Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [a study on immunogenicity and safety of bivalent inactivated vaccine against hemorrhagic fever with renal syndrome]. | to study side effects and effectiveness of bivalent inactivated vaccine against hemorrhagic fever with renal syndrome in population of a randomized controlled field trial. | 1999 | 11864504 |
| relationship between the clinical features and the viral antigen in the extremity blood of the patients with hemorrhagic fever with renal syndrome. | the direct immunogold silver staining (d-igss) method was used to detect the viral antigen in the extremity blood of 67 cases of hemorrhagic fever with renal syndrome. the positive rate of viral antigen was the highest during the fever, hypotension and oligouria phrase; and the rate dropped gradually during the polyuria and convalescent phase. it is suggested that clinical staging was positively related with the percentage of the viral antigen positive cells (p < 0.001). it is concluded that the ... | 1999 | 12840882 |
| identification of h-2k(b)-restricted t-cell epitopes within the nucleocapsid protein of hantaan virus and establishment of cytotoxic t-cell clones. | although neutralizing antibodies against hantaan virus (htv) can protect hosts from viral infection, t-cell responses to htv are also important in host defense against htv. however, much less is known about cytotoxic t lymphocyte (ctl) responses to htv. to identify ctl epitopes in the htv nucleocapsid protein (np), we selected 7 h-2k(b)-motif-fitting peptides. of these peptides, 3 peptides (np3, np4, and np7) were recognized by ctl responses derived from htv-immunized mouse splenocytes. np3 and ... | 2000 | 10596020 |
| study on biologic activity for membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus. | using dph fluorescence probe, the membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus (ehfv) was labeled. the membrane lipid fluidity was obviously decreased from the membrane lipid fluorescence polarization. the membrane lipid fluidity of lymphocyte, monocyte and neutrophilic granulocyte was dynamically observed. after culturing the cells for 1, 6, 24 and 72 h, it was found that all the membrane lipid fluidity of the infected cells was decreased obviously wit ... | 2000 | 12840924 |
| [isolation, identification and certain characteristics of a hantaan virus proposed as candidate virus for killed vaccine]. | isolation and identification of a hantaan virus from hfrs patient's serum for killed vaccine development. | 2000 | 11503020 |
| [a study on the epidemiology and preventive measure on epidemic haemorrhage fever in army barrack area in shandong]. | in order to guide the army and civilians to carry out the prevention on epidemic haemorrhage fever (ehf), it's necessary to elucidate the epidemic feature of natural epidemic foci and the risk factors of ehf in the army - stationed areas. | 2000 | 11860754 |
| [preventive effects of three kinds of inactive vaccines against epidemic hemorrhagic fever (ehf) after 5 years of vaccination]. | to observe the safety and the preventive effects of three kinds of vaccines as mongolian gerbils kidney vaccine, mouse brains vaccine and hamster kidney vaccine inoculated 5 years ago. | 2000 | 11860813 |
| [study on the genetic difference of seo type hantaviruses]. | to understand the genetic type of hantaviruses and the difference between them caused by rodents in beijing and to furhter explore the source of the infectious factors. | 2000 | 11860814 |
| [safety and immunogenicity of inactivated bivalent ehf vaccine in humans]. | safety and immunogenicity of inactivated bivalent ehf vaccine in humans were evaluated in the epidemic area of zhejiang province, china. | 2000 | 11860832 |
| [report on epidemic hemorrhagic fever (ehf) surveillance in 1999]. | to understand the epidemic tread of epidemic hemorrhagic fever (ehf) in 1999 and to develop programs for its control and prevention. | 2000 | 11860833 |
| [establishment of a hantaan virus infected mouse neuron cell model]. | to establish a neuron cells model for hantaan virus (htnv) infection by using the cultures of cortical neurons of mouse embryo. changes and mechanism of the damage in neurons after htnv infection were studied. | 2000 | 11498693 |
| clinical evaluation of a vaccinia-vectored hantaan virus vaccine. | we evaluated a vaccinia-vectored vaccine for hemorrhagic fever with renal syndrome in clinical trials. a phase i dose-escalation study in 16 volunteers divided into four groups demonstrated that subcutaneous inoculation of approximately 10(7) plaque-forming units of the recombinant virus was safe and immunogenic. vaccination of a fifth group of 12 volunteers indicated that neutralizing antibody titers to both vaccinia virus and hantaan virus were enhanced after a second inoculation. comparing tw ... | 2000 | 10568767 |
| persistent hantavirus infections: characteristics and mechanisms. | hantaviruses include serious human pathogens that are maintained in nature in persistently infected rodents and that can also persistently infect cultured mammalian cells, causing little or no cytopathology. the mechanisms of hantavirus persistence are only beginning to be explored. recent data point to subtle changes in the viral genome that might result in the differential regulation of replication and lead to persistence. | 2000 | 10664598 |
| detection of hantaan and seoul viruses by reverse transcriptase-polymerase chain reaction (rt-pcr) and restriction fragment length polymorphism (rflp) in renal syndrome patients with hemorrhagic fever. | hemorrhagic fever with renal syndrome (hfrs), also called korean hemorrhagic fever (khf), is the most common cause of acute renal failure in the far east. two serotypes of hantavirus, hantaan and seoul viruses are known pathogens for hfrs in korea. | 2000 | 10711409 |
