| partial characterization of a hantavirus isolated from a clethrionomys glareolus captured in belgium. | a hantavirus was isolated in vero-e6 cells from lungs of a free living bank vole (clethrionomys glareolus) captured in turnhout, province of antwerp--northern part of belgium. with help of monoclonal antibodies the belgian hantavirus isolate could be clearly differentiated from hantaan virus strain 76-118, prospect hill virus strain ph1 and sr11, a hantavirus isolated from laboratory wistar rat in japan, but not from the nephropathia epidemica virus strain hällnäs. | 1987 | 2886029 |
| hemorrhagic fever with renal syndrome in yugoslavia: detection of hantaviral antigen and antibody in wild rodents and serological diagnosis of human disease. | lung tissues from 547 rodents and 26 insectivores captured between 1981 and 1984 in central bosnia (fojnica) and central serbia (cacak), 2 regions known to be endemic for hemorrhagic fever with renal syndrome (hfrs), were examined for hantaviral antigen by the indirect immunofluorescent antibody technique. antigen was detected in 17/231 apodemus flavicollis, 3/187 a. sylvaticus, 1/46 a. agrarius, 4/32 mus musculus, and 3/28 clethrionomys glareolus. in addition, antibodies against hantaan and puu ... | 1988 | 2900550 |
| hemorrhagic fever with renal syndrome (nephropathia epidemica) in norway: seroepidemiology 1981-1985. | an indirect fluorescent antibody technique (ifat) was applied to serologically confirm the clinical diagnosis in 507 nephropathia epidemica (ne) suspected patients. hantaan virus (hv), the agent of korean hemorrhagic fever, which is serologically related to the ne agent, was used as antigen. both igg and igm reactions were detected. high levels of igg antibodies to hv were common, even in the acute phase of illness. over a 5-year period, a total of 35% of the ne suspected patients revealed antib ... | 1988 | 2900551 |
| seroepidemiology of haemorrhagic fever with renal syndrome in bulgaria. | during the period of 1954-1986, 399 cases of haemorrhagic fever with renal syndrome (hfrs) were registered in bulgaria with 63 (15.7%) deaths. three hundred serum samples from 214 patients who had contracted the disease from 1957 to 1986 were investigated by indirect fluorescent antibody test (if-at). as antigen vero-e6 cells infected with the asian hantaan virus were used, as well as lung sections from bank voles (clethrionomys glareolus) infected with strains udmurt and kazan 6-cg from the eur ... | 1988 | 2902774 |
| serological evidence of hantavirus infection in laboratory rats and personnel. | laboratory-acquired haemorrhagic fever with renal syndrome (hfrs) has been reported in many countries. a serological survey of laboratory white rats and of laboratory personnel for antibodies to hantaviruses was conducted in singapore. forty-four per cent (143/329) of rats were seropositive by the indirect immunofluorescent antibody test but none had hantaviral antigens in lung tissues. two of 74 laboratory personnel were seropositive but neither had a history of clinical illness. the high serop ... | 1988 | 2906333 |
| immune spleen cell-mediated protection against fatal hantaan virus infection in infant mice. | to clarify the basis of the age-dependent susceptibility of infant mice to fatal meningoencephalitis caused by hantaan virus, we investigated the ability of spleen cells from immune and nonimmune weanling balb/c mice to confer protection to syngeneic infant mice. intraperitoneal transfer of 10 x 10(7), 5 x 10(7), 2.5 x 10(7), and 1.2 x 10(7) immune spleen cells to infant mice 24 hr after intracerebral challenge with 100 50% lethal doses of hantaan virus (strain 76-118) resulted in 100%, 70%, 64% ... | 1985 | 2857753 |
| pathogenesis of hantaan virus infection in suckling mice: clinical, virologic, and serologic observations. | hemorrhagic fever with renal syndrome (hfrs) is a debilitating disease of humans caused by hantaan virus (hv), the prototype member of a newly proposed genus of bunyaviridae. studies of hv pathogenesis have been limited by the absence of a well defined model for a virus-induced disease state. in an attempt to devise a model for hv pathogenesis in laboratory rodents, newborn outbred suckling icr mice were shown to be uniformly susceptible to lethal infection with non-mouse adapted hv by intracere ... | 1985 | 2858987 |
| serological evidence of hantaan virus infection in the united states. | we found low titers of fluorescent antibodies against hantaan virus, the etiologic agent of korean hemorrhagic fever, in sera from 7 of 1,035 patients with febrile illnesses of unknown origin and from 6 of 664 blood donors in the united states. all but 1 of these individuals possessed neutralizing antibodies against hantaan virus. this was a 31-year-old research technician who had worked with laboratory rodents with virus-induced tumors, but had not traveled abroad, suggesting that infection wit ... | 1985 | 2858988 |
| [serological demonstration of the presence of hantaan virus in rattus norvegicus rats in an urban environment in france]. | | 1987 | 2951696 |
| [serological demonstration of the presence of hantaan virus in rattus norvegicus rats in a rural environment in france]. | | 1987 | 2951694 |
| morphology and morphogenesis of viruses of hemorrhagic fever with renal syndrome (hfrs). i. some peculiar aspects of the morphogenesis of various strains of hfrs virus. | the morphology and morphogenesis of viruses of hemorrhagic fever with renal syndrome (hfrs) were studied by thin-section electron microscopy. ten strains of hfrs virus isolated in china and one strain isolated in korea were compared. the virions varied in size as well as in the shape and character of their inner components. both intracellular and extracellular ribosome-like, electron-dense, virus-associated granules were seen. a viral antigen layer was often visualized on the surface of hfrs vir ... | 1985 | 2984144 |
| [sero-epidemiological study in mauritania (1985-1986): incidence of treponematosis, hepatitis b virus, hiv virus and viral hemorrhagic fevers]. | a serological serosurvey was made in different ethnic groups of mauritania in 1985. a very high prevalence of hepatitis b markers was found with more than 20% of hbs antigen carriers. treponema specific antibodies in low-age classes observed is a reflect of endemic syphilis. the seroprevalence of antibody against hiv and viral haemorrhagic fever viruses (rift valley fever, crimean-congo haemorrhagic fever and haemorrhagic fever with renal syndrome) was very low. | 1988 | 2900081 |
