| strand-specific detection of hantaan virus rna sequences by in vitro dna amplification. | hantaan virus often causes a fatal human disease, hemorrhagic fever with renal syndrome (hfrs). an assay for strand-specific detection and quantitation of hantaan virus rna was developed based on the polymerase chain reaction (pcr). a protocol for the efficient detection of both genomic rna and its transcript is presented, in which a reverse transcriptase reaction (rtr) followed by pcr with two common primers was used to distinguish between positive- and negative-stranded rna. using this method, ... | 1994 | 7898391 |
| hantaan and puumala virus antibodies in blood donors in samara, an hfrs-endemic region in european russia. | | 1996 | 8676649 |
| an immune adherence assay for discrimination between etiologic agents of hemorrhagic fever with renal syndrome. | antigens of the viruses that cause hemorrhagic fever with renal syndrome (hfrs) and antibodies to these antigens were titrated in an immune adherence hemagglutination (iaha) test and a cf test. vero-e6 cells infected with the sr-11 strain of hfrs virus, an isolate from rats associated with a laboratory outbreak in japan, and the 76-118 strain of hantaan virus, the etiologic agent of korean hemorrhagic fever, were used as antigens in the iaha, cf, and indirect immunofluorescent antibody (ifa) tes ... | 1984 | 6141206 |
| genetic and phylogenetic analyses of hantaviral sequences amplified from archival tissues of deer mice (peromyscus maniculatus nubiterrae) captured in the eastern united states. | the s and m segments of a hantavirus, enzymatically amplified from tissues of cloudland deer mice (peromyscus maniculatus nubiterrae) captured during 1985 in west virginia, diverged from strains of four corners virus from the southwestern united states by more than 16% and 6% at the nucleotide and amino acid levels, respectively. phylogenetic analysis suggested that this virus strain (designated monongahela) forms a possible evolutionary link between the four corners and new york hantaviruses. | 1996 | 8678841 |
| [observational and experimental studies on relationship between domestic pigs and epidemic hemorrhagic fever investigation]. | observational and experimental studies on the relationship between domestic pigs and epidemic hemorrhagic fever (ehf) were carried out during 1986 to 1994 with reverse passive hemagglutination (rpha), reverse passive hemagglutination inhibition (rphi), horse-radish peroxidase-staphylococcal protein a (hrp-spa), indirect immunofluorescence assay (ifa) and reverse transcript polymerase chain reaction (rt-pcr) to understand the roles of domestic pigs played in the epidemic areas. results showed dom ... | 1995 | 8697938 |
| serological evidence of human hantavirus infection in argentina, bolivia and uruguay. | a serological survey was conducted in 1985-1987 to determine the presence of infection for hantavirus in the general population in argentina, uruguay, paraguay and bolivia, as well as among rodent-exposed laboratory workers in argentina. out of 748 individuals tested by immunofluorescence 20 proved positive for hantaan virus 76/118 strain of whom 16 also reacted against seoul virus 80/39 strain and 2 against puumala virus sotkamo strain. ten out of 72 argentine laboratory workers were positive f ... | 1996 | 8734925 |
| production of recombinant hantavirus nucleocapsid protein expressed in silkworm larvae and its use as a diagnostic antigen in detecting antibodies in serum from infected rats. | the recombinant nucleocapsid protein (rnp) of hantaan virus was expressed by a baculovirus vector in silkworm hemolymph and was used as an antigen in western blotting (wb). the rnp is expressed in insoluble form in hemolymph; therefore simple washing of the insoluble fraction with phosphate-buffered saline by low-speed centrifugation allowed preparation of purified antigen for wb. the rnp had strain-specific and hantavirus-common epitopes similar to the authentic np antigen of hantavirus and was ... | 1995 | 8746523 |
| comments on some new viruses associated with old diseases in china. | | 1996 | 8758348 |
| disseminated intravascular coagulation in korean hemorrhagic fever. | to investigate the nature and role of coagulation and complement alterations in the pathogenesis of korean hemorrhagic fever (khf), the profiles from the early stages in 27 male patients were serially evaluated. evidence of disseminated intravascular coagulation (dic) was observed in 14 of the 27 patients (51.8%) sometime during the course of the disease. the earlier the coagulation tests were performed, the more frequently the evidence of dic was found. the mean serum c3 concentration was signi ... | 1983 | 6141786 |
| hemorrhagic fever with renal syndrome: first imported case of hantavirus infection in taiwan. | a patient infected with hantavirus, with resultant hemorrhagic fever with renal syndrome (hfrs), is reported. the patient was a 38-year-old man, living in tainan, taiwan, who had been visiting mainland china for 3 months when he suddenly developed fever and chills, generalized myalgia, abdominal pain and petechiae on his chest. he sought treatment in mainland china. his clinical course progressed through febrile, hypotensive, oliguria and polyuria phases. supportive care included one course of h ... | 1996 | 8772056 |
| genomic characterization of m and s rna segments of hantaviruses isolated from bats. | genomic analysis of three hantaan-like virus isolates from bats was performed. cleavage patterns of reverse transcription (rt)-polymerase chain reaction (pcr) products and nucleotide sequences of g2 region of m rna segment and n protein region of s rna segment of the isolates were compared to that of hantaan 76-118 strain. genomic characteristics of the bat isolates were identical to that of hantaan virus. | 1995 | 8825306 |
| acute renal failure due to hemorrhagic fever with renal syndrome. | the aim of our study was to analyze the clinical course and outcome of acute renal failure (arf) in patients with hemorrhagic fever with renal syndrome (hfrs). from 1983 to 1995, we treated 33 patients (27 males, 6 females) aged from 16 to 71 years. half of patients were connected with work at a farm or in a forest. the disease was confirmed serologically with indirect immunofluorescence test (ift) and enzyme-linked immunosorbent assay (elisa). in 18 patients percutaneous kidney needle biopsies ... | 1996 | 8875690 |
