Publications

virulence genes and cytokine profile in systemic murine campylobacter coli infection.	campylobacter coli are one of the most common bacteria in bacterial gastroenteritis and acute enterocolitis in humans. however, relatively little is known regarding the mechanisms of pathogenesis and host response to c. coli infections. to investigate the influence of genetic changes, we first used pcr to demonstrate the presence of the known virulence genes cadf, virb11, cdtb, cdtc and ceue in the clinical isolate c. coli 26536, which was isolated from the liver of infected balb/c mice. sequenc ...	2015	26039573
quantitative microbial risk assessment for campylobacter spp. on ham in korea.	the objective of this study was to evaluate the risk of illness from campylobacter spp. on ham. to identify the hazards of campylobacter spp. on ham, the general characteristics and microbial criteria for campylobacter spp., and campylobacteriosis outbreaks were investigated. in the exposure assessment, the prevalence of campylobacter spp. on ham was evaluated, and the probabilistic distributions for the temperature of ham surfaces in retail markets and home refrigerators were prepared. in addit ...	2015	26761897
agriculture and food animals as a source of antimicrobial-resistant bacteria.	one of the major breakthroughs in the history of medicine is undoubtedly the discovery of antibiotics. their use in animal husbandry and veterinary medicine has resulted in healthier and more productive farm animals, ensuring the welfare and health of both animals and humans. unfortunately, from the first use of penicillin, the resistance countdown started to tick. nowadays, the infections caused by antibiotic-resistant bacteria are increasing, and resistance to antibiotics is probably the major ...	2015	25878509
coiled coil rich proteins (ccrp) influence molecular pathogenicity of helicobacter pylori.	pathogenicity of the human pathogen helicobacter pylori relies on its capacity to adapt to a hostile environment and to escape the host response. although there have been great advances in our understanding of the bacterial cytoskeleton, major gaps remain in our knowledge of its contribution to virulence. in this study we have explored the influence of coiled coil rich proteins (ccrp) cytoskeletal elements on pathogenicity factors of h. pylori. deletion of any of the ccrp resulted in a strongly ...	2015	25822999
molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of red sea.	molecular-based approaches were used to characterize the coastal microbiota and to elucidate the trophic state of red sea. nutrient content and enterococci numbers were monitored, and used to correlate with the abundance of microbial markers. microbial source tracking revealed the presence of >1 human-associated bacteroides spp. at some of the near-shore sampling sites and at a heavily frequented beach. water samples collected from the beaches had occasional exceedances in enterococci numbers, h ...	2015	25758166
clear distinction between burkholderia mallei and burkholderia pseudomallei using fluorescent motb primers.	a frame-shift mutation in the flagellum motor gene motb coding for the chemotaxis motb protein of burkholderia mallei has been utilized to design a conventional duplex pcr assay with fluorescent labelled primers.	2015	25887130
multicenter evaluation of the bd max enteric bacterial panel pcr assay for rapid detection of salmonella spp., shigella spp., campylobacter spp. (c. jejuni and c. coli), and shiga toxin 1 and 2 genes.	diarrhea due to enteric bacterial pathogens causes significant morbidity and mortality in the united states and worldwide. however, bacterial pathogens may be infrequently identified. currently, culture and enzyme immunoassays (eias) are the primary methods used by clinical laboratories to detect enteric bacterial pathogens. we conducted a multicenter evaluation of the bd max enteric bacterial panel (ebp) pcr assay in comparison to culture for the detection of salmonella spp., shigella spp., cam ...	2015	25740779
chromosomal integration vectors allowing flexible expression of foreign genes in campylobacter jejuni.	campylobacter jejuni is a major cause of human gastroenteritis yet there is limited knowledge of how disease is caused. molecular genetic approaches are vital for research into the virulence mechanisms of this important pathogen. vectors that allow expression of genes in c. jejuni via recombination onto the chromosome are particularly useful for genetic complementation of insertional knockout mutants and more generally for expression of genes in particular c. jejuni host backgrounds.	2015	26497958
substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique n-glycosylation site preferences.	the central enzyme in the campylobacter jejuni asparagine-linked glycosylation pathway is the oligosaccharyltransferase (ost), pglb, which transfers preassembled glycans to specific asparagine residues in target proteins. while c. jejuni pglb (cjpglb) can transfer many diverse glycan structures, the acceptor sites that it recognizes are restricted predominantly to those having a negatively charged residue in the -2 position relative to the asparagine. here, we investigated the acceptor-site pref ...	2015	26482295
transmission of the pabi family of restriction dna glycosylase genes: mobility and long-term inheritance.	r.pabi is an exceptional restriction enzyme that functions as a dna glycosylase. the enzyme excises an unmethylated base from its recognition sequence to generate apurinic/apyrimidinic (ap) sites, and also displays ap lyase activity, cleaving the dna backbone at the ap site to generate the 3'-phospho alpha, beta-unsaturated aldehyde end in addition to the 5'-phosphate end. the resulting ends are difficult to religate with dna ligase. the enzyme was originally isolated in pyrococcus, a hypertherm ...	2015	26481899
the epidemiology and aetiology of diarrhoeal disease in infancy in southern vietnam: a birth cohort study.	previous studies indicate a high burden of diarrhoeal disease in vietnamese children, however longitudinal community-based data on burden and aetiology are limited. the findings from a large, prospective cohort study of diarrhoeal disease in infants in southern vietnam are presented herein.	2015	25813553
