| association of the nucleocapsid protein of the seoul and hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules. | we performed yeast two-hybrid screening of a human kidney cell cdna library to study the biological role of the hantavirus nucleocapsid protein (np). we found that seoul virus (seov) and hantaan virus (htnv) nps were associated with small ubiquitin-like modifier (sumo)-1-interacting proteins pias1, piasxbeta, hipk2, chd3, and ttrap, which interacted with the sumo-1 conjugating enzyme (ubc-9) and sumo-1 in the yeast two-hybrid assay. interactions between the hipk2, chd3, and ttrap proteins and se ... | 2003 | 14609633 |
| [epidemic nephropathy in spain: first imported case]. | | 2003 | 14642234 |
| prevalence of rabies virus and hantaan virus infections in commensal rodents and shrews trapped in bangkok. | five hundred rodents and shrews (rattus norvegicus: 458, rattus rattus: 28, rattus exulans: 5, mus musculus: 4 and suncus murine: 5) trapped from the fresh food markets around bangkok area were investigated for rabies virus and hantaan virus infections. no rabies viral antigens in the animals' brains were detected by direct immunofluorescence. on the other hand, antibodies to hantaan virus were demonstrated in the sera of 7 (1.53%) r. norvegicus caught in various markets using a particle aggluti ... | 2003 | 14696782 |
| microarray technology for identification and distinction of hantaviruses. | dna microarrays combine high-precision technology with advanced molecular biology to achieve high-throughput screening of dna fragments. in this study, we investigated the potential of the cdna microarray technique to identify and discriminate pcr derived amplicons from genetically highly similar viruses. the wide range of sequence variation among hantaviruses makes them suitable as a model for this purpose. the hantaviruses, carried by rodents, cause several hundred thousand cases of severe hum ... | 2004 | 14981768 |
| bovine aortic endothelial cells are susceptible to hantaan virus infection. | hantavirus serotype hantaan (htn) is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs, lethality up to 10%). the natural host of htn is apodemus agrarius. recent studies have shown that domestic animals like cattle are sporadically seropositive for hantaviruses. in the present study, the susceptibility of bovine aortic endothelial cells (baec) expressing alpha(v)beta(3)-integrin to a htn infection was investigated. viral nucleocapsid protein and genomic rna segments wer ... | 2004 | 15033559 |
| [research and control of hantaan virus and hfrs]. | | 2003 | 15043085 |
| prevalence of hantavirus antibody in patients with chronic renal disease in egypt. | in egypt, the etiology of chronic renal failure (crf) is not well defined. a hospital-based case-control study was initiated in february 1998, to determine whether hantavirus infection is involved in chronic renal disease (crd) in egypt. the study enrolled 350 study patients with a history of crf and 695 matched controls with crd due to renal calculus or renal cancer, but with normal renal functions. sera from cases and controls were tested for anti-hantavirus igg using elisa with a cell-lysate ... | 2004 | 15099987 |
| a new model of hantaan virus persistence in mice: the balance between htnv infection and cd8(+) t-cell responses. | we established a viral persistence model that involves the adoptive transfer of spleen cells from immunocompetent mice (h-2(d)) into hantaan virus (htnv)-infected severe combined immunodeficient (scid, h-2(d)) mice. the infection is maintained despite the presence of neutralizing antibodies, without apparent signs of disease, and there is a correlation between htnv persistence and the lack of htnv-specific cd8(+) t cells. in addition, disseminated htnv infection before the initiation of immune r ... | 2004 | 15110529 |
| analysis of n-linked glycosylation of hantaan virus glycoproteins and the role of oligosaccharide side chains in protein folding and intracellular trafficking. | the membrane glycoproteins gn and gc of hantaan virus (htnv) (family bunyaviridae) are modified by n-linked glycosylation. the glycoproteins contain six potential sites for the attachment of n-linked oligosaccharides, five sites on gn and one on gc. the properties of the n-linked oligosaccharide chains were analyzed by treatment with endoglycosidase h, peptide:n-glycosidase f, tunicamycin, and deoxynojirimycin and were confirmed to be completely of the high-mannose type. ten glycoprotein gene mu ... | 2004 | 15113920 |
| [expression of amino terminal of nucleoprotein and glycoprotein g2 of hantaan virus in insect cells in the form of fusion protein]. | to express the glycoprotein g2 and amino terminal of nucleoprotein (np) of hantaan virus in bac-to-bac baculovirus expression system in the form of fusion protein. | 2003 | 15155073 |
| differential antiviral response of endothelial cells after infection with pathogenic and nonpathogenic hantaviruses. | hantaviruses represent important human pathogens and can induce hemorrhagic fever with renal syndrome (hfrs), which is characterized by endothelial dysfunction. both pathogenic and nonpathogenic hantaviruses replicate without causing any apparent cytopathic effect, suggesting that immunopathological mechanisms play an important role in pathogenesis. we compared the antiviral responses triggered by hantaan virus (htnv), a pathogenic hantavirus associated with hfrs, and tula virus (tulv), a rather ... | 2004 | 15163707 |
| [screening and identification of mimic epitopes of monoclonal antibodies against hantaan virus using phage display technique]. | to obtain mimic epitopes of monoclonal antibody (mab) against hantaan virus, and identify preliminarily their immunological characteristics. | 2004 | 15207087 |
| identification and analysis for cross-reactivity among hantaviruses of h-2b-restricted cytotoxic t-lymphocyte epitopes in sin nombre virus nucleocapsid protein. | sin nombre virus (snv) causes hantavirus pulmonary syndrome (hps), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. in humans, cytotoxic t lymphocytes (ctl) specific for snv appear to play an important role in the pathogenicity of hps. there is a correlation between the frequencies of snv-specific ctls and the severity of hps disease. in order to create a mouse model to study the role of snv-specific t cells in vivo, t cell respo ... | 2004 | 15218176 |
