Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| rapid, simple, quantitative, and highly sensitive antibody detection for lyme disease. | there is currently a need for improved serological tests for the diagnosis and monitoring of lyme disease, an infection caused by borrelia burgdorferi. in the present study, we evaluated luciferase immunoprecipitation systems (lipss) for use for profiling of the antibody responses to a panel of b. burgdorferi proteins for the diagnosis of lyme disease. initially, serum samples from a cohort of patients and controls (n = 46) were used for training and were profiled by the use of 15 different b. b ... | 2010 | 20392886 |
| a 46-year-old woman with chin pain and a fainting spell. | 2010 | 20391493 | |
| 'one health', from science to policy: examples from the israeli experience. | 'the concept of 'one health' aims to help bring about the integration of animal, human and environmental health for the mutual benefit of all. this multidisciplinary approach is of great importance in a variety of fields, from enhancement of breakthroughs in biomedical research, to epidemiological studies and public health policy decision-making. we demonstrate the strength embedded in this approach by using three case studies from israel. we first describe successful scientific collaboration be ... | 2009 | 20391389 |
| induction of distinct neurologic disease manifestations during relapsing fever requires t lymphocytes. | relapsing fever borreliosis is a multisystemic infection characterized primarily by bacteremia but can extend to the cns. the incidence of cns disease manifestations in humans depends on the infecting relapsing fever borrelia species. in the murine model of borrelia hermsii infection we found high incidence of distinct signs of cns disease that ranged from a flaccid tail to complete paralysis of hind limbs. infiltration of large number of t cells into the spinal cord of b. hermsii-infected mice ... | 2010 | 20382883 |
| population genetics of amblyomma americanum (acari: ixodidae) collected from arkansas. | lone star ticks, amblyomma americanum l. (acari: ixodidae),infest multiple hosts such as birds, and mammals of various sizes (rodents to white-tailed deer) and can harbor human pathogens such as borrelia lonestari and ehrlichiosis chaffeensis. the population structure of 251 a. americanum ticks, collected from canines and two white-tailed deer in six arkansas ecoregions, was examined using dna sequences of a 247-bp region of the mitochondrial dna ribosomal rna 16s gene. of the 247 nucleotide cha ... | 2010 | 20380295 |
| community ecology and disease risk: lizards, squirrels, and the lyme disease spirochete in california, usa. | vector-borne zoonotic diseases are often maintained in complex transmission cycles involving multiple vertebrate hosts and their arthropod vectors. in the state of california, u.s.a., the spirochete borrelia burgdorferi, which causes lyme disease, is transmitted between vertebrate hosts by the western black-legged tick, ixodes pacificus. several mammalian species serve as reservoir hosts of the spirochete, but levels of tick infestation, reservoir competence, and borrelia-infection prevalence va ... | 2010 | 20380218 |
| imported and travelling dogs as carriers of canine vector-borne pathogens in germany. | abstract: | 2010 | 20377872 |
| comparison of selected canine vector-borne diseases between urban animal shelter and rural hunting dogs in korea. | abstract: a serological survey for dirofilaria immitis, anaplasma phagocytophilum, ehrlichia canis, and borrelia burgdorferi infections in rural hunting and urban shelter dogs mainly from southwestern regions of the republic of korea (south korea) was conducted. from a total of 229 wild boar or pheasant hunting dogs, the number of serologically positive dogs for any of the four pathogens was 93 (40.6%). the highest prevalence observed was d. immitis (22.3%), followed by a. phagocytophilum (18.8% ... | 2010 | 20377869 |
| direct detection of borrelia burgdorferi spirochetes in patients with early disseminated lyme borreliosis. | the detection of spirochetes in 15 patients with clinically documented early disseminated lb has been analysed when using cultivation method of the plasma or the cerebrospinal fluid, electron microscopy, commercial western blot and detecting the dna of the pathogen in vitro cultures by pcr-rflp. spirochetes were isolated in eight blood and one cerebrospinal fluid culture samples. in seven cases (47%), previous serodiagnostic laboratory tests were negative. borrelial dna was detected by pcr in 67 ... | 2009 | 20377044 |
| infectious arthritis and immune dysregulation: lessons from lyme disease. | borrelia burgdorferi colonization of the joints induces an inflammatory response, which in some individuals progresses to chronic arthritis. in this review, we discuss novel pathways that are implicated in disease development by modulating host defenses to b. burgdorferi infection. | 2010 | 20375899 |
| [presence of antibodies against gangliosides among patients with lyme borreliosis--preliminary study]. | the aim of the study was the evaluation of autoantibody reaction against endogenous gangliosides in the course of lyme borreliosis. | 2010 | 20369737 |
| [coexisting of borreliosis, depression and psoriasis--case report]. | lyme disease is a multisystem infectious disease with a wide variety of symptoms involving the skin as well as the nervous system. lyme disease is caused by spirochaete borrelia burgdorferi transmitted by ixodes ticks in endemic regions. a case of 45 year old woman suffering from borreliosis, psoriasis and depression is presented in the paper. in the study a standardized screening list was applied to evaluate the psychiatric health state--classification of psychic disorders (dsm-iv) and hamilton ... | 2010 | 20369726 |
| the bba64 gene of borrelia burgdorferi, the lyme disease agent, is critical for mammalian infection via tick bite transmission. | the spirochetal agent of lyme disease, borrelia burgdorferi, is transmitted by bites of ixodes ticks to mammalian reservoir hosts and humans. the mechanism(s) by which the organism is trafficked from vector to host is poorly understood. in this study, we demonstrate that a b. burgdorferi mutant strain deficient in the synthesis of the bba64 gene product was incapable of infecting mice via tick bite even though the mutant was (i) infectious in mice when introduced by needle inoculation, (ii) acqu ... | 2010 | 20368453 |
