Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| detection of circulating antigen with a mab-based sandwich-elisa and its comparison with specific igm detection in sera of patients with hemorrhagic fever with renal syndrome. | a monoclonal antibody (1h2) against nucleocapsid protein of hantavirus was developed. a sandwich enzyme-linked immunosorbent assay (elisa) with the monoclonal antibody (mab) was established and evaluated for detecting circulating antigen (cag) in serum of patients with hemorrhagic fever with renal syndrome (hfrs). results were compared to that of an immunoglobulin m (igm)-detecting elisa. of 143 patients with hfrs, 106 were positive for cag of hantavirus and 128 positive for specific igm. among ... | 2007 | 17316085 |
| ex vivo stability of the rodent-borne hantaan virus in comparison to that of arthropod-borne members of the bunyaviridae family. | the possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. in this study we have compared the stabilities of three viruses within the bunyaviridae family: the rodent-borne hantavirus hantaan virus (htnv), the sand fly-borne phlebovirus sandfly fever sicilian virus (sfsv), and the tick-borne nairovirus crimean-congo hemorrhagic fever virus (cchfv). these viruses differ in their transmission routes: sfsv and cchfv are vector bo ... | 2007 | 17337567 |
| defining the n-linked glycosylation site of hantaan virus envelope glycoproteins essential for cell fusion. | the hantaan virus (htnv) is an enveloped virus that is capable of inducing low ph-dependent cell fusion. we molecularly cloned the viral glycoprotein (gp) and nucleocapsid (np) cdna of htnv and expressed them in vero e6 cells under the control of a cmv promoter. the viral gene expression was assessed using an indirect immunofluorescence assay and immunoprecipitation. the transfected vero e6 cells expressing gps, but not those expressing np, fused and formed a syncytium following exposure to a lo ... | 2007 | 17342054 |
| a comparative epidemiological study of hantavirus infection in japan and far east russia. | hantaviruses are causative agents of some severe human illnesses, including hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). the viruses are maintained by rodent hosts, and humans acquire infection by inhaling virus-contaminated excreta from infected animals. to examine the epidemiology of hantavirus infections in japan and far east russia, we conducted epidemiological surveys in these regions. in japan, anti-hantavirus antibodies were found in four rodent sp ... | 2007 | 17405352 |
| analysis of the immune response of hantaan virus nucleocapsid protein-specific cd8+ t cells in mice. | the major histocompatibility complex (mhc) class-i restricted epitope of hantaan virus nucleocapsid protein (n) was identified using overlapping peptides and balb/c mice. using the mhc tetramer derived from the epitope, we found that the level of n-specific cd8(+) t cells increased to approximately 20% of all antigen-specific cd8(+) t cells in a mouse model of transient infection. however, n-specific cd8(+) t cells were undetectable in a mouse model of persistent infection, both in the persisten ... | 2007 | 17481691 |
| mxa-independent inhibition of hantaan virus replication induced by type i and type ii interferon in vitro. | interferons (ifn) induce an antiviral state against hantaan virus (htnv) but the mechanisms responsible for inhibition are unclear. the ifn-inducible mxa is discussed to be important for control of infections with hantaviruses. to characterize the role of endogenous mxa, the inhibition of htnv induced by type i and type ii ifns was compared in vero and a549 cells. ifnalpha and ifngamma reduced production of infectious virions, viral rna, and nucleocapsid protein with the same efficiency, althoug ... | 2007 | 17482705 |
| [screening of biopolymers from sea hydrocoles affecting the adsorption of hantaan virus]. | the biopolymers extracted from sea hydrocoles were screened and a group of sulfated polysaccharides--fucoidans having a pronounced inhibitory effect on the adsorption of hantaan virus in the cultured vero e6 cells was identified. the mechanism of action of sulfated polysaccharides was realized through competitive carbohydrate-specific binding to cell membrane receptors and through ligand-receptor interaction and blocking of hantaan virus glycoproteins (g1 and g2). | 2007 | 17500236 |
| lambda interferon (ifn-lambda) in serum is decreased in hantavirus-infected patients, and in vitro-established infection is insensitive to treatment with all ifns and inhibits ifn-gamma-induced nitric oxide production. | hantaviruses, causing hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps), are known to be sensitive to nitric oxide (no) and to pretreatment with type i and ii interferons (alpha interferon [ifn-alpha]/ifn-beta and ifn-gamma, respectively). elevated serum levels of no and ifn-gamma have been observed in hfrs patients, but little is known regarding the systemic levels of other ifns and the possible effects of hantaviruses on innate antiviral immune respons ... | 2007 | 17522204 |
| nucleotide sequence characterization and phylogenetic analysis of hantaviruses isolated in shandong province, china. | china is the most severe endemic area of hemorrhagic fever with renal syndrome (hfrs) in the world with 30,000-50,000 cases reported annually, which accounts for more than 90% of total number of cases worldwide. the incidence rate of the syndrome in shandong province is one of the highest in china, which has ever reached 50 per 100,000 persons per year. however, the molecular characteristics of hantaviruses (hv) epidemic in shandong province remain unclear. therefore it is useful to clarify nucl ... | 2007 | 17531126 |
| dynein-dependent transport of the hantaan virus nucleocapsid protein to the endoplasmic reticulum-golgi intermediate compartment. | in contrast to most negative-stranded rna viruses, hantaviruses and other viruses in the family bunyaviridae mature intracellularly, deriving the virion envelope from the endoplasmic reticulum (er) or golgi compartment. while it is generally accepted that old world hantaviruses assemble and bud into the golgi compartment, some studies with new world hantaviruses have raised the possibility of maturation at the plasma membrane as well. overall, the steps leading to virion assembly remain largely ... | 2007 | 17537852 |
| [prevalence of antibodies to hantavirus among hemodialysis patients with end-stage renal failure in kaunas and its district]. | the objective of this study was to investigate the prevalence of antibodies to hantaviruses among hemodialysis patients with end-stage renal failure in kaunas and its district. | 2007 | 17551280 |
| envelope glycoproteins of hantavirus can mediate cell-cell fusion independently. | hantaviruses (htvs) are enveloped viruses and can induce low ph-dependent cell fusion. in this report we molecularly cloned viral glycoproteins (gps) cdna and nucleocapsid (np) cdna of two strains of hantaan virus and one strain of seoul virus and expressed in vero e6 cells under control of a cmv promoter. the examinations of viral gene expressions were carried out by ifa and immune-precipitation. after treatment with low ph (ph 5.8) medium the syncytium were observed in the cells transfected wi ... | 2007 | 17619252 |