| [acute renal failure in hantavirus infections]. | background: the acute renal failure remains a diagnostic challenge for the clinician. case reports: between 1991 and 1996, acute renal failure caused by hantavirus infection was diagnosed in 4 previously healthy male patients. main symptoms consisted of fever, headache, arthralgia, lumbar and abdominal pain as well as a decline in diuresis. the ultrasonography showed a slight splenomegaly in 2 patients. the clinical chemistry showed elevated serum creatinine from 2.2 mg/dl to 6.7 mg/dl and throm ... | 2000 | 10808304 |
| detection of hantaviral antibodies among patients with hepatitis of unknown etiology in japan. | hantaviral antibodies were detected in the sera from patients with hepatic disease of unknown etiology in japan by several different serological diagnostic methods. a total of 105 sera from diseased patients which were negative to a-g hepatitis virus infections in the tokyo area were tested. among them, 3 out of 73 sera from patients with chronic hepatic disease were positive to hantaviral antibody by enzyme-linked immunosorbent assay (elisa), indirect immunofluorescent antibody assay (ifa) and ... | 2000 | 10888353 |
| genetic diversity, distribution, and serological features of hantavirus infection in five countries in south america. | since 1995 when the first case of hantavirus pulmonary syndrome (hps) was reported in patagonia, there have been more than 400 cases of hps reported in five countries in south america. the first case of hps was associated with andes (and) virus. in this study, we report on the genetic diversity, geographical distribution, and serological features of hantavirus infection in six countries in south america based on 87 hps cases from argentina, bolivia, chile, paraguay, and uruguay. an early immunog ... | 2000 | 10921972 |
| [outbreak of hemorrhagic fever with kidney syndrome in egor'ev district of moscow region]. | epizootological, serological, and molecular virological analysis of an outbreak of hemorrhagic fever with renal syndrome (hfrs) in the egoryevsk district of moscow region (september 1995-january 1996) has been carried out. hantavirus (puumala) antigen and virus-specific antibodies were isolated from bank voles captured in the endemic focus. anti-puumala antibodies were detected in the sera of all hfrs patients and in 2% healthy residents of the endemic focus. analysis of nucleotide sequence (rna ... | 2000 | 10971964 |
| pathogenicity of hantaan virus in newborn mice: genetic reassortant study demonstrating that a single amino acid change in glycoprotein g1 is related to virulence. | two hantaan virus strains, clone 1 (cl-1), which is virulent in newborn mice, and its attenuated mutant (mu11e10), were used to examine the pathogenesis of hantaan virus infection in a mouse model and identify virus factors relating to virulence. after subcutaneous inoculation of newborn balb/c mice, cl-1 caused fatal disease with high viral multiplication in peripheral organs, but mu11e10 produced nonfatal infection with a low level of virus multiplication. intracerebral inoculation of either s ... | 2000 | 10982372 |
| evaluation of two commercially available immunoassays for the detection of hantavirus antibodies in serum samples. | hantaviruses are members of the family bunyaviridae and the spectrum of clinical symptoms in humans may vary from sub-clinical to severe haemorrhagic fever with renal syndrome (hfrs) or pulmonary syndrome (hps). several serotypes have been described from which at least five are pathogenic to humans. each serotype has a different animal reservoir and geographical distribution. in the acute phase of the disease the clinical diagnosis may be confirmed by serology or by polymerase-chain reaction (pc ... | 2000 | 10996116 |
| genetic diversity of hantaviruses associated with hemorrhagic fever with renal syndrome in the far east of russia. | to identify the hantaviruses causing hemorrhagic fever with renal syndrome (hfrs) in the far east of russia, blood samples collected from hfrs patients in 1994-1998, were examined by reverse transcription-polymerase chain reaction. in addition, 36 sera were tested by an immunofluorescence assay for antibodies against hantaan, seoul, puumala, and khabarovsk viruses, and 54 samples were tested by plaque reduction neutralization test. with both serological assays, the highest antibody titers were t ... | 2000 | 11074123 |
| genetic diversity of hantaviruses isolated in china and characterization of novel hantaviruses isolated from niviventer confucianus and rattus rattus. | the antigenic and genetic properties of 46 hantaviruses from china, 13 from patients, 23 from rodents, and 10 from unknown hosts, were compared with those of other hantaviruses. the viruses were classified as either hantaan (htn) or seoul (seo) viruses. a phylogenetic analysis of the partial m (300 bp) and s (around 485 bp) genomes of htn viruses identified nine distinct genetic subtypes, one consisting of isolates from korea. the seo viruses were divided into five genetic subtypes, although the ... | 2000 | 11118357 |
| genetic diversity of apodemus agrarius-borne hantaan virus in korea. | hantaan (htn) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (hfrs), was first isolated in 1976 from lung tissues of striped field mice (apodemus agrarius) captured in songnae-ri, kyungki-do, korea. to clarify the genetic diversity and phylogenetic relationship among korean strains of htn virus, viral sequences of the partial s and m segments were amplified from lung tissues of 24 seropositive striped field mice captured between 1989 and 1998 at 11 sites in ... | 2000 | 11129640 |
| hantavirus nucleocapsid protein is expressed as a membrane-associated protein in the perinuclear region. | black creek canal virus (bccv) is a new world hantavirus which is associated with hantavirus pulmonary syndrome. we have examined the site of expression of the bccv nucleocapsid protein (nbccv) in the absence of bccv glycoproteins and found that the majority of the protein is localized to the golgi region. immunofluorescence analysis of bhk21 cells expressing the nbccv and la crosse virus nucleocapsid protein (nlacv) showed different intracellular localization patterns of these proteins within t ... | 2001 | 11160679 |