| conservation of antigenic properties and sequences encoding the envelope proteins of prototype hantaan virus and two virus isolates from korean haemorrhagic fever patients. | viruses isolated from the blood of two korean haemorrhagic fever patients were propagated in cell culture and compared to prototype hantaan virus which was isolated from apodemus mice. the antigenic properties of the human isolates were found to be closely related to hantaan virus by plaque reduction neutralization, haemagglutination inhibition and fluorescent antibody staining with both polyclonal and monoclonal antibodies. the medium genome segment of each human isolate was sequenced and compa ... | 1988 | 2900289 |
| growth experiment of hantaan virus in a549 cells: an attempt to improve the immunofluorescent antibody technique for hemorrhagic fever with renal syndrome. | an assay method for the infectivity of hantaan virus, a causative agent of hfrs (hemorrhagic fever with renal syndrome), was developed by the use of ifa (immunofluorescent antibody technique). with the aid of this method, the growth characteristics of hantaan virus, 76-118 strain, were followed in a549 cells. at a maximal moi (multiplicity of infection) of 1.6 vaiu (viral antigen-inducing units) per cell, the conventionally available value, plateau level potencies of the viral antigen and virus ... | 1986 | 2872077 |
| propagation of korean hemorrhagic fever virus in laboratory rats. | korean hemorrhagic fever virus (khfv) has been adapted to the wistar and fisher strains of rats. infection was detected by the appearance of specific antigen in the lung tissue of the infected rats at 14 to 64 days after inoculation and by the appearance of circulating antibodies in ther serum which reacted specifically with khfv antigen in the lungs of infected apodemus agrarius subsp. coreae 3 weeks after inoculation. distribution of antigen in rat tissues as determined by immunofluorescent st ... | 1981 | 6111538 |
| epidemiological investigations following an outbreak of hemorrhagic fever with renal syndrome in greece. | epidemiological investigations were conducted following an outbreak of hemorrhagic fever with renal syndrome (hfrs) which occurred in the state of epirus, northwestern greece, in july and august 1983. a total of 8 patients were hospitalized during the outbreak; 3 were severely ill and 1 died. a serosurvey made in may 1984 sampled 184 of the approximately 400 residents of the village of tsepelovo, where 4 patients resided, and found 12 (6.5%) persons, including convalescent sera from 4 patients, ... | 1986 | 2871769 |
| intraspecific transmission of hantaan virus, etiologic agent of korean hemorrhagic fever, in the rodent apodemus agrarius. | experimental parameters of infection and intraspecific transmission of hantaan virus, the etiologic agent of korean hemorrhagic fever, in apodemus agrarius rodents were determined. mice inoculated by the intramuscular route experienced viremia for about 1 week beginning on day 7. after 3 weeks, immunofluorescent and neutralizing antibodies were present and no mouse ever developed signs of acute illness. virus was recovered from lung, kidney, salivary gland, and liver, and virus excretion in urin ... | 1981 | 6116436 |
| [hantaan virus infections as a cause of acute kidney failure. 3 cases in west germany]. | three cases of hantaan virus infection (korean haemorrhagic fever) leading to acute renal failure are described. all three had mild haemorrhagic fever with a renal syndrome. it had started with acute fever followed by oliguria, proteinuria and microhaematuria (in two patients) in the further course of the disease, as well as urea and creatinine retention. one patient needed to be dialysed twice. hantaan virus-specific igg antibodies were demonstrated in all three patients; one also had igm antib ... | 1988 | 2897900 |
| electron microscope appearance of hantaan virus, the causative agent of korean haemorrhagic fever. | the morphology and morphogenesis of three strains of hantaan virus, which causes korean haemorrhagic fever (khf), were examined by thin-section and negative-contrast electron microscopy of infected a549 cell-culture specimens. in thin sections, virus was detected within cytoplasmic granular matrices (viroplasms) of the infected cells. virus particles were spherical (diameter 73 +/- 5 nm), and had an extremely electron-dense core (diameter 47 +/- 6.5 nm). replication and maturation of the virus s ... | 1981 | 6112446 |
| definition of three serotypes of hantaviruses by a double sandwich elisa with biotin-avidin amplification system. | a total of seven different isolates of hantaviruses from five mammalian species and six geographical areas were examined by a double sandwich elisa with biotin-avidin amplification system. three serotypes were recognized by comparing antigen titres to homologous and heterologous antibody and blocking antibody titres to homologous and heterologous antigens. serotype 1 included four strains: hantaan virus isolated from apodemus agrarius in south korea, hupei-i isolated from acute-phase serum from ... | 1985 | 3926952 |
| hantaan virus, aetiological agent of korean haemorrhagic fever, has bunyaviridae-like morphology. | spherical to oval particles with a unit membrane and subunit surface structure were demonstrated by negative-contrast staining of supernatant fluids of a-549 cell cultures infected with strain 76-118 of hantaan virus. the particles had an average diameter of about 95 nm, with a range of 80 to 110 nm. similar particles were isolated by buoyant density fractionation in sucrose gradients. in four separate experiments, infectivity cosedimented with 95 nm particles at buoyant densities from 1.15 to 1 ... | 1982 | 6121226 |
| intracerebral inoculation of suckling mice with hantaan virus. | | 1982 | 6125672 |
| korean hemorrhagic fever among rural residents in japan. | naturally occurring infection with korean hemorrhagic fever virus among four families living in rural areas of northern and central japan was proved by the demonstration of antibody to the virus. furthermore, korean hemorrhagic fever virus antigen was detected in the lung tissues of wild rodents captured in the endemic area. these findings indicate the endemic presence of korean hemorrhagic fever virus on honshu island, japan. | 1981 | 6118386 |
| morphological identification of the agent of korean haemorrhagic fever (hantaan virus)as a member of the bunyaviridae. | korean haemorrhagic fever (khf) (hantaan virus), a rodent-borne viral illness, is an important cause of human disease throughout much of asia and eastern europe. the agent responsible for khf has not yet been conclusively identified. plaque-purified khf virus was concentrated and then banded in a potassium tartrate gradient. material from the 1.17-1.19 g/ml band was examined by electron microscopy and particles with a morphology identical to that of the family bunyaviridae were found. the partic ... | 1982 | 6121225 |