| a new hantaan-like virus in rodents (apodemus peninsulae) from northeastern china. | lung tissue samples of 76 korean field mice (apodemus peninsulae) collected from northeastern china bordering with far east russia and korea were detected for hantavirus partial m-segment or entire s-segment sequences by rt-pcr and 481-nt mitochondrial dna fragment of the rodents. four a. peninsulae mice were found positive for partial m-segment of hantavirus. sequence analyses of partial m-segment or/and entire s-segment of the hantaviruses revealed that three were closely related to hantaan vi ... | 2007 | 17624464 |
| [late consequences of hemorrhagic fever with renal syndrome in a woman who had it during pregnancy]. | the authors adduce a brief description of the features and outcomes of hemorrhagic fever with renal syndrome (hfrs) in 8 pregnant women. the results of the examination of a 54-year-old woman and her son, who suffered from a severe form of hers 28 years ago during the 31st week of pregnancy, are presented in detail. antibodies to hantaan virus 1:32 were found; magnetic resonance tomography of the skull revealed sequelae of hypophysial hemorrhage with the formation of "partly empty ephippium". the ... | 2007 | 17665611 |
| ribavirin reveals a lethal threshold of allowable mutation frequency for hantaan virus. | the broad spectrum of antiviral activity of ribavirin (rbv) lies in its ability to inhibit imp dehydrogenase, which lowers cellular gtp. however, rbv can act as a potent mutagen for some rna viruses. previously we have shown a lack of correlation between antiviral activity and gtp repression for hantaan virus (htnv) and evidence for rbv's ability to promote error-prone replication. to further explore the mechanism of rbv, gtp levels, specific infectivity, and/or mutation frequency was measured i ... | 2007 | 17699579 |
| detection and typing of human pathogenic hantaviruses by real-time reverse transcription-pcr and pyrosequencing. | because the clinical course of human infections with hantaviruses can vary from subclinical to fatal, rapid and reliable detection of hantaviruses is essential. to date, the diagnosis of hantavirus infection is based mainly on serologic assays, and the detection of hantaviral rna by the commonly used reverse transcription (rt)-pcr is difficult because of high sequence diversity of hantaviruses and low viral loads in clinical specimens. | 2007 | 17717126 |
| [the in vitro antiviral activity against the causative agent of hemorrhagic fever with renal syndrome]. | it is shown that primary screening of drugs against the pathogens of hemorrhagic fever with renal syndrome (hfv) may be performed, by using two approaches in estimating the suppression of plague formation and that of viral reproduction in the cultured cells. it is expedient to make a primary screening of interferon and its inductors to estimate the suppression of viral reproduction in the cultured vero e6, psev, and cl-17 cells, the infection multiplicity should be hundredth parts of boe/cell. a ... | 2007 | 17722609 |
| genetic properties of medium (m) and small (s) genomic rna segments of seoul hantavirus isolated from rattus norvegicus and antigenicity analysis of recombinant nucleocapsid protein. | a novel isolate of seoul (seo) hantaviruses was detected and identified in rattus norvegicus in shandong province, china and designated as jun5-14. the partial m segment and the coding region of nucleocapsid protein (np) in the s segment of jun5-14 were pcr-amplified and sequenced. nucleotide sequence analysis of the partial m segment (300 bp) revealed that jun5-14 isolate was closely related to former seo isolates in shandong (97.3-99.0% homology) and non-shandong seo viruses (84.1-97.7% homolo ... | 2007 | 16927115 |
| clinical and laboratory predictors of oliguric renal failure in haemorrhagic fever with renal syndrome caused by hantaan virus. | haemorrhagic fever with renal syndrome (hfrs), caused by hantavirus infection, develops into acute renal failure (arf) of variable degrees of severity. we investigated the early predictive markers for oliguric arf in hfrs patients. | 2007 | 16930718 |
| hsp70 gene fused with hantavirus s segment dna significantly enhances the dna vaccine potency against hantaviral nucleocapsid protein in vivo. | heat shock proteins (hsps) have been shown to act as adjuvants when coadministered with peptide antigens or given as fusion proteins and enhance the vaccination efficiency. to evaluate the enhancement of the potency of hantaan virus (htnv) nucleocapsid protein (np) immunogenicity by heat shock protein 70 (hsp70), we developed a novel chimeric htnv s-hsp70 dna vaccine plasmid by genetically linking hsp70 gene to the full-length htnv s segment dna (htnv s dna). c57bl/6 mice were immunized with thi ... | 2007 | 16935395 |
| a soluble and heat-resistant form of hantavirus nucleocapsid protein for the serodiagnosis of hfrs. | hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne viral zoonosis characterized by fever, hemorrhagic manifestations, and renal disorder. the causative agent of hfrs has been identified as a hantavirus. hantavirus nucleocapsid proteins have recently been shown to be immunodominant antigens in hfrs, inducing an early and long-lasting immune response, and their amino termini are sensitive tools for the detection of specific antibodies in hfrs patient sera. previous work has demonstrate ... | 2008 | 17826847 |
| heat shock protein 70 fused to or complexed with hantavirus nucleocapsid protein significantly enhances specific humoral and cellular immune responses in c57bl/6 mice. | heat shock proteins (hsps) are known to act as an effective molecular adjuvant to enhance the induction of antigen peptide-specific cellular immunity, when coupled with the antigen or peptide. hantaan virus (htnv) nucleocapsid protein (np) is relatively conserved among hantaviruses and highly immunogenic in both animals and humans. to analyze the influence of hsp70 on np vaccine potency, and evaluate the possibility of developing a novel effective vaccine against hantaviruses, we constructed pro ... | 2008 | 18479786 |
| mixing of m segment dna vaccines to hantaan virus and puumala virus reduces their immunogenicity in hamsters. | to determine if dna vaccines for two hantaviruses causing hemorrhagic fever with renal syndrome, hantaan virus and puumala virus, are immunogenic when given in combination, we delivered them to hamsters separately or as mixtures by gene gun or by electroporation. both vaccines elicited neutralizing antibodies when given alone but when they were delivered as a mixture, antibodies to only one of the two hantaviruses could be detected. in contrast, if the dnas were given as separate vaccinations to ... | 2008 | 18482782 |
