Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| roles for phagocytic cells and complement in controlling relapsing fever infection. | relapsing fever spirochetes, such as borrelia hermsii, proliferate to high levels in their hosts' bloodstream until production of igm against borrelial surface proteins promotes bacterial clearance. the mechanisms by which b. hermsii survives in host blood, as well as the immune mediators that control this infection, remain largely unknown. it has been hypothesized that b. hermsii is naturally resistant to killing by the alternative pathway of complement activation as a result of its ability to ... | 2009 | 19458267 |
| antibiotic responsive demyelinating neuropathy related to lyme disease. | 2009 | 19451535 | |
| cspa-mediated binding of human factor h inhibits complement deposition and confers serum resistance in borrelia burgdorferi. | borrelia burgdorferi has developed efficient mechanisms for evading the innate immune response during mammalian infection and has been shown to be resistant to the complement-mediated bactericidal activity of human serum. it is well recognized that b. burgdorferi expresses multiple lipoproteins on its surface that bind the human complement inhibitors factor h and factor h-like protein 1 (fh/fhl-1). the binding of fh/fhl-1 on the surface of b. burgdorferi is thought to enhance its ability to evad ... | 2009 | 19451251 |
| [epidemiology of lyme borreliosis and neuroborreliosis in france]. | lyme borreliosis (lb) is a systemic disease called neuroborreliosis (nb) when neurological symptoms are pre-eminent. lb is a zoonosis caused by borrelia bacteria transmitted by ixodes tick-bite. because of the absence of a national registry, epidemiology of lb in france is not well known. moreover, diagnosis of nb may be difficult because of the various clinical forms. acute meningoradiculitis is the most common presentation, but pauci-symptomatic meningitis, encephalitis, myelitis, polyneuropat ... | 2009 | 19447458 |
| [facial palsy in a 7-year-old german tourist]. | 2009 | 19446508 | |
| identification of conserved antigens for early serodiagnosis of relapsing fever borrelia. | borrelia hermsii is a blood-borne pathogen transmitted by the argasid tick ornithodoros hermsi. since spirochaete clearance in mice is associated with an igm-mediated response, an immunoproteomic analysis was used to identify proteins reactive with igm. we report that igm from both mice and human patients infected with b. hermsii not only reacted with the previously identified variable membrane proteins but also identified candidate antigens including heat-shock proteins, an adhesin protein, abc ... | 2009 | 19443544 |
| [icelandic boy with lyme-disease. a case report and discussion.] | lyme-disease is an infection involving many organ systems and is caused by the spirochete borrelia burgdorferi. lyme-disease is the most prevalent vector-born disease in the united states and also occurs in parts of europe and asia. however, to the best of our knowledge, lyme-disease has not been described in iceland before. in this article we report a 14-year-old, icelandic boy with arthritis due to lyme-disease. lyme-disease was described as a separate entity in 1976. the identification of a n ... | 1999 | 19439803 |
| intact flagellar motor of borrelia burgdorferi revealed by cryo-electron tomography: evidence for stator ring curvature and rotor/c-ring assembly flexion. | the bacterial flagellar motor is a remarkable nanomachine that provides motility through flagellar rotation. prior structural studies have revealed the stunning complexity of the purified rotor and c-ring assemblies from flagellar motors. in this study, we used high-throughput cryo-electron tomography and image analysis of intact borrelia burgdorferi to produce a three-dimensional (3-d) model of the in situ flagellar motor without imposing rotational symmetry. structural details of b. burgdorfer ... | 2009 | 19429612 |
| follow-up of bernese mountain dogs and other dogs with serologically diagnosed borrelia burgdorferi infection: what happens to seropositive animals? | data on the long-term outcome of b. burgdorferi infections in adult dogs are sparse. the aim of the present study was to investigate whether bernese mountain dogs with serological evidence of natural b. burgdorferi infection more often develop signs such as lameness, azotemia or proteinuria during a follow-up period of 2.5 to 3.0 years. seropositive bernese mountain dogs were compared to seronegative bernese mountain dogs and to seropositive and seronegative control dogs of other breeds. dogs in ... | 2009 | 19426490 |
| cerebrospinal fluid pcr and antibody concentrations against anaplasma phagocytophilum and borrelia burgdorferi sensu lato in dogs with neurological signs. | the tick-borne bacteria borrelia burgdorferi sensu lato (sl) and anaplasma phagocytophilum have been suspected to cause neurological signs in dogs. diagnosis often has been made based on positive antibody titers in serum of dogs with neurological signs, but a high seroprevalence in dogs in at-risk populations makes diagnosis difficult. | 2009 | 19422467 |
| concomitant human granulocytic anaplasmosis and lyme neuroborreliosis. | 2009 | 19416290 | |
| ticks and tick-borne pathogens and putative symbionts of black bears (ursus americanus floridanus) from georgia and florida. | ticks were collected from 38 black bears (ursus americanus floridanus) from northwestern florida (n = 18) from 2003 to 2005 and southern georgia (n = 20) in 2006. five species (amblyomma americanum, a. maculatum, dermacentor variabilis, ixodes scapularis, and i. affinis) were collected from florida bears, and 4 species (a. americanum, a. maculatum, d. variabilis, i. scapularis) were collected from bears in georgia. ixodes scapularis was the most frequently collected tick, followed by d. variabil ... | 2009 | 19413369 |
| levels of svcam-1 and sicam-1 in patients with lyme disease. | lyme disease is a multi-organ animal-borne disease caused by the spirochete borrelia burgdorferi (bb). | 2009 | 19413177 |
| the epidemiology of tick-borne relapsing fever in iran during 1997-2006. | tick-borne relapsing fever is an acute febrile and endemic disease in iran. for many reasons, the incidence of disease is on decrease, however tick-borne relapsing fever is still a health issue in the rural areas for travelers. this study was carried out during 1997-2006 to investigate the tick-borne relapsing fever in iran. | 2009 | 19411042 |