| modification of endothelial cell functions by hantaan virus infection: prolonged hyper-permeability induced by tnf-alpha of hantaan virus-infected endothelial cell monolayers. | serious vascular leakage is central to the pathogenesis of hantavirus infections. however, there is no evidence suggesting the hantavirus infection of endothelial cells directly causes obvious cell damage or morphological alteration either in vivo or in vitro. in this study, we examined whether hantaan virus (htnv) infection modifies the barrier function of endothelial cell monolayers upon the exposure to pro-inflammatory cytokines. low levels (1 ng/ml) of tumor necrosis factor-alpha initially i ... | 2004 | 15221531 |
| age-dependent hantavirus-specific cd8(+) t-cell responses in mice infected with hantaan virus. | to investigate age-dependent differences in hantavirus-specific cd8(+) t-cell responses, mice were inoculated with 0.1 50% newborn mouse lethal dose of hantaan virus (htnv) at 0, 3, 7, 14, or 35 days after birth. htnv-specific cd8(+) t cells producing gamma interferon (ifn-gamma) were measured on day 30 after htnv inoculation. although no ifn-gamma-producing htnv-specific cd8(+) t cells were detected in most of the mice inoculated with htnv on day 0 after birth, most mice inoculated at 3, 7, 14, ... | 2004 | 15221537 |
| serological characterization of a hantavirus from hubei, china. | hantavirus hv114, isolated from urine of a patient during epidemic of hemorrhagic fever with renal syndrome (hfrs) in china, was subjected to a detailed serological characterization using enzyme-linked immunosorbent assay (elisa), neutralization test and indirect immunofluorescence antibody assay (ifa). it has been found that hv114 is antigenically similar to the hantavirus a9 strain isolated in china and to the hantaan 76-118 virus (htnv 76-118), but different from the hantaviruses isolated fro ... | 2004 | 15230468 |
| [the epidemic characteristics and preventive measures of hemorrhagic fever with syndromes in china]. | to summarize and analyze the epidemic situation of hantaviruses including geographic distribution, types and prevalent intensity of epidemic areas of hantavirus for the last 30 years in china, and to discuss relative preventive measures. | 2004 | 15231118 |
| [serological surveillance on vaccine against hemorrhagic fever with renal syndrome]. | to observe the serological and epidemiological efficacy of hemorrhagic fever renal syndrome (hfrs) vaccine in zhejiang province. | 2004 | 15231163 |
| [hemorrhagic fever with renal syndrome: historical aspects]. | a mysterious disease was first reported from korea when it had been observed during late spring 1951 in un forces operating in the central area close to the 38th parallel. the disease showed distinctive features which included high fever, low blood pressure, hemorrhagic tendency and acute renal failure. historically it was apparently a similar disease to a clinical entity designated as epidemic hemorrhagic fever in manchuria or hemorrhagic nephrosonephritis in far eastern russia. after lee ho-wa ... | 2004 | 15309770 |
| [a study on detecting specific antibodies of hemorrhagic fever with renal syndrome and treatment with integrated traditional chinese and western medicine]. | to explore a simple speedy specific and sensitive method to detect specific igm (sigm) and igg (sigg) antibodies of hemorrhagic fever with renal syndrome (hfrs),and to study the therapeutic effects of integrated traditional chinese and western medicine on hfrs. | 2004 | 15339496 |
| [cellular ultrastructural changes of bone marrow of patients with hemorrhagic fever with renal syndrome]. | to observe cytopathogenic effect of hantaan virus (hv) on cultured human bone marrow cells. | 2004 | 15340508 |
| [experimental study on enzyme dot assay for detection of hemorrhagic fever with renal syndrome antigen]. | to establish a new and efficient method(ieda) for detection of hemorrhagic fever with renal syndrome virus (hfrsv) antigen. | 2004 | 15340537 |
| [using recombinant antigens of hantavirus to study the kinetics of serum iga, igg, igm antibodies in the acute-phase of hemorrhagic fever renal syndrome]. | to observe the features of serum specific iga, igg, igm antibodies in the acute phase of hemorrhagic fever renal syndrome (hfrs). | 2003 | 15340571 |
| cell fusion activities of hantaan virus envelope glycoproteins. | hantaan virus (htnv)-infected vero e6 cells undergo cell fusion with both infected and uninfected cells under low-ph conditions. flow cytometry and fluorescence microscopy of htnv-infected vero e6 cells showed that envelope glycoproteins (gps) were located both on the cell surface and in the cytoplasm. neutralizing monoclonal antibodies (mabs) against the g1 and g2 envelope gps inhibited cell fusion, whereas nonneutralizing mabs against g1 or g2 and mabs against the nucleocapsid protein (np) did ... | 2004 | 15367644 |
| [amplification test-systems in the diagnosis of hemorrhagic fever with renal syndrome and in studies of viral pathogens]. | the "vectohantivirus-ampli" test system based on rtpcr was shown as fitting the detection of virus rna in blood samples of patients with hemorrhagic fever with renal syndrome (hfrs) made at early stages and no later than 7 days from the disease onset. a sequence analysis of viral nucleotide sequences of pcr products produced by the test-system ensured the identification of hantaviruses (pathogens of hfrs). two genetic variants of puumula virus were shown to circulate in the territory of the bash ... | 2004 | 15455692 |
| the discovery of hantaan virus: comparative biology and serendipity in a world at war. | | 2004 | 15478081 |
| [progress in the epidemiologic study of hemorrhagic fever with renal syndrome in china in recent years]. | | 2003 | 15481132 |
| isolation of the etiologic agent of korean hemorrhagic fever. 1978. | | 2004 | 15517702 |
| [prevalence of hemorrhagic fever with renal syndrome virus in domestic pigs: an epidemiological investigation in shandong province]. | to investigate the epidemiological significance of hemorrhagic fever with renal syndrome virus (hfrsv) infection in domestic pigs in shandong province, and study the role of domestic pigs in the prevalence of hfrs. | 2004 | 15567780 |