| [3 cases of hemorrhagic fever with renal syndrome morbidity]. | viral origin of hemorrhagic fever with renal syndrome (hfrs) is known since 40th years of last century. also it is known that contamination of person is performed mainly by air pollution way. we observed a focus with 3 hfrs cases (with laboratory confirmation--antibodies to hfrs hantavirus were found) in one family with one lethal outcome. contamination of patients occurred by air pollution way during stocking vegetables contaminated with rodent's excrements. lethal outcome was stipulated by inc ... | 2008 | 18494292 |
| [inhibiting effects of fucoidans on hantaan virus adsorption on a model of peritoneal macrophages in vitro]. | a model of peritoneal macrophages was used to study the effect of fucoidans from brown seaweeds on the adsorption of hantaan virus. fucoidans were found to have antiviral activity, but to differ in the inhibition of hantavirus adsorption, which was associated with their structural features. the mechanism of their action is assumed to be shown via ligand-receptor interaction with certain cell membrane receptors and via blockage of hantavirus glycoproteins (g1 and g2), resulting in adsorption inhi ... | 2008 | 18590128 |
| [related substitutions in the small segment of old world hantavirus genome]. | whether there were related nucleotide substitutions in the s-segments of the genome of tula, puumala, hantaan, dorava/belgrad, and seoul viruses was studied. the significance of the correlations found in nucleotide substitutions was estimated by the level of mutual information. the majority of the correlated changes were synonymous substitutions. the correlations were mostly restricted to non-synonymous substitutions sites: tula 258 (a.a.) and dobrava/belgrad (56 a.a.). in the genome of puumala, ... | 2008 | 18590133 |
| lack of vertical transmission of hantaan virus from persistently infected dam to progeny in laboratory mice. | it is unclear how the hantaviruses are transferred from infected to uninfected rodents. we studied the status of persistently infected laboratory mice and examined the frequency of viral transmission to their offspring. expression of hantaan virus nucleocapsid protein was detected in the lungs of persistently infected dams. none of the progeny displayed viral antigen, although they were strongly positive for igg antibodies against hantavirus. there was neither hantavirus rna nor virus-specific i ... | 2008 | 18612586 |
| molecular diversity and phylogeny of hantaan virus in guizhou, china: evidence for guizhou as a radiation center of the present hantaan virus. | to gain further insight into the molecular epidemiology of hantaan virus (htnv) in guizhou, china, rodents were captured in this region in 2004 and 2005. in addition, serum samples were collected from four patients. ten hantaviruses were isolated successfully in cell culture from four humans, two apodemus agrarius, three rattus norvegicus and one rattus nitidus. the nucleotide sequences for their small (s), medium (m) and partial large (l) segments were determined. phylogenetic analysis of the s ... | 2008 | 18632971 |
| hantaan virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon, interleukin-6 and tumor necrosis factor-alpha. | hantaan virus (htnv) infects endothelial cells and is associated with increased vascular permeability during hemorrhagic fever with renal syndrome (hfrs). the pattern of increased vascular permeability is mediated by immune response. therefore, it is necessary to characterize the mechanism of htnv involvement in the host's innate immune. in this study, the expression of five toll-like receptors (tlrs) was analyzed in endothelial vein cells (evc-304) following htnv infection in vitro. tlr4 showed ... | 2008 | 18707748 |
| serum thrombospondin-1 is altered in patients with hemorrhagic fever with renal syndrome. | hemorrhagic fever with renal syndrome (hfrs) is a severe acute viral disease with pathological changes of impaired capillary and small vessels and thrombocytopenia. in this study, serum thrombospondin (tsp)-1 concentration in patients with hfrs was determined to explore its possible role in the pathogenesis of hfrs. the concentration of tsp-1 was measured using a competitive enzyme-linked immunoabsorbent assay. significantly decreased levels of serum tsp-1 were observed in hfrs patients at febri ... | 2008 | 18712840 |
| expression of a hantavirus n protein and its efficacy as antigen in immune assays. | hantavirus cardiopulmonary syndrome (hcps) has been recognized as an important public heath problem. five hantaviruses associated with hcps are currently known in brazil: juquitiba, araraquara, laguna negra-like, castelo dos sonhos, and anajatuba viruses. the laboratory diagnosis of hcps is routinely carried out by the detection of anti-hantavirus igm and/or igg antibodies. the present study describes the expression of the n protein of a hantavirus detected in the blood sample of an hcps patient ... | 2008 | 18719741 |
| situation of hantavirus infections and haemorrhagic fever with renal syndrome in european countries as of december 2006. | hantavirus infections are widely distributed in europe with the exception of the far north and the mediterranean regions. the underlying causes of varying epidemiological patterns differ among regions: in western and central europe epidemics of haemorrhagic fever with renal syndrome (hfrs) caused by hantavirus infections follow mast years with increased seed production by oak and beech trees followed by increased rodent reproduction. in the northern regions, hantavirus infections and hfrs epidem ... | 2008 | 18761927 |
| hemorrhagic fever with renal syndrome in montenegro. | the objective of the study was to analyze the epidemiological features of hemorrhagic fever with renal syndrome (hfrs) in montenegro. the study included 169 cases of hfrs diagnosed in the period between 1995 and 2005 according to the clinical symptoms and serological confirmation. for the analysis of the demographic characteristics of the cases, as well as of the chronological and topographical features of the disease, a descriptive epidemiological method was employed. the average incidence rate ... | 2008 | 18806348 |
| a hantavirus causing hemorrhagic fever with renal syndrome requires gc1qr/p32 for efficient cell binding and infection. | hantaan virus (htnv) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). htnv infection is mediated by alpha v beta3 integrin. we used protein blots of vero e6 cell homogenates to demonstrate that radiolabeled htnv virions bind to gc1qr/p32, the acidic 32-kda protein known as the receptor for the globular head domain of complement c1q. rnai-mediated suppression of gc1qr/p32 markedly reduced htnv binding and infection in human lung epithelial a549 cells. conversel ... | 2008 | 18834607 |
| [participation of murine rodents in circulation of agents of tularemia and hemorrhagic fever in kola peninsula]. | results of virological and bacteriological studies of wild mammals of 11 species from rodentia and cricetidae genuses during epizootic period (spring-autumn 2006-2007) in murmansk region are presented. the number of red-baked mice (clethrionomys) and common vole (microtus) was rising. antigen of hemorrhagic fever with renal syndrome virus as well as tularemia pathogen were found in background rodent species. | 2008 | 19004288 |
