| characterization of neutralizing monoclonal antibody escape mutants of hantaan virus 76118. | neutralizing monoclonal antibody (mab) escape mutants of hantaan virus were generated using mabs to envelope protein g1 (16d2) and g2 (11e10). the mutant viruses (mu16d2 and mu11e10), lacked reactivity only to the selecting mab, or a mab belonging to the same antigenic site. both mutants had a single amino acid (a.a.) substitution. the a.a. substitution, found in mu16d2, was different from that found in another mutant selected with the same mab (16d2). although mab 11e10 immunoprecipitated g2 pr ... | 1998 | 9505967 |
| nucleotide sequence of nucleocapsid protein (n) of hantaan virus isolated from a korean hemorrhagic fever patient. | the nucleotide sequence of the nucleocapsid protein (n) coding region of a hantaan virus strain (cfc94-2) isolated from a korean hemorrhagic fever (khf) patient was determined by sequencing a series of deletion mutants. comparison of the n coding sequence of cfc94-2 to the sequence of the prototype of hantaan virus, strain 76-118 reveals a 4.97% difference in the nucleotide sequence and a 1.6% difference in the deduced amino acid sequence. the rate of amino acid sequence variation in n protein o ... | 1997 | 9524814 |
| [preventive effects of three kinds of inactive vaccines against epidemic hemorrhagic fever (ehf) after 5 years of vaccination]. | to observe the safety and the preventive effects of three kinds of vaccines as mongolian gerbils kidney vaccine, mouse brains vaccine and hamster kidney vaccine inoculated 5 years ago. | 2000 | 11860813 |
| [study on the genetic difference of seo type hantaviruses]. | to understand the genetic type of hantaviruses and the difference between them caused by rodents in beijing and to furhter explore the source of the infectious factors. | 2000 | 11860814 |
| [safety and immunogenicity of inactivated bivalent ehf vaccine in humans]. | safety and immunogenicity of inactivated bivalent ehf vaccine in humans were evaluated in the epidemic area of zhejiang province, china. | 2000 | 11860832 |
| [report on epidemic hemorrhagic fever (ehf) surveillance in 1999]. | to understand the epidemic tread of epidemic hemorrhagic fever (ehf) in 1999 and to develop programs for its control and prevention. | 2000 | 11860833 |
| serum and urine soluble hla class i antigen concentrations are increased in patients with hemorrhagic fever with renal syndrome. | in order to evaluate the association between the hantaan virus-induced cellular-immune response and clinical severity in patients with hemorrhagic fever with renal syndrome (hfrs). | 1997 | 9159038 |
| [experience in using regression analysis for assessing the quality of the diagnosis of hemorrhagic fever with renal syndrome]. | a new approach has been developed to evaluate the adequacy of hfrs clinical diagnosis, by using regression analysis of large selections. the hfrs morbidity rate and the antibody prevalence rate in man were viewed as a function and an argument of the function, respectively. an empiric model for one of the active hfrs endemic area (saratov province), characterizing the correlation between the immunity and morbidity rates has been elaborated. it calculates the theoretical morbidity level that corre ... | 1997 | 9182194 |
| hemorrhagic fever (puumala virus infection) with ocular involvement. | puumala virus infection (nephropathia epidemica) is a disease in the group of hemorrhagic fevers with renal syndrome causing ocular manifestations, e.g. transient myopia and changes in intraocular pressure. | 1998 | 9782434 |
| the rna binding domain of the hantaan virus n protein maps to a central, conserved region. | the nucleocapsid (n) protein of hantaviruses encapsidates both viral genomic and antigenomic rnas, although only the genomic viral rna (vrna) is packaged into virions. to define the domain within the hantaan virus (htnv) n protein that mediates these interactions, 14 n- and c-terminal deletion constructs were cloned into a bacterial expression vector, expressed, and purified to homogeneity. each protein was examined for its ability to bind the htnv s segment vrna with filter binding and gel elec ... | 2002 | 11884555 |
| transmission electron microscopic study of the hemorrhagic spots in patients with epidemic hemorrhagic fever in the early stage. | epidemic hemorrhagic fever (ehf) virus particles were found in the squamous epithelial cells and the capillary endothelial cells of the petechial spots located on the mucous membrane of the soft palate in 3 patients with severe early-stage ehf with transmission electron microscopy. the virus particles were round or oval in shape, about 100 nm in diameter, with a two-layer lipid envelope from which spikes were protruding. the nucleocapsid of the virus appeared to be hollow microfilamentoid or den ... | 1997 | 9183829 |
| transfection-mediated generation of functionally competent tula hantavirus with recombinant s rna segment. | since the discovery of rna recombination in polioviruses, there has been a general belief that this mechanism operates only in positive-sense rna viruses. recently, studying wild-type tula hantavirus, we observed a mosaic-like structure of the s rna segment that was consistent with generation by recombination between viruses from two genetic lineages. here we show transfection-mediated rescue of tula virus carrying recombinant s rna segment. independent attempts yielded s rna molecules of simila ... | 2002 | 11889055 |
| [high homology between antigen of hfrsv with anti-idiotype antibody against hfrsy]. | to detect the structural characterization of genes of the anti-idiotype antibody and the antigen. | 1996 | 9206197 |
| [serotyping of hemorrhagic fever with renal syndrome in hubei province]. | ninety-two serum specimens, positive for antibodies against hemorrhagic fever with renal syndrome (hfrs) virus in initial screening with immunofluorescence assay technic (ifat), were serotyped with micro cell pathogenic effects neutralization test based on preliminary epidemiological classification of epidemic foci of hfrs throughout the province to find out serological evidence of hfrs typing in hubei province. it was found that 48 of the specimens were belonged to type i (htn) accounting for 5 ... | 1997 | 9812603 |