| clinical manifestations of new world hantaviruses. | 2001 | 11217400 | |
| cis-acting signals in encapsidation of hantaan virus s-segment viral genomic rna by its n protein. | the nucleocapsid (n) protein encapsidates both viral genomic rna (vrna) and the antigenomic rna (crna), but not viral mrna. previous work has shown that the n protein has preference for vrna, and this suggested the possibility of a cis-acting signal that could be used to initiate encapsidation for the s segment. to map the cis-acting determinants, several deletion rna derivatives and synthetic oligoribonucleotides were constructed from the s segment of the hantaan virus (htnv) vrna. n protein-rn ... | 2001 | 11222687 |
| evaluation of a pan-reactive hantavirus enzyme immunoassay and of a hantavirus immunoblot for the diagnosis of nephropathia epidemica. | nephropathia epidemica (ne) caused by the hantavirus serotype puumala (puuv) is endemic in large parts of europe. the prognosis of this disease is usually good. however, a rapid serological diagnosis is important to differentiate ne from potentially more severe renal conditions. | 2001 | 11255099 |
| [epidemiology of hantaviruses in baden-wurttemberg]. | hantavirus, originally named after the hantaan river in korea, is the aetiologic agent for the hemorrhagic fever with renal syndrome (hfrs) in the asian region, in the americas for the hantavirus pulmonary syndrome (hps). in middle europe hantaviruses are responsible for the "nephropathia epidemica" (ne), a mild form of hfrs. hantaviruses belong to the family of bunyaviridae. like other members of this family their genome consists of three segments of single stranded rna (ss-rna) leading to var ... | 2001 | 11285748 |
| genetic and serotypic characterization of sin nombre-like viruses in canadian peromyscus maniculatus mice. | in canada, hantavirus infected deer mice (peromyscus maniculatus) have been collected from british columbia to newfoundland. partial sequencing of g1 and n protein encoding regions from canadian peromyscus maniculatus-borne hantaviruses demonstrated the existence of significant genotypic divergence among strains. phylogenetic analysis showed that sin nombre (sn)-like viruses from eastern and western canadian deer mice can be divided into at least two broad-based genogroups. sequencing of mitocho ... | 2001 | 11311430 |
| molecular cloning and high-level expression of g2 protein of hantaan (htn) virus 76-118 strain in the yeast pichia pastoris km71. | hantaan viral g2 envelope gene, which is known to be one of major antigens and induce neutralizing antibodies, was cloned into expression vector phil-s1 which consists of aox1 promoter, pho1 signal sequence, his4 gene and other components. the recombined plasmid was transformed into methylotropic yeast, pichia pastoris of km71 and recombinant strains harboring multi-copy of g2 gene were selected. expression of the cloned g2 gene was confirmed with western blot analysis using anti-sera of guinea ... | 2001 | 11324753 |
| hantavirus infection induces the expression of rantes and ip-10 without causing increased permeability in human lung microvascular endothelial cells. | sin nombre virus (snv) and hantaan virus (htn) infect endothelial cells and are associated with different patterns of increased vascular permeability during human disease. it is thought that such patterns of increased vascular permeability are a consequence of endothelial activation and subsequent dysfunction mediated by differential immune responses to hantavirus infection. in this study, the ability of hantavirus to directly induce activation of human lung microvascular endothelial cells (hmve ... | 2001 | 11390609 |
| dna vaccination with the hantaan virus m gene protects hamsters against three of four hfrs hantaviruses and elicits a high-titer neutralizing antibody response in rhesus monkeys. | four hantaviruses-hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv) and puumala virus-are known to cause hemorrhagic fever with renal syndrome (hfrs) in europe and asia. htnv causes the most severe form of hfrs (5 to 15% case-fatality rate) and afflicts tens of thousands of people annually. previously, we demonstrated that dna vaccination with a plasmid expressing the seov m gene elicited neutralizing antibodies and protected hamsters against infection with seov and htnv. here, we r ... | 2001 | 11507192 |
| interaction between molecules of hantavirus nucleocapsid protein. | intermolecular interactions of tula hantavirus n (nucleocapsid) protein were detected in the yeast two-hybrid system, prompting further attempts to study this phenomenon. using chemical cross-linking and immunoblotting it was shown that the n protein from purified virus and from infected cell lysates as well as recombinant protein produced in a baculovirus expression system are capable of forming dimers, trimers and multimers, thus confirming the capacity of the protein molecules to interact wit ... | 2001 | 11457990 |
| clinical characterization of dobrava hantavirus infections in germany. | there is increasing evidence that dobrava (dobv) but not hantaan (htnv) hantavirus is a hemorrhagic fever with renal syndrome (hfrs) causing agent in central europe. however, only single clinical cases of hfrs due to acute dobv infection have been described so far. we report on three male patients from a non-endemic hantavirus focus in northern germany who suffered from mild to moderate hfrs strongly resembling nephropathia epidemica. serotyping by detection of hantavirus species-specific neutra ... | 2001 | 11393382 |
| truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections. | truncated recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), seoul virus (seov), and dobrava virus (dobv) were expressed by a baculovirus system. the truncated rnps, which lacked 49 (rnp50) or 154 (rnp155) n-terminal amino acids of the nps of htnv, seov, and dobv, were able to differentiate htnv-, seov-, and dobv-specific immune sera. recombinant np50s retained higher reactivities than rnp155s and were proven useful for enzyme-linked immunosorbent assay (elisa). the elisas based o ... | 2001 | 11427545 |
| the hantaan virus glycoprotein precursor is cleaved at the conserved pentapeptide waasa. | the medium segment of the tripartite negative-stranded rna genome of hantaviruses encodes for the predicted glycoprotein precursor gpc. we have demonstrated here the expression of the glycoprotein precursor of hantaan virus following transfection of mammalian cells. the cleavage of the precursor into the glycoproteins g1 and g2 followed the rules for signal peptides and seemed to occur directly at the pentapeptide motif "waasa." our data indicate that the signal peptidase complex is responsible ... | 2001 | 11689045 |
| a dominant antigenic region of the hantaan virus nucleocapsid protein is located within a amino-terminal short stretch of hydrophilic residues. | the nucleocapsid (n) protein of the hantaan virus (htnv) is a major viral antigen that induces a strong antibody response during the acute phase of infection. by immunoblot analyses of the recombinant n proteins using human sera of the hemorrhagic fever with renal syndrome (hfrs), we have confirmed previous finding by other investigators of the presence of a highly antigenic region near the amino terminus of the htnv n protein. we have further located the antigenic region within a short stretch ... | 2001 | 11724272 |
| [experimental study on the roles of gasmid mite and chigger mite in the transmission of hemorrhagic fever with renal syndrome virus]. | to study the roles of gasmid mite and chigger mite in the transmission of hemorrhagic fever with renal syndrome virus (hfrsv). | 2001 | 11769690 |
| [comparative study on antibody levels of vaccination group and control group after 4 years of immunized with type b inactivated vaccine against hemorrhagic fever with renal syndrome]. | to compare the antibody level of vaccine group and control group in a randomized, controlled trial of type b inactivated vaccine against hemorrhagic fever with renal syndrome(hfrs) in jiande county of zhejiang province and to find out if there is enhancement of subclinical infection about type b inactivated vaccine against hfrs. | 2001 | 11986705 |
| [central hemodynamic disorders and rheological red blood cell properties in hemorrhagic fever patients with renal syndrome]. | the aim of this study was to detect a relationship between hemodynamic disorders in patients with hemorrhagic fever with the renal syndrome (hfrs) and erythrocyte aggregability and erythrocyte membrane atpase activity. a total of 100 patients with hfrs of different severity were examined. central hemodynamic parameters were studied: circulating blood volume, minute volume, cardiac index, stroke volume, and total peripheral vascular resistance during preoliguria, oliguria, and polyuria periods. b ... | 2001 | 11510183 |
| [seroepidemiologic study on hemorrhagic fever with renal syndrome virus in the migration areas of the three-gorges reservoir chongqing, region, yangtze river]. | to ascertain both the immune level and latent infection status among the migrants from the three-gorge reservoir, chongqing region. | 2001 | 11718068 |
| increased expression of hsp70 and co-localization with nuclear protein in cells infected with the hantaan virus. | to investigate the effect of hantaan virus infection on the expression of stress genes. | 2001 | 11780421 |
| primary humoral immune responses to formalin inactivated hemorrhagic fever with renal syndrome vaccine (hantavax): consideration of active immunization in south korea. | the efficacy of a formalin-inactivated hemorrhagic fever with renal syndrome (hfrs) vaccine and the effectiveness of a related vaccination program have not been previously evaluated. we measured the primary immune responses to hantavax by plaque reduction neutralizing antibody test (prnt), hemagglutination inhibition test (hai), elisa and high density particle agglutination test (hdpa) in order to confirm a possible biological efficacy through independent substantiation of experimental results a ... | 2001 | 11456392 |
| sonographic evaluation of gallbladder-wall thickening in hemorrhagic fever with renal syndrome: prediction of disease severity. | gallbladder-wall thickening (gbwt) frequently occurs in patients with hemorrhagic fever with renal syndrome (hfrs), an acute infectious disease caused by hantaviruses. hfrs is manifested by fever, hemorrhage, renal failure, and in many cases gastrointestinal symptoms, such as abdominal pain and tenderness. the clinical significance of gbwt in hfrs has not been reported. the purpose of this study was to investigate the incidence of gbwt and the relationship between gbwt and the severity of hfrs. | 2001 | 11486323 |
| on the history of the study of haemorrhagic fever with renal syndrome in eastern russia. | 2001 | 11390737 | |
| [study on using colloidal gold immuno-dot assay to detect special antibody of hemorrhagic fever renal syndrome]. | to seek for a simple, sensitive and rapid assay to detect specific antibody of patients suffering from hemorrhagic fever renal syndrome (hfrs). | 2001 | 12575393 |
| [laboratory diagnosis of hemorrhagic fever with renal syndrome in the ulianovsk region]. | specific laboratory diagnosis is of great importance for the diagnosis of hemorrhagic fever with renal syndrome (hfrs). the results of the investigation confirm the persistence of the specific antibodies in blood sera of the patients with hfrs. the indices of the serologic confirmation of the diagnosis of hfns are proven to elevate if the optimal intervals of sampling the pair blood sera is observed. | 2002 | 12271728 |
