Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antibiotic resistance of gram negatives isolates from loggerhead sea turtles (caretta caretta) in the central mediterranean sea. | previous studies on fish and marine mammals support the hypothesis that marine species harbor antibiotic resistance and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to assess the resistance to antimicrobial agents of gram negative strains isolated from loggerhead sea turtles (carettacaretta). oral and cloacal swabs from 19 live-stranded loggerhead sea turtles, with hooks fixed into the gut, were analyzed. the antimicrobial resistance ... | 2009 | 19473669 |
| copper uptake by pseudomonas aeruginosa isolated from infected burn patients. | pseudomonas aeruginosa was isolated from infected burn patients and characterized by standard biochemical tests. the in vitro copper uptake was compared between this isolated pathogenic strain and two non-pathogenic control strains of gram positive bacteria bacillus thuringiensis strain israelis as well as gram negative bacteria enterobacter aerogenes. maximum copper uptake of 470 ppm/g biomass was obtained by p. aeruginosa strain, while the control strains b. thuringiensis and enterobacter aero ... | 2009 | 19484296 |
| effect of 2,4-dinitrotoluene on the anaerobic bacterial community in marine sediment. | to study the impact of added 2,4-dinitrotoluene (dnt) on the anaerobic bacterial community in marine sediment collected from an unexploded ordnance dumping site in halifax harbour. | 2009 | 19486208 |
| membrane-electrode assembly enhances performance of a microbial fuel cell type biological oxygen demand sensor. | a membrane-electrode assembly (mea) was applied to a microbial fuel cell (mfc) type biological oxygen demand (bod) sensor and the performance of the sensor was assessed. to establish the optimal conditions for mea fabrication, platinum-catalysed carbon cloth cathodic electrodes were assembled with cation exchange membranes under various temperatures and pressures. by analysing coulombs from the mfcs, it could be determined that the optimal hot-pressing conditions were 120 degrees c and 150 kg cm ... | 2009 | 19492544 |
| motx and moty are required for flagellar rotation in shewanella oneidensis mr-1. | the single polar flagellum of shewanella oneidensis mr-1 is powered by two different stator complexes, the sodium-dependent pomab and the proton-driven motab. in addition, shewanella harbors two genes with homology to motx and moty of vibrio species. in vibrio, the products of these genes are crucial for sodium-dependent flagellar rotation. resequencing of s. oneidensis mr-1 moty revealed that the gene does not harbor an authentic frameshift as was originally reported. mutational analysis demons ... | 2009 | 19502394 |
| turnover of quorum sensing signal molecules modulates cross-kingdom signalling. | n-acylhomoserine lactone (ahl) quorum-sensing molecules modulate the swimming behaviour of zoospores of the macroalga ulva to facilitate the location of bacterial biofilms. here we show that the intertidal surfaces colonized by ulva are dominated by alphaproteobacteria, particularly the rhodobacteraceae family, and the bacteroidetes family flavobacteriaceae, and that this diverse assemblage both produces and degrades ahls. n-acylhomoserine lactones could also be extracted from the surfaces of pe ... | 2009 | 19508552 |
| a novel packaging method with a dissolving co(2) headspace combined with organic acids prolongs the shelf life of fresh salmon. | the aim of this study was to evaluate a novel packaging method for fresh fish and to determine its effect on the bacterial growth in fresh salmon. fresh salmon was packed with a small amount of 100% co(2) (gas/product ratio 0.2/1.0 v/v) and a brine solution containing various combinations of citric acid (3% w/w, ph 5), acetic acid (1% w/w, ph 5) and cinnamaldehyde (200 microg/ml). total bacterial counts, counts of sulphur reducing bacteria, lactic acid bacteria and enterobacteriaceae as well as ... | 2009 | 19523706 |
| the agglutination protein agga from shewanella oneidensis mr-1 is a tolc-like protein and forms active channels in vitro. | type i secretion systems (tiss) are associated with the virulence of gram-negative bacteria and the secretion of pathogenic molecular determinants. the shewanella oneidensis mr-1 outer-membrane protein agga is part of a tiss. recombinant agga expressed in escherichia coli as inclusion bodies can be efficiently refolded in vitro, and can form active channel-tunnels as shown by proteoliposome swelling assays and electrophysiological measurements in lipid bilayers. structure-based sequence alignmen ... | 2009 | 19524550 |
| effects of oxytetracycline and sulfachloropyridazine residues on the reductive activity of shewanella decolorationis s12. | effects of oxytetracycline (otc) and sulfachloropyridazine (scp), two of the widely used antibiotics in livestock production, on beneficial environmental microorganisms were studied. shewanella decolorationis s12 was selected as the target bacteria for the role in reduction of fe(iii) and dye under anaerobic conditions. the results showed that the antibiotics significantly inhibited fe(iii) reduction and dye decoloration in the reduction system. the rates of fe(ii) formed (-r) were 3.6 and 0.2 m ... | 2009 | 19527026 |
| combined effect of an oxygen absorber and oregano essential oil on shelf life extension of rainbow trout fillets stored at 4 degrees c. | in the present study the combined effect of an o2 absorber and oregano essential oil (0.4% v/w) on shelf life extension of rainbow trout fillets (onchorynchus mykiss) stored under refrigeration (4 degrees c) was investigated. the study was based on microbiological [tvc, pseudomonas spp., lactic acid bacteria, h2s-producing bacteria including shewanella putrefaciens, enterobacteriaceae and clostridium spp.), physicochemical (ph, pv, tba, tvbn and drip loss) and sensory (odor, taste) changes occur ... | 2009 | 19527835 |
| limitations and benefits of arisa intra-genomic diversity fingerprinting. | monitoring diversity changes and contamination in mixed cultures and simple microcosms is challenged by fast community structure dynamics, and the need for means allowing fast, cost-efficient and accurate identification of microorganisms at high phylogenetic resolution. the method we explored is a variant of automated rrna intergenic spacer analysis based on intra-genomic diversity fingerprinting (arisa-igdf), and identifies phylotypes with multiple 16s-23s rrna gene intergenic transcribed space ... | 2009 | 19538993 |
| anaerobic respiration of elemental sulfur and thiosulfate by shewanella oneidensis mr-1 requires psra, a homolog of the phsa gene of salmonella enterica serovar typhimurium lt2. | shewanella oneidensis mr-1, a facultatively anaerobic gammaproteobacterium, respires a variety of anaerobic terminal electron acceptors, including the inorganic sulfur compounds sulfite (so3(2-)), thiosulfate (s2o3(2-)), tetrathionate (s4o6(2-)), and elemental sulfur (s(0)). the molecular mechanism of anaerobic respiration of inorganic sulfur compounds by s. oneidensis, however, is poorly understood. in the present study, we identified a three-gene cluster in the s. oneidensis genome whose trans ... | 2009 | 19542325 |