| [preliminary studies on proliferation of hemorrhagic fever with renal syndrome virus in leptotrombidium (l.) scutellare]. | in order to observe further the proliferation and kinetic changes of hemorrhagic fever with renal syndrome virus (hfrsv) in leptotrombidium (l.) scutellare infected with hfrsv, the raised leptotrombidium (l.) scutellare which were at larve stage or at nymph stage were grinded and sterilized by filtration at interval of 20 days, each batch of the filtrate was inoculated separately to vero-e6 cells and the titre (tcid50/ml) of hfrsv was measured. the results confirmed: hfrsv were isolated from eac ... | 1997 | 15617343 |
| [purification of hemagglutinin of hemorrhagic fever with renal syndrome virus by high pressure liquid chromatography and analysis of immunogenicity]. | the brains of suckling mice and suspension of vero-e6 cells inoculated with chen's strain of hemorrhagic fever with renal syndrome virus (hfrsv) were obtained and prepared into high titre hemagglutinin (han). the crude han was purified by high pressure liquid chromatography. the molecular weight of the purified han is 56600 and has higher titre of hemagglutinin activity. it has been proved that the purified han has homogeneous molecular weight and buoyant density through sds-page and sucrose gra ... | 1997 | 15619826 |
| [generation of human monoclonal antibodies against hemorrhagic fever with renal sydrome (hfrs) virus]. | peripheral blood lymphocytes (pbl) were obtained from healthy donors and treated with l-leucy-leucin methy (leuleu-ome). the leuleu-ome treated pbl were in vitro immunized with hfrs virus proteins (55kd, 67kd) for 6 days, and then fused with k6h6/b5 cells in medium containing 45% (peg) and 7.5% dmso. the fused cells were seeded in 96-well plates at cell density 0.5-1 x 10(5) cells/well in hat supplemented rpmi-1640 medium. after 14-20 days, 3 positive wells were deteced by elisa and two (1b7, 2d ... | 1997 | 15619910 |
| interactions and trafficking of andes and sin nombre hantavirus glycoproteins g1 and g2. | this study was designed to investigate the trafficking of andes virus (andv) and sin nombre virus (snv) glycoproteins and to determine if andv or snv glycoproteins g1 and g2 could be substituted for each other while still retaining normal trafficking. trafficking of hantaan virus (hntv) and snv glycoproteins has been studied and conflicting results were published regarding the golgi targeting of g1 and g2 when expressed individually. the results reported in this manuscript suggest that both snv ... | 2005 | 15629773 |
| [the relationship between cellular infection by hantaan virus and expression of beta3 integrin]. | to investigate the relationship between cellular entry of hantaan virus (htnv) and expression of beta3 integrin in beta3-integrin-deficient and htnv-insusceptible china hamster ovary (cho) cells. | 2004 | 15634536 |
| [complex evaluation of endemicity level of the territory affected by hemorrhagic fever with renal syndorome]. | a new formula for the calculation of the endemicity index of the territory affected by hemorrhagic fever with renal syndrome (hfrs) based on empiric data collected in the volga region near saratov in 1982-2000 is proposed. as found in this research 21% of the administrative districts of the region have a high endemicity level (with the average index for several years reaching 35 points), 8% of the districts have a medium enemicity level (20 points) and 71% of the districts--a low endemicity leve ... | 2004 | 15636134 |
| pathogenic hantaviruses bind plexin-semaphorin-integrin domains present at the apex of inactive, bent alphavbeta3 integrin conformers. | the alphavbeta3 integrins are linked to human bleeding disorders, and pathogenic hantaviruses regulate the function of alphavbeta3 integrins and cause acute vascular diseases. alphavbeta3 integrins are present in either extended (active) or dramatically bent (inactive) structures, and interconversion of alphavbeta3 conformers dynamically regulates integrin functions. here, we show that hantaviruses bind human alphavbeta3 integrins and that binding maps to the plexin-semaphorin-integrin (psi) dom ... | 2005 | 15657120 |
| identification of dobrava, hantaan, seoul, and puumala viruses by one-step real-time rt-pcr. | we developed four assays for specifically identifying dobrava (dob), hantaan (htn), puumala (puu), and seoul (seo) viruses. the assays are based on the real-time one-step reverse transcriptase polymerase chain reaction (rt-pcr) with the small segment used as the target sequence. the detection limits of dob, htn, puu, and seo assays were 25, 25, 25, and 12.5 plaque-forming units, respectively. the assays were evaluated in blinded experiments, each with 100 samples that contained andes, black cree ... | 2004 | 15664046 |
| recombinant tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture. | tula hantavirus carrying recombinant s rna segment (rectulv) grew in a cell culture to the same titers as the original cell adapted variant but presented no real match to the parental virus. our data showed that the lower competitiveness of rectulv could not be increased by pre-passaging in the cell culture. nevertheless, the recombinant virus was able to survive in the presence of the parental virus during five consecutive passages. the observed survival time seems to be sufficient for transmis ... | 2005 | 15725355 |
| nucleocapsid protein of cell culture-adapted seoul virus strain 80-39: analysis of its encoding sequence, expression in yeast and immuno-reactivity. | seoul virus (seov) is a hantavirus causing a mild to moderate form of hemorrhagic fever with renal syndrome that is distributed mainly in asia. the nucleocapsid (n) protein-encoding sequence of seov (strain 80-39) was rt-pcr-amplified and cloned into a yeast expression vector containing a galactose-inducible promoter. a survey of the pattern of synonymous codon preferences for a total of 22 n protein-encoding hantavirus genes including 13 of seov strains revealed that there is minor variation in ... | 2005 | 15744561 |
| [development of purified hfrs bivalent vaccine]. | to develop the purified hfrs bivalent vaccine. | 2005 | 15766416 |
| [using geographic information system to study on the association between epidemic areas and main animal hosts of hemorrhagic fever with renal syndrome in china]. | to analyze the association between types of epidemic areas and proportion of main animal hosts of hemorrhagic fever with renal syndrome (hfrs) in china. | 2004 | 15769318 |
| [epidemiological study of hemorrhagic fever with renal syndrome in 1984-2003 in weifang city, shandong province]. | | 2004 | 15769383 |