| [the reactivity of sera from hemorrhagic fever in patients with renal syndromes to the recombination nucleotide proteins from european hantaviruses in hubei province]. | five yeast-expressed recombination nucleotide proteins of european hantaviruses were prepared as coated antigens to detect hantavirus-specific antibodies in sera from hemorrhagic fever with renal syndromes (hfrs) in hubei province, through elisa assay. the relativity among hantaviruses prevailing in different areas was investigated. | 2008 | 19040041 |
| antiviral effect of human saliva against hantavirus. | hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome are zoonotic diseases caused by rodent borne hantaviruses. transmission to humans occurs usually by inhalation of aerozolized virus-contaminated rodent excreta. although human-to-human transmission of andes hantavirus has been observed, the mode of transmission is currently not known. saliva from puumala hantavirus (puuv)-infected patients was shown recently to contain viral rna. to test if human saliva interferes wit ... | 2008 | 19040288 |
| [simulation of the disease caused by hantaan virus in white mice by a complex of virological, biochemical, and hematological parameters]. | the essence of studies was that the disease is simulated in 12-day albino mice subcutaneously infected with hantaan virus, strain 76-118 in a dose of 10 ld50. as an efficiency index, the study of drugs uses major (death protection coefficient, mean animal lifetime) and auxiliary (virological: pathogen accumulation in the brain tissues of deceased animals) parameters, biochemical (the levels of creatinine, urea, alanine aminotransferase, aspartate aminotransferase, malonic dialdehyde), hematologi ... | 2008 | 19069793 |
| ecological surveillance of small mammals at firing points 10 and 60, gyeonggi province, republic of korea, 2001-2005. | throughout korea, small mammals are hosts to a number of disease-causing agents that pose a health threat to u.s. and korean military forces while they conduct field-training exercises. a seasonal rodent-borne disease surveillance program was established at two firing points (fp), fp-10, and fp-60, and conducted over five years from 2001 through 2005 in response to hantavirus cases among u.s. soldiers. the ecology of these sites consisted primarily of tall grasses associated with semi-permanent ... | 2008 | 19263858 |
| [transformation of full-length gene of mouse/human chimeric antibody against hantaan virus into arabidopsis thaliana]. | to construct the transgenic arabidopsis thaliana containing full-length gene of mouse/human chimeric antibody(3g1mh) against hantaan virus. | 2008 | 18177619 |
| new and old world hantaviruses differentially utilize host cytoskeletal components during their life cycles. | recently we reported that the n protein of the old world hantavirus, hantaan (htnv), traffics on microtubules to the er-golgi intermediate compartment (ergic) prior to its movement to the golgi and requires an intact ergic for viral replication. we have extended these studies to the new world hantaviruses, andes virus (andv) and black creek canal virus (bccv), and an additional old world hantavirus, seoul virus (seov). these studies support microtubule-dependent trafficking of the n protein to e ... | 2008 | 18234268 |
| development and evaluation of serological assays for detection of hantaanvirus-specific antibodies in human sera using yeast-expressed nucleocapsid protein. | indirect and capture enzyme-linked immunosorbent assays (elisas) for detection of hantaan virus (htnv)-specific immunoglobulins g (igg) and m (igm) in human serum samples were developed on the basis of recombinant yeast-expressed nucleocapsid (n) protein of htnv. the sensitivities and specificities of the indirect and capture elisas were evaluated by comparing the reactivity of sera from patients with hemorrhagic fever with renal syndrome (hfrs) from china with that of a commercial igg/igm kit. ... | 2008 | 18077007 |
| [discovery, clinical and etiological characteristic of hemorrhagic fever with renal syndrome in the subtropical zone of krasnodar region]. | twenty-six patients with hemorrhagic fever with renal syndrome (hfrs) were revealed as a result of serological examination of 582 patients with fever living around sochi town. etiologic role of dobrava virus subtype as the cause of hfrs was assessed by immunofluorescent and elisa assays, and neutralization test. the principal host of this virus and source of infection for humans is caucasian forest mouse apodemus ponticus. hfrs morbidity was sporadic and not dependent from patients' occupation a ... | 2008 | 18376466 |
| synthesis of 1-beta-d-ribofuranosyl-3-ethynyl-[1,2,4]triazole and its in vitro and in vivo efficacy against hantavirus. | there are no fda approved drugs for the treatment of hemorrhagic fever with renal syndrome (hfrs), a serious human illnesses caused by hantaviruses. clinical studies using ribavirin (rbv) to treat hfrs patients suggest that it provides an improved prognosis when given early in the course of disease. given the unique antiviral activity of rbv and the lack of other lead scaffolds, we prepared a diverse series of 3-substituted 1,2,4-triazole-beta-ribosides and identified one with antiviral activity ... | 2008 | 18394724 |
| intensity of platelet beta(3) integrin in patients with hemorrhagic fever with renal syndrome and its correlation with disease severity. | beta(3) integrin has been identified as a cellular receptor for hantaan virus, which causes hemorrhagic fever with renal syndrome (hfrs). to investigate the relationship between intensity of the platelet membrane beta(3) integrin (cd61) and disease severity, the percentage of cd61-positive platelets and the mean fluorescence intensities (mfi) of platelet cd61 were determined in patients with hfrs by flow cytometry. the intensity levels of cd61 in patients with hfrs were significantly higher than ... | 2008 | 18419255 |
| genetic characterization of hantaviruses isolated from guizhou, china: evidence for spillover and reassortment in nature. | to gain more insights into the epidemiology of hantaviruses in the guizhou province, china, rodents were captured in guizhou during the period from 2001 to 2003. in addition, serum sample was collected from one patient. virus isolation was attempted from human serum and rodent samples. four hantaviruses were isolated successfully in cell culture from one human, two a. agrarius, and one r. norvegicus. the nucleotide sequences for the entire s and m and partial l segment were determined from these ... | 2008 | 18428127 |
| processing of genome 5' termini as a strategy of negative-strand rna viruses to avoid rig-i-dependent interferon induction. | innate immunity is critically dependent on the rapid production of interferon in response to intruding viruses. the intracellular pathogen recognition receptors rig-i and mda5 are essential for interferon induction by viral rnas containing 5' triphosphates or double-stranded structures, respectively. viruses with a negative-stranded rna genome are an important group of pathogens causing emerging and re-emerging diseases. we investigated the ability of genomic rnas from substantial representative ... | 2008 | 18446221 |