| [genotyping some borrelia burgdorferi sensu lato isolates from ixodes ricinus ticks in russia and ukraine]. | the 4 borrelia burgdorferi sensu lato isolates obtained from 1. ricinus ticks collected in the natural foci in russia and ukraine, having an unusual rflp msel-pattern, were studied using sequencing rrfa-rrlb spacer and rrs gene. the ir-5215 isolate from the tick collected in southern ukraine represented recently described genospecies b. spielmanii pathogenic for humans. the three atypical isolates ir-3519, ir-4721, and ir-4812 had 100% identity with the sequence of the atypical european b. burgd ... | 2010 | 20364477 |
| 31-year-old man with fever, palpitations, and generalized rash. | 2010 | 20360288 | |
| outcomes of children treated for lyme arthritis: results of a large pediatric cohort. | children often develop arthritis secondary to lyme disease; however, optimal treatment of lyme arthritis in pediatric patients remains ill-defined. we sought to characterize the outcomes of a large cohort of children with lyme arthritis treated using the approach recommended by the american academy of pediatrics and the infectious diseases society of america. | 2010 | 20360182 |
| disordered lymphoid purine metabolism contributes to the pathogenesis of persistent borrelia garinii infection in mice. | extracellular atp and adenosine are important regulators of immune responses; however, contribution of purinergic signaling to host defense during persistent microbial infections remains obscure. lyme borreliosis is a common arthropod-borne infection caused by borrelia burgdorferi sensu lato. in this study, we investigated whether lymphoid purinergic signaling contributes to the mechanisms by which borreliae species evade the immune system and trigger joint inflammation. intracutaneous inoculati ... | 2010 | 20357256 |
| horizontal and vertical movements of host-seeking ixodes pacificus (acari: ixodidae) nymphs in a hardwood forest. | the nymph of the western black-legged tick (ixodes pacificus) is an important bridging vector of the lyme disease spirochete (borrelia burgdorferi) to humans in the far-western united states. the previously unknown dispersal capabilities of this life stage were studied in relation to logs, tree trunks, and adjacent leaf-litter areas in a mixed hardwood forest using mark-release-recapture methods. in two spatially and temporally well-spaced trials involving logs, the estimated mean distances that ... | 2009 | 20352083 |
| phagocytosis of the lyme disease spirochete, borrelia burgdorferi, by cells from the ticks, ixodes scapularis and dermacentor andersoni, infected with an endosymbiont, rickettsia peacockii. | tick cell lines were used to model the effects of endosymbiont infection on phagocytic immune responses. the lines tested for their ability to phagocytose the lyme disease spirochete, borrelia burgdorferi (spirochaetales: spirochaetaceae), were ise6 and ide12 from the black-legged tick, ixodes scapularis say (acari: ixodidae) and dae15 from the rocky mountain wood tick, dermacentor andersoni stiles. rickettsia peacockii (rickettsiales: rickettsiaceae), an endosymbiont of d. andersoni, was used a ... | 2007 | 20331397 |
| borrelia carolinensis sp. nov., a novel species of the borrelia burgdorferi sensu lato complex isolated from rodents and a tick from the south-eastern usa. | a group of 16 isolates with genotypic characteristics different from those of known species of the borrelia burgdorferi sensu lato complex were cultured from ear biopsies of the rodents peromyscus gossypinus and neotoma floridana trapped at five localities in south carolina, usa, and from the tick ixodes minor feeding on n. floridana. multilocus sequence analysis of members of the novel species, involving the 16s rrna gene, the 5s-23s (rrf-rrl) intergenic spacer region and the flagellin, ospa an ... | 2011 | 20305062 |
| [causes and diagnostics of dizziness of central origin in children]. | dizziness and vertigo are symptoms caused by several etiological factors, they are result of general diseases, within anemia. they are associated with several diseases of the organ of hearing and balance, which are under laryngological care, and one of most important tests to distinguish vertigo of central and peripheral origin is videonystagmography. several diseases of the central nervous system cause instability of posture and gait. psychogenic feeling of being insecure and unsafe makes the d ... | 2009 | 20297643 |
| agglutination studies of the smaller oral treponemes, borrelia vincenti and cultured strains of treponema pallidum. | 1947 | 20292512 | |
| preservation of borrelia vincenti and cultured strains of treponema pallidum by the lyophil process. | 1947 | 20285099 | |
| borrelia tonsillitis: common symptoms but uncommon organism. | we present a severe case of acute tonsillitis caused by borrelia vincenti. b. vincenti does not only cause plaut-vincent's angina, but also a more localised infection of the tonsil. | 2010 | 20237790 |
| one-year duration of immunity induced by vaccination with a canine lyme disease bacterin. | laboratory-reared beagles were vaccinated with a placebo or a bacterin comprised of borrelia burgdorferi s-1-10 and ospa-negative/ospb-negative b. burgdorferi 50772 and challenged after 1 year with b. burgdorferi-infected ixodes scapularis ticks. for the placebo recipients, spirochetes were recovered from 9 (60%) skin biopsy specimens collected after 1 month, and the organisms persisted in the skin thereafter. ten (67%) dogs also developed joint infection (3 dogs), lameness or synovitis (7 dogs) ... | 2010 | 20237200 |
| first report of lyme disease spirochetes in ticks from romania (sibiu county). | we examined 200 questing ixodes ricinus ticks (nymphs and adults) collected in three different sites in sibiu county, romania by polymerase chain reaction (pcr) followed by reverse line blot (rlb) for borrelia burgdorferi s.l.. we detected the bacteria in 19% of the investigated ticks. the prevalence of infection was higher in nymphs (27%) than in adults (12%). two b. burgdorferi sensu lato genospecies were detected: b. garinii (20%) and b. afzelii (80%). we did not detect any mixed infections i ... | 2010 | 20232115 |
| increased sensitivity and specificity of borrelia burgdorferi 16s ribosomal dna detection. | the dna of borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (pcr) amplification of the species-specific 16s ribosomal dna (rdna). the primers were those well known to be specific for signature sequence amplification of the b burgdorferi sensu lato 16s ribosomal rna gene. the positive 293-base-pair nested pcr amplicon was subjected to routine direct automated sanger sequencing. a 50-base sequence excised randomly from t ... | 2010 | 20231610 |