| human and rodent humoral immune responses to andes virus structural proteins. | in the present work we identified b-cell epitopes recognized by sera of humans and rodents naturally infected with andes virus, a hantavirus present in chile and argentina. analysis of patient and rodent sera with overlapping peptides revealed 21 human and rodent epitopes on the three structural proteins. whereas in the nucleoprotein the region comprising aa 248-260 was shown to be the key determinant of human sera, the major antigenic site of rodent antibody reactivity is located at aa 326-338. ... | 2005 | 15780882 |
| detection of human hantavirus infections in lithuania. | in europe certain hantaviruses are known to cause hemorrhagic fever with renal syndrome of different severity. the objective of the present investigation was to study the presence of hantavirus infections in lithuania. | 2005 | 15827873 |
| in vivo characterization of the integrin beta3 as a receptor for hantaan virus cellular entry. | binding of viruses to cell surface molecules is an essential step in viral infection. in vitro studies suggested that the alpha(v)beta(3) integrin receptor is the epithelial cell receptor for hantaan virus (htnv). whether beta(3) is in vivo the only or central cellular receptor for htnv infection is not known. to investigate the role of beta(3) integrin for cellular entry of htnv, we established an htnv infection model in newborn murine pups. infected pups died at an average age of 14.2 +/- 1.1 ... | 2005 | 15886525 |
| hypopituitarism as a late complication of hemorrhagic fever. | we report three patients who developed hypopituitarism as a late complication of hemorrhagic fever with renal syndrome (hfrs). their past history, physical examination, and endocrine investigation confirmed hypopituitarism. magnetic resonance imaging of the pituitary revealed atrophic pituitary gland with an empty sella. hemorrhagic fever is endemic in certain regions of the balkans, and this preliminary report suggests the importance of investigating the endocrine status in every patient who su ... | 2005 | 15888918 |
| immunization effect of purified bivalent vaccine to haemorrhagic fever with renal syndrome manufactured from primary cultured hamster kidney cells. | | 2005 | 15899141 |
| hantavirus infection in thailand: first clinical case report. | this study involved 115 cases of fever of unknown origin (fuo) in patients who were admitted to the department of medicine, siriraj hospital from may 1999 to november 2000. among the patient sera screened by elisa for igg hantavirus, five were positive for igg hantavirus-reacting antibodies and eight tested positive for igm hantavirus-reacting antibodies. one serum had both igg and igm antibodies. the patient exhibited acute encephalitic febrile illness, thrombocytopenia, high ast and alt levels ... | 2005 | 15906672 |
| long-term sequelae of hemorrhagic fever with renal syndrome attributable to hantaan virus in korean war veterans. | health status was sought for approximately 1600 korean war veterans who contracted hemorrhagic fever with renal syndrome (hfrs) during deployment to korea between 1951 and 1953. to determine whether long-term sequelae were present for these individuals, mortality and morbidity data were collected from the department of veterans affairs, the centers for medicare and medicaid services, the social security administration, and the national death index records. control subjects were selected from mil ... | 2005 | 15916302 |
| peptide antagonists that inhibit sin nombre virus and hantaan virus entry through the beta3-integrin receptor. | specific therapy is not available for the treatment of hantavirus cardiopulmonary syndrome caused by sin nombre virus (snv). the entry of pathogenic hantaviruses into susceptible human cells is dependent upon expression of the alpha(v)beta(3) integrin, and transfection of human beta(3) integrin is sufficient to confer infectibility onto cho (chinese hamster ovary) cells. furthermore, pretreatment of susceptible cells with anti-beta(3) antibodies such as c7e3 or its fab fragment reopro prevents h ... | 2005 | 15919886 |
| inactivation of hantaan virus-containing samples for subsequent investigations outside biosafety level 3 facilities. | the potential risk of accidental infection by hantaviruses in a clinical or research laboratory necessitates special precautionary measures. a biosafety program must address handling and disposal of infectious materials as well as appropriate virus inactivation or depletion procedures to permit necessary further processing of specimens outside the biosafety level 3 laboratory. | 2005 | 15920350 |
| variability of g1 gene of hantaviruses occurring in the hubei province, p.r. china from 1985 to 2000. | we studied variability of g1 gene of hantaviruses occurring in the hubei province, p.r. china. serum samples were collected from 229 patients with hemorrhagic fever with renal syndromes (hfrs) during 1985--1989 and 1996--2000 and were tested by rt-pcr for the presence of hantaan and seoul viruses (htnvs, seovs) and by restriction fragment length polymorphism (rflp) analysis for the respective pattern. out of 229 sera 166 (72.5%) were hantavirus-positive by rt-pcr, including 124 from 1985--1989 a ... | 2005 | 15929399 |
| hantavirus nucleocapsid protein: a multifunctional molecule with both housekeeping and ambassadorial duties. | in recent years important progress has been made studying the nucleocapsid (n) protein of hantaviruses. the n protein presents a good example of a multifunctional viral macromolecule. it is a major structural component of a virion that encapsidates viral rna (vrna). it also interacts with the virus polymerase (l protein) and one of the glycoproteins. on top of these "house keeping" duties, the n protein performs interactive "ambassadorial" functions interfering with important regulatory pathways ... | 2005 | 15931462 |
| hanta hemorrhagic fever with renal syndrome: a case report and review. | hantavirus infection is seldom reported in taiwan. the spectrum of clinical severity ranges from mild to severe and may cover hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome (hfrs). we report a case of hfrs in a 24-year-old soldier presenting with fever, chills, abdominal pain and generalized myalgia. his clinical course progressed through febrile, hypotensive, oliguria and polyuria phases. hantavirus infection was proven by serology at the second hospital week. the patie ... | 2005 | 15986074 |
| [expression and genetic immunization of hantaan virus g2 recombinant adenovirus]. | to express hantaan virus(htnv) envelope glycoprotein g(2) recombinant adenovirus(adeno-g(2)) in vero e6 cells and explore its property of inducing immune response. | 2005 | 15989780 |
