tested with 6015 random campylobacter strains referred to the campylobacter reference unit, with 97.6% being iden ... | 2003 | 14680705 |
| risk factors for antibiotic resistance in campylobacter spp. isolated from raw poultry meat in switzerland. | the world-wide increase of foodborne infections with antibiotic resistant pathogens is of growing concern and is designated by the world health organization as an emerging public health problem. thermophilic campylobacter have been recognised as a major cause of foodborne bacterial gastrointestinal human infections in switzerland and in many other countries throughout the world. poultry meat is the most common source for foodborne cases caused by campylobacter. because all classes of antibiotics ... | 2003 | 14662013 |
| fluoroquinolone-resistant campylobacter in animal reservoirs: dynamics of development, resistance mechanisms and ecological fitness. | thermophilic campylobacter species, including campylobacter jejuni and campylobacter coli, are responsible for foodborne campylobacteriosis in humans and are increasingly resistant to fluoroquinolone (fq) antimicrobials. the therapeutic use of fq antimicrobial agents in food animal production, particularly in poultry, has become a concern for public health, because the practice may promote the emergence of fq-resistant campylobacter that can be transmitted to humans through the food chain. recen ... | 2003 | 15134291 |
| routes for campylobacter contamination of poultry meat: epidemiological study from hatchery to slaughterhouse. | from april 1998 to march 2000, 18 broiler flocks were followed from the hatchery to the slaughterhouse. campylobacter was not found in the hatchery, 1-day-old chicks or in the rearing house before the arrival of the chicks. the infection of broiler flocks increased continuously during the rearing time, with a total of seven positive flocks at the end of rearing. farms with campylobacter-positive broilers were characterized by the circulation of campylobacter in the environment (puddles, dung hil ... | 2003 | 14959785 |
| cecal colonization of chicks by bovine-derived strains of campylobacter. | campylobacter jejuni and campylobacter coli strains were isolated from feces of dairy cattle at farms with no known problem due to campylobacteria. farms were located in the northeast, desert southwest, and pacific west. twenty isolates were identified by ribotyping with a riboprinter. the ability of these bovine isolates to colonize the ceca of chicks was determined by challenge inoculation and reisolation of the challenge strain from the ceca at 1 and 2 wk after challenge. isolates recovered f ... | 2003 | 14708992 |
| investigation of infectious agents associated with arthritis by reverse transcription pcr of bacterial rrna. | in reactive and postinfectious arthritis the joints are generally sterile but the presence of bacterial antigens and nucleic acids has been reported. to investigate whether organisms traffic to affected joints in these conditions, we performed reverse transcription pcr using universal primers to amplify any bacterial 16s rrna sequences present in synovial fluid. bacterial sequences were detected in most cases, even after treatment of the synovial fluid with dnase, implying the presence of bacter ... | 2003 | 12716447 |
| detection of conserved n-linked glycans and phase-variable lipooligosaccharides and capsules from campylobacter cells by mass spectrometry and high resolution magic angle spinning nmr spectroscopy. | glycomics, the study of microbial polysaccharides and genes responsible for their formation, requires the continuous development of rapid and sensitive methods for the identification of glycan structures. in this study, methods for the direct analysis of sugars from 108 to 1010 cells are outlined using the human gastrointestinal pathogen, campylobacter jejuni. using capillary-electrophoresis coupled with sensitive electrospray mass spectrometry, we demonstrate variability in the lipid a componen ... | 2003 | 12716884 |
| antimicrobial-resistant campylobacter species from retail raw meats. | the antimicrobial susceptibilities of 378 campylobacter isolates were determined. resistance to tetracycline was the most common (82%), followed by resistance to doxycycline (77%), erythromycin (54%), nalidixic acid (41%), and ciprofloxacin (35%). campylobacter coli displayed significantly higher rates of resistance to ciprofloxacin and erythromycin than campylobacter jejuni, and campylobacter isolates from turkey meat showed a greater resistance than those from chicken meat. | 2003 | 12732579 |
| phenotypic and genotypic differentiation of campylobacter spp. isolated from austrian broiler farms: a comparison. | an identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (pcr-rflp) was developed to differentiate isolates of the genera campylobacter, arcobacter and helicobacter. based on the 16s rrna gene of these genera, pcr amplified a 1216-bp fragment. the amplicons were digested with the restriction enzymes rsai and ecorv. additional differentiation was obtained using a pcr-assay based on the hippuricase gene. genotyping was performed on several re ... | 2003 | 12745378 |
| comparison of recovery methods for freeze-injured listeria monocytogenes, salmonella typhimurium, and campylobacter coli in cell suspensions and associated with pork surfaces. | cells injured as a result of freezing, heating, and acidification treatments may not grow during conventional microbiological procedures owing to the presence of selective agents, compounds, or dyes in the media, impairing the cell's ability to repair itself and grow. injured cells can be recovered by combining selective and nonselective media into a single system. with such combinations, the diffusion of the selective compounds or dyes is controlled, allowing for the resuscitation of injured ce ... | 2003 | 12747688 |