| immune responses to inactivated vaccine in people naturally infected with hantaviruses. | an inactivated hantaan virus vaccine for hemorrhagic fever with renal syndrome (hfrs) was given by injection to 15 people who were naturally infected with either hantaan or seoul viruses. immunofluorescent antibody (ifa), reversed passive hemagglutination inhibition (rphi), hemagglutination inhibition (hi), and neutralization antibody (na) assays were used to measure the antibody titers of the vaccinated people before and after three doses of vaccine. the results indicated that ifa and rphi anti ... | 1996 | 8877768 |
| immunohistochemical detection of hantaan virus antigen in renal tissue from patient with hemorrhagic fever with renal syndrome. | | 1994 | 7915405 |
| seroepidemiology of hantaan virus infection in taiwan. | in order to investigate the infection rate of hantaan virus in taiwan, a total of 6,536 human serum samples were collected from residents, selected by stratified random sampling, from 19 townships covering four different ethnic groups: aborigines, fukien taiwanese, hakka taiwanese, and mainland chinese. serum samples were screened for hantaan virus antibodies by indirect immunofluorescence. the prototype hantaan virus (76/118)-infected vero e6 cells were used as the viral antigen for the antibod ... | 1996 | 8950687 |
| [hantavirus-induced acute renal failure]. | | 1996 | 9036104 |
| [the detection of antibodies to the hantaan virus in the urine of patients with hemorrhagic fever with renal syndrome]. | using a commercial diagnostic preparation for indirect ifa test, 135 urine samples from 50 patients with hfrs were examined at different periods of the disease. antibodies were demonstrable in all urine specimens from hfrs patients for 13 days. in 14-20 days they could be detected in half of the patients, and no antibodies could be demonstrated since the 21st day on. the results of urine examination from healthy subjects and some patients with other clinical diagnoses were negative same as contr ... | 1993 | 7915448 |
| investigation on inactivated epidemic hemorrhagic fever tissue culture vaccine in humans. | from 1988 to 1992, 2479 volunteers were immunized by inactivated meriones unguiculatus kidney cell (mukc) vaccine. their seroconversion rates of neutralizing antibody were about 90% after 3 doses of the vaccine. no side effects were found. the vaccine stored for 14 months at 4 degrees c still had good immunogenicity. it was shown that the vaccine was safe, effective and stable. detailed observation was made in 75 volunteers. the results showed that the seroconversion rate by plaque reduction neu ... | 1994 | 7916278 |
| the fourth china national congress on medical virology. | | 1994 | 7924587 |
| [pathological-anatomical findings after serologic and molecular biologic evidence of hantaan virus infection]. | post-mortem findings in a girl aged 2 years and 4 months who had been infected with hantaan virus are reported. the child fell ill with acute renal failure in her 8th month of life and had to be subjected to chronic dialysis. there was a progressive cardiac insufficiency. specific igg and igm antibodies to hantaan virus were demonstrated by serology from the 18th month of life. autopsy revealed endomyocardial fibrosis and cardiomegaly. hantaan rna could be demonstrated in formalin-fixed, paraffi ... | 1994 | 7947624 |
| characteristics of immunocomplex in autopsy tissues of hemorrhagic fever with renal syndrome. | using repeated pap or repeated pap and abc immunocytochemical methods, we were able to demonstrate viral antigens, ig and clq in the tissues of 20 autopsy materials that had been preserved for 3-30 years. serial paraffin sections were stained with the first antibodies against both viral antigens (g2 and np) and human igg, igm, c3 and clq. immunocomplex, composed of viral antigen, igg and clq were found diffusely in the cells of each organ and extracellularly in the sera, various secretions and e ... | 1994 | 7956485 |
| a major antigenic domain of hantaviruses is located on the aminoproximal site of the viral nucleocapsid protein. | hantavirus nucleocapsid protein has recently been shown to be an immunodominant antigen in hemorrhagic with renal syndrome (hfrs) inducing an early and long-lasting immune response. recombinant proteins representing various regions of the nucleocapsid proteins as well as segments of the g1 and the g2 glycoproteins of hantavirus strains cg18-20 (puumala serotype) and hantaan 76-118 have been expressed in e. coli. the antigenicity of these proteins was tested in enzyme immunoassays and immunoblots ... | 1997 | 9208453 |
| domestic cases of hemorrhagic fever with renal syndrome in the united states. | although serologic studies have identified hantaviral infection in the united states, acute disease has not been recognized. this study describes 3 cases of domestically acquired hemorrhagic fever with renal syndrome (hfrs) in the united states. infection was due to a local strain of seoul virus (baltimore rat virus). a review of the clinical features indicated a mild illness characterized by nausea, vomiting, renal and liver failure similar to hfrs described elsewhere for rat-borne viruses. fol ... | 1994 | 7991040 |
| puumala type hantavirus infection with early choleriform syndrome and severe multiorgan failure. | | 1997 | 9255656 |
| high prevalence of hantavirus infection in a group of chinese patients with acute hepatitis of unknown aetiology. | in southwestern china, small but substantial numbers of patients with acute hepatitis were found without known hepatropic viral infections (hepatitis a, b, c, d or e, cytomegalovirus, epstein-barr virus) and were receiving no hepatotoxic drugs. prevalence of antibodies, both neutralizing and specific immunoglobulin (ig)m and igg, to hantaan virus were evaluated in a cohort of 136 such patients: 83 were of unknown aetiology, 53 had known viral hepatitis and 59 healthy subjects acted as controls. ... | 1997 | 9278220 |
| a severe case of hemorrhagic fever with renal syndrome in singapore. | the incidence of human hantavirus infection in singapore is low. hitherto, only 2 cases of hemorrhagic fever with renal syndrome (hfrs) have been reported. a third case is reported here of a ragman who had the classical manifestations of hfrs, confirmed by serology. in addition, his condition was complicated by liver dysfunction and neurological disturbance. after a protracted stay, the patient was finally discharged from hospital 5 months after the onset of his severe illness. | 1996 | 9280013 |