| real-time rt-pcr of hantaan virus rna used for the detection of virus response to antiviral drugs. | hantaan virus (htn) is an important cause of hemorrhagic fever with renal syndrome (hfrs) in korea. htn rna can be detected with the rt-pcr and the quantity of htn rna in infected cells can be measured by competitive rt-pcr. the current study used the real-time rt-pcr for the detection of viral rna s gene in a more detailed fashion than in the previous study (nam et al., virus genes 26, 31-38, 2003). a standard curve was generated with serial 10-fold dilutions of the htn rna. the sensitivity of ... | 2008 | 18459839 |
| hantavirus causing hemorrhagic fever with renal syndrome enters from the apical surface and requires decay-accelerating factor (daf/cd55). | the old world hantaviruses, members of the family bunyaviridae, cause hemorrhagic fever with renal syndrome (hfrs). transmission to humans occurs via inhalation of aerosols contaminated with the excreta of infected rodents. the viral antigen is detectable in dendritic cells, macrophages, lymphocytes, and, most importantly, microvascular endothelial cells. however, the site and detailed mechanism of entry of hfrs-causing hantaviruses in polarized epithelial cells have not yet been defined. theref ... | 2008 | 18305044 |
| seroepidemiological study on hantavirus infections in india. | hantaviruses are etiological agents of hemorrhagic fever with renal syndrome in many parts of asia and europe. there has been no documented case of hantavirus disease from india, although serological evidence exists. we investigated the prevalence of hantavirus in the indian population and tried to identify potential risk groups for hantavirus infections. the presence of hantavirus-specific igg antibodies was prospectively evaluated in 661 subjects belonging to different groups, i.e. patients wi ... | 2008 | 17996913 |
| expression of l protein of hantaan virus 84fli strain and its application for recovery of minigenomes. | hantaan virus (htnv) is an old world hantavirus associated with hemorrhagic fever with renal syndrome (hfrs). to visualize the localization of the l protein of htnv strain 84fli within cells, a fusion protein composed of enhanced green fluorescent protein and l protein, egfp-l, was expressed in vero cells. the 273 kda expressed fusion protein of egfp-l localized in the perinuclear region. we also described the development of a reverse genetics system for htnv strain 84fli. the rna polymerase i ( ... | 2008 | 19133012 |
| immunological properties of a fusion protein containing nucleocapsid protein and glycoprotein gn of hantaan virus. | escherichia coli and the baculovirus bac-to-bac system were used to express chimeric gene gns0.7 consisting of glycoprotein gn gene and the 0.7 kb fragment of s genome segment carrying nucleoprotein (n) gene of hantaan virus (htnv). the expressed fusion protein gnn0.7 was recognized by monoclonal antibodies (mabs) to htnv gn glycoprotein and n protein, respectively. its molecular mass as determined by western blot analysis corresponded to the predicted value. the mice immunized with insect spodo ... | 2008 | 19143481 |
| genetic and antigenic analyses of a puumala virus isolate as a potential vaccine strain. | puumala virus (puuv), a causative agent of hemorrhagic fever with renal syndrome (hfrs), is prevalent in europe and european russia. no vaccine has been developed for puuv-associated hfrs, primarily because of the low viral yield in cultured cells. a puuv strain known as dtk/ufa-97 was isolated in russia and adapted for growth in vero e6 cells maintained in serum-free medium. the dtk/ufa-97 strain produced a higher viral titer in serum-free medium, suggesting that it may prove useful in the deve ... | 2008 | 19177744 |
| hantaviruses: an emerging public health threat in india? a review. | the emerging viral diseases haemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps) are a cause of global concern as they are increasingly reported from newer regions of the world. the hantavirus species causing hfrs include hantaan virus,seoul virus, puumala virus, and dobrava-belgrade virus while sin nombre virus was responsible for the 1993 outbreak of hcps in the four corners region of the us. humans are accidental hosts and get infected by aerosols gener ... | 2008 | 19208975 |
| [detection of anti-hantavirus antibodies in forest workers in the north-east of poland]. | to estimate exposition to hantaviruses in a high-risk population (forest workers) in the north-east of poland. | 2008 | 19108515 |
| [application of epstein-barr virus-transformed b lymphoblastic cells in identification of ctl epitopes specific for hantaan virus]. | to establish epstein-barr virus (ebv)-transformed b lymphoblastic cell lines (b-lcl) to present peptides as antigen-presenting cells (apc) and stimulate short-cultured t cells secreting ifn-gamma, by which the t cell epitopes are identified. | 2009 | 19126382 |
| collective immunity of the population from endemic zones of hemorrhagic fever with renal syndrome in kosovo. | hemorrhagic fever with renal syndrome (hfrs), also known as mice fever is an acute viral zoonosis and it appears in the natural focus after the human contact with hantaan virus infected mice. the objective (purpose) of this study was to investigate the prevalence of specific antibodies in hfrs, in convalescent persons (collective immunity in endemic hearths). in this project we applied the epidemiological method of studying with retrospective-perspective, the serological method for determination ... | 2009 | 20088164 |
| [construction of a recombinant adenovirus carrying the hantaan virus glycoprotein g2 gene and its expression in hela cells]. | we constructed the recombinant adenovirus expressing the glycoprotein g2 of hantaan virus. firstly we obtained coding gene fragment of g2 by pcr, and subsequently inserted the gene of interest into the adenoviral pshuttle vector pad5-cmv. then we co-transfected the recombinant pshuttle vector and adenovirus skeleton plasmid into hek293 cells by calcium phosphate precipitation method. after the recombinant adenovirus were packaged and amplified in hek293 cells, we observed the expression of repor ... | 2009 | 20112705 |
| hantaan virus triggers tlr3-dependent innate immune responses. | immediately after viral infection, innate responses including expression of ifn-alpha/beta and ifn-stimulated genes (isgs) are elicited ubiquitously by recruitment of specific pathogen recognition receptors. the velocity to induce ifn-alpha/beta and isgs in response to an infection is often decisive for virulence. interestingly, in primary endothelial cells isgs are induced later by hantaviruses pathogenic to humans than those considered to be nonpathogenic or of low virulence. here we demonstra ... | 2009 | 19234180 |