| kinetic characterization of omca and mtrc, terminal reductases involved in respiratory electron transfer for dissimilatory iron reduction in shewanella oneidensis mr-1. | we have used scaling kinetics and the concept of kinetic competence to elucidate the role of hemeproteins omca and mtrc in iron reduction by shewanella oneidensis mr-1. second-order rate constants for omca and mtrc were determined by single-turnover experiments. for soluble iron species, a stopped-flow apparatus was used, and for the less reactive iron oxide goethite, a conventional spectrophotometer was used to measure rates. steady-state experiments were performed to obtain molecular rate cons ... | 2009 | 19542342 |
| on-line digestion system for protein characterization and proteome analysis. | an efficient on-line digestion system that reduces the number of sample manipulation steps has been demonstrated for high-throughput proteomics. by incorporating a pressurized sample loop into a liquid chromatography-based separation system, both sample and enzyme (e.g., trypsin) can be simultaneously introduced to produce a complete, yet rapid digestion. both standard proteins and a complex shewanella oneidensis global protein extract were digested and analyzed using the automated online pressu ... | 2008 | 19551971 |
| evaluation of a high-intensity focused ultrasound-immobilized trypsin digestion and 18o-labeling method for quantitative proteomics. | a new method that uses immobilized trypsin concomitant with ultrasonic irradiation results in ultrarapid digestion and more thorough (18)o labeling for quantitative protein comparisons. the method was reproducible and provided effective digestions within <1 min with lower amounts of enzyme, compared to traditional methods. this method was demonstrated for digestion of both simple and complex protein mixtures, including bovine serum albumin, a global proteome extract from the bacteria shewanella ... | 2009 | 19555078 |
| unexpected chemoreceptors mediate energy taxis towards electron acceptors in shewanella oneidensis. | shewanella oneidensis uses a wide range of terminal electron acceptors for respiration. in this study, we show that the chemotactic response of s. oneidensis to anaerobic electron acceptors requires functional electron transport systems. deletion of the genes encoding dimethyl sulphoxide and trimethylamine n-oxide reductases, or inactivation of these molybdoenzymes as well as nitrate reductase by addition of tungstate, abolished electron acceptor taxis. moreover, addition of nigericin prevented ... | 2009 | 19555457 |
| engineering of monomeric fk506-binding protein 22 with peptidyl prolyl cis-trans isomerase. importance of a v-shaped dimeric structure for binding to protein substrate. | fk506-binding protein 22 (fkbp22) from the psychrotrophic bacterium shewanella sp. sib1 is a homodimeric protein with peptidyl prolyl cis-trans isomerase (ppiase) (ec 5.2.1.8) activity. each monomer consists of 205 amino acid residues. according to a tertiary model, sib1 fkbp22 assumes a v-shaped structure, in which two monomers interact with each other at their n-termini. each monomer consists of an n-terminal domain with a dimerization core and a c-terminal catalytic domain, which are separate ... | 2009 | 19558490 |
| remediation of trichloroethylene by bio-precipitated and encapsulated palladium nanoparticles in a fixed bed reactor. | trichloroethylene is a toxic and recalcitrant groundwater pollutant. palladium nanoparticles bio-precipitated on shewanella oneidensis were encapsulated in polyurethane, polyacrylamide, alginate, silica or coated on zeolites. the reactivity of these bio-pd beads and zeolites was tested in batch experiments and trichloroethylene dechlorination followed first order reaction kinetics. the calculated k-values of the encapsulated catalysts were a factor of six lower compared to non-encapsulated bio-p ... | 2009 | 19560796 |
| shewanella marina sp. nov., isolated from seawater. | a motile, rod-shaped, pale-brown-pigmented bacterium, designated strain c4t, was isolated from seawater collected from the south sea (republic of korea). cells were gram-negative, facultatively anaerobic, and catalase- and oxidase-positive. the major fatty acids were summed feature 3 (c16:1omega7c and/or iso-c15:0 2-oh; 19.4%), c16:0 (16.3%), c17:1omega8c (9.5%) and iso-c15:0 (7.7%). the dna g+c content was 40.8 mol%. a phylogenetic tree based on 16s rrna gene sequences showed that strain c4t fo ... | 2009 | 19567571 |
| the role of 4-hydroxyphenylpyruvate dioxygenase in enhancement of solid-phase electron transfer by shewanella oneidensis mr-1. | we hypothesized that shewanella oneidensis mr-1, a model dissimilatory metal-reducing bacterium, could utilize environmentally relevant concentrations of tyrosine to produce pyomelanin for enhanced fe(iii) oxide reduction. because homogentisate is an intermediate of the tyrosine degradation pathway, and a precursor of a redox-cycling metabolite, pyomelanin, we evaluated the process of homogentisate production by s. oneidensis mr-1, in order to identify the key steps involved in pyomelanin produc ... | 2009 | 19573203 |
| impedance spectroscopy as a tool for non-intrusive detection of extracellular mediators in microbial fuel cells. | endogenously produced, diffusible redox mediators can act as electron shuttles for bacterial respiration. accordingly, the mediators also serve a critical role in microbial fuel cells (mfcs), as they assist extracellular electron transfer from the bacteria to the anode serving as the intermediate electron sink. electrochemical impedance spectroscopy (eis) may be a valuable tool for evaluating the role of mediators in an operating mfc. eis offers distinct advantages over some conventional analyti ... | 2009 | 19585525 |
| exafs and hrtem evidence for as(iii)-containing surface precipitates on nanocrystalline magnetite: implications for as sequestration. | arsenic sorption onto iron oxide spinels such as magnetite could contribute to immobilization of arsenite (aso3(3-)), the reduced, highly toxic form of arsenic in contaminated anoxic groundwaters, as well as to putative remediation processes. nanocrystalline magnetite (<20 nm) is known to exhibit higher efficiency for arsenite sorption than larger particles, sorbing as much as approximately 20 micromol/m2 of arsenite. to improve our understanding of this process, we investigated the molecular le ... | 2009 | 19601563 |
| intermittent energy harvesting improves the performance of microbial fuel cells. | in this study, we compare the efficiencies of harvesting energy from microbial fuel cells (mfc) using two modes of operation: (1) continuous-passing the current through an electrical load-and (2) intermittent-first accumulating the energy in a capacitor and then discharging it through the load. each of these two modes of operation has advantages and disadvantages: the first mode of operation allows the continuous powering of low-power-consuming devices, and the second mode of operation allows th ... | 2009 | 19603683 |