| coding strategy of the s genome segment of hantaan virus. | hantaan virus is the type species of the recently recognized hantavirus genus of bunyaviridae. the small (s) rna segment of the negative-sense, tripartite genome was molecularly cloned and the nucleotide sequence was determined. the rna sequence derived from the cdna copy was found to contain 1696 nucleotides. a single open reading frame of sufficient size to encode the virus nucleocapsid protein was detected in the cdna corresponding to viral complementary-sense rna. rna transcripts of the cdna ... | 1986 | 3024404 |
| hantaan virus is related to hemorrhagic fever with renal syndrome in norway. | | 1982 | 6125745 |
| cell-mediated immunity to virus causing haemorrhagic fever with renal syndrome: generation of cytotoxic t lymphocytes. | cytotoxic t lymphocytes (ctls) were generated when spleen cells from mice infected with viruses causing haemorrhagic fever with renal syndrome were stimulated in vitro with syngeneic cells infected with viruses. these cytotoxic effector cells, with lyt2+ l3t4- markers on their surface, demonstrated h-2 restriction. ctls induced by hantaan virus (76-118 strain) or seoul virus (b-1 strain) showed cross-reactivity with infected target cells. hantaan virus infection induced a higher ctls response th ... | 1988 | 3137309 |
| hantaan virus replication: effects of monensin, tunicamycin and endoglycosidases on the structural glycoproteins. | the monovalent ionophore monensin, which interferes with cellular transport pathways, and the antibiotic tunicamycin, which prevents glycosylation of newly synthesized proteins, were used to examine hantaan virus particle formation and polypeptide synthesis. viral replication in the presence of either drug resulted in reduced antigen production as well as reduced yields of both intracellular and extracellular infectious virus. analysis of viral polypeptides synthesized in the presence of the dru ... | 1986 | 3083039 |
| geographical distribution and age related prevalence of antibody to hantaan-like virus in rat populations of baltimore, maryland, usa. | recent studies indicate that domestic rats (rattus norvegicus) from harbor-side areas within port cities of the united states are infected with a hantaan-like virus. the geographical distribution of seropositive rats may be extremely localized within these urban environments. we surveyed four widely separated residential sites distant from the harbor within baltimore, maryland, usa, to determine the geographical distribution and prevalence of antibody to hantaan-like virus in rats from urban are ... | 1985 | 3885776 |
| hantaan virus m rna: coding strategy, nucleotide sequence, and gene order. | the m genome segment of hantaan virus was molecularly cloned and the nucleotide sequence of cdna was determined. the virion rna is 3616 bases long with 3'- and 5'-terminal nucleotide sequences complementary for 18 bases. a single long open reading frame in the viral complementary-sense rna had the potential to encode 1135 amino acids or a polypeptide of 126,000 da. amino-terminal sequences of isolated g1 and g2 envelope glycoproteins were determined, revealing a gene order with respect to messag ... | 1987 | 3103329 |
| enzyme immuno assay for the detection of virus specific igg and igm antibody in patients with haemorrhagic fever with renal syndrome. | consecutive serum samples collected from 235 patients with haemorrhagic fever with renal syndrome (hfrs), between two days and two years after onset of disease, have been analysed for the presence of igg and igm type of antibodies specific for hanta-viruses. the sera were screened in parallel by a newly developed indirect immuno enzyme assay (eia) in parallel with indirect immunofluorescent antibody assay (ifa). in both tests the hantaan virus strain 76-118 was used as the antigen. the eia was m ... | 1988 | 2898929 |
| nucleotide sequence of the m segment of the genomic rna of hantaan virus 76-118. | | 1987 | 3114716 |
| muroid virus nephropathies and muroid viruses of the hantaan virus group. | hemorrhagic fever with renal syndrome (hfrs) came dramatically to the attention of western medicine in 1951 with the "new" disease becoming a major source of morbidity and mortality in american troops in korea. known there as epidemic hemorrhagic fever (ehf) or korean hemorrhagic fever (khf), it became apparent that it was the same disease as the endemic (epidemic) hemorrhagic nephroso-nephritis of the soviet far east and songo fever of the japanese army in manchuria. the conjecture that the mil ... | 1982 | 6134336 |
| immunofluorescence studies of disseminated hantaan virus infection of suckling mice. | hantaan virus, the etiological agent of korean hemorrhagic fever, was inoculated intracerebrally or intraperitoneally into suckling mice, and the course of the infection was followed by infectivity titration and immunofluorescence studies. mice became ill and were moribund by 13 to 14 days postinfection. in mice inoculated either intracerebrally or intraperitoneally, virus antigen was present in brain, heart, lungs, liver, and kidney. less consistently, specific fluorescence was observed in sple ... | 1983 | 6134678 |
| haemorrhagic fever with renal syndrome: memorandum from a who meeting. | haemorrhagic fever with renal syndrome (hfrs) is a public health problem throughout most of the european and asian land mass. although predominantly associated with rural areas, it is now being recognized as an urban problem in some countries, and also presents a particular hazard to laboratory staff who use rodents for biomedical research. in wild rodents (rats, mice and voles) the infection is asymptomatic. human infection with the hfrs agent(s) is sporadic, but under special circumstances epi ... | 1983 | 6134591 |
| disseminated intravascular coagulation in korean hemorrhagic fever. | to investigate the nature and role of coagulation and complement alterations in the pathogenesis of korean hemorrhagic fever (khf), the profiles from the early stages in 27 male patients were serially evaluated. evidence of disseminated intravascular coagulation (dic) was observed in 14 of the 27 patients (51.8%) sometime during the course of the disease. the earlier the coagulation tests were performed, the more frequently the evidence of dic was found. the mean serum c3 concentration was signi ... | 1983 | 6141786 |
| [prevalence of ectoparasites, endoparasites, bacteria and anti-hantaan virus antibodies in wild rats captured in harbor area of shimizu, japan]. | | 1984 | 6431033 |
| [acute interstitial nephropathy secondary to hantaan virus infection]. | | 1984 | 6149540 |
| an immune adherence assay for discrimination between etiologic agents of hemorrhagic fever with renal syndrome. | antigens of the viruses that cause hemorrhagic fever with renal syndrome (hfrs) and antibodies to these antigens were titrated in an immune adherence hemagglutination (iaha) test and a cf test. vero-e6 cells infected with the sr-11 strain of hfrs virus, an isolate from rats associated with a laboratory outbreak in japan, and the 76-118 strain of hantaan virus, the etiologic agent of korean hemorrhagic fever, were used as antigens in the iaha, cf, and indirect immunofluorescent antibody (ifa) tes ... | 1984 | 6141206 |
