Publications

detection of thermophilic campylobacter from sparrows by multiplex pcr: the role of sparrows as a source of contamination of broilers with campylobacter.	the best combination of primers and the annealing temperature of multiplex pcr for campylobacter jejuni, campylobacter coli, and campylobacter lari were examined. the multiplex pcr was able to detect type strains of the three species. all results of identification of wild strains (30 strains of c. jejuni, 20 strains of c. coli, and 4 strains of c. lari) by the multiplex pcr coincided with those of the conventional biochemical identification tests, suggesting that the multiplex pcr can simultaneo ...	2000	11193345
national surveillance of campylobacter in broilers at slaughter in denmark in 1998.	a surveillance study for thermophilic campylobacter spp. in broiler flocks was carried out for the year 1998 in denmark. the study included examinations of 4286 broiler flocks comprising samples from 57,000 birds. overall, a flock prevalence of 46.0% was recorded. the species distribution was campylobacter jejuni 86%, campylobacter coli 11%, campylobacter lari 1%, other not further diagnosed species 2%. the prevalence was significantly higher in the period from june to october (3.2 < odds ratio ...	2000	11195660
cytolethal distending toxin sequence and activity in the enterohepatic pathogen helicobacter hepaticus.	little is known about the molecular pathogenesis of hepatitis and enterocolitis caused by enterohepatic helicobacter species. sonicates of the murine pathogen helicobacter hepaticus were found to cause progressive cell distension, accumulation of filamentous actin, and g(2)/m cell cycle arrest in hela cell monolayers. the genes encoding this cytotoxic activity were cloned from h. hepaticus. three open reading frames with closest homology to cdta, cdtb, and cdtc from campylobacter jejuni were ide ...	2000	10603386
localized reversible frameshift mutation in the flha gene confers phase variability to flagellin gene expression in campylobacter coli.	phase variation of flagellin gene expression in campylobacter coli ua585 was correlated with high-frequency, reversible insertion and deletion frameshift mutations in a short homopolymeric tract of thymine residues located in the n-terminal coding region of the flha gene. mutation-based phase variation in flha may generate functional diversity in the host and environment.	2000	10613882
prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters.	the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ...	2000	10618103
genotyping of campylobacter spp.		2000	10618195
isolation and characterization of campylobacter jejuni subsp. jejuni from macaroni penguins (eudyptes chrysolophus) in the subantarctic region.	on bird island, south georgia, albatrosses (n = 140), penguins (n = 100), and fur seals (n = 206) were sampled for campylobacter jejuni. c. jejuni subsp. jejuni was recovered from three macaroni penguins (eudyptes chrysolophus). these isolates, the first reported for the subantarctic region, showed low genetic diversity and high similarity to northern hemisphere c. jejuni isolates, possibly suggesting recent introduction to the area.	2000	10618265
virulent strains of helicobacter pylori demonstrate delayed phagocytosis and stimulate homotypic phagosome fusion in macrophages.	helicobacter pylori colonizes the gastric epithelium of approximately 50% of the world's population and plays a causative role in the development of gastric and duodenal ulcers. h. pylori is phagocytosed by mononuclear phagocytes, but the internalized bacteria are not killed and the reasons for this host defense defect are unclear. we now show using immunofluorescence and electron microscopy that h. pylori employs an unusual mechanism to avoid phagocytic killing: delayed entry followed by homoty ...	2000	10620610
antibiotic resistance in campylobacter strains isolated from animals, foods, and humans in spain in 1997-1998.	colonization by campylobacter strains was investigated in human, broiler, and pig fecal samples from 1997-1998, as well as in foods of animal origin, and antibiotic susceptibility testing was carried out for these strains. campylobacter strains were isolated in the foods of animal origin (55 of 101 samples; 54.4%), intestinal samples from broilers (85 of 105; 81%), and pigs (40 of 45; 88.9%). a total of 641 campylobacter strains were isolated from 8,636 human fecal samples of clinical origin (7. ...	2000	10639348
multiplex pcr: optimization and application in diagnostic virology.	pcr has revolutionized the field of infectious disease diagnosis. to overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex pcr, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed. multiplex pcrs to detect viral, bacterial, and/or other infectious agents in one reaction tube have been described. early studies highlighted the obstacles that can jeopardize the production ...	2000	11023957
amplified-fragment length polymorphism analysis: the state of an art.		1999	10488158
nomenclature for new tetracycline resistance determinants.	letters of the english alphabet have heretofore been used to name tetracycline resistance determinants. since all 26 letters have now been used, a nomenclature employing numerals is recommended for future determinants, and one laboratory has offered to coordinate the assignment of numerals.	1999	10348788
rapid detection of the chlamydiaceae and other families in the order chlamydiales: three pcr tests.	few identification methods will rapidly or specifically detect all bacteria in the order chlamydiales, family chlamydiaceae. in this study, three pcr tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. two tests exclusively recognized the chlamydiaceae: a multiplex test targeting the ompa gene and the rrna intergenic spacer and a taqman test targeting the 23s ribosomal dna. the multiplex test was able to detect as few as 200 inclusion ...	1999	9986815
antiseptics and disinfectants: activity, action, and resistance.	antiseptics and disinfectants are extensively used in hospitals and other health care settings for a variety of topical and hard-surface applications. a wide variety of active chemical agents (biocides) are found in these products, many of which have been used for hundreds of years, including alcohols, phenols, iodine, and chlorine. most of these active agents demonstrate broad-spectrum antimicrobial activity; however, little is known about the mode of action of these agents in comparison to ant ...	1999	9880479
drug resistance in campylobacter jejuni, c coli, and c lari isolated from humans in north west england and wales, 1997.	to test the sensitivity of strains of campylobacter species isolated from humans in england and wales against a range of antimicrobial agents for the purpose of monitoring therapeutic efficacy and as an epidemiological marker.	1999	10690169
