Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| pcr detection, identification to species level, and fingerprinting of campylobacter jejuni and campylobacter coli direct from diarrheic samples. | three sets of primers were designed for pcr detection and differentiation of campylobacter jejuni and campylobacter coli. the first pcr assay was designed to coidentify c. jejuni and c. coli based on their 16s rrna gene sequences. the second pcr assay, based on the hippuricase gene sequence, identified all tested reference strains of c. jejuni and also strains of that species which lack detectable hippuricase activity. the third pcr assay, based on the sequence of a cloned (putative) aspartokina ... | 1997 | 9316909 |
| expression of campylobacter hyoilei lipo-oligosaccharide (los) antigens in escherichia coli. | campylobacter spp. are well recognized as primary pathogens in animals and in people. to isolate and define the genetic regions encoding major surface antigens of campylobacter hyoilei, genomic dna of the type strain of the species, rmit-32a, was cloned into a cosmid vector, pla2917, in escherichia coli and the resulting genomic library was screened using antiserum raised to the parent c. hyoilei strain. six cosmid clones were found to express a series of immunoreactive bands in the 15-25 kda ra ... | 1997 | 9387226 |
| analysis of distribution of campylobacter jejuni and campylobacter coli in broilers by using restriction fragment length polymorphism of flagellin gene. | the incidence of campylobacter jejuni and campylobacter coli in broiler farms was 33.9% (19/56). c. jejuni-positive flocks accounted for 20.0% (17/85) and c. coli-positive ones was 4.7% (4/85). there were 14 patterns (fla type) of restriction fragment length polymorphism (rflp) of flagellin a gene among these 22 strains of c. jejuni and c. coli including the standard strain c. jejuni atcc 33560. different fla types of campylobacter were isolated from broilers in different growing cycles on the s ... | 1997 | 9409516 |
| the serotype distribution of campylobacter jejuni and c. coli isolated from patients with diarrhoea and controls at tikur anbassa hospital, addis ababa, ethiopia. | sixty-eight isolates of campylobacter jejuni and c. coli isolated from patients with diarrhoea (n = 630) and controls (n = 220) at tikur anbassa hospital, addis ababa, ethiopia were serotyped on the basis of the heat-labile (hl) and the heat-stable (hs) antigens, by using 16 and 34 antisera, respectively, for the two methods. with the antisera against heat labile antigens, 89.3% of the c. jejuni and 75% of the c. coli were typable. the hl serotypes 1, 2, 3, 4, 5, 6 and 7 were the most common amo ... | 1997 | 9129584 |
| the flge gene of campylobacter coli is under the control of the alternative sigma factor sigma54. | the flge gene encoding the flagellar hook protein of campylobacter coli vc167-t1 was cloned by immunoscreening of a genomic library constructed in lambdazap express. the flge dna sequence was 2,553 bp in length and encoded a protein with a deduced molecular mass of 90,639 da. the sequence had significant homology to the 5' and 3' sequences of the flge genes of helicobacter pylori, treponema phagedenis, and salmonella typhimurium. primer extension analysis indicated that the vc167 flge gene is co ... | 1997 | 9244248 |
| differentiation of campylobacter isolates on the basis of sensitivity to boiling in water as measured by pcr-detectable dna. | differential sensitivity for the release of pcr-detectable genomic dna upon boiling in water is reported for 45 campylobacter jejuni and campylobacter coli strains isolated in egypt. all of the strains released pcr-detectable dna when treated with proteinase k and sodium dodecyl sulfate. when dna was extracted from these strains by boiling in water, nine (20%) of the strains were pcr negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes. | 1998 | 9435091 |
| ribosomal rna gene restriction fragment diversity amongst penner serotypes of campylobacter jejuni and campylobacter coli. | diversity based on ribosomal rna gene-restriction endonuclease digest patterns was detected amongst forty-seven strains of campylobacter made up of 38 strains of campylobacter jejuni and 9 strains of campylobacter coli. restriction digests of chromosomal dna prepared by treating with hae iii were probed with an oligonucleotide specific for campylobacter 16s ribosomal rna genes. seventeen distinct hybridization patterns, each indicating the presence of 2-4 copies of the 16s rrna gene are encoded ... | 1998 | 9528123 |
| a cyanobacterium lacking iron superoxide dismutase is sensitized to oxidative stress induced with methyl viologen but is not sensitized to oxidative stress induced with norflurazon | a strain of synechococcus sp. strain pcc 7942 with no functional fe superoxide dismutase (sod), designated sodb-, was characterized by its growth rate, photosynthetic pigments, and cyclic photosynthetic electron transport activity when treated with methyl viologen or norflurazon (nf). in their unstressed conditions, both the sodb- and wild-type strains had similar chlorophyll and carotenoid contents and catalase activity, but the wild type had a faster growth rate and higher cyclic electron tran ... | 1998 | 9536078 |
| a rapid pcr-based dna test for enterotoxic bacillus cereus. | the occurrence of dna sequences encoding the hemolysin hbla complex and bacillus cereus enterotoxin bcet, which have recently been confirmed as enterotoxins, was studied in bacillus spp. to amplify these dna sequences, pcr primer systems for the b component of hbla and for bcet dna sequences were developed. the results from the amplification of hbla sequences correlated well with results obtained with the b. cereus enterotoxin (diarrheal type) test kit (rpla kit), but not with the results of the ... | 1998 | 9572928 |
| evidence of genomic instability in campylobacter jejuni isolated from poultry. | poultry isolates of campylobacter jejuni derived from a survey of meat processing batches were genotyped by pulsed-field gel electrophoresis (pfge) of chromosomal dna to establish the clonal relationships between single-colony isolates. in the majority of batches studied, one or two genotype patterns predominated. however, in one batch (batch a), 21 single-colony isolates gave 14 different pfge genotypes. the banding patterns obtained with smai were sufficiently different to distinguish between ... | 1998 | 9572956 |
| helicobacter pylori glutamine synthetase lacks features associated with transcriptional and posttranslational regulation. | helicobacter pylori urease, produced in abundance, is indispensable for the survival of h. pylori in animal hosts. urea is hydrolyzed by the enzyme, resulting in the liberation of excess ammonia, some of which neutralizes gastric acid. the remaining ammonia is assimilated into protein by glutamine synthetase (ec 6.3.1.2), which catalyzes the reaction: nh3 + glutamate + atp-->glutamine + adp + pi. we hypothesized that glutamine synthetase plays an unusually critical role in nitrogen assimilation ... | 1998 | 9573059 |
| campylobacter jejuni cytolethal distending toxin causes a g2-phase cell cycle block. | cytolethal distending toxin (cdt) from the diarrheagenic bacterium campylobacter jejuni was shown to cause a rapid and specific cell cycle arrest in hela and caco-2 cells. within 24 h of treatment, cdt caused hela cells to arrest with a 4n dna content, indicative of cells in g2 or early m phase. immunofluorescence studies indicated that the arrested cells had not entered m phase, since no evidence of tubulin reorganization or chromatin condensation was visible. cdt treatment was also shown to ca ... | 1998 | 9573072 |