| hemagglutination-inhibiting antibody in hemorrhagic fever with renal syndrome. | hemagglutination activity was demonstrated in a hantaan virus antigen prepared from infected suckling mouse brain by sucrose-acetone extraction. hemagglutination of goose erythrocytes was ph dependent and was optimal at ph 6.0-6.2. immunofluorescent and hemagglutination-inhibiting antibodies were significantly correlated in 80 human and 13 animal sera. hemagglutination-inhibiting antibody was present in some patients 20 years after the onset of hemorrhagic fever with renal syndrome. the hemagglu ... | 1984 | 6150063 |
| positive serological evidence that hantaan virus, the etiologic agent of hemorrhagic fever with renal syndrome, is endemic in canada. | hemorrhagic fever with renal syndrome is endemic in most parts of the world. the etiological agent in known as hantaan virus. more than 2000 canadian sera from all age groups and provinces were tested for the presence of antibody to this virus by the indirect immunofluorescent antibody technique. the frequency of seropositivity ranged from 0.3 to 4.0%, with the national average being 1.4%. newfoundland and new brunswick in the atlantic region had the highest positivity, being higher than hyperen ... | 1984 | 6150755 |
| characterization of hantaan virions, the prototype virus of hemorrhagic fever with renal syndrome. | hantaan virus, strain 76-118, was propagated to high titer in a clone of vero cells, and infectious virions were successfully concentrated and purified. infectivity and virus antigenic activity were closely associated with a virus particle that exhibited a sedimentation rate indistinguishable from a representative member of the bunyaviridae. purified virions sedimented to a density of 1.16-1.17 in sucrose and 1.20-1.21 in cesium chloride. detergent disruption of virions resulted in a nucleocapsi ... | 1983 | 6140290 |
| [acute kidney failure caused by hantaan virus. case report from the west germany]. | a man aged 41 years who spent his leisure time in a marshy region infested with brown rats developed an infectious disease with high fever associated with diarrhoea, arthralgia and lumbago. in the course of the disease an acute renal failure occurred not requiring dialysis which spontaneously receded. histology revealed signs of an acute renal failure with sparse interstitial round-cell infiltrations. serological investigations showed a rising titre against hantaan virus, strain cg 1820 (1:1024) ... | 1986 | 3080305 |
| hantaan virus. | | 1984 | 6151600 |
| two cases of hemorrhagic fever with renal syndrome in northern greece. | two cases of hemorrhagic fever with renal syndrome were diagnosed in woodcutters presumably exposed to wild rodents and their urine in a forest in northern greece. the disease was characterized by acute renal insufficiency without hemorrhagic manifestations. one patient required hemodialysis, but both recovered without sequelae and developed a fourfold increase in titer of antibody to hantaan virus, as determined by an immunofluorescence test. these are the first reported cases of hemorrhagic fe ... | 1984 | 6145743 |
| [hemorrhagic fever with renal syndrome due to hantaan virus or a serologically related virus]. | seven cases of acute renal failure consecutive to haemorrhagic fever with renal syndrome (hfrs) due to the hantaan virus or to a serologically related virus are reported. these cases were observed in north-eastern france between march, 1983 and january, 1984. all patients were of rural origin and had been in contact with field mice. the predominant initial clinical symptoms were signs of infection and diffuse muscle pain, without evidence of haemorrhage. however, massive proteinuria was noted, a ... | 1984 | 6151176 |
| clinical and serological diagnosis of nephropathia epidemica, the mild type of haemorrhagic fever with renal syndrome. | the serological diagnosis of nephropathia epidemica (ne), the mild european type of haemorrhagic fever with renal syndrome (hfrs), was studied by means of an indirect immunofluorescent antibody technique (ifat) in a large outbreak in finland (morbidity 1.4/1000 population). acetone-fixed sections of lung of bank voles (clethrionomys glareolus) naturally infected with puumala virus served as antigen. altogether 65 patients were followed serologically for up to 20 months. serological results obtai ... | 1984 | 6151960 |
| synergistic antiviral effects of ribavirin and the c-nucleoside analogs tiazofurin and selenazofurin against togaviruses, bunyaviruses, and arenaviruses. | binary combinations of the n-nucleoside ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) and the c-nucleoside analog selenazofurin (2-beta-d-ribofuranosylselenazole-4-carboxamide) or tiazofurin (2-beta-d-ribofuranosylthiazole-4-carboxamide) were tested in vitro for activity against venezuelan equine encephalomyelitis, japanese encephalitis, yellow fever, rift valley fever, korean hemorrhagic fever, and pichinde viruses. the 50% effective dose for each compound alone or in a series ... | 1984 | 6151377 |
| viruses of hemorrhagic fever with renal syndrome (hfrs) grouped by immunoprecipitation and hemagglutination inhibition. | nine viruses antigenically related to hantaan virus that were isolated from various rodents in several countries and from a patient were examined by immunoprecipitation and by hemagglutination inhibition (hi). the immunoprecipitation tests were done with a monoclonal antibody that reacted with glycoprotein g2 and an antibody against a nucleocapsid protein. the viruses were classified into four subtypes on the basis of the molecular weights of their precipitated polypeptides and the pattern of re ... | 1987 | 3117723 |
| expression of the hantaan virus m genome segment by using a vaccinia virus recombinant. | a cdna containing the complete open reading frame of the hantaan virus (htn) m genome segment has been cloned into vaccinia virus. this recombinant virus expresses two glycoproteins which are similar to the htn structural glycoproteins, g1 and g2, in molecular weight, cleavage pattern, and cellular distribution. both htn and recombinant vaccinia virus glycoproteins are exclusively associated with the golgi apparatus of the cell. despite this intracellular restriction, mice inoculated with the re ... | 1988 | 3123711 |
