| envelope glycoproteins of hantavirus can mediate cell-cell fusion independently. | hantaviruses (htvs) are enveloped viruses and can induce low ph-dependent cell fusion. in this report we molecularly cloned viral glycoproteins (gps) cdna and nucleocapsid (np) cdna of two strains of hantaan virus and one strain of seoul virus and expressed in vero e6 cells under control of a cmv promoter. the examinations of viral gene expressions were carried out by ifa and immune-precipitation. after treatment with low ph (ph 5.8) medium the syncytium were observed in the cells transfected wi ... | 2007 | 17619252 |
| a new hantaan-like virus in rodents (apodemus peninsulae) from northeastern china. | lung tissue samples of 76 korean field mice (apodemus peninsulae) collected from northeastern china bordering with far east russia and korea were detected for hantavirus partial m-segment or entire s-segment sequences by rt-pcr and 481-nt mitochondrial dna fragment of the rodents. four a. peninsulae mice were found positive for partial m-segment of hantavirus. sequence analyses of partial m-segment or/and entire s-segment of the hantaviruses revealed that three were closely related to hantaan vi ... | 2007 | 17624464 |
| [late consequences of hemorrhagic fever with renal syndrome in a woman who had it during pregnancy]. | the authors adduce a brief description of the features and outcomes of hemorrhagic fever with renal syndrome (hfrs) in 8 pregnant women. the results of the examination of a 54-year-old woman and her son, who suffered from a severe form of hers 28 years ago during the 31st week of pregnancy, are presented in detail. antibodies to hantaan virus 1:32 were found; magnetic resonance tomography of the skull revealed sequelae of hypophysial hemorrhage with the formation of "partly empty ephippium". the ... | 2007 | 17665611 |
| ribavirin reveals a lethal threshold of allowable mutation frequency for hantaan virus. | the broad spectrum of antiviral activity of ribavirin (rbv) lies in its ability to inhibit imp dehydrogenase, which lowers cellular gtp. however, rbv can act as a potent mutagen for some rna viruses. previously we have shown a lack of correlation between antiviral activity and gtp repression for hantaan virus (htnv) and evidence for rbv's ability to promote error-prone replication. to further explore the mechanism of rbv, gtp levels, specific infectivity, and/or mutation frequency was measured i ... | 2007 | 17699579 |
| detection and typing of human pathogenic hantaviruses by real-time reverse transcription-pcr and pyrosequencing. | because the clinical course of human infections with hantaviruses can vary from subclinical to fatal, rapid and reliable detection of hantaviruses is essential. to date, the diagnosis of hantavirus infection is based mainly on serologic assays, and the detection of hantaviral rna by the commonly used reverse transcription (rt)-pcr is difficult because of high sequence diversity of hantaviruses and low viral loads in clinical specimens. | 2007 | 17717126 |
| [the in vitro antiviral activity against the causative agent of hemorrhagic fever with renal syndrome]. | it is shown that primary screening of drugs against the pathogens of hemorrhagic fever with renal syndrome (hfv) may be performed, by using two approaches in estimating the suppression of plague formation and that of viral reproduction in the cultured cells. it is expedient to make a primary screening of interferon and its inductors to estimate the suppression of viral reproduction in the cultured vero e6, psev, and cl-17 cells, the infection multiplicity should be hundredth parts of boe/cell. a ... | 2007 | 17722609 |
| a soluble and heat-resistant form of hantavirus nucleocapsid protein for the serodiagnosis of hfrs. | hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne viral zoonosis characterized by fever, hemorrhagic manifestations, and renal disorder. the causative agent of hfrs has been identified as a hantavirus. hantavirus nucleocapsid proteins have recently been shown to be immunodominant antigens in hfrs, inducing an early and long-lasting immune response, and their amino termini are sensitive tools for the detection of specific antibodies in hfrs patient sera. previous work has demonstrate ... | 2008 | 17826847 |
| emergence and persistence of hantaviruses. | hantaviral diseases have been recognized for hundreds of years but, until 1976, they had not been associated with an infectious agent. when lee and colleagues isolated what is now known as hantaan virus, the techniques they introduced allowed further investigations into the etiology of the classical hantavirus disease, hemorrhagic fever with renal syndrome (hfrs), now known to be caused by any of multiple hantaviruses. the discovery of hantavirus pulmonary syndrome (hps) in the new world, and th ... | 2007 | 17848067 |
| soochong virus and amur virus might be the same entities of hantavirus. | amur virus (amrv) and soochong virus (soov) were reported to be carried by korean field mice (apodemus peninsulae) in the far east of russia, china, and korea. the distinction and demarcation between these two viruses have been a matter of debate. in order to clarify this issue and to confirm taxonomic position of amrv and soov, the extensive phylogenetic analyses based on entire s segment and entire m segment sequences of amrv, soov and other reference virus strains deposited in genbank, were c ... | 2007 | 17854038 |
| use of saccharomyces cerevisiae-expressed recombinant nucleocapsid protein to detect hantaan virus-specific immunoglobulin g (igg) and igm in oral fluid. | hantaan virus is the causative agent of severe hemorrhagic fever with renal syndrome. clinical surveillance for hantaan virus infection is unreliable, and laboratory verification is essential. the detection of virus-specific immunoglobulin m (igm) and igg in serum is most commonly used for the diagnosis of hantavirus infection. testing of oral fluid samples instead of serum offers many advantages for surveillance. however, commercial tests for hantavirus-specific antibodies are unavailable. for ... | 2007 | 17913864 |
| muju virus, a novel hantavirus harboured by the arvicolid rodent myodes regulus in korea. | acute-phase sera from >5 % of cases of haemorrhagic fever with renal syndrome occurring annually in korea have been found to exhibit a fourfold or higher antibody titre to puumala virus (puuv) than to hantaan virus (htnv) by double-sandwich igm elisa, suggesting the existence of a puuv-related hantavirus. based on the phylogenetic relationships among arvicolid rodents, the royal vole (myodes regulus) was targeted as a likely reservoir host of hantavirus. using rt-pcr, a genetically distinct hant ... | 2007 | 17947538 |
| [belgrade and hantaan hantaviruses--the causative agents of haemorrhagic fever with renal syndrome in children in serbia]. | during an outbreak of haemorrhagic fever with renal syndrome (hfrs) in 1989, five children (3 girls, 2 boys, aged 6.8-16 years) with severe clinical form of the disease, were treated at the institute of mother and child health of serbia; four of them were followed-up 22-28 months thereafter. the main clinical features were: fever, headache, myalgia, abdominal and back pains, and vomiting in all, and haemorrhagic syndrome in three; renal syndrome with severe acute renal failure in all five patien ... | 1995 | 17974469 |