| invariability of central metabolic flux distribution in shewanella oneidensis mr-1 under environmental or genetic perturbations. | an environmentally important bacterium with versatile respiration, shewanella oneidensis mr-1, displayed significantly different growth rates under three culture conditions: minimal medium (doubling time approximately 3 h), salt stressed minimal medium (doubling time approximately 6 h), and minimal medium with amino acid supplementation (doubling time approximately 1.5 h). (13)c-based metabolic flux analysis indicated that fluxes of central metabolic reactions remained relatively constant under ... | 2009 | 19610125 |
| shewanella chilikensis sp. nov., a moderately alkaliphilic gammaproteobacterium isolated from a lagoon. | a gram-negative, motile, rod-shaped, facultatively anaerobic bacterium was isolated from sediment of chilika lagoon, a brackish water lagoon in india. the strain, designated jc5(t), was able to grow in the presence of 0-8.0 % nacl and at ph 7.0-10.0. the isolate was positive for oxidase and catalase and exhibited alpha-haemolysis. the major fatty acids were iso-c(15 : 0) (18.3 %), c(16 : 0) (11.3 %), c(17 : 1)omega8c (13.1 %) and a summed feature, c(16 : 1)omega7c and/or c(16 : 1)omega6c (15.1 % ... | 2009 | 19643885 |
| electrochemical measurement of electron transfer kinetics by shewanella oneidensis mr-1. | shewanella oneidensis strain mr-1 can respire using carbon electrodes and metal oxyhydroxides as electron acceptors, requiring mechanisms for transferring electrons from the cell interior to surfaces located beyond the cell. although purified outer membrane cytochromes will reduce both electrodes and metals, s. oneidensis also secretes flavins, which accelerate electron transfer to metals and electrodes. we developed techniques for detecting direct electron transfer by intact cells, using turnov ... | 2009 | 19661057 |
| microfabricated microbial fuel cell arrays reveal electrochemically active microbes. | microbial fuel cells (mfcs) are remarkable "green energy" devices that exploit microbes to generate electricity from organic compounds. mfc devices currently being used and studied do not generate sufficient power to support widespread and cost-effective applications. hence, research has focused on strategies to enhance the power output of the mfc devices, including exploring more electrochemically active microbes to expand the few already known electricigen families. however, most of the mfc de ... | 2009 | 19668333 |
| microbial reduction of intragrain u(vi) in contaminated sediment. | the accessibility of precipitated, intragrain u(vi) in a contaminated sediment to microbial reduction was investigated to ascertain geochemical and microscopic transport phenomena controlling u(vi) bioavailability. the sediment was collected from the u.s. department of energy hanford site, and contained uranyl precipitates within the mm-sized granitic lithic fragments in the sediment. bioreduction was investigated in a culture of a dissimilatory metal-reducing bacterium, shewanellea oneidensis s ... | 2009 | 19673287 |
| shewanella xiamenensis sp. nov., isolated from coastal sea sediment. | a gram-negative, motile, rod-shaped bacterium, strain s4(t), was isolated from coastal sediment collected off xiamen, china. the physiological and biochemical features of strain s4(t), determined using the api 20ne, api zym and biolog gn2 systems, were similar to those of members of the genus shewanella. phylogenetic analyses based on 16s rrna and gyrb gene sequences placed strain s4(t) in the genus shewanella, and it was most closely related to shewanella oneidensis and related species. dna-dna ... | 2010 | 19684319 |
| the effect of flavin electron shuttles in microbial fuel cells current production. | the effect of electron shuttles on electron transfer to microbial fuel cell (mfc) anodes was studied in systems where direct contact with the anode was precluded. mfcs were inoculated with shewanella cells, and flavins used as the electron shuttling compound. in mfcs with no added electron shuttles, flavin concentrations monitored in the mfcs' bulk liquid increased continuously with fmn as the predominant flavin. the maximum concentrations were 0.6 microm for flavin mononucleotide and 0.2 microm ... | 2010 | 19697021 |
| biodegradation of anthraquinone dyes by shewanella sp. ntou1 under anaerobic conditions. | shewanella sp. ntou1 was able to decolorize a range of anthraquinone dyes [reactive blue 4 (rb4), reactive blue 19 (rb19), mordant red 11 (mr11), disperse red 15 (dr15), and disperse blue 3 (db3)] under anaerobic conditions. by supplementing the medium with formate and ferric citrate as the electron donor and acceptor, respectively and cultivating it under the optimum ph (8-9) and temperature (45 degrees c), this strain could decolorize these dyes (1,000 mg/l) at the initial color removal rates ... | 2009 | 19700827 |
| redox transformation and biogeochemical interaction of heavy metals in korean soil using different treatment columns in the presence of shewanella sp. | the redox transformation and biogeochemical interactions of heavy metals in soil was examined using facultative anaerobic bacterium shewanella sp. (hn-41) in batch mode and with small scale columns. a preliminary study was carried out to determine the influence of various glucose concentrations (10, 20 and 30 mm) on the bacterial growth and releasing of heavy metals from soil using a defined medium. the results showed that the heavy metals were released tremendously in the medium supplemented wi ... | 2009 | 19716584 |
| shewanella fodinae sp. nov., isolated from a coal mine and from a marine lagoon. | strains jc15(t) and jc19 were isolated from samples collected from different locations in india, including a coal mine and a marine lagoon. both strains were gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced h(2)s and showed beta-haemolysis. strain jc15(t) grew optimally at ph 6 (range ph 5-8) while strain jc19 grew optimally at ph 7 (range ph 6-9) and both had a growth temperature optimum of 30-37 degrees c (ra ... | 2010 | 19717581 |
| the arsr repressor mediates arsenite-dependent regulation of arsenate respiration and detoxification operons of shewanella sp. strain ana-3. | microbial arsenate reduction affects the fate and transport of arsenic in the environment. arsenate respiratory (arr) and detoxifying (ars) reduction pathways in shewanella sp. strain ana-3 are induced by arsenite and under anaerobic conditions. here it is shown that an arsr family protein, called arsr2, regulates the arsenate respiratory reduction pathway in response to elevated arsenite under anaerobic conditions. strains lacking arsr2 grew faster in the presence of high levels of arsenite (3 ... | 2009 | 19717602 |
| biogenic formation of as-s nanotubes by diverse shewanella strains. | shewanella sp. strain hn-41 was previously shown to produce novel, photoactive, as-s nanotubes via the reduction of as(v) and s(2)o(3)(2-) under anaerobic conditions. to determine if this ability was unique to this bacterium, 10 different shewanella strains, including shewanella sp. strain hn-41, shewanella sp. strain pv-4, shewanella alga bry, shewanella amazonensis sb2b, shewanella denitrificans os217, shewanella oneidensis mr-1, shewanella putrefaciens cn-32, s. putrefaciens ir-1, s. putrefac ... | 2009 | 19717628 |