| puumala and dobrava viruses cause hemorrhagic fever with renal syndrome in bosnia-herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera. | hantavirus infection was diagnosed serologically by mu-capture igm and igg elisas in hemorrhagic fever with renal syndrome (hfrs) patients admitted to tuzla hospital, bosnia-herzegovina. the results indicated that more than one hantavirus caused the outbreak. to address the question of which hantavirus serotypes were involved, sequentially drawn sera were analyzed by focus reduction neutralization test (frnt) for antibodies against puumala, hantaan, dobrava, and seoul hantaviruses. the data reve ... | 1997 | 9298732 |
| [the ecological aspects of the epidemiology of hemorrhagic fever with renal syndrome in the south of the far east in russia]. | the data on the relationship between the epizootic process in the population of mice (the source of hantavirus infection) and the seasonal manifestations of morbidity in hemorrhagic fever with renal syndrome (hfrs) in the southern region of the russian far east are presented. the asynchronous course of the epizootic process in the population of rodents causes irregularity in the spread of hfrs in the region and periodicity in the appearance of foci. seasonal rises in hfrs morbidity coincide with ... | 1997 | 9304320 |
| epitope mapping by phage display: random versus gene-fragment libraries. | we present a comparative study on epitope mapping of four monoclonal antibodies directed against four different antigens using alternative phage display techniques and peptide scanning: mab215 reacts with the largest subunit of rna polymerase ii, mabbp53-11 with the tumor suppressor protein p53, mabgdo5 with the hantaan virus glycoprotein g2 and mabl13f3 with the hantaan virus nucleocapsid protein. epitopes were determined (i) by gene-fragment phage display libraries, constructed by dnasei diges ... | 1997 | 9328567 |
| expression strategy of the m genome segment of hantaan virus. | the medium (m) genome segment of hantaan virus encodes the envelope glycoproteins, g1 and g2, in a continuous open reading frame with a gene order of 5'-g1-g2-3' with respect to the virus-complementary sense rna. because potential translation initiation codons and amino acids constituting typical signal sequences precede both the g1 and g2 genes, we sought to determine if g1 and g2 can be expressed independently. to investigate translational requirements for g1 and g2, we constructed m segment g ... | 1994 | 8165863 |
| hantavirus infection in scid mice. | severe combined immunodeficiency (scid) mice were inoculated with hantaan virus strain 76-118 (htn) or seoul virus strain sr-11 (sr) of hantaviruses. susceptibility of scid mice was compared with those of immunocompetent adult mice, newborn mice and nude mice. scid mice inoculated with htn or sr died 32 to 35 days after infection. unlike newborn mice which also died of hantavirus infection, scid mice survived longer than newborn mice and showed typical wasting symptoms rather than nervous sympto ... | 1997 | 9362032 |
| [study on the mode of transmission of ehfv among host animals and its epidemiologic significance]. | in our study, ifa was adopted to detect the epidemic haemorrhagic fever virus (ehfv) antigen and antibody. we studied on the mode of transmission of ehfv in mice and its epidemiologic significance. the results demonstrated that both horizontal and vertical modes of transmission existed in mice under natural circumstances. horizontal transmission was noticed as the major mode of transmission of ehfv in mice which played an important role in the spread and development of ehf natural foci. vertical ... | 1996 | 9387588 |
| high frequency of antibodies to hantaan virus and hepatitis c virus in chronic haemodialysis patients. coincidence or cross-reaction? | to address the question of whether there is any coincidence or cross-reaction between hepatitis c virus (hcv) and hantaan virus (both rna arboviruses), as well as to assess the frequency of antibodies to the above viruses amongst chronic haemodialysis patients in our region. | 1993 | 8258753 |
| [the unmasking of secondary adrenal insufficiency and central diabetes insipidus by a highly febrile acute hantaan virus infection]. | case report: a 32-year-old obese patient was admitted to our hospital as an emergency with very severe abdominal pain. routine examinations initially failed to establish a diagnosis. the patient then went on to develop a high fever that was associated with polydipsia and polyuria. serological studies revealed an acute infection with the hantaan virus. laboratory investigations showed hypofunction of the anterior and posterior lobes of the pituitary, with complete absence of acth, which was cause ... | 1997 | 9483917 |
| serological relationships among viruses in the hantavirus genus, family bunyaviridae. | we examined the serological relationships among 32 hantavirus isolates collected from numerous geographic regions and hosts. we prepared rat immune sera to each virus and used these sera in hemagglutination inhibition (hai) tests, enzyme-linked immunosorbent assays (elisa), and plaque-reduction neutralization tests to establish the antigenic relationships among the isolates. our studies included viruses that had been partially characterized previously, as well as isolates for which little or no ... | 1994 | 8259655 |
| rna binding of recombinant nucleocapsid proteins of hantaviruses. | genes encoding the nucleocapsid (n) proteins of two hantaviruses, hantaan virus strain 76-118 (htn) and puumala virus strain cg 18-20 (puu), were expressed in escherichia coli as histidine-tagged proteins. they were purified by metal-chelate affinity chromatography under native or denaturing conditions to near homogeneity. the soluble form of htn n protein was associated with rna of e. coli. renatured n proteins were shown to bind in vitro transcribed rna representing the hantaviral small genomi ... | 1993 | 8480424 |
| hantaan virus infection with acute renal failure. | we report on 10 patients with acute renal involvement in hantaan virus infection observed at the department of nephrology, faculty of medicine, skopje, republic of macedonia, during a period of 3 years (october 1987-july 1990). eight patients were male and 2 were female, aged 37.5 +/- 4.8 years. the diagnosis of hantaan virus infection was proven by an indirect immunofluorescent and elisa test with a significant increase of the titer after a week to ranges from 1:512 to 1:2,048. percutaneous ren ... | 1995 | 8573000 |
| nucleocapsid- and virus-like particles assemble in cells infected with recombinant baculoviruses or vaccinia viruses expressing the m and the s segments of hantaan virus. | the formation of hantaan (htn) virus nucleocapsid-like structures (nls) or virus-like particles (vlp) from expressed gene products was investigated in two eukaryotic systems. baculovirus expression of the htn virus small segment (s), which encodes the viral nucleocapsid protein, resulted in assembly of nls inside infected insect cells. the nls and authentic ribonucleocapsids, prepared by detergent disruption of htn virions, had similar sedimentation characteristics and morphologies, and were rec ... | 1995 | 8578853 |