| development of inactivated vaccine against virus causing haemorrhagic fever with renal syndrome. | b-1 virus belonging to the hantavirus group was serially passaged in the brains of newborn mice. inactivated vaccine was prepared from the brains after inactivation with formalin and then purification by ultracentrifugation. the antigenic potency of this vaccine in vitro was determined by antibody-bound enzyme-linked immunosorbent assay (elisa) and serial diluted vaccine bound to an aluminium hydroxide gel was inoculated into balb/c mice to test immunogenicity. after two injections of this vacci ... | 1988 | 3138829 |
| laboratory-acquired infections with hantaan virus, the etiologic agent of korean hemorrhagic fever. | clinically apparent infections with hantaan virus, the etiologic agent of korean hemorrhagic fever, occurred in nine persons exposed to the virus at the korea university virus institute (seoul) between 1971 and 1979. all were directly related to trapping of wild rodents or work with naturally or experimentally infected wild and laboratory rodents in a designated animal suite. all infections were acquired during the months of november-april, and none was associated with accidental parenteral inoc ... | 1982 | 6127367 |
| seroepidemiology of hantaan virus. | | 1982 | 6129497 |
| [propagation and replication of hantaan virus in human endothelial cells in vitro]. | | 1988 | 3147808 |
| immunoperoxidase assay for the detection of specific igg antibodies to hantaan virus. | a technique, using indirect immunoperoxidase antibody (ipa), was developed for the detection of igg antibody to hantaan virus. the same protein a-peroxidase conjugate was used with mouse, rat and human sera. the ipa technique employs glass slides with air-dried gamma-ray-inactivated and acetone-fixed hantaan-infected vero-e6 cells. antibody titers detected by ipa was comparable to those detected by the indirect fluorescent antibody technique. | 1985 | 3882732 |
| analysis of hantaan virus rna: evidence for a new genus of bunyaviridae. | hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family bunyaviridae. nucleocapsid rna from hantaan virions and a control bunyavirus were examined for ribonuclease a (rnase a) sensitivity. both viruses exhibited a similar accessibility of rna within nucleocapsids to digestion by rnase a. complete digestion of the rna of both viruses was affected with high c ... | 1983 | 6419460 |
| isolation of hantaan virus, the etiologic agent of korean hemorrhagic fever, from wild urban rats. | urban rats captured in seoul and four nearby korean cities were found to have immunofluorescent antibodies reactive with hantaan virus, the etiologic agent of korean hemorrhagic fever (khf). serum antibodies were detected in 13% from 477 rattus norvegicus and 11% of 47 rattus rattus. hantaan viral antigen was found in pulmonary tissues of 42 animals, and hantaan virus was recovered from 23 rats, all but two of which were r. norvegicus. wistar rats were qualitatively much more sensitive than apod ... | 1982 | 6127366 |
| evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus. | titers of hemorrhagic fever with renal syndrome virus were estimated by counting foci stained with peroxidase-antiperoxidase or by using immunofluorescence methods. foci stained with pap were clearer and easier to count. the peroxidase-antiperoxidase method showed a linear relationship between virus concentration and the number of foci. a focus-reduction neutralizing antibody test was performed with immune rat sera and sera from patients. again, the peroxidase-antiperoxidase method proved to be ... | 1984 | 6440908 |
| an epidemiological survey of nephropathia epidemica in sweden. | the indirect immunofluorescence test (ift) using the hantaan virus (agent of korean hemorrhagic fever, khf) was applied for detection of antibodies to nephropathia epidemica (ne). from 103 patients with symptoms suggesting ne, 61% revealed khf/ne antibodies in the convalescent sera. all seropositive patients seemed to have acquired their disease in ne endemic areas (north of the 60th parallel). only patients found to have a typical clinical ne (fever, abdominal pain and renal engagement) were se ... | 1983 | 6417769 |
| monoclonal antibodies specific for hantaan virus. | six hybridoma cell line producing monoclonal antibodies to hantaan virus were established by fusion of ns-1 mouse myeloma cells with spleen cells of mice immunized with hantaan virus strain 76-118. the specificity of these monoclonal antibodies was established by immunoblotting analysis and immunofluorescence. five of the clones reacted with antigens on the cell surface and in the cytoplasm, and one clone reacted with a determinant expressed only in the cytoplasm of the infected cells. two of th ... | 1983 | 6408643 |
| infectivity titration of hemorrhagic fever with renal syndrome virus: use of immune adherence hemagglutination for detection of virus growth. | serial dilutions of hemorrhagic fever with renal syndrome viruses were inoculated into vero-e6 cells in microplates. after 2 weeks of incubation, infected cells were disrupted by freezing and thawing, and virus antigens were detected by immune adherence hemagglutination. the infectivity titers of the virus as determined by this method were in close agreement with those obtained by the immunofluorescent antigen endpoint method. then, a neutralization method was established. japanese hemorrhagic f ... | 1984 | 6436305 |
| epitope mapping studies with neutralizing and non-neutralizing monoclonal antibodies to the g1 and g2 envelope glycoproteins of hantaan virus. | epitopes recognized by three g1-specific and two g2-specific neutralizing monoclonal antibodies to hantaan virus were mapped by sequence analyses of the complete m genome segments of neutralization escape variant viruses. for each variant, we detected nucleotide sequence substitutions which resulted in a single amino acid change in either the g1 or g2 protein. serological properties of the variant viruses correlated with changes identified by nucleotide sequence analyses. to map epitopes recogni ... | 1993 | 7504368 |
| [preparation and antigenic characterization of rat monoclonal antibodies against hemorrhagic fever with renal syndrome virus]. | 11 hybridoma cell lines secreting monoclonal antibodies (mcabs) against hemorrhagic fever with renal syndrome virus (hfrsv) were obtained in our laboratory by immunizing lou/c rats with hfrsv chen strain. these rat mcabs were characterized by radio-immunoprecipitation and western blotting methods, 2 directed against glycoprotein g2, 9 against nucleocapsid protein (np) of hfrsv. the results of neutralization (nt) and hemagglutination inhibition(hi) test showed that most of the mcabs specific for ... | 1994 | 7522942 |
| british soldier returns from bosnia with hfrs. | | 1995 | 7581633 |