| a novel fibronectin binding motif in mscramms targets f3 modules. | bbk32 is a surface expressed lipoprotein and fibronectin (fn)-binding microbial surface component recognizing adhesive matrix molecule (mscramm) of borrelia burgdorferi, the causative agent of lyme disease. previous studies from our group showed that bbk32 is a virulence factor in experimental lyme disease and located the fn-binding region to residues 21-205 of the lipoprotein. | 2009 | 19404402 |
| [cutaneous marginal zone lymphoma (salt) and infection with borrelia burgdorferi]. | a relationship between borrelia burgdorferi and the development of cutaneous b-cell lymphoma (cbcl) has been long discussed. b. burgdorferi dna has been detected in patients with cbcl and a response of cbcl to antibiotics has been observed. in our patient with a borrelia infection, a marginal zone lymphoma (salt) regressed after ceftriaxone therapy. this further case of a combined appearance of cbcl and b. burgdorferi underlines a possible relationship as an example of an infectious trigger in t ... | 2010 | 19399378 |
| borrelia burgdorferi reva antigen binds host fibronectin. | borrelia burgdorferi, the lyme disease-causing spirochete, can persistently infect its vertebrate hosts for years. b. burgdorferi is often found associated with host connective tissue, where it interacts with components of the extracellular matrix, including fibronectin. some years ago, a borrelial surface protein, named bbk32, was identified as a fibronectin-binding protein. however, b. burgdorferi bbk32 mutants are still able to bind fibronectin, indicating that the spirochete possesses additi ... | 2009 | 19398540 |
| ospc-independent infection and dissemination by host-adapted borrelia burgdorferi. | borrelia burgdorferi ospc is required for the spirochete to establish infection in a mammal by tick transmission or needle inoculation. after a brief essential period, the protein no longer is required and the gene can be shut off. using a system in which spirochetes contain only an unstable wild-type copy of the ospc gene, we can obtain mice persistently infected with bacteria lacking ospc. we implanted pieces of infected mouse skin subcutaneously in naïve mice, using donors carrying wild-type ... | 2009 | 19398538 |
| [lyme borreliosis]. | lyme borreliosis is a multi-system infectious disease that primarily affects the skin, nervous system, heart, and joints. it is caused by the tick-borne spirochete borrelia burgdorferi sensu lato. diagnosis is made on the basis of clinical symptoms and supported by a positive serology. antibiotic therapy should be started immediately after the diagnosis has been established and is administered according to stage and symptoms of the disease. doxycycline, amoxicillin, and ceftriaxone are the antib ... | 2009 | 19387665 |
| serological and molecular detection of borrelia burgdorferi sensu lato and anaplasmataceae in rodents. | the presence of anaplasma spp. and borrelia burgdorferi sensu lato in rodents from eastern slovakia were followed by serological and molecular methods. the seroprevalence for borrelia was detected in 16.6 %, for anaplasmataceae (apt) in 13.2 % and co-occurrence of borrelia and apt in 7.5 %. out of 110 ear biopsies of rodents, 5 were b. afzelii-positive. five biopsies tested positive with the ehr521-ehr747 primers amplifying all the members of the family apt. a. phagocytophilum was detected in 1. ... | 2009 | 19381473 |
| microbial communities and interactions in the lone star tick, amblyomma americanum. | to quantify microbial composition and interactions, we identified prokaryotic communities in the lone star tick (amblyomma americanum) based on 16s rrna gene sequences and direct probing. the lone star tick is the vector of emerging diseases and host to additional symbionts of unknown activity, and is representative of other blood-sucking arthropods. we evaluated the potential for vertical (transovarial) transmission by molecular analysis of microbial symbionts from egg and larval clutches. dire ... | 2008 | 19378409 |
| assessment of methylthioadenosine/s-adenosylhomocysteine nucleosidases of borrelia burgdorferi as targets for novel antimicrobials using a novel high-throughput method. | lyme disease is the most prevalent tick-borne disease in the usa with the highest number of cases (27 444 patients) reported by cdc in the year 2007, representing an unprecedented 37% increase from the previous year. the haematogenous spread of borrelia burgdorferi to various tissues results in multisystemic disease affecting the heart, joints, skin, musculoskeletal and nervous system of the patients. | 2009 | 19376840 |
| the c6 lyme antibody test has low sensitivity for antibody detection in cerebrospinal fluid. | our aim was to evaluate the performance of the commercial immunetics c6 lyme elisa assay as a screening assay for anti-borrelia burgdorferi antibodies in cerebrospinal fluid (csf). sensitivity of c6 elisa was determined in 28 consecutive patients who were diagnosed with neuroborreliosis and had evidence for intrathecal antibody synthesis on immunoblot analysis. the presence of additional bands in csf or of bands with a stronger intensity in csf compared with serum was considered evidence of intr ... | 2009 | 19376674 |
| [familial predisposition and microbial etiology in dilated cardiomyopathy]. | cardiomyopathies are an important and diverse group of heart muscle diseases in which the heart muscle itself is structurally or functionally abnormal and in which coronary artery disease, hypertension, valvular and congenital heart disease are absent or do not sufficiently explain the observed myocardial abnormality. this often results in severe heart failure accompanied by arrhythmias and/or sudden death. clinical and morphological diversity of cardiomyopathies can reflect the broad spectrum o ... | 2009 | 19370326 |
| a yeast-endonuclease-generated dna break induces antigenic switching in trypanosoma brucei. | trypanosoma brucei is the causative agent of african sleeping sickness in humans and one of the causes of nagana in cattle. this protozoan parasite evades the host immune system by antigenic variation, a periodic switching of its variant surface glycoprotein (vsg) coat. vsg switching is spontaneous and occurs at a rate of about 10(-2)-10(-3) per population doubling in recent isolates from nature, but at a markedly reduced rate (10(-5)-10(-6)) in laboratory-adapted strains. vsg switching is thoug ... | 2009 | 19369939 |
| reviewing molecular adaptations of lyme borreliosis spirochetes in the context of reproductive fitness in natural transmission cycles. | lyme borreliosis (lb) is caused by a group of pathogenic spirochetes - most often borrelia burgdorferi, b. afzelii, and b. garinii - that are vectored by hard ticks in the ixodes ricinus-persulcatus complex, which feed on a variety of mammals, birds, and lizards. although lb is one of the best-studied vector-borne zoonoses, the annual incidence in north america and europe leads other vector-borne diseases and continues to increase. what factors make the lb system so successful, and how can resea ... | 2009 | 19368764 |