| antigenic characterization of hantaan and seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen. | hantaan virus (htn) and seoul virus (seo) are members of the genus hantavirus in the family bunyaviridae and are causative agents of hemorrhagic fever with renal syndrome. the complete and truncated nucleocapsid proteins (np) of htn and seo were expressed by a recombinant baculovirus system. antigenic characterization of the np using monoclonal antibodies (mabs) indicated that the binding sites for the serotype-specific mabs were located between amino acids (aa) 155 and 429. a western blot assay ... | 1998 | 9705385 |
| hantavirus seropositivity in israeli patients with renal failure. | the hantavirus is known to cause hemorraghic fever with renal syndrome (hfrs), which is widely spread in europe and asia. several reports have shown an association of hantavirus antibody titers and the occurrence of renal dysfunction. from these observations, it appears that the virus is widely distributed, and different strains prevail in various areas. in the present work we studied 81 patients with end-stage renal-failure under hemodialysis (hd) treatment, 55 with mild to moderate renal failu ... | 1998 | 9765032 |
| hantaan virus persistently infects marmoset b-lymphoblastoid cell line. | to investigate lymphoid cell hantavirus (hv) interaction, we examined the replication of the hv strains, hantaan 76-118 (htn), sr-11, and b-1 with the b95a, lym-1, cgm1, jm, jurkat, molt-4, at(l)5ky, bw5147, l1210, and vero/e6 cells. hv-growth was determined by immunofluorescence and focus-forming assays. lym-1 and vero/e6 supported the growth of all hv strains, and b95a only htn, while remaining cells did not sustain these strains. this is the first report to indicate the hv-replication in lymp ... | 1995 | 8593305 |
| [anti-hantavirus antibodies in school children in salvador, bahia]. | hantavirosis are emerging diseases in the americas. although considered rare in children, among the five cases diagnosed in brazil, one was in this age group. to know the serum-prevalence of hantavirosis in the pediatric group (schoolchildren) of low social economic level in salvador, bahia state, and to associate demographic, socio-economic and environmental factors to the serologic results, a standard questionnaire was applied and blood samples were collected from 379 students of two public sc ... | 1998 | 9789441 |
| bacterial expression of neutralizing mouse monoclonal antibody fab fragments to hantaan virus. | we amplified by polymerase chain reaction the heavy and light chain antibody genes of two mouse hybridomas secreting neutralizing monoclonal antibodies (mabs) to the g1 or g2 envelope proteins of hantaan virus, cloned them into the phagemid vector pcomb3, and expressed them in bacteria to yield fab fragments. expressed fab fragments had the same antigenic specificities for hantaan and seoul viruses as the complete parent mabs and were able to neutralize hantaan virus in plaque-reduction neutrali ... | 1996 | 8599211 |
| western blotting using recombinant hantaan virus nucleocapsid protein expressed in silkworm as a serological confirmation of hantavirus infection in human sera. | recombinant hantaan virus nucleocapsid protein expressed in silkworm larvae was applied as a serological diagnostic antigen in western blots (wb) of human sera. the sensitivity of this method was similar to that of the ifa test. hemorrhagic fever with renal syndrome (hfrs) and nephropathia epidemica diagnosed by their cross-reactivity in wb. the specificity of this method was higher than that of ifa test because the background was low. sera that exhibited high background staining in the ifa test ... | 1996 | 8645761 |
| [hemorrhagic fever renal syndrome-associated virus]. | | 1996 | 8650366 |
| stable expression of nucleocapsid proteins of puumala and hantaan virus in mammalian cells. | the development of an in vitro-system for the stable expression and the analysis of native hantavirus proteins using hantaviral cdna is of particular interest. as a first step the expression of the hantavirus nucleocapsid (n) proteins in mammalian cells was studied in more detail. the cdna of the s-rna segment of puumala virus strain cg-1820 and hantaan virus strain 76-118 was used for the construction of eucaryotic expression vectors that allow the generation and selection of mammalian cells ha ... | 1998 | 9857992 |
| isolation and characterization of dobrava hantavirus carried by the striped field mouse (apodemus agrarius) in estonia. | dobrava hantavirus (dob) was isolated from the striped field mouse (apodemus agrarius) trapped on saaremaa island, estonia, and its genetic and antigenic characteristics were subsequently analysed. phylogenetic analysis showed that the estonian dob strain, together with several wild strains carried by apodemus agrarius, forms a well-supported lineage within the dob clade. the topography of the trees calculated for the s, m and l nucleotide sequences of the estonian dob suggests a similar evoluti ... | 1999 | 10073697 |
| [dot-blot analysis in laboratory diagnosis of hemorrhagic fever with renal syndrome]. | a sensitive micro eia utilizing antigen or antibody dotted onto nitrocellulose filters (dot-elisa, dot-blot) has been developed for laboratory diagnosis of hemorrhagic fever with the renal syndrome (hfrs) by detecting the virus specific igm and igg in the sera of hfrs patients and the antigen in crude lung suspensions of wild rodents. measurements of specific igm and igg in paired hfrs sera collected during the first month of the disease showed clear-cut seroconversion and 100% correlation with ... | 1996 | 8669150 |
| ubiquity of hantaan virus infection with renal syndrome. | | 1996 | 8671879 |
| [the serological diagnosis of hemorrhagic fever with renal syndrome]. | early serological diagnosis of hemorrhagic fever with renal syndrome (hfrs) in 45 patients did not affect survival and policy of hemodialysis. however, the number of procedures, invasive in particular, to verify the diagnosis was much less. perfection of hfrs early diagnosis promotes optimization of the disease detection. | 1996 | 8677552 |