helicobacter pylori adaptation in vivo in response to a high-salt diet.	helicobacter pylori exhibits a high level of intraspecies genetic diversity. in this study, we investigated whether the diversification of h. pylori is influenced by the composition of the diet. specifically, we investigated the effect of a high-salt diet (a known risk factor for gastric adenocarcinoma) on h. pylori diversification within a host. we analyzed h. pylori strains isolated from mongolian gerbils fed either a high-salt diet or a regular diet for 4 months by proteomic and whole-genome ...	2015	26438795
pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in campylobacter jejuni.	campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by c. jejuni and that a secreted protease, cj0511, is required. exposure of c. jejuni to pancreatic amylase promotes biofilm formation in vitro, increases interaction with human epithelial cell lines, increases virulence in the galleria mellonella infection mode ...	2015	26438798
a metagenomic investigation of the duodenal microbiota reveals links with obesity.	few studies have tested the small intestine microbiota in humans, where most nutrient digestion and absorption occur. here, our objective was to examine the duodenal microbiota between obese and normal volunteers using metagenomic techniques.	2015	26356733
wild bird-associated campylobacter jejuni isolates are a consistent source of human disease, in oxfordshire, united kingdom.	the contribution of wild birds as a source of human campylobacteriosis was investigated in oxfordshire, united kingdom (uk) over a 10 year period. the probable origin of human campylobacter jejuni genotypes, as described by multilocus sequence typing, was estimated by comparison with reference populations of isolates from farm animals and five wild bird families, using the structure algorithm. wild bird-attributed isolates accounted for between 476 (2.1%) and 543 (3.5%) cases annually. this prop ...	2015	26109474
genetic characterization of campylobacter jejuni and c. coli isolated from broilers using flaa pcr-restriction fragment length polymorphism method in shiraz, southern iran.	thermophilic campylobacters, particularly campylobacter jejuni and c. coli are the main agents of human campylobacteriosis. campylobacter contaminated chicken products is the most important source of foodborne gastroenteritis. evaluation of genetic diversity among campylobacter population is critical for understanding the epidemiology of this bacterium and developing effective control strategies against campylobacter infections and other related disorders.	2015	26060566
laboratory diagnosis of bacterial gastroenteritis.	bacterial gastroenteritis is a disease that is pervasive in both the developing and developed worlds. while for the most part bacterial gastroenteritis is self-limiting, identification of an etiological agent by bacterial stool culture is required for the management of patients with severe or prolonged diarrhea, symptoms consistent with invasive disease, or a history that may predict a complicated course of disease. importantly, characterization of bacterial enteropathogens from stool cultures i ...	2015	25567220
isolation and molecular identification of potentially pathogenic escherichia coli and campylobacter jejuni in feral pigeons from an urban area in the city of lima, peru.	feral pigeons (columbia livia) live in close contact with humans and other animals. they can transmit potentially pathogenic and zoonotic agents. the objective of this study was to isolate and detect strains of diarrheagenic escherichia coli and campylobacter jejuni of urban feral pigeons from an area of lima, peru. fresh dropping samples from urban parks were collected for microbiological isolation of e. coli strains in selective agar, and campylobacter by filtration method. molecular identific ...	2015	26603225
epidemiology of norovirus infections among diarrhea outpatients in a diarrhea surveillance system in shanghai, china: a cross-sectional study.	norovirus is an important cause of gastroenteritis both in children and adults. in china, few studies have been conducted on adult populations. this study aimed to determine the contribution of norovirus to gastroenteritis, characterize the features of norovirus infections, compare them with other pathogens, and test the effectiveness of the surveillance system.	2015	25884557
filamentation of campylobacter in broth cultures.	the transition from rod to filamentous cell morphology has been identified as a response to stressful conditions in many bacterial species and has been ascribed to confer certain survival advantages. filamentation of campylobacter jejuni was demonstrated to occur spontaneously on entry in to stationary phase distinguishing it from many other bacteria where a reduction in size is more common. the aim of this study was to investigate the cues that give rise to filamentation of c. jejuni and c. col ...	2015	26175723
la35 poultry fecal marker persistence is correlated with that of indicators and pathogens in environmental waters.	disposal of fecally contaminated poultry litter by land application can deliver pathogens and fecal indicator bacteria (fib) into receiving waters via runoff. while water quality is regulated by fib enumeration, fib testing provides inadequate information about contamination source and health risk. this microbial source tracking (mst) study compared the persistence of the brevibacterium sp. strain la35 16s rrna gene (marker) for poultry litter with that of pathogens and fib under outdoor, enviro ...	2015	25934617
beyond campylobacter jejuni: understanding campylobacter coli infections in a systemic model of disease.		2015	26083465
diversity of the epsilonproteobacteria dsb (disulfide bond) systems.	the bacterial proteins of the dsb family-important components of the post-translational protein modification system-catalyze the formation of disulfide bridges, a process that is crucial for protein structure stabilization and activity. dsb systems play an essential role in the assembly of many virulence factors. recent rapid advances in global analysis of bacteria have thrown light on the enormous diversity among bacterial dsb systems. while the escherichia coli disulfide bond-forming system is ...	2015	26106374
heat shock-enhanced conjugation efficiency in standard campylobacter jejuni strains.	campylobacter jejuni, the leading bacterial cause of human gastroenteritis in the united states, displays significant strain diversity due to horizontal gene transfer. conjugation is an important horizontal gene transfer mechanism contributing to the evolution of bacterial pathogenesis and antimicrobial resistance. it has been observed that heat shock could increase transformation efficiency in some bacteria. in this study, the effect of heat shock on c. jejuni conjugation efficiency and the und ...	2015	25911489