| pcr detection of seven virulence and toxin genes of campylobacter jejuni and campylobacter coli isolates from danish pigs and cattle and cytolethal distending toxin production of the isolates. | to study the prevalence of seven virulence and toxin genes, and cytolethal distending toxin (cdt) production of campylobacter jejuni and c. coli isolates from danish pigs and cattle. | 2003 | 12752808 |
| reduction of bacteria on pork carcasses associated with chilling method. | in addition to reducing the temperature of pork carcasses immediately after slaughter and before fabrication, blast chilling (snap chill) or conventional chilling can reduce bacterial populations associated with fresh meats. however, there is little information on bacteria survival resulting from the freeze or chill injury of meat products. in this study, porcine fecal slurries with and without pathogens (listeria monocytogenes, salmonella typhimurium, and campylobacter coli) were inoculated ont ... | 2003 | 12801003 |
| molecular typing of campylobacter jejuni and campylobacter coli. | 2003 | 12624086 | |
| antibiotic susceptibility of campylobacter jejuni and campylobacter coli human isolates from bosnia and herzegovina. | 2003 | 12654755 | |
| molecular typing of campylobacter jejuni isolates involved in a neonatal outbreak indicates nosocomial transmission. | genotypic typing by restriction fragment length polymorphism and pulsed-field gel electrophoresis showed that two neonates in a neonatal ward were infected with the same campylobacter jejuni strain. isolates from the mother and brother of the index patient were identical to each other but distinct from the neonatal type. genotyping results therefore suggested that the neonatal c. jejuni infection was nosocomial in origin. | 2003 | 12904420 |
| antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates from broiler chickens isolated at an irish poultry processing plant. | the antibiotic susceptibility of campylobacter jejuni and campylobacter coli isolates from broiler chickens were determined in order to evaluate the level of antibiotic resistance of campylobacter species in the irish poultry industry. | 2003 | 12680938 |
| antimicrobial susceptibilities of campylobacter strains isolated from finnish subjects infected domestically or from those infected abroad. | the in vitro susceptibilities of 678 campylobacter jejuni and campylobacter coli strains isolated from stool samples of the same number of finnish subjects were studied. a total of 523 patients, representing inhabitants from throughout finland, had not traveled abroad within the 2 weeks prior to becoming ill, whereas 155 persons had presumably acquired their infections abroad. the antimicrobial agents studied were erythromycin, ciprofloxacin, levofloxacin, trovafloxacin, and moxifloxacin. the mi ... | 2003 | 12499176 |
| survival and resuscitation of ten strains of campylobacter jejuni and campylobacter coli under acid conditions. | the culturability of 10 strains of campylobacter jejuni and campylobacter coli was studied after the bacteria were exposed to acid conditions for various periods of time. campylobacter cells could not survive 2 h under acid conditions (formic acid at ph 4). the 10 campylobacter strains could not be recovered, even when enrichment media were used. viable cells, however, could be detected by a double-staining (5-cyano-2,3-ditolyl tetrazolium chloride [ctc]-4',6'-diamidino-2-phenylindole [dapi]) te ... | 2003 | 12514068 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| the gam protein of bacteriophage mu is an orthologue of eukaryotic ku. | mu bacteriophage inserts its dna into the genome of host bacteria and is used as a model for dna transposition events in other systems. the eukaryotic ku protein has key roles in dna repair and in certain transposition events. here we show that the gam protein of phage mu is conserved in bacteria, has sequence homology with both subunits of ku, and has the potential to adopt a similar architecture to the core dna-binding region of ku. through biochemical studies, we demonstrate that gam and the ... | 2003 | 12524520 |
| the iron-binding protein dps confers hydrogen peroxide stress resistance to campylobacter jejuni. | we identified and characterized the iron-binding protein dps from campylobacter jejuni. electron microscopic analysis of this protein revealed a spherical structure of 8.5 nm in diameter, with an electron-dense core similar to those of other proteins of the dps (dna-binding protein from starved cells) family. cloning and sequencing of the dps-encoding gene (dps) revealed that a 450-bp open reading frame (orf) encoded a protein of 150 amino acids with a calculated molecular mass of 17,332 da. ami ... | 2003 | 12533477 |
| use of pcr for direct detection of campylobacter species in bovine feces. | this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ... | 2003 | 12788747 |
| rapid detection of campylobacter coli, c. jejuni, and salmonella enterica on poultry carcasses by using pcr-enzyme-linked immunosorbent assay. | contamination of retail poultry by campylobacter spp. and salmonella enterica is a significant source of human diarrheal disease. isolation and identification of these microorganisms require a series of biochemical and serological tests. in this study, campylobacter ceue and salmonella inva genes were used to design probes in pcr-enzyme-linked immunosorbent assay (elisa), as an alternative to conventional bacteriological methodology, for the rapid detection of campylobacter jejuni, campylobacter ... | 2003 | 12788755 |
| evaluation of broiler litter with reference to the microbial composition as assessed by using 16s rrna and functional gene markers. | very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. we evaluated the bacterial composition of poultry litter by using a combination of culture and molecular detection. total aerobic bacteria in poultry litter were detected by culture at 10(9) cfu/g of material. enteric bacteria such as enteroco ... | 2003 | 12571010 |