| use of the cre-lox recombination system to investigate the lp54 gene requirement in the infectious cycle of borrelia burgdorferi. | borrelia burgdorferi, the causative agent of lyme disease, has a complex genome consisting of a linear chromosome and up to 21 linear and circular plasmids. these plasmids encode numerous proteins critical to the spirochete's infectious cycle and many hypothetical proteins whose functions and requirements are unknown. the conserved linear plasmid lp54 encodes several proteins important for survival in the mouse-tick infectious cycle, but the majority of the proteins are of unknown function and l ... | 2010 | 20231410 |
| molecular characterization of the interaction of borrelia parkeri and borrelia turicatae with human complement regulators. | in north america, tick-borne relapsing fever is caused by the species borrelia hermsii, b. parkeri, and b. turicatae, which are transmitted to humans through the bite of the respective infected tick vectors. here we describe the identification and functional characterization of a surface lipoprotein of b. parkeri, designated bpca, that binds the human complement regulators factor h and factor h-related protein 1 and, simultaneously, the host protease plasminogen. in contrast, the homologous b. t ... | 2010 | 20231403 |
| conventional and molecular diagnostic testing for the acute neurologic patient. | the aim of this review is to describe and evaluate both conventional and molecular diagnostic testing utilized in dogs and cats with acute neurologic diseases. various types of polymerase chain reaction (pcr) are explored along with novel molecular diagnostic testing that ultimately may prove useful in the critical care setting. | 2010 | 20230434 |
| invasion of the lyme disease vector ixodes scapularis: implications for borrelia burgdorferi endemicity. | lyme disease risk is increasing in the united states due in part to the spread of blacklegged ticks ixodes scapularis, the principal vector of the spirochetal pathogen borrelia burgdorferi. a 5-year study was undertaken to investigate hypothesized coinvasion of i. scapularis and b. burgdorferi in lower michigan. we tracked the spatial and temporal dynamics of the tick and spirochete using mammal, bird, and vegetation drag sampling at eight field sites along coastal and inland transects originati ... | 2010 | 20229127 |
| an intravascular immune response to borrelia burgdorferi involves kupffer cells and inkt cells. | here we investigate the dynamics of the hepatic intravascular immune response to a pathogen relevant to invariant natural killer t cells (inkt cells). immobilized kupffer cells with highly ramified extended processes into multiple sinusoids could effectively capture blood-borne, disseminating borrelia burgdorferi, creating a highly efficient surveillance and filtering system. after ingesting b. burgdorferi, kupffer cells induced chemokine receptor cxcr3-dependent clustering of inkt cells. kupffe ... | 2010 | 20228796 |
| safety of intravenous antibiotic therapy in patients referred for treatment of neurologic lyme disease. | although intravenous antibiotic therapy is recommended for neurologic lyme disease, safety concerns have been raised about treatment beyond 30 days in patients with persistent neurologic symptoms. the goal of our study was to evaluate the safety of extended intravenous antibiotic therapy in patients referred for treatment of neurologic lyme disease. | 2010 | 20228716 |
| fatal course of cerebral vasculitis induced by neuroborreliosis. | 2010 | 20228487 | |
| prevalence and seasonality of tick-borne pathogens in questing ixodes ricinus ticks from luxembourg. | in europe, ixodid ticks are important arthropod vectors of human and animal pathogens, but comprehensive studies of the prevalence of all relevant pathogens in central europe are scarce. as a result of ecological changes, the incidences of tick-borne infections are expected to increase. in this study, 1,394 nymphal and adult ixodes ricinus ticks sampled monthly during the active season from 33 ecologically distinct collection sites throughout luxembourg were screened for all human tick-borne pat ... | 2010 | 20228110 |
| anti-neural antibody reactivity in patients with a history of lyme borreliosis and persistent symptoms. | some lyme disease patients report debilitating chronic symptoms of pain, fatigue, and cognitive deficits despite recommended courses of antibiotic treatment. the mechanisms responsible for these symptoms, collectively referred to as post-lyme disease syndrome (pls) or chronic lyme disease, remain unclear. we investigated the presence of immune system abnormalities in pls by assessing the levels of antibodies to neural proteins in patients and controls. serum samples from pls patients, post-lyme ... | 2010 | 20227484 |
| atrio-ventricular block as the first presentation of disseminated lyme disease. | a 36year old male patient presented to emergency cardiology department because of fatigability. ecg revealed high grade ii atrio-ventricular block and bradycardia of 31beats/min. an erythema increasing in size to up to 7-8cm in diameter appeared a month earlier and spontaneously resolved within 10days. elisa testing for antibodies against borrelia burgdorferi igm was positive and igg titer was 1:40. intravenous ceftriaxone 2g qod, and 0.5g metronidazole tid lead to regression of grade ii block t ... | 2010 | 20226549 |
| enzymatic synthesis of mycoplasma fermentans specific glycoglycerophospholipid from 1,2-dipalmitoylglycerol. | a gene, mf3, encoding glycosyltransferase in glycoglycerophospholipid (ggpl; ggpl-i and ggpl-iii) biosynthesis in mycoplasma fermentans pg18 was identified by genomic analysis, cloned, modified codon usage, and expressed in escherichia coli. the mf3 gene consists of an open reading frame of 1221 bp encoding 406 amino acids. the mf3 gene product, mf3, has 27% amino acid homology with glycosyltransferase of borrelia burgdorferi but no homology to genes of other mycoplasma species in the genbank da ... | 2010 | 20226374 |
| humoral immune response in dogs naturally infected with borrelia burgdorferi sensu lato and in dogs after immunization with a borrelia vaccine. | lyme arthritis in dogs can be induced under experimental and natural conditions. however, the veterinary relevance of canine borreliosis is still under extensive investigation. the prevalence of symptoms is clearly low, although the risk of tick exposure is high. current research focuses on case definitions, methods for diagnosing clinical disease in dogs, and discrimination between an immune response to a natural infection and an immune response to vaccination. in this experimental study, 23 do ... | 2010 | 20219882 |