towards fluorescence in vivo hybridization (fivh) detection of h. pylori in gastric mucosa using advanced lna probes.	in recent years, there have been several attempts to improve the diagnosis of infection caused by helicobacter pylori. fluorescence in situ hybridization (fish) is a commonly used technique to detect h. pylori infection but it requires biopsies from the stomach. thus, the development of an in vivo fish-based method (fivh) that directly detects and allows the visualization of the bacterium within the human body would significantly reduce the time of analysis, allowing the diagnosis to be performe ...	2015	25915865
microbial contamination of drinking water and human health from community water systems.	a relatively short list of reference viral, bacterial and protozoan pathogens appears adequate to assess microbial risks and inform a system-based management of drinking waters. nonetheless, there are data gaps, e.g. human enteric viruses resulting in endemic infection levels if poorly performing disinfection and/or distribution systems are used, and the risks from fungi. where disinfection is the only treatment and/or filtration is poor, cryptosporidiosis is the most likely enteric disease to b ...	2015	25821716
a community-based gastroenteritis outbreak after typhoon haiyan, leyte, philippines, 2013.	three weeks after typhoon haiyan, an increasing number of acute gastroenteritis cases were reported in kananga, leyte, an area where evacuated residents had returned home two days after the disaster. an outbreak investigation was conducted to identify the source and risk factors associated with the increase of gastroenteritis.	2015	25960917
prevalence of campylobacter species in milk and milk products, their virulence gene profile and anti-bio gram.	during the last decades, number of food poisoning cases due to campylobacter occurred, immensely. after poultry, raw milk acts as a second main source of campylobacter. therefore, the present study was undertaken to detect the prevalence of campylobacters in milk and milk products and to know the antibiotic sensitivity and virulence gene profile of campylobacter spp. in anand city, gujarat, india.	2015	27046986
[identification of zoonotic bacterial pathogens by the maldi tof ms method].	to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work.	2015	26312374
impact of oxidative stress defense on bacterial survival and morphological change in campylobacter jejuni under aerobic conditions.	campylobacter jejuni, a microaerophilic foodborne pathogen, inescapably faces high oxygen tension during its transmission to humans. thus, the ability of c. jejuni to survive under oxygen-rich conditions may significantly impact c. jejuni viability in food and food safety as well. in this study, we investigated the impact of oxidative stress resistance on the survival of c. jejuni under aerobic conditions by examining three mutants defective in key antioxidant genes, including ahpc, kata, and so ...	2015	25914692
multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis.	a wide range of enteric pathogens can cause infectious gastroenteritis. conventional diagnostic algorithms are time-consuming and often lack sensitivity and specificity. advances in molecular technology have provided new clinical diagnostic tools. multiplex polymerase chain reaction (pcr)-based testing has been used in gastroenterology diagnostics in recent years. this article presents a review of recent laboratory-developed multiplex pcr tests and current commercial multiplex gastrointestinal p ...	2015	26004652
first case report of campylobacter volucris bacteremia in an immunocompromised patient.	we report a case of campylobacter volucris bacteremia in an immunocompromised patient with polycythemia vera and alcoholic liver cirrhosis. to our knowledge, this is the first case report in which this organism has been isolated from a human clinical specimen.	2015	25832303
detection of thermophilic campylobacter sp. in raw chicken sausages by methods iso 10272: 2006 in curitiba - parana state - brazil.	the aim of this study was the detection of campylobacter sp. in raw chicken sausages using the methods iso 10272-1 and iso 10272-2. the overall prevalence of campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of con ...	2015	25763066
detection of multi-drug resistant escherichia coli in the urban waterways of milwaukee, wi.	urban waterways represent a natural reservoir of antibiotic resistance which may provide a source of transferable genetic elements to human commensal bacteria and pathogens. the objective of this study was to evaluate antibiotic resistance of escherichia coli isolated from the urban waterways of milwaukee, wi compared to those from milwaukee sewage and a clinical setting in milwaukee. antibiotics covering 10 different families were utilized to determine the phenotypic antibiotic resistance for a ...	2015	25972844
a prospective multi-center observational study of children hospitalized with diarrhea in ho chi minh city, vietnam.	we performed a prospective multicenter study to address the lack of data on the etiology, clinical and demographic features of hospitalized pediatric diarrhea in ho chi minh city (hcmc), vietnam. over 2,000 (1,419 symptomatic and 609 non-diarrheal control) children were enrolled in three hospitals over a 1-year period in 2009-2010. aiming to detect a panel of pathogens, we identified a known diarrheal pathogen in stool samples from 1,067/1,419 (75.2%) children with diarrhea and from 81/609 (13.3 ...	2015	25802437
comprehensive mapping of o-glycosylation in flagellin from campylobacter jejuni 11168: a multienzyme differential ion mobility mass spectrometry approach.	glycosylation of flagellin is essential for the virulence of campylobacter jejuni, a leading cause of bacterial gastroenteritis. here, we demonstrate comprehensive mapping of the o-glycosylation of flagellin from campylobacter jejuni 11168 by use of a bottom-up proteomics approach that incorporates differential ion mobility spectrometry (also known as high field asymmetric waveform ion mobility spectrometry or faims) together with proteolysis with proteinase k. proteinase k provides complementar ...	2015	25884275
narrow-spectrum inhibitors of campylobacter jejuni flagellar expression and growth.	campylobacter jejuni is a major cause of food-borne illness due to its ability to reside within the gastrointestinal tracts of chickens. multiple studies have identified the flagella of c. jejuni as a major determinant of chicken colonization. an inhibitor screen of approximately 147,000 small molecules was performed to identify compounds that are able to inhibit flagellar expression in a reporter strain of c. jejuni. several compounds that modestly inhibited motility of wild-type c. jejuni in s ...	2015	25870073
