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fecal shedding of campylobacter jejuni and campylobacter coli among feral pigs in texas.the population and range of feral pigs in the united states are rapidly expanding, yet key knowledge gaps exist regarding their role in the ecology and transmission of foodborne pathogens. our objectives were to estimate the prevalence of campylobacter jejuni and campylobacter coli shedding among feral pigs throughout texas and to identify risk factors for positive status. faecal samples were collected from feral pigs in texas from february 2014 through may 2015, and target organisms were detect ...201728834190
survival of selected foodborne pathogens on dry cured pork loins.the safety of ready-to-eat products such as cured pork loins must be guaranteed by the food industry. in the present study, the efficacy of the dry curing process of pork loins obtained from free-range pigs in the reduction of three of the most important foodborne pathogens is analysed. a total of 28 pork loin segments, with an average weight of 0.57±0.12kg, were divided into four groups with three being inoculated by immersion with 7logcfu/ml of either salmonella typhimurium, campylobacter coli ...201728759797
cardamom, cumin, and dill weed essential oils: chemical compositions, antimicrobial activities, and mechanisms of action against campylobacter spp.natural antimicrobials as well as essential oils (eos) have gained interest to inhibit pathogenic microorganisms and to control food borne diseases. campylobacter spp. are one of the most common causative agents of gastroenteritis. in this study, cardamom, cumin, and dill weed eos were evaluated for their antibacterial activities against campylobacter jejuni and campylobacter coli by using agar-well diffusion and broth microdilution methods, along with the mechanisms of antimicrobial action. che ...201728714890
genetic diversity of thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina.the objective of this study was to investigate a clonal relationship among thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. a total of 128 thermotolerant campylobacter spp. (89 c. jejuni and 39 c. coli) isolates from six poultry meat chains were examined. these isolates were from: a) hens from breeder flocks, b) chickens on the farm (at ages 1 wk and 5 wk), c) chicken carcasses in the slaughterhouse, and d) chicken carcasses in the r ...201728712509
no clear differences between organic or conventional pig farms in the genetic diversity or virulence of campylobacter coli isolates.to evaluate the impact of pig farm management on the genetic diversity and on the virulence of campylobacter coli, we characterized isolates from 19 organic pig farms (62 isolates) and from 24 conventional pig farms (58 isolates). the 120 c. coli isolates were typed using pulsed field gel electrophoresis (pfge) and multilocus sequence typing (mlst) and the presence of nine virulence genes was screened using real-time pcr. the capacity of adhesion and invasion of 61 isolates (32 from organic and ...201728694791
a study on campylobacter jejuni and campylobacter coli through commercial broiler production chains in thailand: antimicrobial resistance, the characterization of dna gyrase subunit a mutation, and genetic diversity by flagellin a gene restriction fragment length polymorphism.contaminated poultry meat is regarded as the main source of human campylobacteriosis. during september 2014 and february 2015, breeder flocks, hatcheries, and broiler farms from two chicken production chains were investigated chronologically. five commercial breeder flocks (breeder flocks 1-5), two hatcheries (hatcheries a and b), and five broiler flocks (broiler flocks 1-5) were sampled in this study. campylobacter colonization of both breeder and broiler flocks was determined from cloacal swab ...201728665716
campylobacter species isolated from pigs in grenada exhibited novel clones: genotypes and antimicrobial resistance profiles of sequence types.infections caused by campylobacter species pose a severe threat to public health worldwide. however, in grenada, the occurrence and characteristics of campylobacter in food animals, including pigs, remain mostly unknown. in this study, we identified the sequence types (sts) of campylobacter from young healthy pigs in grenada and compared the results with previous studies in grenada and other countries. antimicrobial resistance patterns and diversity of the campylobacter clones were evaluated. ni ...201728650672