| [enzyme immunoassay-based analysis of ospc recombinant proteins from borrelia garinii and borrelia afzelii isolated in west siberia]. | to study the ability of ospc recombinant proteins from borrelia garinii and borrelia afzelii isolated in west siberia to interact with serum antibodies from patients with tick-borne borreliosis (tbb). | 2010 | 20218339 |
| a clinical review of lyme disease in arkansas. | lyme disease is the most common tick-borne disease in the northern hemisphere. since it was first described more than 30 years ago, lyme disease has generated a great deal of controversy. lyme disease is not endemic in arkansas, and testing for borrelia burgdorferi can lead to clinical confusion, unnecessary treatment and excess cost. this article will present a brief review of lyme disease, with an emphasis on what is known regarding lyme disease in arkansas. | 2010 | 20218039 |
| borrelia burgdorferi "sensu lato" in brazil: occurrence confirmed by immunohistochemistry and focus floating microscopy. | in the present study, we report the occurrence of lyme's borreliosis in patients from the brazilian amazon region. borreliosis was investigated by immunohistochemistry and focus floating microscopy for borrelia burgdorferi in skin biopsy samples from 22 patients with both clinical and histopathology evidences compatible with erythema migrans. spirochetes were detected by specific immunohistochemistry and focus floating microscopy for b. burgdorferi in samples from five patients. clinical cure of ... | 2010 | 20211144 |
| [effect of coinfections in ixodidae ticks on transmission of blood microparasites]. | the purpose of this review was to describe and discuss the current spectrum of coinfections in ixodidae ticks and their effects on the transmission of blood microparasites. coinfections with borrelia burgdorferi s. l. and anaplasma phagocytophilum and/or babesia sp. in ticks from poland appear to be common, however, the potential influence on transmission dynamics, the mechanism of genetic variation and the ecology of interactions between pathogens remain poorly understood compared with infectio ... | 2009 | 20209806 |
| lyme borreliosis in dogs and humans in the usa. | borrelia burgdorferi sensu stricto is the only established etiologic agent of lyme borreliosis in dogs and in humans in north america. lyme borreliosis differs in dogs and humans in terms of clinical outcome following infection, diagnostic approaches, prevention strategies and treatment recommendations. nonetheless, serologic evidence of exposure of dogs to b. burgdorferi agrees with the geographical distribution of autochthonous transmission of the agent of lyme borreliosis, and continued monit ... | 2010 | 20207198 |
| borrelia, ehrlichia, and rickettsia spp. in ticks removed from persons, texas, usa. | data regarding the type, frequency, and distribution of tick-borne pathogens and bacterial agents are not widely available for many tick species that parasitize persons in the southern united states. we therefore analyzed the frequency and identity of pathogens and bacterial agents in ticks removed from humans and subsequently submitted to the texas department of state health services, zoonosis control program, from october 1, 2004, through september 30, 2008. the data showed associations of bac ... | 2010 | 20202419 |
| blood meal analysis to identify reservoir hosts for amblyomma americanum ticks. | efforts to identify wildlife reservoirs for tick-borne pathogens are frequently limited by poor understanding of tick-host interactions and potentially transient infectivity of hosts under natural conditions. to identify reservoir hosts for lone star tick (amblyomma americanum)-associated pathogens, we used a novel technology. in field-collected ticks, we used pcr to amplify a portion of the 18s rrna gene in remnant blood meal dna. reverse line blot hybridization with host-specific probes was th ... | 2010 | 20202418 |
| potential for tick-borne bartonelloses. | as worldwide vectors of human infectious diseases, ticks are considered to be second only to mosquitoes. each tick species has preferred environmental conditions and biotopes that determine its geographic distribution, the pathogens it vectors, and the areas that pose risk for tick-borne diseases. researchers have identified an increasing number of bacterial pathogens that are transmitted by ticks, including anaplasma, borrelia, ehrlichia, and rickettsia spp. recent reports involving humans and ... | 2010 | 20202411 |
| two novel salp15-like immunosuppressant genes from salivary glands of ixodes persulcatus schulze tick. | salp15, a 15-kda tick salivary gland protein, is known for several suppressive activities against host immunity and critical functions for the transmission of lyme borrelia in ixodes scapularis and ixodes ricinus, the major vectors found in north america and western europe. salp15 inhibits the activation of cluster of differentiation (cd)4(+)t-cells through the repression of t-cell receptor (tcr)-triggered calcium fluxes and interleukin (il)-2 production. furthermore, salp15 adheres to the spiro ... | 2010 | 20201978 |
| identification of residues within ligand-binding domain 1 (lbd1) of the borrelia burgdorferi ospc protein required for function in the mammalian environment. | borrelia burgdorferi outer surface protein c (ospc) is required for the establishment of infection in mammals. however, its precise function remains controversial. the biologically active form of ospc appears to be a homodimer. alpha helix 1 and 1' of the apposing monomers form a solvent-accessible pocket at the dimeric interface that presents a putative ligand-binding domain (lbd1). here we employ site-directed and allelic-exchange mutagenesis to test the hypothesis that lbd1 is a determinant o ... | 2010 | 20199597 |
| p66 porins are present in both lyme disease and relapsing fever spirochetes: a comparison of the biophysical properties of p66 porins from six borrelia species. | the genus borrelia is the cause of the two human diseases: lyme disease (ld) and relapsing fever (rf). both ld and rf borrelia species are obligate parasites and are dependent on nutrients provided by their hosts. the first step of nutrient uptake across the outer membrane of these gram-negative bacteria is accomplished by water-filled channels, so-called porins. the knowledge of the porin composition in the outer membranes of the different pathogenic borrelia species is limited. only one porin ... | 2010 | 20188698 |