detection of thermophilic campylobacter sp. in raw chicken sausages by methods iso 10272: 2006 in curitiba - parana state - brazil.	the aim of this study was the detection of campylobacter sp. in raw chicken sausages using the methods iso 10272-1 and iso 10272-2. the overall prevalence of campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of con ...	2015	25763066
genome comparison provides molecular insights into the phylogeny of the reassigned new genus lysinibacillus.	lysinibacillus sphaericus (formerly named bacillus sphaericus) is incapable of polysaccharide utilization and some isolates produce active insecticidal proteins against mosquito larvae. its taxonomic status was changed to the genus lysinibacillus in 2007 with some other organisms previously regarded as members of bacillus. however, this classification is mainly based on physiology and phenotype and there is limited genomic information to support it.	2015	25888315
environmental monitoring of waterborne campylobacter: evaluation of the australian standard and a hybrid extraction-free mpn-pcr method.	campylobacter is the leading agent of diarrheal disease worldwide. this study evaluates a novel culture-pcr hybrid (mpn-pcr) assay for the rapid enumeration of campylobacter spp. from estuarine and wastewater systems. to first evaluate the current, culture-based, australian standard, an inter-laboratory study was conducted on 69 subsampled water samples. the proposed most-probable number (mpn)-pcr method was then evaluated, by analysing 147 estuarine samples collected over a 2 year period. data ...	2015	25709604
structural and functional implications of the interaction between macrolide antibiotics and bile acids.	macrolide antibiotics, such as azithromycin and erythromycin, are in widespread use for the treatment of bacterial infections. macrolides are taken up and excreted mainly by bile. additionally, they have been implicated in biliary system diseases and to modify the excretion of other drugs through bile. despite mounting evidence for the interplay between macrolide antibiotics and bile acids, the molecular details of this interaction remain unknown. herein, we show by nmr measurements that macroli ...	2015	25655041
comparison of eleven methods for genomic dna extraction suitable for large-scale whole-genome genotyping and long-term dna banking using blood samples.	over the recent years, next generation sequencing and microarray technologies have revolutionized scientific research with their applications to high-throughput analysis of biological systems. isolation of high quantities of pure, intact, double stranded, highly concentrated, not contaminated genomic dna is prerequisite for successful and reliable large scale genotyping analysis. high quantities of pure dna are also required for the creation of dna-banks. in the present study, eleven different d ...	2015	25635817
[detection of biofilm formation by selected pathogens relevant to the food industry].	detection of biofilm formation by microbial pathogens relevant to the food industry and comparison of biofilm formation under different conditions of culture.	2015	26448305
the polar and lateral flagella from plesiomonas shigelloides are glycosylated with legionaminic acid.	plesiomonas shigelloides is the unique member of the enterobacteriaceae family able to produce polar flagella when grow in liquid medium and lateral flagella when grown in solid or semisolid media. in this study on p. shigelloides 302-73 strain, we found two different gene clusters, one exclusively for the lateral flagella biosynthesis and the other one containing the biosynthetic polar flagella genes with additional putative glycosylation genes. p. shigelloides is the first enterobacteriaceae w ...	2015	26167161
effects of nitrate addition on rumen fermentation, bacterial biodiversity and abundance.	this study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with ion torrent sequencing and real-time polymerase chain reaction. three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0 ...	2015	26194220
core genome multilocus sequence typing scheme for high- resolution typing of enterococcus faecium.	enterococcus faecium, a common inhabitant of the human gut, has emerged in the last 2 decades as an important multidrug-resistant nosocomial pathogen. since the start of the 21st century, multilocus sequence typing (mlst) has been used to study the molecular epidemiology of e. faecium. however, due to the use of a small number of genes, the resolution of mlst is limited. whole-genome sequencing (wgs) now allows for high-resolution tracing of outbreaks, but current wgs-based approaches lack stand ...	2015	26400782
differentiation of campylobacter jejuni and campylobacter coli using multiplex-pcr and high resolution melt curve analysis.	campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. among campylobacter spp. campylobacter jejuni (c. jejuni) and c. coli are the most common causes of human infection. in this study, a multiplex pcr (mpcr) and high resolution melt (hrm) curve analysis were optimized for simultaneous detection and differentiation of c. jejuni and c. coli isolates. a segment of the hippuricase gene (hipo) of c. jejuni and putative aspartokinase (asp) gene of c. c ...	2015	26394042
milk modulates campylobacter invasion into caco-2 intestinal epithelial cells.	raw milk is a recognized source of campylobacter outbreaks, but pasteurization is an effective way to eliminate the causative agent of campylobacteriosis. whereas breastfeeding is protective against infectious diseases, consumption of formula milk is thought to be not. however, in relation to campylobacter, such data is currently unavailable. although both pasteurized and formula milk are pathogen free and prepared in a quality controlled manner, the effect they have on the virulence of campylob ...	2015	26495128
constitutive and inducible expression of the rrna methylase gene erm(b) in campylobacter.	macrolides are the antimicrobials of choice for treating human campylobacteriosis. the recent emergence of erm(b) in campylobacter bacteria threatens the utility of this class of antibiotics. here we report the constitutive and inducible expression of erm(b) in campylobacter isolates derived from diarrheal patients and food-producing animals. constitutive expression of erm(b) was associated with insertion and deletion in the regulatory region of the gene, providing the first documentation of the ...	2015	26259800
novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales.	the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ...	2015	27303676
novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales.	the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ...	2015	27303676
rapid host switching in generalist campylobacter strains erodes the signal for tracing human infections.	campylobacter jejuni and campylobacter coli are the biggest causes of bacterial gastroenteritis in the developed world, with human infections typically arising from zoonotic transmission associated with infected meat. because campylobacter is not thought to survive well outside the gut, host-associated populations are genetically isolated to varying degrees. therefore, the likely origin of most strains can be determined by host-associated variation in the genome. this is instructive for characte ...	2015	26305157
virulence characteristics of hcp (+) campylobacter jejuni and campylobacter coli isolates from retail chicken.	recently the type vi secretion system (t6ss), which can play a significant role in bacterial survival and pathogenesis, was reported in campylobacter spp., having the hcp gene as a key component.	2015	26207145
important role of a putative lytic transglycosylase cj0843c in β-lactam resistance in campylobacter jejuni.	beta-lactam antibiotics are an important class of antibiotics for treating bacterial infections. despite prevalent β-lactam resistance in campylobacter jejuni, the leading bacterial cause of human diarrhea in developed countries, molecular mechanism of β-lactam resistance in c. jejuni is still largely unknown. in this study, c. jejuni 81-176 was used for random transposon mutagenesis. screening of a 2,800-mutant library identified 22 mutants with increased susceptibility to ampicillin. of these ...	2015	26635760
antimicrobial resistance and genotypic diversity of campylobacter isolated from pigs, dairy, and beef cattle in tanzania.	foodborne campylobacter infections pose a serious threat to public health worldwide. however, the occurrence and characteristics of campylobacter in food animals and products remain largely unknown in tanzania. the objective of this study was to determine the prevalence, antibiotic resistance, and genetic profiles (sequence types, sts) of campylobacter isolated from feces of pigs and dairy and beef cattle in tanzania. overall, 259 (~30%) of 864 samples were positive for campylobacter spp, which ...	2015	26617582
abstracts.		2015	26553501
etiology of childhood infectious diarrhea in a developed region of china: compared to childhood diarrhea in a developing region and adult diarrhea in a developed region.	in china, great differences in economy, social characteristics and hygiene exist between developing and developed regions. a comparative study of infectious diarrhea between two regions was needed. three groups of diarrheal patients were collected: children ≤5 year-olds from beijing (developed region) and henan province (developing region), and adults over 18 year-olds from beijing. a questionnaire was used to survey and feces samples were examined for 16 enteropathogens. we enrolled 1422 childr ...	2015	26528820
mass spectrometry-based phyloproteomics (mspp): a novel microbial typing method.	maldi-tof-ms of microorganisms, which identifies microbes based on masses of high abundant low molecular weight proteins, is rapidly advancing to become another standard method in clinical routine laboratory diagnostics. allelic isoforms of these proteins result in varying masses of detectable biomarker ions. these variations give rise to a novel typing method for microorganisms named mass spectrometry-based phyloproteomics (mspp). the base of mspp is an amino acid sequence list of allelic isofo ...	2015	26303099
abstracts.		2015	26553501
survival in water of campylobacter jejuni strains isolated from the slaughterhouse.	campylobacter jejuni cause gastroenteritis in humans. the main transmission vector is the consumption or handling of contaminated chicken meat, since chicken can be colonized asymptomatically by c. jejuni. however, water has been implicated as the transmission vector in a few outbreaks. one possibility is the contamination of water effluent by c. jejuni originating from chicken farm. the ability of c. jejuni to be transmitted by water would be closely associated to its ability to survive in wate ...	2015	26702388
smrt sequencing of the campylobacter coli bfr-ca-9557 genome sequence reveals unique methylation motifs.	campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. in contrast to campylobacter jejuni, about 5 % of campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by dpni cutting specifically 5'-g(m)atc-3' motifs. this indicates significant differences in dna methylation between both microbial species. the goal of the study was to analyze the methylome of a c. coli strain susceptible to dpni digestion, to identify its meth ...	2015	26689587
molecular identification of campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being.	campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species. the aim of this study was to identify campylobacter jejuni and campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (pcr).	2015	27047055
a flagellar glycan-specific protein encoded by campylobacter phages inhibits host cell growth.	we previously characterized a carbohydrate binding protein, gp047, derived from lytic campylobacter phage nctc 12673, as a promising diagnostic tool for the identification of campylobacter jejuni and campylobacter coli. we also demonstrated that this protein binds specifically to acetamidino-modified pseudaminic acid residues on host flagella, but the role of this protein in the phage lifecycle remains unknown. here, we report that gp047 is capable of inhibiting c. jejuni growth both on solid an ...	2015	26694450
weakly deleterious mutations and low rates of recombination limit the impact of natural selection on bacterial genomes.	free-living bacteria are usually thought to have large effective population sizes, and so tiny selective differences can drive their evolution. however, because recombination is infrequent, "background selection" against slightly deleterious alleles should reduce the effective population size (ne) by orders of magnitude. for example, for a well-mixed population with 10(12) individuals and a typical level of homologous recombination (r/m = 3, i.e., nucleotide changes due to recombination [r] occu ...	2015	26670382