impact of built-up-litter and commercial antimicrobials on salmonella and campylobacter contamination of broiler carcasses processed at a pilot mobile poultry-processing unit.the small-scale mobile poultry-processing unit (mppu) produced raw poultry products are of particular food safety concern due to exemption of usda poultry products inspection act. limited studies reported the microbial quality and safety of mppu-processed poultry carcasses. this study evaluated the salmonella and campylobacter prevalence in broiler ceca and on mppu-processed carcasses and efficacy of commercial antimicrobials against campylobacter jejuni on broilers. in study i, straight-run hub ...201728649571
campylobacter coli in organic and conventional pig production in france and sweden: prevalence and antimicrobial resistance.the purpose of the study was to evaluate and compare the prevalence and antimicrobial resistance of campylobacter coli in conventional and organic pigs from france and sweden. fecal or colon samples were collected at farms or at slaughterhouses and cultured for campylobacter. the minimum inhibitory concentrations of ciprofloxacin, nalidixic acid, streptomycin, tetracycline, erythromycin, and gentamicin were determined by microdilution for a total of 263 french strains from 114 pigs from 50 diffe ...201728611754
irrigation water sources and time intervals as variables on the presence of campylobacter spp. and listeria monocytogenes on romaine lettuce grown in muck soil.irrigation water has been identified as a possible source of vegetable contamination by foodborne pathogens. risk management for pathogens such as campylobacter spp. and listeria monocytogenes in fields can be influenced by the source of the irrigation water and the time interval between last irrigation and harvest. plots of romaine lettuce were irrigated with manure-contaminated water or aerated pond water 21, 7, or 3 days prior to harvesting, and water and muck soil samples were collected at e ...201728604176
detection and prevalence of antimicrobial resistance genes in <i>campylobacter</i> spp. isolated from chickens and humans.campylobacter spp. are common pathogenic bacteria in both veterinary and human medicine. infections caused by campylobacter spp. are usually treated using antibiotics. however, the injudicious use of antibiotics has been proven to spearhead the emergence of antibiotic resistance. the purpose of this study was to detect the prevalence of antibiotic resistance genes in campylobacter spp. isolated from chickens and human clinical cases in south africa. one hundred and sixty one isolates of campylob ...201728582978
dissemination of erm(b) and its associated multidrug-resistance genomic islands in campylobacter from 2013 to 2015.a total of 1372 campylobacter isolates (1107 campylobacter coli and 265 campylobacter jejuni) were obtained from 3462 samples collected from slaughterhouses and farms in three representative regions of china (shandong, guangdong, and shanghai) over three successive years (2013-2015). of these, 84 (84/1372, 6.1%) were erm(b)-positive, and all 84 positive isolates were identified as c. coli (83 chicken isolates and one swine isolate). the prevalence of erm(b) in campylobacter isolates was compared ...201728532801
prevalence of campylobacter species in wild birds of south korea.campylobacter species cause human gastrointestinal infections worldwide. they commonly inhabit intestines of avian species including wild birds. they might play a role in the spread of infections to humans and other bird species. the prevalence of campylobacter species in 2164 faecal samples of wild birds (representing 71 species and 28 families) captured across the korean peninsula was evaluated in this study. the overall prevalence was 15.3% (332/2164). bird species belonging to the family cha ...201728503965
novel sampling method for assessing human-pathogen interactions in the natural environment using boot socks and citizen scientists, with application to campylobacter seasonality.this paper introduces a novel method for sampling pathogens in natural environments. it uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). this sampling method is implemented using a citizen science approach: groups of three walkers wearing boot socks undertook one of six routes, 40 times over 16 months in the north west ...201728500040
identification and differentiation of campylobacter species by high-resolution melting curve analysis.campylobacter jejuni and campylobacter coli are the important food-born zoonotic pathogen, also are leading causes of human food borne illnesses worldwide. cadf gene is expressed in all c. jejuni and c. coli strains and mediates cell binding to the cell matrix protein, fibronectin. high-resolution melting (hrm) analysis is emerging as an efficient and robust method for discriminating dna sequence variants. the goal of this study was to apply hrm analysis to identification of c. jejuni and c. col ...201728478199