flagellin gene polymorphism analysis of campylobacter compared with antigen serotyping.	flagellin gene sequence polymorphisms were used to discriminate amongst 53 strains of campylobacter jejuni and c. coli. the campylobacter strains were made up of forty-three strains of campylobacter jejuni and 10 strains of campylobacter coli. the results were analysed in relation to penner serotyping. twenty dna pcr-rflp patterns (genotypes) were identified by analysis of dde i fragment length polymorphisms in flagellin gene (fla a and fla b) polymerase chain reaction (pcr) products. flagellin ...	1999	10627993
motility of helicobacter pylori is coordinately regulated by the transcriptional activator flgr, an ntrc homolog.	sigma54 is the subunit of bacterial rna polymerase that transcribes from promoters with enhancer elements bound by enhancer-binding proteins. by computer searches of helicobacter pylori genomic sequences, chromosomal gene disruption, and rna analyses, we have identified sigma54-recognized promoters that regulate transcription of flagellar basal body and hook genes, as well as the enhancer-binding protein flgr (flagellum regulator), a transactivating protein of the ntrc family. we demonstrate tha ...	1999	9882675
pcr-enzyme-linked immunosorbent assay for detection and identification of campylobacter species: application to isolates and stool samples.	we report a pcr-enzyme-linked immunosorbent assay which identifies campylobacter species by capture hybridization of a single-stranded 16s rrna gene amplicon with species-specific probes in a microtiter plate format. specificities were confirmed for both reference and field strains, but the type strain of campylobacter coli was atypical. the assay was rapid, informative, and usable with stool-extracted dna.	1999	9889235
identification of the enteropathogens campylobacter jejuni and campylobacter coli based on the cadf virulence gene and its product.	campylobacter jejuni and campylobacter coli are common causes of gastroenteritis in humans. infection with c. jejuni or c. coli is commonly acquired by eating undercooked chicken. the goal of this study was to develop specific detection assays for c. jejuni and c. coli isolates based on the cadf virulence gene and its product. the cadf gene from c. jejuni and c. coli encodes a 37-kda outer membrane protein that promotes the binding of these pathogens to intestinal epithelial cells. a fragment of ...	1999	9986804
characterization of human bactericidal antibodies to bordetella pertussis.	the bordetella pertussis brka protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. to further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to b. pertussis (infected as adults, occupational exposure, immunized with an acellular vaccine, or no identified exposure) against a wild-type strain and a brka complement-sensit ...	1999	10024590
inducing enterotoxigenic properties in campylobacter jejuni and campylobacter coli by serial intraperitoneal passage in mice.		1999	10029919
comparison of flagellin genes from clinical and environmental pseudomonas aeruginosa isolates.	pseudomonas aeruginosa, an important opportunistic pathogen, was isolated from environmental samples and compared to clinically derived strains. while p. aeruginosa was isolated readily from an experimental mushroom-growing unit, it was found only rarely in other environmental samples. a flagellin gene pcr-restriction fragment length polymorphism analysis of the isolates revealed that environmental and clinical p. aeruginosa strains are not readily distinguishable. the variation in the central r ...	1999	10049879
evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems.	the performances of five automated microbial identification systems, relative to that of a reference identification system, for their ability to accurately and repeatedly identify six common food-borne pathogens were assessed. the systems assessed were the microlog system (biolog inc., hayward, calif.), the microbial identification system (mis; midi inc., newark, del.), the vitek system (biomérieux vitek, hazelwood, mo.), the microscan walkaway 40 system (dade-microscan international, west sacra ...	1999	10074506
mutational analysis of the vaca promoter provides insight into gene transcription in helicobacter pylori.	analysis of 12 helicobacter pylori promoters indicates the existence of a consensus -10 hexamer (tataat) but little conservation of -35 sequences. in this study, mutations in either the h. pylori vaca -10 region or the -35 region resulted in decreased vaca transcription and suggested that an extended -10 motif is utilized. thus, despite the lack of a -35 consensus sequence for h. pylori promoters, the -35 region plays a functional role in vaca transcription.	1999	10094707
serotypes and typability of campylobacter jejuni and campylobacter coli isolated from poultry products.	campylobacter infection is one of the most common bacterial enteric pathogens. campylobacter jejuni and campylobacter coli infections are mostly food- and waterborne and especially poultry is often assumed to be an important source. the heat-stable serotyping system (the 'penner' scheme) was used to study the serotype distribution of c. jejuni and c. coli isolated from different food products of poultry origin sampled from retail outlets in denmark. a total of 156 isolates were serotyped, 85% of ...	1999	10100900
fluoroquinolone resistance in salmonellas and campylobacters from humans.		1999	10102879
detection of cytolethal distending toxin activity and cdt genes in campylobacter spp. isolated from chicken carcasses.	this study was designed to determine whether isolates from chicken carcasses, the primary source of campylobacter jejuni and campylobacter coli in human infections, commonly carry the cdt genes and also whether active cytolethal distending toxin (cdt) is produced by these isolates. campylobacter spp. were isolated from all 91 fresh chicken carcasses purchased from local supermarkets. campylobacter spp. were identified on the basis of both biochemical and pcr tests. of the 105 isolates, 70 (67%) ...	1999	10103243
detection of small numbers of campylobacter jejuni and campylobacter coli cells in environmental water, sewage, and food samples by a seminested pcr assay.	a rapid and sensitive assay was developed for detection of small numbers of campylobacter jejuni and campylobacter coli cells in environmental water, sewage, and food samples. water and sewage samples were filtered, and the filters were enriched overnight in a nonselective medium. the enrichment cultures were prepared for pcr by a rapid and simple procedure consisting of centrifugation, proteinase k treatment, and boiling. a seminested pcr based on specific amplification of the intergenic sequen ...	1999	10103261
cytolethal distending toxin genes in campylobacter jejuni and campylobacter coli isolates: detection and analysis by pcr.	campylobacter jejuni produces a toxin called cytolethal distending toxin (cdt). knowledge of the prevalence and homogeneity of campylobacter sp. cdt genes is incomplete. in this work, we identified four pcr primer pairs that collectively amplified cdt genes in all of the c. jejuni and campylobacter coli strains tested. restriction analyses of the cdt pcr products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the s ...	1999	10203548