| bacterial tracking of motile algae. | abstract we investigated whether bacterial motility and chemotaxis in the ocean enables bacteria to approach and follow microscopic, moving, point sources of nutrients. the turbulent nature of the ocean combined with the imprecision of run and tumble chemotaxis has led to the assumption that marine bacteria cannot cluster around microscopic point sources. recent work, however, shows that marine bacteria use a run and reverse strategy. we examine the ability of marine bacteria that use run and re ... | 2003 | 19719653 |
| marine bacterial organisation around point-like sources of amino acids. | abstract to better understand the trigger for and use of motility in marine bacterial chemotaxis, specific (amino acids) chemical stimuli were used to test the effect on bacterial speed and reorientation. an assay system was developed to analyse bacterial behavioural responses to point-like nutrient sources (beads 10-40 mum). the marine bacteria pseudoalteromonas haloplanktis, shewanella putrefaciens, deleya marina, the enteric bacterium escherichia coli and an enriched assemblage of marine bact ... | 2003 | 19719700 |
| in vivo electrochemistry of c-type cytochrome-mediated electron-transfer with chemical marking. | 2009 | 19722236 | |
| effect of gutting on microbial loads, sensory properties, and volatile and biogenic amine contents of european hake (merluccius merluccius var. mediterraneus) stored in ice. | the effect of gutting on sensory, microbiological, and chemical properties of european hake (merluccius merluccius var. mediterraneus) stored in ice was studied. gutting of hake noticeably affected the development of gram-negative bacteria: counts of enterobacteriaceae, shewanella putrefaciens, and pseudomonas throughout ice storage were higher in gutted than in ungutted samples. these differences in microbial loads were also reflected in the lower sensory scores of both raw and cooked hake, in ... | 2009 | 19722399 |
| biochemical and pathogenic properties of the natural isolate of shewanella algae from peter the great bay, sea of japan. | pathogenic properties of the natural isolate of shewanella algae from the coelomic fluid of the sea cucumber apostichopus japonicus (peter the great bay, sea of japan) were investigated. the isolate had oxydative metabolism, was positive for ornithine decarboxylase, cytochrome oxidase, catalase, dnase and gelatinase, hemolytically active, did not produce acid from carbohydrates, and did not hydrolyze urea and esculin. the strain was resistant to penicillin, amoxicillin, and ampicillin and suscep ... | 2009 | 19747921 |
| biosynthesis of polyunsaturated fatty acids by polyketide synthases. | 2002 | 19750617 | |
| lysine-91 of the tetraheme c-type cytochrome cyma is essential for quinone interaction and arsenate respiration in shewanella sp. strain ana-3. | the tetraheme c-type cytochrome, cyma, is essential for arsenate respiratory reduction in shewanella sp. ana-3, a model arsenate reducer. cyma is predicted to mediate electron transfer from quinols to the arsenate respiratory reductase (arrab). here, we present biochemical and physiological evidence that cyma interacts with menaquinol (mqh(2)) substrates. fluorescence quench titration with the mqh(2) analog, 2-n-heptyl-4-hydroxyquinoline-n-oxide (hoqno), was used to demonstrate quinol binding of ... | 2009 | 19760266 |
| [influence of environmental factors on electricity production by microbial fuel cell inoculation shewanella baltica]. | the influences of the anodic substance, concentration, ph and temperature on the electricity production by mfcs were discussed. the lactic sodium was better than acetic sodium or glucose. the power density of mfc and the concentration of lactic sodium were well filled with monod model. the power density was 1236 mw/m2 when the ph of anodic chamber was 8. the power density of mfc increased with the ph increasing from 6 to 8, which was due to the anodic internal resistance. the power density was 1 ... | 2009 | 19775023 |
| molecular analysis of intestinal microbiota of rainbow trout (oncorhynchus mykiss). | the aim of this study was to evaluate different molecular tools based on the 16s rrna gene, internal transcribed spacer, and the rpob gene to examine the bacterial populations present in juvenile rainbow trout intestines. dna was extracted from both pooled intestinal samples and bacterial strains. genes were pcr-amplified and analysed using both temporal temperature gradient gel electrophoresis (ttge) and restriction fragment length polymorphism methods. because of the high cultivability of the ... | 2010 | 19780831 |
| power production in mfcs inoculated with shewanella oneidensis mr-1 or mixed cultures. | power densities and oxidation-reduction potentials (orps) of mfcs containing a pure culture of shewanella oneidensis mr-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed mfc reactor configurations. the reactor architecture influenced the relative power produced by the different inocula, with the mixed culture generating 68-480% more power than mr-1 in each mfc configuration. the mixed culture produced the maximum power density of 858 +/- 9 mw ... | 2010 | 19787640 |
| electricity generation at high ionic strength in microbial fuel cell by a newly isolated shewanella marisflavi ep1. | increasing the ionic strength of the electrolyte in a microbial fuel cell (mfc) can remarkably increase power output due to the reduction of internal resistance. however, only a few bacterial strains are capable of producing electricity at a very high ionic strength. in this report, we demonstrate a newly isolated strain ep1, belonging to shewanella marisflavi based on polyphasic analysis, which could reduce fe(iii) and generate power at a high ionic strength of up to 1,488 mm (8% nacl) using la ... | 2010 | 19789866 |
| [electron shuttling function of dissolved organic matter]. | the redox reactive and electron shuttling function of dissolved organic matter (dom) derived from sewage sludge were studied with shewanella cinica d14(t) as driving force of electron transfer. the results indicated that sludge dom initially served as terminal electron acceptor obtaining electrons from the respiratory chain of quinone-reducing bacteria d14(t), then it translated to reduced dom which can donate electrons to fe(iii) again. the electron donating capacities (edc) of dom increased fr ... | 2009 | 19799291 |
| conserved synteny at the protein family level reveals genes underlying shewanella species' cold tolerance and predicts their novel phenotypes. | bacteria of the genus shewanella can thrive in different environments and demonstrate significant variability in their metabolic and ecophysiological capabilities including cold and salt tolerance. genomic characteristics underlying this variability across species are largely unknown. in this study, we address the problem by a comparison of the physiological, metabolic, and genomic characteristics of 19 sequenced shewanella species. we have employed two novel approaches based on association of a ... | 2010 | 19802638 |
| [function of phospholipids containing polyunsaturated fatty acids in cold-adapted bacteria]. | 2009 | 19803397 | |