simultaneous identification of campylobacter jejuni, campylobacter coli, and campylobacter lari with smartcycler-based multiplex quantitative polymerase chain reaction.consumption of campylobacter contaminated food or water is a leading cause of human acute gastroenteritis. campylobacter jejuni, campylobacter coli, and campylobacter lari account for over 95% of total campylobacter infections. a multiplex quantitative polymerase chain reaction (qpcr) for simultaneous identification of c. jejuni, c. coli, and c. lari was developed for use with the smartcycler ii system.201728440688
lauric acid as feed additive - an approach to reducing campylobacter spp. in broiler meat.the increasing prevalence of campylobacter spp. within broiler populations is a major problem for food safety and consumer protection worldwide. in vitro studies could already demonstrate that campylobacter spp. are susceptible to lauric acid. the purpose of this study was to examine in vivo the influence of lauric acid as a feed additive on slaughter parameters, muscle fatty acid profile, meat quality traits and the reduction of campylobacter coli in inoculated meat of ross 308 (r308) and hubba ...201728419122
postchill antimicrobial treatments to control salmonella, listeria, and campylobacter contamination on chicken skin used in ground chicken.ground poultry products are frequently contaminated with foodborne pathogens. with the potential for increased regulatory scrutiny, it is important to use sufficient intervention strategies to control pathogen levels effectively. a large proportion of the bacteria introduced to ground chicken are likely to come from broiler skin, which is added to achieve target fat content and maintain product texture and taste. in this research, antimicrobials, including 50 ppm of chlorine and 1,200 ppm of per ...201728414258
the current state of macrolide resistance in campylobacter spp.: trends and impacts of resistance mechanisms.campylobacter spp., especially campylobacter jejuni and c. coli, are leading bacterial foodborne pathogens worldwide. in the united states, an estimated 0.8 million cases of campylobacteriosis occur annually, mostly involving c. jejuni campylobacteriosis is generally self-limiting, but in severe cases, treatment with antibiotics may be mandated. the increasing incidence of fluoroquinolone resistance in campylobacter has rendered macrolides such as erythromycin and azithromycin the drugs of choic ...201728411226
emergence of a plasmid-borne multidrug resistance gene cfr(c) in foodborne pathogen campylobacter.to identify and characterize a novel cfr variant that recently emerged and confers multidrug resistance in campylobacter , a major foodborne pathogen.201728186558
restriction glycosylases: involvement of endonuclease activities in the restriction process.all restriction enzymes examined are phosphodiesterases generating 3΄-oh and 5΄-p ends, but one restriction enzyme (restriction glycosylase) excises unmethylated bases from its recognition sequence. whether its restriction activity involves endonucleolytic cleavage remains unclear. one report on this enzyme, r.pabi from a hyperthermophile, ascribed the breakage to high temperature while another showed its weak ap lyase activity generates atypical ends. here, we addressed this issue in mesophiles ...201728180312
characterisation of aerotolerant forms of a robust chicken colonizing campylobacter coli.campylobacter contaminated poultry meat is a major source of human foodborne illness. campylobacter coli strain or12 is a robust colonizer of chickens that was previously shown to outcompete and displace other campylobacter strains from the chicken's gastrointestinal tract. this strain is capable of aerobic growth on blood agar. serial aerobic passage increased this aerotolerance as assessed by quantitative assays for growth and survival on solid media. aerotolerance was also associated with inc ...201728396658
high prevalence and predominance of the aph(2″)-if gene conferring aminoglycoside resistance in campylobacter.campylobacter is a major foodborne pathogen and previous studies revealed that campylobacter isolates from food-producing animals are increasingly resistant to gentamicin in china. the molecular epidemiology and genetic mechanisms responsible for gentamicin resistance in china have not been well understood. in this study, 607 campylobacter isolates of chicken and swine origins collected in the year of 2014 were analyzed, revealing that 15.6% (25/160) of the c. jejuni isolates and 79.9% (357/447) ...201728264854