| identification of a cell surface 30 kda protein as a candidate receptor for hantaan virus. | cellular receptors play an important role in virus pathogenesis. as a first step in virus infection, viruses attach to specific receptors on the surface of cells; hantaan virus infects susceptible cells by attaching to a receptor located on the cell surface. to date, the identity of the hantaan virus host cell receptor remains unknown. to determine the protein on the cell surface to which hantaan virus binds, a virus overlay protein-binding assay was performed with radiolabelled virus. a 30 kda ... | 2002 | 11907325 |
| [role of leptotrombidium (l.) scutellare in transmission of human diseases]. | a series of cases of hemorrhagic fever with renal syndrome (hfrs) in shaanxi province and autumn-type tsutsugamushi disease in jiangsu province were studied during 1988 to 1990 and 1986 to 1993 to explore the role of leptotrombidium (l.) scutellare in transmission of these diseases. results showed that l. (l.) scutellare is a dominant strain of mites inhabiting in rats and its seasonal fluctuation conformed to the seasonal distribution of these diseases. l. (l.) scutellare could naturally infect ... | 1996 | 9208519 |
| sonographic evaluation of gallbladder-wall thickening in hemorrhagic fever with renal syndrome: prediction of disease severity. | gallbladder-wall thickening (gbwt) frequently occurs in patients with hemorrhagic fever with renal syndrome (hfrs), an acute infectious disease caused by hantaviruses. hfrs is manifested by fever, hemorrhage, renal failure, and in many cases gastrointestinal symptoms, such as abdominal pain and tenderness. the clinical significance of gbwt in hfrs has not been reported. the purpose of this study was to investigate the incidence of gbwt and the relationship between gbwt and the severity of hfrs. | 2001 | 11486323 |
| [isolation, identification and certain characteristics of a hantaan virus proposed as candidate virus for killed vaccine]. | isolation and identification of a hantaan virus from hfrs patient's serum for killed vaccine development. | 2000 | 11503020 |
| hantavirus nucleocapsid protein coiled-coil domains. | the nucleocapsid (n) proteins of hantaviruses such as the sin nombre virus (snv) bind to membranes and viral rnas, associate with transcription and replication complexes, and oligomerize during the process of virus assembly. n proteins trimerize in vitro and in vivo, and associate via assembly domains at their amino- and carboxyl-terminal ends. because structure prediction algorithms suggested that n protein residues 3-75 form two coiled-coil motifs separated by an intervening kink or turn seque ... | 2002 | 12019266 |
| genetics of hantaviruses: implications to taxonomy. | hantaviruses (genus hantavirus, family bunyaviridae) represent a prime example of emerging viruses. since isolation of the prototype hantaan virus in the late 70s more than 20 new species have been described and the number is increasing fast thus demanding for a more refined classification. taking into account that hantaviruses are difficult to isolate in cell culture, one should not be surprised that most of the "newcomers" were first described as distinct hantavirus genotypes. moreover, the on ... | 2002 | 12038679 |
| [detection of hfrsv in eulaelaps shanghaiensis and ornithonyssus bacoti by using in situ hybridization]. | to provide molecular biological evidence of transmission of hemorrhogic fever with renal syndrome virus (hfrsv) by gamasid mites, eulaelaps shanghaiensis and ornithonyssus bacoti. | 1998 | 12078290 |
| hantaan virus infection causes an acute neurological disease that is fatal in adult laboratory mice. | hantaan virus, the etiological agent of korean hemorrhagic fever, is transmitted to humans from persistently infected mice (apodemus agrarius), which serve as the primary reservoir. here we demonstrate that several strains of adult mus musculus domesticus (c57bl/6, balb/c, akr/j, and sjl/j) were susceptible to hantaan virus infection when infected intraperitoneally. first clinical signs were loss of weight, ruffled fur, and reduced activity, which were followed by neurological symptoms, such as ... | 2002 | 12163608 |
| [epidemiologic and experimental studies on epidemic haemorrhagic fever virus in pigs]. | in order to study the roles of pigs with epidemic haemorrhagic fever virus, epidemiologic and experimental studies were carried out in pigs. aetiological, serological and polymerase chain reaction (pcr) techniques were performed. results showed that not only pigs could be infected with ehfv under natural or experimental conditions but susceptible to ehfv as well. virus may disseminate to many organs of the animals through blood and cause transient pathologic changes. ehfv duplicates inside the a ... | 1998 | 10322675 |
| golgi localization of hantaan virus glycoproteins requires coexpression of g1 and g2. | the membrane glycoproteins g1 and g2 of hantaan virus (htnv; family bunyaviridae) are encoded on the m rna genome segment as a precursor polypeptide that is cotranslationally cleaved by host proteases. g1 and g2 accumulate in the golgi complex in cells following either virus infection or transfection with m segment cdna. however, there are conflicting reports in the literature concerning the golgi targeting of separately expressed g1. to resolve these differences, a series of m segment and g1 co ... | 2002 | 12202203 |
| [bunyavirus--virus and host relationship: the coevolution between hantavirus and rodent]. | | 2002 | 12227181 |