| seroepidemiological study on hantavirus infections in india. | hantaviruses are etiological agents of hemorrhagic fever with renal syndrome in many parts of asia and europe. there has been no documented case of hantavirus disease from india, although serological evidence exists. we investigated the prevalence of hantavirus in the indian population and tried to identify potential risk groups for hantavirus infections. the presence of hantavirus-specific igg antibodies was prospectively evaluated in 661 subjects belonging to different groups, i.e. patients wi ... | 2008 | 17996913 |
| [the cross and unspecific reactions in serological examination for antibodies against hantavirus puumala]. | a serological survey of 78 zoologist capturing small wild rodents in their environment, 9 patients with suspected hantavirus infections and 21 patients with acute renal dysfunction for antibodies to hantaviruses was conducted in poland. survey was done by the indirect elisa with puumala and hantaan virus antigens. out of the 78 mammalogists 15 were seropositive for hantavirus puumala igg without history of clinical illness. analysis of relation between reactive zoologist's sera igg with antigens ... | 2007 | 18069385 |
| [occurrence of human hantavirus infections in poland]. | a spread of hantavirus infections causing hemorrhagic fever with renal syndrome is one of the rising epidemiological problems in the world. there is evidence that this type of viruses exists in almost all european countries. it is known that in poland all the species of wild rodents hosting european hantaviruses are present. however, the data on hantavirus are very limited and information on incidence and prevalence of hantavirus infections among humans and animals in our country is lacking. up ... | 2007 | 18069386 |
| [study on the recombinant expression of hantaan virus protein n and the establishment and application of rnp-igm direct capture elisa]. | to clone the gene encoding nucleocapsid protein (np) of hantavirus strain z10 (hv-z10), to construct its prokaryotic expression system as well as to establish a rnp-igm direct capture elisa based on hrp-labeled recombinant np (rnp), in order to detect serum samples of patients suffering from hemorrhagic fever with renal syndrome (hfrs) and to evaluate the effects of detection. | 2007 | 18069562 |
| development and evaluation of serological assays for detection of hantaanvirus-specific antibodies in human sera using yeast-expressed nucleocapsid protein. | indirect and capture enzyme-linked immunosorbent assays (elisas) for detection of hantaan virus (htnv)-specific immunoglobulins g (igg) and m (igm) in human serum samples were developed on the basis of recombinant yeast-expressed nucleocapsid (n) protein of htnv. the sensitivities and specificities of the indirect and capture elisas were evaluated by comparing the reactivity of sera from patients with hemorrhagic fever with renal syndrome (hfrs) from china with that of a commercial igg/igm kit. ... | 2008 | 18077007 |
| [identification of a functional itam-like sequence within g1 cytoplasmic tail of hantaan virus]. | the g1 cytoplasmic tail of hantaan virus (htnv) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (itam) and is termed the itam-like sequence. to demonstrate the potential signal-transducing activity of g1 itam-like sequence resembling the canonical itam within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. the synthesized g1 itam-like peptide was shown to coprecipitate ... | 2007 | 18092677 |
| interferon-stimulated genes response in endothelial cells following hantaan virus infection. | the regulation mechanism of interferon (ifn) and ifn-stimulated genes is a very complex procedure and is dependent on cell types and virus species. we observed molecular changes related to anti-viral responses in endothelial cells during hantaan virus (htnv) infection. we found that there are two patterns of gene expression, the first pattern of gene expression being characterized by early induction and short action, as in that of type i ifns,' and the other being characterized by delayed induct ... | 2007 | 18162711 |
| [transformation of full-length gene of mouse/human chimeric antibody against hantaan virus into arabidopsis thaliana]. | to construct the transgenic arabidopsis thaliana containing full-length gene of mouse/human chimeric antibody(3g1mh) against hantaan virus. | 2008 | 18177619 |
| new and old world hantaviruses differentially utilize host cytoskeletal components during their life cycles. | recently we reported that the n protein of the old world hantavirus, hantaan (htnv), traffics on microtubules to the er-golgi intermediate compartment (ergic) prior to its movement to the golgi and requires an intact ergic for viral replication. we have extended these studies to the new world hantaviruses, andes virus (andv) and black creek canal virus (bccv), and an additional old world hantavirus, seoul virus (seov). these studies support microtubule-dependent trafficking of the n protein to e ... | 2008 | 18234268 |
| landscape elements and hantaan virus-related hemorrhagic fever with renal syndrome, people's republic of china. | hemorrhagic fever with renal syndrome (hfrs) is an important public health problem in the people's republic of china, accounting for 90% of human cases reported globally. in this study, a landscape epidemiologic approach, combined with geographic information system and remote sensing techniques, was applied to increase our understanding of hfrs due to hantaan virus and its relationship with landscape elements in china. the landscape elements considered were elevation, normalized difference veget ... | 2007 | 18252099 |
| hantavirus causing hemorrhagic fever with renal syndrome enters from the apical surface and requires decay-accelerating factor (daf/cd55). | the old world hantaviruses, members of the family bunyaviridae, cause hemorrhagic fever with renal syndrome (hfrs). transmission to humans occurs via inhalation of aerosols contaminated with the excreta of infected rodents. the viral antigen is detectable in dendritic cells, macrophages, lymphocytes, and, most importantly, microvascular endothelial cells. however, the site and detailed mechanism of entry of hfrs-causing hantaviruses in polarized epithelial cells have not yet been defined. theref ... | 2008 | 18305044 |
| [development and evaluation of a double antigen sandwich elisa for the detection of total antibodies against hemorrhagic fever with renal syndrome virus]. | to develop and evaluate a method for detection of the total antibodies against hemorrhagic fever with renal syndrome (hfrs) virus with improved sensitivity and simplified operation procedure. | 2007 | 18322612 |