| molecular characterization of fluoroquinolone-resistant campylobacter spp. isolated from poultry. | campylobacteriosis, an infectious disease caused by campylobacter jejuni and campylobacter coli, is treated by fluoroquinolone antibiotics in clinical practices. however, use of these drugs in animal husbandry may select for fluoroquinolone-resistant campylobacters and, thereby, compromise the clinical treatment of infection. in this study, 21 fluoroquinolone-resistant campylobacters were isolated from poultry samples. morphological and biochemical characteristics indicated that 19 isolates were ... | 2003 | 12619802 |
| characterization of helicobacter pylori nickel metabolism accessory proteins needed for maturation of both urease and hydrogenase. | previous studies demonstrated that two accessory proteins, hypa and hypb, play a role in nickel-dependent maturation of both hydrogenase and urease in helicobacter pylori. here, the two proteins were purified and characterized. hypa bound two ni(2+) ions per dimer with positive cooperativity (hill coefficient, approximately 2.0). the dissociation constants k(1) and k(2) for ni(2+) were 58 and 1.3 microm, respectively. studies on purified site-directed mutant proteins in each of the five histidin ... | 2003 | 12533448 |
| detection and typing of campylobacter jejuni and campylobacter coli and analysis of indicator organisms in three waterborne outbreaks in finland. | waterborne outbreaks associated with contamination of drinking water by campylobacter jejuni are rather common in the nordic countries sweden, norway, and finland, where in sparsely populated districts groundwater is commonly used without disinfection. campylobacters, escherichia coli, or other coliforms have rarely been detected in potential sources. we studied three waterborne outbreaks in finland caused by c. jejuni and used sample volumes of 4,000 to 20,000 ml for analysis of campylobacters ... | 2003 | 12620821 |
| comparison of broth microdilution, e test, and agar dilution methods for antibiotic susceptibility testing of campylobacter jejuni and campylobacter coli. | a standardized broth microdilution method was compared to the e test and an agar dilution method for the antimicrobial susceptibility testing of campylobacter jejuni and c. coli isolates. a group of 47 human clinical isolates, 37 isolates from retail poultry, and 29 isolates from living turkeys (total, 113 isolates) was included in the study. these encompassed 92 c. jejuni and 21 c. coli strains. the mics of six antimicrobial agents were determined by the broth microdilution and e test methods, ... | 2003 | 12624030 |
| helicobacter pylori preferentially induces interleukin 12 (il-12) rather than il-6 or il-10 in human dendritic cells. | dendritic cells are potent antigen-presenting cells that are present in the gastrointestinal tract and are required for the induction of a th1 t-cell acquired immune response. since infection with the gastric pathogen helicobacter pylori elicits a th1 cell response, the interaction of these organisms with dendritic cells should reflect the th1 bias. we incubated h. pylori with cultured human dendritic cells and measured the cytokine induction profile, comparing the response to that induced by sa ... | 2003 | 12819109 |
| campylobacter coli - an important foodborne pathogen. | campylobacters are the most common bacterial cause of infectious intestinal disease (iid) in temperate countries. c. jejuni is the predominant cause of campylobacter iid, but the impact of other, less prevalent species has largely been ignored. here, we present estimates of the burden of indigenously acquired foodborne disease (ifd) due to campylobacter coli, the second most common cause of human campylobacteriosis. | 2003 | 12850159 |
| occurrence and resistance to antibiotics of campylobacter jejuni and campylobacter coli in animals and meat in northeastern italy. | a study was carried out in northeastern italy during 2000 and 2001 to investigate the occurrence of campylobacter jejuni and campylobacter coli in animals, cattle, pigs, and broilers, and raw meat, beef, pork, and chicken. campylobacter spp. were detected in 53.9% of the cattle, 63.5% of the pigs, and 82.9% of the broilers examined. chicken meat was frequently contaminated (81.3%), while lower rates were found in pork meat (10.3%) and beef (1.3%). the resistance to antibiotics of the strains was ... | 2003 | 12593931 |
| comparative genotyping of campylobacter jejuni by amplified fragment length polymorphism, multilocus sequence typing, and short repeat sequencing: strain diversity, host range, and recombination. | three molecular typing methods were used to study the relationships among 184 campylobacter strains isolated from humans, cattle, and chickens. all strains were genotyped by amplified fragment length polymorphism (aflp) analysis, multilocus sequence typing (mlst), and sequence analysis of a genomic region with short tandem repeats designated clustered regularly interspaced short palindromic repeats (crisprs). mlst and aflp analysis yielded more than 100 different profiles and patterns, respectiv ... | 2003 | 12517820 |
| inhibition of helicobacter pylori growth in vitro by bulgarian propolis: preliminary report. | bee glue (propolis) possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. the aim of the study was to evaluate the inhibitory effect of bulgarian propolis on helicobacter pylori growth in vitro. activity of 30% ethanolic extract of propolis (eep) against 38 clinical isolates of h. pylori was evaluated by using the agar-well diffusion method. ethanol was used as a control. in addition, the effect of propolis on the growth of 26 h. pylori and 18 campylobacter st ... | 2003 | 12721318 |
