| molecular epidemiological analyses of cryptosporidium parvum virus 1 (cspv1), a symbiotic virus of cryptosporidium parvum, in japan. | we show that cryptosporidium parvum virus 1 (cspv1), a member of the family partitiviridae, genus cryspovirus that can infect cryptosporidium parvum, is a new candidate for high-resolution tool for tracing c. parvum. cspv1 was detected in all c. parvum-positive samples tested. phylogenetic analysis of dsrna1 sequence from cspv1 can distinguish infected areas of c. parvum on the national level. sequences detected in samples from iwate prefecture and other islands (tanegashima, and okinawa) belong ... | 2016 | 26439535 |
| molecular characterization of cryptosporidium parvum detected in japanese black and holstein calves in iwate prefecture and tanegashima island, kagoshima prefecture, japan. | cryptosporidium oocysts were found in 43 out of 77 calves from two farms in iwate prefecture and nine farms on tanegashima island, kagoshima prefecture, japan. the dna fragments of 18s ribosomal rna (18s rrna) gene were amplified by a nested pcr from 43 oocyst-positive as well as one oocyst-negative samples. all of them were precisely identified as c. parvum by analyzing the nucleotide sequences of the 18s rrna gene. c. parvum oocyst-positive calves ranged in age from 6 to 13 days old and signif ... | 2015 | 25819544 |