differential distribution of type ii crispr-cas systems in agricultural and nonagricultural campylobacter coli and campylobacter jejuni isolates correlates with lack of shared environments.	crispr (clustered regularly interspaced palindromic repeats)-cas (crispr-associated) systems are sequence-specific adaptive defenses against phages and plasmids which are widespread in prokaryotes. here we have studied whether phylogenetic relatedness or sharing of environmental niches affects the distribution and dissemination of type ii crispr-cas systems, first in 132 bacterial genomes from 15 phylogenetic classes, ranging from proteobacteria to actinobacteria. there was clustering of distinc ...	2015	26338188
detection of mycobacteria, mycobacterium avium subspecies, and mycobacterium tuberculosis complex by a novel tetraplex real-time pcr assay.	mycobacterium tuberculosis complex, mycobacterium avium, and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical and veterinary importance. considering the growing epidemiologic significance of wildlife-livestock-human interrelation, developing rapid detection tools of high specificity and sensitivity is vital to assess their presence and accelerate the process of diagnosing mycobacteriosis. here we describe the development and evaluation of a novel t ...	2015	25588660
the challenge of efflux-mediated antibiotic resistance in gram-negative bacteria.	the global emergence of multidrug-resistant gram-negative bacteria is a growing threat to antibiotic therapy. the chromosomally encoded drug efflux mechanisms that are ubiquitous in these bacteria greatly contribute to antibiotic resistance and present a major challenge for antibiotic development. multidrug pumps, particularly those represented by the clinically relevant acrab-tolc and mex pumps of the resistance-nodulation-division (rnd) superfamily, not only mediate intrinsic and acquired mult ...	2015	25788514
bacterial flagella: twist and stick, or dodge across the kingdoms.	the flagellum organelle is an intricate multiprotein assembly best known for its rotational propulsion of bacteria. however, recent studies have expanded our knowledge of other functions in pathogenic contexts, particularly adherence and immune modulation, e.g., for salmonella enterica, campylobacter jejuni, pseudomonas aeruginosa, and escherichia coli. flagella-mediated adherence is important in host colonisation for several plant and animal pathogens, but the specific interactions that promote ...	2015	25590430
functional metagenomics of spacecraft assembly cleanrooms: presence of virulence factors associated with human pathogens.	strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. we wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. three geographically distinct cleanrooms were sampled during the ...	2016	27667984
multidrug-resistant campylobacter coli in men who have sex with men, quebec, canada, 2015.		2016	27533504
accuracy of commercial kits and published primer pairs for the detection of periodontopathogens.	despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. the aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens.	2016	27020914
evaluation of a protective effect of in ovo delivered campylobacter jejuni omvs.	campylobacter jejuni is the most prevalent cause of a food-borne gastroenteritis in the developed world, with poultry being the main source of infection. campylobacter jejuni, like other gram-negative bacteria, constitutively releases outer membrane vesicles (omvs). omvs are highly immunogenic, can be taken up by mammalian cells, and are easily modifiable by recombinant engineering. we have tested their usefulness for an oral (in ovo) vaccination of chickens. four groups of 18-day-old chicken em ...	2016	27383607
what makes a bacterial species pathogenic?:comparative genomic analysis of the genus leptospira.	leptospirosis, caused by spirochetes of the genus leptospira, is a globally widespread, neglected and emerging zoonotic disease. while whole genome analysis of individual pathogenic, intermediately pathogenic and saprophytic leptospira species has been reported, comprehensive cross-species genomic comparison of all known species of infectious and non-infectious leptospira, with the goal of identifying genes related to pathogenesis and mammalian host adaptation, remains a key gap in the field. in ...	2016	26890609
monitoring of virulence genes, drug-resistance in campylobacter coli isolated from golden retrievers.	the investigation was performed on 75 of golden retriever puppies. faecal samples were collected on the 42 day of the puppies life (control). probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppies life). all isolates of campylobacter coli isolated prior to the administration of the probiotic were found to contain the cadf gene responsible for adhesion, as well as, the flaa ...	2016	28520333
high rates of contamination of poultry meat products with drug resistant campylobacter in metro manila, philippines.	a total of 265 chicken parts were collected from 15 wet markets and 15 supermarkets in metro manila, philippines. campylobacter spp. was isolated on modified charcoal cefoperazone deoxycholate agar plates and identified through biochemical tests and pcr amplification of genus and species specific genes. antimicrobial resistance profiles were determined following clinical and laboratory standards institute protocols. two hundred seven (78%) campylobacter spp. were isolated. c. jejuni and c. coli ...	2016	27795478
optimization of quantitative pcr methods for enteropathogen detection.	detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficiency. first, we evaluate the molecular detection and quantification of pathogens in rectal swabs versus stool, using paired flocked rectal swabs and whole stool collected from 129 children hospitalized ...	2016	27336160
comparative genomics of campylobacter fetus from reptiles and mammals reveals divergent evolution in host-associated lineages.	campylobacter fetus currently comprises three recognized subspecies, which display distinct host association. campylobacter fetus subsp. fetus and c fetus subsp. venerealis are both associated with endothermic mammals, primarily ruminants, whereas c fetus subsp. testudinum is primarily associated with ectothermic reptiles. both c. fetus subsp. testudinum and c. fetus subsp. fetus have been associated with severe infections, often with a systemic component, in immunocompromised humans. to study t ...	2016	27333878