| expression of icam-1 on the hantaan virus-infected human umbilical vein endothelial cells. | in hfrs, there is a varying degree of disseminated intravascular coagulation which was evident in the early phase of the illness. it is believed also that dic would be the consequence, at least in part, of functional changes of endothelium resulting in kinin activation and clinical syndrome. this study investigated the role of adhesion molecule in the pathogenesis of hantaan virus-related disease. | 1999 | 10461425 |
| murine leukemia virus pseudotypes of la crosse and hantaan bunyaviruses: a system for analysis of cell tropism. | we have developed a system for the preparation of la crosse virus (lac) and hantaan virus (htn) pseudotypes using a murine leukemia virus vector. after concentration, the pseudotypes were present in quantities sufficient to analyze cell tropism and neutralization. cells resistant to lac could not be infected with the mlv (lac) pseudotypes, and the pseudotypes were sensitive to neutralizing monoclonal antibodies prepared against lac glycoproteins, as well as to inhibition by a soluble form of the ... | 1999 | 10500280 |
| hantaan virus antibody prevalence in rodent populations of several provinces of northeastern thailand. | we conducted a serological survey of 632 rodents from the northeast region of thailand in order to assess the presence of hantaan-like viruses that may be a risk to the human population. rodents were collected from rice fields, houses and domestic gardens in five northeastern provinces and tested for igg reacting sera to hantaan antigen using enzyme-linked immunoassays. the overall prevalence of hantavirus infection in rodents was 2.1% (13/632). species that tested positive included bandicota in ... | 2002 | 12358618 |
| hantavirus infection of dendritic cells. | dendritic cells (dcs) play a pivotal role as antigen-presenting cells in the antiviral immune response. here we show that hantaan virus (htnv), which belongs to the bunyaviridae family (genus hantavirus) and causes hemorrhagic fever with renal syndrome, productively infects human dcs in vitro. in the course of htnv infection, dcs did not show any cytopathic effect and viral replication did not induce cell lysis or apoptosis. furthermore, htnv did not affect apoptosis-inducing signals that are im ... | 2002 | 12368315 |
| pathogenic and nonpathogenic hantaviruses differentially regulate endothelial cell responses. | hantaviruses cause two human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hantaviruses infect human endothelial cells but cause little or no damage to the infected endothelium. we analyzed with affymetrix dna arrays (santa clara, ca) the endothelial cell transcriptional responses directed by hantaviruses associated with hps [new york-1 virus (ny-1v)], hfrs [hantaan virus (htnv)], or by a hantavirus not associated with human disease [prospect hil ... | 2002 | 12368479 |
| the in vitro and in vivo protective activity of monoclonal antibodies directed against hantaan virus: potential application for immunotherapy and passive immunization. | hantaan virus (htnv), a member of the genus hantavirus, family bunyaviridae, is an etiologic agent causing a serious human disease, hemorrhagic fever with renal syndrome (hfrs), with a mortality as high as 15% and is also a potential bioterrorism agent. it is urgently needed to develop anti-htnv-neutralizing monoclonal antibodies (mabs) for treatment and prevention of htnv infection. in the present study, 18 murine mabs directed against htnv strain chen were generated and characterized. among th ... | 2002 | 12408987 |
| identification of h-2k(b)-restricted t-cell epitopes within the nucleocapsid protein of hantaan virus and establishment of cytotoxic t-cell clones. | although neutralizing antibodies against hantaan virus (htv) can protect hosts from viral infection, t-cell responses to htv are also important in host defense against htv. however, much less is known about cytotoxic t lymphocyte (ctl) responses to htv. to identify ctl epitopes in the htv nucleocapsid protein (np), we selected 7 h-2k(b)-motif-fitting peptides. of these peptides, 3 peptides (np3, np4, and np7) were recognized by ctl responses derived from htv-immunized mouse splenocytes. np3 and ... | 2000 | 10596020 |
| serological study of hantavirus in man in the autonomous community of madrid, spain. | data relating to hantavirus infection in spain are scarce and limited to rural areas. the aim of this work was to study the seroprevalence of hantavirus infection in the autonomous community of madrid (acm), a region containing both rural and urban populations in different ecological settings. sera from 3852 individuals (1849 male, 2003 female) were screened by indirect inmunofluorescence, with vero e6 cells infected with puumala, hantaan and seoul viruses as antigens. screen-positive results we ... | 2002 | 12435066 |
| generation of an hfrs patient-derived neutralizing recombinant antibody to hantaan virus g1 protein and definition of the neutralizing domain. | hantaan virus (htnv) in the hantavirus genus, family bunyaviridae, is the major cause of severe hemorrhagic fever with renal syndrome (hfrs). we prepared a combinatorial phage display library of human fabs to htnv from rna extracted from the blood lymphocytes of a convalescent hfrs patient. we selected two g1 glycoprotein-specific clones and one nucleocapsid protein (n)-specific clone from the fab library for further studies. the human fab antibodies were converted to igg form in baculovirus/ins ... | 2003 | 12436484 |
| phylogenetic evidence for host switching in the evolution of hantaviruses carried by apodemus mice. | phylogenetic analysis of three hantaviruses: hantaan (htnv), dobrava (dobv), and the newly designated serotype/genotype saaremaa (saav) and their respective hosts, rodents of genus apodemus, reveals a discrepancy in the virus-host relationships. while all apodemus agrarius sequences from europe and the far east are monophyletic, saav (carried by the western subspecies of a. agrarius) shared the most recent ancestor with a. flavicollis-associated dobv virus, but not with htnv (carried by the east ... | 2002 | 12457975 |