| crystal structure of 4-hydroxybutyrate coa-transferase from clostridium aminobutyricum. | 4-hydroxybutyrate coa-transferases (4-hb-coat) takes part in the fermentation of 4-aminobutyrate to ammonia, acetate, and butyrate in anaerobic bacteria such as clostridium aminobutyricum and porphyromonas gingivalis or facultative anaerobic bacteria such as shewanella oneidensis. site-directed mutagenesis of the highly active enzyme has identified the catalytic glutamate residue as e238. crystal structure of this enzyme has been determined at a resolution of 1.85 a. the 438-amino acid residue p ... | 2009 | 19804364 |
| comparative systems biology across an evolutionary gradient within the shewanella genus. | to what extent genotypic differences translate to phenotypic variation remains a poorly understood issue of paramount importance for several cornerstone concepts of microbiology including the species definition. here, we take advantage of the completed genomic sequences, expressed proteomic profiles, and physiological studies of 10 closely related shewanella strains and species to provide quantitative insights into this issue. our analyses revealed that, despite extensive horizontal gene transfe ... | 2009 | 19805231 |
| isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from shewanella sp. br-2. | forty-four eicosapentaenoic acid (epa)-producing microbial strains were isolated from the intestines of marine fishes. among them, one strain showing a maximum level of epa (4.78%of total fatty acids) was identified as shewanella sp. br-2 on the basis of its 16s rrna sequence. the epa content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. a cosmid dna including the epa biosynthesis gene cluster consisting of pfaa ... | 2009 | 19809243 |
| a 1.5 microl microbial fuel cell for on-chip bioelectricity generation. | we have developed a dual-chamber microfluidic microbial fuel cell (mfc) system that allows on-chip bacterial culture and conversion of bacterial metabolism into electricity. the micro-mfc contains a vertically stacked 1.5 microl anode chamber and 4 microl cathode chamber, and represents the smallest mfc device to our knowledge. microfluidic deliveries of growth medium and catholyte were achieved in separate flow channels without cross-channel mass exchange. after inoculation of electrogenic shew ... | 2009 | 19823722 |
| periplasmic electron transfer via the c-type cytochromes mtra and fcca of shewanella oneidensis mr-1. | dissimilatory microbial reduction of insoluble fe(iii) oxides is a geochemically and ecologically important process which involves the transfer of cellular, respiratory electrons from the cytoplasmic membrane to insoluble, extracellular, mineral-phase electron acceptors. in this paper evidence is provided for the function of the periplasmic fumarate reductase fcca and the decaheme c-type cytochrome mtra in periplasmic electron transfer reactions in the gammaproteobacterium shewanella oneidensis. ... | 2009 | 19837833 |
| analyses of current-generating mechanisms of shewanella loihica pv-4 and shewanella oneidensis mr-1 in microbial fuel cells. | although members of the genus shewanella have common features (e.g., the presence of decaheme c-type cytochromes [c-cyts]), they are widely variable in genetic and physiological features. the present study compared the current-generating ability of s. loihica pv-4 in microbial fuel cells (mfcs) with that of well-characterized s. oneidensis mr-1 and examined the roles of c-cyts in extracellular electron transfer. we found that strains pv-4 and mr-1 exhibited notable differences in current-generat ... | 2009 | 19837834 |
| [a case of bacteremia and suppurative vertebral osteomyelitis/discitis due to shewanella algae occurring after raw-fish consumption]. | shewanella algae is an aquatic gram-negative bacterium, rarely recovered from human clinical samples. case reports of human shewanella infection are, however, slowly increasing, and a shewanella infection outbreak was reported at a south korean hospital. we report the case of an 89-year-old man admitted for back pain and fever after eating raw marine fish. sulbactam/cefoperazone was started under a tentative diagnosis of gall bladder inflammation with gallstones based on ultrasonographic finding ... | 2009 | 19860259 |
| regprecise: a database of curated genomic inferences of transcriptional regulatory interactions in prokaryotes. | the regprecise database (http://regprecise.lbl.gov) was developed for capturing, visualization and analysis of predicted transcription factor regulons in prokaryotes that were reconstructed and manually curated by utilizing the comparative genomic approach. a significant number of high-quality inferences of transcriptional regulatory interactions have been already accumulated for diverse taxonomic groups of bacteria. the reconstructed regulons include transcription factors, their cognate dna mot ... | 2010 | 19884135 |
| a polypyrrole/anthraquinone-2,6-disulphonic disodium salt (ppy/aqds)-modified anode to improve performance of microbial fuel cells. | this study reports a new approach of improving performance of microbial fuel cells (mfcs) by using a polypyrrole/anthraquinone-2,6-disulphonic disodium salt (ppy/aqds)-modified anode. the immobilization of aqds on a carbon felt anode was accomplished by electropolymerization of pyrrole while using aqds as the dopant. the dual-chamber mfc operated with this modified anode in the presence of shewanella decolorationis s12 showed the maximum power density of 1303 mw m(-2), which was 13 times larger ... | 2010 | 19889528 |
| carbon nanotubes as electrode modifier promoting direct electron transfer from shewanella oneidensis. | this study compared voltammetric behavior and catalytic current generation of shewanella oneidensis on glassy carbon electrode (gce) with and without carbon nanotube (cnt) modifier. a bare gce in an electrochemical cell inoculated with s. oneidensis delivered a low current density of 0.117+/-0.006 microa/cm(2) after being anodically polarized for 15h. cyclic voltammogram suggested current generation could be attributed to s. oneidensis's cell surface cytochromes. but the cytochromes demonstrated ... | 2010 | 19897352 |
| the mtr respiratory pathway is essential for reducing flavins and electrodes in shewanella oneidensis. | the mtr respiratory pathway of shewanella oneidensis strain mr-1 is required to effectively respire both soluble and insoluble forms of oxidized iron. flavins (riboflavin and flavin mononucleotide) recently have been shown to be excreted by mr-1 and facilitate the reduction of insoluble substrates. other shewanella species tested accumulated flavins in supernatants to an extent similar to that of mr-1, suggesting that flavin secretion is a general trait of the species. external flavins have been ... | 2010 | 19897659 |
| piezo-adapted 3-isopropylmalate dehydrogenase of the obligate piezophile shewanella benthica db21mt-2 isolated from the 11,000-m depth of the mariana trench. | 3-isopropylmalate dehydrogenase (ipmdh)-encoding leub genes were obtained from the obligate piezophile shewanella benthica db21mt-2 and non-piezophile shewanella oneidensis mr-1. the genes were expressed in escherichia coli and the proteins were purified using his-tag. the estimated kinetic parameters of these enzymes indicated that ipmdh of s. benthica db21mt-2 is more tolerant of high pressure than that of s. oneidensis mr-1. thus such an adaptation is one of the mechanisms bacteria utilize fo ... | 2009 | 19897891 |