| characterization of chemotactic responses and flagella of hyphomicrobium strain w1-1b. | motile swarmer cells of hyphomicrobium strain w1-1b displayed positive chemotactic responses toward methylamine, dimethylamine, and trimethylamine but did not display significant chemotactic responses towards methanol and arginine. electron micrographs of negatively stained intact flagellar filaments indicated a novel striated surface pattern. the flagella were composed of two proteins of 39 and 41 kda. neither protein was a glycoprotein as determined by schiff's staining and by enzyme immunoass ... | 1998 | 9603896 |
| typing of human campylobacter jejuni isolates in finland by pulsed-field gel electrophoresis. | a total of 69 pulsed-field gel electrophoresis (pfge) types were identified among 176 campylobacter jejuni isolates from finnish patients. in two geographic areas studied, five predominant pfge types comprised over 40% of the isolates. one-third of the isolates had unique pfge types. in small outbreaks, identical pfge patterns were demonstrated, indicating a common source of infection. | 1998 | 9620423 |
| cloning and comparison of flic genes and identification of glycosylation in the flagellin of pseudomonas aeruginosa a-type strains. | pseudomonas aeruginosa a-type strains produce flagellin proteins which vary in molecular weight between strains. to compare the properties of a-type flagellins, the flagellin genes of several pseudomonas aeruginosa a-type strains, as determined by interaction with specific anti-a monoclonal antibody, were cloned and sequenced. pcr amplification of the a-type flagellin gene fragments from five strains each yielded a 1.02-kb product, indicating that the gene size is not likely to be responsible fo ... | 1998 | 9620973 |
| an outbreak of infectious hepatitis in commercially reared ostriches associated with campylobacter coli and campylobacter jejuni. | a disease causing high morbidity and mortality was observed in young ostriches from six properties in southeast queensland, australia. the disease affected birds from 2-8 weeks of age and was characterised clinically by bright-green urates and pathologically by severe necrotic hepatitis. the liver lesions resembled those of vibrionic hepatitis in other avian species. campylobacter coli was isolated from the livers of affected ostriches from five of the six properties. campylobacter jejuni subsp. ... | 1998 | 9631530 |
| heterogeneity in levels of vacuolating cytotoxin gene (vaca) transcription among helicobacter pylori strains. | broth culture supernatants from tox+ helicobacter pylori strains induce vacuolation of hela cells in vitro and contain vaca in concentrations that are higher than those found in supernatants from tox- h. pylori strains. to investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cytotoxin gene (vaca) in eight tox+ strains (each with a type s1/m1 vaca genotype) and nine tox- strains (each with a type s2/m2 vaca genotype). most of the tox+ and tox- strains tested ... | 1998 | 9632570 |
| isolation and molecular analysis of colonising and non-colonising strains of campylobacter jejuni and campylobacter coli following experimental infection of young chickens. | fourteen-day-old chickens were inoculated with selected campylobacter coli and c. jejuni strains. c. jejuni strains were of two subgroups based on a polymorphism detected using a dna probe and represented the profiles typical for the majority of strains of either chicken or human origin. all c. coli strains previously isolated from humans colonised chickens, whereas from 4/7 c. jejuni strains of human origin, failed to colonise. of 12 campylobacter strains of chicken origin, 10 established a per ... | 1998 | 9646454 |
| epidemiological typing of campylobacter isolates from meat processing plants by pulsed-field gel electrophoresis, fatty acid profile typing, serotyping, and biotyping. | campylobacter spp. are a leading cause of bacterial gastroenteritis. foods of animal origin, particularly under-cooked poultry, are common sources of campylobacter species associated with disease in humans. a collection of 110 campylobacter jejuni and 31 c. coli human and environmental isolates from different ontario, canada, abattoirs were analyzed by pulsed-field gel electrophoresis, fatty acid profile typing, and biotyping. previously collected serotyping data for the same isolates were also ... | 1998 | 9647797 |
| microtiter assay for detecting campylobacter spp. and helicobacter pylori with surface gangliosides which bind cholera toxin. | campylobacter jejuni with gm1 ganglioside in the core of its lipopolysaccharide has been associated with guillain-barré syndrome. since this epitope may be of considerable pathophysiologic importance and since this ganglioside binds cholera toxin, a rapid screening assay to detect bacteria that bind cholera toxin as an indication of gm1 on their surfaces was developed. in the assay, bacterial lawns were grown on agar plates, harvested with phosphate-buffered saline, boiled, and incubated with a ... | 1998 | 9650959 |
| the central, surface-exposed region of the flagellar hook protein flge of campylobacter jejuni shows hypervariability among strains. | in a previous study, we observed that monoclonal antibodies raised against the hook protein flge of campylobacter jejuni lio 36, isolate 5226, bound exclusively to this strain. the aim of this study was to elucidate the molecular basis for these binding specificities. the hook protein-encoding gene flge of c. jejuni was cloned in escherichia coli and sequenced. the flge genes of four additional c. jejuni strains were amplified by pcr and also sequenced. comparison of the deduced amino acid seque ... | 1998 | 9658019 |
| antimicrobial susceptibility testing of 59 strains of campylobacter fetus subsp. fetus. | the susceptibilities of 59 campylobacter fetus subsp. fetus isolates to eight antibiotics were studied by the agar dilution, e-test, and disk diffusion methods. none of the isolates were beta-lactamase producers. all were susceptible to ampicillin, gentamicin, imipenem, and meropenem as determined by the three methods, with mics at which 90% of the isolates are inhibited (mic90s) (determined by agar dilution) of 2, 1, < or = 0.06, and 0.12 microgram/ml, respectively. twenty-seven percent of the ... | 1998 | 9661033 |
| campylobacter upsaliensis: waiting in the wings. | despite strong epidemiological evidence supporting an important role for campylobacter upsaliensis as a human enteropathogen, it remains relatively unknown in the realm of clinical microbiology. clinical studies indicate that infection with this organism usually is associated with benign self-limiting diarrhea. however, more serious illnesses, including spontaneous abortion and hemolytic-uremic syndrome, recently have been associated with human infections. understanding of the virulence properti ... | 1998 | 9665977 |
| campylobacter species and guillain-barré syndrome. | since the eradication of polio in most parts of the world, guillain-barré syndrome (gbs) has become the most common cause of acute flaccid paralysis. gbs is an autoimmune disorder of the peripheral nervous system characterized by weakness, usually symmetrical, evolving over a period of several days or more. since laboratories began to isolate campylobacter species from stool specimens some 20 years ago, there have been many reports of gbs following campylobacter infection. only during the past f ... | 1998 | 9665983 |