| hantaan virus: identification of virion proteins. | sds-page and immunoprecipitation analyses were carried out on the virion and cell-associated proteins of hantaan virus, the causative agent of haemorrhagic fever with renal syndrome (hfrs). purified virions have a density of 1.17 g/ml in sucrose, and contain four proteins with molecular weights of 45 000 (45k), 56k, 72k and 200k, confirming recent evidence that the virus is a member of the family bunyaviridae. detergent treatment of virions indicates that the 45k protein is the virus nucleoprote ... | 1984 | 6540294 |
| broad-spectrum antiviral activity of 2-beta-d-ribofuranosylselenazole-4-carboxamide, a new antiviral agent. | the relative in vitro antiviral activities of three related nucleoside carboxamides, ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), tiazofurin (2-beta-d-ribofuranosylthiazole-4-carboxamide), and selenazole (2-beta-d-ribofuranosylselenazole-4-carboxamide), were studied against selected dna and rna viruses. although the activity of selenazole against different viruses varied, it was significantly more potent than ribavirin and tiazofurin against all tested representatives of the ... | 1983 | 6615611 |
| mixed natural focus of tick-borne encephalitis, tularemia and haemorrhagic fever with renal syndrome in west slovakia. | total of 923 small mammals of 7 species were collected in locality záhorská ves, west slovakia, in 1990-1992. among examined small mammal species it was clethrionomys glareolus (48.7% of total, 17.5% positive for tick-borne encephalitis (tbe) virus antibodies), apodemus flavicollis (29.7% of total, 17.5% positive), a. sylvaticus (11.3% of total, 16.3% positive), and microtus arvalis (6.2% of total, 10.5% positive). the most abundant tick species (larval and nymphal stages) on small mammals was i ... | 1995 | 7676942 |
| localization of hantaan viral envelope glycoproteins by monoclonal antibodies in renal tissues from patients with korean hemorrhagic fever h. | the role of viruses in several renal diseases is not documented clearly. the authors attempted to localize envelope glycoproteins of hantaan virus in biopsy specimens from patients with korean hemorrhagic fever (khf) as evidence of direct viral invasion of renal tissues. the authors studied sequential sections of kidney biopsy specimens from 23 of 35 patients with serologically confirmed khf diagnosed between june 1985 and december 1989. the sections were stained with the avidin-biotin-peroxidas ... | 1993 | 7692720 |
| a serotypic study of hemorrhagic fever with renal syndrome in rural china. | apodemus agrarius was trapped in the fields and rattus norvegicus was trapped within the houses in the villages of jiande county, a region in the zhejiang province of china endemic for hemorrhagic fever with renal syndrome (hfrs). antibodies to hantaviruses were detected in three (16.7%) of 18 a. agrarius and 12 (13.5%) of 89 r. norvegicus, whereas hantavirus antigens were detected in the lung tissues of four (22.2%) of 18 and nine (10.1%) of 89 of these rodents, respectively. three hantaviruses ... | 1995 | 7694967 |
| [electron microscopic study on the petechial hemorrhagic spots in patients with epidemic hemorrhage fever (ehf)]. | ehf viral particles were found in the squamous epithelial cells and capillary endothelial cells of the petechial spots located at the mucous membrane of the soft palate in cases of early stage of severe type ehf by transmission electron microscopy. the viral particles are round or oval in shape, about 100 nm in diameter with a lipid bilayer envelope from which spikes are protruding. the virions matured by budding through the intracytoplasmic membranes into the smooth surfaced vesicles. the morph ... | 1994 | 7720117 |
| serological evidence of hantaan virus in slovakia during 1989-1991. | nephropathia epidemica was supposed to be an european form of haemorrhagic fever with renal syndrome (hfrs). however, in 1989-1991 we found serological evidence of infection with hantaan virus in patients' sera from slovakia. higher titers of antibodies against hantaan virus were detected in sera of 6 out of 75 patients with febrile disease, renal failure and clinical diagnosis of hfrs. the higher titers of antibodies against puumala virus were detected in sera of 6 out of 75 patients. serologic ... | 1994 | 7726005 |
| experimental infection in newborn mice and rats by hemorrhagic fever with renal syndrome (hfrs) virus. | newborn mice and rats were inoculated intracerebrally (ic) or intraperitoneally (ip) with hantaan virus (76-118 strain) or hfrs-related virus (b-1 strain). the mortality and the influence on the increase of body weight in newborn mice were higher in the groups infected with the 76-118 strain than in the groups infected with the b-1 strain, while the b-1 strain was more virulent in rats than the 76-118 strain. virus isolation from rats inoculated with either strain was attempted 7 and 11 weeks af ... | 1984 | 6442757 |
| in-vivo activity of antivirals against exotic rna viral infections. | infection of humans by viruses belonging to the families of toga-, bunya-, and arenaviridae constitutes a major health problem worldwide and certain of the viruses have the potential to cause widespread epidemics. in the search for effective chemotherapy against these viruses several hundred nucleoside and nucleotide analogues have been screened for antiviral activity. of the compounds tested, ribavirin, has been shown in laboratory animal models to have significant inhibitory effects against ri ... | 1984 | 6208183 |
| enhancement of infectivity of hantavirus in cell culture by centrifugation. | centrifugation was introduced during virus adsorption to vero e6 cells to improve the infectivity of hantavirus. centrifugal adsorption of a stock solution of hantaan virus strain 76-118 to a monolayer of vero e6 cells enhanced virus infectivity depending on the centrifugation time and the centrifugal force. the maximum level of infectivity (3.1 x 10(6) ffu/ml) was enhanced after a 2 h centrifugation at 671 x g, which was almost 9-times higher than that of conventional adsorption of the virus at ... | 1994 | 7822463 |
| the 5' ends of hantaan virus (bunyaviridae) rnas suggest a prime-and-realign mechanism for the initiation of rna synthesis. | we examined the 5' ends of hantaan virus (htn) genomes and mrnas to gain insight into the manner in which these chains were initiated. like those of all members of the family bunyaviridae described so far, the htn mrnas contained 5' terminal extensions that were heterogeneous in both length and sequence, presumably because htn also "cap snatches" host mrnas to initiate the viral mrnas. unexpectedly, however, almost all of the mrnas contained a g residue at position -1, and a large fraction also ... | 1995 | 7637020 |
| seroprevalence study of hantavirus antibodies in pima indians with renal disease. | | 1995 | 7876639 |