| acute febrile illness caused by hantavirus: serological and molecular evidence from india. | study of hantavirus infections in india is in its early stages. as early symptoms of hantavirus disease can be non-specific and the diagnosis confirmed only by laboratory testing, use of appropriate diagnostic tools is important. to improve the diagnosis of hantavirus infections in india, commercial elisa systems followed by indirect immunofluorescence assays were used to detect anti-hantavirus igm and igg in samples from patients with acute febrile illness. of 347 patients tested, 5.2% showed s ... | 2009 | 19237179 |
| [application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome]. | to establish a flow microbeads assay (fma) to examine the level of hemorrhagic fever with renal syndrome virus (hfrs v.) specific igm, igg and cytokines in hfrs patients. | 2009 | 19257990 |
| hantavirus species in india: a retrospective study. | hantaviruses cause hemorrhagic fever with renal syndrome in europe and asia. there are about 20 documented hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. focus reduction neutralization test is the classical serotyping technique for hantavirus. however, this study employs a previously established serotyping elisa, to retrospectively analyze known hantavirus igg reactive samples for infecting serotypes. the result suggests prese ... | 2009 | 19736405 |
| the interferon response circuit in antiviral host defense. | viruses have learned to multiply in the face of a powerful innate and adaptive immune response of the host. they have evolved multiple strategies to evade the interferon (ifn) system which would otherwise limit virus growth at an early stage of infection. ifns induce the synthesis of a range of antiviral proteins which serve as cell-autonomous intrinsic restriction factors. for example, the dynamin-like mxa gtpase inhibits the multiplication of influenza and bunyaviruses (such as la crosse virus ... | 2009 | 19739399 |
| [serological characteristics of hantaviruses from clinical specimens analyzed in 1998-2008 in the department of virology, public health institute, ostrava]. | in 1998 - 2008, serum samples from 464 patients, predominantly residents of the moravia-silesian region, were examined in the department of virology, public health institute in ostrava, using enzyme-linked immunosorbent assay (elisa) for the detection of specific anti-hantavirus antibodies. seropositivity was ascertained in 41 persons (8.8%). more patients tested seropositive against hantaan virus (24, i.e. 58.5%) than against puumala virus (13, i.e. 31.7%). four persons were reactive against bo ... | 2009 | 19750821 |
| inhibition of tnf-alpha-induced activation of nf-kappab by hantavirus nucleocapsid proteins. | hantaviruses cause two human diseases thought to be immune-mediated: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). we recently reported that the nucleocapsid (n) protein of hfrs-causing hantaan virus (htnv) inhibits tumor necrosis factor-alpha (tnf-alpha) activation of nuclear factor-kappab (nf-kappab). here we measured the ability of other hantaviral n proteins to similarly interfere with the inflammatory process of tnf-alpha. we found that like htnv n, t ... | 2009 | 19751407 |
| phase i evaluation of the safety and pharmacokinetics of a single-dose intravenous injection of a murine monoclonal antibody against hantaan virus in healthy volunteers. | anti-hantaan virus monoclonal antibody (ahm) is a murine monoclonal antibody against hantaan virus being developed for the treatment of hemorrhagic fever with renal syndrome. the purpose of the present study was to describe the tolerance and pharmacokinetics of an intravenously administered single ascending dose of ahm in chinese healthy volunteers. four cohorts of 22 healthy subjects received ahm at 2.5 to 20 mg, and the results indicated that ahm was well tolerated. we established a highly sen ... | 2009 | 19752279 |
| identification of oligopeptides mimicking the receptor-binding domain of hantaan virus envelope glycoprotein from a phage-displayed peptide library. | attachment of enveloped viruses to cells is triggered by the receptor-binding domain (rbd) on envelope glycoproteins (gp) binding to receptors located on the cell surface. to date, recognized receptors and rbd of hantaan virus (htnv) have not been exactly defined. in this study, one monoclonal antibody (mab) 3g1 possessing high neutralizing activity, which is directed against htnv envelope glycoprotein g2, was used to determine the crucial motif of rbd. peptide ligands binding to mab 3g1 were se ... | 2009 | 19767836 |
| hantavirus infection in the republic of georgia. | we describe a laboratory-confirmed case of hantavirus infection in the republic of georgia. limited information is available about hantavirus infections in the caucasus, although the infection has been reported throughout europe and russia. increasing awareness and active disease surveillance contribute to our improved understanding of the geographic range of this pathogen. | 2009 | 19788822 |
| co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary. | hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ... | 2009 | 19856478 |
| hemorrhagic fever with renal syndrome in 4 us soldiers, south korea, 2005. | four us soldiers acquired hemorrhagic fever with renal syndrome while training near the demilitarized zone, south korea, in 2005. hantaan virus sequences were amplified by reverse transcription-pcr from patient serum samples and from lung tissues of striped field mice (apodemus agrarius) captured at training sites. epidemiologic investigations specified the ecology of possible sites of patient infection. | 2009 | 19891878 |
| hsp70 induced by hantavirus infection interacts with viral nucleocapsid protein and its overexpression suppresses virus infection in vero e6 cells. | hantavirus (htv) infection is known to induce innate cellular response, a more specified cellular response in the host cells. however, whether it stimulates synthesis of stress proteins, particularly associations of viral proteins, is entirely unknown. the primary focus of this research is using vero e6 cells infected with hantaan 76-118 (htnv) as an in vitro infection model to examine the individual contribution of htv infection to heat shock response. this study shows that htnv infection rapid ... | 2009 | 19956449 |
| [comparison on the difference of virulence between hantaan virus and seoul virus isolated both from rattus norvegicus]. | comparing the difference of virulence between the strain cgrn5310 (htnv) and the strain hr54 (seov) isolated both from rattus norvegicus. | 2009 | 20193387 |
| [complete sequence of the m segment of amur virus in rodent from china]. | to study the complete sequence of m segment of amur virus in rodents and to explore their molecular characteristics. | 2009 | 20193388 |
| [association of hemorrhagic fever with renal syndrome and hla-drb allele polymorphisms in han nationality, hubei province.]. | to understand the association of human leukocyte antigen (hla)-drb polymorphism and patients diagnosed as hemorrhagic fever with renal syndrome (hfrs). | 2009 | 20193583 |