| the cross-reactivity of shewanella fidelis lipopolysaccharide with anti-proteus antibodies. | the serological cross-reactivity between lipopolysaccharides (lps) of s. fidelis kmm3582(t) and rabbit anti-o p. mirabilis antibodies was tested. using elisa and western blot cross-reactivity between s. fidelis lps and antisera against p. mirabilis o14, o3 lpss was found. the observed cross-reaction may suggest that anti-p. mirabilis s1959 (o3) antibodies may bind to the internal part of s. fidelis o-polysaccharides. a weak interaction between s. fidelis lps and antiserum against p. mirabilis o1 ... | 2009 | 19899622 |
| microbesonline: an integrated portal for comparative and functional genomics. | since 2003, microbesonline (http://www.microbesonline.org) has been providing a community resource for comparative and functional genome analysis. the portal includes over 1000 complete genomes of bacteria, archaea and fungi and thousands of expression microarrays from diverse organisms ranging from model organisms such as escherichia coli and saccharomyces cerevisiae to environmental microbes such as desulfovibrio vulgaris and shewanella oneidensis. to assist in annotating genes and in reconstr ... | 2010 | 19906701 |
| combined effects of salting, oregano oil and vacuum-packaging on the shelf-life of refrigerated trout fillets. | the present study evaluated the effect of salt, oregano essential oil (eo) and packaging on fresh rainbow trout fillets during storage at 4 degrees c. treatments included the following: a1 (control samples, unsalted: air packaged), a2 (salted: air packaged), vp1 (salted, vacuum packaged), vp2 (salted, vacuum packaged with added oregano eo 0.2% v/wt), and vp3 salted, vacuum packaged with added oregano eo 0.4% v/wt). lactic acid bacteria (lab) (to a greater extent), followed by h(2)s-producing bac ... | 2010 | 19913701 |
| shewanella corallii sp. nov., a marine bacterium isolated from a red sea coral. | a marine bacterial strain, designated fav-2-10-05(t), was isolated from the mucus layer of a coral of the genus favia, collected from the coral reef in the gulf of eilat, israel (29.5 ° n 34.9 ° e). on the basis of 16s rrna gene sequence comparisons, strain fav-2-10-05(t) was affiliated with the family shewanellaceae. the closest relatives of strain fav-2-10-05(t) were shewanella marisflavi sw-117(t) (96.0 % 16s rrna gene sequence similarity) and shewanella haliotis dw-1(t) (95.9 %). strain fav- ... | 2010 | 19915103 |
| a structural basis for h-nox signaling in shewanella oneidensis by trapping a histidine kinase inhibitory conformation. | heme nitric oxide/oxygen (h-nox) proteins are found in eukaryotes where they are typically part of a larger protein such as soluble guanylate cyclase and in prokaryotes where they are often found in operons with a histidine kinase, suggesting that h-nox proteins serve as sensors for no and o(2) in signaling pathways. the fe(ii)-no complex of the h-nox protein from shewanella oneidensis inhibits the autophosphorylation of the operon-associated histidine kinase, whereas the ligand-free h-nox has n ... | 2009 | 19918063 |
| intracellular precipitation of pb by shewanella putrefaciens cn32 during the reductive dissolution of pb-jarosite. | jarosites (mfe(3)(so(4))(2)(oh)(6)) are precipitated in the zn industry to remove impurities during the extraction process and contain metals such as pb and ag. jarosite wastes are often confined to capped tailings ponds, thereby creating potential for anaerobic reductive dissolution by microbial populations. this study demonstrates the reductive dissolution of synthetic pb-jarosite (pbfe(6)(so(4))(4)(oh)(12)) by a subsurface dissimilatory fe reducing bacterium (shewanella putrefaciens cn32) usi ... | 2009 | 19924927 |
| structural similarities between biogenic uraninites produced by phylogenetically and metabolically diverse bacteria. | while the product of microbial uranium reduction is often reported to be "uo(2)", a comprehensive characterization including stoichiometry and unit cell determination is available for only one shewanella species. here, we compare the products of batch uranyl reduction by a collection of dissimilatory metal- and sulfate-reducing bacteria of the genera shewanella, geobacter, anaeromyxobacter, and desulfovibrio under similar laboratory conditions. our results demonstrate that u(vi) bioreduction by ... | 2009 | 19924959 |
| bacterial responses to cu-doped tio(2) nanoparticles. | the toxicity of cu-doped tio(2) nanoparticles (nps, 20nm), synthesized by a flame aerosol reactor, to mycobacterium smegmatis and shewanella oneidensis mr-1, is the primary focus of this study. both doped and non-doped tio(2) nps (20nm) tended to agglomerate in the medium solution, and therefore did not penetrate into the cell and damage cellular structures. tio(2) particles (<100mg/l) did not apparently interfere with the growth of the two species in aqueous cultures. cu-doped tio(2) nps (20mg/ ... | 2010 | 19931887 |
| novel tn4371-ice like element in ralstonia pickettii and genome mining for comparative elements. | integrative conjugative elements (ices) are important factors in the plasticity of microbial genomes. an element related to the ice tn4371 was discovered during a bioinformatic search of the ralstonia pickettii 12j genome. this element was analysed and further searches carried out for additional elements.a pcr method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight ralstonia pickettii and ralstonia insidiosa strains ... | 2009 | 19941653 |
| primary shewanella algae bacteremia mimicking vibrio septicemia. | shewanella algae infections are rare in humans. previously reported cases of s. algae have mainly been associated with direct contact with seawater. we report a case of primary s. algae bacteremia occurring after the ingestion of raw seafood in a patient with liver cirrhosis that presented a fulminent course of necrotizing fasciitis. | 2009 | 19949681 |
| investigating different mechanisms for biogenic selenite transformations: geobacter sulfurreducens, shewanella oneidensis and veillonella atypica. | the metal-reducing bacteria geobacter sulfurreducens, shewanella oneidensis and veillonella atypica, use different mechanisms to transform toxic, bioavailable sodium selenite to less toxic, non-mobile elemental selenium and then to selenide in anaerobic environments, offering the potential for in situ and ex situ bioremediation of contaminated soils, sediments, industrial effluents, and agricultural drainage waters. the products of these reductive transformations depend on both the organism invo ... | 2009 | 19950474 |
| late ventriculoperitoneal shunt infection caused by shewanella algae. | we present a case of ventriculitis and peritonitis in a child with ventriculoperitoneal shunt, which occurred 5 years after the surgery. the infection developed after contact with seawater and began as otitis. for the first time, shewanella algae, a marine microorganism, was identified as the cause of ventriculoperitoneal shunt infection. | 2010 | 19952978 |