potential use of fosfomycin-tromethamine for treatment of recurrent campylobacter species enteritis.	we report 2 cases of recurrent campylobacter coli enteritis caused by macrolide- and fluoroquinolone-resistant strains in 2 patients with hypogammaglobulinemia, successfully treated with a prolonged course of fosfomycin-tromethamine with no side effects. fosfomycin-tromethamine may be a feasible alternative therapy for recurrent enteritis caused by campylobacter species resistant to first-line drugs.	2016	27161640
complete genome sequences of multidrug-resistant campylobacter jejuni strain 14980a (turkey feces) and campylobacter coli strain 14983a (housefly from a turkey farm), harboring a novel gentamicin resistance mobile element.	multidrug resistance (mdr) in foodborne pathogens is a major food safety and public health issue. here we describe whole-genome sequences of two mdr strains of campylobacter jejuni and campylobacter coli from turkey feces and a housefly from a turkey farm. both strains harbor a novel chromosomal gentamicin resistance mobile element.	2016	27795285
epidemiology and rapd-pcr typing of thermophilic campylobacters from children under five years and chickens in morogoro municipality, tanzania.	campylobacter species are gram negative and flagellated bacteria under the genus campylobacter, family campylobacteriaceae. these pathogens cause zoonotic infections among human and animal populations. this study was undertaken between december 2006 and may 2007 to determine prevalence, risk factors and genetic diversity of thermophilic campylobacter isolates from children less than 5 years and chickens in morogoro municipality, tanzania.	2016	27871251
quantification of growth of campylobacter and extended spectrum β-lactamase producing bacteria sheds light on black box of enrichment procedures.	campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide, and is routinely found in meat originating from poultry, sheep, pigs, and cattle. effective monitoring of campylobacter contamination is dependent on the availability of reliable detection methods. the method of the international organization for standardization for the detection of campylobacter spp. in food (iso 10272-1:2006) recommends the use of bolton broth (bb) as selective enrichment ...	2016	27672384
cross-talk between the aeromonas hydrophila type iii secretion system and lateral flagella system.	aeromonas hydrophila is responsible for aeromonad septicaemia in fish, and gastroenteritis and wound infections in humans. the type iii secretion system (t3ss) is utilized by aeromonads to inject protein effectors directly into host cells. one of the major genetic regulators of the t3ss in several bacterial species is the arac-like protein exsa. previous studies have suggested a link between t3ss regulation and lateral flagella expression. the aim of this study was to determine the genetic regul ...	2016	27656180
polyphosphate and associated enzymes as global regulators of stress response and virulence in campylobacter jejuni.	campylobacter jejuni (c. jejuni), a gram-negative microaerophilic bacterium, is a predominant cause of bacterial foodborne gastroenteritis in humans worldwide. despite its importance as a major foodborne pathogen, our understanding of the molecular mechanisms underlying c. jejuni stress survival and pathogenesis is limited. inorganic polyphosphate (poly p) has been shown to play significant roles in bacterial resistance to stress and virulence in many pathogenic bacteria. c. jejuni contains the ...	2016	27672264
point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of campylobacter jejuni.	infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. campylobacter jejuni is an important zoonotic pathogen transmitted to humans mainly via the foodborne route. c. jejuni typically colonizes the gut, but a hypervirulent and rapidly expanding clone of c. jejuni recently emerged, ...	2016	27601641
association of campylobacter jejuni ssp. jejuni chemotaxis receptor genes with multilocus sequence types and source of isolation.	campylobacter jejuni's flagellar locomotion is controlled by eleven chemoreceptors. assessment of the distribution of the relevant chemoreceptor genes in the c. jejuni genomes deposited in the national center for biotechnology information (ncbi) database led to the identification of two previously unknown tlp genes and a tlp5 pseudogene. these two chemoreceptor genes share the same locus in the c. jejuni genome with tlp4 and tlp11, but the gene region encoding the periplasmic ligand binding doma ...	2016	27766165
evaluation of various campylobacter-specific quantitative pcr (qpcr) assays for detection and enumeration of campylobacteraceae in irrigation water and wastewater via a miniaturized most-probable-number-qpcr assay.	campylobacter spp. are the leading cause of bacterial gastroenteritis worldwide, and water is increasingly seen as a risk factor in transmission. here we describe a most-probable-number (mpn)-quantitative pcr (qpcr) assay in which water samples are centrifuged and aliquoted into microtiter plates and the bacteria are enumerated by qpcr. we observed that commonly used campylobacter molecular assays produced vastly different detection rates. in irrigation water samples, detection rates varied depe ...	2016	27235434
the risk of some veterinary antimicrobial agents on public health associated with antimicrobial resistance and their molecular basis.	the risk of antimicrobial agents used in food-producing animals on public health associated with antimicrobial resistance continues to be a current topic of discussion as related to animal and human public health. in the present review, resistance monitoring data, and risk assessment results of some important antimicrobial agents were cited to elucidate the possible association of antimicrobial use in food animals and antimicrobial resistance in humans. from the selected examples, it was apparen ...	2016	27803693
prevalence and antimicrobial resistance of campylobacter isolated from dressed beef carcasses and raw milk in tanzania.	campylobacter species are commonly transmitted to humans through consumption of contaminated foods such as milk and meat. the aim of this study was to investigate the prevalence, antimicrobial resistance, and genetic determinants of resistance of campylobacter isolated from raw milk and beef carcasses in tanzania. the antimicrobial resistance genes tested included blaoxa-61 (ampicillin), aph-3-1 (aminoglycoside), tet(o) (tetracycline), and cmeb (multi-drug efflux pump). the prevalence of campylo ...	2016	26153978