| in vivo tracking of campylobacter jejuni by using a novel recombinant expressing green fluorescent protein. | campylobacter jejuni is a leading cause of food-borne disease in developed countries. the goal of this study was to develop a plasmid-based reporter system with green fluorescent protein (gfp) to facilitate the study of c. jejuni in a variety of niches. c. jejuni transformants harboring the pmek91 gfp gene (gfp)-containing vector were readily detectable by both fluorescence microscopy and flow cytometry. given the ease of detecting these organisms, additional experiments were performed in which ... | 2003 | 12732559 |
| a real-time pcr assay for the detection of campylobacter jejuni in foods after enrichment culture. | a real-time pcr assay was developed for the quantitative detection of campylobacter jejuni in foods after enrichment culture. the specificity of the assay for c. jejuni was demonstrated with a diverse range of campylobacter species, related organisms, and unrelated genera. the assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. the assay was used to detect c. jejuni in both naturally and artificially contami ... | 2003 | 12620820 |
| epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated in québec, canada, from 1983 to 2000. | the epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated from 103 patients from 1983 to 2000 in québec, canada, were determined. the median number of patients infected annually with this bacteria was seven, with an incidence of 0.1 per 100,000 population. the male-to-female ratio was 1.1 to 1.0. the patients originated from 13 of the 18 québec socioeconomic regions. the age range of the patients was 6 months to 90 years old, 53% being > or = 70 ... | 2003 | 12517895 |
| pcr-restriction fragment length polymorphism analysis for detection of point mutations associated with macrolide resistance in campylobacter spp. | a 23s rrna gene fragment in domain v was sequenced from 30 clinical isolates of campylobacter spp., including 22 resistant to macrolides. two point mutations associated with erythromycin resistance were identified at positions 2074 and 2075 on the 23s rrna gene (homologous to a2142c and a2143g mutations in helicobacter pylori) in which an adenine residue is also replaced with a cytosine and a guanine residue, respectively. a combined pcr-restriction fragment length polymorphism technique was dev ... | 2003 | 12604552 |
| specific detection of arcobacter and campylobacter strains in water and sewage by pcr and fluorescent in situ hybridization. | the aim of this study was to evaluate pcr and fluorescent in situ hybridization (fish) techniques for detecting arcobacter and campylobacter strains in river water and wastewater samples. both 16s and 23s rrna sequence data were used to design specific primers and oligonucleotide probes for pcr and fish analyses, respectively. in order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on ... | 2003 | 12571045 |
| real-time pcr assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in helicobacter pylori. | the main cause of failure of helicobacter pylori eradication therapy is resistance to clarithromycin. the resistance is due to three point mutations in two positions on the 23s rrna (a2142c, a2142g, and a2143g). our aim was to develop a rapid and accurate method to detect these mutations directly on biopsy specimens. we developed a real-time pcr that included a simultaneous detection of the amplicons by hybridization of two probes labeled with lc-red and fluorescein by using the fluorescence res ... | 2003 | 12517879 |
| molecular assays for detection of human metapneumovirus. | the recent description of the respiratory pathogen human metapneumovirus (hmpv) has highlighted a deficiency in current diagnostic techniques for viral agents associated with acute lower respiratory tract infections. we describe two novel approaches to the detection of viral rna by use of reverse transcriptase pcr (rt-pcr). the pcr products were identified after capture onto a solid-phase medium by hybridization with a sequence-specific, biotinylated oligonucleotide probe. the assay was applied ... | 2003 | 12517833 |
| enhanced surveillance of campylobacter infection in the north west of england 1997-1999. | to identify sources and routes of infection for sporadic cases of campylobacter infection in the north west of england. | 2003 | 12504607 |
| antimicrobial susceptibility trends in campylobacter jejuni and campylobacter coli isolated from a rural egyptian pediatric population with diarrhea. | comparative and trend analysis was conducted on annual prevalence of antimicrobial susceptibility among campylobacter jejuni and campylobacter coli recovered from rural egyptian children from 1995 through 2000. c. jejuni and c. coli demonstrated significant decreasing trends in ciprofloxacin susceptibility over the study period (p < 0.001 for both). in general, c. coli demonstrated a higher degree of susceptibility than c. jejuni, however, there was no statistical difference (p = 0.2) comparing ... | 2003 | 14711482 |
| risk factors associated with campylobacter jejuni infections in curaçao, netherlands antilles. | a steady increase in the incidence of guillain-barré syndrome (gbs) with a seasonal preponderance, almost exclusively related to campylobacter jejuni, and a rise in the incidence of laboratory-confirmed campylobacter enteritis have been reported from curaçao, netherlands antilles. we therefore investigated possible risk factors associated with diarrhea due to epidemic c. jejuni. typing by pulsed-field gel electrophoresis identified four epidemic clones which accounted for almost 60% of the infec ... | 2003 | 14662945 |
| birds, migration and emerging zoonoses: west nile virus, lyme disease, influenza a and enteropathogens. | wild birds are important to public health because they carry emerging zoonotic pathogens, either as a reservoir host or by dispersing infected arthropod vectors. in addition, bird migration provides a mechanism for the establishment of new endemic foci of disease at great distances from where an infection was acquired. birds are central to the epidemiology of west nile virus (wnv) because they are the main amplifying host of the virus in nature. the initial spread of wnv in the u.s. along the ea ... | 2003 | 15931279 |