| nervous system lyme disease: diagnosis and treatment. | lyme disease, the multisystem infectious disease caused by the tickborne spirochete borrelia burgdorferi, frequently affects the peripheral and central nervous systems. the earliest indication of lyme disease infection is usually erythema migrans. this large, typically macular erythema, often with a target-like pattern of concentric pale and red circles, gradually enlarges day by day, potentially reaching many centimeters in diameter. in a significant proportion of infected individuals, an acute ... | 2009 | 19367218 |
| what are the indications for lumbar puncture in patients with lyme disease? | lyme neuroborreliosis (lnb) is a tick-borne disease of the nervous system, caused by the spirochete borrelia burgdorferi. having entered the host at the site of the tick bite, the spirochetes can initially cause a local inflammatory reaction, called erythema migrans. if left untreated, the borrelia can disseminate in the second stage of the disease and invade the central nervous system, causing lnb. the diagnosis of lnb is based on a compatible clinical picture (meningitis, cranial neuritis or r ... | 2009 | 19367105 |
| is serological follow-up useful for patients with cutaneous lyme borreliosis? | serologic follow-up examinations are frequently performed in patients with erythema migrans, borrelial lymphocytoma, and acrodermatitis chronica atrophicans (the 3 dermatoborrelioses) to evaluate treatment efficacy. there is, however, substantial proof in the literature that antibody titer development after therapy is unpredictable and variable, and moreover it is largely uncorrelated with the clinical course and mode of antibiotic treatment. for example, persistent positive igg and/ or igm anti ... | 2009 | 19367102 |
| what should one do in case of a tick bite? | ixodes ricinus is the commonest tick species in europe, and transmits lyme borreliosis, tick-borne encephalitis, ehrlichiosis, tularemia, rickettsiosis, and babesiosis. the risk of borrelia burgdorferi infection increases with the time of tick engorgement, but not every infection necessarily causes erythema migrans or lyme borreliosis. therefore, the finding of b. burgdorferi dna in a tick does not prove that the patient will subsequently develop lyme borreliosis. ticks should be removed as earl ... | 2009 | 19367100 |
| life cycle of borrelia burgdorferi sensu lato and transmission to humans. | lyme borreliosis is a zoonosis: its causative agent, borrelia burgdorferi sensu lato, circulates between ixodes ricinus ticks and a large variety of vertebrates. i. ricinus has a wide geographical distribution throughout europe within the latitudes of 65 degrees and 39 degrees and from portugal into russia. enzootic cycles in europe involve at least 7 borrelia species. apparently, associations exist in nature between borrelia species and hosts. b. afzelii and b. burgdorferi sensu stricto are ass ... | 2009 | 19367095 |
| borrelia burgdorferi sensu lato diversity and its influence on pathogenicity in humans. | among the spirochaetes, the borrelia burgdorferi sensu lato complex is responsible for lyme borreliosis. this complex comprises more than 13 borrelia species. four of them are clearly pathogenic for humans: b. burgdorferi sensu stricto, b. afzelii, b. garinii and b. spielmanii. they can generate erythema migrans, an initial skin lesion, and can then spread deeply into the host to invade distant tissues, especially the nervous system, the joints or the skin. in humans, borrelia pathogenicityseems ... | 2009 | 19367094 |
| matrix metalloproteinase 9 plays a key role in lyme arthritis but not in dissemination of borrelia burgdorferi. | borrelia burgdorferi, the causative agent of lyme arthritis, does not produce any exported proteases capable of degrading extracellular matrix despite the fact that it is able to disseminate from a skin insertion site to infect multiple organs. prior studies have shown that b. burgdorferi induces the host protease, matrix metalloproteinase 9 (mmp-9), and suggested that the induction of mmp-9 may allow the organism to disseminate and produce local tissue destruction. we examined the role of mmp-9 ... | 2009 | 19364840 |
| detection of the dna of borrelia afzelii, anaplasma phagocytophilum and babesia canis in blood samples from dogs in warsaw. | each month, from march 2003 to february 2004, 34 blood samples from dogs were randomly selected from the blood samples delivered to two veterinary laboratories in warsaw and tested for the dna of borrelia burgdorferi sensu lato, anaplasma phagocytophilum, babesia canis and hepatozoon canis. borrelia dna was detected in seven of the 408 dogs, a phagocytophilum dna was found in two, and b canis dna was found in 48 (11.8 per cent). the dna of h canis was not found in any of the blood samples. seque ... | 2009 | 19363228 |
| [lyme borreliosis--experience of the last 25 years in hungary]. | we recognized the first hungarian lyme patients just 25 years ago, in 1984. it was exactly 20 years ago, when we opened the lyme disease outpatient service at the central (lászló) hospital for infectious diseases. 15 years ago we established the financially independent center for tick-borne diseases. the milestones of this work at the center for tick-borne diseases are the description of a new tick-borne rickettsial illness (tick-borne lymphadenopathy), development of a lyme immunoblot kit and a ... | 2009 | 19362925 |
| the expanding spectrum of cutaneous borreliosis. | the known spectrum of skin manifestations in cutaneous lyme disease is continuously expanding and can not be regarded as completed. besides the classical manifestations of cutaneous borreliosis like erythema (chronicum) migrans, borrelial lymphocytoma and acrodermatitis chronica atrophicans evidence is growing that at least in part also other skin manifestations, especially morphea, lichen sclerosus and cases of cutaneous b-cell lymphoma are causally related to infections with borrelia. also gra ... | 2009 | 19357623 |
| lyme neuroborreliosis: manifestations of a rapidly emerging zoonosis. | lyme disease has a worldwide distribution and is the most common vector-borne disease in the united states. incidence, clinical manifestations, and presentations vary by geography, season, and recreational habits. lyme neuroborreliosis (lnb) is neurologic involvement secondary to systemic infection by the spirochete borrelia burgdorferi in the united states and by borrelia garinii or borrelia afzelii species in europe. enhanced awareness of the clinical presentation of lyme disease allows inclus ... | 2009 | 19346313 |
