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[isolation and identification of the akabane virus from mosquitoes in yunnan province, china].to evaluate the prevalence of mosquito-borne viruses in manshi and ruili (yunnan province, china), we collected 2 149 mosquitoes (17 species) in august 2010. virus isolation was undertaken by the cul- ture of baby hamster kidney cells (bhk-21 cells). two virus-like isolates were obtained: dhl10m117 was isolated from collected in mangshi; dhl10m110 was obtained from anopheles vagus collected in rui- li. both isolates caused cytopathic effects,illness and death in suckling mice inoculated with the ...201525997331
[characterization and analyses of the full-length genome of a strain of the akabane virus isolated from mosquitoes in yunnan province, china].we wished to sequence the full-length genomes of the dhl10m110 strain of the akabane virus (akv) isolated from mosquitoes in yunnan province, china, in 2010. we also wished to analyze the characteristics of these complete nucleotide sequences. the complete genomic sequence of the dhl10m110 strain from yunnan province was obtained by reverse transcription-polymerase chain reaction and direct sequencing. we found that the length of the l, m and s gene nucleotide sequences of the dhl10m110 strain w ...201627396159
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