| antigenic differences between two viruses, isolated in japan and korea, that cause hemorrhagic fever with renal syndrome. | hantaan virus (hv) 76-118, isolated from apodemus agrarius coreae in korea, and hemorrhagic fever with renal syndrome (hfrs) virus b-1, isolated from a rat in japan, were examined for polypeptide compositions and for differences in immune responses in rats. in immunoprecipitation experiments, a major polypeptide of ca. 50 kilodaltons (k) was detected with antisera against hv 76-118 in cell extracts from vero e6 cells infected with hfrs virus b-1, whereas three major polypeptides of 74 k (glycosy ... | 1984 | 6207309 |
| cross-neutralization of hantaviruses with immune sera from experimentally infected animals and from hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome patients. | plaque-reduction neutralization tests were done with eight of nine known representative hantaviruses and immune sera from experimentally infected animals and from patients with hemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps). results obtained with animal sera demonstrated each virus to be antigenically unique. neutralization with the hps patient sera was highest with sin nombre (sn) virus and to a lesser extent with black creek canal (bcc) virus. sera from kor ... | 1995 | 7594720 |
| [viral infections--clinical pictures and laboratory diagnosis. part 3: hantaan virus and hantaan-related viruses, hepatitis a and b]. | | 1993 | 7905848 |
| [detection of hemorrhagic fever virus-rna in renal tissue and peripheral blood in patients with hemorrhagic fever with renal syndrome]. | hemorrhagic fever virus (hfv) rna in renal tissue and blood samples from 21 patients with epidemic hemorrhagic fever with renal syndrome (hfrs) were examined by either in situ or blot hybridization using digoxigenin labelled hfv-cdna probe. hfv associated antigen and immune complex (ic) in renal tissue were also investigated simultaneously using immunofluorescence (if) technique. hfv-rna was detected in the renal specimen particularly renal tubular in 8/21 hfrs patients mostly of severe type ill ... | 1993 | 7906187 |
| comparison of virulence between seoul virus strain sr-11 and hantaan virus strain 76-118 of hantaviruses in newborn mice. | virulence of hantavirus strain of sr-11 seoul virus and hantaan 76-118 (htn) of hantaan virus were compared. infections of both strains were lethal in newborn mice. however, inoculum required to cause lethal infection was about 4,000 times higher for strain htn (1.65 x 10(3) pfu/mouse/ld50) than for strain sr-11 (0.36 pfu). thus, both strains were considered pathogenic to newborn mice but they possessed different levels of virulence. the assay system used for these strains in newborn mice proved ... | 1993 | 7901740 |
| [the study on elisa methods for detection of specific antibodies against hemorrhagic fever with renal syndrome virus in rat sera]. | three elisa methods were developed to detect specific antibodies against hemorrhagic fever with renal syndrome virus (hfrsv) in rats sera. it was shown that all three kinds of mcab-elisa were specific, sensitive and simple for the detection of hfrsv antibodies in rat sera. | 1994 | 7834708 |
| epidemiologic surveillance on the hemorrhagic fever with renal syndrome in china. | this article summarizes the contents and results of extensive surveillance on hemorrhagic fever with renal syndrome (hfrs) in china. data from surveillance on human hfrs, including morbidity and case fatality rate, trend of epidemics, periodicity of epidemics, factors related to epidemics, types of epidemic areas, environment structure of natural nidi, geographic distribution, seasonal distribution, age distribution, sex distribution and occupation distribution, as well as surveillance on animal ... | 1993 | 7908258 |
| strand-specific detection of hantaan virus rna sequences by in vitro dna amplification. | hantaan virus often causes a fatal human disease, hemorrhagic fever with renal syndrome (hfrs). an assay for strand-specific detection and quantitation of hantaan virus rna was developed based on the polymerase chain reaction (pcr). a protocol for the efficient detection of both genomic rna and its transcript is presented, in which a reverse transcriptase reaction (rtr) followed by pcr with two common primers was used to distinguish between positive- and negative-stranded rna. using this method, ... | 1994 | 7898391 |
| [advances in research on pathogenesis of epidemic hemorrhagic fever]. | | 1993 | 7907974 |
| a vaccinia virus-vectored hantaan virus vaccine protects hamsters from challenge with hantaan and seoul viruses but not puumala virus. | to investigate the ability of a vaccinia virus-vectored vaccine expressing the m and the s segments of hantaan (htn) virus (c. s. schmaljohn, s. e. hasty, and j. m. dalrymple, vaccine 10:10-13, 1992) to elicit a protective immune response against other hantaviruses, we vaccinated hamsters with the recombinant vaccine and challenged them with htn, seoul (seo), or puumala (puu) virus. neutralizing antibodies to htn virus were found in all vaccinated hamsters both before and after challenge. neutra ... | 1995 | 7666542 |
| [a study on gastric mucosa damage, antigen location, and virus dissociation in patients with epidemic hemorrhagic fever]. | gastric mucosa damage was investigated with techniques of gastroscopy, gastric biopsy, antigen location with immunohistochemical enzyme staining and virus dissociation in 135 patients with epidemic hemorrhagic fever (ehf). we found that the major pathologic finding in gastric mucosa is hemorrhage of squamous shape. ehf virus antigen exists in epithelial cytoplasm of the superficial layer and lamina propria of gastric mucosa and the virus is dissociated from gastric mucosa. the virus is identifie ... | 1993 | 7907971 |
| antigenic and molecular characterization of hantavirus isolates from china. | hemorrhagic fever with renal syndrome (hfrs) is caused by certain viruses in the genus hantavirus, family bunyaviridae, and is a major public health problem in china. by using molecular and serological tests, we characterized 15 hantaviruses isolated either from patients with hfrs or from rodents captured in endemic areas of china. by cross plaque-reduction neutralization tests performed with rabbit immune sera, we identified two serologically distinct groups of viruses, comprised of those relat ... | 1994 | 7909976 |
| characterization of the mode of hantaan virus infection in adult mice using a nested reverse transcriptase polymerase chain reaction: transient virus replication in adult mice. | a polymerase chain reaction (pcr) for the detection of hantavirus genome was established and applied to analyze the mode of infection of hantaan virus in adult icr mice. the cdna for the s genome segment of hantaan virus was reverse-transcribed from the total rna of organs of the infected mice. the sequence in the s genome segment of hantaan virus was successfully amplified by reverse transcriptase (rt)-pcr followed by nested pcr. in 5-week-old icr mice inoculated intraperitoneally with hantaan ... | 1995 | 7783676 |