| anaplasma phagocytophilum and borrelia burgdorferi in rabbits from southeastern china. | a total of 54 wild rabbits captured from southeastern china were examined for anaplasma phagocytophilum and borrelia burgdorferi sensu lato by polymerase chain reaction (pcr) assays. one and three samples were positive for a. phagocytophilum and b. burgdorferi, respectively. sequence analyses of pcr products identified a variant of a. phagocytophilum and a b. garinii genotype. this is the first detection of the two tick-borne agents in chinese rabbits, the role of which in the maintenance of the ... | 2009 | 19345508 |
| strong igg antibody responses to borrelia burgdorferi glycolipids in patients with lyme arthritis, a late manifestation of the infection. | in this study, the membrane lipids of b. burgdorferi were separated into 16 fractions; the components in each fraction were identified, and the immunogenicity of each fraction was determined by elisa using sera from lyme disease patients. only the 2 glycolipids, acylated cholesteryl galactoside (acg, bbgl-i) and monogalactosyl diacylglycerol (mgald, bbgl-ii), were immunogenic. early in the infection, 24 of 84 patients (29%) who were convalescent from erythema migrans and 19 of the 35 patients (5 ... | 2009 | 19342303 |
| [isolation of tick-borne borreliosis agent from blood of patients]. | during spring-autumn period of 2006 borrelia were isolated for the first time in russia from blood of 79 patients treated in perm city clinical hospital for infectious diseases no. 1 with diagnosis "tickborne borreliosis, manifestive form with migrating erythema, localized stage". ten primary isolates (12.7% of total seeded samples) were obtained by seeding plasma samples on the bsk medium. their subsequent identification by polymerase chain reaction-restriction fragments length polymorphism rev ... | 2009 | 19340967 |
| defensin is suppressed by tick salivary gland extract during the in vitro interaction of resident skin cells with borrelia burgdorferi. | 2009 | 19340008 | |
| hla type and immune response to borrelia burgdorferi outer surface protein a in people in whom arthritis developed after lyme disease vaccination. | to investigate whether persons with treatment-resistant lyme arthritis-associated hla alleles might develop arthritis as a result of an autoimmune reaction triggered by borrelia burgdorferi outer surface protein a (ospa), the lyme disease vaccine antigen. | 2009 | 19333928 |
| inhibition of neutrophil function by two tick salivary proteins. | the saliva of hematophagous arthropods contains potent anti-inflammatory and antihemostatic activities that promote acquisition of the blood meal and enhance infection with pathogens. we have shown that polymorphonuclear leukocytes (pmn) treated with the saliva of the tick ixodes scapularis have reduced expression of beta(2) integrins, impaired pmn adherence, and reduced killing of borrelia burgdorferi, the causative agent of lyme disease. here we describe two ixodes proteins that are induced up ... | 2009 | 19332533 |
| correlation between tick density and pathogen endemicity, new hampshire. | to assess the endemicity of tick-borne pathogens in new hampshire, we surveyed adult tick vectors. pathogens were more prevalent in areas of high tick density, suggesting a correlation between tick establishment and pathogen endemicity. infection rates in ticks correlated with disease frequency in humans. | 2009 | 19331738 |
| a possible systemic rheumatic disorder in the nova scotia duck tolling retriever. | a disease complex with chronic musculoskeletal signs, including stiffness and joint pain, and to which there is a strong predisposition in the canine breed nova scotia duck tolling retriever (toller) has been recognized in sweden. the aim of this first clinical description of the disorder in tollers was to describe the clinical manifestations and laboratory findings, as well as to try to identify a possible immune-mediated background of the disease and to show the outcome of treatment in 33 toll ... | 2009 | 19331658 |
| borrelia recurrentis employs a novel multifunctional surface protein with anti-complement, anti-opsonic and invasive potential to escape innate immunity. | borrelia recurrentis, the etiologic agent of louse-borne relapsing fever in humans, has evolved strategies, including antigenic variation, to evade immune defence, thereby causing severe diseases with high mortality rates. here we identify for the first time a multifunctional surface lipoprotein of b. recurrentis, termed hcpa, and demonstrate that it binds human complement regulators, factor h, cfhr-1, and simultaneously, the host protease plasminogen. cell surface bound factor h was found to re ... | 2009 | 19308255 |
| acylated cholesteryl galactosides are specific antigens of borrelia causing lyme disease and frequently induce antibodies in late stages of disease. | borrelia burgdorferi sensu lato is the causative agent of lyme disease (ld), an infectious disease occurring in north america, europe, and asia in different clinical stages. b. burgdorferi sensu lato encompasses at least 12 species, with b. burgdorferi sensu stricto, b. garinii, and b. afzelii being of highest clinical importance. immunologic testing for ld as well as recent vaccination strategies exclusively refer to proteinaceous antigens. however, b. burgdorferi sensu stricto exhibits glycoli ... | 2009 | 19307181 |
| species of borrelia burgdorferi complex that cause borrelial lymphocytoma in france. | only about 30 cases of borrelial lymphocytoma (bl) with identification of the causative species of borrelia have been published to date, mainly from eastern or central european countries. | 2009 | 19302068 |
| the antibiotics doxycycline and minocycline inhibit the inflammatory responses to the lyme disease spirochete borrelia burgdorferi. | tetracyclines moderate inflammatory responses of various etiologies. we hypothesized that tetracyclines, in addition to their antimicrobial function, could exert control over the inflammation elicited by borrelia burgdorferi. to model systemic effects, we used the human monocytic cell line thp-1; to model effects in the central nervous system, we used rhesus monkey brain astrocytes and microglia. cells were stimulated with live or sonicated b. burgdorferi or with the lipoprotein outer surface pr ... | 2009 | 19301981 |