| [discovery, clinical and etiological characteristic of hemorrhagic fever with renal syndrome in the subtropical zone of krasnodar region]. | twenty-six patients with hemorrhagic fever with renal syndrome (hfrs) were revealed as a result of serological examination of 582 patients with fever living around sochi town. etiologic role of dobrava virus subtype as the cause of hfrs was assessed by immunofluorescent and elisa assays, and neutralization test. the principal host of this virus and source of infection for humans is caucasian forest mouse apodemus ponticus. hfrs morbidity was sporadic and not dependent from patients' occupation a ... | 2008 | 18376466 |
| synthesis of 1-beta-d-ribofuranosyl-3-ethynyl-[1,2,4]triazole and its in vitro and in vivo efficacy against hantavirus. | there are no fda approved drugs for the treatment of hemorrhagic fever with renal syndrome (hfrs), a serious human illnesses caused by hantaviruses. clinical studies using ribavirin (rbv) to treat hfrs patients suggest that it provides an improved prognosis when given early in the course of disease. given the unique antiviral activity of rbv and the lack of other lead scaffolds, we prepared a diverse series of 3-substituted 1,2,4-triazole-beta-ribosides and identified one with antiviral activity ... | 2008 | 18394724 |
| intensity of platelet beta(3) integrin in patients with hemorrhagic fever with renal syndrome and its correlation with disease severity. | beta(3) integrin has been identified as a cellular receptor for hantaan virus, which causes hemorrhagic fever with renal syndrome (hfrs). to investigate the relationship between intensity of the platelet membrane beta(3) integrin (cd61) and disease severity, the percentage of cd61-positive platelets and the mean fluorescence intensities (mfi) of platelet cd61 were determined in patients with hfrs by flow cytometry. the intensity levels of cd61 in patients with hfrs were significantly higher than ... | 2008 | 18419255 |
| genetic characterization of hantaviruses isolated from guizhou, china: evidence for spillover and reassortment in nature. | to gain more insights into the epidemiology of hantaviruses in the guizhou province, china, rodents were captured in guizhou during the period from 2001 to 2003. in addition, serum sample was collected from one patient. virus isolation was attempted from human serum and rodent samples. four hantaviruses were isolated successfully in cell culture from one human, two a. agrarius, and one r. norvegicus. the nucleotide sequences for the entire s and m and partial l segment were determined from these ... | 2008 | 18428127 |
| processing of genome 5' termini as a strategy of negative-strand rna viruses to avoid rig-i-dependent interferon induction. | innate immunity is critically dependent on the rapid production of interferon in response to intruding viruses. the intracellular pathogen recognition receptors rig-i and mda5 are essential for interferon induction by viral rnas containing 5' triphosphates or double-stranded structures, respectively. viruses with a negative-stranded rna genome are an important group of pathogens causing emerging and re-emerging diseases. we investigated the ability of genomic rnas from substantial representative ... | 2008 | 18446221 |
| real-time rt-pcr of hantaan virus rna used for the detection of virus response to antiviral drugs. | hantaan virus (htn) is an important cause of hemorrhagic fever with renal syndrome (hfrs) in korea. htn rna can be detected with the rt-pcr and the quantity of htn rna in infected cells can be measured by competitive rt-pcr. the current study used the real-time rt-pcr for the detection of viral rna s gene in a more detailed fashion than in the previous study (nam et al., virus genes 26, 31-38, 2003). a standard curve was generated with serial 10-fold dilutions of the htn rna. the sensitivity of ... | 2008 | 18459839 |
| [virological surveillance on hemorrhagic fever with renal syndrome in hunan province in 2006]. | to understand the rate of viral carrying status among rodents as well as genotypes and distribution of hantaviruses (hv) isolated in hunan province. | 2007 | 18476580 |
| heat shock protein 70 fused to or complexed with hantavirus nucleocapsid protein significantly enhances specific humoral and cellular immune responses in c57bl/6 mice. | heat shock proteins (hsps) are known to act as an effective molecular adjuvant to enhance the induction of antigen peptide-specific cellular immunity, when coupled with the antigen or peptide. hantaan virus (htnv) nucleocapsid protein (np) is relatively conserved among hantaviruses and highly immunogenic in both animals and humans. to analyze the influence of hsp70 on np vaccine potency, and evaluate the possibility of developing a novel effective vaccine against hantaviruses, we constructed pro ... | 2008 | 18479786 |
| mixing of m segment dna vaccines to hantaan virus and puumala virus reduces their immunogenicity in hamsters. | to determine if dna vaccines for two hantaviruses causing hemorrhagic fever with renal syndrome, hantaan virus and puumala virus, are immunogenic when given in combination, we delivered them to hamsters separately or as mixtures by gene gun or by electroporation. both vaccines elicited neutralizing antibodies when given alone but when they were delivered as a mixture, antibodies to only one of the two hantaviruses could be detected. in contrast, if the dnas were given as separate vaccinations to ... | 2008 | 18482782 |
| [3 cases of hemorrhagic fever with renal syndrome morbidity]. | viral origin of hemorrhagic fever with renal syndrome (hfrs) is known since 40th years of last century. also it is known that contamination of person is performed mainly by air pollution way. we observed a focus with 3 hfrs cases (with laboratory confirmation--antibodies to hfrs hantavirus were found) in one family with one lethal outcome. contamination of patients occurred by air pollution way during stocking vegetables contaminated with rodent's excrements. lethal outcome was stipulated by inc ... | 2008 | 18494292 |
| [inhibiting effects of fucoidans on hantaan virus adsorption on a model of peritoneal macrophages in vitro]. | a model of peritoneal macrophages was used to study the effect of fucoidans from brown seaweeds on the adsorption of hantaan virus. fucoidans were found to have antiviral activity, but to differ in the inhibition of hantavirus adsorption, which was associated with their structural features. the mechanism of their action is assumed to be shown via ligand-receptor interaction with certain cell membrane receptors and via blockage of hantavirus glycoproteins (g1 and g2), resulting in adsorption inhi ... | 2008 | 18590128 |
| [related substitutions in the small segment of old world hantavirus genome]. | whether there were related nucleotide substitutions in the s-segments of the genome of tula, puumala, hantaan, dorava/belgrad, and seoul viruses was studied. the significance of the correlations found in nucleotide substitutions was estimated by the level of mutual information. the majority of the correlated changes were synonymous substitutions. the correlations were mostly restricted to non-synonymous substitutions sites: tula 258 (a.a.) and dobrava/belgrad (56 a.a.). in the genome of puumala, ... | 2008 | 18590133 |