| frequency and spatial distribution of environmental campylobacter spp. | humans are exposed to campylobacter spp. in a range of sources via both food and environmental pathways. for this study, we explored the frequency and distribution of thermophilic campylobacter spp. in a 10- by 10-km square rural area of cheshire, united kingdom. the area contains approximately 70, mainly dairy, farms and is used extensively for outdoor recreational activities. campylobacter spp. were isolated from a range of environmental samples by use of a systematic sampling grid. livestock ... | 2004 | 15528512 |
| proteomic analysis of the sarcosine-insoluble outer membrane fraction of helicobacter pylori strain 26695. | helicobacter pylori causes gastroduodenal disease, which is mediated in part by its outer membrane proteins (omps). to identify omps of h. pylori strain 26695, we performed a proteomic analysis. a sarcosine-insoluble outer membrane fraction was resolved by two-dimensional electrophoresis with immobilized ph gradient strips. most of the protein spots, with molecular masses of 10 to 100 kda, were visible on the gel in the alkaline pi regions (6.0 to 10.0). the proteome of the omps was analyzed by ... | 2004 | 14761989 |
| detection of campylobacter spp. in chicken fecal samples by real-time pcr. | a real-time pcr assay for detecting thermophilic campylobacter spp. directly in chicken feces has been developed. dna was isolated from fecal material by using magnetic beads followed by pcr with a prealiquoted pcr mixture, which had been stored at -18 degrees c. campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 cfu/ml, in a fecal suspension. a bacterial internal control was added before dna extraction to control both dna isolation and the presence of pcr inh ... | 2004 | 15528705 |
| antimicrobial resistance in campylobacter strains isolated from french broilers before and after antimicrobial growth promoter bans. | the antimicrobial susceptibility of campylobacter strains isolated from standard and free-range broilers in 1992-1996 and 2001-2002 was studied. | 2004 | 15537699 |
| insufficient production and tissue delivery of cd4+ memory t cells in rapidly progressive simian immunodeficiency virus infection. | the mechanisms linking human immunodeficiency virus replication to the progressive immunodeficiency of acquired immune deficiency syndrome are controversial, particularly the relative contribution of cd4+ t cell destruction. here, we used the simian immunodeficiency virus (siv) model to investigate the relationship between systemic cd4+ t cell dynamics and rapid disease progression. of 18 rhesus macaques (rms) infected with ccr5-tropic sivmac239 (n=14) or cxcr4-tropic sivmac155t3 (n=4), 4 of the ... | 2004 | 15545355 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| antimicrobial resistance among enteric pathogens. | diarrhea is a major cause of morbidity and mortality in infants and children worldwide. use of antimicrobial therapy in children with bacterial diarrhea involves consideration of advantages and limitations of use of appropriate agents in the general population and in specific hosts. antimicrobial agents for bacterial diarrhea should be prescribed with an appreciation of limitations including antimicrobial resistance. studies from many countries have reported resistance to campylobacter jejuni, c ... | 2004 | 15185189 |
| characterization of erythromycin resistance in campylobacter coli and campylobacter jejuni isolated from pig offal in new zealand. | to determine the level and mechanism(s) of antimicrobial resistance in campylobacter isolates obtained from human and environmental sources from south canterbury, new zealand. | 2004 | 15186451 |
| characterization of campylobacter isolates recovered from clinically healthy pigs and from sporadic cases of campylobacteriosis in humans. | campylobacter spp. were recovered from 660 (77.6%) of 850 swine cecal contents at the abattoir and from 24 (8.6%) of 278 specimens from sporadic cases of human diarrhea during the same period in the same geographical area. campylobacter coli represented 95.7% of campylobacter isolates recovered from pigs and 8.3% of those isolated from humans. genetic profiles were determined by pulsed-field gel electrophoresis (pfge) using kpni enzyme to characterize the isolates in combination with phenotypic ... | 2004 | 14968951 |
| distribution and characterization of campylobacter spp. from russian poultry. | the distribution of campylobacter spp. on 13 poultry farms (broiler chicken, quail, pheasant, peacock, and turkey) from eight regions (vladimir, vologda, voronezh, kaluga, liptsk, moscow, orenburg, and orel) in russia was surveyed. intestinal materials were plated onto campylobacter-selective medium and plates were incubated microaerobically at 42 degrees c for 24 or 48 h. identification was based on colonial morphology, microscopic examination, and biochemical tests; latex agglutination assays ... | 2004 | 14968953 |
| relapsing cellulitis associated with campylobacter coli bacteremia in an agammaglobulinemic patient. | campylobacter coli rarely causes bacteremia or extraintestinal infection. we report herein a case of agammaglobulinemia in which cellulitis associated with c. coli bacteremia relapsed after a disease-free interval of >5 years. pulsed field gel electrophoresis revealed that the organisms in this patient were genetically identical, suggesting a latent c. coli infection. | 2004 | 15194845 |