| human memory cytotoxic t-lymphocyte (ctl) responses to hantaan virus infection: identification of virus-specific and cross-reactive cd8(+) ctl epitopes on nucleocapsid protein. | hantaan virus, the prototypic member of the hantavirus genus, causes hemorrhagic fever with renal syndrome in humans. we examined the human memory t-lymphocyte responses of three donors who had previous laboratory-acquired infections with hantaan virus. we demonstrated virus-specific responses in bulk cultures of peripheral blood mononuclear cells (pbmc) from all donors. bulk t-cell responses were directed against either hantaan virus nucleocapsid (n) or g1 protein, and these responses varied be ... | 1999 | 10364276 |
| [detection of hemorrhagic fever virus-rna in renal tissue and peripheral blood in patients with hemorrhagic fever with renal syndrome]. | hemorrhagic fever virus (hfv) rna in renal tissue and blood samples from 21 patients with epidemic hemorrhagic fever with renal syndrome (hfrs) were examined by either in situ or blot hybridization using digoxigenin labelled hfv-cdna probe. hfv associated antigen and immune complex (ic) in renal tissue were also investigated simultaneously using immunofluorescence (if) technique. hfv-rna was detected in the renal specimen particularly renal tubular in 8/21 hfrs patients mostly of severe type ill ... | 1993 | 7906187 |
| nephropathia epidemica and puumala virus in austria. | to study the epidemiology of hantavirus infections in austria, 1215 humans and 596 rodents of different species were tested for the presence of antibodies against puumala and hantaan virus. direct virus identification by polymerase chain reaction in lung tissue of serologically positive rodents was performed to verify antibody results and to determine the genetic identity of viral rna by phylogenetic analysis of a part of the hantavirus m segment. for 32 of the 37 cases of nephropathia epidemica ... | 1999 | 10482022 |
| short report: simultaneous occurrence of dobrava, puumala, and tula hantaviruses in slovakia. | the prevalence of antibody to hantaviruses in slovakia (serum panel n = 2,133) was lower in the western part (0.54%) and higher in the eastern part (1.91%) of the country and was found to be significantly enhanced in a group of forest workers from eastern slovakia (5.88%). one-third of the igm-negative convalescent phase sera from patients with hemorrhagic fever with renal syndrome exhibited antibodies reacting predominantly with puumala virus antigen, while two-thirds had antibodies directed ma ... | 1999 | 10497981 |
| [a study on gastric mucosa damage, antigen location, and virus dissociation in patients with epidemic hemorrhagic fever]. | gastric mucosa damage was investigated with techniques of gastroscopy, gastric biopsy, antigen location with immunohistochemical enzyme staining and virus dissociation in 135 patients with epidemic hemorrhagic fever (ehf). we found that the major pathologic finding in gastric mucosa is hemorrhage of squamous shape. ehf virus antigen exists in epithelial cytoplasm of the superficial layer and lamina propria of gastric mucosa and the virus is dissociated from gastric mucosa. the virus is identifie ... | 1993 | 7907971 |
| [functional activity of hemagglutinins from hantaan viruses strains and use of hemagglutinating antigens for serodiagnosis hemorrhagic fever with kidney syndrome]. | hemagglutinins of hantaan virus strains isolated in southern far east of russia from patients with hemorrhagic fever with the renal syndrome and from rodents, natural vectors of the virus, were studied in the cross kinetic hemagglutination inhibition test with immune sera to the studied strains. the strains differed by the functional activity index. use of hemagglutinins of functionally active hantaan strains in serologic studies notably improves the diagnostic efficacy of the hemagglutination t ... | 1999 | 10500991 |
| n-acetylgalactosamine (galnac)-specific lectins mediate enhancement of hantaan virus infection. | summary. n-acetylgalactosamine (galnac)-specific lectins, dolichos biflorus agglutinin (dba), and soybean agglutinin (sba), enhanced hantaan (htn) virus infections in vero e6 and p388d1 cells. treatment of vero e6 cells with the lectins either before or during, but not after, virus inoculation resulted in lectin-mediated enhancement of infection (lme), indicating that galnac-specific lectin affects an early stage of the infection. lectin blot and facs analysis showed that the ability of htn viru ... | 1999 | 10542025 |
| [advances in research on pathogenesis of epidemic hemorrhagic fever]. | | 1993 | 7907974 |
| [study on the geographic distribution and serological typing of hfrs in hebei province]. | surveillance on the hfrs had been carried out during the period of 1986-1993. rodent density ranged from 4.6%-12.2% with r.norvegicus exceeding 60% in all kinds of rodents. hv antigen carrier rate of r.norvegicus was 1.3%-7.5%, much higher than that among other rodents. a significantly positive correlation had been observed between the hfrs morbidity and the r.norvegicus density. of 129 specimens from hfrs patients, 89% belonged to the type of seov. in the eastern and centre parts of hebei plain ... | 1996 | 8758402 |
| [circulation of seoul hantavirus in populations of synanthropic rodents and its significance in the incidence of hemorrhagic fever with renal syndrome in the primorye territory]. | the prevalence of seoul virus in the primorie territory is presented and its significance in the morbidity structure with regard to hemorrhagic fever with the renal syndrome (hlps) is discussed. epidemiological and clinical manifestations of hlps caused by seoul virus are described. the authors conclude that in the urban foci of hlps it is associated mainly with seoul virus, while in the rural foci, with hantaan virus. | 1999 | 10544448 |
| characterization of the hantaan nucleocapsid protein-ribonucleic acid interaction. | the nucleocapsid (n) protein functions in hantavirus replication through its interactions with the viral genomic and antigenomic rnas. to address the biological functions of the n protein, it was critical to first define this binding interaction. the dissociation constant, k(d), for the interaction of the hantaan virus (htnv) n protein and its genomic s segment (vrna) was measured under several solution conditions. overall, increasing the nacl and mg(2+) in these binding reactions had little imp ... | 1999 | 10559265 |
| [the diagnostic importance of determining antibodies in the urine to the virus of hemorrhagic fever with renal syndrome]. | blood sera and concentrated urine were tested for antibodies to hfrs virus. 18 patients were in acute period and 16 had suffered from hfrs 16 years before. indirect ifa test revealed antibodies in urine of 16 (88.9%) patients with hfrs. urine antibodies disappeared for 9-22 days. there were no antibodies in the urine of patients with the history of hfrs, though it was registered in high titre in the blood serum. thus, if the patient has antibodies to hfrs both in the blood and urine, it says abo ... | 1996 | 9045375 |