| essential amino acids of the hantaan virus n protein in its interaction with rna. | the nucleocapsid (n) protein of hantavirus encapsidates viral genomic and antigenomic rnas. previously, deletion mapping identified a central, conserved region (amino acids 175 to 217) within the hantaan virus (htnv) n protein that interacts with a high affinity with these viral rnas (vrnas). to further define the boundaries of the rna binding domain (rbd), several peptides were synthesized and examined for the ability to bind full-length s-segment vrna. peptide 195-217 retained 94% of the vrna ... | 2005 | 16014963 |
| hantavirus infection in thailand: first clinical case report. | this study involved 115 cases of fever of unknown origin (fuo) patients who were admitted to the department of medicine, siriraj hospital from may 1999 to november 2000. among the patient sera screened by elisa for igg hantavirus, five were positive for igg hantavirus-reacting antibodies and eight tested positive for igm hantavirus-reacting antibodies. one serum had both igg and igm antibodies. the patient exhibited acute encephalitic febrile illness, thrombocytopenia, high ast and alt levels, a ... | 2005 | 16124441 |
| immunogenicity of combination dna vaccines for rift valley fever virus, tick-borne encephalitis virus, hantaan virus, and crimean congo hemorrhagic fever virus. | dna vaccines for rift valley fever virus (rvfv), crimean congo hemorrhagic fever virus (cchfv), tick-borne encephalitis virus (tbev), and hantaan virus (htnv), were tested in mice alone or in various combinations. the bunyavirus vaccines (rvfv, cchfv, and htnv) expressed gn and gc genes, and the flavivirus vaccine (tbev) expressed the prem and e genes. all vaccines were delivered by gene gun. the tbev dna vaccine and the rvfv dna vaccine elicited similar levels of antibodies and protected mice f ... | 2006 | 16174542 |
| effect of cordycepin on hantaan virus 76-118 infection of primary human embryonic pulmonary fibroblasts--characterization of apoptotic effects. | the cdna microarray technique was used to study gene epression in human embryonic pulmonary fibroblasts (hepf) infected with hantaan virus (htnv) under the influence of cordycepin (cor), an inhibitor of post-transcriptional pre-mrna polyadenylation. four apoptotic genes, the insulin-like growth factor binding protein 1, nfkb inhibitor alpha, caspase-3 and nfkb1 were up-regulated in both infected and uninfected cor-treated cells and two cell cycle-associated genes, cdc-like kinase and beta-induce ... | 2005 | 16178516 |
| design and development of a dna array for rapid detection and genotyping of seven kinds of pathogenic microbes. | a dna array for rapid detection and genotyping of the pathogenic microbes of epidemic hemorrhagic fever, tsutsugamushi disease, leptospirosis, malaria, schistosomiasis, cholera, and hemorrhagic colitis was developed. the specific and relatively conserved pcr primers and dna probes were screened from the characteristic genes of the pathogenic microbes. the pcr or rt-pcr methods were established for amplifying and labeling the dna fragments of the pathogenic microbes. all the probes with the same ... | 2005 | 16193981 |
| [identification of oligopeptides mimicking the virus attachment protein of hantaan virus]. | to identify and characterize the epitope associated with the virus attachment protein (vap) of hantaan virus. | 2005 | 16201476 |
| [identification of htnv-np-specific t lymphocyte epitopes and analysis of the epitope-specific t cell response]. | to identify the nucleocapsid protein of hantaan virus (htnv-np)-specific t lymphocyte epitopes and analyze the epitope-specific t cell response during hemorrhagic fever with renal syndrome (hfrs). | 2005 | 16256031 |
| [hemorrhagic fever with renal syndrome among servicemen in primorskiĭ region of russia]. | the data about the incidence of hemorrhagic fever with renal syndrome (hfrs) among the servicemen stationed in hfrs endemic territories at primorye region are presented. the authors have shown the epidemiological significance of outbreaks, group and sporadic cases of hfrs especially in servicemen aged 18-20. during the outbreaks the maximal hfrs incidence is registered during 2 weeks after the first case. 104 hfrs cases were registered in the servicemen. among them 77.8 +/- 3.0% were at the age ... | 2005 | 16276769 |
| cellular entry of hantaan virus a9 strain: specific interactions with beta3 integrins and a novel 70kda protein. | cellular entry of pathogenic hantaviruses had been shown to be mediated by beta3 integrins. however, no direct evidence exists that hantavirus binds to beta3 integrins, and integrin beta3 subunit is not expressed on some cells permissive to hantavirus infection. in this report, utilizing beta3-integrin-transfected cho cells, we demonstrated that integrin beta3 subunit renders cho cells susceptible to chinese hantaan virus (htn) strain a9 (isolated in china), and the viral infection was correspon ... | 2006 | 16310165 |
| [changing patterns of communicable diseases in korea]. | before twentieth centuries and during early twentieth centuries, communicable diseases were the major cause of morbidity and mortality in korea. but reliable data are not available. after 1975, the overall morbidity and mortality from communicable diseases, rapidly declined. recently many new pathogenic microbes were recognized: l. monocytogenes, hantaan virus, y. pseudotuberculosis, p. multocida, l. pneumophilia, human immunodeficiency virus (hiv), g. seoi, h. capsulatum, c. burnetii, v. choler ... | 2005 | 16315747 |
| hantaan/andes virus dna vaccine elicits a broadly cross-reactive neutralizing antibody response in nonhuman primates. | at least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (hfrs); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (hps). the most prevalent and lethal hantaviruses associated with hfrs and hps are hantaan virus (htnv) and andes virus (andv), respectively. here, we constructed a dna vaccine plasmid (pwrg/ha-m) that contains both the htnv and andv m gene segments. rhesus ma ... | 2006 | 16378630 |