| lack of vertical transmission of hantaan virus from persistently infected dam to progeny in laboratory mice. | it is unclear how the hantaviruses are transferred from infected to uninfected rodents. we studied the status of persistently infected laboratory mice and examined the frequency of viral transmission to their offspring. expression of hantaan virus nucleocapsid protein was detected in the lungs of persistently infected dams. none of the progeny displayed viral antigen, although they were strongly positive for igg antibodies against hantavirus. there was neither hantavirus rna nor virus-specific i ... | 2008 | 18612586 |
| molecular diversity and phylogeny of hantaan virus in guizhou, china: evidence for guizhou as a radiation center of the present hantaan virus. | to gain further insight into the molecular epidemiology of hantaan virus (htnv) in guizhou, china, rodents were captured in this region in 2004 and 2005. in addition, serum samples were collected from four patients. ten hantaviruses were isolated successfully in cell culture from four humans, two apodemus agrarius, three rattus norvegicus and one rattus nitidus. the nucleotide sequences for their small (s), medium (m) and partial large (l) segments were determined. phylogenetic analysis of the s ... | 2008 | 18632971 |
| hantaan virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon, interleukin-6 and tumor necrosis factor-alpha. | hantaan virus (htnv) infects endothelial cells and is associated with increased vascular permeability during hemorrhagic fever with renal syndrome (hfrs). the pattern of increased vascular permeability is mediated by immune response. therefore, it is necessary to characterize the mechanism of htnv involvement in the host's innate immune. in this study, the expression of five toll-like receptors (tlrs) was analyzed in endothelial vein cells (evc-304) following htnv infection in vitro. tlr4 showed ... | 2008 | 18707748 |
| serum thrombospondin-1 is altered in patients with hemorrhagic fever with renal syndrome. | hemorrhagic fever with renal syndrome (hfrs) is a severe acute viral disease with pathological changes of impaired capillary and small vessels and thrombocytopenia. in this study, serum thrombospondin (tsp)-1 concentration in patients with hfrs was determined to explore its possible role in the pathogenesis of hfrs. the concentration of tsp-1 was measured using a competitive enzyme-linked immunoabsorbent assay. significantly decreased levels of serum tsp-1 were observed in hfrs patients at febri ... | 2008 | 18712840 |
| expression of a hantavirus n protein and its efficacy as antigen in immune assays. | hantavirus cardiopulmonary syndrome (hcps) has been recognized as an important public heath problem. five hantaviruses associated with hcps are currently known in brazil: juquitiba, araraquara, laguna negra-like, castelo dos sonhos, and anajatuba viruses. the laboratory diagnosis of hcps is routinely carried out by the detection of anti-hantavirus igm and/or igg antibodies. the present study describes the expression of the n protein of a hantavirus detected in the blood sample of an hcps patient ... | 2008 | 18719741 |
| situation of hantavirus infections and haemorrhagic fever with renal syndrome in european countries as of december 2006. | hantavirus infections are widely distributed in europe with the exception of the far north and the mediterranean regions. the underlying causes of varying epidemiological patterns differ among regions: in western and central europe epidemics of haemorrhagic fever with renal syndrome (hfrs) caused by hantavirus infections follow mast years with increased seed production by oak and beech trees followed by increased rodent reproduction. in the northern regions, hantavirus infections and hfrs epidem ... | 2008 | 18761927 |
| hemorrhagic fever with renal syndrome in montenegro. | the objective of the study was to analyze the epidemiological features of hemorrhagic fever with renal syndrome (hfrs) in montenegro. the study included 169 cases of hfrs diagnosed in the period between 1995 and 2005 according to the clinical symptoms and serological confirmation. for the analysis of the demographic characteristics of the cases, as well as of the chronological and topographical features of the disease, a descriptive epidemiological method was employed. the average incidence rate ... | 2008 | 18806348 |
| construction and expression of a eukaryotic expression vector containing a fusion gene of the hantaan virus s gene and hsp70 gene. | hantavirus (hv) infection leads to a kind of severe systematic syndrome, hemorrhagic fever with renal syndrome (hfrs). heat shock proteins (hsps) can be used as adjuvants assisting soluble antigens to produce specific targets which can be attacked by cytotoxic t lymphocytes. for further research on hfrs vaccine, this study aimed to express hantaan virus nucleocapsid protein (htnv np)-hsp70 fusion protein in cos-7 cells. first, an htnv s gene encoding np was amplified by pcr with a mutated termin ... | 2009 | 18810536 |
| a hantavirus causing hemorrhagic fever with renal syndrome requires gc1qr/p32 for efficient cell binding and infection. | hantaan virus (htnv) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). htnv infection is mediated by alpha v beta3 integrin. we used protein blots of vero e6 cell homogenates to demonstrate that radiolabeled htnv virions bind to gc1qr/p32, the acidic 32-kda protein known as the receptor for the globular head domain of complement c1q. rnai-mediated suppression of gc1qr/p32 markedly reduced htnv binding and infection in human lung epithelial a549 cells. conversel ... | 2008 | 18834607 |
| experience with intravenous ribavirin in the treatment of hemorrhagic fever with renal syndrome in korea. | results of a clinical study using intravenous (iv) ribavirin for treating department of defense personnel with hemorrhagic fever with renal syndrome (hfrs) acquired in korea from 1987 to 2005 were reviewed to determine the clinical course of hfrs treated with iv ribavirin. a total of 38 individuals enrolled in the study had subsequent serological confirmation of hfrs. four of the 38 individuals received three or fewer doses of ribavirin and were excluded from treatment analysis. of the remaining ... | 2009 | 18977392 |
| [participation of murine rodents in circulation of agents of tularemia and hemorrhagic fever in kola peninsula]. | results of virological and bacteriological studies of wild mammals of 11 species from rodentia and cricetidae genuses during epizootic period (spring-autumn 2006-2007) in murmansk region are presented. the number of red-baked mice (clethrionomys) and common vole (microtus) was rising. antigen of hemorrhagic fever with renal syndrome virus as well as tularemia pathogen were found in background rodent species. | 2008 | 19004288 |