| serum and urine soluble hla class i antigen concentrations are increased in patients with hemorrhagic fever with renal syndrome. | in order to evaluate the association between the hantaan virus-induced cellular-immune response and clinical severity in patients with hemorrhagic fever with renal syndrome (hfrs). | 1997 | 9159038 |
| seroprevalence study of hantavirus infection in the community based population. | the genus hantavirus, family bunyaviridae, comprise at least 20 distinct viruses. ten of these have been linked to specific human diseases. hemorrhagic fever with renal syndrome has been recognized since the 1950s. hantavirus pulmonary syndrome was recognized in the united states in 1993. epizootiologic studies of norway rats in the baltimore area have shown that rodent infections with hantaviruses are common and geographically widespread with prevalence reaching 44% in tested rat populations. t ... | 1999 | 10628132 |
| persistent hantavirus infections: characteristics and mechanisms. | hantaviruses include serious human pathogens that are maintained in nature in persistently infected rodents and that can also persistently infect cultured mammalian cells, causing little or no cytopathology. the mechanisms of hantavirus persistence are only beginning to be explored. recent data point to subtle changes in the viral genome that might result in the differential regulation of replication and lead to persistence. | 2000 | 10664598 |
| detection of hantaan and seoul viruses by reverse transcriptase-polymerase chain reaction (rt-pcr) and restriction fragment length polymorphism (rflp) in renal syndrome patients with hemorrhagic fever. | hemorrhagic fever with renal syndrome (hfrs), also called korean hemorrhagic fever (khf), is the most common cause of acute renal failure in the far east. two serotypes of hantavirus, hantaan and seoul viruses are known pathogens for hfrs in korea. | 2000 | 10711409 |
| [acute renal failure in hantavirus infections]. | background: the acute renal failure remains a diagnostic challenge for the clinician. case reports: between 1991 and 1996, acute renal failure caused by hantavirus infection was diagnosed in 4 previously healthy male patients. main symptoms consisted of fever, headache, arthralgia, lumbar and abdominal pain as well as a decline in diuresis. the ultrasonography showed a slight splenomegaly in 2 patients. the clinical chemistry showed elevated serum creatinine from 2.2 mg/dl to 6.7 mg/dl and throm ... | 2000 | 10808304 |
| implications of the analogy between recombinant cytokine toxicities and manifestations of hantavirus infections. | the etiologic hantavirus of the 1993 emergence of an acute pulmonary failure syndrome in the area around northwestern new mexico was quickly recognized as related to the hantaan virus responsible for the outbreak of korean epidemic hemorrhagic fever (ehf) among un troops in 1951. discovery of the new disease which was named the hantavirus pulmonary syndrome (hps) and its causative agent the sine nombre virus (snv) inspired detailed comparisons between the two disorders. major damage to the epith ... | 1998 | 10850356 |
| [experience in using regression analysis for assessing the quality of the diagnosis of hemorrhagic fever with renal syndrome]. | a new approach has been developed to evaluate the adequacy of hfrs clinical diagnosis, by using regression analysis of large selections. the hfrs morbidity rate and the antibody prevalence rate in man were viewed as a function and an argument of the function, respectively. an empiric model for one of the active hfrs endemic area (saratov province), characterizing the correlation between the immunity and morbidity rates has been elaborated. it calculates the theoretical morbidity level that corre ... | 1997 | 9182194 |
| transmission electron microscopic study of the hemorrhagic spots in patients with epidemic hemorrhagic fever in the early stage. | epidemic hemorrhagic fever (ehf) virus particles were found in the squamous epithelial cells and the capillary endothelial cells of the petechial spots located on the mucous membrane of the soft palate in 3 patients with severe early-stage ehf with transmission electron microscopy. the virus particles were round or oval in shape, about 100 nm in diameter, with a two-layer lipid envelope from which spikes were protruding. the nucleocapsid of the virus appeared to be hollow microfilamentoid or den ... | 1997 | 9183829 |
| genetic diversity, distribution, and serological features of hantavirus infection in five countries in south america. | since 1995 when the first case of hantavirus pulmonary syndrome (hps) was reported in patagonia, there have been more than 400 cases of hps reported in five countries in south america. the first case of hps was associated with andes (and) virus. in this study, we report on the genetic diversity, geographical distribution, and serological features of hantavirus infection in six countries in south america based on 87 hps cases from argentina, bolivia, chile, paraguay, and uruguay. an early immunog ... | 2000 | 10921972 |
| [outbreak of hemorrhagic fever with kidney syndrome in egor'ev district of moscow region]. | epizootological, serological, and molecular virological analysis of an outbreak of hemorrhagic fever with renal syndrome (hfrs) in the egoryevsk district of moscow region (september 1995-january 1996) has been carried out. hantavirus (puumala) antigen and virus-specific antibodies were isolated from bank voles captured in the endemic focus. anti-puumala antibodies were detected in the sera of all hfrs patients and in 2% healthy residents of the endemic focus. analysis of nucleotide sequence (rna ... | 2000 | 10971964 |
| [high homology between antigen of hfrsv with anti-idiotype antibody against hfrsy]. | to detect the structural characterization of genes of the anti-idiotype antibody and the antigen. | 1996 | 9206197 |
| evaluation of two commercially available immunoassays for the detection of hantavirus antibodies in serum samples. | hantaviruses are members of the family bunyaviridae and the spectrum of clinical symptoms in humans may vary from sub-clinical to severe haemorrhagic fever with renal syndrome (hfrs) or pulmonary syndrome (hps). several serotypes have been described from which at least five are pathogenic to humans. each serotype has a different animal reservoir and geographical distribution. in the acute phase of the disease the clinical diagnosis may be confirmed by serology or by polymerase-chain reaction (pc ... | 2000 | 10996116 |