| [antiviral effectiveness of the combined use of amixine and virasole in experimental hemorrhagic fever with renal syndrome in sucking albino mice]. | the antiviral effectiveness of the combined and single use of superlow-dose amixine and virasole on the course of experimental hemorrhagic fever with renal syndrome was studied in sucking albino mice parenterally infected with their virus hantaan. the co-administration of virasole and amixine was shown to protect 52% of the infected animals from death, which is superior to the effect of their monotherapy. the combined use of the drugs substantially prolongs the survival of albino mice after thei ... | 2005 | 16408628 |
| analysis of immune responses against nucleocapsid protein of the hantaan virus elicited by virus infection or dna vaccination. | even though neutralizing antibodies against the hantaan virus (htnv) has been proven to be critical against viral infections, the cellular immune responses to htnv are also assumed to be important for viral clearance. in this report, we have examined the cellular and humoral immune responses against the htnv nucleocapsid protein (np) elicited by virus infection or dna vaccination. to examine the cellular immune response against htnv np, we used h-2k(b) restricted t-cell epitopes of np. the np-sp ... | 2005 | 16410771 |
| a pseudotype vesicular stomatitis virus containing hantaan virus envelope glycoproteins g1 and g2 as an alternative to hantavirus vaccine in mice. | we examined whether a vesicular stomatitis virus (vsv) pseudotype bearing the hantavirus envelope glycoproteins (gps) g1 and g2 (vsvdeltag*htn) could be used as a safe and effective alternative to native hantavirus. mice were immunized with purified particles of vsvdeltag*htn. after the second immunization, all mice produced anti-gp antibody as detected in elisa and a neutralization test. after the third immunization, the mice were challenged with hantaan virus. neither anti-np antibody producti ... | 2006 | 16426712 |
| [serological screening in the establishment of the epidemic activity of foci of hemorrhagic fever with renal syndrome]. | | 2005 | 16498958 |
| dobrava, but not saaremaa, hantavirus is lethal and induces nitric oxide production in suckling mice. | hantaviruses are the causative agents of hfrs and hcps (hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome), two severe, and often fatal human diseases. mortality from hfrs varies between hantaviruses; hantaan and dobrava show the highest, seoul intermediate, and puumala low mortality. saaremaa, genetically closely related to dobrava, is also known to induce hfrs, with low or no mortality. in this study, mice were inoculated with dobrava and saaremaa viruses to test fo ... | 2006 | 16513381 |
| dynamic changes of apoptosis-inducing ligands and th1/th2 like subpopulations in hantaan virus-induced hemorrhagic fever with renal syndrome. | the expression of the apoptosis-inducing ligands, tnf-alpha, fasl and trail on peripheral blood mononuclear cells (pbmc) and the levels of their soluble form (tnf-alpha, sfasl and strail) in plasma from 40 hemorrhagic fever with renal syndrome (hfrs) patients as well as 26 healthy blood donors were determined by flow cytometry (fcm) analysis and sandwich elisa, respectively. the status of th1, th2, tc1 and tc2 subsets in pbmc was evaluated by intracellular cytokine staining and fcm. compared to ... | 2006 | 16616877 |
| phage display mediated immuno-pcr. | immuno-pcr (ipcr) is a powerful detection technology in immunological study and clinical diagnosis due to its ultrasensitivity. here we introduce a new strategy termed phage display mediated immuno-pcr (pd-ipcr). instead of utilization of monoclonal antibody (mab) and chemically bond dna that required in the conventional ipcr, a recombinant phage particle is applied as a ready reagent for ipcr experiment. the surface displayed single chain variable fragment (scfv) and phage dna themselves can di ... | 2006 | 16682441 |
| truncated recombinant dobrava hantavirus nucleocapsid proteins induce strong, long-lasting immune responses in mice. | we describe the cloning and expression of dobrava hantavirus (dobv) nucleocapsid proteins and a truncated form consisting of the first 118 n-terminal amino acids, and the capacity of these e. coli icone 200-expressed recombinant proteins (rnp) to induce a protective immune response against dobv in mice. as an alternative carrier protein, the outer membrane protein a derived from klebsiella pneumoniae (rp40) has been coupled to different rnp constructs. all recombinant proteins were found to be h ... | 2006 | 16714853 |
| characterization of truncated hantavirus nucleocapsid proteins and their application for serotyping. | hemorrhagic fever with renal syndrome (hfrs) is a fulminant infectious disease characterized by fever, hemorrhage, renal impairment, and thrombocytopenia. hantaviruses associated with this belong to different serotypes: hantaan (htn), seoul (seo), dobrava/belgrade (dob), and puumala (puu). the first two, htn and seo, are endemic in china. to investigate the epidemiology of hfrs and virus transmission in china, we constructed prokaryotic plasmids encoding truncated recombinant htn and seo nucleoc ... | 2006 | 16721853 |
| epidemiology of hemorrhagic fever with renal syndrome in endemic area of the republic of korea, 1995-1998. | we conducted an epidemiologic study to understand temporal and spatial patterns of hemorrhagic fever with renal syndrome (hfrs) in the republic of korea (rok). we estimated the incidence among civilians in endemic areas through the active surveillance system during the major epidemic periods, from september to december, between 1996 and 1998. we also estimated the prevalence among korean military personnel from 1995 to 1998. in addition, we assessed seroprevalence, subclinical infection rate, an ... | 2006 | 16891802 |
| genetic properties of medium (m) and small (s) genomic rna segments of seoul hantavirus isolated from rattus norvegicus and antigenicity analysis of recombinant nucleocapsid protein. | a novel isolate of seoul (seo) hantaviruses was detected and identified in rattus norvegicus in shandong province, china and designated as jun5-14. the partial m segment and the coding region of nucleocapsid protein (np) in the s segment of jun5-14 were pcr-amplified and sequenced. nucleotide sequence analysis of the partial m segment (300 bp) revealed that jun5-14 isolate was closely related to former seo isolates in shandong (97.3-99.0% homology) and non-shandong seo viruses (84.1-97.7% homolo ... | 2007 | 16927115 |