| ribavirin causes error catastrophe during hantaan virus replication. | except for ribavirin, no other antiviral drugs for treating hantaviral diseases have been identified. it is well established that ribavirin will inhibit the production of infectious hantaan virus (htnv); however, its mechanism of action is unknown. to characterize the inhibitory effect of ribavirin on htnv, the levels of viral rnas, proteins, and infectious particles were measured for 3 days posttreatment of htnv-infected vero e6 cells. htnv-infected cells treated with ribavirin showed a slight ... | 2003 | 12477853 |
| natural foci of some viral zoonoses in croatia. | results of several-year investigations into the epidemiology and virology of viral zoonoses with natural foci occurring in croatia, with special reference to tick-borne encephalitis (tbe) and hemorrhagic fever with renal syndrome (hfrs), are presented. the etiologic diagnosis of these diseases was made on the basis of virus isolation from patient blood and/or findings of specific antibodies in the serum. several tbe and bhanja virus strains were isolated from different species of ticks, and calo ... | 1999 | 10914135 |
| the multimerization of hantavirus nucleocapsid protein depends on type-specific epitopes. | multimerization of the hantaan virus nucleocapsid protein (np) in hantaan virus-infected vero e6 cells was observed in a competitive enzyme-linked immunosorbent assay (elisa). recombinant and truncated nps of hantaan, seoul, and dobrava viruses lacking the n-terminal 49 amino acids were also detected as multimers. although truncated nps of hantaan virus lacking the n-terminal 154 amino acids existed as a monomer, those of seoul and dobrava formed multimers. the multimerized truncated np antigens ... | 2003 | 12502810 |
| cis-acting signals in encapsidation of hantaan virus s-segment viral genomic rna by its n protein. | the nucleocapsid (n) protein encapsidates both viral genomic rna (vrna) and the antigenomic rna (crna), but not viral mrna. previous work has shown that the n protein has preference for vrna, and this suggested the possibility of a cis-acting signal that could be used to initiate encapsidation for the s segment. to map the cis-acting determinants, several deletion rna derivatives and synthetic oligoribonucleotides were constructed from the s segment of the hantaan virus (htnv) vrna. n protein-rn ... | 2001 | 11222687 |
| primary humoral immune responses to formalin inactivated hemorrhagic fever with renal syndrome vaccine (hantavax): consideration of active immunization in south korea. | the efficacy of a formalin-inactivated hemorrhagic fever with renal syndrome (hfrs) vaccine and the effectiveness of a related vaccination program have not been previously evaluated. we measured the primary immune responses to hantavax by plaque reduction neutralizing antibody test (prnt), hemagglutination inhibition test (hai), elisa and high density particle agglutination test (hdpa) in order to confirm a possible biological efficacy through independent substantiation of experimental results a ... | 2001 | 11456392 |
| use of vesicular stomatitis virus pseudotypes bearing hantaan or seoul virus envelope proteins in a rapid and safe neutralization test. | a vesicular stomatitis virus (vsv) pseudotype bearing hantavirus envelope glycoproteins was produced and used in a neutralization test as a substitute for native hantavirus. the recombinant vsv, in which the enveloped protein gene (g) was replaced by the green fluorescent protein gene and complemented with g protein expressed in trans (vsvdeltag*g), was kindly provided by m. a. whitt. 293t cells were transfected with plasmids for the expression of envelope glycoproteins (g1 and g2) of htnv or se ... | 2003 | 12522053 |
| [efficacy of amixine in experimental hantavirus infection]. | the experimental studies conducted on 2-week suckling mice infected with hantaan virus, strain 76-118) treated with oral and subcutaneous amoxine showed its prophylactic, therapeutical-and-prophylactic, and therapeutical efficiencies. oral amoxine exhibited the highest efficiency when used in a dose of 10 mg/kg-1 96 hours before infection and throughout the incubation period. the protective efficiency was 61%. subcutaneously, the agent was effective when three schemes for injection in a dose of ... | 2002 | 12522965 |
| diagnostic rapid tests for acute hantavirus infections: specific tests for hantaan, dobrava and puumala viruses versus a hantavirus combination test. | hantaviruses infecting humans in eurasia include hantaan, seoul, puumala and the closely related dobrava and saaremaa viruses. these viruses are causative agents of hemorrhagic fever with renal syndrome (hfrs), which is recognized as a severe health care problem in several countries. diagnostics of hantavirus infections relies on serology, performed principally with enzyme immunoassay (eia) or immunofluorescence assay (ifa). we developed four 5-min immunochromatographic igm-antibody tests for di ... | 2003 | 12565162 |
| [central hemodynamic disorders and rheological red blood cell properties in hemorrhagic fever patients with renal syndrome]. | the aim of this study was to detect a relationship between hemodynamic disorders in patients with hemorrhagic fever with the renal syndrome (hfrs) and erythrocyte aggregability and erythrocyte membrane atpase activity. a total of 100 patients with hfrs of different severity were examined. central hemodynamic parameters were studied: circulating blood volume, minute volume, cardiac index, stroke volume, and total peripheral vascular resistance during preoliguria, oliguria, and polyuria periods. b ... | 2001 | 11510183 |
| [seroepidemiologic study on hemorrhagic fever with renal syndrome virus in the migration areas of the three-gorges reservoir chongqing, region, yangtze river]. | to ascertain both the immune level and latent infection status among the migrants from the three-gorge reservoir, chongqing region. | 2001 | 11718068 |