| bioreduction of nitrobenzene, natural organic matter, and hematite by shewanella putrefaciens cn32. | we examined the reduction of nitrobenzene by shewanella putrefaciens cn32 in the presence of natural organic matter (nom) and hematite. bioreduction experiments were conducted with combinations and varied concentrations of nitrobenzene, soil humic acid, georgetown nom, hematite, and cn32. abiotic experiments were conducted to quantify nitrobenzene reduction by biogenic fe(ii) and by bioreduced noms. we show that s. putrefaciens cn32 can directly reduce nitrobenzene. both noms enhanced nitrobenze ... | 2010 | 19957913 |
| alignment of phylogenetically unambiguous indels in shewanella. | high levels of alignment errors associated with gaps have generally meant their exclusion from phylogenetic analysis. conserved inserts and deletions (indels) may in some cases be less subject to errors than amino acid substitutions for inferring the history of genomes and identifying recently laterally transferred genes, but alignment error near gaps must be evaluated prior to using indels as phylogenetic characters. a method is presented for evaluating the phylogenetic unambiguity of gaps in m ... | 2009 | 19958081 |
| comparative study of the gating motif and c-type inactivation in prokaryotic voltage-gated sodium channels. | prokaryotic voltage-gated sodium channels (na(v)s) are homotetramers and are thought to inactivate through a single mechanism, named c-type inactivation. here we report the voltage dependence and inactivation rate of the nachbac channel from bacillus halodurans, the first identified prokaryotic na(v), as well as of three new homologues cloned from bacillus licheniformis (na(v)bacl), shewanella putrefaciens (na(v)shep), and roseobacter denitrificans (na(v)rosd). we found that, although activated ... | 2010 | 19959480 |
| the periplasmic nitrate reductase in shewanella: the resolution, distribution and functional implications of two nap isoforms, napedabc and napdaghb. | in the bacterial periplasm, the reduction of nitrate to nitrite is catalysed by a periplasmic nitrate reductase (nap) system, which is a species-dependent assembly of protein subunits encoded by the nap operon. the reduction of nitrate catalysed by nap takes place in the 90 kda napa subunit, which contains a mo-bis-molybdopterin guanine dinucleotide cofactor and one [4fe-4s] iron-sulfur cluster. a review of the nap operons in the genomes of 19 strains of shewanella shows that most genomes contai ... | 2010 | 19959582 |
| rust-dissolving bugs may chow down with variety of utensils. | s. oneidensis mutant impaired in adhering to an iron oxide can still reduce it. | 2010 | 19994884 |
| aerated shewanella oneidensis in continuously fed bioelectrochemical systems for power and hydrogen production. | we studied the effects of aeration of shewanella oneidensis on potentiostatic current production, hydrogen production in a microbial electrolysis cell, and electric power generation in a microbial fuel cell (mfc). the potentiostatic performance of aerated s. oneidensis was considerably enhanced to a maximum current density of 0.45 a/m(2) or 80.3 a/m(3) (mean: 0.34 a/m(2), 57.2 a/m(3)) compared to anaerobically grown cultures. biocatalyzed hydrogen production rates with aerated s. oneidensis were ... | 2010 | 19998276 |
| role of outer-membrane cytochromes mtrc and omca in the biomineralization of ferrihydrite by shewanella oneidensis mr-1. | in an effort to improve the understanding of electron transfer mechanisms at the microbe-mineral interface, shewanella oneidensis mr-1 mutants with in-frame deletions of outer-membrane cytochromes (omcs), mtrc and omca, were characterized for the ability to reduce ferrihydrite (fh) using a suite of microscopic, spectroscopic, and biochemical techniques. analysis of purified recombinant proteins demonstrated that both cytochromes undergo rapid electron exchange with fh in vitro with mtrc displayi ... | 2010 | 20002197 |
| impact of silver(i) on the metabolism of shewanella oneidensis. | anaerobic cultures of shewanella oneidensis mr-1 reduced toxic ag(i), forming nanoparticles of elemental ag(0), as confirmed by x-ray diffraction analyses. the addition of 1 to 50 microm ag(i) had a limited impact on growth, while 100 microm ag(i) reduced both the doubling time and cell yields. at this higher ag(i) concentration transmission electron microscopy showed the accumulation of elemental silver particles within the cell, while at lower concentrations the metal was exclusively reduced a ... | 2010 | 20008067 |
| a large-scale epidemiological study to identify bacteria pathogenic to pacific oyster crassostrea gigas and correlation between virulence and metalloprotease-like activity. | a 4-year bacteriological survey (2003-2007) of four molluscs cultivated in france and faced with mortality episodes was performed by the french shellfish pathology network. the more abundant bacteria isolated during 92 mortality episodes, occurring mainly in pacific oyster crassostrea gigas, were identified by genotyping methods. it allowed us both to confirm the representativeness of vibrio splendidus and vibrio aestuarianus bacterial strains and to identify both a large number of vibrio harvey ... | 2010 | 20012275 |
| two different electron transfer pathways may involve in azoreduction in shewanella decolorationis s12. | electron transfer pathways for azoreduction by s. decolorationis s12 were studied using a mutant s12-22 which had a transposon insertion in ccma. the results imply that there are two different pathways for electron transport to azo bonds. the colony of s12-22 was whitish and incapable of producing mature c-type cytochromes whose alpha-peak was at 553 nm in the wild type s12. the mutant s12-22 could not use formate as the sole electron donor for azoreduction either in vivo or in vitro, but intact ... | 2010 | 20012540 |
| electrokinesis is a microbial behavior that requires extracellular electron transport. | we report a previously undescribed bacterial behavior termed electrokinesis. this behavior was initially observed as a dramatic increase in cell swimming speed during reduction of solid mno(2) particles by the dissimilatory metal-reducing bacterium shewanella oneidensis mr-1. the same behavioral response was observed when cells were exposed to small positive applied potentials at the working electrode of a microelectrochemical cell and could be tuned by adjusting the potential on the working ele ... | 2010 | 20018675 |
| characterization of an electron conduit between bacteria and the extracellular environment. | a number of species of gram-negative bacteria can use insoluble minerals of fe(iii) and mn(iv) as extracellular respiratory electron acceptors. in some species of shewanella, deca-heme electron transfer proteins lie at the extracellular face of the outer membrane (om), where they can interact with insoluble substrates. to reduce extracellular substrates, these redox proteins must be charged by the inner membrane/periplasmic electron transfer system. here, we present a spectro-potentiometric char ... | 2009 | 20018742 |
| enhanced reductive dechlorination of ddt in an anaerobic system of dissimilatory iron-reducing bacteria and iron oxide. | the transformation of ddt was studied in an anaerobic system of dissimilatory iron-reducing bacteria (shewanella decolorationis s12) and iron oxide (alpha-feooh). the results showed that s. decolorationis could reduce ddt into ddd, and ddt transformation rate was accelerated by the presence of alpha-feooh. ddd was observed as the primary transformation product, which was demonstrated to be transformed in the abiotic system of fe(2+)+alpha-feooh and the system of dirb+alpha-feooh. the intermediat ... | 2010 | 20031285 |