differentiation of campylobacter coli and c. jejuni by length and dna sequence of the 16s-23s rrna internal spacer region.	the internal spacer region (isr) between the 16s and 23s rrna genes of campylobacter was investigated by pcr fragment length typing and dna sequencing of clinical and chicken wild-type isolates. pcr fragment length typing showed one fragment of 859 nt in length for the 12 strains of campylobacter coli investigated. thirty-six of the campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. three strains showed two fragments between 501 and 92 ...	1999	10206715
the use of hipo, encoding benzoylglycine amidohydrolase (hippuricase), as a reporter of gene expression in campylobacter coli.	a novel integrative promoter probe vector which utilizes the campylobacter jejuni hipo gene as a reporter of gene expression was developed as a genetic tool in campylobacter coli. the utility of the system was demonstrated by coupling expression of the hipo reporter to the promoters of flaa, flab and kata. subsequently, expression of these genes could be monitored accurately from chromosomally-borne transcriptional fusions using a simple non-destructive colorimetric assay. the system should serv ...	1999	10212441
survival times of campylobacter coli in sterilized buffalo milk.	the survival level of five campylobacter coli strains isolated from bovine faeces was studied in sterilized buffalo milk kept at 4 degrees c under aerobic conditions. all strains died between 21 and 30 h of storage.	1999	10216458
ferric enterobactin binding and utilization by neisseria gonorrhoeae.	feta, formerly designated frpb, an iron-regulated, 76-kda neisserial outer membrane protein, shows sequence homology to the tonb-dependent family of receptors that transport iron into gram-negative bacteria. although feta is commonly expressed by most neisserial strains and is a potential vaccine candidate for both neisseria gonorrhoeae and neisseria meningitidis, its function in cell physiology was previously undefined. we now report that feta functions as an enterobactin receptor. n. gonorrhoe ...	1999	10217784
high-resolution genomic fingerprinting of campylobacter jejuni and campylobacter coli by analysis of amplified fragment length polymorphisms.	a method for high-resolution genomic fingerprinting of the enteric pathogens campylobacter jejuni and campylobacter coli, based on the determination of amplified fragment length polymorphism, is described. the potential of this method for molecular epidemiological studies of these species is evaluated with 50 type, reference, and well-characterised field strains. amplified fragment length polymorphism fingerprints comprised over 60 bands detected in the size range 35-500 bp. groups of outbreak s ...	1999	10220884
cloning and characterization of polyphosphate kinase and exopolyphosphatase genes from pseudomonas aeruginosa 8830.	pseudomonas aeruginosa accumulates polyphosphates in response to nutrient limitations. to elucidate the function of polyphosphate in this microorganism, we have investigated polyphosphate metabolism by isolating from p. aeruginosa 8830 the genes encoding polyphosphate kinase (ppk) and exopolyphosphatase (ppx), which are involved in polyphosphate synthesis and degradation, respectively. the 690- and 506-amino-acid polypeptides encoded by the two genes have been expressed in escherichia coli and p ...	1999	10224002
stability of related human and chicken campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis.	the genomic stability of 12 campylobacter jejuni strains consisting of two groups of human and chicken isolates was studied by analysis of their pfge (pulsed-field gel electrophoresis) patterns after passage through newly hatched chicks' intestines. the patterns of smai, sali, and sacii digests remained stable after intestinal passage, except for those of two strains. one originally human strain, fb 6371, changed its genotype from ii/a (smai/sacii) to i/b. another strain, bti, originally isolate ...	1999	10224037
identification of virulence genes of helicobacter pylori by random insertion mutagenesis.	the complete genome of the gram-negative bacterial pathogen helicobacter pylori, an important etiological agent of gastroduodenal disease in humans, has recently been published. this sequence revealed that the putative products of roughly one-third of the open reading frames (orfs) have no significant homology to any known proteins. to be able to analyze the functions of all orfs, we constructed an integration plasmid for h. pylori and used it to generate a random mutant library in this organism ...	1999	10225906
study of the presence of campylobacter jejuni and c. coli in sand samples from four swiss chicken farms.	chicken farms are frequently infected with campylobacter jejuni and campylobacter coli. the objective of the present study was to investigate environmental samples from chicken farms for the presence of c. jejuni and c. coli. every week between july and november 1997, three sand samples from the runs of four chicken farms were analyzed by culture and directly by polymerase chain reaction (pcr). these two detection methods were compared to each other. a total of 231 samples were tested. eleven sa ...	1999	10229951
rapid identification of thermotolerant campylobacter jejuni, campylobacter coli, campylobacter lari, and campylobacter upsaliensis from various geographic locations by a gtpase-based pcr-reverse hybridization assay.	recently, a gene from campylobacter jejuni encoding a putative gtpase was identified. based on two semiconserved gtp-binding sites encoded within this gene, pcr primers were selected that allow amplification of a 153-bp fragment from c. jejuni, c. coli, c. lari, and c. upsaliensis. sequence analysis of these pcr products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant campylobacter species. multiple probes we ...	1999	10325325
differentiation of helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay.	plant and animal lectins with various carbohydrate specificities were used to type 35 irish clinical isolates of helicobacter pylori and the type strain nctc 11637 in a microtiter plate assay. initially, a panel of eight lectins with the indicated primary specificities were used: anguilla anguilla (aaa), lotus tetragonolobus (lotus a), and ulex europaeus i (uea i), specific for alpha-l-fucose; solanum tuberosum (sta) and triticum vulgaris (wga), specific for beta-n-acetylglucosamine; glycine max ...	1999	10325361
septic shock due to helicobacter fennelliae in a non-human immunodeficiency virus-infected heterosexual patient.	helicobacter fennelliae (formerly campylobacter fennelliae) has been reported to cause bacteremia in homosexual men with or without human immunodeficiency virus (hiv) infection. we report here a 48-year-old, non-hiv-infected, heterosexual man with diabetes mellitus and cirrhosis of the liver who developed bacteremia and septic shock due to h. fennelliae. the patient was treated successfully initially with intravenous ampicillin-sulbactam and ceftazidime, followed by ampicillin-sulbactam only. th ...	1999	10325388