| borreliosis mimicking lupus-like syndrome during infliximab treatment. | tumour necrosis factor (tnf)-alpha antagonists are very effective treatments for immune-mediated inflammatory disorders. adverse events include severe infections and episodes of lupus-like syndrome, multiple sclerosis-like demyelination and other neuropathies. the pathomechanisms of these autoimmune-like syndromes after tnf-alpha blockade are still unknown. we report a patient with psoriasis who developed a lupus-like syndrome during infliximab treatment, which was finally diagnosed as an exagge ... | 2010 | 20184613 |
| inactivation of a putative flagellar motor switch protein flig1 prevents borrelia burgdorferi from swimming in highly viscous media and blocks its infectivity. | the flagellar motor switch complex protein flig plays an essential role in flagella biosynthesis and motility. in most motile bacteria, only one flig homologue is present in the genome. however, several spirochete species have two putative flig genes (referred to as flig1 and flig2) and their roles in flagella assembly and motility remain unknown. in this report, the lyme disease spirochete borrelia burgdorferi was used as a genetic model to investigate the roles of these two flig homologues. it ... | 2010 | 20180908 |
| the role of infection in carotid plaque pathogenesis and stability: the clinical evidence. | chlamydia pneumoniae was the first pathogen linked with carotid atherosclerotic changes and plaque rupture. currently, other common pathogens are also under investigation as potential contributors. | 2010 | 20180770 |
| ecological niche modeling of lyme disease in british columbia, canada. | the purpose of this study was to describe the geographic distribution and model the ecological niche for borrelia burgdorferi (johnson, schmidt, hyde, steigerwaldt & brenner), ixodes pacificus (cooley & kohls), and ixodes angustus (neumann), the bacterium and primary tick vectors for lyme disease, in british columbia (bc), canada. we employed a landscape epidemiology approach using geographic information systems mapping and ecological niche modeling (genetic algorithm for rule-set prediction) to ... | 2010 | 20180315 |
| extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens. | ticks harbor numerous bacterial, protozoal, and viral pathogens that can cause serious infections in humans and domestic animals. active surveillance of the tick vector can provide insight into the frequency and distribution of important pathogens in the environment. nucleic-acid based detection of tick-borne bacterial, protozoan, and viral pathogens requires the extraction of both dna and rna (total nucleic acids) from ticks. traditional methods for nucleic acid extraction are limited to extrac ... | 2010 | 20180313 |
| [aseptic meningitis]. | aseptic meningitis is a benign condition often triggered by a virus or an immunological process. for example herpes virus, borrelia, tuberculosis, a fungus or an autoimmune disease may underlie meningitides presenting prolonged or recurrent symptoms. it is essential to identify the meningitis patients among the diverse group of headache patients and carry out focused investigations and treatment, and in mild cases to avoid complications caused by the investigations. analgesic and antiemetic medi ... | 2009 | 20175326 |
| bba52 facilitates borrelia burgdorferi transmission from feeding ticks to murine hosts. | borrelia burgdorferi, the pathogen of lyme borreliosis, persists in nature through a tick-rodent transmission cycle. a selective assessment of the microbial transcriptome, limited to gene-encoding putative membrane proteins, reveals that bba52 transcription in vivo is strictly confined to the vector-specific portion of the microbial life cycle, with the highest levels of expression noted in feeding ticks and with swift down-regulation noted in mice. bba52 deletion did not affect murine disease a ... | 2010 | 20170377 |
| humoral immune responses in patients with lyme neuroborreliosis. | the aim of this study was to analyze and compare the humoral immune responses in serum and cerebrospinal fluid (csf) for 34 adult patients with clinically evident lyme neuroborreliosis, 27 patients with clinically suspected lyme neuroborreliosis, and 32 patients with tick-borne encephalitis. additionally, we wanted to compare the findings of two methods for the detection of intrathecally synthesized borrelial antibodies: the ideia lyme neuroborreliosis test using flagellar antigen and an approac ... | 2010 | 20164248 |
| a novel surface antigen of relapsing fever spirochetes can discriminate between relapsing fever and lyme borreliosis. | in a previous immunoproteome analysis of borrelia hermsii, candidate antigens that bound igm antibodies from mice and patients infected with relapsing fever spirochetes were identified. one candidate that was identified is a hypothetical protein with a molecular mass of 57 kda that we have designated borrelia immunogenic protein a (bipa). this protein was further investigated as a potential diagnostic antigen for b. hermsii given that it is absent from the borrelia burgdorferi genome. the bipa l ... | 2010 | 20147497 |
| comment on 'membranous glomerulonephritis secondary to borrelia burgdorferi infection presenting as nephrotic syndrome'. | 2010 | 20147284 | |
| activation of innate host defense mechanisms by borrelia. | borrelia is the causative agent of lyme disease, a widespread disease with important health consequences. immune-mediated mechanisms are believed to play a major role in both host defense and in late complications of lyme disease. recognition of borrelia and the initial activation of the innate immune system are important for host defense, as well as modulation of adaptive responses. several classes of pattern recognition receptors (prrs) have been suggested to be involved in the recognition of ... | 2010 | 20146985 |
| identification and functional characterisation of complement regulator acquiring surface protein-1 of serum resistant borrelia garinii ospa serotype 4. | b. burgdorferi sensu lato (sl) is the etiological agent of lyme borreliosis in humans. spirochetes have adapted themselves to the human immune system in many distinct ways. one important immune escape mechanism for evading complement activation is the binding of complement regulators factor h (cfh) or factor h-like protein1 (fhl-1) to complement regulator-acquiring surface proteins (crasps). | 2010 | 20146822 |