| hantaan virus nucleocapsid protein binds to importin alpha proteins and inhibits tumor necrosis factor alpha-induced activation of nuclear factor kappa b. | hantaviruses such as hantaan virus (htnv) and andes virus cause two human diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, respectively. for both, disease pathogenesis is thought to be immunologically mediated and there have been numerous reports of patients with elevated levels of proinflammatory and inflammatory cytokines, including tumor necrosis factor alpha (tnf-alpha), in their sera. multiple viruses have developed evasion strategies to circumvent the host ... | 2009 | 19019947 |
| [the reactivity of sera from hemorrhagic fever in patients with renal syndromes to the recombination nucleotide proteins from european hantaviruses in hubei province]. | five yeast-expressed recombination nucleotide proteins of european hantaviruses were prepared as coated antigens to detect hantavirus-specific antibodies in sera from hemorrhagic fever with renal syndromes (hfrs) in hubei province, through elisa assay. the relativity among hantaviruses prevailing in different areas was investigated. | 2008 | 19040041 |
| antiviral effect of human saliva against hantavirus. | hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome are zoonotic diseases caused by rodent borne hantaviruses. transmission to humans occurs usually by inhalation of aerozolized virus-contaminated rodent excreta. although human-to-human transmission of andes hantavirus has been observed, the mode of transmission is currently not known. saliva from puumala hantavirus (puuv)-infected patients was shown recently to contain viral rna. to test if human saliva interferes wit ... | 2008 | 19040288 |
| [simulation of the disease caused by hantaan virus in white mice by a complex of virological, biochemical, and hematological parameters]. | the essence of studies was that the disease is simulated in 12-day albino mice subcutaneously infected with hantaan virus, strain 76-118 in a dose of 10 ld50. as an efficiency index, the study of drugs uses major (death protection coefficient, mean animal lifetime) and auxiliary (virological: pathogen accumulation in the brain tissues of deceased animals) parameters, biochemical (the levels of creatinine, urea, alanine aminotransferase, aspartate aminotransferase, malonic dialdehyde), hematologi ... | 2008 | 19069793 |
| cellular immune response to hantaan virus nucleocapsid protein in the acute phase of hemorrhagic fever with renal syndrome: correlation with disease severity. | the cellular immune response to hantaan virus (htnv) is incompletely understood, especially in humans. | 2009 | 19072554 |
| [detection of anti-hantavirus antibodies in forest workers in the north-east of poland]. | to estimate exposition to hantaviruses in a high-risk population (forest workers) in the north-east of poland. | 2008 | 19108515 |
| [application of epstein-barr virus-transformed b lymphoblastic cells in identification of ctl epitopes specific for hantaan virus]. | to establish epstein-barr virus (ebv)-transformed b lymphoblastic cell lines (b-lcl) to present peptides as antigen-presenting cells (apc) and stimulate short-cultured t cells secreting ifn-gamma, by which the t cell epitopes are identified. | 2009 | 19126382 |
| expression of l protein of hantaan virus 84fli strain and its application for recovery of minigenomes. | hantaan virus (htnv) is an old world hantavirus associated with hemorrhagic fever with renal syndrome (hfrs). to visualize the localization of the l protein of htnv strain 84fli within cells, a fusion protein composed of enhanced green fluorescent protein and l protein, egfp-l, was expressed in vero cells. the 273 kda expressed fusion protein of egfp-l localized in the perinuclear region. we also described the development of a reverse genetics system for htnv strain 84fli. the rna polymerase i ( ... | 2008 | 19133012 |
| immunological properties of a fusion protein containing nucleocapsid protein and glycoprotein gn of hantaan virus. | escherichia coli and the baculovirus bac-to-bac system were used to express chimeric gene gns0.7 consisting of glycoprotein gn gene and the 0.7 kb fragment of s genome segment carrying nucleoprotein (n) gene of hantaan virus (htnv). the expressed fusion protein gnn0.7 was recognized by monoclonal antibodies (mabs) to htnv gn glycoprotein and n protein, respectively. its molecular mass as determined by western blot analysis corresponded to the predicted value. the mice immunized with insect spodo ... | 2008 | 19143481 |
| genetic and antigenic analyses of a puumala virus isolate as a potential vaccine strain. | puumala virus (puuv), a causative agent of hemorrhagic fever with renal syndrome (hfrs), is prevalent in europe and european russia. no vaccine has been developed for puuv-associated hfrs, primarily because of the low viral yield in cultured cells. a puuv strain known as dtk/ufa-97 was isolated in russia and adapted for growth in vero e6 cells maintained in serum-free medium. the dtk/ufa-97 strain produced a higher viral titer in serum-free medium, suggesting that it may prove useful in the deve ... | 2008 | 19177744 |
| hantaviruses: an emerging public health threat in india? a review. | the emerging viral diseases haemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps) are a cause of global concern as they are increasingly reported from newer regions of the world. the hantavirus species causing hfrs include hantaan virus,seoul virus, puumala virus, and dobrava-belgrade virus while sin nombre virus was responsible for the 1993 outbreak of hcps in the four corners region of the us. humans are accidental hosts and get infected by aerosols gener ... | 2008 | 19208975 |
| hantaan virus triggers tlr3-dependent innate immune responses. | immediately after viral infection, innate responses including expression of ifn-alpha/beta and ifn-stimulated genes (isgs) are elicited ubiquitously by recruitment of specific pathogen recognition receptors. the velocity to induce ifn-alpha/beta and isgs in response to an infection is often decisive for virulence. interestingly, in primary endothelial cells isgs are induced later by hantaviruses pathogenic to humans than those considered to be nonpathogenic or of low virulence. here we demonstra ... | 2009 | 19234180 |
| acute febrile illness caused by hantavirus: serological and molecular evidence from india. | study of hantavirus infections in india is in its early stages. as early symptoms of hantavirus disease can be non-specific and the diagnosis confirmed only by laboratory testing, use of appropriate diagnostic tools is important. to improve the diagnosis of hantavirus infections in india, commercial elisa systems followed by indirect immunofluorescence assays were used to detect anti-hantavirus igm and igg in samples from patients with acute febrile illness. of 347 patients tested, 5.2% showed s ... | 2009 | 19237179 |
| [application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome]. | to establish a flow microbeads assay (fma) to examine the level of hemorrhagic fever with renal syndrome virus (hfrs v.) specific igm, igg and cytokines in hfrs patients. | 2009 | 19257990 |