passive immunity against human pathogens using bovine antibodies.		1999	10337007
biotin-streptavidin enzyme-linked immunosorbent assay for the detection of antibodies to campylobacter jejuni and c. coli in chickens.	an enzyme-linked immunosorbent assay (elisa) was developed in a homologous system with bacterial ultrasonic-treated proteins as the antigen and antisera from chickens infected orally and subcutaneously with the strain campylobacter jejuni serovar 6 (cj 6). the cut-off level was determined using antisera from non-infected specific-pathogen-free chickens up to the age of 10 weeks. the suitability of the elisa system was verified using antisera taken from chickens orally infected at the age of 4 we ...	1999	10337238
high-resolution genotyping of campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting.	for epidemiological studies of campylobacter infections, molecular typing methods that can differentiate campylobacters at the strain level are needed. in this study we used a recently developed genotyping method, amplified fragment length polymorphism (aflp), which is based on selective amplification of restriction fragments of chromosomal dna, for genetic typing of campylobacter jejuni and campylobacter coli strains derived from humans and poultry. we developed an automated aflp fingerprinting ...	1999	10347015
generation of a superoxide dismutase (sod)-deficient mutant of campylobacter coli: evidence for the significance of sod in campylobacter survival and colonization.	the microaerophilic nature of campylobacter species implies an inherent sensitivity towards oxygen and its reduction products, particularly the superoxide anion. the deleterious effects of exposure to superoxide radicals are counteracted by the activity of superoxide dismutase (sod). we have shown previously that campylobacter coli possesses an iron cofactored sod. the sodb gene of c. coli ua585 was insertionally inactivated by the site-specific insertion of a teto cassette. organisms harboring ...	1999	10347040
evaluation of the anaeropack campylo system for growth of microaerophilic bacteria.	growth of microaerophilic bacteria in the anaeropack campylo (mitsubishi gas chemical america, inc., new york, n.y.) atmosphere generation system was compared to growth in the campypak plus jar and campypak pouch (becton-dickinson microbiology systems [bdms], cockeysville, md.). growth in the anaeropack campylo system was considered equivalent to or better than growth obtained in the campypak plus and campypak pouch systems for 48 of the 50 helicobacter pylori strains and for all 28 campylobacte ...	1999	10364622
campylobacter coli strains with enlarged flagellin genes isolated from river water.	a group of campylobacters isolated from river water were found to possess unusually large flagellin genes. both phenotype and serology were consistent with identification as campylobacter coli. phylogenetic analysis of small (16s, rrs) and large subunit (23s, rrl) rrna genes of a representative strain, nctc 13006, demonstrated high levels of relatedness with c. jejuni and c. coli (99.1 and 98.3% similarity for 16s; 99.3 and 99.4% similarity for 23s). large flagellin proteins were demonstrated by ...	1999	10376486
in-vitro activity of moxifloxacin against fluoroquinolone-resistant strains of aerobic gram-negative bacilli and enterococcus faecalis.	mics of the new fluoroquinolone, moxifloxacin, and those of ciprofloxacin, ofloxacin and sparfloxacin for 19 genetically characterized fluoroquinolone-resistant strains were determined by the agar dilution method. the mics of moxifloxacin for escherichia isolates with one mutation in gyra (corresponding to ser83-->leu or asp87-->gly substitution) were 0.25-0.5 mg/l, while those of ciprofloxacin, ofloxacin and sparfloxacin were 0.06-0.25, 1 and 0.12-0.5 mg/l, respectively. these values were four- ...	1999	10382871
evaluation of the effect of temperature and nutrients on the survival of campylobacter spp. in water microcosms.	batch microcosms containing various water types (de-ionized and river water with or without sediment), incubated at a range of temperatures (5-37 degrees c), were used to facilitate a comparative evaluation of the significance of such variables and their interactions upon the collective and individual survival of four species of thermophilic campylobacter. all variables significantly influenced (p < = 0.031) population decay rates. minimal decay for the group was identified at low temperatures ( ...	1999	10389249
rapid detection, by pcr and reverse hybridization, of mutations in the helicobacter pylori 23s rrna gene, associated with macrolide resistance.	a pcr-based reverse hybridization system (research prototype kit inno-lipa for h. pylori resistance) was developed and evaluated for simultaneous detection of 23s ribosomal dna point mutations, associated with macrolide resistance in helicobacter pylori. fifty-seven h. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by pcr-lipa (detecting mutations a2115-->g, g2141-->a, a2142-->g, a2142-->c, a2143-->g, a2143-->c, and a2143-->t), d ...	1999	10390244
identification of a glycoprotein produced by enterotoxigenic escherichia coli.	enterotoxigenic escherichia coli (etec) strain h10407 is capable of invading epithelial cell lines derived from the human ileocecum and colon in vitro. two separate chromosomally encoded invasion loci (tia and tib) have been cloned from this strain. these loci direct nonadherent and noninvasive laboratory strains of e. coli to adhere to and invade cultured human intestinal epithelial cells. the tib locus directs the synthesis of tiba, a 104-kda outer membrane protein that is directly correlated ...	1999	10417177
campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of human intestinal cells.	campylobacter jejuni uptake into cultured int407 cells was analyzed kinetically over a wide range of starting multiplicities of infection (moi; from 0.02 to 20,000 bacteria/epithelial cell). the efficiency of internalization was the highest at moi of 0.02 and decreased steadily at higher mois, presumably due to reported c. jejuni autoagglutination at higher densities. total internalized campylobacter cfu increased gradually from an moi of 0.02 to a peak at an moi of 200 (reaching an average of t ...	1999	10417189
occurrence, removal and seasonal variation of thermophilic campylobacters and arcobacter in sewage sludge.	the presence of thermophilic campylobacters and arcobacter was investigated in four types of sewage sludge taken from the treatment plant in bologna (italy): primary, activated, thickened and anaerobically digested sludge. campylobacter jejuni and campylobacter coli were more numerous during the march-september period and were found only in primary sludge (22.7%) with mean counts of 278 mpn/g dry matter and 1403 mpn/g dry matter respectively. arcobacter butzleri were found in all types of sludge ...	1999	10418097
