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screening breeding sites of the common toad (bufo bufo) in england and wales for evidence of endocrine disrupting activity.anuran amphibians are often present in agricultural landscapes and may therefore be exposed to chemicals in surface waters used for breeding. we used passive accumulation devices (spmd and pocis) to sample contaminants from nine breeding sites of the common toad (bufo bufo) across england and wales, measuring endocrine activity of the extracts in a recombinant yeast androgen screen (yas) and yeast estrogen screen (yes) and an in vitro vitellogenin induction screen in primary culture of xenopus l ...201525817886
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