| ecology of hantaan virus at twin bridges training area, gyeonggi province, republic of korea, 2005-2007. | the twin bridges training area (tbta) in the republic of korea consists of dirt roads, barren training areas, and forested hillsides adjacent to linear and broad expanses of tall grasses, herbaceous, and scrub vegetation. of the six species of small mammals, the striped field mouse, apodemus agrarius, was the most frequently captured (96.1%). apodemus agrarius capture rates varied from 17.7 to 33.2% during three trapping periods. gravid females were observed during november-december 2006 (8.4%) ... | 2009 | 20836826 |
| diagnostic aspects for epidemic hemorrhagic fever in legal medical autopsy: report of 2 cases and review. | epidemic hemorrhagic fever (ehf) is known also as hemorrhagic fever with renal syndrome (hfrs). hantaviruses, a notorious cause of ehf is spreaded by rodents or biting insects, can cause epidemic hemorrhagic fever. this report describes two rare medico-legal autopsy cases died from epidemic hemorrhagic fever. the victims were two male individuals aged 34 years and 29 years. the medico-legal autopsies were requested for alleged medical negligence. in both cases, autopsy reveals lesions to kidneys ... | 2009 | 19269214 |
| a case of graves' disease combined with hantaan virus infection. | graves' disease (gd) is generally presented by thyrotoxicosis with hyperthyroidism, and it is an organ-specific autoimmune disease induced by thyroid-stimulating hormone receptor autoantibodies. however, among diverse etiologies, viral infections have been suggested to trigger or to be involved in the pathogenesis of gd. hantaan virus infection causing hemorrhagic fever with renal syndrome (hfrs) is common in south korea and its pathogenesis is suggested to be an immunologic mechanism. we have e ... | 2009 | 19270831 |
| reduced expression of angiopoietin-1 in hantaan virus-infected human umbilical vein endothelial cell increases their permeability. | hemorrhagic fever with renal syndrome (hfrs) caused by hantaan virus (htnv) is characterized by vascular hemorrhage and acute renal failure. angiopoietin-1 (ang-1) is a glycoprotein that maintains vessel integrity and reduces endothelial permeability. we found that in htnv-infected human umbilical vein endothelial cells (huvecs) the levels of ang-1 mrna and protein were reduced on days 2 and 3 post-infection (p.i.), when endothelial permeability was increased. the htnv-stimulated permeability wa ... | 2009 | 19301945 |
| adenovirus vectors expressing hantavirus proteins protect hamsters against lethal challenge with andes virus. | hantaviruses infect humans following aerosolization from rodent feces and urine, producing hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. due to the high rates of mortality and lack of therapies, vaccines are urgently needed. nonreplicating adenovirus (ad) vectors that express andes hantavirus (andv) nucleocapsid protein (adn) or glycoproteins (adg(n) and adg(c)) were constructed. ad vectors were tested for their ability to protect syrian hamsters from a lethal andv inf ... | 2009 | 19403663 |
| low levels of serum vitronectin associated with clinical phases in patients with hemorrhagic fever with renal syndrome. | beta(3) integrin has been identified as a cellular receptor for hantaan virus which causes hemorrhagic fever with renal syndrome (hfrs). as one of the ligands of beta(3) integrin, vitronectin (vn) may be altered in hfrs. in this study, changes of serum vn levels were determined in 112 patients with hfrs and 30 age- and sex-matched healthy controls by quantitative sandwich enzyme immunoassay. the levels of serum vn were analyzed in patients at various phases of hfrs and with different severity of ... | 2009 | 19408099 |
| hantavirus pulmonary syndrome: a concise clinical review. | in 1978, hantaviruses were first described as the etiological agent of hemorrhagic fever with renal syndrome (hfrs) in korea. since then, numerous related, enveloped, negative-stranded rna viruses have been identified, forming the genus hantavirus within the family bunyaviridae. these pathogens are distributed worldwide and thus can be classified, on the basis of phylogenetic origins, into old world viruses or new world viruses (ie north, central, and south america). similarly, these viruses cau ... | 2009 | 19434035 |
| gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein. | a functionalized gold nanoparticle (gnp) enhanced ultrasensitive immuno-pcr assay (gnp-ipcr on elisa plate), which was modified from the recent developed bio-barcode assay (bca) technique, was developed to detect hantaan virus nucleocapsid protein (hnp). during the assay, the target antigen hnp was captured by a polyclonal antibody coated on elisa microplate wells, followed by adding gnp dually modified with oligonucleotides and a hnp specific monoclonal antibody l13 (mab l13) to form a sandwich ... | 2009 | 19467237 |
| genetic susceptibility to haemorrhagic fever with renal syndrome caused by hantaan virus in chinese han population. | we report a significantly higher occurrence of hla-drb1*09 (51% vs. 23%, p = 0.002, or = 3.57) and hla-b*46-drb1*09 (26% vs. 8%, p = 0.018, or = 3.76) in patients with haemorrhagic fever with renal syndrome (hfrs) compared to the control group, suggesting hantaan virus-induced hfrs is associated with a genetic predisposition in the chinese han population. | 2009 | 19473214 |
| synthesis and anti-hantaan virus activity of n(1)-3-fluorophenyl-inosine. | as part of an ongoing effort to develop new antiviral nucleoside analogs, our interest was drawn to n(1)-aryl purines as a novel structural class and potential scaffold for drug discovery. herein, we describe the synthesis of n(1)-3-fluorophenyl-inosine (fpi) and n(1)-3-fluorophenyl-hypoxanthine (fp-hx) and their antiviral activity against hantaviruses. the ec(50) for fpi and fp-hx were 94 and 234microm, respectively, against hantaan virus. fpi was not toxic to mammalian cells at concentrations ... | 2009 | 19501259 |
| hantaviruses in rodents and humans, inner mongolia autonomous region, china. | surveys were carried out in 2003-2006 to better understand the epidemiology of hantaviruses in the inner mongolia autonomous region of china (inner mongolia). hemorrhagic fever with renal syndrome (hfrs) was first reported in this region in 1955 and has been an important public health problem here since then. during 1955-2006, 8,309 persons with hfrs were reported in inner mongolia (average incidence rate 0.89/100,000), and 261 (3.14%) died. before the 1990s, all hfrs cases occurred in northeast ... | 2009 | 19523286 |
| genotyping of hantaviruses occurring in linyi, china, by nested rt-pcr combined with single-strand conformation polymorphism analysis. | the hemorrhagic fever with renal syndrome (hfrs) incidence rate still holds in high level in some areas of the world, but a reliable and simple typing method that can be used in clinical diagnosis and epidemiologic surveys is not available. in this study, 48 serum samples were collected from patients with hfrs in linyi area, china, that is seriously affected by this disease. the collected samples were analyzed by nested rt-pcr combined with single-strand conformation polymorphism (sscp) for geno ... | 2009 | 19537913 |