incidence of salmonella, campylobacter jejuni, campylobacter coli, and listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the belgian retail market.	from january 1997 to may 1998, 772 samples of poultry carcasses and poultry products for sale on the retail market in belgium were analyzed for the presence of salmonella spp., salmonella enteritidis, campylobacter jejuni, c. coli, and listeria monocytogenes per 100 cm2 or 25 g. poultry samples were contaminated with salmonella (36.5%), c. jejuni and c. coli (28.5%), and l. monocytogenes (38.2%). in about 12.3% of the poultry samples, the l. monocytogenes contamination level exceeded 1 cfu per g ...	1999	10419264
an iron-regulated alkyl hydroperoxide reductase (ahpc) confers aerotolerance and oxidative stress resistance to the microaerophilic pathogen campylobacter jejuni.	microaerophiles like campylobacter jejuni must resist oxidative stresses during transmission or infection. growth of c. jejuni 81116 under iron limitation greatly increased the expression of two polypeptides of 26 and 55 kda. the identification of these proteins by n-terminal amino acid sequencing showed both to be involved in the defense against oxidative stress. the 55-kda polypeptide was identical to c. jejuni catalase (kata), whereas the n terminus of the 26-kda polypeptide was homologous to ...	1999	10438747
direct analysis and identification of helicobacter and campylobacter species by maldi-tof mass spectrometry.	campylobacter jejuni, campylobacter fetus, and campylobacter coli were compared with helicobacter pylori and helicobacter mustelae by direct analysis of individual cultured colonies in 50% methanol-water with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (maldi-tof ms). h. pylori and campylobacter species from blood agar culture produced unique, complex spectra with over 25 different ions in mass/charge (m/z) range from 2,000 to 62,000. a biomarker for h. pylori ...	1999	10464475
sulfonamide resistance in clinical isolates of campylobacter jejuni: mutational changes in the chromosomal dihydropteroate synthase.	the characterization of the genetic basis of sulfonamide resistance in campylobacter jejuni was attempted. the resistance determinant from a sulfonamide-resistant strain of c. jejuni was cloned and was found to show 42% identity with the folp gene (which codes for dihydropteroate synthase, the target of sulfonamides) of the related bacterium helicobacter pylori. the sequences of the areas surrounding the folp gene in c. jejuni showed similarity to those of the areas surrounding the corresponding ...	1999	10471557
metabolism and genetics of helicobacter pylori: the genome era.	the publication of the complete sequence of helicobacter pylori 26695 in 1997 and more recently that of strain j99 has provided new insight into the biology of this organism. in this review, we attempt to analyze and interpret the information provided by sequence annotations and to compare these data with those provided by experimental analyses. after a brief description of the general features of the genomes of the two sequenced strains, the principal metabolic pathways are analyzed. in particu ...	1999	10477311
hp0333, a member of the dpra family, is involved in natural transformation in helicobacter pylori.	helicobacter pylori is naturally competent for dna transformation, but the mechanism by which transformation occurs is not known. for haemophilus influenzae, dpra is required for transformation by chromosomal but not plasmid dna, and the complete genomic sequence of h. pylori 26695 revealed a dpra homolog (hp0333). examination of genetic databases indicates that dpra homologs are present in a wide variety of bacterial species. to examine whether hp0333 has a function similar to dpra of h. influe ...	1999	10482496
the c-terminal domain of the bordetella pertussis autotransporter brka forms a pore in lipid bilayer membranes.	brka is a 103-kda outer membrane protein of bordetella pertussis that mediates resistance to antibody-dependent killing by complement. it is proteolytically processed into a 73-kda n-terminal domain and a 30-kda c-terminal domain as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. brka is also a member of the autotransporter family of proteins. translocation of the n-terminal domain of the protein across the outer membrane is hypothesized to occur through a pore formed by ...	1999	10482528
recombinant single-chain fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization.	using phage-display technology, a recombinant single-chain fv antibody fragment (scfv) was rapidly generated from the k16-16 hybridoma secreting mouse monoclonal antibody (mab) that binds to acetylaminofluorene-labeled dna (aaf-dna). the selected a4 phage-scfv specifically bound to aaf-dna. the anti-aaf scfv gene was then recloned into a fusion vector for the production of a hybrid protein comprising the antibody fragment fused to a potent bacterial alkaline phosphatase variant (phoav). the anti ...	1999	10485265
phage typing of campylobacter jejuni and campylobacter coli and its use as an adjunct to serotyping.	campylobacter is the most commonly reported cause of gastro-intestinal infection in england and wales, with over 50,000 reported cases in 1997. the majority of human campylobacter isolates in england and wales are c. jejuni (c. 90%) with most of the remainder being c. coli. we describe the use of phage typing as an extension to serotyping for more detailed characterization within these two species. the scheme was piloted during a study of 2407 c. jejuni and 182 c. coli strains isolated in wales ...	1999	10487641
ciprofloxacin resistance in campylobacter jejuni isolates: detection of gyra resistance mutations by mismatch amplification mutation assay pcr and dna sequence analysis.	the gyra quinolone resistance determining region was sequenced from 13 ciprofloxacin-resistant and 20 ciprofloxacin-susceptible campylobacter jejuni isolates. all isolates resistant to ciprofloxacin had thr-86-to-ile mutations, a mutation frequently associated with the acquisition of resistance to fluoroquinolones. a mismatch amplification mutation assay (mama) pcr protocol was developed that detects this gyra mutation in quinolone-resistant isolates. the mama pcr provides a means for routine de ...	1999	10488192
specific pcr identification and differentiation of the thermophilic campylobacters, campylobacter jejuni, c. coli, c. lari, and c. upsaliensis.	a sensitive pcr assay that detects the thermophilic campylobacters c. jejuni, c. coli, c. lari, and c. upsaliensis is reported. furthermore, by digestion of the pcr products with two restriction enzymes, species differentiation was demonstrated. thus, the present method has the potential to be used for both detection and identification of thermophilic campylobacter species.	1999	10488210
the absence of cecal colonization of chicks by a mutant of campylobacter jejuni not expressing bacterial fibronectin-binding protein.	campylobacter jejuni is a common cause of human gastrointestinal illness throughout the world. infections with c. jejuni and campylobacter coli are frequently acquired by eating undercooked chicken. the ability of c. jejuni to become established in the gastrointestinal tract of chickens is believed to involve binding of the bacterium to the gastrointestinal surface. a 37-kd outer membrane protein, termed cadf, has been described that facilitates the binding of campylobacter to fibronectin. this ...	1999	10494431