| canine and feline vector-borne diseases in italy: current situation and perspectives. | in italy, dogs and cats are at risk of becoming infected by different vector-borne pathogens, including protozoa, bacteria, and helminths. ticks, fleas, phlebotomine sand flies, and mosquitoes are recognized vectors of pathogens affecting cats and dogs, some of which (e.g., anaplasma phagocytophilum, borrelia burgdorferi, dipylidium caninum, leishmania infantum, dirofilaria immitis, and dirofilaria repens) are of zoonotic concern. recent studies have highlighted the potential of fleas as vectors ... | 2010 | 20145730 |
| concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis. | about 500 million cases of malaria occur annually. however, a substantial number of patients who actually have relapsing fever (rf) borrelia infection can be misdiagnosed with malaria due to similar manifestations and geographic distributions of the two diseases. more alarmingly, a high prevalence of concomitant infections with malaria and rf borrelia has been reported. therefore, we used a mouse model to study the effects of such mixed infection. we observed a 21-fold increase in spirochete tit ... | 2010 | 20145098 |
| endometriosis-associated lyme disease. | the aim of this study is to report three cases of patients with endometriosis and infertility, and associated with lyme disease. the medical files of 405 women with endometriosis and 200 without endometriosis were studied retrospectively. we report 3 cases with endometriosis and lyme disease. of 405 patients with endometriosis treated in our study over a 6-year period, 3(0.8%) had lyme disease. all cases presented with typical erythema migraines, fever and fatigue. the serological findings were ... | 2010 | 20143981 |
| chemoenzymatic synthesis of a glycolipid library and elucidation of the antigenic epitope for construction of a vaccine against lyme disease. | lyme disease (ld) is the most common tick-borne disease in europe, north america, and asia. the etiologic agents of ld are spirochetes of the group borrelia burgdorferi sensu lato, which possess a lipid content of 25-30% of the dry weight. the major glycolipid cholesteryl 6-o-acyl-beta-d-galactopyranoside (acgal), present in b. burgdorferi sensu stricto, b. afzelii, and b. garinii, is a specific and highly prevalent antigen frequently recognized by antibodies in late-stage ld. here we report a c ... | 2010 | 20143357 |
| salivary gland extract from engorged ixodes ricinus (acari: ixodidae) stimulates in vitro growth of borrelia burgdorferi sensu lato. | in vitro effect of salivary gland extract from fed ixodes ricinus, the competent vector of lyme borreliosis in europe, on the growth of borrelia burgdorferi sensu lato (b. garinii, b. afzelii and b. burgdorferi sensu stricto) was examined in bsk-h medium. motility rate, concentration of motile spirochetes and their morphology were estimated at intervals of 0, 2, 4, 6 and 8 days using darkfield microscopy. salivary gland extract derived from i. ricinus stimulated markedly the growth of three geno ... | 2010 | 20143350 |
| a bacterial elongation factor g homologue exclusively functions in ribosome recycling in the spirochaete borrelia burgdorferi. | translation elongation factor g (ef-g) in bacteria plays two distinct roles in different phases of the translation system. ef-g catalyses the translocation of trnas on the ribosome in the elongation step, as well as the dissociation of the post-termination state ribosome into two subunits in the recycling step. in contrast to this conventional view, it has very recently been demonstrated that the dual functions of bacterial ef-g are distributed over two different ef-g paralogues in human mitocho ... | 2010 | 20132446 |
| lyme borreliosis: clinical case definitions for diagnosis and management in europe. | lyme borreliosis, caused by spirochaetes of the borrelia burgdorferi genospecies complex, is the most commonly reported tick-borne infection in europe and north america. the non-specific nature of many of its clinical manifestations presents a diagnostic challenge and concise case definitions are essential for its satisfactory management. lyme borreliosis is very similar in europe and north america but the greater variety of genospecies in europe leads to some important differences in clinical p ... | 2011 | 20132258 |
| methods of identifying membrane proteins in spirochetes. | bacterial membrane proteins serve vital functions such as nutrient acquisition, sensation of the environment, and in gene regulation, secretion, and attachment. proteins on the cell surface are instrumental in host-pathogen interactions and many serve as immunogens that confer protection as targets for neutralizing antibodies. integral membrane and lipidated proteins possess hydrophobic domains or lipid anchors that interact with the lipid bilayer of cellular membranes, allowing the investigator ... | 2010 | 20131222 |
| [lyme disease. the carrier tick has settled in quebec]. | 2009 | 20120304 | |
| lyme disease presenting as subacute transverse myelitis. | lyme disease (borreliosis) is a systemic illness resulting from infection with the spirochete borrelia burgdorferi. it is transmitted to humans by the bites of infected ticks belonging to several species of the genus ixodes. after the bacteria enter the body via the dermis, most patients develop the early, localised form of lyme disease, which is characterised by erythema migrans and influenza-like symptoms. this disease may also affect the heart, nervous system and joints. the neurological find ... | 2009 | 20120216 |
| analysis of the dbpba upstream regulatory region controlled by rpos in borrelia burgdorferi. | decorin-binding proteins b and a (dbpb and dbpa) are thought to play important roles in borrelia burgdorferi pathogenesis by serving as adhesins for the extracellular matrix. it has been established that the expression of dbpba is governed by the rrp2-rpon-rpos regulatory pathway. however, the precise mechanism underlying the control of dbpba expression has been unclear. in particular, it has been unknown whether rpos influences dbpba expression directly or indirectly (through an additional regu ... | 2010 | 20118265 |
| endemic tickborne infectious diseases in louisiana and the gulf south. | most emerging infectious diseases today, such as west nile virus and sudden acute respiratory distress syndrome (sars), arise from zoonotic reservoirs and many are transmitted by arthropod vectors. ticks are among the most competent and versatile arthropod vectors of infectious diseases because ticks of all ages and both sexes remain infectious for generations without having to reacquire infections from reservoir hosts. today, ticks transmit the most common arthropod-borne infectious disease in ... | 2009 | 20108827 |