| optimization of pcr-based methods for rapid detection of campylobacter jejuni, campylobacter coli and yersinia enterocolitica serovar 0:3 in wastewater samples. | pcr-based methods were evaluated for their adequacy to assess the removal of pathogens from wastewater samples. for the development and optimization of the methods, samples were taken at two different sites from two different constructed wetlands. campylobacter jejuni/coli and yersinia enterocolitica serogroup 0:3 were selected as model pathogens and enterococcus faecalis as a standard microbiological indicator. the chosen pcr protocols were optimized for wastewater dna extracts in order to obta ... | 2004 | 14975667 |
| helicobacter pylori induces apoptosis of human monocytes but not monocyte-derived dendritic cells: role of the cag pathogenicity island. | monocytes are circulating precursors of the dendritic cell subset, professional antigen-presenting cells with a unique ability to initiate the innate and adaptive immune response. in this study, we have investigated the effects of wild-type helicobacter pylori strains and their isogenic mutants with mutations in known bacterial virulence factors on monocytes and monocyte-derived dendritic cells. we show that h. pylori strains induce apoptosis of human monocytes by a mechanism that is dependent o ... | 2004 | 15271906 |
| antimicrobial susceptibilities of campylobacter isolated from food-producing animals on farms (1999-2001): results from the japanese veterinary antimicrobial resistance monitoring program. | nationwide monitoring of antimicrobial resistance in campylobacter isolated from food-producing animals on farms was performed in japan. a total of 468 campylobacter isolates were obtained during the period from june 1999 to march 2001. campylobacter species showed high frequencies of resistance to oxytetracycline and dihydrostreptomycin. the frequencies of resistance in campylobacter coli to aminoglycosides, macrolides, tetracycline and quinolones were higher than those in campylobacter jejuni ... | 2004 | 15325430 |
| use of midi-fatty acid methyl ester analysis to monitor the transmission of campylobacter during commercial poultry processing. | the presence of campylobacter spp. on broiler carcasses and in scald water taken from a commercial poultry processing facility was monitored on a monthly basis from january through june. campylobacter agar, blaser, was used to enumerate campylobacter in water samples from a multiple-tank scalder; on prescalded, picked, eviscerated, and chilled carcasses; and on processed carcasses stored at 4 degrees c for 7 or 14 days. the midi sherlock microbial identification system was used to identify campy ... | 2004 | 15330523 |
| global transposon mutagenesis and essential gene analysis of helicobacter pylori. | we have constructed a genome-saturating mutant library of the human gastric pathogen helicobacter pylori. microarray tracking of transposon mutants (matt) allowed us to map the position of 5,363 transposon mutants in our library. while we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes. comparison to the essential gene set of other bacteria revealed a surpris ... | 2004 | 15547264 |
| species identification by genotyping and determination of antibiotic resistance in campylobacter jejuni and campylobacter coli from humans and chickens in sweden. | campylobacter is today the most common cause of human bacterial enteritis in sweden, as well as in most other industrialized countries. common sources of infection are undercooked chicken meat, unpasteurized milk and contaminated drinking water. one aim with our present study was to identify the species campylobacter jejuni and campylobacter coli strains from humans and chickens using a polymerase chain reaction/restriction enzyme analysis (pcr/rea) method, as well as traditional hippurate hydro ... | 2004 | 15364471 |
| use of culture, pcr analysis, and dna microarrays for detection of campylobacter jejuni and campylobacter coli from chicken feces. | a dna microarray for detection of campylobacter spp. was recently developed and applied to detect campylobacter spp. directly from chicken feces. sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. the results of campylobacter sp. detection obtained with dna microarrays were compared to those obtained by conventional culture and gel electrophoresis. by conventional culture, 60% of the samples were positive for either campylobacter jejun ... | 2004 | 15364980 |
| campylobacter, from obscurity to celebrity. | after its successful isolation from stools in the 1970s, campylobacter jejuni has rapidly become the most commonly recognised cause of bacterial gastroenteritis in man. reported cases of human campylobacteriosis represent only a small fraction of the actual number. in industrialised countries, the incidence of c. jejuni/campylobacter coli infections peaks during infancy, and again in young adults aged 15-44 years. acute self-limited gastrointestinal illness, characterised by diarrhoea, fever and ... | 2004 | 15373879 |
| nucleotide sequences and comparison of two large conjugative plasmids from different campylobacter species. | two large tetracycline resistance (tcr) plasmids have been completely sequenced, the ptet plasmid (45.2 kb) from campylobacter jejuni strain 81-176 and a plasmid pcc31 (44.7 kb) from campylobacter coli strain cc31 that was isolated from a human case of severe gastroenteritis in the uk. both plasmids are mosaic in structure, having homologues of genes found in a variety of different commensal and pathogenic bacteria, but nevertheless, showed striking similarities in dna sequence and overall gene ... | 2004 | 15470128 |
| swimming and campylobacter infections. | a matched case-control study was conducted to study risk factors for domestically acquired sporadic campylobacter infections in finland. swimming in natural sources of water was a novel risk factor. eating undercooked meat and drinking dug-well water were also independent risk factors for campylobacter infection. | 2004 | 15496253 |
| antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates in japan. | 2004 | 15499812 | |
| pcr-restriction fragment length polymorphism assay for detection of gyra mutations associated with fluoroquinolone resistance in campylobacter coli. | a fragment of the gyra gene was sequenced from 34 isolates of campylobacter coli, including 23 isolates resistant to ciprofloxacin. all ciprofloxacin-resistant isolates examined by dna sequencing carried a point mutation at position thr-86 on the gyra gene product, involving the replacement of thr-86 by ile. a combined pcr-restriction fragment length polymorphism technique using rsai was developed to detect this mutation. | 2004 | 15561873 |
| characterization of a haemolytic phospholipase a(2) activity in clinical isolates of campylobacter concisus. | a membrane-bound, haemolytic phospholipase a(2) (pla(2)) activity was detected in clinical strains of campylobacter concisus isolated from children with gastroenteritis. the clinical strains were assigned into two molecular groups (genomospecies) based on pcr amplification of their 23s rdna. this calcium-dependent, heat-stable, haemolytic pla(2) activity was detected in strains from both genomospecies. a crude haemolysin extract (che) was initially prepared from cellular outer-membrane proteins ... | 2004 | 15150326 |
| sensitive and specific detection of yersinia pseudotuberculosis by loop-mediated isothermal amplification. | we developed a loop-mediated isothermal amplification method able to detect yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. this method is more sensitive than pcr and might be a useful tool for detecting and identifying y. pseudotuberculosis. | 2004 | 15528740 |
| iron acquisition and regulation in campylobacter jejuni. | iron affects the physiology of bacteria in two different ways: as a micronutrient for bacterial growth and as a catalyst for the formation of hydroxyl radicals. in this study, we used dna microarrays to identify the c. jejuni genes that have their transcript abundance affected by iron availability. the transcript levels of 647 genes were affected after the addition of iron to iron-limited c. jejuni cells. several classes of affected genes were revealed within 15 min, including immediate-early re ... | 2004 | 15231804 |
| genotyping of broiler-originated campylobacter jejuni and campylobacter coli isolates using fla typing and random amplified polymorphic dna methods. | liver and intestine samples taken from 200 broilers at 20 flocks were inoculated onto preston enrichment broth and agar for selective isolation of campylobacter jejuni and campylobacter coli. the isolates were identified by both conventional and polymerase chain reaction (pcr) methods. campylobacter spp. were identified in 102 of 400 samples (200 liver and 200 intestine), 57 (14.25%) of which were identified as c. jejuni and 45 (11.25%) as c. coli. pcr-restriction fragment length polymorphism (r ... | 2004 | 15193806 |
| direct quantification of campylobacter jejuni and campylobacter lanienae in feces of cattle by real-time quantitative pcr. | campylobacter species are fastidious to culture, and the ability to directly quantify biomass in microbiologically complex substrates using real-time quantitative (rtq) pcr may enhance our understanding of their biology and facilitate the development of efficacious mitigation strategies. this study reports the use of nested rtq-pcr to directly quantify campylobacter jejuni and campylobacter lanienae in cattle feces. for c. jejuni, the single-copy mapa gene was selected. for c. lanienae, the thre ... | 2004 | 15066825 |
| helicobacter pylori flgr is an enhancer-independent activator of sigma54-rna polymerase holoenzyme. | helicobacter pylori flgr activates transcription with sigma54-rna polymerase holoenzyme (sigma54-holoenzyme) from at least five flagellar operons. activators of sigma54-holoenzyme generally bind enhancer sequences located >70 bp upstream of the promoter and contact sigma54-holoenzyme bound at the promoter through dna looping to activate transcription. h. pylori flgr lacks the carboxy-terminal dna-binding domain present in most sigma54-dependent activators. as little as 42 bp of dna upstream of t ... | 2004 | 15231786 |
| identification of bacterial populations in dairy wastewaters by use of 16s rrna gene sequences and other genetic markers. | hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. although these systems are common, little is known about the microbial populations that inhabit them or how they change as they traverse the system. using culture-based and non-culture-based methods, we characterized the microbial community structure of manure, water from the separator p ... | 2004 | 15240310 |
| regional risks and seasonality in travel-associated campylobacteriosis. | the epidemiology of travel-associated campylobacteriosis is still largely unclear, and various known risk factors could only explain limited proportions of the recorded cases. | 2004 | 15569393 |
| diagnostic real-time pcr for detection of salmonella in food. | a robust 5' nuclease (taqman) real-time pcr was developed and validated in-house for the specific detection of salmonella in food. the assay used specifically designed primers and a probe target within the ttrrsbca locus, which is located near the salmonella pathogenicity island 2 at centisome 30.5. it is required for tetrathionate respiration in salmonella. the assay correctly identified all 110 salmonella strains and 87 non-salmonella strains tested. an internal amplification control, which is ... | 2004 | 15574899 |
| longitudinal study of campylobacter jejuni bacteriophages and their hosts from broiler chickens. | a longitudinal study of bacteriophages and their hosts was carried out at a broiler house that had been identified as having a population of campylobacter-specific bacteriophages. cloacal and excreta samples were collected from three successive broiler flocks reared in the same barn. campylobacter jejuni was isolated from each flock, whereas bacteriophages could be isolated from flocks 1 and 2 but were not isolated from flock 3. the bacteriophages isolated from flocks 1 and 2 were closely relate ... | 2004 | 15240258 |