phase variation in helicobacter pylori lipopolysaccharide due to changes in the lengths of poly(c) tracts in alpha3-fucosyltransferase genes.	the lipopolysaccharide (lps) of helicobacter pylori expresses the lewis x (lex) and/or ley antigen. we have shown previously that h. pylori lps displays phase variation whereby an lex-positive strain yields variants with different lps serotypes, for example, lex plus ley or nonfucosylated polylactosamine. h. pylori has two alpha3-fucosyltransferase genes that both contain poly(c) tracts. we now demonstrate that these tracts can shorten or lengthen randomly, which results in reversible frameshift ...	1999	10496917
viability and dna maintenance in nonculturable spiral campylobacter jejuni cells after long-term exposure to low temperatures.	survival of campylobacter jejuni at 4 and 20 degrees c was investigated by using cellular integrity, respiratory activity, two-dimensional (2d) protein profile, and intact dna content as indicators of potential viability of nonculturable cells. intact dna content after 116 days, along with cellular integrity and respiring cells, was detected for up to 7 months at 4 degrees c by pulsed-field gel electrophoresis. most changes in 2d protein profiles involved up- or down-regulation.	1999	10508106
campylobacter jejuni contains two fur homologs: characterization of iron-responsive regulation of peroxide stress defense genes by the perr repressor.	expression of the peroxide stress genes alkyl hydroperoxide reductase (ahpc) and catalase (kata) of the microaerophile campylobacter jejuni is repressed by iron. whereas iron repression in gram-negative bacteria is usually carried out by the fur protein, previous work showed that this is not the case in c. jejuni, as these genes are still iron repressed in a c. jejuni fur mutant. an open reading frame encoding a fur homolog (designated perr for "peroxide stress regulator") was identified in the ...	1999	10515927
routine identification of campylobacter jejuni and campylobacter coli from human stool samples.	correct identification of campylobacter jejuni and campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. the widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are not generally available. this investigation was performed to assess the performance of common biochemical identification in comparison with species-specific pcr and gas liquid chro ...	1999	10518720
evaluation of a truncated recombinant flagellin subunit vaccine against campylobacter jejuni.	a recombinant protein comprising the maltose-binding protein (mbp) of escherichia coli fused to amino acids 5 to 337 of the flaa flagellin of campylobacter coli vc167 was evaluated for immunogenicity and protective efficacy against challenge by a heterologous strain of campylobacter, campylobacter jejuni 81-176, in two murine models. the sequence of the flaa gene of strain 81-176 revealed a predicted protein which was 98.1% similar to that of vc167 flaa over the region expressed in the fusion pr ...	1999	10531231
molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide o antigen of escherichia coli serotype o113.	shiga toxigenic escherichia coli (stec) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. within the stec family, eae-positive stec strains, particularly those belonging to serogroups o157 and o111, appear to have greater virulence for humans. however, in spite of being eae negative, stec strains belonging to serogroup o113 have frequently been associated with cases of severe stec disease, including hemolytic-uremic syndrome (hus). western blo ...	1999	10531250
recovery in embryonated eggs of viable but nonculturable campylobacter jejuni cells and maintenance of ability to adhere to hela cells after resuscitation.	the existence of a viable but nonculturable (vbnc) state has been described for campylobacter jejuni as it had been for a number pathogenic bacteria. three c. jejuni human isolates were suspended in surface water and subsequently entered the vbnc state. after starvation for 30 days, vbnc cells were inoculated in the yolk sacs of embryonated eggs. culturable cells were detected in a large proportion of the embryonated eggs inoculated with vbnc c. jejuni cells. recovered cells kept their adhesion ...	1999	10543837
large-scale survey of campylobacter species in human gastroenteritis by pcr and pcr-enzyme-linked immunosorbent assay.	a pcr-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening pcrs and species identification by pcr-enzyme-linked immunosorbent assay. this was applied to dna extracted from 3,738 fecal samples from patients with sporadic cases of acute gastroenteritis, submitted by seven regional public health laboratories in england and wales over a 2-year period. the sending laboratories had c ...	1999	10565897
rapid identification of campylobacter, arcobacter, and helicobacter isolates by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene.	a rapid two-step identification scheme based on pcr-restriction fragment length polymorphism (pcr-rflp) analysis of the 16s rrna gene was developed in order to differentiate isolates belonging to the campylobacter, arcobacter, and helicobacter genera. for 158 isolates (26 reference cultures and 132 clinical isolates), specific rflp patterns were obtained and species were successfully identified by this assay.	1999	10565952
helicobacter pylori and neutrophils: sialic acid-dependent binding to various isolated glycoconjugates.	helicobacter pylori has been shown to agglutinate erythrocytes in a sialic acid-dependent manner. however, very few studies have examined relevant target cells in the human stomach. neutrophils are required for the onset of gastritis, and the inflammatory reaction may be induced on contact between bacteria and neutrophils. in the present work, glycolipids and glycoproteins were isolated from neutrophils and were studied for binding by overlay with radiolabeled bacteria on thin-layer chromatogram ...	1999	10569742
a multifunctional atp-binding cassette transporter system from vibrio cholerae transports vibriobactin and enterobactin.	vibrio cholerae uses the catechol siderophore vibriobactin for iron transport under iron-limiting conditions. we have identified genes for vibriobactin transport and mapped them within the vibriobactin biosynthetic gene cluster. within this genetic region we have identified four genes, viup, viud, viug and viuc, whose protein products have homology to the periplasmic binding protein, the two integral cytoplasmic membrane proteins, and the atpase component, respectively, of other iron transport s ...	1999	10601218