| [experimental study on the roles of gasmid mite and chigger mite in the transmission of hemorrhagic fever with renal syndrome virus]. | to study the roles of gasmid mite and chigger mite in the transmission of hemorrhagic fever with renal syndrome virus (hfrsv). | 2001 | 11769690 |
| establishment of an enzyme-linked immunosorbent assay for detection of hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in escherichia coli. | a recombinant nucleocapsid protein of hantaan virus (htn) 76-118 strain expressed in e. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rhtn-elisa) for detection of hantavirus antibody in rat sera. the sensitivity and specificity of the rhtn-elisa were compared with those of the indirect immunofluoresent assay (ifa) using virus-infected cells. the sensitivity of rhtn-elisa was similar to that of the ifa both in experimentally sr-11 infected rat and naturally ... | 2003 | 12638233 |
| rescue of hantaan virus minigenomes. | hantavirus infections are a major public health concern worldwide. their widespread geographical distribution and their ability to produce serious, often fatal, human disease underline the need for a system that allows manipulation of these viruses. we describe here the first successful establishment of a reverse genetics technology for hantaan virus, the prototype of the genus hantavirus. the system offers a unique opportunity to study the biology of hantaviruses, the pathogenesis of the diseas ... | 2003 | 12642095 |
| increased expression of hsp70 and co-localization with nuclear protein in cells infected with the hantaan virus. | to investigate the effect of hantaan virus infection on the expression of stress genes. | 2001 | 11780421 |
| [clinical study on intrauterine hemorrhagic fever with renal syndrome virus infection]. | to find out the status of intrauterine infection with hemorrhagic fever with renal syndrome virus (hfrsv). | 2002 | 12665941 |
| [analysis of hfrs vaccination against hemorrhagic fever with renal syndrome during 1999-2000 in laiwu city]. | to evaluate the effect the vaccine of hemorrhagic fever with renal syndrome (hfrs). | 2002 | 12665942 |
| [a study on the epidemiology and preventive measure on epidemic haemorrhage fever in army barrack area in shandong]. | in order to guide the army and civilians to carry out the prevention on epidemic haemorrhage fever (ehf), it's necessary to elucidate the epidemic feature of natural epidemic foci and the risk factors of ehf in the army - stationed areas. | 2000 | 11860754 |
| hantaviruses: a tale of two hemispheres. | infectious febrile nephropathies, initially described as epidemics during military campaigns in europe and asia at the turn of the 20(th) century, were eventually all unified under the rubric of hemorrhagic fever with renal syndrome (hfrs) and ascribed to infection from rodent-borne hantaviruses. at the end of the century, hantavirus pulmonary syndrome (hps) was described throughout the americas as a consequence of infection with the new world hantaviruses. the epidemiology and clinical manifest ... | 2003 | 12682619 |
| human recombinant neutralizing antibodies against hantaan virus g2 protein. | old world hantaviruses, causing hemorrhagic fever with renal syndrome (hfrs), still present a public health problem in asia and eastern europe. the majority of cases has been recorded in china. the aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. to preserve the structural identity of viral protein, the panning procedure was performed on native hantaan (htn) (76-118) virus propagated in vero-e6 cells. in total, five complete ... | 2003 | 12706090 |
| [detection and proliferation of hemorrhagic fever virus in chigger mites]. | to study the impact of leptotromhidium scutellaris as a vector in transmission of hemorrhagic fever. | 1999 | 11864463 |
| [comparison of subclinical infection rates between vaccinated group with type i inactivated vaccine against hemorrhagic fever and controls]. | to compare subclinical infection rate in the vaccinated group with type i inactivated vaccine against hemorrhagic fever with renal syndrome (hfrs) with that in the controls and to understand its enhancement. | 1999 | 11864500 |
| [a study on the pathogenetic role of the viral structural proteins of hemorrhagic fever with renal syndrome]. | in order to clarify the pathogenesis of hemorrhagic fever with renal syndrome (hfrs), we detected hfrs virus structural proteins expressed in perepheral blood mononuclear cells (pbmc) by using immunohistochemical technique. meanwhile we also determined plasma endothelin (et), substance p (sp) and some sensitive markers of renal function with radioimmunoassay. the result showed that in hfrs patients, from the day of admission up to the thirteenth day of illness, the antigens of hfrs virus membran ... | 1996 | 9592317 |
| [an epidemic of hemorrhagic fever with renal syndrome in croatia in 1995]. | in the spring of 1995, the largest outbreak of hemorrhagic fever with renal syndrome (hfrs) so far was recorded in croatia. there were 125 patients reported to the national croatian institute of public health, department of epidemiology. the disease occurred simultaneously in several localities, some of them close to the previously known natural foci (mala kapela, western slavonia); the focus on dinara was newly discovered. war circumstances in croatia were closely related to this outbreak. ther ... | 1997 | 9658776 |
| evidence of the presence of seoul virus in cambodia. | a study was conducted in agricultural and urban areas in cambodia to assess the presence of hantaviruses in rodent populations. in 1998, rodents were trapped in two villages and in phnom penh city around market places and a rubbish dump. igg antibodies to hantaan virus were detected in 54 (8.2%) rodents among 660 tested: 6.4% (13/203) among roof rats (rattus rattus), 20.9% (39/187) among norway rats (r. norvegicus), 16.7% (2/12) among unidentified rattus species and none in 183 polynesian rats ( ... | 2003 | 12850202 |