| survival of cold-stressed campylobacter jejuni on ground chicken and chicken skin during frozen storage. | campylobacter jejuni is prevalent in poultry, but the effect of combined refrigerated and frozen storage on its survival, conditions relevant to poultry processing and storage, has not been evaluated. therefore, the effects of refrigeration at 4 degrees c, freezing at -20 degrees c, and a combination of refrigeration and freezing on the survival of c. jejuni in ground chicken and on chicken skin were examined. samples were enumerated using tryptic soy agar containing sheep's blood and modified c ... | 2004 | 15574906 |
| characterization of lactobacillus coryniformis dsm 20001t surface protein cpf mediating coaggregation with and aggregation among pathogens. | phenotypic characterization of aggregation phenotypes of lactobacillus coryniformis revealed that strain dsm 20001t coaggregated with escherichia coli k88, campylobacter coli, and campylobacter jejuni but not with other human pathogens. in addition, cells of these pathogens aggregated in the presence of the spent culture supernatant (scs) of strain dsm 20001t. cells of e. coli k88 remained viable in the coaggregates and aggregates for up to 24 h. both coaggregation and aggregation (co/aggregatio ... | 2004 | 15574903 |
| multiplex pcr assay for detection of streptococcus suis species and serotypes 2 and 1/2 in tonsils of live and dead pigs. | a pcr assay was developed for the detection of streptococcus suis serotypes 2 and 1/2. this multiplex pcr is based on the amplification of the gene coding for 16s rrna of s. suis and on the amplification of the cps2j gene coding for the capsule of s. suis serotypes 2 and 1/2. an internal control was constructed and added in this test to monitor the efficiency of amplification in each reaction. to evaluate the specificity of the test, 31 strains of other bacterial species related to s. suis or is ... | 2004 | 15243078 |
| characterization of plasmid-mediated apha-3 kanamycin resistance in campylobacter jejuni. | a total of 254 isolates of campylobacter jejuni and three isolates of campylobacter coli, isolated from sweden, canada, and egypt, were screened for kanamycin resistance. eight strains of c. jejuni contained large plasmids that carried the apha-3 kanamycin-resistance marker. in six plasmids, the apha-3 gene was located downstream of an apparent insertion sequence, designated is607*, which showed a considerable similarity to is607, characterized on the chromosome of some helicobacter pylori strai ... | 2004 | 15256024 |
| campylobacter colonization of sibling turkey flocks reared under different management conditions. | uncertainty exists concerning the key factors contributing to campylobacter colonization of poultry, especially the possible role of vertical transmission from breeder hens to young birds. a longitudinal study of campylobacter colonization was performed in two sibling pairs of turkey flocks (four flocks total). each pair of sibling flocks shared breeder hen populations and was obtained from the same hatchery. one flock of each pair was grown on a commercial farm, and the other was grown in an in ... | 2004 | 15270502 |
| campylobacter prevalence in lactating dairy cows in the united states. | the objective of the present study was to determine the prevalence of intestinal campylobacter in lactating dairy cows from various regions of the united states. participating commercial dairy farms were chosen at random and were part of a national survey to determine e. coli o157:h7 and salmonella prevalence in dairy cows. farms had no previous history of campylobacter problems. fecal samples were collected rectally from 720 cows on farms in the northeast (four farms), in the desert southwest ( ... | 2004 | 15270504 |
| a real-time multiplexed pcr assay for rapid detection and differentiation of campylobacter jejuni and campylobacter coli. | campylobacter species are the leading agents of bacterial gastroenteritis worldwide. c. jejuni and c. coli together are responsible for more than 95% of all cases of campylobacter-induced diarrheal disease in the united states. detection of campylobacteria in clinical samples by conventional culture is problematic and slow due to their complex taxonomy, fastidious growth requirements, and biochemical inertness. the current study describes a rapid, sensitive, and specific real-time polymerase cha ... | 2004 | 15271389 |
| campylobacter insulaenigrae sp. nov., isolated from marine mammals. | phenotypic and phylogenetic studies were performed on four campylobacter-like organisms recovered from three seals and a porpoise. comparative 16s rrna gene sequencing studies demonstrated that the organisms represent a hitherto unknown subline within the genus campylobacter, associated with a subcluster containing campylobacter jejuni, campylobacter coli and campylobacter lari. dna-dna hybridization studies confirmed that the bacteria belonged to a single species, for which the name campylobact ... | 2004 | 15545485 |
| molecular characterization of benzimidazole resistance in helicobacter pylori. | a family of benzimidazole derivatives (bi) was shown to possess potent and selective activity against helicobacter pylori, although the precise cellular target of the bis is unknown. spontaneous h. pylori mutants were isolated as resistant to a representative bi (compound a). genomic dna was isolated from a bi-resistant mutant, transformed into a bi-sensitive strain, and found to be sufficient to confer bi resistance. the resistance determinant was localized to a 17-kb clone after screening a la ... | 2004 | 15215104 |