| [study on the difference of genes and the type identification of hantavirus from lishui, zhejiang province]. | to isolate hantavirus from lishui county--one of the epidemic regions for hemorrhagic fever with renal syndrome (hfrs), in zhejiang province, and to identify the serotype and molecular/biological characteristics of a new htn subtype hantavirus (hv) strains, hopefully to provide evidence for hfrs prevention and therapy. | 2009 | 19565882 |
| efficacy of arbidol on lethal hantaan virus infections in suckling mice and in vitro. | arbidol is an immunomodulator that was first developed in russia. in this study, we report the antiviral activity of arbidol against hantaan virus (htnv) in vitro and in vivo. | 2009 | 19575005 |
| investigating the effects of climatic variables and reservoir on the incidence of hemorrhagic fever with renal syndrome in huludao city, china: a 17-year data analysis based on structure equation model. | hfrs is a serious public health problem in china and the study on hfrs is important in china for its large population. the present study aimed to explore the impact of climatic variables and reservoir on the incidence of hfrs in huludao city, an epidemic focus of the disease in northeastern china. | 2009 | 19583875 |
| analysis of the immune response to hantaan virus nucleocapsid protein c-terminal-specific cd8(+) t cells in patients with hemorrhagic fever with renal syndrome. | hantaan virus (htnv), the prototype member of the hantavirus genus in the family bunyaviridae, causes hemorrhagic fever with renal syndrome (hfrs), which is characterized by capillary leakage, hemorrhage, and renal injury, and is an important public health problem in china. some kinds of immune cells, particularly cd8(+) t cells, are involved in the pathogenesis of hantavirus infection. the nucleocapsid protein (np) of the hantavirus is the most conserved structural protein and the most abundant ... | 2009 | 19594396 |
| cellular immune response to hantaan virus nucleocapsid protein in the acute phase of hemorrhagic fever with renal syndrome: correlation with disease severity. | the cellular immune response to hantaan virus (htnv) is incompletely understood, especially in humans. | 2009 | 19072554 |
| hantaan virus nucleocapsid protein binds to importin alpha proteins and inhibits tumor necrosis factor alpha-induced activation of nuclear factor kappa b. | hantaviruses such as hantaan virus (htnv) and andes virus cause two human diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, respectively. for both, disease pathogenesis is thought to be immunologically mediated and there have been numerous reports of patients with elevated levels of proinflammatory and inflammatory cytokines, including tumor necrosis factor alpha (tnf-alpha), in their sera. multiple viruses have developed evasion strategies to circumvent the host ... | 2009 | 19019947 |
| experience with intravenous ribavirin in the treatment of hemorrhagic fever with renal syndrome in korea. | results of a clinical study using intravenous (iv) ribavirin for treating department of defense personnel with hemorrhagic fever with renal syndrome (hfrs) acquired in korea from 1987 to 2005 were reviewed to determine the clinical course of hfrs treated with iv ribavirin. a total of 38 individuals enrolled in the study had subsequent serological confirmation of hfrs. four of the 38 individuals received three or fewer doses of ribavirin and were excluded from treatment analysis. of the remaining ... | 2009 | 18977392 |
| construction and expression of a eukaryotic expression vector containing a fusion gene of the hantaan virus s gene and hsp70 gene. | hantavirus (hv) infection leads to a kind of severe systematic syndrome, hemorrhagic fever with renal syndrome (hfrs). heat shock proteins (hsps) can be used as adjuvants assisting soluble antigens to produce specific targets which can be attacked by cytotoxic t lymphocytes. for further research on hfrs vaccine, this study aimed to express hantaan virus nucleocapsid protein (htnv np)-hsp70 fusion protein in cos-7 cells. first, an htnv s gene encoding np was amplified by pcr with a mutated termin ... | 2009 | 18810536 |
| increased serum fibronectin levels in patients with hemorrhagic fever with renal syndrome. | in view of the role of fibronectin in adhesion, signal transduction pathways and the infectious disease process, changes in serum fibronectin levels may influence disease evolution and severity in patients with hemorrhagic fever with renal syndrome (hfrs). | 2010 | 19701604 |
| in vivo kinetics and biodistribution of a hantaan virus dna vaccine after intramuscular injection in mice. | to study the kinetics in vivo of a hantaan virus dna vaccine, we constructed a fusion dna vaccine, pegfp/s, by cloning the s segment of hantavirus into the vector, pegfp-c1, which encodes green fluorescent protein egfp. in this report, we provide evidence that pegfp/s was distributed and persistently expressed for more than 60 days in several organs after inoculation. our findings suggest that the persistent immune responses induced by a hantaan virus dna vaccine are likely due to the plasmid pe ... | 2010 | 20960291 |
| [construction and immunogenic study of recombinant adenovirus containing chimeric gene g2s0.7 and ctl epitopes of hantaan virus]. | to express g2 fragment of m segment and 0.7 kb fragment of s segment and several ctl epitopes of s segment in adenovirus expression system and investigate the immunological properties of hantaan virus chimeric gene. | 2010 | 21055343 |
| structural studies of hantaan virus. | hantaan virus is the prototypic member of the hantavirus genus within the family bunyaviridae and is a causative agent of the potentially fatal hemorrhagic fever with renal syndrome. the bunyaviridae are a family of negative-sense rna viruses with three-part segmented genomes. virions are enveloped and decorated with spikes derived from a pair of glycoproteins (gn and gc). here, we present cryo-electron tomography and single-particle cryo-electron microscopy studies of hantaan virus virions. we ... | 2010 | 21068243 |
| tracking hantavirus nucleocapsid protein using intracellular antibodies. | hantavirus nucleocapsid (n) protein is a multifunctional viral macromolecule involved in multiple stages of the viral replication cycle. the intracellular trafficking of n protein during virus assembly remains unclear. | 2010 | 21092325 |
| spatiotemporal trends and climatic factors of hemorrhagic fever with renal syndrome epidemic in shandong province, china. | hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne disease caused by hantaviruses. it is endemic in all 31 provinces, autonomous regions, and metropolitan areas in mainland china where human cases account for 90% of the total global cases. shandong province is among the most serious endemic areas. hfrs cases in shandong province were first reported in yutai county in 1968. since then, the disease has spread across the province, and as of 2005, all 111 counties were reported to have l ... | 2010 | 20706629 |