| etiological study of diarrheal patients in vientiane, lao people's democratic republic. | the etiological agents of diarrhea in vientiane, lao people's democratic republic (lao pdr), were studied in the period from october 1996 to august 1997. a total of 880 patients with diarrhea visiting medical facilities were examined for shigella, salmonella, diarrheagenic escherichia coli, vibrio, aeromonas, campylobacter, and rotavirus. shigella spp., heat-stable enterotoxin (st)-producing e. coli, and serogroup-based enteropathogenic e. coli were found to be the main organisms causing diarrhe ... | 1998 | 9665990 |
| associations between heat-stable (o) and heat-labile (hl) serogroup antigens of campylobacter jejuni: evidence for interstrain relationships within three o/hl serovars. | a comparative examination of the heat-stable (o) and heat-labile (hl) serogrouping results for 9,024 sporadic human isolates of campylobacter jejuni revealed conserved associations between specific o and hl antigens (o/hl serovars). forty-nine percent of the isolates which grouped for both o and hl antigens belonged to one of three serovars: o 4 complex/hl 1 (17.9%), o 1/hl 2 (16.8%), or o 50/hl 7 (14.5%). other common serovars were o 2/hl 4 (8.3%), o 6/hl 6 (8.1%), o 53/hl 11 (4.5%), o 19/hl 17 ... | 1998 | 9665996 |
| lipopolysaccharides from campylobacter jejuni o:41 strains associated with guillain-barré syndrome exhibit mimicry of gm1 ganglioside. | three campylobacter jejuni, biotype 2, serotype o:41 strains that were isolated from patients who developed guillain-barré syndrome (gbs) and one c. jejuni isolate from a patient who developed enteritis only were examined. the aim of the study was to determine the structure of the core oligosaccharide (os) of the lipopolysaccharide (lps) of c. jejuni serotype o:41, a serotype rarely associated with the development of gbs, and to determine if the lps shares similar epitopes with any of the major ... | 1998 | 9673245 |
| phospholipase a of yersinia enterocolitica contributes to pathogenesis in a mouse model. | some isolates of yersinia enterocolitica exhibit phospholipase activity, which has been linked to lecithin-dependent hemolysis (m. tsubokura, k. otsoki, i. shimohira, and h. yamamoto, infect. immun. 25:939-942, 1979). a gene encoding y. enterocolitica phospholipase was identified, and analysis of the nucleotide sequence revealed two tandemly transcribed open reading frames. the first, ypla, has 74% identity and 85% similarity to the phospholipase a found in serratia liquefaciens. though the othe ... | 1998 | 9673284 |
| incidence and clinical manifestations of campylobacter enteritis in central taiwan. | campylobacter species are recognized as a major cause of acute gastroenteritis in humans throughout the world and are increasingly associated with the development of guillain-barré syndrome. the age distribution of persons and the seasonal patterns of campylobacter infection are different in many developed and developing countries. the present study reports the investigation of the clinical and epidemiologic features of campylobacter infections in central taiwan. | 1998 | 9684510 |
| antimicrobial resistance of clinical strains of campylobacter jejuni subsp. jejuni isolated from 1985 to 1997 in quebec, canada. | the antimicrobial resistance of 158 campylobacter jejuni strains isolated from humans in quebec, canada, from 1995 to 1997 was compared to the resistance of 47 and 86 strains of c. jejuni isolated in 1985 and 1986 and in 1992 and 1993, respectively. of the 291 c. jejuni strains tested, no strain was resistant to erythromycin. compared to the c. jejuni strains isolated in 1985 and 1986, the c. jejuni strains isolated in 1992 and 1993 were more resistant to tetracycline (40.7 versus 19.1%, respect ... | 1998 | 9687415 |
| mammalian cytidine 5'-monophosphate n-acetylneuraminic acid synthetase: a nuclear protein with evolutionarily conserved structural motifs. | sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. the pattern of cell surface sialylation is species- and tissue-specific, is highly regulated during embryonic development, and changes with stages of differentiation. a prerequisite for the synthesis of sialylated glycoconjugates is the activated sugar-nucleotide cytidine 5'-monophosphate n-acetylneuraminic acid (cmp-neu5ac), which provides a substrate for golgi sialylt ... | 1998 | 9689047 |
| the lipopolysaccharide biosynthesis locus of campylobacter jejuni 81116. | most campylobacter jejuni strains express lipo-oligosaccharides. some strains also express lipopolysaccharides (lps), with o-antigen-like carbohydrate repeats. c. jejuni 81116 expresses an lps containing both lipo-oligosaccharides and o-antigen-like repeats, but nothing is known about the structure or sugar composition of these lps species. a cosmid library of the genome of c. jejuni 81116 was constructed and probed with campylobacter hyoilei genes involved in lps synthesis. five cosmids hybridi ... | 1998 | 9720026 |
| metronidazole resistance and microaerophily in campylobacter species. | metronidazole is active against most anaerobic organisms and is also used in the treatment of the microaerophilic bacterium helicobacter pylori. resistance to metronidazole is uncommon in most anaerobic organisms, but it is increasingly prevalent in h. pylori. previously we have suggested that metronidazole resistance in h. pylori is inherent in the microaerophilic nature of the organism and therefore would be present in other microaerophiles such as campylobacter. short periods of anaerobiosis ... | 1998 | 9732442 |
| in vitro genotypic variation of campylobacter coli documented by pulsed-field gel electrophoretic dna profiling: implications for epidemiological studies. | six isolates of campylobacter coli from different pig herds were subcultured up to 50 times over a 6-month period and dna samples suitable for pulsed-field gel electrophoretic (pfge) profiling prepared at regular (1, 20, 40 and 50 passages) intervals. in 5/6 strains, changes in the banding patterns of sma1, sal1 and/or bamh1 digests were observed. in one such strain the differences were considered artifactual. however, significant alterations in pfge profiles between subcultures of four strains ... | 1998 | 9742707 |
| resistance to antimicrobial agents used for animal therapy in pathogenic-, zoonotic- and indicator bacteria isolated from different food animals in denmark: a baseline study for the danish integrated antimicrobial resistance monitoring programme (danmap). | this study describes the establishment and first results of a continuous surveillance system of antimicrobial resistance among bacteria isolated from pigs, cattle and broilers in denmark. the three categories of bacteria tested were: 1) indicator bacteria (escherichia coli, enterococcus faecalis, enterococcus faecium), 2) zoonotic bacteria (campylobacter coli/jejuni, salmonella enterica, yersinia enterocolitica), and 3) animal pathogens (e. coli, staphylococcus aureus, coagulase-negative staphyl ... | 1998 | 9744762 |
| identification of campylobacter jejuni promoter sequences. | a promoterless lacz shuttle vector, which allowed screening of promoters by beta-galactosidase activity in campylobacter jejuni and escherichia coli, was developed. chromosomal dna fragments from c. jejuni were cloned into this vector; 125 of 1,824 clones displayed promoter activity in c. jejuni. eleven clones with strong promoter activity in c. jejuni were further characterized. their nucleotide sequences were determined, and the transcriptional start sites of the putative promoters in c. jejun ... | 1998 | 9457862 |