| hantaan virus antibody prevalence in rodent populations of several provinces of northeastern thailand. | we conducted a serological survey of 632 rodents from the northeast region of thailand in order to assess the presence of hantaan-like viruses that may be a risk to the human population. rodents were collected from rice fields, houses and domestic gardens in five northeastern provinces and tested for igg reacting sera to hantaan antigen using enzyme-linked immunoassays. the overall prevalence of hantavirus infection in rodents was 2.1% (13/632). species that tested positive included bandicota in ... | 2002 | 12358618 |
| hantavirus infection of dendritic cells. | dendritic cells (dcs) play a pivotal role as antigen-presenting cells in the antiviral immune response. here we show that hantaan virus (htnv), which belongs to the bunyaviridae family (genus hantavirus) and causes hemorrhagic fever with renal syndrome, productively infects human dcs in vitro. in the course of htnv infection, dcs did not show any cytopathic effect and viral replication did not induce cell lysis or apoptosis. furthermore, htnv did not affect apoptosis-inducing signals that are im ... | 2002 | 12368315 |
| pathogenic and nonpathogenic hantaviruses differentially regulate endothelial cell responses. | hantaviruses cause two human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hantaviruses infect human endothelial cells but cause little or no damage to the infected endothelium. we analyzed with affymetrix dna arrays (santa clara, ca) the endothelial cell transcriptional responses directed by hantaviruses associated with hps [new york-1 virus (ny-1v)], hfrs [hantaan virus (htnv)], or by a hantavirus not associated with human disease [prospect hil ... | 2002 | 12368479 |
| the in vitro and in vivo protective activity of monoclonal antibodies directed against hantaan virus: potential application for immunotherapy and passive immunization. | hantaan virus (htnv), a member of the genus hantavirus, family bunyaviridae, is an etiologic agent causing a serious human disease, hemorrhagic fever with renal syndrome (hfrs), with a mortality as high as 15% and is also a potential bioterrorism agent. it is urgently needed to develop anti-htnv-neutralizing monoclonal antibodies (mabs) for treatment and prevention of htnv infection. in the present study, 18 murine mabs directed against htnv strain chen were generated and characterized. among th ... | 2002 | 12408987 |
| serological study of hantavirus in man in the autonomous community of madrid, spain. | data relating to hantavirus infection in spain are scarce and limited to rural areas. the aim of this work was to study the seroprevalence of hantavirus infection in the autonomous community of madrid (acm), a region containing both rural and urban populations in different ecological settings. sera from 3852 individuals (1849 male, 2003 female) were screened by indirect inmunofluorescence, with vero e6 cells infected with puumala, hantaan and seoul viruses as antigens. screen-positive results we ... | 2002 | 12435066 |
| phylogenetic evidence for host switching in the evolution of hantaviruses carried by apodemus mice. | phylogenetic analysis of three hantaviruses: hantaan (htnv), dobrava (dobv), and the newly designated serotype/genotype saaremaa (saav) and their respective hosts, rodents of genus apodemus, reveals a discrepancy in the virus-host relationships. while all apodemus agrarius sequences from europe and the far east are monophyletic, saav (carried by the western subspecies of a. agrarius) shared the most recent ancestor with a. flavicollis-associated dobv virus, but not with htnv (carried by the east ... | 2002 | 12457975 |
| [efficacy of amixine in experimental hantavirus infection]. | the experimental studies conducted on 2-week suckling mice infected with hantaan virus, strain 76-118) treated with oral and subcutaneous amoxine showed its prophylactic, therapeutical-and-prophylactic, and therapeutical efficiencies. oral amoxine exhibited the highest efficiency when used in a dose of 10 mg/kg-1 96 hours before infection and throughout the incubation period. the protective efficiency was 61%. subcutaneously, the agent was effective when three schemes for injection in a dose of ... | 2002 | 12522965 |
| [anthropogenic transformation of the environment and morbidity of infections with natural focality in the ul'ianovsk region]. | 2002 | 12506644 | |
| [purification and identification of recombinant nuclear protein of hantaan virus]. | to purify recombinant nuclear protein of hantaan virus. | 2002 | 12665907 |
| [clinical study on intrauterine hemorrhagic fever with renal syndrome virus infection]. | to find out the status of intrauterine infection with hemorrhagic fever with renal syndrome virus (hfrsv). | 2002 | 12665941 |
| [analysis of hfrs vaccination against hemorrhagic fever with renal syndrome during 1999-2000 in laiwu city]. | to evaluate the effect the vaccine of hemorrhagic fever with renal syndrome (hfrs). | 2002 | 12665942 |
| hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis. | the cellular entry of hantaan virus (htn) occurs through interactions with beta(3) integrins as cellular receptors. however, the process of htn infection following attachment to the cell surface is not well understood. our data indicate that overexpression of a dominant-negative mutant dynamin inhibits htn internalization and that compounds that block clathrin- but not caveolae-dependent endocytosis also reduce htn infectivity. in addition, we show that htn colocalizes with the clathrin heavy ch ... | 2002 | 11886265 |
| characterization of hla-a2.1-restricted epitopes, conserved in both hantaan and sin nombre viruses, in hantaan virus-infected patients. | nine different ctl epitopes, conserved in both hantaan virus (htnv) and sin nombre virus (snv), were selected for study. the binding affinity of each peptide with hla-a2.1 molecules in vitro was determined and antigen-specific responses from seven donors who had a previous field infection with htnv were examined. although the strength or frequency of ctl activity showed different patterns in the seven patients, five of seven patients showed significant activity against at least one or more epito ... | 2002 | 11961268 |