antimicrobial resistance of campylobacter species isolated from broilers in live bird markets in shanghai, china.this study was conducted to determine the prevalence of antimicrobial resistance in campylobacter spp. isolates from broilers in live bird markets (lbms). a total of 209 campylobacter spp. isolates (84 campylobacter jejuni; 125 campylobacter coli) were recovered from 364 broiler cecum samples collected from five lbms in shanghai, china. minimum inhibitory concentrations of 13 antimicrobials were determined using agar dilution method. more than 96% of the campylobacter spp. isolates were resistan ...201727854542
whole genome sequencing and analysis of campylobacter coli yh502 from retail chicken reveals a plasmid-borne type vi secretion system.campylobacter is a major cause of foodborne illnesses worldwide. campylobacter infections, commonly caused by ingestion of undercooked poultry and meat products, can lead to gastroenteritis and chronic reactive arthritis in humans. whole genome sequencing (wgs) is a powerful technology that provides comprehensive genetic information about bacteria and is increasingly being applied to study foodborne pathogens: e.g., evolution, epidemiology/outbreak investigation, and detection. herein we report ...201728217442
temporal induction of pro-inflammatory and regulatory cytokines in human peripheral blood mononuclear cells by campylobacter jejuni and campylobacter coli.campylobacter jejuni along with c. coli are major cause of human gastroenteritis worldwide. so far, the human immune response against campylobacter is not entirely clear. we hypothesize that it is coordinated by an interaction between pro-inflammatory and regulatory cytokines which is influenced by bacterial and host-individual differences. accordingly, we used peripheral blood mononuclear cells (pbmc) from healthy donors to study the primary systemic immune response to c. jejuni and c. coli. pb ...201728196097
genetic diversity and antimicrobial resistance profiles of campylobacter coli and campylobacter jejuni isolated from broiler chicken in farms and at time of slaughter in central italy.genetic diversity and antimicrobial resistance of campylobacter coli and campylobacter jejuni were investigated along the broiler chicken production chain in central italy.201728194914
isolation, identification and differentiation of campylobacter spp. using multiplex pcr assay from goats in khartoum state, sudan.the aim of this study was to identify and characterize thermophilic campylobacter species in faecal samples from goats in khartoum state, sudan, by application of multiplex polymerase chain reaction. campylobacteriosis is a zoonotic disease of global concern, and the organisms can be transmitted to human via food, water and through contact with farm animals and pets. there are five clinically related campylobacter species: campylobacter jejuni (c. jejuni). campylobacter coli, campylobacter lari, ...201728160162
reply to "activity of fosfomycin on clinical isolates of campylobacter jejuni and campylobacter coli of enteric origin". 201728119318
activity of fosfomycin on clinical isolates of campylobacter jejuni and campylobacter coli of enteric origin. 201728119317
prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from children and environmental sources in urban and suburban areas.campylobacteriosis is a dominant bacterial cause of foodborne infection and is considered the main public health problem in europe and many other countries worldwide. in the study lasting from 2011 to 2013 we compared the prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from children, domestic animals, poultry meat and surface water in northern poland.201728376713
faecal campylobacter shedding among dogs in animal shelters across texas.epidemiologic studies on faecal campylobacter shedding among dogs in the united states have been limited, despite evidence that the incidence of human campylobacteriosis has increased over the last decade. our objectives were to estimate the prevalence of faecal campylobacter shedding among shelter dogs in texas, to estimate the specific prevalence of campylobacter jejuni and campylobacter coli shedding, and to identify risk factors for campylobacter-positive status. using a cross-sectional stud ...201728371319
prevalence, phenotypic and genetic diversity of campylobacter in poultry fresh meat and poultry products on retail sale in tuscany (italy).in this study, the prevalence of campylobacter spp. in poultry fresh meat and ready‑to‑cook products was evaluated. seventy‑three samples were collected at retail level from supermarkets and discount stores, obtaining 61.6% positivity. of 133 campylobacter isolates, 86 strains (campylobacter coli, 58.1% and campylobacter jejuni, 41.9%) were selected for characterisation on the basis of their smai and kpni pulsed field gel‑electrophoresis (pfge) profiles, to exclude clonal replicates. campylobact ...201728365923