| development of monoclonal antibodies against hantaan virus nucleocapsid protein. | forty-five hybridoma cell lines producing monoclonal antibodies against hantaan virus, the etiologic agent of hemorrhagic fever with renal syndrome, were generated by fusion of p3-x63-ag8.v653 myeloma cells with spleen cells of mice immunized with inactivated hantaan virus vaccine. among these, 38 antibodies were identified as binding to the 48-kda nucleocapsid protein by immunoblot assay or radioimmunoprecipitation. twenty-six of them were of the immunoglobulin g1 (igg1), nine were of the igg2a ... | 1995 | 7583920 |
| investigation on inactivated epidemic hemorrhagic fever tissue culture vaccine in humans. | from 1988 to 1992, 2479 volunteers were immunized by inactivated meriones unguiculatus kidney cell (mukc) vaccine. their seroconversion rates of neutralizing antibody were about 90% after 3 doses of the vaccine. no side effects were found. the vaccine stored for 14 months at 4 degrees c still had good immunogenicity. it was shown that the vaccine was safe, effective and stable. detailed observation was made in 75 volunteers. the results showed that the seroconversion rate by plaque reduction neu ... | 1994 | 7916278 |
| belgrade and hantaan hantaviruses--the causative agents of severe haemorrhagic fever with renal syndrome in children in serbia. | during an outbreak of haemorrhagic fever with renal syndrome (hfrs) in 1989, five children (3 girls, 2 boys, aged 6.8-16 years) with a severe form of the disease were treated; four of these were followed for 22-28 months. the main clinical features in all five patients were: fever, headache, myalgia, abdominal and back pain and vomiting; haemorrhagic syndrome was present in four children. renal syndrome presented with severe acute renal failure in all five patients. all patients recovered. serol ... | 1994 | 7917855 |
| domestic cases of hemorrhagic fever with renal syndrome in the united states. | although serologic studies have identified hantaviral infection in the united states, acute disease has not been recognized. this study describes 3 cases of domestically acquired hemorrhagic fever with renal syndrome (hfrs) in the united states. infection was due to a local strain of seoul virus (baltimore rat virus). a review of the clinical features indicated a mild illness characterized by nausea, vomiting, renal and liver failure similar to hfrs described elsewhere for rat-borne viruses. fol ... | 1994 | 7991040 |
| western blotting using recombinant hantaan virus nucleocapsid protein expressed in silkworm as a serological confirmation of hantavirus infection in human sera. | recombinant hantaan virus nucleocapsid protein expressed in silkworm larvae was applied as a serological diagnostic antigen in western blots (wb) of human sera. the sensitivity of this method was similar to that of the ifa test. hemorrhagic fever with renal syndrome (hfrs) and nephropathia epidemica diagnosed by their cross-reactivity in wb. the specificity of this method was higher than that of ifa test because the background was low. sera that exhibited high background staining in the ifa test ... | 1996 | 8645761 |
| an etiologic study of hemoglobinuria and blackwater fever in the kivu mountains, zaire. | between january 1985 and march 1986, in the high altitude area of kivu, eastern zaïre, 38 patients presenting with hemoglobinuria as main manifestation were investigated. profound glucose-6-phosphate dehydrogenase deficiency was detected in 4 patients, leptospirosis in 2 and hantaan virus infection in 2. hemolysis was doubtful (haptoglobin > 40 mg/dl, hemoglobin > 12 g/dl) in 2 patients. other potential causes of hemoglobinuria such as hemoglobinopathy, toxic agents, infectious diseases or blood ... | 1995 | 7794063 |
| belgrade virus, a cause of hemorrhagic fever with renal syndrome in the balkans, is closely related to dobrava virus of field mice. | belgrade virus is a recently described hantavirus that causes severe hemorrhagic fever with renal syndrome (hfrs) in people living in various parts of the balkan peninsula. nucleotide sequencing of the g2-encoding region in the medium (m) segment of the viral genome, reverse transcribed and amplified by the polymerase chain reaction, revealed the belgrade virus to be substantially different from hantaan virus and other major serotypes of hantavirus but identical to dobrava virus, a virus isolate ... | 1993 | 8102632 |
| association of serine in position 1124 of hantaan virus glycoprotein with virulence in mice. | hantaan virus (hv) of the genus hantavirus causes a fatal disease in suckling mice following intraperitoneal or intracerebral infection. hv cl-1, which was obtained from the 76-118 strain of hv by growth in vero e6 cells, exhibited high mortality rates in mice whereas mice infected with hv cl-2 survived without any clinical signs. to determine the molecular basis for the marked difference in virulence, we compared the nucleotide sequences of the large (l), medium (m) and small (s) segments of hv ... | 1994 | 7964639 |
| expression strategy of the m genome segment of hantaan virus. | the medium (m) genome segment of hantaan virus encodes the envelope glycoproteins, g1 and g2, in a continuous open reading frame with a gene order of 5'-g1-g2-3' with respect to the virus-complementary sense rna. because potential translation initiation codons and amino acids constituting typical signal sequences precede both the g1 and g2 genes, we sought to determine if g1 and g2 can be expressed independently. to investigate translational requirements for g1 and g2, we constructed m segment g ... | 1994 | 8165863 |
| high frequency of antibodies to hantaan virus and hepatitis c virus in chronic haemodialysis patients. coincidence or cross-reaction? | to address the question of whether there is any coincidence or cross-reaction between hepatitis c virus (hcv) and hantaan virus (both rna arboviruses), as well as to assess the frequency of antibodies to the above viruses amongst chronic haemodialysis patients in our region. | 1993 | 8258753 |
| serological relationships among viruses in the hantavirus genus, family bunyaviridae. | we examined the serological relationships among 32 hantavirus isolates collected from numerous geographic regions and hosts. we prepared rat immune sera to each virus and used these sera in hemagglutination inhibition (hai) tests, enzyme-linked immunosorbent assays (elisa), and plaque-reduction neutralization tests to establish the antigenic relationships among the isolates. our studies included viruses that had been partially characterized previously, as well as isolates for which little or no ... | 1994 | 8259655 |