fate of viable but non-culturable listeria monocytogenes in pig manure microcosms.	the fate of two strains of listeria monocytogenes and their ability to become viable but non-culturable (vbnc) was investigated in microcosms containing piggery eﬄuents (two raw manures and two biologically treated manures) stored for 2 months at 8 and 20°c. levels of l. monocytogenes were estimated using the culture method, qpcr, and propidium monoazide treatment combined with qpcr (qpcrpma). the chemical composition and the microbial community structure of the manures were also analyzed. the s ...	2016	26973623
immune responses to mycobacterial heat shock protein 70 accompany self-reactivity to human bip in rheumatoid arthritis.	rheumatoid arthritis (ra) is an autoimmune disease, and a member of human heat shock protein (hsp) 70 protein family, binding immunoglobulin protein (bip), has been identified as an important autoantigen for t and b cells. we herein focused on mycobacterial (myc) hsps and immune responses to mychsps in ra patients. serum titers of antibodies against mychsp70 were significantly elevated in ra patients and correlated with serum anti-bip antibody titers. a mychsp70-derived hla-dr4 major epitope was ...	2016	26927756
genome sequencing and annotation of a campylobacter coli strain isolated from milk with multidrug resistance.	as the most prevalent bacterial cause of human gastroenteritis, food-borne campylobacter infections pose a serious threat to public health. whole genome sequencing (wgs) is a tool providing quick and inexpensive approaches for analysis of food-borne pathogen epidemics. here we report the wgs and annotation of a campylobacter coli strain, fnw20g12, which was isolated from milk in the united states in 1997 and carries multidrug resistance. the draft genome of fnw20g12 (ddbj/ena/genbank accession n ...	2016	27257607
detection of campylobacter jejuni in rectal swab samples from rousettus amplexicaudatus in the philippines.	bats are the second diversity species of mammals and widely distributed in the world. they are thought to be reservoir and vectors of zoonotic pathogens. however, there is scarce report of the evidence of pathogenic bacteria kept in bats. the precise knowledge of the pathogenic bacteria in bat microbiota is important for zoonosis control. thus, metagenomic analysis targeting the v3-v4 region of the 16s rrna of the rectal microbiota in rousettus amplexicaudatus was performed using high throughput ...	2016	27109214
application of alternative nucleic acid extraction protocols to progastro sscs assay for detection of bacterial enteric pathogens.	as an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. progastro ssc and shiga toxin-producing escherichia coli (i.e., stec) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column met ...	2016	26935731
whole-genome sequences of two campylobacter coli isolates from the antimicrobial resistance monitoring program in colombia.	campylobacter coli, along with campylobacter jejuni, is a major agent of gastroenteritis and acute enterocolitis in humans. we report the whole-genome sequences of two multidrug-resistance c. coli strains, isolated from the colombian poultry chain. the isolates contain a variety of antimicrobial resistance genes for aminoglycosides, lincosamides, fluoroquinolones, and tetracycline.	2016	26988048
whole-genome sequence of multidrug-resistant campylobacter coli strain col b1-266, isolated from the colombian poultry chain.	campylobacter coli is considered one of the main causes of food-borne illness worldwide. we report here the whole-genome sequence of multidrug-resistant campylobacter coli strain col b1-266, isolated from the colombian poultry chain. the genome sequences encode genes for a variety of antimicrobial resistance genes, including aminoglycosides, β-lactams, lincosamides, fluoroquinolones, and tetracyclines.	2016	26988047
protein chips for detection of salmonella spp. from enrichment culture.	food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. a pre-enrichment culture followed by selective agar plating are standard detection methods. molecular methods such as qpcr have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. biosensors also may provide a rapid tool to individuate a source of salmonella contamination at early times of pre-enrichment culture. forty ml of ...	2016	27110786
large sequence diversity within the biosynthesis locus and common biochemical features of campylobacter coli lipooligosaccharides.	despite the importance of lipooligosaccharides (loss) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of los in campylobacter coli in this study, we investigated the distribution of los locus classes among a large collection of unrelated c. coli isolates sampled from several different host species. furthermore, we paired c. coli genomic information and los chemical composition for the first time to investigate possible associations between l ...	2016	27481928
influence of enrichment and isolation media on the detection of campylobacter spp. in naturally contaminated chicken samples.	investigating campylobacter epidemiology requires adequate technique and media to ensure optimal culturing and accurate detection and isolation of campylobacter strains. in the present study, we investigated the performances of three enrichment durations in bolton broth (0, 24 and 48h) and compared four isolation media (mccda, karmali, butzler no. 2 and campyfood agar (cfa)) for the detection of campylobacter positive samples and the identification of campylobacter species, from naturally contam ...	2016	27373751
isolation of campylobacter spp. from client-owned dogs and cats, and retail raw meat pet food in the manawatu, new zealand.	campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. the disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. the prevalence of campylobacter spp. in dogs and cats varies widely, and data on new zealand pets are limited. this study aimed to investigate the prevalence of campylobacter spp. in dogs, cats and retail raw meat pet food ...	2016	27860343
genotyping and antibiotic resistance of thermophilic campylobacter isolated from chicken and pig meat in vietnam.	campylobacter species are recognized as the most common cause of foodborne bacterial gastroenteritis in humans. in this study nine campylobacter strains isolated from chicken meat and pork in hanoi, vietnam, were characterized using molecular methods and tested for antibiotic resistance.	2016	27175218