| effect of low temperatures on growth, structure, and metabolism of campylobacter coli sp10. | the effect of low temperatures on the survival, structure, and metabolism of campylobacter coli sp10, a virulent strain, was investigated. c. coli became nonculturable rapidly at 20 and 10 degree c and slightly later at 4 degrees c. incubation in a microaerobic atmosphere improved survival, but after day 8, campylobacters were detectable by direct-count procedures only. the increase in the number of coccoid cells was most pronounced at 37 degrees c but also was noticeable at 20 and 10 degrees c. ... | 1998 | 9464397 |
| extended survival and persistence of campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and -rrna staining. | in water microcosm experiments, the survival times of campylobacter isolates differed by up to twofold, as determined by culturing; this difference increased to fourfold when particular combinations of temperature and oxygenation were used. the mean survival times were much longer at 4 and 10 degrees c (202 and 176 h, respectively) than at 22 and 37 degrees c (43 and 22 h, respectively). the influence of anaerobiosis on survival time was less dramatic and differed considerably between isolates. ... | 1998 | 9464415 |
| serotyping scheme for campylobacter jejuni and campylobacter coli based on direct agglutination of heat-stable antigens. | campylobacter is now the most frequently reported cause of gastrointestinal disease in england and wales, yet few isolates are characterized beyond the genus level. the majority of isolates are campylobacter jejuni (90%), with most of the remainder being campylobacter coli. we describe an adaptation of the penner serotyping scheme in which passive hemagglutination has been replaced by detection of heat-stable antigens by direct bacterial agglutination; absorbed antisera are used where appropriat ... | 1998 | 9466737 |
| trna(arg) (fimu) and expression of sef14 and sef21 in salmonella enteritidis. | a tn10 insertion affecting sef14 fimbrial synthesis in salmonella enteritidis was located 13 bp upstream of a gene designated fimu. the 77-bp dna sequence of fimu from s. enteritidis was identical to that of fimu encoding trna(arg) (ucu) from salmonella typhimurium and 96% identical to that of the escherichia coli argu homolog. furthermore, the open reading frame adjacent to and overlapping the 3' end of fimu was similar to the prophage dlp12 integrase gene. the fimu-encoded transcript comigrate ... | 1998 | 9473037 |
| mutation in the peb1a locus of campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. | campylobacter jejuni is one of the leading causes of bacterial diarrhea throughout the world. we previously found that peb1 is a homolog of cluster 3 binding proteins of bacterial abc transporters and that a c. jejuni adhesin, cell-binding factor 1 (cbf1), if not identical to, contains peb1. a single protein migrating at approximately 27 to 28 kda was recognized by anti-cbf1 and anti-peb1. to determine the role that the operon encoding peb1 plays in c. jejuni adherence, peb1a, the gene encoding ... | 1998 | 9488379 |
| the pseudomonas aeruginosa flagellar cap protein, flid, is responsible for mucin adhesion. | mucin-specific adhesion of pseudomonas aeruginosa plays an important role in the initial colonization of this organism in the airways of cystic fibrosis patients. we report here that the flagellar cap protein, flid, participates in this adhesion process. a polar chromosomal insertional mutation in the p. aeruginosa flid gene made this organism nonadhesive to mucin in an in vitro mucin adhesion assay. the adhesive phenotype was restored by providing the flid gene alone on a multicopy plasmid, sug ... | 1998 | 9488388 |
| variation in flagellin genes and proteins of burkholderia cepacia. | the majority of isolates of burkholderia cepacia, an important opportunistic pathogen associated with cystic fibrosis, can be classified into two types on the basis of flagellin protein size. electron microscopic analysis indicates that the flagella of strains with the larger flagellin type (type i) are wider in diameter. flagellin genes representative of both types were cloned and sequenced to design oligonucleotide primers for pcr amplification of the central variable domain of b. cepacia flag ... | 1998 | 9495748 |
| helicobacter pylori porcdab and oordabc genes encode distinct pyruvate:flavodoxin and 2-oxoglutarate:acceptor oxidoreductases which mediate electron transport to nadp. | helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. previous studies (n. j. hughes, p. a. chalk, c. l. clayton, and d. j. kelly, j. bacteriol. 177:3953-3959, 1995) have indicated that the major routes for the generation of acetyl coenzyme a (acetyl-coa) and succinyl-coa are via pyruvate:flavodoxin oxidoreductase (por) and 2-oxoglutarate:acceptor oxidoreductase (oor), respectively. th ... | 1998 | 9495749 |
| transcription of ppk from acinetobacter sp. strain adp1, encoding a putative polyphosphate kinase, is induced by phosphate starvation. | polyphosphate kinase (ppk) catalyzes the formation of polyphosphate from atp. we cloned the ppk gene (2,073 bp) from acinetobacter sp. strain adp1; this gene encodes a putative polypeptide of 78.6 kda with extensive homology to polyphosphate kinase from escherichia coli and other bacteria. chromosomal disruption of ppk by inserting a transcriptionally fused lacz does not affect growth under conditions of phosphate limitation or excess. beta-galactosidase activity expressed from the single-copy p ... | 1998 | 9501429 |
| nucleotide sequence and characterization of cdra, a cell division-related gene of helicobacter pylori. | we identified cell division-related gene cdra in helicobacter pylori hpk5. the putative gene product, cdra, is a 367-amino-acid polypeptide that exhibited a high level of homology to conserved hypothetical atp-binding protein hp0066 of h. pylori 26695, except in the n-terminal region, and showed some similarity to the ftsk/spoiiie family proteins. we isolated a cdra-disrupted mutant by allelic exchange mutagenesis. because of the low transformation frequency, the possibility that a suppressing m ... | 1998 | 9748467 |
| identification of a gene encoding a methyl-accepting chemotaxis-like protein form campylobacter coli and its use in a molecular typing scheme for campylobacters. | using pcr amplification with degenerate primers, a gene (tlpa) form campylobacter coli encoding a putative 63x0 kda polypeptide which exhibited significant identity with bacterial methyl-accepting chemotaxis proteins (mcps) was identified. a mutant containing an inactivated copy of the tlpa a gene showed a wild-type chemotactic response to all of the chemo-attractants tested. a dna probe based on the highly conserved domain (hcd) of tlpa revealed the presence of multiple copies of genes encoding ... | 1998 | 9750306 |
| physiological activity of campylobacter jejuni far below the minimal growth temperature. | the behavior of campylobacter jejuni at environmental temperatures was examined by determining the physiological activities of this human pathogen. the minimal growth temperatures were found to be 32 and 31 degrees c for strains 104 and atcc 33560, respectively. both strains exhibited a sudden decrease in growth rate from the maximum to zero within a few degrees not only near the maximal growth temperature but also near the minimal growth temperature. this could be an indication that a temperatu ... | 1998 | 9758819 |
