Publications

reaction of the developing chicken embryo and the chorioallantoic membrane to inoculation with various strains of mycobacterium paratuberculosis (johne's bacillus).		1946	20275302
experimental mycobacterium paratuberculosis infection in chickens.		1972	5022402
isolation and identification of mycobacteria from porcine tissues: a three-year summary.	mycobacteria were isolated from 1,591 (78%) of 2,036 porcine tissues submitted to veterinary services laboratories over a 3-year period (july 1, 1971, to june 30, 1974). the isolates were identified by biochemical and serologic tests. of the 1,547 mycobacterium avium isolates, 452 were serotype 1, 728 were serotype 2, 60 were serotyped 4, 110 were serotype 8, and 51 were serotyped 10; 36 isolates represented 11 other serotypes; 65 isolates shared antigens with more than one serotype; and 45 isol ...	1975	1099946
possible role of mycobacteria in inflammatory bowel disease. i. an unclassified mycobacterium species isolated from patients with crohn's disease.	a previously unrecognized mycobacterium species was isolated from two patients with crohn's disease. the organism is an acid-fast, mycobactin-dependent mycobacterium that has characteristics which do not conform to any of the presently recognized species. it belongs to the runyon group iii mycobacteria and is most closely related to mycobacterium paratuberculosis. animal inoculation revealed pathogenicity for mice when injected intravenously or intraperitoneally, but not for rats, guinea pigs, r ...	1984	6499624
comparison of the pathogenicity of mycobacterium paratuberculosis and mycobacterium spp isolated from the wood pigeon (columba palumbus-l).	the pathogenicity of 4 strains of mycobacteria isolated from wood pigeons and 2 strains of mycobacterium paratuberculosis was compared in calves, mice and chickens. three of the 4 wood pigeon strains and the 2 m. paratuberculosis strains produced clinical johne's disease in calves. all 6 strains were pathogenic for mice and the 4 wood pigeon strains were pathogenic for chickens. the strains were not agglutinated by antisera to strains of the m. avium complex and all were mycobactin-dependent. it ...	1985	4067024
experimental disease in young chickens induced by a mycobacterium paratuberculosis isolate from a patient with crohn's disease.	the susceptibility of young chickens to infection with an isolate of mycobacterium paratuberculosis which had been recovered from diseased tissues of a patient with crohn's disease was determined. two-week-old leghorn-cochin chicks were inoculated with strain linda. six birds received 10(7) organisms orally, six intraperitoneally, and five intracardially. six uninoculated birds served as contact controls. birds from each group were necropsied at two-week intervals. focal granulomatous lesions oc ...	1991	1832080
immortalization and characterization of bovine peritoneal macrophages transfected with sv40 plasmid dna.	a transformed bovine peritoneal macrophage cell line was developed and characterized. primary peritoneal macrophages were transformed by calcium-phosphate transfection with sv40 plasmid dna. the transformed cell line retained the morphology of resident peritoneal macrophages as determined by light microscopy and histochemical analysis showed non-specific esterase activity. in addition, immunohistochemical staining of transformed peritoneal macrophages for lysozyme activity was positive. transfor ...	1995	7676607
comparative evaluation of pcr assays for the robust molecular detection of mycobacterium avium subsp. paratuberculosis.	mycobacterium avium subsp. paratuberculosis (map) can cause a very serious, often-fatal disease, namely paratuberculosis, in several animal species, especially ruminants. recently, it has also been implicated in the pathogenesis of infectious bowel disease of man. the aim of this study was to develop a molecular method for the routine detection and identification of map, from tissue samples of animal origin. the proposed assay would have to combine optimum performance and cost, with high reprodu ...	2004	14967222
immunological response to mycobacterium avium subsp. paratuberculosis in chickens.	enzyme-linked immunosorbent assay (elisa) and culture are 2 common diagnostic tests for detecting mycobacterium avium subsp. paratuberculosis (map) in johne's disease, but they are not as sensitive as polymerase chain reaction (pcr). however, inhibitors can coextract with the target dna and cause interference in pcr. development of an immune capture assay followed by pcr amplification can alleviate this problem. in this study, we were able to induce an immune response in chickens using heat or f ...	2004	15581226
rapid mycobacterial liquid culture-screening method for mycobacterium avium complex based on secreted antigen-capture enzyme-linked immunosorbent assay.	sensors in automated liquid culture systems for mycobacteria, such as mgit, bact/alert 3d, and trek esp ii, flag growth of any type of bacteria; a positive signal does not mean that the target mycobacteria are present. all signal-positive cultures thus require additional and often laborious testing. an immunoassay was developed to screen liquid mycobacterial cultures for evidence of mycobacterium avium complex (mac). the method, called the mac-enzyme-linked immunosorbent assay (elisa), relies on ...	2009	19261776
production of and applications for a polyclonal igy diagnostic reagent specific for mycobacterium avium subsp. paratuberculosis.	antibodies specific to the cell surface antigens of mycobacterium avium subsp. paratuberculosis (map) have multiple useful applications, e.g. organism detection, immunoconcentration, and cell visualization. the aim of this study was to produce and compare polyclonal antibodies for such research and diagnostic purposes. three polyclonal antibodies to map were produced using sera from immunized rabbits and chickens plus naturally infected cows. cross-reactive antibodies in each map antibody prepar ...	2009	19851733
development of an immunocapture-polymerase chain reaction assay using igy to detect mycobacterium avium subsp. paratuberculosis.	a diagnostic assay using immunomagnetic separation was developed to capture mycobacterium avium subsp. paratuberculosis (map) from bovine feces by means of igy derived from chicken eggs. the antibody was coupled directly onto the surface of magacell cellulose/iron oxide beads or indirectly by being mixed with magabeads and a rabbit igg linker against chicken antigen. optimization parameters for the immunocapture included incubation time, temperature, volume, and type of immunocapture beads. anal ...	2010	20592839
development of dna-designed avian igy antibodies for detection of mycobacterium avium subsp. paratuberculosis heat shock protein 70 (hsp70) and anti-hsp70 antibodies in the serum of normal cattle.	mycobacterium avium subsp. paratuberculosis heat shock protein 70 (maphsp70) is an immunodominant antigen, which can be used as a subunit vaccine against bovine paratuberculosis. in the present study, we evaluated the immunogenic activities of maphsp70 expressed by dna vaccine in chicken and the use of prepared specific avian igy antibodies for western blotting and elisa methods. the gene encoding map hsp70 was subcloned into the eukaryotic expression vector, pcdna3.1, and the recombinant plasmi ...	2012	22415790