| butterfly rash in a young boy: a quiz. | 2010 | 20107747 | |
| the chitobiose transporter, chbc, is required for chitin utilization in borrelia burgdorferi. | the bacterium borrelia burgdorferi, the causative agent of lyme disease, is a limited-genome organism that must obtain many of its biochemical building blocks, including n-acetylglucosamine (glcnac), from its tick or vertebrate host. glcnac can be imported into the cell as a monomer or dimer (chitobiose), and the annotation for several b. burgdorferi genes suggests that this organism may be able to degrade and utilize chitin, a polymer of glcnac. we investigated the ability of b. burgdorferi to ... | 2010 | 20102636 |
| evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for ehrlichia, anaplasma, spotted fever group rickettsia, bartonella, and borrelia species in canine neurological diseases (109 cases). | vector-transmitted microorganisms in the genera ehrlichia, anaplasma, rickettsia, bartonella, and borrelia are commonly suspected in dogs with meningoencephalomyelitis (mem), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (csf) of dogs with mem is unknown. | 2010 | 20102497 |
| update on tick-borne bacterial diseases in europe. | in recent years, the prevalence of tick-borne bacterial diseases has significantly increased in european countries. the emergence and reemergence of these illnesses are attributed to changes in the environment and human behavior. several diseases are caused by bacteria initially isolated from ticks and subsequently considered pathogenic. it is necessary to consider the bacteria found in arthropods capable of biting humans as potential human pathogens. here we review the clinical and epidemiologi ... | 2009 | 20092057 |
| [intermediate uveitis as the "first symptom" of multiple sclerosis]. | 2010 | 20091454 | |
| coinfection of western gray squirrel (sciurus griseus) and other sciurid rodents with borrelia burgdorferi sensu stricto and anaplasma phagocytophilum in california. | overlapping geographic distributions of tick-borne disease agents utilizing the same tick vectors are common, and coinfection of humans, domestic animals, wildlife, and ticks with both borrelia burgdorferi and anaplasma phagocytophilum has been frequently reported. this study was undertaken in order to evaluate the prevalence of both b. burgdorferi sensu stricto (hereinafter referred to as b. burgdorferi) and a. phagocytophilum in several species of sciurid rodents from northern california, usa. ... | 2010 | 20090047 |
| seroprevalence of borrelia burgdorferi sensu lato and anaplasma phagocytophilum in danish horses. | borrelia burgdorferi sensu lato and anaplasma phagocytophilum are able to infect horses. however, the extend to which danish horses are infected and seroconvert due to these two bacteria is unknown. the aim of the present study was to evaluate the seroprevalence of b. burgdorferi sensu lato and a. phagocytophilum in danish horses. | 2010 | 20082693 |
| identical phosphatase mechanisms achieved through distinct modes of binding phosphoprotein substrate. | two-component signal transduction systems are widespread in prokaryotes and control numerous cellular processes. extensive investigation of sensor kinase and response regulator proteins from many two-component systems has established conserved sequence, structural, and mechanistic features within each family. in contrast, the phosphatases which catalyze hydrolysis of the response regulator phosphoryl group to terminate signal transduction are poorly understood. here we present structural and fun ... | 2010 | 20080618 |
| lipid binding orientation within cd1d affects recognition of borrelia burgorferi antigens by nkt cells. | invariant natural killer t cells (inkt cells) respond to cd1d-presented glycolipids from borrelia burgdorferi, the causative agent of lyme disease. although mouse and human inkt cells respond to different antigens based on subtle differences in their fatty acids, the mechanism by which fatty acid structure determines antigenic potency is not well understood. here we show that the mouse and human cd1d present glycolipids having different fatty acids, based in part upon a difference at a single am ... | 2010 | 20080535 |
| [recurrent nerve palsy due to lyme disease: report of two cases]. | neuroborreliosis can be a difficult diagnosis which requires epidemiologic, clinical and biologic arguments. | 2010 | 20079561 |
| a new view on lyme disease: rodents hold the key to annual risk. | 2006 | 20076583 | |
| antibiotic treatment duration and long-term outcomes of patients with early lyme disease from a lyme disease-hyperendemic area. | the length of antibiotic therapy and long-term outcomes in patients with early lyme disease are incompletely described. we report the long-term clinical outcomes of patients with early localized and early disseminated lyme disease based on the duration of antibiotic therapy prescribed. | 2010 | 20070237 |
| a commentary on the treatment of early lyme disease. | 2010 | 20070236 | |
| cysteine-free proteins in the immunobiology of arthropod-borne diseases. | one approach to identify epitopes that could be used in the design of vaccines to control several arthropod-borne diseases simultaneously is to look for common structural features in the secretome of the pathogens that cause them. using a novel bioinformatics technique, cysteine-abundance and distribution analysis, we found that many different proteins secreted by several arthropod-borne pathogens, including plasmodium falciparum, borrelia burgdorferi, and eight species of proteobacteria, are de ... | 2010 | 20069123 |
| treatment of lyme borreliosis. | borrelia burgdorferi sensu lato is the causative agent of lyme borreliosis in humans. this inflammatory disease can affect the skin, the peripheral and central nervous system, the musculoskeletal and cardiovascular system and rarely the eyes. early stages are directly associated with viable bacteria at the site of inflammation. the pathogen-host interaction is complex and has been elucidated only in part. b. burgdorferi is highly susceptible to antibiotic treatment and the majority of patients p ... | 2009 | 20067594 |