| clinical and laboratory predictors of oliguric renal failure in haemorrhagic fever with renal syndrome caused by hantaan virus. | haemorrhagic fever with renal syndrome (hfrs), caused by hantavirus infection, develops into acute renal failure (arf) of variable degrees of severity. we investigated the early predictive markers for oliguric arf in hfrs patients. | 2007 | 16930718 |
| hsp70 gene fused with hantavirus s segment dna significantly enhances the dna vaccine potency against hantaviral nucleocapsid protein in vivo. | heat shock proteins (hsps) have been shown to act as adjuvants when coadministered with peptide antigens or given as fusion proteins and enhance the vaccination efficiency. to evaluate the enhancement of the potency of hantaan virus (htnv) nucleocapsid protein (np) immunogenicity by heat shock protein 70 (hsp70), we developed a novel chimeric htnv s-hsp70 dna vaccine plasmid by genetically linking hsp70 gene to the full-length htnv s segment dna (htnv s dna). c57bl/6 mice were immunized with thi ... | 2007 | 16935395 |
| regions of importance for interaction of puumala virus nucleocapsid subunits. | puumala virus (puuv) is a hantavirus that causes a mild form of hemorrhagic fever with renal syndrome in northern and central europe, and in large parts of russia. the nucleocapsid (n) protein encoded by hantaviruses plays an important role in the life-cycle of these viruses, and one important function for the n-protein is to oligomerize, surround and protect the viral rnas. we have identified amino- and carboxy-terminal regions involved in puuv n-n interactions, which comprise amino acids 100-1 ... | 2006 | 16972031 |
| the pathogenic ny-1 hantavirus g1 cytoplasmic tail inhibits rig-i- and tbk-1-directed interferon responses. | hantaviruses cause two diseases with prominent vascular permeability defects, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. all hantaviruses infect human endothelial cells, although it is unclear what differentiates pathogenic from nonpathogenic hantaviruses. we observed dramatic differences in interferon-specific transcriptional responses between pathogenic and nonpathogenic hantaviruses at 1 day postinfection, suggesting that hantavirus pathogenesis may in part be de ... | 2006 | 16973572 |
| [hemorrhagic fever with the renal syndrome: modern aspects of epidemiology and prophylaxis]. | hemorrhagic fever with the renal syndrome (hfrs) is a typical natural-and-focal virus infection having military-and-epidemiological importance for armed forces of most countries. severe forms of this infection are typical for the far east of russia, countries of south-east asia and especially for the american continent. the lethality from hfrs amounts to 15-50%. the main natural carriers and keepers of hfrs stimulus are various mouse-type rodents. in russia besides well-studied natural focuses o ... | 2006 | 16977883 |
| hemorrhagic fever with renal syndrome (hfrs, korean hemorrhagic fever). | | 2007 | 16983539 |
| activity of ribavirin against hantaan virus correlates with production of ribavirin-5'-triphosphate, not with inhibition of imp dehydrogenase. | ribavirin (rbv) is a broad-spectrum antiviral agent that inhibits the production of infectious hantaan virus (htnv). although the mechanism of action of rbv against htnv is not understood, rbv is metabolized in human cells to both rbv-5'-monophosphate, which inhibits imp dehydrogenase, resulting in a decrease in intracellular gtp levels, and rbv-5'-triphosphate (rbv-tp), which could selectively interact with the viral rna polymerase. to elucidate which activity of rbv was most important to its a ... | 2007 | 17060520 |
| development of serological assays for thottapalayam virus, an insectivore-borne hantavirus. | thottapalayam virus (tpmv), a member of the genus hantavirus in the family bunyaviridae, was isolated from an insectivore, suncus murinus (musk shrew), captured in southern india in 1964. while the isolation of tpmv predates the discovery of the prototype hantaan virus, little is known about its genetics and biology. to date, preliminary evidence suggests that tpmv differs significantly, both antigenically and genetically, from all known rodent-borne hantaviruses. however, since detailed epizoot ... | 2007 | 17182762 |
| hantaan virus infection among human and rat populations in alexandria. | hantaan virus has recently been identified as the cause of the rodent borne viral zoonosis collectively termed hantavirus disease (hvd). although the disease is mainly endemic in two main areas, clinical forms as well as positive antibody testing from healthy persons throughout the world have been reported denoting a worldwide presence. rattus species are almost universally implicated as hantavirus reservoirs. screening for hantavirus antibodies was done on 637 samples of human sera and 861 rode ... | 1996 | 17217009 |
| the expression and genetic immunization of chimeric fragment of hantaan virus m and s segments. | hemorrhagic fever with renal syndrome (hfrs), which is characterized by severe symptoms and high mortality, is caused by hantavirus. there are still no effective prophylactic vaccines directed to hfrs until now. in this research, we fused expressed g2 fragment of m segment and 0.7kb fragment of s segment. we expect it could be a candidate vaccine. chimeric gene g2s0.7 was first expressed in prokaryotic expression system pgex-4t. after inducing expressed fusion proteins, gst-g2s0.7 was induced an ... | 2007 | 17266922 |
| degrons at the c terminus of the pathogenic but not the nonpathogenic hantavirus g1 tail direct proteasomal degradation. | pathogenic hantaviruses cause two human diseases: hantavirus pulmonary syndrome (hps) and hemorrhagic fever with renal syndrome (hfrs). the hantavirus g1 protein contains a long, 142-amino-acid cytoplasmic tail, which in ny-1 virus (ny-1v) is ubiquitinated and proteasomally degraded (e. geimonen, i. fernandez, i. n. gavrilovskaya, and e. r. mackow, j. virol. 77: 10760-10768, 2003). here we report that the g1 cytoplasmic tails of pathogenic andes (hps) and hantaan (hfrs) viruses are also degraded ... | 2007 | 17267477 |
| [the pathogenetic role of peripheral t-lymphocytes activation-induced cell death in hemorrhagic fever with renal syndrome]. | to study the activation-induced cell death (aicd) in peripheral blood t-lymphocytes (pbl-t) from patients of hemorrhagic fever with renal syndrome (hfrs) and the effect of aicd on the pathogenesis of hfrs. | 2006 | 17313872 |