| effective recovery of bacterial dna and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens. | a dna-based, direct method for initial characterization of the total bacterial community in ileum and cecum of the chicken gastrointestinal (gi) tract was developed. the efficiencies of bacterial extraction and lysis were >95 and >99%, respectively, and therefore the dna recovered should accurately reflect the bacterial communities of the ileal and cecal digesta. total bacterial dna samples were fractionated according to their percent g+c content. the profiles reflecting the composition of the b ... | 1998 | 9758849 |
| use of a selective medium and a membrane filter method for isolation of campylobacter species from spanish paediatric patients. | a study was conducted to assess the value of a combination of two culture methods for isolation of campylobacter spp. from spanish children. seven hundred twenty-nine diarrhoeal stool specimens from 599 patients were examined for campylobacter spp. by culturing them on charcoal cefoperazone deoxycholate agar and on blood agar with a membrane filter. one hundred sixteen campylobacter strains were isolated from a total of 108 specimens; 75 (64.6%) were campylobacter jejuni, 32 (27.5%) were campylo ... | 1998 | 9764551 |
| iron-responsive gene regulation in a campylobacter jejuni fur mutant. | the expression of iron-regulated systems in gram-negative bacteria is generally controlled by the fur protein, which represses the transcription of iron-regulated promoters by using fe2+ as a cofactor. mutational analysis of the campylobacter jejuni fur gene was carried out by generation of a set of mutant copies of fur which had a kanamycin or chloramphenicol resistance gene introduced into the regions encoding the n and c termini of the fur protein. the mutated genes were recombined into the c ... | 1998 | 9765558 |
| [relapsing campylobacter coli bacteremia in a hypogammaglobulinemic patient]. | 1998 | 9767813 | |
| detection of point mutations associated with resistance of helicobacter pylori to clarithromycin by hybridization in liquid phase. | when the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as helicobacter pylori. treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on the 23s rrna gene. this resistance is currently found in organisms isolated from 0 to 15% of patients and jeopardizes the success of the treatment. we have designed a test involvin ... | 1998 | 9774580 |
| a reverse transcriptase polymerase chain reaction assay for the detection of thermophilic campylobacter spp. | a novel method was developed for the detection of thermophilic enteropathogenic campylobacters based on the detection of mrna using the reverse transcriptase polymerase chain reaction (rt-pcr). the rna extraction method, dnase treatment and rt-pcr assay were shown to be specific for mrna. the assay is specific for the thermophilic campylobacters campylobacter jejuni, campylobacter coli and campylobacter upsaliensis and restriction fragment length polymorphism (rflp) analysis of the 256 bp amplif ... | 1998 | 9778457 |
| deletion of the major proteolytic site of the helicobacter pylori cytotoxin does not influence toxin activity but favors assembly of the toxin into hexameric structures. | the helicobacter pylori cytotoxin is proteolytically cleaved at a flexible hydrophilic loop into two subunits. deletion of the loop sequences had no effect on biological activity of the toxin in the hela cell vacuolation assay but favored the organization of the protein into hexameric rather than heptameric structures. | 1998 | 9784571 |
| cloning, sequencing, and characterization of the lipopolysaccharide biosynthetic enzyme heptosyltransferase i gene (waac) from campylobacter jejuni and campylobacter coli. | campylobacter jejuni and campylobacter coli are common causes of gastrointestinal disease and a proportion of c. jejuni infections have been shown to be associated with the guillain-barré syndrome. the waac gene from campylobacter coli, involved in lipopolysaccharide core biosynthesis, was cloned by complementation of a heptose-deficient strain of salmonella typhimurium, as judged by novobiocin sensitivity, lipopolysaccharide (lps)-specific phage sensitivity, and polyacrylamide-resolved lipopoly ... | 1998 | 9831648 |
| high-level resistance to trimethoprim in clinical isolates of campylobacter jejuni by acquisition of foreign genes (dfr1 and dfr9) expressing drug-insensitive dihydrofolate reductases. | the pathogenic bacterium campylobacter jejuni has been regarded as endogenously resistant to trimethoprim. the genetic basis of this resistance was characterized in two collections of clinical isolates of c. jejuni obtained from two different parts of sweden. the majority of these isolates were found to carry foreign dfr genes coding for resistant variants of the dihydrofolate reductase enzyme, the target of trimethoprim. the resistance genes, found on the chromosome, were dfr1 and dfr9. in abou ... | 1998 | 9835491 |
| rapid emergence of high-level resistance to quinolones in campylobacter jejuni associated with mutational changes in gyra and parc. | quinolone resistance in clinical isolates of campylobacter jejuni in sweden increased more than 20-fold at the beginning of the 1990s. resistance to 125 microgram of ciprofloxacin per ml in clinical isolates was associated with chromosomal mutations in c. jejuni leading to a thr-86-ile substitution in the gyra product and a arg-139-gln substitution in the parc product. | 1998 | 9835526 |
| effect of low-osmolality nutrient media on growth and culturability of campylobacter species. | the growth and culturability of campylobacter jejuni nctc 11351 and other campylobacters were examined in media having different osmolalities at a range of temperatures (4, 25, and 42 degreesc). the medium osmolalities used ranged from the osmolality of full-strength nutrient medium (modified campylobacter broth having an osmolality of around 254 mosmol) down to 96 mosmol. the following two methods were used to produce media having different osmolalities: dilution of the nutrient medium with dis ... | 1998 | 9835543 |
| a case control study of potential enteric pathogens for calves raised in cow-calf herds. | a matched case control study was performed to describe the epidemiological features of potential enteric pathogens for calves reared in 53 cow-calf herds located in western switzerland. a total of 106 diarrhoeic calves and 126 healthy control calves were collected, all calves were less than 4 months old. faecal samples were analysed for presence of infectious agents related to calf diarrhoea including enterotoxigenic e. coli, verotoxin producing e. coli (vtec), campylobacter sp., yersinia sp., s ... | 1998 | 9852767 |
| diagnostic potentials of the activities of lectins with whole cells of three bacterial diarrheagenic agents: aeromonas, plesiomonas, campylobacter. | the reactiviteisofstrains ofplesionmona shigeloides, campylobater jejuni, campylobacter coli and aeromonas species with some lectins namely soyabean agglutinin (sba), ricinus communis agglutinin (rca), peanut agllutinin (pna), cancanavalin-a (con-a), lima bean agglutinin (lma) and abrin were investigated using the microtitre plate method. the results obtained show that all stains of a. hydrophila, a. caviae, a. sobria and p. shigelloides reacted very strongly with pna and sba. all strains of c. ... | 1998 | 17581001 |