| [a preliminary genetic reassortment between hantaan virus and seoul virus strains]. | to determine the frequency and characteristics of reassortment among hantaan and seoul viruses causing hemorrhagic fever with renal syndrome (hfrs). | 2002 | 12015110 |
| [complete genome sequence of 84fli, a hantaan virus strain isolated from the liver of fetus aborted by a pregnant women with hemmorrhagic fever with renal syndrome]. | to study the complete genome sequence of the chinese hantaan virus vaccine strain 84fli and learn about its molecular characters. | 2002 | 12181094 |
| haemorrhagic fever with renal syndrome in croatia. | 2002 | 12241810 | |
| [distribution of hemorrhagic fever with renal syndrome virus in gamasid mites and chigger mites]. | to study the distribution of hemorrhagic fever with renal syndrome virus (hfrsv) in mites. | 2002 | 12411201 |
| [proliferation and location of hantaan virus in gamasid mites and chigger mites, a molecular biological study]. | to study the proliferation and location of hantaan virus (hv) in gamasid mites and chigger mites and the significance of gamasid mites and chigger mites as vectors of transmission of hemorrhagic fever with renal syndrome (hfrs). | 2002 | 12509926 |
| [discovery of two sub-types of hantanvirus in anhui mountain areas]. | to investigate the existence of hemorrhagic fever with renal syndrome (hfrs) foci in high mountains and forest areas. | 2002 | 12482368 |
| application of cdna microarray technique to detection of gene expression in host cells infected with viruses. | cdna microarray technique was used to monitor changes in mrna levels in cells after hantaan virus (htnv) infection. the values of the ratio of medians for htnv and japanese encephalitis virus (jev) at the early stage of infection were compared and found similar, suggesting that the same or similar genes are associated with the early events of infection with either virus. the reproducibility of values of the "ratio of medians" for htnv was examined. we found that applying cluster analysis to the ... | 2002 | 12580375 |
| hantavirus nucleocapsid protein coiled-coil domains. | the nucleocapsid (n) proteins of hantaviruses such as the sin nombre virus (snv) bind to membranes and viral rnas, associate with transcription and replication complexes, and oligomerize during the process of virus assembly. n proteins trimerize in vitro and in vivo, and associate via assembly domains at their amino- and carboxyl-terminal ends. because structure prediction algorithms suggested that n protein residues 3-75 form two coiled-coil motifs separated by an intervening kink or turn seque ... | 2002 | 12019266 |
| genetics of hantaviruses: implications to taxonomy. | hantaviruses (genus hantavirus, family bunyaviridae) represent a prime example of emerging viruses. since isolation of the prototype hantaan virus in the late 70s more than 20 new species have been described and the number is increasing fast thus demanding for a more refined classification. taking into account that hantaviruses are difficult to isolate in cell culture, one should not be surprised that most of the "newcomers" were first described as distinct hantavirus genotypes. moreover, the on ... | 2002 | 12038679 |
| hantaan virus infection causes an acute neurological disease that is fatal in adult laboratory mice. | hantaan virus, the etiological agent of korean hemorrhagic fever, is transmitted to humans from persistently infected mice (apodemus agrarius), which serve as the primary reservoir. here we demonstrate that several strains of adult mus musculus domesticus (c57bl/6, balb/c, akr/j, and sjl/j) were susceptible to hantaan virus infection when infected intraperitoneally. first clinical signs were loss of weight, ruffled fur, and reduced activity, which were followed by neurological symptoms, such as ... | 2002 | 12163608 |
| golgi localization of hantaan virus glycoproteins requires coexpression of g1 and g2. | the membrane glycoproteins g1 and g2 of hantaan virus (htnv; family bunyaviridae) are encoded on the m rna genome segment as a precursor polypeptide that is cotranslationally cleaved by host proteases. g1 and g2 accumulate in the golgi complex in cells following either virus infection or transfection with m segment cdna. however, there are conflicting reports in the literature concerning the golgi targeting of separately expressed g1. to resolve these differences, a series of m segment and g1 co ... | 2002 | 12202203 |
| [bunyavirus--virus and host relationship: the coevolution between hantavirus and rodent]. | 2002 | 12227181 | |
| the rna binding domain of the hantaan virus n protein maps to a central, conserved region. | the nucleocapsid (n) protein of hantaviruses encapsidates both viral genomic and antigenomic rnas, although only the genomic viral rna (vrna) is packaged into virions. to define the domain within the hantaan virus (htnv) n protein that mediates these interactions, 14 n- and c-terminal deletion constructs were cloned into a bacterial expression vector, expressed, and purified to homogeneity. each protein was examined for its ability to bind the htnv s segment vrna with filter binding and gel elec ... | 2002 | 11884555 |
| transfection-mediated generation of functionally competent tula hantavirus with recombinant s rna segment. | since the discovery of rna recombination in polioviruses, there has been a general belief that this mechanism operates only in positive-sense rna viruses. recently, studying wild-type tula hantavirus, we observed a mosaic-like structure of the s rna segment that was consistent with generation by recombination between viruses from two genetic lineages. here we show transfection-mediated rescue of tula virus carrying recombinant s rna segment. independent attempts yielded s rna molecules of simila ... | 2002 | 11889055 |