| the 285 kda bap/rtx hybrid cell surface protein (so4317) of shewanella oneidensis mr-1 is a key mediator of biofilm formation. | shewanella oneidensis, a gram-negative bacterium with unusual respiratory versatility, is found in soil and sediment environments, and sporadically as an opportunistic pathogen in humans and aquatic animals. the ability to form biofilms is a critical factor in the environmental spread and survival of this bacterium. we subjected s. oneidensis mr-1 to random transposon insertion mutagenesis to identify genes contributing to the ability of the organism to form biofilms on polystyrene surfaces. fol ... | 2010 | 20034561 |
| possible biosynthetic pathways for all cis-3,6,9,12,15,19,22, 25,28-hentriacontanonaene in bacteria. | a very long chain polyunsaturated hydrocarbon, hentriacontanonaene (c31:9), was detected in an eicosapentaenoic acid (epa)-producing marine bacterium, which was isolated from the mid-latitude seashore of hokkaido, japan, and was tentatively identified as mesophilic shewanella sp. strain osh08 from 16s rrna gene sequencing. the geometry and position of the double bonds in this compound were determined physicochemically to be all cis at positions 3, 6, 9, 12, 15, 19, 22, 25, and 28. although c31:9 ... | 2010 | 20037794 |
| development of a common oligonucleotide reference standard for microarray data normalization and comparison across different microbial communities. | high-density functional gene arrays have become a powerful tool for environmental microbial detection and characterization. however, microarray data normalization and comparison for this type of microarray remain a challenge in environmental microbiology studies because some commonly used normalization methods (e.g., genomic dna) for the study of pure cultures are not applicable. in this study, we developed a common oligonucleotide reference standard (cors) method to address this problem. a uniq ... | 2010 | 20038701 |
| hydrous manganese oxide doped gel probe sampler for measuring in situ reductive dissolution rates. 1. laboratory development. | reductive dissolution of redox-sensitive minerals such as manganese (mn) oxides in natural sediments is an important mechanism for trace element mobilization into groundwater. a gel probe sampler has been constructed to study in situ reductive dissolution of mn oxides. the gel consists of a polyacrylamide polymer matrix doped with hydrous mn oxide (hmo). gel slabs are mounted into a probe, which is designed to be inserted into the sediments. the amount of mn released from the gel by reductive di ... | 2010 | 20039731 |
| outer membrane-associated serine protease involved in adhesion of shewanella oneidensis to fe(iii) oxides. | the facultative anaerobe shewanella oneidensis mr-1 respires a variety of anaerobic electron acceptors, including insoluble fe(iii) oxides. s. oneidensis employs a number of novel strategies for respiration of insoluble fe(iii) oxides, including localization of respiratory proteins to the cell outer membrane (om). the molecular mechanism by which s. oneidensis adheres to and respires fe(iii) oxides, however, remains poorly understood. in the present study, whole cell fractionation and maldi-tof- ... | 2010 | 20039735 |
| cloning and characterization of dihydrofolate reductases from deep-sea bacteria. | enzymes from organisms living in deep-sea are thought to have characteristic pressure-adaptation mechanisms in structure and function. to better understand these mechanisms in dihydrofolate reductase (dhfr), an essential enzyme in living cells, we cloned, overexpressed and purified four new dhfrs from the deep-sea bacteria shewanella violacea (svdhfr), photobacterium profundum (ppdhfr), moritella yayanosii (mydhfr) and moritella japonica (mjdhfr), and compared their structure and function with t ... | 2010 | 20040594 |
| towards structural studies of the old yellow enzyme homologue sye4 from shewanella oneidensis and its complexes at atomic resolution. | shewanella oneidensis is an environmentally versatile gram-negative gamma-proteobacterium that is endowed with an unusually large proteome of redox proteins. of the four old yellow enzyme (oye) homologues found in s. oneidensis, sye4 is the homologue most implicated in resistance to oxidative stress. sye4 was recombinantly expressed in escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. the crystals belonged to the orthorhombic space group p2(1)2(1)2(1) an ... | 2010 | 20057079 |
| crystal structure of cold-active alkaline phosphatase from the psychrophile shewanella sp. | the crystal structure of a cold-active alkaline phosphatase from a psychrophile, shewanella sp. (scap), was solved at 2.2 a. a refined model showed a homodimer with six metal-ligand sites. the arrangement of the catalytic residues resembled those of alkaline phosphatases (aps), suggesting that the reaction mechanism of scap was fundamentally identical to those of other aps. scap had two distinct structural features: (i) a loop with arg122 that bound to the phosphate moiety of the substrate suffe ... | 2010 | 20057143 |
| [biosynthesis and function of long-chain polyunsaturated fatty acids in cold-adapted bacteria]. | 2010 | 20058713 | |
| partial degradation mechanisms of malachite green and methyl violet b by shewanella decolorationis ntou1 under anaerobic conditions. | this work demonstrated that shewanella decolorationis ntou1 decolorized 200 mg l(-1) of crystal violet, malachite green, or methyl violet b within 2-11h under anaerobic conditions at 35 degrees c. the initial color removal rate of malachite green was highest, while that of methyl violet was lowest. gc/ms analyses of the intermediate compounds produced during and after decolorization of malachite green and methyl violet b suggested that biodegradation of these dyes involved reduction to leuco for ... | 2010 | 20060225 |
| [expression and characterization of the nonspecific azoreducase of shewanella decolorationis s12]. | in order to identify the function of acpd in azoreduction, the gene was obtained from shewanella decolorationis s12 and expressed in escherichia coli top10 which showed little azoreduction activity. | 2009 | 20069878 |
| [proteins and phospholipids involved in cold-adaptation of psychrotrophic bacteria]. | 2009 | 20077850 | |
| [features of the enzymes produced by deep-sea microorganisms]. | 2009 | 20077853 | |
| the quorum-quenching n-acyl homoserine lactone acylase pvdq is an ntn-hydrolase with an unusual substrate-binding pocket. | in many gram-negative pathogens, their virulent behavior is regulated by quorum sensing, in which diffusible signals such as n-acyl homoserine lactones (ahls) act as chemical messaging compounds. enzymatic degradation of these diffusible signals by, e.g., lactonases or amidohydrolases abolishes ahl regulated virulence, a process known as quorum quenching. here we report the first crystal structure of an ahl amidohydrolase, the ahl acylase pvdq from pseudomonas aeruginosa. pvdq has a typical alph ... | 2010 | 20080736 |