| short-sequence dna repeats in prokaryotic genomes. | short-sequence dna repeat (ssr) loci can be identified in all eukaryotic and many prokaryotic genomes. these loci harbor short or long stretches of repeated nucleotide sequence motifs. dna sequence motifs in a single locus can be identical and/or heterogeneous. ssrs are encountered in many different branches of the prokaryote kingdom. they are found in genes encoding products as diverse as microbial surface components recognizing adhesive matrix molecules and specific bacterial virulence factors ... | 1998 | 9618442 |
| amplified-fragment length polymorphism analysis: the state of an art. | 1999 | 10488158 | |
| nomenclature for new tetracycline resistance determinants. | letters of the english alphabet have heretofore been used to name tetracycline resistance determinants. since all 26 letters have now been used, a nomenclature employing numerals is recommended for future determinants, and one laboratory has offered to coordinate the assignment of numerals. | 1999 | 10348788 |
| rapid detection of the chlamydiaceae and other families in the order chlamydiales: three pcr tests. | few identification methods will rapidly or specifically detect all bacteria in the order chlamydiales, family chlamydiaceae. in this study, three pcr tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. two tests exclusively recognized the chlamydiaceae: a multiplex test targeting the ompa gene and the rrna intergenic spacer and a taqman test targeting the 23s ribosomal dna. the multiplex test was able to detect as few as 200 inclusion ... | 1999 | 9986815 |
| antiseptics and disinfectants: activity, action, and resistance. | antiseptics and disinfectants are extensively used in hospitals and other health care settings for a variety of topical and hard-surface applications. a wide variety of active chemical agents (biocides) are found in these products, many of which have been used for hundreds of years, including alcohols, phenols, iodine, and chlorine. most of these active agents demonstrate broad-spectrum antimicrobial activity; however, little is known about the mode of action of these agents in comparison to ant ... | 1999 | 9880479 |
| prevalence of campylobacter spp isolated from the intestinal tract of pigs raised in an integrated swine production system. | to enumerate the prevalence of campylobacter isolates in the intestinal tract of market-weight swine raised in an integrated swine operation in texas. | 1999 | 14567422 |
| flagellin gene polymorphism analysis of campylobacter compared with antigen serotyping. | flagellin gene sequence polymorphisms were used to discriminate amongst 53 strains of campylobacter jejuni and c. coli. the campylobacter strains were made up of forty-three strains of campylobacter jejuni and 10 strains of campylobacter coli. the results were analysed in relation to penner serotyping. twenty dna pcr-rflp patterns (genotypes) were identified by analysis of dde i fragment length polymorphisms in flagellin gene (fla a and fla b) polymerase chain reaction (pcr) products. flagellin ... | 1999 | 10627993 |
| [acute diarrhea in children less than 3 years of age: enteropathogens isolated in patient's stools, compared with a control group] | objectives: to observe the occurrence of different etiological agents of acute diarrhea (ad) in stool specimens of patients and children in a control group. material and methods: 100 children less than three years of age with ad were studied as well as 100 controls, between november 1993 and may 1994. stool specimens were collected in both groups and the following enteropathogens were searched for: rotavirus, escherichia coli (epec, etec, eiec, ehec), salmonella sp, shigella sp, campylobacter je ... | 1999 | 14685548 |
| studies on enteric campylobacteriosis in tikur anbessa and ethio-swedish children's hospital, addis ababa, ethiopia. | between february 1992 and january 1993 different campylobacter spp. were isolated from 86 (13.7%) of 630 patients with diarrhoea at tikur anbessa and ethio-swedish children's hospital, addis ababa, ethiopia. in the same study population, shigella spp. were found in 11.7% and salmonella spp. in 3.8%. campylobacter spp. were found in all age groups, but the majority were isolated from children less than five years of age (68.6%). only 2 (0.9%) of 220 controls had campylobacter in their stools. of ... | 1999 | 11957308 |
| passive immunity against human pathogens using bovine antibodies. | 1999 | 10337007 | |
| high-resolution genotyping of campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting. | for epidemiological studies of campylobacter infections, molecular typing methods that can differentiate campylobacters at the strain level are needed. in this study we used a recently developed genotyping method, amplified fragment length polymorphism (aflp), which is based on selective amplification of restriction fragments of chromosomal dna, for genetic typing of campylobacter jejuni and campylobacter coli strains derived from humans and poultry. we developed an automated aflp fingerprinting ... | 1999 | 10347015 |
| generation of a superoxide dismutase (sod)-deficient mutant of campylobacter coli: evidence for the significance of sod in campylobacter survival and colonization. | the microaerophilic nature of campylobacter species implies an inherent sensitivity towards oxygen and its reduction products, particularly the superoxide anion. the deleterious effects of exposure to superoxide radicals are counteracted by the activity of superoxide dismutase (sod). we have shown previously that campylobacter coli possesses an iron cofactored sod. the sodb gene of c. coli ua585 was insertionally inactivated by the site-specific insertion of a teto cassette. organisms harboring ... | 1999 | 10347040 |
| evaluation of the anaeropack campylo system for growth of microaerophilic bacteria. | growth of microaerophilic bacteria in the anaeropack campylo (mitsubishi gas chemical america, inc., new york, n.y.) atmosphere generation system was compared to growth in the campypak plus jar and campypak pouch (becton-dickinson microbiology systems [bdms], cockeysville, md.). growth in the anaeropack campylo system was considered equivalent to or better than growth obtained in the campypak plus and campypak pouch systems for 48 of the 50 helicobacter pylori strains and for all 28 campylobacte ... | 1999 | 10364622 |
| campylobacter coli strains with enlarged flagellin genes isolated from river water. | a group of campylobacters isolated from river water were found to possess unusually large flagellin genes. both phenotype and serology were consistent with identification as campylobacter coli. phylogenetic analysis of small (16s, rrs) and large subunit (23s, rrl) rrna genes of a representative strain, nctc 13006, demonstrated high levels of relatedness with c. jejuni and c. coli (99.1 and 98.3% similarity for 16s; 99.3 and 99.4% similarity for 23s). large flagellin proteins were demonstrated by ... | 1999 | 10376486 |
| in-vitro activity of moxifloxacin against fluoroquinolone-resistant strains of aerobic gram-negative bacilli and enterococcus faecalis. | mics of the new fluoroquinolone, moxifloxacin, and those of ciprofloxacin, ofloxacin and sparfloxacin for 19 genetically characterized fluoroquinolone-resistant strains were determined by the agar dilution method. the mics of moxifloxacin for escherichia isolates with one mutation in gyra (corresponding to ser83-->leu or asp87-->gly substitution) were 0.25-0.5 mg/l, while those of ciprofloxacin, ofloxacin and sparfloxacin were 0.06-0.25, 1 and 0.12-0.5 mg/l, respectively. these values were four- ... | 1999 | 10382871 |