| infectious agents and lymphoma development: molecular and clinical aspects. | this review is focused on the role of infectious agents in the development of some lymphoma entities. associations involving bacterial infections mostly regard marginal zone b-cell lymphomas of mucosa-associated lymphoid tissue (malt)-type. some paradigmatic examples of these associations include the helicobacter pylori-related gastric malt lymphoma and the more recently reported links between chlamydophila psittaci and ocular adnexal malt lymphomas and borrelia burgdorferi and cutaneous malt ly ... | 2009 | 19298458 |
| prediction of lyme meningitis based on a logistic regression model using clinical and cerebrospinal fluid analysis: a european study. | a prediction model based on clinical and cerebrospinal fluid (csf) analysis has been proposed for the differentiation of lyme meningitis (lm) from non-lyme aseptic meningitis (nlam) in the united states. no similar model has ever been proposed for european patients. the objective of our study was to develop a prediction model to differentiate lm from nlam based on clinical and csf biologic data. | 2009 | 19295463 |
| [results of etiologic diagnosis in clinical syndrome consistent with acute and chronic borreliosis]. | borreliosis is a multisystem infection, which in the absence of adequate diagnosis and clinical management, may develop towards various clinical forms of chronic pathology. due to the heterogeneity of clinical manifestations it is known under more names: erythema migrans, lyme disease, neuroborreliosis etc. material and method: taking into account the present interest and the weight in pathology of syndromes consistent with the suspicion of a borrelia spp. infection, since 2002 we applied in cur ... | 2008 | 19295026 |
| [elevated transaminases - what to do if everything was done?]. | transaminases, gamma-gt and alcalic phosphatase are classically termed as liver enzymes, however they can be found in almost every organ. elevated levels of the transaminases alat (alanin-aminotransferase) and asat (aspartat-aminotransferase) are signs of disturbed permeability of the cells, in which these enzymes can be found. in contrast to alat, which is mainly liver-specific, the asat is found in other organs as well, e.g. heart and skeletal muscle. at a mild elevation of these enzymes a ree ... | 2009 | 19291640 |
| characterization of a novel relapsing fever spirochete in the midgut, coxal fluid, and salivary glands of the bat tick carios kelleyi. | bat ticks, carios kelleyi, from iowa were examined for the presence of relapsing fever group borreliae. a novel spirochete was characterized by dna sequence analysis of polymerase chain reaction amplicons for the 16s rrna, flab, and glpq genes in either triturated tick pools or single ticks. all loci and the concatenated dna sequence of 3,289 bases identified the carios bacterium as a relapsing fever spirochete most closely related to, but distinct from, borrelia turicatae. spirochetes reactive ... | 2009 | 19281412 |
| langerhans cell deficiency impairs ixodes scapularis suppression of th1 responses in mice. | ixodes scapularis ticks transmit a number of human pathogens, including the lyme disease spirochete borrelia burgdorferi. i. scapularis suppresses host immunity in the skin to promote feeding and systemically skew t-helper (th)-cell differentiation toward th2 cells in secondary lymphoid organs. although components of tick saliva are known to influence th-cell polarization, the mechanism whereby tick feeding in the skin modulates regional and systemic th-cell responses is unknown. in this study, ... | 2009 | 19273564 |
| molecular characterization of the tick-borrelia interface. | spirochetes in the genus borrelia are responsible for tick-borne relapsing fever and lyme disease. borrelia-tick interactions are highly specific as each species of borrelia is only transmitted by one or a few closely related species of ticks. borrelia colonize the gut or salivary glands of ticks. several borrelia genes required for tick colonization or transmission have been identified. borrelia genes required for transmission are induced by a pathway controlled by the alternate sigma factors r ... | 2009 | 19273256 |
| the use of harvested white-tailed deer (odocoileus virginianus) and geographic information system (gis) methods to characterize distribution and locate spatial clusters of borrelia burgdorferi and its vector ixodes scapularis in indiana. | ixodes scapularis (say) is the vector for borrelia burgdorferi (bb) the causative agent of lyme disease (ld). the increased number and presence of ticks in the environment pose a significant health risk to people and many domestic animals including dogs, cats, and horses. this study characterized the distribution and expansion of i. scapularis and bb and identified areas of increased risk of ld transmission in indiana using geographical information systems (gis) and spatial analysis. a cross-sec ... | 2009 | 19272000 |
| detection of anaplasma phagocytophilum and borrelia burgdorferi sensu lato in dogs in the czech republic. | the aim of this study is to present molecular, serologic, and clinical findings for dogs that were naturally infected with anaplasma phagocytophilum or borrelia burgdorferi sensu lato (s. l.) in the czech republic. this data can provide information relevant to human infection. in total, blood samples from 296 dogs and 118 engorged ticks were examined. samples were tested for a. phagocytophilum using polymerase chain reaction (pcr) amplification, nested pcr, and direct sequencing of the 16s rdna, ... | 2009 | 19271998 |
| a borreliacidal factor in amblyomma americanum saliva is associated with phospholipase a2 activity. | previous work in our laboratory described the in vitro killing of borrelia burgdorferi when co-cultured with saliva from adult amblyomma americanum. borreliacidal activity was not evident using ixodes scapularis saliva. mixing trypsin with saliva eliminated the borreliacidal activity of a. americanum saliva, while incorporating a trypsin inhibitor restored all borreliacidal activity, indicating this factor was of protein or peptide origin. one-dimensional page indicated at least 7 major protein ... | 2009 | 19271281 |
| first detection and molecular identification of borrelia burgdorferi-like spirochetes in ixodes granulatus ticks collected on kinmen island of taiwan. | borrelia burgdorferi-like spirochetes were detected for the first time in ixodes granulatus ticks collected in taiwan. the genetic identities of these detected spirochetes were determined by analyzing the gene sequences amplified by a genospecies-specific polymerase chain reaction assay based on the outer surface protein a (ospa) gene of b. burgdorferi sensu lato. phylogenetic analysis reveals that the sequence similarity of these spirochetes, ranging from 90.5% to 98.7% is highly homogeneous wi ... | 2009 | 19270287 |