[acute diarrhea in children less than 3 years of age: enteropathogens isolated in patient's stools, compared with a control group]	objectives: to observe the occurrence of different etiological agents of acute diarrhea (ad) in stool specimens of patients and children in a control group. material and methods: 100 children less than three years of age with ad were studied as well as 100 controls, between november 1993 and may 1994. stool specimens were collected in both groups and the following enteropathogens were searched for: rotavirus, escherichia coli (epec, etec, eiec, ehec), salmonella sp, shigella sp, campylobacter je ...	1999	14685548
prevalence of campylobacter spp isolated from the intestinal tract of pigs raised in an integrated swine production system.	to enumerate the prevalence of campylobacter isolates in the intestinal tract of market-weight swine raised in an integrated swine operation in texas.	1999	14567422
studies on enteric campylobacteriosis in tikur anbessa and ethio-swedish children's hospital, addis ababa, ethiopia.	between february 1992 and january 1993 different campylobacter spp. were isolated from 86 (13.7%) of 630 patients with diarrhoea at tikur anbessa and ethio-swedish children's hospital, addis ababa, ethiopia. in the same study population, shigella spp. were found in 11.7% and salmonella spp. in 3.8%. campylobacter spp. were found in all age groups, but the majority were isolated from children less than five years of age (68.6%). only 2 (0.9%) of 220 controls had campylobacter in their stools. of ...	1999	11957308
diagnostic potentials of the activities of lectins with whole cells of three bacterial diarrheagenic agents: aeromonas, plesiomonas, campylobacter.	the reactiviteisofstrains ofplesionmona shigeloides, campylobater jejuni, campylobacter coli and aeromonas species with some lectins namely soyabean agglutinin (sba), ricinus communis agglutinin (rca), peanut agllutinin (pna), cancanavalin-a (con-a), lima bean agglutinin (lma) and abrin were investigated using the microtitre plate method. the results obtained show that all stains of a. hydrophila, a. caviae, a. sobria and p. shigelloides reacted very strongly with pna and sba. all strains of c. ...	1998	17581001
differentiation of campylobacter isolates on the basis of sensitivity to boiling in water as measured by pcr-detectable dna.	differential sensitivity for the release of pcr-detectable genomic dna upon boiling in water is reported for 45 campylobacter jejuni and campylobacter coli strains isolated in egypt. all of the strains released pcr-detectable dna when treated with proteinase k and sodium dodecyl sulfate. when dna was extracted from these strains by boiling in water, nine (20%) of the strains were pcr negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.	1998	9435091
identification of campylobacter jejuni promoter sequences.	a promoterless lacz shuttle vector, which allowed screening of promoters by beta-galactosidase activity in campylobacter jejuni and escherichia coli, was developed. chromosomal dna fragments from c. jejuni were cloned into this vector; 125 of 1,824 clones displayed promoter activity in c. jejuni. eleven clones with strong promoter activity in c. jejuni were further characterized. their nucleotide sequences were determined, and the transcriptional start sites of the putative promoters in c. jejun ...	1998	9457862
effect of low temperatures on growth, structure, and metabolism of campylobacter coli sp10.	the effect of low temperatures on the survival, structure, and metabolism of campylobacter coli sp10, a virulent strain, was investigated. c. coli became nonculturable rapidly at 20 and 10 degree c and slightly later at 4 degrees c. incubation in a microaerobic atmosphere improved survival, but after day 8, campylobacters were detectable by direct-count procedures only. the increase in the number of coccoid cells was most pronounced at 37 degrees c but also was noticeable at 20 and 10 degrees c. ...	1998	9464397
extended survival and persistence of campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and -rrna staining.	in water microcosm experiments, the survival times of campylobacter isolates differed by up to twofold, as determined by culturing; this difference increased to fourfold when particular combinations of temperature and oxygenation were used. the mean survival times were much longer at 4 and 10 degrees c (202 and 176 h, respectively) than at 22 and 37 degrees c (43 and 22 h, respectively). the influence of anaerobiosis on survival time was less dramatic and differed considerably between isolates. ...	1998	9464415
serotyping scheme for campylobacter jejuni and campylobacter coli based on direct agglutination of heat-stable antigens.	campylobacter is now the most frequently reported cause of gastrointestinal disease in england and wales, yet few isolates are characterized beyond the genus level. the majority of isolates are campylobacter jejuni (90%), with most of the remainder being campylobacter coli. we describe an adaptation of the penner serotyping scheme in which passive hemagglutination has been replaced by detection of heat-stable antigens by direct bacterial agglutination; absorbed antisera are used where appropriat ...	1998	9466737
trna(arg) (fimu) and expression of sef14 and sef21 in salmonella enteritidis.	a tn10 insertion affecting sef14 fimbrial synthesis in salmonella enteritidis was located 13 bp upstream of a gene designated fimu. the 77-bp dna sequence of fimu from s. enteritidis was identical to that of fimu encoding trna(arg) (ucu) from salmonella typhimurium and 96% identical to that of the escherichia coli argu homolog. furthermore, the open reading frame adjacent to and overlapping the 3' end of fimu was similar to the prophage dlp12 integrase gene. the fimu-encoded transcript comigrate ...	1998	9473037
mutation in the peb1a locus of campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice.	campylobacter jejuni is one of the leading causes of bacterial diarrhea throughout the world. we previously found that peb1 is a homolog of cluster 3 binding proteins of bacterial abc transporters and that a c. jejuni adhesin, cell-binding factor 1 (cbf1), if not identical to, contains peb1. a single protein migrating at approximately 27 to 28 kda was recognized by anti-cbf1 and anti-peb1. to determine the role that the operon encoding peb1 plays in c. jejuni adherence, peb1a, the gene encoding ...	1998	9488379
the pseudomonas aeruginosa flagellar cap protein, flid, is responsible for mucin adhesion.	mucin-specific adhesion of pseudomonas aeruginosa plays an important role in the initial colonization of this organism in the airways of cystic fibrosis patients. we report here that the flagellar cap protein, flid, participates in this adhesion process. a polar chromosomal insertional mutation in the p. aeruginosa flid gene made this organism nonadhesive to mucin in an in vitro mucin adhesion assay. the adhesive phenotype was restored by providing the flid gene alone on a multicopy plasmid, sug ...	1998	9488388