| evaluation of the effect of temperature and nutrients on the survival of campylobacter spp. in water microcosms. | batch microcosms containing various water types (de-ionized and river water with or without sediment), incubated at a range of temperatures (5-37 degrees c), were used to facilitate a comparative evaluation of the significance of such variables and their interactions upon the collective and individual survival of four species of thermophilic campylobacter. all variables significantly influenced (p < = 0.031) population decay rates. minimal decay for the group was identified at low temperatures ( ... | 1999 | 10389249 |
| rapid detection, by pcr and reverse hybridization, of mutations in the helicobacter pylori 23s rrna gene, associated with macrolide resistance. | a pcr-based reverse hybridization system (research prototype kit inno-lipa for h. pylori resistance) was developed and evaluated for simultaneous detection of 23s ribosomal dna point mutations, associated with macrolide resistance in helicobacter pylori. fifty-seven h. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by pcr-lipa (detecting mutations a2115-->g, g2141-->a, a2142-->g, a2142-->c, a2143-->g, a2143-->c, and a2143-->t), d ... | 1999 | 10390244 |
| identification of a glycoprotein produced by enterotoxigenic escherichia coli. | enterotoxigenic escherichia coli (etec) strain h10407 is capable of invading epithelial cell lines derived from the human ileocecum and colon in vitro. two separate chromosomally encoded invasion loci (tia and tib) have been cloned from this strain. these loci direct nonadherent and noninvasive laboratory strains of e. coli to adhere to and invade cultured human intestinal epithelial cells. the tib locus directs the synthesis of tiba, a 104-kda outer membrane protein that is directly correlated ... | 1999 | 10417177 |
| campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of human intestinal cells. | campylobacter jejuni uptake into cultured int407 cells was analyzed kinetically over a wide range of starting multiplicities of infection (moi; from 0.02 to 20,000 bacteria/epithelial cell). the efficiency of internalization was the highest at moi of 0.02 and decreased steadily at higher mois, presumably due to reported c. jejuni autoagglutination at higher densities. total internalized campylobacter cfu increased gradually from an moi of 0.02 to a peak at an moi of 200 (reaching an average of t ... | 1999 | 10417189 |
| occurrence, removal and seasonal variation of thermophilic campylobacters and arcobacter in sewage sludge. | the presence of thermophilic campylobacters and arcobacter was investigated in four types of sewage sludge taken from the treatment plant in bologna (italy): primary, activated, thickened and anaerobically digested sludge. campylobacter jejuni and campylobacter coli were more numerous during the march-september period and were found only in primary sludge (22.7%) with mean counts of 278 mpn/g dry matter and 1403 mpn/g dry matter respectively. arcobacter butzleri were found in all types of sludge ... | 1999 | 10418097 |
| incidence of salmonella, campylobacter jejuni, campylobacter coli, and listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the belgian retail market. | from january 1997 to may 1998, 772 samples of poultry carcasses and poultry products for sale on the retail market in belgium were analyzed for the presence of salmonella spp., salmonella enteritidis, campylobacter jejuni, c. coli, and listeria monocytogenes per 100 cm2 or 25 g. poultry samples were contaminated with salmonella (36.5%), c. jejuni and c. coli (28.5%), and l. monocytogenes (38.2%). in about 12.3% of the poultry samples, the l. monocytogenes contamination level exceeded 1 cfu per g ... | 1999 | 10419264 |
| an iron-regulated alkyl hydroperoxide reductase (ahpc) confers aerotolerance and oxidative stress resistance to the microaerophilic pathogen campylobacter jejuni. | microaerophiles like campylobacter jejuni must resist oxidative stresses during transmission or infection. growth of c. jejuni 81116 under iron limitation greatly increased the expression of two polypeptides of 26 and 55 kda. the identification of these proteins by n-terminal amino acid sequencing showed both to be involved in the defense against oxidative stress. the 55-kda polypeptide was identical to c. jejuni catalase (kata), whereas the n terminus of the 26-kda polypeptide was homologous to ... | 1999 | 10438747 |
| direct analysis and identification of helicobacter and campylobacter species by maldi-tof mass spectrometry. | campylobacter jejuni, campylobacter fetus, and campylobacter coli were compared with helicobacter pylori and helicobacter mustelae by direct analysis of individual cultured colonies in 50% methanol-water with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (maldi-tof ms). h. pylori and campylobacter species from blood agar culture produced unique, complex spectra with over 25 different ions in mass/charge (m/z) range from 2,000 to 62,000. a biomarker for h. pylori ... | 1999 | 10464475 |
| sulfonamide resistance in clinical isolates of campylobacter jejuni: mutational changes in the chromosomal dihydropteroate synthase. | the characterization of the genetic basis of sulfonamide resistance in campylobacter jejuni was attempted. the resistance determinant from a sulfonamide-resistant strain of c. jejuni was cloned and was found to show 42% identity with the folp gene (which codes for dihydropteroate synthase, the target of sulfonamides) of the related bacterium helicobacter pylori. the sequences of the areas surrounding the folp gene in c. jejuni showed similarity to those of the areas surrounding the corresponding ... | 1999 | 10471557 |
| metabolism and genetics of helicobacter pylori: the genome era. | the publication of the complete sequence of helicobacter pylori 26695 in 1997 and more recently that of strain j99 has provided new insight into the biology of this organism. in this review, we attempt to analyze and interpret the information provided by sequence annotations and to compare these data with those provided by experimental analyses. after a brief description of the general features of the genomes of the two sequenced strains, the principal metabolic pathways are analyzed. in particu ... | 1999 | 10477311 |
| hp0333, a member of the dpra family, is involved in natural transformation in helicobacter pylori. | helicobacter pylori is naturally competent for dna transformation, but the mechanism by which transformation occurs is not known. for haemophilus influenzae, dpra is required for transformation by chromosomal but not plasmid dna, and the complete genomic sequence of h. pylori 26695 revealed a dpra homolog (hp0333). examination of genetic databases indicates that dpra homologs are present in a wide variety of bacterial species. to examine whether hp0333 has a function similar to dpra of h. influe ... | 1999 | 10482496 |
| the c-terminal domain of the bordetella pertussis autotransporter brka forms a pore in lipid bilayer membranes. | brka is a 103-kda outer membrane protein of bordetella pertussis that mediates resistance to antibody-dependent killing by complement. it is proteolytically processed into a 73-kda n-terminal domain and a 30-kda c-terminal domain as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. brka is also a member of the autotransporter family of proteins. translocation of the n-terminal domain of the protein across the outer membrane is hypothesized to occur through a pore formed by ... | 1999 | 10482528 |