| hantavirus-specific cd8(+)-t-cell responses in newborn mice persistently infected with hantaan virus. | the relationship between virus-specific cd8(+)-t-cell responses and viral persistence was studied in mice by using hantaan virus (htnv). we first established a simple method for measuring levels of virus-specific cd8(+) t cells by flow cytometry. next, to produce a mouse model of persistent htnv infection, newborn mice were inoculated subcutaneously within 24 h of birth with 1 or 0.1 50% newborn mouse lethal dose of htnv. all mice that escaped lethal infection were persistently infected with htn ... | 2003 | 12857910 |
| [detection of hantaan virus from gamasid mite and chigger mite by molecular biological methods]. | to study the proliferation and location of hantaan virus (hv) in gamasid mites and chigger mites. | 2003 | 12869987 |
| [molecular characterization of hantavirus shandong isolate jnl virus strain]. | to understand the molecular epidemiologic characteristics of hantavirus shandong isolate jnl virus strain. | 2003 | 12869988 |
| [construction and stable expression of intracellular antibodies to glycoprotein of hantavirus]. | to understand the molecular mechanisms of hantavirus assembly and maturation by stably expressing the intracellular antibodies to hantavirus glycoprotein g1 and g2 in endoplasmic reticulum (er) and cytoplasm (cyto) of vero e6 cell. | 2003 | 12869989 |
| [molecular analysis of hantavirus isolated from shandong province]. | to compare the molecular characteristics of hantaviruses isolated from shandong province by using pcr typing and nucleotide sequencing. | 2003 | 12869990 |
| [serum antibody response and hantavirus rna sequencing in patients with hemorrhagic fever renal syndrome in yantai areas]. | to understand antibody responses to and rna sequences of hantavirus in patients with hemorrhagic fever renal syndrome (hfrs) in yantai areas and to demonstrate the type of the prevalent viruses caused hfrs. | 2003 | 12869991 |
| [passage adaptability of candidate strains for hemorrhagic fever with renal syndrome purified vaccine in vero cells and their immunogenicity]. | to adapt the candidate strains of hemorrhagic fever with renal syndrome (hfrs) purified vaccine to vero cells and to study their antigenicity and immunogenicity. | 2003 | 12869992 |
| [a study on immunogenicity and safety of bivalent inactivated vaccine against hemorrhagic fever with renal syndrome]. | to study side effects and effectiveness of bivalent inactivated vaccine against hemorrhagic fever with renal syndrome in population of a randomized controlled field trial. | 1999 | 11864504 |
| [antigenic and genetic properties of seoul virus strains--a causative agent of hemorrhagic fever with renal syndrome]. | data on the circulation of a new genetic variant of seol virus, which was designated as "vladivostok" (the name of the place where it was isolated)--vdv, were obtained, by using the antigenic and genetic analysis of hantavirus strains isolated from patients with hemorrhagic fever with renal syndrome (hfrs) and from brown rats, and described in the present paper. a complete identity of nucleotide sequences of the m-segment fragment of isolates obtained from hfrs patients and of those obtained fro ... | 2003 | 12894473 |
| serological analysis of hemorrhagic fever with renal syndrome (hfrs) patients in far eastern russia and identification of the causative hantavirus genotype. | hemorrhagic fever with renal syndrome (hfrs) is endemic in east asia and europe. the disease is caused by several viruses belonging to the genus hantavirus, including the hantaan virus (htnv), seoul virus (seov), dobrava belgrade virus (dobv), and puumala virus (puuv). recently, htnv-related viruses, amur (amr) and far east (fe) genotypes were identified as causative agents of hfrs in far eastern russia. to investigate the epidemiology of hfrs and virus transmission, we collected sera from 17 ac ... | 2003 | 12898330 |
| hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis. | the cellular entry of hantaan virus (htn) occurs through interactions with beta(3) integrins as cellular receptors. however, the process of htn infection following attachment to the cell surface is not well understood. our data indicate that overexpression of a dominant-negative mutant dynamin inhibits htn internalization and that compounds that block clathrin- but not caveolae-dependent endocytosis also reduce htn infectivity. in addition, we show that htn colocalizes with the clathrin heavy ch ... | 2002 | 11886265 |
| hemorrhagic fever with renal syndrome presenting with hemophagocytic lymphohistiocytosis. | hemophagocytic lymphohistiocytosis--which is associated with a variety of infections, malignant neoplasms, autoimmune diseases, and immunodeficiencies--is an uncommon syndrome with a rapidly fatal outcome. we describe the first case of hemorrhagic fever with renal syndrome due to hantaan virus presenting with reactive hemophagocytosis. | 2002 | 11897077 |
| serologic diagnosis of hantaan virus infection based on a peptide antigen. | | 2002 | 11901064 |
| mapping of the regions involved in homotypic interactions of tula hantavirus n protein. | hantavirus nucleocapsid (n) protein has been suggested to form homodimers and homotrimers that are further integrated into the nucleocapsid filaments around the viral rna. here we report detailed mapping of the regions involved in the homotypic n protein interactions in tula hantavirus (tulv). peptide scan screening was used to define the interaction regions, and the mammalian two-hybrid assay was used for the functional analysis of n protein mutants. to study linear regions responsible for n pr ... | 2003 | 14512541 |
| [the therapeutic effect of purified human leucocytic interferon-alpha on hemorrhagic fever with renal syndrome]. | in order to know the therapeutic effect of purified human leucocytic interferon-alpha on hemorrhagic fever with renal syndrome diagnosed early (< or = 5 days). we treated 23 cases with clinical prognosis and antiviral effect significantly better than those of 21 cases in a random control group. the membrane protein (mp), nucleo protein (np) in peripheral blood mononuclear cells (pbmc) and urine, the serum antibodies to mp and np were determined before treatment and 2, 4, 6, 8, 10 days after the ... | 1997 | 9812566 |