| ecological surveillance of small mammals at firing points 10 and 60, gyeonggi province, republic of korea, 2001-2005. | throughout korea, small mammals are hosts to a number of disease-causing agents that pose a health threat to u.s. and korean military forces while they conduct field-training exercises. a seasonal rodent-borne disease surveillance program was established at two firing points (fp), fp-10, and fp-60, and conducted over five years from 2001 through 2005 in response to hantavirus cases among u.s. soldiers. the ecology of these sites consisted primarily of tall grasses associated with semi-permanent ... | 2008 | 19263858 |
| diagnostic aspects for epidemic hemorrhagic fever in legal medical autopsy: report of 2 cases and review. | epidemic hemorrhagic fever (ehf) is known also as hemorrhagic fever with renal syndrome (hfrs). hantaviruses, a notorious cause of ehf is spreaded by rodents or biting insects, can cause epidemic hemorrhagic fever. this report describes two rare medico-legal autopsy cases died from epidemic hemorrhagic fever. the victims were two male individuals aged 34 years and 29 years. the medico-legal autopsies were requested for alleged medical negligence. in both cases, autopsy reveals lesions to kidneys ... | 2009 | 19269214 |
| a case of graves' disease combined with hantaan virus infection. | graves' disease (gd) is generally presented by thyrotoxicosis with hyperthyroidism, and it is an organ-specific autoimmune disease induced by thyroid-stimulating hormone receptor autoantibodies. however, among diverse etiologies, viral infections have been suggested to trigger or to be involved in the pathogenesis of gd. hantaan virus infection causing hemorrhagic fever with renal syndrome (hfrs) is common in south korea and its pathogenesis is suggested to be an immunologic mechanism. we have e ... | 2009 | 19270831 |
| reduced expression of angiopoietin-1 in hantaan virus-infected human umbilical vein endothelial cell increases their permeability. | hemorrhagic fever with renal syndrome (hfrs) caused by hantaan virus (htnv) is characterized by vascular hemorrhage and acute renal failure. angiopoietin-1 (ang-1) is a glycoprotein that maintains vessel integrity and reduces endothelial permeability. we found that in htnv-infected human umbilical vein endothelial cells (huvecs) the levels of ang-1 mrna and protein were reduced on days 2 and 3 post-infection (p.i.), when endothelial permeability was increased. the htnv-stimulated permeability wa ... | 2009 | 19301945 |
| adenovirus vectors expressing hantavirus proteins protect hamsters against lethal challenge with andes virus. | hantaviruses infect humans following aerosolization from rodent feces and urine, producing hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. due to the high rates of mortality and lack of therapies, vaccines are urgently needed. nonreplicating adenovirus (ad) vectors that express andes hantavirus (andv) nucleocapsid protein (adn) or glycoproteins (adg(n) and adg(c)) were constructed. ad vectors were tested for their ability to protect syrian hamsters from a lethal andv inf ... | 2009 | 19403663 |
| low levels of serum vitronectin associated with clinical phases in patients with hemorrhagic fever with renal syndrome. | beta(3) integrin has been identified as a cellular receptor for hantaan virus which causes hemorrhagic fever with renal syndrome (hfrs). as one of the ligands of beta(3) integrin, vitronectin (vn) may be altered in hfrs. in this study, changes of serum vn levels were determined in 112 patients with hfrs and 30 age- and sex-matched healthy controls by quantitative sandwich enzyme immunoassay. the levels of serum vn were analyzed in patients at various phases of hfrs and with different severity of ... | 2009 | 19408099 |
| hantavirus pulmonary syndrome: a concise clinical review. | in 1978, hantaviruses were first described as the etiological agent of hemorrhagic fever with renal syndrome (hfrs) in korea. since then, numerous related, enveloped, negative-stranded rna viruses have been identified, forming the genus hantavirus within the family bunyaviridae. these pathogens are distributed worldwide and thus can be classified, on the basis of phylogenetic origins, into old world viruses or new world viruses (ie north, central, and south america). similarly, these viruses cau ... | 2009 | 19434035 |
| gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein. | a functionalized gold nanoparticle (gnp) enhanced ultrasensitive immuno-pcr assay (gnp-ipcr on elisa plate), which was modified from the recent developed bio-barcode assay (bca) technique, was developed to detect hantaan virus nucleocapsid protein (hnp). during the assay, the target antigen hnp was captured by a polyclonal antibody coated on elisa microplate wells, followed by adding gnp dually modified with oligonucleotides and a hnp specific monoclonal antibody l13 (mab l13) to form a sandwich ... | 2009 | 19467237 |
| genetic susceptibility to haemorrhagic fever with renal syndrome caused by hantaan virus in chinese han population. | we report a significantly higher occurrence of hla-drb1*09 (51% vs. 23%, p = 0.002, or = 3.57) and hla-b*46-drb1*09 (26% vs. 8%, p = 0.018, or = 3.76) in patients with haemorrhagic fever with renal syndrome (hfrs) compared to the control group, suggesting hantaan virus-induced hfrs is associated with a genetic predisposition in the chinese han population. | 2009 | 19473214 |
| synthesis and anti-hantaan virus activity of n(1)-3-fluorophenyl-inosine. | as part of an ongoing effort to develop new antiviral nucleoside analogs, our interest was drawn to n(1)-aryl purines as a novel structural class and potential scaffold for drug discovery. herein, we describe the synthesis of n(1)-3-fluorophenyl-inosine (fpi) and n(1)-3-fluorophenyl-hypoxanthine (fp-hx) and their antiviral activity against hantaviruses. the ec(50) for fpi and fp-hx were 94 and 234microm, respectively, against hantaan virus. fpi was not toxic to mammalian cells at concentrations ... | 2009 | 19501259 |
| hantaviruses in rodents and humans, inner mongolia autonomous region, china. | surveys were carried out in 2003-2006 to better understand the epidemiology of hantaviruses in the inner mongolia autonomous region of china (inner mongolia). hemorrhagic fever with renal syndrome (hfrs) was first reported in this region in 1955 and has been an important public health problem here since then. during 1955-2006, 8,309 persons with hfrs were reported in inner mongolia (average incidence rate 0.89/100,000), and 261 (3.14%) died. before the 1990s, all hfrs cases occurred in northeast ... | 2009 | 19523286 |
| genotyping of hantaviruses occurring in linyi, china, by nested rt-pcr combined with single-strand conformation polymorphism analysis. | the hemorrhagic fever with renal syndrome (hfrs) incidence rate still holds in high level in some areas of the world, but a reliable and simple typing method that can be used in clinical diagnosis and epidemiologic surveys is not available. in this study, 48 serum samples were collected from patients with hfrs in linyi area, china, that is seriously affected by this disease. the collected samples were analyzed by nested rt-pcr combined with single-strand conformation polymorphism (sscp) for geno ... | 2009 | 19537913 |