| intergenic regions of borrelia plasmids contain phylogenetically conserved rna secondary structure motifs. | borrelia species are unusual in that they contain a large number of linear and circular plasmids. many of these plasmids have long intergenic regions. these regions have many fragmented genes, repeated sequences and appear to be in a state of flux, but they may serve as reservoirs for evolutionary change and/or maintain stable motifs such as small rna genes. | 2009 | 19267927 |
| a chromosomally encoded virulence factor protects the lyme disease pathogen against host-adaptive immunity. | borrelia burgdorferi, the bacterial pathogen of lyme borreliosis, differentially expresses select genes in vivo, likely contributing to microbial persistence and disease. expression analysis of spirochete genes encoding potential membrane proteins showed that surface-located membrane protein 1 (lmp1) transcripts were expressed at high levels in the infected murine heart, especially during early stages of infection. mice and humans with diagnosed lyme borreliosis also developed antibodies against ... | 2009 | 19266024 |
| local production of ifn-gamma by invariant nkt cells modulates acute lyme carditis. | the lyme disease spirochete borrelia burgdorferi is the only known human pathogen that directly activates invariant nkt (inkt) cells. the number and activation kinetics of inkt cells vary greatly among different strains of mice. we now report the role of the inkt cell response in the pathogenesis of lyme disease using c57bl/6 mice, a strain with optimal inkt cell activation that is resistant to the development of spirochetal-induced inflammation. during experimental infection of b6 mice with b. ... | 2009 | 19265151 |
| a longitudinal study of the prevalence of borreliae in ticks in the urban locality of brno-pisárky, czech republic. | 2008 | 19263859 | |
| the borrelia burgdorferi flab promoter has an extended -10 element and includes a t-rich -35/-10 spacer sequence that is essential for optimal activity. | in this study, we investigated the functional elements of the flab promoter of borrelia burgdorferi. promoter function was examined in a high-passage variant of strain jd1 using a set of 5' deletions and mutations within the flab promoter. expression from the modified flab promoters was assayed using the gene for green fluorescent protein (gfp) as a reporter. although the -35 element of the promoter stimulated promoter activity, its disruption did not negate expression. sequences upstream of the ... | 2009 | 19260969 |
| laboratory data in children with lyme neuroborreliosis, relation to clinical presentation and duration of symptoms. | the occurrence of igm and igg antibodies against borrelia burgdoferi in serum and cerebrospinal fluid (csf) and intrathecal synthesis of antibodies (antibody index) were studied in relation to clinical presentation and the duration of symptoms before diagnosis in 146 children diagnosed with neuroborreliosis. lymphocytic meningitis was demonstrated in 141 of these children. levels of white blood cells (wbc) and protein in csf correlated significantly to numbers of d with symptoms. children were d ... | 2009 | 19253089 |
| climate and tick seasonality are predictors of borrelia burgdorferi genotype distribution. | the blacklegged tick, ixodes scapularis, is of significant public health importance as a vector of borrelia burgdorferi, the agent of lyme borreliosis. the timing of seasonal activity of each immature i. scapularis life stage relative to the next is critical for the maintenance of b. burgdorferi because larvae must feed after an infected nymph to efficiently acquire the infection from reservoir hosts. recent studies have shown that some strains of b. burgdorferi do not persist in the primary res ... | 2009 | 19251900 |
| the borrelia burgdorferi outer-surface protein erpx binds mammalian laminin. | the lyme disease spirochaete, borrelia burgdorferi, can invade and persistently infect its hosts' connective tissues. we now demonstrate that b. burgdorferi adheres to the extracellular matrix component laminin. the surface-exposed outer-membrane protein erpx was identified as having affinity for laminin, and is the first laminin-binding protein to be identified in a lyme disease spirochaete. the adhesive domain of erpx was shown to be contained within a small, unstructured hydrophilic segment a ... | 2009 | 19246757 |
| local borrelia burgdorferi sensu stricto and borrelia afzelii strains in a single mixed antigen improves western blot sensitivity. | this study evaluates the use of local borrelia burgdorferi sensu stricto and borrelia afzelii strains in a single mixed antigen for in-house igg western blots in the routine diagnostic setting by comparing it with the current in-house protocol. | 2009 | 19240047 |
| [acute encephalitis. neuropsychiatric manifestations as expression of influenza virus infection]. | the aim is to review the encephalitis in infants and adolescents as well as its etiology, clinical manifestation, epidemiology, physiopathology, diagnostic methods and treatment, and the neuropsyquiatric signs appearing an influenza epidemy. encephalitis is an inflammation of the central nervous system (cns) which involves the brain. the clinical manifestations usually are: headache, fever and confusional stage. it could also be manifested as seizures, personality changes, or psiqyiatric symptom ... | 2009 | 19240010 |
| detection and quantification of lyme spirochetes using sensitive and specific molecular beacon probes. | lyme disease, caused by borrelia burgdorferi, affects a large number of people in both the usa and europe. the mouse is a natural host for this spirochete and is widely used as a model system to study lyme pathogenesis mechanisms. since disease manifestations often depend upon the spirochete burden in a particular tissue, it is critical to accurately measure the bacterial number in infected tissues. the current methods either lack sensitivity and specificity (sybr green), or require independent ... | 2009 | 19239692 |
| [pediatric dermatology. new aspects of bacterial skin infections in children]. | we review important aspects of bacterial skin diseases in children, most commonly caused by staphylococcus aureus, group a beta-hemolytic streptococci (streptococcus pyogenes) and borrelia burgdorferi. for early diagnosis of lyme borreliosis in children it is important to be familiar with the variable clinical presentation of erythema migrans and early hematogenic dissemination with multiple erythemata migrantia. treatment of impetigo in children requires consideration of concomitant diseases, t ... | 2009 | 19238341 |
| [fibrous juxtaarticular nodules]. | 2009 | 19232260 | |
| [a curious case of livedo]. | 2009 | 19232258 | |