| quality control measures for the serological diagnosis of hantavirus infections. | with society's rapidly increasing mobility, patients infected with severe viral infections can become seriously ill at any place in europe and elsewhere. improving the diagnostics of these infections is the most important step in detecting the pathogens and dealing with them, and for this purpose, quality control measures are essential tools. | 2003 | 14522063 |
| [a preliminary genetic reassortment between hantaan virus and seoul virus strains]. | to determine the frequency and characteristics of reassortment among hantaan and seoul viruses causing hemorrhagic fever with renal syndrome (hfrs). | 2002 | 12015110 |
| association of the nucleocapsid protein of the seoul and hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules. | we performed yeast two-hybrid screening of a human kidney cell cdna library to study the biological role of the hantavirus nucleocapsid protein (np). we found that seoul virus (seov) and hantaan virus (htnv) nps were associated with small ubiquitin-like modifier (sumo)-1-interacting proteins pias1, piasxbeta, hipk2, chd3, and ttrap, which interacted with the sumo-1 conjugating enzyme (ubc-9) and sumo-1 in the yeast two-hybrid assay. interactions between the hipk2, chd3, and ttrap proteins and se ... | 2003 | 14609633 |
| [complete genome sequence of 84fli, a hantaan virus strain isolated from the liver of fetus aborted by a pregnant women with hemmorrhagic fever with renal syndrome]. | to study the complete genome sequence of the chinese hantaan virus vaccine strain 84fli and learn about its molecular characters. | 2002 | 12181094 |
| prevalence of rabies virus and hantaan virus infections in commensal rodents and shrews trapped in bangkok. | five hundred rodents and shrews (rattus norvegicus: 458, rattus rattus: 28, rattus exulans: 5, mus musculus: 4 and suncus murine: 5) trapped from the fresh food markets around bangkok area were investigated for rabies virus and hantaan virus infections. no rabies viral antigens in the animals' brains were detected by direct immunofluorescence. on the other hand, antibodies to hantaan virus were demonstrated in the sera of 7 (1.53%) r. norvegicus caught in various markets using a particle aggluti ... | 2003 | 14696782 |
| microarray technology for identification and distinction of hantaviruses. | dna microarrays combine high-precision technology with advanced molecular biology to achieve high-throughput screening of dna fragments. in this study, we investigated the potential of the cdna microarray technique to identify and discriminate pcr derived amplicons from genetically highly similar viruses. the wide range of sequence variation among hantaviruses makes them suitable as a model for this purpose. the hantaviruses, carried by rodents, cause several hundred thousand cases of severe hum ... | 2004 | 14981768 |
| bovine aortic endothelial cells are susceptible to hantaan virus infection. | hantavirus serotype hantaan (htn) is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs, lethality up to 10%). the natural host of htn is apodemus agrarius. recent studies have shown that domestic animals like cattle are sporadically seropositive for hantaviruses. in the present study, the susceptibility of bovine aortic endothelial cells (baec) expressing alpha(v)beta(3)-integrin to a htn infection was investigated. viral nucleocapsid protein and genomic rna segments wer ... | 2004 | 15033559 |
| haemorrhagic fever with renal syndrome in croatia. | | 2002 | 12241810 |
| prevalence of hantavirus antibody in patients with chronic renal disease in egypt. | in egypt, the etiology of chronic renal failure (crf) is not well defined. a hospital-based case-control study was initiated in february 1998, to determine whether hantavirus infection is involved in chronic renal disease (crd) in egypt. the study enrolled 350 study patients with a history of crf and 695 matched controls with crd due to renal calculus or renal cancer, but with normal renal functions. sera from cases and controls were tested for anti-hantavirus igg using elisa with a cell-lysate ... | 2004 | 15099987 |
| [laboratory diagnosis of hemorrhagic fever with renal syndrome in the ulianovsk region]. | specific laboratory diagnosis is of great importance for the diagnosis of hemorrhagic fever with renal syndrome (hfrs). the results of the investigation confirm the persistence of the specific antibodies in blood sera of the patients with hfrs. the indices of the serologic confirmation of the diagnosis of hfns are proven to elevate if the optimal intervals of sampling the pair blood sera is observed. | 2002 | 12271728 |
| [distribution of hemorrhagic fever with renal syndrome virus in gamasid mites and chigger mites]. | to study the distribution of hemorrhagic fever with renal syndrome virus (hfrsv) in mites. | 2002 | 12411201 |
| [expression of amino terminal of nucleoprotein and glycoprotein g2 of hantaan virus in insect cells in the form of fusion protein]. | to express the glycoprotein g2 and amino terminal of nucleoprotein (np) of hantaan virus in bac-to-bac baculovirus expression system in the form of fusion protein. | 2003 | 15155073 |
| differential antiviral response of endothelial cells after infection with pathogenic and nonpathogenic hantaviruses. | hantaviruses represent important human pathogens and can induce hemorrhagic fever with renal syndrome (hfrs), which is characterized by endothelial dysfunction. both pathogenic and nonpathogenic hantaviruses replicate without causing any apparent cytopathic effect, suggesting that immunopathological mechanisms play an important role in pathogenesis. we compared the antiviral responses triggered by hantaan virus (htnv), a pathogenic hantavirus associated with hfrs, and tula virus (tulv), a rather ... | 2004 | 15163707 |