| prevalence of bartonella henselae and borrelia burgdorferi sensu lato dna in ixodes ricinus ticks in europe. | bartonella spp. can cause persistent bloodstream infections in humans and animals. to determine whether bartonella henselae is present in questing ixodes ricinus ticks, we analyzed the prevalence of b. henselae dna among tick stages compared to the prevalence of dna from borrelia burgdorferi sensu lato, the pathogen most frequently transmitted by ticks. b. henselae dna was present with a prevalence of up to approximately 40% in tick populations sampled in four european sites (eberdingen, germany ... | 2010 | 20061459 |
| [cross-reaction evaluation of pcr-anaplasma platys positive dogs tested to anaplasma phagocytophilum antibodies by commercial elisa]. | anaplasma platys, agent of canine cyclic thrombocytopenia parasites exclusively dogs platelets. its probable vector is the tick rhipicephalus sanguineus. among the existent diagnostic methods, the most used include: morulae identification in blood smears; antibody detection by indirect immunofluorescence; or dna amplification by polymerase chain reaction (pcr). recently a new commercial elisa (idexx®), capable of detecting a. phagocytophilum antibodies, has been developed. according to the manuf ... | 2008 | 20059806 |
| presence of borrelia burgdorferi in endomyocardial biopsies in patients with new-onset unexplained dilated cardiomyopathy. | dilated cardiomyopathy (dcm) represents the third most common cause of heart failure and the most frequent cause of heart transplantation. infectious, mostly viral, and autoimmune mechanisms, together with genetic abnormalities, have been reported as three major causes of dcm. we hypothesized that lyme disease (ld), caused by spirochete borrelia burgdorferi (bb), might be an important cause of new-onset unexplained dcm in patients living in a highly endemic area for ld such as the czech republic ... | 2010 | 20052487 |
| [the heterogeneity of the ospa gene of borrelia garinii and borrelia afzelii in western siberia and mongolia]. | 48 full-length borrelia garinii and borrelia afzelii from west siberia and mongolia ospa gene nucleotide sequences was determined. four groups of borrelia garinii were revealed using the analysis of nucleotide sequences. the most variable ospa gene region was demonstrated to be included in region where the antigenic determinants of protein were encoded. high homology level was shown for nucleotide sequences corresponding to isolates of borrelia afzelii. | 2009 | 20050161 |
| trypanosoma cf. varani in an imported ball python (python reginus) from ghana. | peripheral blood from a ball python (python reginus) imported from ghana was cultured in barbour-stoenner-kelly (bsk) medium for borrelia spp. isolation, resulting in the prominent appearance of free, and clusters of, trypanosomes in a variety of morphological forms. the molecular phylogenetic characterization of these cultured trypanosomes, using the small subunit rdna, indicated that this python was infected with a species closely related to trypanosoma varani wenyon, 1908, originally describe ... | 2009 | 20050011 |
| the occurrence of toxoplasma gondii and borrelia burgdorferi sensu lato in ixodes ricinus ticks from east poland with the use of pcr. | 715 ixodes ricinus ticks from 4 regions of lublin macroregion (piotrowice, polesie national park, dabrowa and leczynsko-wlodawskie lakeland) were examined for the presence of borrelia burgdorferi sensu lato and toxoplasma gondi, using polymerase chain reaction (pcr) and nested-pcr methods. the clonal type of samples tested positive for t. gondii was identified by amplification and fragmenting with restrictions enzymes (rflp-restriction fragment length polymorphism). positive results were confirm ... | 2009 | 20047269 |
| occurrence of igg antibodies to anaplasma phagocytophilum in humans suspected of lyme borreliosis in eastern slovakia. | human granulocytic anaplasmosis (hga) and lyme borreliosis (lb) are tick-borne and emerging infectious diseases caused by the anaplasma phagocytophilum and borrelia burgdorferi species. in europe, including slovakia, the principal vector of both pathogens is the common tick - ixodes ricinus, in which double infections with these pathogens have been reported. the aim of our study was evidence of igg antibodies against a. phagocytophilum in blood sera of humans with suspects lb from several clinic ... | 2009 | 20047263 |
| what is the percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae? | in this study, larvae (1,179 ex.) of mosquito genera culex were examined for the presence of spirochaetes by dark field microscopy (dfm) at the locality of blansko (czech republic) in of 2004-2008. dfm spirochaete positive samples (25.4 %) were investigated by nested pcr; only 4 samples were positive for the presence of borrelia burgdorferi sensu lato, which is 0.3 % of the total examined samples. we can conclude that only a low percentage of pathogenic borreliae are presented in mosquito larvae ... | 2009 | 20047261 |
| seroepidemiological study of lyme borreliosis among forestry workers in southern poland. | forestry workers are a professional group particularly exposed to tick-borne infections; however, continuous monitoring of anti-borrelia burgdorferi antibodies in this group enables faster diagnosis and more effective treatment for borreliosis. a group of 1,155 forestry workers from six forest inspectorates in southern poland were examined with the immunoenzymatic method (elisa test). the general level of anti-borrelia burgdorferi antibodies was 12.8 % in igm class, and 25 % in igg class. marked ... | 2009 | 20047259 |
| occurrence of cases of borreliosis certified as an occupational disease in the province of wielkopolska (poland). | the aim of the study was to analyze the occurrence of cases of borreliosis to classify as an occupational disease in the province [voivodeship] of wielkopolska in the years 2000-2007. the data as a basis for this analysis originated from the database on the occupational disease certification created by the public health service in poznan. documentation from the years 2000-2007 reviewed. the maximum number of the cases of borreliosis in the territory of wielkopolska certified as the occupational ... | 2009 | 20047253 |
| the borrelial fibronectin-binding protein reva is an early antigen of human lyme disease. | previous studies using small numbers of serum samples from human patients and experimentally infected animals identified the frequent presence of antibodies recognizing reva, a borrelial fibronectin-binding outer surface protein. we now demonstrate that most examined lyme disease spirochetes from north america and europe contain genes encoding reva proteins, some with extensive regions of conservation and others with moderate diversity. line blot analyses using recombinant reva from two diverse ... | 2010 | 20032216 |