| indices of urine n-acetyl-beta-d-glucosaminidase and gamma-glutamyl transpeptidase activities in clinically normal adult dogs. | to establish reference ranges for indices of urine n-acetyl-b-d-glucosaminidase (nag) and gamma-glutamyl transpeptidase (ggt) activities in clinically normal adult dogs. | 2009 | 19231965 |
| antimicrobial activity of three tick defensins and four mammalian cathelicidin-derived synthetic peptides against lyme disease spirochetes and bacteria isolated from the midgut. | in this study, chemically synthesized tick defensins and cathelicidin-derived mammalian peptides were used to investigate the activity spectrum against borrelia garinii and symbiotic stenotrophomonas maltophila. synthetic tick defensins showed antimicrobial activity against staphylococcus aureus but not b. garinii and s. maltophila. mammalian peptides which have cationic property similar to tick defensins, showed antimicrobial activity similar to tick defensins. the antimicrobial peptides in tic ... | 2009 | 19229642 |
| normal pressure hydrocephalus or neuroborreliosis? | an 80-year-old woman presented with progressive cognitive decline and with a 6-month history of gait ataxia. brain mri depicted enlarged ventricles and periventricular lesions. clinical improvement after csf spinal tap test suggested a normal pressure hydrocephalus syndrome. but csf pleocytosis with activated lymphocytes and plasma cells and intrathecal borrelia burgdorferi specific antibody production led to the diagnosis of active lyme neuroborreliosis. clinical symptoms of nph resolved after ... | 2009 | 19225737 |
| a manganese transporter, bb0219 (bmta), is required for virulence by the lyme disease spirochete, borrelia burgdorferi. | borrelia burgdorferi (bb), the causative agent of lyme disease, is transmitted to mammalian hosts through an arthropod (tick) vector. to establish infection, bb must acquire essential nutrients, including transition metals, from its mammalian and tick hosts. thus far, no metal transporter has been identified in bb. here, we report the identification of the first metal transporter, bmta (bb0219), in bb. bmta-deficient mutants of virulent bb were readily generated, and the mutants grew slightly sl ... | 2009 | 19218460 |
| borrelia burgdorferi bba74 is expressed exclusively during tick feeding and is regulated by both arthropod- and mammalian host-specific signals. | although bba74 initially was described as a 28-kda virulence-associated outer-membrane-spanning protein with porin-like function, subsequent studies revealed that it is periplasmic and downregulated in mammalian host-adapted spirochetes. to further elucidate the role of this protein in the borrelia burgdorferi tick-mammal cycle, we conducted a thorough examination of its expression profile in comparison with the profiles of three well-characterized, differentially expressed borrelial genes (ospa ... | 2009 | 19218390 |
| detailed analysis of sequence changes occurring during vlse antigenic variation in the mouse model of borrelia burgdorferi infection. | lyme disease borrelia can infect humans and animals for months to years, despite the presence of an active host immune response. the vls antigenic variation system, which expresses the surface-exposed lipoprotein vlse, plays a major role in b. burgdorferi immune evasion. gene conversion between vls silent cassettes and the vlse expression site occurs at high frequency during mammalian infection, resulting in sequence variation in the vlse product. in this study, we examined vlse sequence variati ... | 2009 | 19214205 |
| [radiculalgia revealing lyme disease]. | 2008 | 19213505 | |
| louse-borne relapsing fever in ethiopian children: experience of a rural hospital. | we describe the epidemiological and clinical aspects of louse-borne relapsing fever (lbrf) in a series of children attending in a rural hospital in ethiopia during 1997-2007. from a total of 249 cases of lbrf, 154 (61.4%) were children (<15 years). the most frequent symptoms were: fever, headache, dizziness and musculoskeletal pains. the overall case fatality rate was 2.4 (10% for patients <1.1 years; 3.4% for 1.1 to 4.0 years; and 0% >4.0 years [p = 0.05]). the mortality in children was less th ... | 2009 | 19211422 |
| rrp1, a cyclic-di-gmp-producing response regulator, is an important regulator of borrelia burgdorferi core cellular functions. | two-component systems (tcs) are universal among bacteria and play critical roles in gene regulation. our understanding of the contributions of tcs in the biology of the borrelia is just now beginning to develop. borrelia burgdorferi, a causative agent of lyme disease, harbours a tcs comprised of open reading frames (orfs) bb0419 and bb0420. bb0419 encodes a response regulator designated rrp1, and bb0420 encodes a hybrid histidine kinase-response regulator designated hpk1. rrp1, which contains a ... | 2009 | 19210621 |
| comparative cryo-electron tomography of pathogenic lyme disease spirochetes. | spirochetes of the borrelia burgdorferi sensu lato group, the causative agents of lyme borreliosis, exhibit a complex biology evolved in its zoonotic cycle. cryo-electron tomography was used to investigate structural features of three species, b. burgdorferi, b. garinii and b. afzelii, known to cause different clinical manifestations in humans. all three organisms revealed an overall similar architecture and showed different numbers of periplasmic flagellar filaments, polar periplasmic void regi ... | 2009 | 19210619 |
| borrelia burgdorferi ebfc defines a newly-identified, widespread family of bacterial dna-binding proteins. | the lyme disease spirochete, borrelia burgdorferi, encodes a novel type of dna-binding protein named ebfc. orthologs of ebfc are encoded by a wide range of bacterial species, so characterization of the borrelial protein has implications that span the eubacterial kingdom. the present work defines the dna sequence required for high-affinity binding by ebfc to be the 4 bp broken palindrome gtnac, where 'n' can be any nucleotide. two high-affinity ebfc-binding sites are located immediately 5' of b. ... | 2009 | 19208644 |
| rrp2, a sigma54-dependent transcriptional activator of borrelia burgdorferi, activates rpos in an enhancer-independent manner. | rrp2 is the sole sigma(54)-dependent transcriptional activator present in the borrelia burgdorferi genome. we showed that recombinant rrp2 binds to dna in a sequence-nonspecific manner. during infection, rrp2 activates sigma(54)-dependent rpos expression without an apparent upstream enhancer element commonly associated with other sigma(54)-dependent transcriptional activators. | 2009 | 19201806 |