| [study on the difference of genes and the type identification of hantavirus from lishui, zhejiang province]. | to isolate hantavirus from lishui county--one of the epidemic regions for hemorrhagic fever with renal syndrome (hfrs), in zhejiang province, and to identify the serotype and molecular/biological characteristics of a new htn subtype hantavirus (hv) strains, hopefully to provide evidence for hfrs prevention and therapy. | 2009 | 19565882 |
| efficacy of arbidol on lethal hantaan virus infections in suckling mice and in vitro. | arbidol is an immunomodulator that was first developed in russia. in this study, we report the antiviral activity of arbidol against hantaan virus (htnv) in vitro and in vivo. | 2009 | 19575005 |
| investigating the effects of climatic variables and reservoir on the incidence of hemorrhagic fever with renal syndrome in huludao city, china: a 17-year data analysis based on structure equation model. | hfrs is a serious public health problem in china and the study on hfrs is important in china for its large population. the present study aimed to explore the impact of climatic variables and reservoir on the incidence of hfrs in huludao city, an epidemic focus of the disease in northeastern china. | 2009 | 19583875 |
| analysis of the immune response to hantaan virus nucleocapsid protein c-terminal-specific cd8(+) t cells in patients with hemorrhagic fever with renal syndrome. | hantaan virus (htnv), the prototype member of the hantavirus genus in the family bunyaviridae, causes hemorrhagic fever with renal syndrome (hfrs), which is characterized by capillary leakage, hemorrhage, and renal injury, and is an important public health problem in china. some kinds of immune cells, particularly cd8(+) t cells, are involved in the pathogenesis of hantavirus infection. the nucleocapsid protein (np) of the hantavirus is the most conserved structural protein and the most abundant ... | 2009 | 19594396 |
| increased serum fibronectin levels in patients with hemorrhagic fever with renal syndrome. | in view of the role of fibronectin in adhesion, signal transduction pathways and the infectious disease process, changes in serum fibronectin levels may influence disease evolution and severity in patients with hemorrhagic fever with renal syndrome (hfrs). | 2010 | 19701604 |
| hantavirus species in india: a retrospective study. | hantaviruses cause hemorrhagic fever with renal syndrome in europe and asia. there are about 20 documented hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. focus reduction neutralization test is the classical serotyping technique for hantavirus. however, this study employs a previously established serotyping elisa, to retrospectively analyze known hantavirus igg reactive samples for infecting serotypes. the result suggests prese ... | 2009 | 19736405 |
| the interferon response circuit in antiviral host defense. | viruses have learned to multiply in the face of a powerful innate and adaptive immune response of the host. they have evolved multiple strategies to evade the interferon (ifn) system which would otherwise limit virus growth at an early stage of infection. ifns induce the synthesis of a range of antiviral proteins which serve as cell-autonomous intrinsic restriction factors. for example, the dynamin-like mxa gtpase inhibits the multiplication of influenza and bunyaviruses (such as la crosse virus ... | 2009 | 19739399 |
| [serological characteristics of hantaviruses from clinical specimens analyzed in 1998-2008 in the department of virology, public health institute, ostrava]. | in 1998 - 2008, serum samples from 464 patients, predominantly residents of the moravia-silesian region, were examined in the department of virology, public health institute in ostrava, using enzyme-linked immunosorbent assay (elisa) for the detection of specific anti-hantavirus antibodies. seropositivity was ascertained in 41 persons (8.8%). more patients tested seropositive against hantaan virus (24, i.e. 58.5%) than against puumala virus (13, i.e. 31.7%). four persons were reactive against bo ... | 2009 | 19750821 |
| inhibition of tnf-alpha-induced activation of nf-kappab by hantavirus nucleocapsid proteins. | hantaviruses cause two human diseases thought to be immune-mediated: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). we recently reported that the nucleocapsid (n) protein of hfrs-causing hantaan virus (htnv) inhibits tumor necrosis factor-alpha (tnf-alpha) activation of nuclear factor-kappab (nf-kappab). here we measured the ability of other hantaviral n proteins to similarly interfere with the inflammatory process of tnf-alpha. we found that like htnv n, t ... | 2009 | 19751407 |
| phase i evaluation of the safety and pharmacokinetics of a single-dose intravenous injection of a murine monoclonal antibody against hantaan virus in healthy volunteers. | anti-hantaan virus monoclonal antibody (ahm) is a murine monoclonal antibody against hantaan virus being developed for the treatment of hemorrhagic fever with renal syndrome. the purpose of the present study was to describe the tolerance and pharmacokinetics of an intravenously administered single ascending dose of ahm in chinese healthy volunteers. four cohorts of 22 healthy subjects received ahm at 2.5 to 20 mg, and the results indicated that ahm was well tolerated. we established a highly sen ... | 2009 | 19752279 |
| identification of oligopeptides mimicking the receptor-binding domain of hantaan virus envelope glycoprotein from a phage-displayed peptide library. | attachment of enveloped viruses to cells is triggered by the receptor-binding domain (rbd) on envelope glycoproteins (gp) binding to receptors located on the cell surface. to date, recognized receptors and rbd of hantaan virus (htnv) have not been exactly defined. in this study, one monoclonal antibody (mab) 3g1 possessing high neutralizing activity, which is directed against htnv envelope glycoprotein g2, was used to determine the crucial motif of rbd. peptide ligands binding to mab 3g1 were se ... | 2009 | 19767836 |
| hantavirus infection in the republic of georgia. | we describe a laboratory-confirmed case of hantavirus infection in the republic of georgia. limited information is available about hantavirus infections in the caucasus, although the infection has been reported throughout europe and russia. increasing awareness and active disease surveillance contribute to our improved understanding of the geographic range of this pathogen. | 2009 | 19788822 |
| co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary. | hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ... | 2009 | 19856478 |
| hemorrhagic fever with renal syndrome in 4 us soldiers, south korea, 2005. | four us soldiers acquired hemorrhagic fever with renal syndrome while training near the demilitarized zone, south korea, in 2005. hantaan virus sequences were amplified by reverse transcription-pcr from patient serum samples and from lung tissues of striped field mice (apodemus agrarius) captured at training sites. epidemiologic investigations specified the ecology of possible sites of patient infection. | 2009 | 19891878 |