| [role of leptotrombidium (l.) scutellare in transmission of human diseases]. | a series of cases of hemorrhagic fever with renal syndrome (hfrs) in shaanxi province and autumn-type tsutsugamushi disease in jiangsu province were studied during 1988 to 1990 and 1986 to 1993 to explore the role of leptotrombidium (l.) scutellare in transmission of these diseases. results showed that l. (l.) scutellare is a dominant strain of mites inhabiting in rats and its seasonal fluctuation conformed to the seasonal distribution of these diseases. l. (l.) scutellare could naturally infect ... | 1996 | 9208519 |
| genetic diversity of hantaviruses isolated in china and characterization of novel hantaviruses isolated from niviventer confucianus and rattus rattus. | the antigenic and genetic properties of 46 hantaviruses from china, 13 from patients, 23 from rodents, and 10 from unknown hosts, were compared with those of other hantaviruses. the viruses were classified as either hantaan (htn) or seoul (seo) viruses. a phylogenetic analysis of the partial m (300 bp) and s (around 485 bp) genomes of htn viruses identified nine distinct genetic subtypes, one consisting of isolates from korea. the seo viruses were divided into five genetic subtypes, although the ... | 2000 | 11118357 |
| genetic diversity of apodemus agrarius-borne hantaan virus in korea. | hantaan (htn) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (hfrs), was first isolated in 1976 from lung tissues of striped field mice (apodemus agrarius) captured in songnae-ri, kyungki-do, korea. to clarify the genetic diversity and phylogenetic relationship among korean strains of htn virus, viral sequences of the partial s and m segments were amplified from lung tissues of 24 seropositive striped field mice captured between 1989 and 1998 at 11 sites in ... | 2000 | 11129640 |
| hantavirus nucleocapsid protein is expressed as a membrane-associated protein in the perinuclear region. | black creek canal virus (bccv) is a new world hantavirus which is associated with hantavirus pulmonary syndrome. we have examined the site of expression of the bccv nucleocapsid protein (nbccv) in the absence of bccv glycoproteins and found that the majority of the protein is localized to the golgi region. immunofluorescence analysis of bhk21 cells expressing the nbccv and la crosse virus nucleocapsid protein (nlacv) showed different intracellular localization patterns of these proteins within t ... | 2001 | 11160679 |
| ebola and hantaviruses. | | 1997 | 9348164 |
| [the results of the study of hemorrhagic fever with renal syndrome in the southern russian far east]. | | 1997 | 9432862 |
| evaluation of a pan-reactive hantavirus enzyme immunoassay and of a hantavirus immunoblot for the diagnosis of nephropathia epidemica. | nephropathia epidemica (ne) caused by the hantavirus serotype puumala (puuv) is endemic in large parts of europe. the prognosis of this disease is usually good. however, a rapid serological diagnosis is important to differentiate ne from potentially more severe renal conditions. | 2001 | 11255099 |
| [epidemiology of hantaviruses in baden-wurttemberg]. | hantavirus, originally named after the hantaan river in korea, is the aetiologic agent for the hemorrhagic fever with renal syndrome (hfrs) in the asian region, in the americas for the hantavirus pulmonary syndrome (hps). in middle europe hantaviruses are responsible for the "nephropathia epidemica" (ne), a mild form of hfrs. hantaviruses belong to the family of bunyaviridae. like other members of this family their genome consists of three segments of single stranded rna (ss-rna) leading to var ... | 2001 | 11285748 |
| genetic and serotypic characterization of sin nombre-like viruses in canadian peromyscus maniculatus mice. | in canada, hantavirus infected deer mice (peromyscus maniculatus) have been collected from british columbia to newfoundland. partial sequencing of g1 and n protein encoding regions from canadian peromyscus maniculatus-borne hantaviruses demonstrated the existence of significant genotypic divergence among strains. phylogenetic analysis showed that sin nombre (sn)-like viruses from eastern and western canadian deer mice can be divided into at least two broad-based genogroups. sequencing of mitocho ... | 2001 | 11311430 |
| characterization of neutralizing monoclonal antibody escape mutants of hantaan virus 76118. | neutralizing monoclonal antibody (mab) escape mutants of hantaan virus were generated using mabs to envelope protein g1 (16d2) and g2 (11e10). the mutant viruses (mu16d2 and mu11e10), lacked reactivity only to the selecting mab, or a mab belonging to the same antigenic site. both mutants had a single amino acid (a.a.) substitution. the a.a. substitution, found in mu16d2, was different from that found in another mutant selected with the same mab (16d2). although mab 11e10 immunoprecipitated g2 pr ... | 1998 | 9505967 |
| hantavirus infection induces the expression of rantes and ip-10 without causing increased permeability in human lung microvascular endothelial cells. | sin nombre virus (snv) and hantaan virus (htn) infect endothelial cells and are associated with different patterns of increased vascular permeability during human disease. it is thought that such patterns of increased vascular permeability are a consequence of endothelial activation and subsequent dysfunction mediated by differential immune responses to hantavirus infection. in this study, the ability of hantavirus to directly induce activation of human lung microvascular endothelial cells (hmve ... | 2001 | 11390609 |
| on the history of the study of haemorrhagic fever with renal syndrome in eastern russia. | | 2001 | 11390737 |
| clinical characterization of dobrava hantavirus infections in germany. | there is increasing evidence that dobrava (dobv) but not hantaan (htnv) hantavirus is a hemorrhagic fever with renal syndrome (hfrs) causing agent in central europe. however, only single clinical cases of hfrs due to acute dobv infection have been described so far. we report on three male patients from a non-endemic hantavirus focus in northern germany who suffered from mild to moderate hfrs strongly resembling nephropathia epidemica. serotyping by detection of hantavirus species-specific neutra ... | 2001 | 11393382 |
| truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections. | truncated recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), seoul virus (seov), and dobrava virus (dobv) were expressed by a baculovirus system. the truncated rnps, which lacked 49 (rnp50) or 154 (rnp155) n-terminal amino acids of the nps of htnv, seov, and dobv, were able to differentiate htnv-, seov-, and dobv-specific immune sera. recombinant np50s retained higher reactivities than rnp155s and were proven useful for enzyme-linked immunosorbent assay (elisa). the elisas based o ... | 2001 | 11427545 |
| nucleotide sequence of nucleocapsid protein (n) of hantaan virus isolated from a korean hemorrhagic fever patient. | the nucleotide sequence of the nucleocapsid protein (n) coding region of a hantaan virus strain (cfc94-2) isolated from a korean hemorrhagic fever (khf) patient was determined by sequencing a series of deletion mutants. comparison of the n coding sequence of cfc94-2 to the sequence of the prototype of hantaan virus, strain 76-118 reveals a 4.97% difference in the nucleotide sequence and a 1.6% difference in the deduced amino acid sequence. the rate of amino acid sequence variation in n protein o ... | 1997 | 9524814 |
| interaction between molecules of hantavirus nucleocapsid protein. | intermolecular interactions of tula hantavirus n (nucleocapsid) protein were detected in the yeast two-hybrid system, prompting further attempts to study this phenomenon. using chemical cross-linking and immunoblotting it was shown that the n protein from purified virus and from infected cell lysates as well as recombinant protein produced in a baculovirus expression system are capable of forming dimers, trimers and multimers, thus confirming the capacity of the protein molecules to interact wit ... | 2001 | 11457990 |
| [a study on the pathogenetic role of the viral structural proteins of hemorrhagic fever with renal syndrome]. | in order to clarify the pathogenesis of hemorrhagic fever with renal syndrome (hfrs), we detected hfrs virus structural proteins expressed in perepheral blood mononuclear cells (pbmc) by using immunohistochemical technique. meanwhile we also determined plasma endothelin (et), substance p (sp) and some sensitive markers of renal function with radioimmunoassay. the result showed that in hfrs patients, from the day of admission up to the thirteenth day of illness, the antigens of hfrs virus membran ... | 1996 | 9592317 |
| [an epidemic of hemorrhagic fever with renal syndrome in croatia in 1995]. | in the spring of 1995, the largest outbreak of hemorrhagic fever with renal syndrome (hfrs) so far was recorded in croatia. there were 125 patients reported to the national croatian institute of public health, department of epidemiology. the disease occurred simultaneously in several localities, some of them close to the previously known natural foci (mala kapela, western slavonia); the focus on dinara was newly discovered. war circumstances in croatia were closely related to this outbreak. ther ... | 1997 | 9658776 |
| hemorrhagic fever (puumala virus infection) with ocular involvement. | puumala virus infection (nephropathia epidemica) is a disease in the group of hemorrhagic fevers with renal syndrome causing ocular manifestations, e.g. transient myopia and changes in intraocular pressure. | 1998 | 9782434 |
| dna vaccination with the hantaan virus m gene protects hamsters against three of four hfrs hantaviruses and elicits a high-titer neutralizing antibody response in rhesus monkeys. | four hantaviruses-hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv) and puumala virus-are known to cause hemorrhagic fever with renal syndrome (hfrs) in europe and asia. htnv causes the most severe form of hfrs (5 to 15% case-fatality rate) and afflicts tens of thousands of people annually. previously, we demonstrated that dna vaccination with a plasmid expressing the seov m gene elicited neutralizing antibodies and protected hamsters against infection with seov and htnv. here, we r ... | 2001 | 11507192 |
| [the therapeutic effect of purified human leucocytic interferon-alpha on hemorrhagic fever with renal syndrome]. | in order to know the therapeutic effect of purified human leucocytic interferon-alpha on hemorrhagic fever with renal syndrome diagnosed early (< or = 5 days). we treated 23 cases with clinical prognosis and antiviral effect significantly better than those of 21 cases in a random control group. the membrane protein (mp), nucleo protein (np) in peripheral blood mononuclear cells (pbmc) and urine, the serum antibodies to mp and np were determined before treatment and 2, 4, 6, 8, 10 days after the ... | 1997 | 9812566 |
| [serotyping of hemorrhagic fever with renal syndrome in hubei province]. | ninety-two serum specimens, positive for antibodies against hemorrhagic fever with renal syndrome (hfrs) virus in initial screening with immunofluorescence assay technic (ifat), were serotyped with micro cell pathogenic effects neutralization test based on preliminary epidemiological classification of epidemic foci of hfrs throughout the province to find out serological evidence of hfrs typing in hubei province. it was found that 48 of the specimens were belonged to type i (htn) accounting for 5 ... | 1997 | 9812603 |
| the hantaan virus glycoprotein precursor is cleaved at the conserved pentapeptide waasa. | the medium segment of the tripartite negative-stranded rna genome of hantaviruses encodes for the predicted glycoprotein precursor gpc. we have demonstrated here the expression of the glycoprotein precursor of hantaan virus following transfection of mammalian cells. the cleavage of the precursor into the glycoproteins g1 and g2 followed the rules for signal peptides and seemed to occur directly at the pentapeptide motif "waasa." our data indicate that the signal peptidase complex is responsible ... | 2001 | 11689045 |
| [seroepidemiologic study on hemorrhagic fever with renal syndrome virus in the migration areas of the three-gorges reservoir chongqing, region, yangtze river]. | to ascertain both the immune level and latent infection status among the migrants from the three-gorge reservoir, chongqing region. | 2001 | 11718068 |
| [hemophagocytic syndrome and hemorrhagic fever with renal syndrome]. | | 1998 | 9819588 |
| [acute hantavirus infection caused by a genetically newly identified viral strain. severe and complicated course of hemorrhagic fever with renal syndrome (nephropathia epidemica)]. | a 49-year-old patient, a hobby hunter, fell ill acutely with joint and limb pain, abdominal pain, nausea and subfebrile temperatures. at hospitalization, the patient was in bad general condition, showing ascites and lid edema, and acute renal failure was diagnosed. | 1999 | 10081288 |