| hemorrhagic fever with renal syndrome presenting with hemophagocytic lymphohistiocytosis. | hemophagocytic lymphohistiocytosis--which is associated with a variety of infections, malignant neoplasms, autoimmune diseases, and immunodeficiencies--is an uncommon syndrome with a rapidly fatal outcome. we describe the first case of hemorrhagic fever with renal syndrome due to hantaan virus presenting with reactive hemophagocytosis. | 2002 | 11897077 |
| serologic diagnosis of hantaan virus infection based on a peptide antigen. | 2002 | 11901064 | |
| identification of a cell surface 30 kda protein as a candidate receptor for hantaan virus. | cellular receptors play an important role in virus pathogenesis. as a first step in virus infection, viruses attach to specific receptors on the surface of cells; hantaan virus infects susceptible cells by attaching to a receptor located on the cell surface. to date, the identity of the hantaan virus host cell receptor remains unknown. to determine the protein on the cell surface to which hantaan virus binds, a virus overlay protein-binding assay was performed with radiolabelled virus. a 30 kda ... | 2002 | 11907325 |
| [research and control of hantaan virus and hfrs]. | 2003 | 15043085 | |
| phylogenetic analysis reveals a low rate of homologous recombination in negative-sense rna viruses. | recombination is increasingly seen as an important means of shaping genetic diversity in rna viruses. however, observed recombination frequencies vary widely among those viruses studied to date, with only sporadic occurrences reported in rna viruses with negative-sense genomes. to determine the extent of homologous recombination in negative-sense rna viruses, phylogenetic analyses of 79 gene sequence alignments from 35 negative-sense rna viruses (a total of 2154 sequences) were carried out. powe ... | 2003 | 13679603 |
| [epidemic nephropathy in spain: first imported case]. | 2003 | 14642234 | |
| [typing of hemorrhagic fever with renal syndrome by cluster analysis]. | to make an inquiry into method of typing of hemorrhagic fever with renal syndrome (hfrs). | 2003 | 12975012 |
| [progress in the epidemiologic study of hemorrhagic fever with renal syndrome in china in recent years]. | 2003 | 15481132 | |
| [using recombinant antigens of hantavirus to study the kinetics of serum iga, igg, igm antibodies in the acute-phase of hemorrhagic fever renal syndrome]. | to observe the features of serum specific iga, igg, igm antibodies in the acute phase of hemorrhagic fever renal syndrome (hfrs). | 2003 | 15340571 |
| [expression of amino terminal of nucleoprotein and glycoprotein g2 of hantaan virus in insect cells in the form of fusion protein]. | to express the glycoprotein g2 and amino terminal of nucleoprotein (np) of hantaan virus in bac-to-bac baculovirus expression system in the form of fusion protein. | 2003 | 15155073 |
| expression of interferon inducible genes following hantaan virus infection as a mechanism of resistance in a549 cells. | hantaan virus (htn) is a causative agent of hemorrhagic fever with renal syndrome (hfrs). little is known of its pathogenesis or the molecular mechanisms underlying resistance to htn infection. in the present study, dna microarray technology was used to monitor changes in mrna levels after htn infection, to elucidate resistance mechanisms to viral infection by understanding virus-host interactions. we found that several interferon (ifn)-inducible genes were up-regulated in host cells infected wi ... | 2003 | 12680691 |
| mapping of the regions involved in homotypic interactions of tula hantavirus n protein. | hantavirus nucleocapsid (n) protein has been suggested to form homodimers and homotrimers that are further integrated into the nucleocapsid filaments around the viral rna. here we report detailed mapping of the regions involved in the homotypic n protein interactions in tula hantavirus (tulv). peptide scan screening was used to define the interaction regions, and the mammalian two-hybrid assay was used for the functional analysis of n protein mutants. to study linear regions responsible for n pr ... | 2003 | 14512541 |
| [surveillance on hemorrhagic fever with renal syndrome in guizhou during 1984-2000]. | to explore the relations between different areas on hemorrhagic fever with renal syndrome (hfrs) in guizhou. | 2003 | 14521790 |
| quality control measures for the serological diagnosis of hantavirus infections. | with society's rapidly increasing mobility, patients infected with severe viral infections can become seriously ill at any place in europe and elsewhere. improving the diagnostics of these infections is the most important step in detecting the pathogens and dealing with them, and for this purpose, quality control measures are essential tools. | 2003 | 14522063 |
| a system of protein target sequences for anti-rna-viral chemotherapy by a vitamin b6-derived zinc-chelating trioxa-adamantane-triol. | the synthesis of the structurally unusual heterotricyclic compound 1-[3-hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridinyl]-2,8,9-trioxaadamantane-3,5,7-triol (trivially named bananin, bn) from pyridoxylidenephloroglucinol and a theoretical prospect on possible biological activities of bn are presented in this report. pyridoxylidenephloroglucinol is synthesized by knoevenagel condensation of the vitamin b6 aldehyde pyridoxal with phloroglucinol. pyridoxylidenephloroglucinol rearranges to light-yello ... | 2003 | 14527557 |
| association of the nucleocapsid protein of the seoul and hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules. | we performed yeast two-hybrid screening of a human kidney cell cdna library to study the biological role of the hantavirus nucleocapsid protein (np). we found that seoul virus (seov) and hantaan virus (htnv) nps were associated with small ubiquitin-like modifier (sumo)-1-interacting proteins pias1, piasxbeta, hipk2, chd3, and ttrap, which interacted with the sumo-1 conjugating enzyme (ubc-9) and sumo-1 in the yeast two-hybrid assay. interactions between the hipk2, chd3, and ttrap proteins and se ... | 2003 | 14609633 |