| detection of borrelia burgdorferi and borrelia lonestari in birds in tennessee. | lyme disease in the united states is caused by the bacterial spirochete borrelia burgdorferi s.s. (johnson, schmid, hyde, steigerwalt, and brenner), which is transmitted by tick vectors ixodes scapularis (say) and i. pacificus (cooley and kohls). borrelia lonestari, transmitted by the tick amblyomma americanum l., may be associated with a related syndrome, southern tick-associated rash illness (stari). borrelia lonestari sequences, reported primarily in the southeastern states, have also been de ... | 2009 | 19198527 |
| [spatial and temporal variability of ixodes ricinus and ixodes persulcatus infection with the lyme disease agent in moscow region]. | analysis of long-term data on the infection rated of the taxonomic complex borrelia burgdorferi s. l. in ixodes persulcatus and i. ricinus from moscow region is carried out. more than 14 000 tick specimens were examined by dark-field microscopy and 704 specimens were investigated by pcr. two borrelia genospicies, b. afzelii (6.3%) and b. garinii (20%) proved the most prevalent. the genospecies could form a mixtinfection (11%). borrelia miyamotoi, b. valaisiana, and b. burgdorferi s. s. were also ... | 2008 | 19198169 |
| borrelia infection and pityriasis rosea. | 2009 | 19197556 | |
| dermatologic changes induced by repeated ixodes scapularis bites and implications for prevention of tick-borne infection. | previous studies in rodents and people have demonstrated that repeated tick exposure is associated with reduced borrelia burgdorferi transmission but the mechanism of prevention remains unclear. we examined the acute histopathologic reactions to initial and repeated ixodes scapularis bites in balb/c mice and in people. skin biopsies of balb/c mice infested for the first time by i. scapularis nymphs revealed vascular dilatation and an accumulation of inflammatory cells adjacent to the bite site b ... | 2009 | 19196014 |
| prolonged synovitis in pediatric lyme arthritis cannot be predicted by clinical or laboratory parameters. | ten percent of lyme arthritis (la) patients have continued synovitis despite antimicrobial therapy. the current study was designed to (1) investigate predictors of prolonged disease and (2) further define natural history of pediatric la. medical records of 94 children fulfilling centers for disease control criteria for lyme disease were reviewed, classified into groups according to duration of synovitis, and spss statistical software was used for analysis. thirty-nine percent required >6 months ... | 2009 | 19194735 |
| five cases of paralytic strabismus as a rare feature of lyme disease. | lyme borreliosis is the most common human tick-borne disease in the northern hemisphere. the prevalence of lyme borreliosis is estimated to be 20-100 cases per 100,000 persons in the united states and 100-155 cases per 100,000 persons in europe [ 1 , 2 ]. it is caused by the spirochete borrelia burgdorferi sensu lato [ 2 , 3 ]. the course of clinical manifestations is thought to occur in 3 stages: early, disseminated, and chronic [ 2 , 4 ]. lyme borreliosis can cause a variety of ocular manifest ... | 2009 | 19193112 |
| local intra-arterial thrombolysis in a 4-year-old male with vertebrobasilar artery thrombosis. | we report the case of a 4-year-old male with vertebrobasilar artery thrombosis for which he was treated with local intra-arterial urokinase 60 hours after onset of symptoms. initially the patient had dysarthria and dysphagia. brain magnetic resonance imaging (mri) in a community hospital showed abnormalities in the pons, and vertebrobasilar artery thrombosis was suspected. the patient was transferred to the university hospital because of neurological deterioration. just before thrombolysis, his ... | 2009 | 19191847 |
| arthritogenicity of borrelia burgdorferi and borrelia garinii: comparison of infection in mice. | arthritogenicity, as determined by joint swelling and synovial histology, was compared between or within two borrelia genospecies that cause lyme arthritis in humans. the spirochete burden in bladder tissue (a site of spirochete persistence) was documented by quantitative polymerase chain reaction, and immune responses were analyzed. in c3h/hej mice, three b. burgdorferi isolates and two of the three b. garinii isolates induced severe arthritis and swelling. previous designation as invasive or n ... | 2009 | 19190223 |
| molecular detection of borrelia bissettii dna in serum samples from patients in the czech republic with suspected borreliosis. | until recently, three spirochete genospecies were considered to be the causative agents of lyme borreliosis (lb) in europe: borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii. however, the dna of borrelia valaisiana, borrelia lusitaniae, borrelia spielmanii and borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of lb. molecular analysis of 12 selected serum samples collected in the regio ... | 2009 | 19187198 |
| molecular analysis of ixodes granulatus, a possible vector tick for borrelia burgdorferi sensu lato in taiwan. | the genetic identity of ixodes granulatus ticks was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 19 strains of ticks representing seven species of ixodes and two outgroup species (rhipicephalus sanguineus and haemaphysalis inermis). four major clades could be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. all these i. granul ... | 2009 | 19184580 |
| in vitro activity of tigecycline against multiple strains of borrelia burgdorferi. | to compare the antimicrobial activity of tigecycline and doxycycline against multiple isolates of borrelia burgdorferi. | 2009 | 19182236 |
| [isolation and identification of borrelia burgdorferi sensu lato in leye county, guangxi province]. | 2008 | 19173980 | |
| [detection of co-infection with lyme spirochetes and spotted fever group rickettsiae in a group of haemaphysalis longicornis]. | the present study was conducted to investigate the infection of lyme disease, spotted fever, ehrlichiosis (anaplasmosisin) in wild animals and ticks in the mountain areas of zhejiang province. | 2008 | 19173967 |
| holliday junction formation by the borrelia burgdorferi telomere resolvase, rest: implications for the origin of genome linearity. | spirochetes of the genus borrelia include the causative agents of lyme disease and relapsing fever. they possess unusual, highly segmented genomes composed mostly of linear replicons with covalently closed hairpin telomeres. the telomeres are formed from inverted repeat replicated telomere junctions (rtels) by the telomere resolvase, rest. rest uses a reaction mechanism with similarities to that employed by the type ib topoisomerases and tyrosine recombinases. here, we report that the relationsh ... | 2009 | 19170885 |