| hsp70 induced by hantavirus infection interacts with viral nucleocapsid protein and its overexpression suppresses virus infection in vero e6 cells. | hantavirus (htv) infection is known to induce innate cellular response, a more specified cellular response in the host cells. however, whether it stimulates synthesis of stress proteins, particularly associations of viral proteins, is entirely unknown. the primary focus of this research is using vero e6 cells infected with hantaan 76-118 (htnv) as an in vitro infection model to examine the individual contribution of htv infection to heat shock response. this study shows that htnv infection rapid ... | 2009 | 19956449 |
| production and characterization of a recombinant single-chain antibody against hantaan virus envelop glycoprotein. | hantaan virus (htnv) is the type of hantavirus causing hemorrhagic fever with renal syndrome, for which no specific therapeutics are available so far. cell type-specific internalizing antibodies can be used to deliver therapeutics intracellularly to target cell and thus, have potential application in anti-htnv infection. to achieve intracellular delivery of therapeutics, it is necessary to obtain antibodies that demonstrate sufficient cell type-specific binding, internalizing, and desired cellul ... | 2010 | 20012277 |
| infection of in vivo differentiated human mast cells with hantaviruses. | increased vascular permeability is a key feature of the pathological symptoms caused by hantaviruses. here, we analysed the interaction between hantaviruses and mast cells, which regulate vascular homeostasis. in highly purified human skin mast cells increasing amounts of hantaan (htnv) and, to a lower extent, prospect hill (phv) virions were produced. replication was confirmed by the production of viral plus-strand rna as determined by a virus strand-specific rt-pcr. phv but not htnv elicited e ... | 2010 | 20071485 |
| collective immunity of the population from endemic zones of hemorrhagic fever with renal syndrome in kosovo. | hemorrhagic fever with renal syndrome (hfrs), also known as mice fever is an acute viral zoonosis and it appears in the natural focus after the human contact with hantaan virus infected mice. the objective (purpose) of this study was to investigate the prevalence of specific antibodies in hfrs, in convalescent persons (collective immunity in endemic hearths). in this project we applied the epidemiological method of studying with retrospective-perspective, the serological method for determination ... | 2009 | 20088164 |
| [construction of a recombinant adenovirus carrying the hantaan virus glycoprotein g2 gene and its expression in hela cells]. | we constructed the recombinant adenovirus expressing the glycoprotein g2 of hantaan virus. firstly we obtained coding gene fragment of g2 by pcr, and subsequently inserted the gene of interest into the adenoviral pshuttle vector pad5-cmv. then we co-transfected the recombinant pshuttle vector and adenovirus skeleton plasmid into hek293 cells by calcium phosphate precipitation method. after the recombinant adenovirus were packaged and amplified in hek293 cells, we observed the expression of repor ... | 2009 | 20112705 |
| pathogenic hantaviruses direct the adherence of quiescent platelets to infected endothelial cells. | hantavirus infections are noted for their ability to infect endothelial cells, cause acute thrombocytopenia, and trigger 2 vascular-permeability-based diseases. however, hantavirus infections are not lytic, and the mechanisms by which hantaviruses cause capillary permeability and thrombocytopenia are only partially understood. the role of beta(3) integrins in hemostasis and the inactivation of beta(3) integrin receptors by pathogenic hantaviruses suggest the involvement of hantaviruses in altere ... | 2010 | 20181715 |
| [comparison on the difference of virulence between hantaan virus and seoul virus isolated both from rattus norvegicus]. | comparing the difference of virulence between the strain cgrn5310 (htnv) and the strain hr54 (seov) isolated both from rattus norvegicus. | 2009 | 20193387 |
| [complete sequence of the m segment of amur virus in rodent from china]. | to study the complete sequence of m segment of amur virus in rodents and to explore their molecular characteristics. | 2009 | 20193388 |
| [association of hemorrhagic fever with renal syndrome and hla-drb allele polymorphisms in han nationality, hubei province.]. | to understand the association of human leukocyte antigen (hla)-drb polymorphism and patients diagnosed as hemorrhagic fever with renal syndrome (hfrs). | 2009 | 20193583 |
| modulation of apoptosis and immune signaling pathways by the hantaan virus nucleocapsid protein. | herein, we show a direct relationship between the hantaan virus (htnv) nucleocapsid (n) protein and the modulation of apoptosis. we observed an increase in caspase-7 and -8, but not -9 in cells expressing htnv n protein mutants lacking amino acids 270-330. similar results were observed for the new world hantavirus, andes virus. nuclear factor kappa b (nf-kappab) was sequestered in the cytoplasm after tumor necrosis factor receptor (tnfr) stimulation in cells expressing htnv n protein. further, t ... | 2010 | 20227103 |
| hantavirus outbreak associated with laboratory rats in yunnan, china. | an outbreak of hemorrhagic fever with renal syndrome occurred among students in a college (college a) in kunming, yunnan province, china in 2003. subsequent investigations revealed the presence of hantavirus antibodies and antigens in laboratory rats at college a and two other institutions. hantavirus antibodies were detected in 15 additional individuals other than the index case in these three locations. epidemiologic data indicated that the human infections were a result of zoonotic transmissi ... | 2010 | 20380897 |
| hantavirus-like particles generated in cho cells induce specific immune responses in c57bl/6 mice. | a safe and effective hantavirus vaccine is highly desirable since hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, hfrs). virus-like particles (vlps) displaying functional viral proteins could provide effective vaccines against a few viruses, but their ability to induce hantavirus-specific immune response has not been adequately investigated. to measure the immunogenicity of hantaan virus-like particles (htn-vlps) vaccine, ... | 2010 | 20433802 |
| pathogenic hantaviruses andes virus and hantaan virus induce adherens junction disassembly by directing vascular endothelial cadherin internalization in human endothelial cells. | hantaviruses infect endothelial cells and cause 2 vascular permeability-based diseases. pathogenic hantaviruses enhance the permeability of endothelial cells in response to vascular endothelial growth factor (vegf). however, the mechanism by which hantaviruses hyperpermeabilize endothelial cells has not been defined. the paracellular permeability of endothelial cells is uniquely determined by the homophilic assembly of vascular endothelial cadherin (ve-cadherin) within adherens junctions, which ... | 2010 | 20463083 |