variation in flagellin genes and proteins of burkholderia cepacia.	the majority of isolates of burkholderia cepacia, an important opportunistic pathogen associated with cystic fibrosis, can be classified into two types on the basis of flagellin protein size. electron microscopic analysis indicates that the flagella of strains with the larger flagellin type (type i) are wider in diameter. flagellin genes representative of both types were cloned and sequenced to design oligonucleotide primers for pcr amplification of the central variable domain of b. cepacia flag ...	1998	9495748
helicobacter pylori porcdab and oordabc genes encode distinct pyruvate:flavodoxin and 2-oxoglutarate:acceptor oxidoreductases which mediate electron transport to nadp.	helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. previous studies (n. j. hughes, p. a. chalk, c. l. clayton, and d. j. kelly, j. bacteriol. 177:3953-3959, 1995) have indicated that the major routes for the generation of acetyl coenzyme a (acetyl-coa) and succinyl-coa are via pyruvate:flavodoxin oxidoreductase (por) and 2-oxoglutarate:acceptor oxidoreductase (oor), respectively. th ...	1998	9495749
transcription of ppk from acinetobacter sp. strain adp1, encoding a putative polyphosphate kinase, is induced by phosphate starvation.	polyphosphate kinase (ppk) catalyzes the formation of polyphosphate from atp. we cloned the ppk gene (2,073 bp) from acinetobacter sp. strain adp1; this gene encodes a putative polypeptide of 78.6 kda with extensive homology to polyphosphate kinase from escherichia coli and other bacteria. chromosomal disruption of ppk by inserting a transcriptionally fused lacz does not affect growth under conditions of phosphate limitation or excess. beta-galactosidase activity expressed from the single-copy p ...	1998	9501429
ribosomal rna gene restriction fragment diversity amongst penner serotypes of campylobacter jejuni and campylobacter coli.	diversity based on ribosomal rna gene-restriction endonuclease digest patterns was detected amongst forty-seven strains of campylobacter made up of 38 strains of campylobacter jejuni and 9 strains of campylobacter coli. restriction digests of chromosomal dna prepared by treating with hae iii were probed with an oligonucleotide specific for campylobacter 16s ribosomal rna genes. seventeen distinct hybridization patterns, each indicating the presence of 2-4 copies of the 16s rrna gene are encoded ...	1998	9528123
a cyanobacterium lacking iron superoxide dismutase is sensitized to oxidative stress induced with methyl viologen but is not sensitized to oxidative stress induced with norflurazon	a strain of synechococcus sp. strain pcc 7942 with no functional fe superoxide dismutase (sod), designated sodb-, was characterized by its growth rate, photosynthetic pigments, and cyclic photosynthetic electron transport activity when treated with methyl viologen or norflurazon (nf). in their unstressed conditions, both the sodb- and wild-type strains had similar chlorophyll and carotenoid contents and catalase activity, but the wild type had a faster growth rate and higher cyclic electron tran ...	1998	9536078
a rapid pcr-based dna test for enterotoxic bacillus cereus.	the occurrence of dna sequences encoding the hemolysin hbla complex and bacillus cereus enterotoxin bcet, which have recently been confirmed as enterotoxins, was studied in bacillus spp. to amplify these dna sequences, pcr primer systems for the b component of hbla and for bcet dna sequences were developed. the results from the amplification of hbla sequences correlated well with results obtained with the b. cereus enterotoxin (diarrheal type) test kit (rpla kit), but not with the results of the ...	1998	9572928
evidence of genomic instability in campylobacter jejuni isolated from poultry.	poultry isolates of campylobacter jejuni derived from a survey of meat processing batches were genotyped by pulsed-field gel electrophoresis (pfge) of chromosomal dna to establish the clonal relationships between single-colony isolates. in the majority of batches studied, one or two genotype patterns predominated. however, in one batch (batch a), 21 single-colony isolates gave 14 different pfge genotypes. the banding patterns obtained with smai were sufficiently different to distinguish between ...	1998	9572956
helicobacter pylori glutamine synthetase lacks features associated with transcriptional and posttranslational regulation.	helicobacter pylori urease, produced in abundance, is indispensable for the survival of h. pylori in animal hosts. urea is hydrolyzed by the enzyme, resulting in the liberation of excess ammonia, some of which neutralizes gastric acid. the remaining ammonia is assimilated into protein by glutamine synthetase (ec 6.3.1.2), which catalyzes the reaction: nh3 + glutamate + atp-->glutamine + adp + pi. we hypothesized that glutamine synthetase plays an unusually critical role in nitrogen assimilation ...	1998	9573059
campylobacter jejuni cytolethal distending toxin causes a g2-phase cell cycle block.	cytolethal distending toxin (cdt) from the diarrheagenic bacterium campylobacter jejuni was shown to cause a rapid and specific cell cycle arrest in hela and caco-2 cells. within 24 h of treatment, cdt caused hela cells to arrest with a 4n dna content, indicative of cells in g2 or early m phase. immunofluorescence studies indicated that the arrested cells had not entered m phase, since no evidence of tubulin reorganization or chromatin condensation was visible. cdt treatment was also shown to ca ...	1998	9573072
characterization of chemotactic responses and flagella of hyphomicrobium strain w1-1b.	motile swarmer cells of hyphomicrobium strain w1-1b displayed positive chemotactic responses toward methylamine, dimethylamine, and trimethylamine but did not display significant chemotactic responses towards methanol and arginine. electron micrographs of negatively stained intact flagellar filaments indicated a novel striated surface pattern. the flagella were composed of two proteins of 39 and 41 kda. neither protein was a glycoprotein as determined by schiff's staining and by enzyme immunoass ...	1998	9603896
typing of human campylobacter jejuni isolates in finland by pulsed-field gel electrophoresis.	a total of 69 pulsed-field gel electrophoresis (pfge) types were identified among 176 campylobacter jejuni isolates from finnish patients. in two geographic areas studied, five predominant pfge types comprised over 40% of the isolates. one-third of the isolates had unique pfge types. in small outbreaks, identical pfge patterns were demonstrated, indicating a common source of infection.	1998	9620423
cloning and comparison of flic genes and identification of glycosylation in the flagellin of pseudomonas aeruginosa a-type strains.	pseudomonas aeruginosa a-type strains produce flagellin proteins which vary in molecular weight between strains. to compare the properties of a-type flagellins, the flagellin genes of several pseudomonas aeruginosa a-type strains, as determined by interaction with specific anti-a monoclonal antibody, were cloned and sequenced. pcr amplification of the a-type flagellin gene fragments from five strains each yielded a 1.02-kb product, indicating that the gene size is not likely to be responsible fo ...	1998	9620973