| detection of circulating antigen with a mab-based sandwich-elisa and its comparison with specific igm detection in sera of patients with hemorrhagic fever with renal syndrome. | a monoclonal antibody (1h2) against nucleocapsid protein of hantavirus was developed. a sandwich enzyme-linked immunosorbent assay (elisa) with the monoclonal antibody (mab) was established and evaluated for detecting circulating antigen (cag) in serum of patients with hemorrhagic fever with renal syndrome (hfrs). results were compared to that of an immunoglobulin m (igm)-detecting elisa. of 143 patients with hfrs, 106 were positive for cag of hantavirus and 128 positive for specific igm. among ... | 2007 | 17316085 |
| ex vivo stability of the rodent-borne hantaan virus in comparison to that of arthropod-borne members of the bunyaviridae family. | the possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. in this study we have compared the stabilities of three viruses within the bunyaviridae family: the rodent-borne hantavirus hantaan virus (htnv), the sand fly-borne phlebovirus sandfly fever sicilian virus (sfsv), and the tick-borne nairovirus crimean-congo hemorrhagic fever virus (cchfv). these viruses differ in their transmission routes: sfsv and cchfv are vector bo ... | 2007 | 17337567 |
| defining the n-linked glycosylation site of hantaan virus envelope glycoproteins essential for cell fusion. | the hantaan virus (htnv) is an enveloped virus that is capable of inducing low ph-dependent cell fusion. we molecularly cloned the viral glycoprotein (gp) and nucleocapsid (np) cdna of htnv and expressed them in vero e6 cells under the control of a cmv promoter. the viral gene expression was assessed using an indirect immunofluorescence assay and immunoprecipitation. the transfected vero e6 cells expressing gps, but not those expressing np, fused and formed a syncytium following exposure to a lo ... | 2007 | 17342054 |
| a comparative epidemiological study of hantavirus infection in japan and far east russia. | hantaviruses are causative agents of some severe human illnesses, including hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). the viruses are maintained by rodent hosts, and humans acquire infection by inhaling virus-contaminated excreta from infected animals. to examine the epidemiology of hantavirus infections in japan and far east russia, we conducted epidemiological surveys in these regions. in japan, anti-hantavirus antibodies were found in four rodent sp ... | 2007 | 17405352 |
| analysis of the immune response of hantaan virus nucleocapsid protein-specific cd8+ t cells in mice. | the major histocompatibility complex (mhc) class-i restricted epitope of hantaan virus nucleocapsid protein (n) was identified using overlapping peptides and balb/c mice. using the mhc tetramer derived from the epitope, we found that the level of n-specific cd8(+) t cells increased to approximately 20% of all antigen-specific cd8(+) t cells in a mouse model of transient infection. however, n-specific cd8(+) t cells were undetectable in a mouse model of persistent infection, both in the persisten ... | 2007 | 17481691 |
| mxa-independent inhibition of hantaan virus replication induced by type i and type ii interferon in vitro. | interferons (ifn) induce an antiviral state against hantaan virus (htnv) but the mechanisms responsible for inhibition are unclear. the ifn-inducible mxa is discussed to be important for control of infections with hantaviruses. to characterize the role of endogenous mxa, the inhibition of htnv induced by type i and type ii ifns was compared in vero and a549 cells. ifnalpha and ifngamma reduced production of infectious virions, viral rna, and nucleocapsid protein with the same efficiency, althoug ... | 2007 | 17482705 |
| [screening of biopolymers from sea hydrocoles affecting the adsorption of hantaan virus]. | the biopolymers extracted from sea hydrocoles were screened and a group of sulfated polysaccharides--fucoidans having a pronounced inhibitory effect on the adsorption of hantaan virus in the cultured vero e6 cells was identified. the mechanism of action of sulfated polysaccharides was realized through competitive carbohydrate-specific binding to cell membrane receptors and through ligand-receptor interaction and blocking of hantaan virus glycoproteins (g1 and g2). | 2007 | 17500236 |
| lambda interferon (ifn-lambda) in serum is decreased in hantavirus-infected patients, and in vitro-established infection is insensitive to treatment with all ifns and inhibits ifn-gamma-induced nitric oxide production. | hantaviruses, causing hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps), are known to be sensitive to nitric oxide (no) and to pretreatment with type i and ii interferons (alpha interferon [ifn-alpha]/ifn-beta and ifn-gamma, respectively). elevated serum levels of no and ifn-gamma have been observed in hfrs patients, but little is known regarding the systemic levels of other ifns and the possible effects of hantaviruses on innate antiviral immune respons ... | 2007 | 17522204 |
| nucleotide sequence characterization and phylogenetic analysis of hantaviruses isolated in shandong province, china. | china is the most severe endemic area of hemorrhagic fever with renal syndrome (hfrs) in the world with 30,000-50,000 cases reported annually, which accounts for more than 90% of total number of cases worldwide. the incidence rate of the syndrome in shandong province is one of the highest in china, which has ever reached 50 per 100,000 persons per year. however, the molecular characteristics of hantaviruses (hv) epidemic in shandong province remain unclear. therefore it is useful to clarify nucl ... | 2007 | 17531126 |
| dynein-dependent transport of the hantaan virus nucleocapsid protein to the endoplasmic reticulum-golgi intermediate compartment. | in contrast to most negative-stranded rna viruses, hantaviruses and other viruses in the family bunyaviridae mature intracellularly, deriving the virion envelope from the endoplasmic reticulum (er) or golgi compartment. while it is generally accepted that old world hantaviruses assemble and bud into the golgi compartment, some studies with new world hantaviruses have raised the possibility of maturation at the plasma membrane as well. overall, the steps leading to virion assembly remain largely ... | 2007 | 17537852 |
| [prevalence of antibodies to hantavirus among hemodialysis patients with end-stage renal failure in kaunas and its district]. | the objective of this study was to investigate the prevalence of antibodies to hantaviruses among hemodialysis patients with end-stage renal failure in kaunas and its district. | 2007 | 17551280 |