| recombinant single-chain fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization. | using phage-display technology, a recombinant single-chain fv antibody fragment (scfv) was rapidly generated from the k16-16 hybridoma secreting mouse monoclonal antibody (mab) that binds to acetylaminofluorene-labeled dna (aaf-dna). the selected a4 phage-scfv specifically bound to aaf-dna. the anti-aaf scfv gene was then recloned into a fusion vector for the production of a hybrid protein comprising the antibody fragment fused to a potent bacterial alkaline phosphatase variant (phoav). the anti ... | 1999 | 10485265 |
| phage typing of campylobacter jejuni and campylobacter coli and its use as an adjunct to serotyping. | campylobacter is the most commonly reported cause of gastro-intestinal infection in england and wales, with over 50,000 reported cases in 1997. the majority of human campylobacter isolates in england and wales are c. jejuni (c. 90%) with most of the remainder being c. coli. we describe the use of phage typing as an extension to serotyping for more detailed characterization within these two species. the scheme was piloted during a study of 2407 c. jejuni and 182 c. coli strains isolated in wales ... | 1999 | 10487641 |
| ciprofloxacin resistance in campylobacter jejuni isolates: detection of gyra resistance mutations by mismatch amplification mutation assay pcr and dna sequence analysis. | the gyra quinolone resistance determining region was sequenced from 13 ciprofloxacin-resistant and 20 ciprofloxacin-susceptible campylobacter jejuni isolates. all isolates resistant to ciprofloxacin had thr-86-to-ile mutations, a mutation frequently associated with the acquisition of resistance to fluoroquinolones. a mismatch amplification mutation assay (mama) pcr protocol was developed that detects this gyra mutation in quinolone-resistant isolates. the mama pcr provides a means for routine de ... | 1999 | 10488192 |
| specific pcr identification and differentiation of the thermophilic campylobacters, campylobacter jejuni, c. coli, c. lari, and c. upsaliensis. | a sensitive pcr assay that detects the thermophilic campylobacters c. jejuni, c. coli, c. lari, and c. upsaliensis is reported. furthermore, by digestion of the pcr products with two restriction enzymes, species differentiation was demonstrated. thus, the present method has the potential to be used for both detection and identification of thermophilic campylobacter species. | 1999 | 10488210 |
| the absence of cecal colonization of chicks by a mutant of campylobacter jejuni not expressing bacterial fibronectin-binding protein. | campylobacter jejuni is a common cause of human gastrointestinal illness throughout the world. infections with c. jejuni and campylobacter coli are frequently acquired by eating undercooked chicken. the ability of c. jejuni to become established in the gastrointestinal tract of chickens is believed to involve binding of the bacterium to the gastrointestinal surface. a 37-kd outer membrane protein, termed cadf, has been described that facilitates the binding of campylobacter to fibronectin. this ... | 1999 | 10494431 |
| phase variation in helicobacter pylori lipopolysaccharide due to changes in the lengths of poly(c) tracts in alpha3-fucosyltransferase genes. | the lipopolysaccharide (lps) of helicobacter pylori expresses the lewis x (lex) and/or ley antigen. we have shown previously that h. pylori lps displays phase variation whereby an lex-positive strain yields variants with different lps serotypes, for example, lex plus ley or nonfucosylated polylactosamine. h. pylori has two alpha3-fucosyltransferase genes that both contain poly(c) tracts. we now demonstrate that these tracts can shorten or lengthen randomly, which results in reversible frameshift ... | 1999 | 10496917 |
| viability and dna maintenance in nonculturable spiral campylobacter jejuni cells after long-term exposure to low temperatures. | survival of campylobacter jejuni at 4 and 20 degrees c was investigated by using cellular integrity, respiratory activity, two-dimensional (2d) protein profile, and intact dna content as indicators of potential viability of nonculturable cells. intact dna content after 116 days, along with cellular integrity and respiring cells, was detected for up to 7 months at 4 degrees c by pulsed-field gel electrophoresis. most changes in 2d protein profiles involved up- or down-regulation. | 1999 | 10508106 |
| campylobacter jejuni contains two fur homologs: characterization of iron-responsive regulation of peroxide stress defense genes by the perr repressor. | expression of the peroxide stress genes alkyl hydroperoxide reductase (ahpc) and catalase (kata) of the microaerophile campylobacter jejuni is repressed by iron. whereas iron repression in gram-negative bacteria is usually carried out by the fur protein, previous work showed that this is not the case in c. jejuni, as these genes are still iron repressed in a c. jejuni fur mutant. an open reading frame encoding a fur homolog (designated perr for "peroxide stress regulator") was identified in the ... | 1999 | 10515927 |
| routine identification of campylobacter jejuni and campylobacter coli from human stool samples. | correct identification of campylobacter jejuni and campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. the widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are not generally available. this investigation was performed to assess the performance of common biochemical identification in comparison with species-specific pcr and gas liquid chro ... | 1999 | 10518720 |
| evaluation of a truncated recombinant flagellin subunit vaccine against campylobacter jejuni. | a recombinant protein comprising the maltose-binding protein (mbp) of escherichia coli fused to amino acids 5 to 337 of the flaa flagellin of campylobacter coli vc167 was evaluated for immunogenicity and protective efficacy against challenge by a heterologous strain of campylobacter, campylobacter jejuni 81-176, in two murine models. the sequence of the flaa gene of strain 81-176 revealed a predicted protein which was 98.1% similar to that of vc167 flaa over the region expressed in the fusion pr ... | 1999 | 10531231 |
| molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide o antigen of escherichia coli serotype o113. | shiga toxigenic escherichia coli (stec) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. within the stec family, eae-positive stec strains, particularly those belonging to serogroups o157 and o111, appear to have greater virulence for humans. however, in spite of being eae negative, stec strains belonging to serogroup o113 have frequently been associated with cases of severe stec disease, including hemolytic-uremic syndrome (hus). western blo ... | 1999 | 10531250 |
| recovery in embryonated eggs of viable but nonculturable campylobacter jejuni cells and maintenance of ability to adhere to hela cells after resuscitation. | the existence of a viable but nonculturable (vbnc) state has been described for campylobacter jejuni as it had been for a number pathogenic bacteria. three c. jejuni human isolates were suspended in surface water and subsequently entered the vbnc state. after starvation for 30 days, vbnc cells were inoculated in the yolk sacs of embryonated eggs. culturable cells were detected in a large proportion of the embryonated eggs inoculated with vbnc c. jejuni cells. recovered cells kept their adhesion ... | 1999 | 10543837 |