| characterization of hla-a2.1-restricted epitopes, conserved in both hantaan and sin nombre viruses, in hantaan virus-infected patients. | nine different ctl epitopes, conserved in both hantaan virus (htnv) and sin nombre virus (snv), were selected for study. the binding affinity of each peptide with hla-a2.1 molecules in vitro was determined and antigen-specific responses from seven donors who had a previous field infection with htnv were examined. although the strength or frequency of ctl activity showed different patterns in the seven patients, five of seven patients showed significant activity against at least one or more epito ... | 2002 | 11961268 |
| [comparative study on antibody levels of vaccination group and control group after 4 years of immunized with type b inactivated vaccine against hemorrhagic fever with renal syndrome]. | to compare the antibody level of vaccine group and control group in a randomized, controlled trial of type b inactivated vaccine against hemorrhagic fever with renal syndrome(hfrs) in jiande county of zhejiang province and to find out if there is enhancement of subclinical infection about type b inactivated vaccine against hfrs. | 2001 | 11986705 |
| [discovery of two sub-types of hantanvirus in anhui mountain areas]. | to investigate the existence of hemorrhagic fever with renal syndrome (hfrs) foci in high mountains and forest areas. | 2002 | 12482368 |
| [anthropogenic transformation of the environment and morbidity of infections with natural focality in the ul'ianovsk region]. | | 2002 | 12506644 |
| [proliferation and location of hantaan virus in gamasid mites and chigger mites, a molecular biological study]. | to study the proliferation and location of hantaan virus (hv) in gamasid mites and chigger mites and the significance of gamasid mites and chigger mites as vectors of transmission of hemorrhagic fever with renal syndrome (hfrs). | 2002 | 12509926 |
| the intracellular association of the nucleocapsid protein (np) of hantaan virus (htnv) with small ubiquitin-like modifier-1 (sumo-1) conjugating enzyme 9 (ubc9). | small ubiquitin-like modifier-1 (sumo-1) conjugating enzyme 9 (ubc9) conjugates sumo-1 to target proteins and modulates cellular processes such as signal transduction, transcription regulation, and cell growth regulation. we demonstrated here that the nucleocapsid protein (np) of hantaan virus (htnv) was associated with ubc9 and sumo-1 in vivo. analysis of the interaction between the truncated nps and ubc9 revealed that the amino acid residues at the positions between 101 and 238 in the np were ... | 2003 | 12573574 |
| [study on using colloidal gold immuno-dot assay to detect special antibody of hemorrhagic fever renal syndrome]. | to seek for a simple, sensitive and rapid assay to detect specific antibody of patients suffering from hemorrhagic fever renal syndrome (hfrs). | 2001 | 12575393 |
| application of cdna microarray technique to detection of gene expression in host cells infected with viruses. | cdna microarray technique was used to monitor changes in mrna levels in cells after hantaan virus (htnv) infection. the values of the ratio of medians for htnv and japanese encephalitis virus (jev) at the early stage of infection were compared and found similar, suggesting that the same or similar genes are associated with the early events of infection with either virus. the reproducibility of values of the "ratio of medians" for htnv was examined. we found that applying cluster analysis to the ... | 2002 | 12580375 |
| [purification and identification of recombinant nuclear protein of hantaan virus]. | to purify recombinant nuclear protein of hantaan virus. | 2002 | 12665907 |
| expression of interferon inducible genes following hantaan virus infection as a mechanism of resistance in a549 cells. | hantaan virus (htn) is a causative agent of hemorrhagic fever with renal syndrome (hfrs). little is known of its pathogenesis or the molecular mechanisms underlying resistance to htn infection. in the present study, dna microarray technology was used to monitor changes in mrna levels after htn infection, to elucidate resistance mechanisms to viral infection by understanding virus-host interactions. we found that several interferon (ifn)-inducible genes were up-regulated in host cells infected wi ... | 2003 | 12680691 |
| inactivated hantaan virus vaccine derived from suspension culture of vero cells. | we have developed a cell culture-derived, inactivated vaccine against hantaan virus for prevention of the hemorrhagic fever with renal syndrome (hfrs). hantaan virus was purified from a microcarrier culture of vero e6 cells by ultrafiltration and density gradient centrifugation. viral infection was inactivated by treatment of the viral stock with formaldehyde. immunogenic properties of the vaccine were characterized in comparison with hantavax, a mouse brain-derived, formalin-inactivated vaccine ... | 2003 | 12706671 |
| [study on the application of geographic information system in spatial distribution of hemorrhage fever with renal syndrome in china]. | to analyze the spatial distribution of hemorrhagic fever with renal syndrome (hfrs) in china by geographic information system, and to draw up a map on hrfs risk areas. | 2003 | 12820941 |
| relationship between the clinical features and the viral antigen in the extremity blood of the patients with hemorrhagic fever with renal syndrome. | the direct immunogold silver staining (d-igss) method was used to detect the viral antigen in the extremity blood of 67 cases of hemorrhagic fever with renal syndrome. the positive rate of viral antigen was the highest during the fever, hypotension and oligouria phrase; and the rate dropped gradually during the polyuria and convalescent phase. it is suggested that clinical staging was positively related with the percentage of the viral antigen positive cells (p < 0.001). it is concluded that the ... | 1999 | 12840882 |