| [study on the application of geographic information system in spatial distribution of hemorrhage fever with renal syndrome in china]. | to analyze the spatial distribution of hemorrhagic fever with renal syndrome (hfrs) in china by geographic information system, and to draw up a map on hrfs risk areas. | 2003 | 12820941 |
| prevalence of rabies virus and hantaan virus infections in commensal rodents and shrews trapped in bangkok. | five hundred rodents and shrews (rattus norvegicus: 458, rattus rattus: 28, rattus exulans: 5, mus musculus: 4 and suncus murine: 5) trapped from the fresh food markets around bangkok area were investigated for rabies virus and hantaan virus infections. no rabies viral antigens in the animals' brains were detected by direct immunofluorescence. on the other hand, antibodies to hantaan virus were demonstrated in the sera of 7 (1.53%) r. norvegicus caught in various markets using a particle aggluti ... | 2003 | 14696782 |
| the multimerization of hantavirus nucleocapsid protein depends on type-specific epitopes. | multimerization of the hantaan virus nucleocapsid protein (np) in hantaan virus-infected vero e6 cells was observed in a competitive enzyme-linked immunosorbent assay (elisa). recombinant and truncated nps of hantaan, seoul, and dobrava viruses lacking the n-terminal 49 amino acids were also detected as multimers. although truncated nps of hantaan virus lacking the n-terminal 154 amino acids existed as a monomer, those of seoul and dobrava formed multimers. the multimerized truncated np antigens ... | 2003 | 12502810 |
| diagnostic rapid tests for acute hantavirus infections: specific tests for hantaan, dobrava and puumala viruses versus a hantavirus combination test. | hantaviruses infecting humans in eurasia include hantaan, seoul, puumala and the closely related dobrava and saaremaa viruses. these viruses are causative agents of hemorrhagic fever with renal syndrome (hfrs), which is recognized as a severe health care problem in several countries. diagnostics of hantavirus infections relies on serology, performed principally with enzyme immunoassay (eia) or immunofluorescence assay (ifa). we developed four 5-min immunochromatographic igm-antibody tests for di ... | 2003 | 12565162 |
| use of vesicular stomatitis virus pseudotypes bearing hantaan or seoul virus envelope proteins in a rapid and safe neutralization test. | a vesicular stomatitis virus (vsv) pseudotype bearing hantavirus envelope glycoproteins was produced and used in a neutralization test as a substitute for native hantavirus. the recombinant vsv, in which the enveloped protein gene (g) was replaced by the green fluorescent protein gene and complemented with g protein expressed in trans (vsvdeltag*g), was kindly provided by m. a. whitt. 293t cells were transfected with plasmids for the expression of envelope glycoproteins (g1 and g2) of htnv or se ... | 2003 | 12522053 |
| ribavirin causes error catastrophe during hantaan virus replication. | except for ribavirin, no other antiviral drugs for treating hantaviral diseases have been identified. it is well established that ribavirin will inhibit the production of infectious hantaan virus (htnv); however, its mechanism of action is unknown. to characterize the inhibitory effect of ribavirin on htnv, the levels of viral rnas, proteins, and infectious particles were measured for 3 days posttreatment of htnv-infected vero e6 cells. htnv-infected cells treated with ribavirin showed a slight ... | 2003 | 12477853 |
| generation of an hfrs patient-derived neutralizing recombinant antibody to hantaan virus g1 protein and definition of the neutralizing domain. | hantaan virus (htnv) in the hantavirus genus, family bunyaviridae, is the major cause of severe hemorrhagic fever with renal syndrome (hfrs). we prepared a combinatorial phage display library of human fabs to htnv from rna extracted from the blood lymphocytes of a convalescent hfrs patient. we selected two g1 glycoprotein-specific clones and one nucleocapsid protein (n)-specific clone from the fab library for further studies. the human fab antibodies were converted to igg form in baculovirus/ins ... | 2003 | 12436484 |
| establishment of an enzyme-linked immunosorbent assay for detection of hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in escherichia coli. | a recombinant nucleocapsid protein of hantaan virus (htn) 76-118 strain expressed in e. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rhtn-elisa) for detection of hantavirus antibody in rat sera. the sensitivity and specificity of the rhtn-elisa were compared with those of the indirect immunofluoresent assay (ifa) using virus-infected cells. the sensitivity of rhtn-elisa was similar to that of the ifa both in experimentally sr-11 infected rat and naturally ... | 2003 | 12638233 |
| rescue of hantaan virus minigenomes. | hantavirus infections are a major public health concern worldwide. their widespread geographical distribution and their ability to produce serious, often fatal, human disease underline the need for a system that allows manipulation of these viruses. we describe here the first successful establishment of a reverse genetics technology for hantaan virus, the prototype of the genus hantavirus. the system offers a unique opportunity to study the biology of hantaviruses, the pathogenesis of the diseas ... | 2003 | 12642095 |
| hantaviruses: a tale of two hemispheres. | infectious febrile nephropathies, initially described as epidemics during military campaigns in europe and asia at the turn of the 20(th) century, were eventually all unified under the rubric of hemorrhagic fever with renal syndrome (hfrs) and ascribed to infection from rodent-borne hantaviruses. at the end of the century, hantavirus pulmonary syndrome (hps) was described throughout the americas as a consequence of infection with the new world hantaviruses. the epidemiology and clinical manifest ... | 2003 | 12682619 |