further characterization and independent validation of a dna aptamer-quantum dot-based magnetic sandwich assay for campylobacter.previously reported dna aptamers developed against surface proteins extracted from campylobacter jejuni were further characterized by aptamer-based western blotting and shown to bind epitopes on proteins weighing ~16 and 60 kd from reduced c. jejuni and campylobacter coli lysates. proteins of these approximate weights have also been identified in traditional antibody-based western blots of campylobacter spp. specificity of the capture and reporter aptamers from the previous report was further va ...201728342148
prevalence, antimicrobial resistance and genetic diversity of campylobacter coli and campylobacter jejuni in ecuadorian broilers at slaughter age.thermotolerant campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. the linkage of human campylobacteriosis and poultry has been widely described. in this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of c. coli and c. jejuni in broilers from ecuador. caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of pichincha durin ...201728339716
a quantitative real-time pcr approach for assessing campylobacter jejuni and campylobacter coli colonization in broiler herds.human campylobacteriosis is a major public health concern in developed countries, with campylobacter jejuni and campylobacter coli from poultry recognized as the main source of human infection. identification of campylobacter-positive broiler herds before slaughter is essential for implementing measures to avoid carryover of pathogens via the slaughter process into the food chain. however, appropriate methods that have been validated for testing poultry flocks antemortem are lacking for campylob ...201728282226
occurrence and genotypes of campylobacter species in broilers during the rearing period.poultry are the main source of campylobacter infection worldwide. to obtain information on campylobacter-infected flocks and create a reference for preventing and controlling campylobacter at farm level, campylobacter isolates were recovered from broilers and the environments of nine chicken flocks in two farms during growth. the genetic relationship between the campylobacter isolates was determined using multilocus sequence typing. flocks were colonized as early as 3 weeks after introduction to ...201727766896
multiplex pcr assay for identifi cation and differentiation of campylobacter jejuni and campylobacter coli isolates.campylobacter spp. are important causative agents of gastrointestinal infections in humans. the most frequently isolated strains of this bacterial genus are campylobacter jejuni and campylobacter coli. to date, genetic methods for bacterial identification have not been used in bulgaria. we optimized the multiplex psr assay to identify campylobacter spp. and differentiate c. jejuni from c. coli in clinical isolates. we also compared this method with the routinely used biochemical methods.201727552785
macrolide resistance mechanisms and virulence factors in erythromycin-resistant campylobacter species isolated from chicken and swine feces and carcasses.resistance to antimicrobials was measured in 73 isolates of campylobacter jejuni (c. jejuni) and 121 isolates of campylobacter coli (c. coli) from chicken and swine feces and carcasses in korea. both bacterial species showed the highest resistance to (fluoro) quinolones (ciprofloxacin and nalidixic acid) out of the nine antimicrobials tested. erythromycin resistance was much higher in c. coli (19.0%, 23/121) than in c. jejuni (6.8%, 5/73). the mutation in the 23s rrna gene was primarily responsi ...201727593510
campylobacter coli infection in pet birds in southern italy.avian species are considered as the main reservoir of campylobacter spp. however, few data are available on the presence of this microorganism in pet birds. this study was therefore performed to determine the prevalence of campylobacter spp. in pet birds bred in southern italy. faecal samples were collected from 88 cages housing different species of pet birds and examined by bacteriological culture and polymerase chain reaction. a total of 13.6% of the cage samples were positive for campylobacte ...201728061877
first case report of myopericarditis linked to campylobacter coli enterocolitis.campylobacter spp. are a common cause of mostly self-limiting enterocolitis. although rare, pericarditis and myopericarditis have been increasingly documented as complications following campylobacteriosis. such cases have occurred predominantly in younger males, and involved a single causative species, namely campylobacter jejuni. we report the first case of myopericarditis following campylobacter coli enterocolitis, with illness occurring in an immunocompetent middle-aged female.201728056838
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