Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| highly efficient site-specific mutagenesis in malaria mosquitoes using crispr. | anopheles mosquitoes transmit at least 200 million annual malaria infections worldwide. despite considerable genomic resources, mechanistic understanding of biological processes in anopheles has been hampered by a lack of tools for reverse genetics. here, we report successful application of the crispr/cas9 system for highly efficient, site-specific mutagenesis in the diverse malaria vectors anopheles albimanus, anopheles coluzzii, and anopheles funestus when guide rnas and cas9 protein are injec ... | 2017 | 29233915 |
| first detection of n1575y mutation in pyrethroid resistant anopheles gambiae in southern côte d'ivoire. | background. the intensification of insecticide use for both public health and agriculture in africa has contributed to growing insecticide resistance. today, resistance to world health organization (who)-approved insecticide classes is widespread. in an agricultural area of southern côte d'ivoire, the main malaria vector anopheles coluzzii shows multiple resistance across insecticides mediated by both target site mutation and metabolic mechanisms. to plan new vector control strategies and avert ... | 2017 | 29018842 |
| photoperiodic responses of sahelian malaria mosquitoes anopheles coluzzii and an. arabiensis. | throughout large parts of sub-saharan africa, seasonal malaria transmission follows mosquito density, approaching zero during the dry season and peaking during the wet season. the mechanisms by which malaria mosquitoes survive the long dry season, when no larval sites are available remain largely unknown, despite being long recognized as a critical target for vector control. previous work in the west african sahel has led to the hypothesis that anopheles coluzzii (formerly m-form anopheles gambi ... | 2017 | 29282150 |
| experimental evolution to increase the efficacy of the entomopathogenic fungus beauveria bassiana against malaria mosquitoes: effects on mycelial growth and virulence. | entomopathogenic fungi such as beauveria bassiana are currently considered as a potential control agent for malaria mosquitoes. the success of such strategies depends among others on the efficacy of the fungus to kill its hosts. as b. bassiana can use various resources for growth and reproduction, increasing the dependency on mosquitoes as a nutritional source may be instrumental for reaching this goal. passage of entomopathogenic fungi through an insect host has been shown to increase its virul ... | 2017 | 28515777 |
| [the anopheles gambiae species complex and kdr resistance gene at the periphery of douala, cameroon]. | this study was conducted from may to june 2015 in yassa (industrialized area) and logbessou (non-industrialized area), two peri-urban areas of the city of douala, cameroon with the aim of an assessment of the spatial distribution of the gambiae complex, the determination of their resistance to insecticides and the distribution of the kdr mutation. mosquito larvae were collected by the dipping method and nursed to adult stage. the sensitivity of adult anopheles gambiae s.l. populations to ddt and ... | 2017 | 28303549 |
| pre-copula acoustic behaviour of males in the malarial mosquitoes anopheles coluzzii and anopheles gambiae s.s. does not contribute to reproductive isolation. | we reveal that males of two members of the anopheles gambiae s.l. species complex, anopheles coluzzii and anopheles gambiae s.s. (hereafter a. gambiae), which are both malaria vectors, perform a stereotypical acoustic behaviour in response to pure tones at frequencies that encompass the frequency range of the female's flight-tones. this behaviour resembles that described for culex quinquefasciatus and consists of phonotactic flight initiated by a steep increase in wing-beat frequency (wbf) follo ... | 2017 | 28148817 |
| [distribution of species and kdr gene frequency among anopheles gambiae s.s. and anopheles coluzzii populations in five agricultural sites in côte d'ivoire]. | the resistance of anopheles gambiae s.l. to insecticides constitutes a concern for the programs of malaria control because it can be an obstacle to effective control of the vectors. the follow-up of this resistance is a priority to work out strategies of management and to preserve the means of that major malaria vector control activities. the general objective of this study is to identify the species within an. gambiae s.l., and to determine the frequency of the kdr gene in an. gambiae s.s. and ... | 2017 | 28357641 |
| the contribution of dietary restriction to extended longevity in the malaria vector anopheles coluzzii. | variation in longevity has long been of interest in vector biology because of its implication in disease transmission through vectorial capacity. recent studies suggest that anopheles coluzzii adults persist during the ~7 month dry season via aestivation. recently there has been a growing body of evidence linking dietary restriction and low ratio of dietary protein to carbohydrate with extended longevity of animals. here, we evaluated the effects of dietary restriction and the protein : carbohyd ... | 2017 | 28340627 |
| the use of sequential mark-release-recapture experiments to estimate population size, survival and dispersal of male mosquitoes of the anopheles gambiae complex in bana, a west african humid savannah village. | vector control is a major component of the malaria control strategy. the increasing spread of insecticide resistance has encouraged the development of new tools such as genetic control which use releases of modified male mosquitoes. the use of male mosquitoes as part of a control strategy requires an improved understanding of male mosquito biology, including the factors influencing their survival and dispersal, as well as the ability to accurately estimate the size of a target mosquito populatio ... | 2017 | 28784147 |
| microbiota-induced peritrophic matrix regulates midgut homeostasis and prevents systemic infection of malaria vector mosquitoes. | manipulation of the mosquito gut microbiota can lay the foundations for novel methods for disease transmission control. mosquito blood feeding triggers a significant, transient increase of the gut microbiota, but little is known about the mechanisms by which the mosquito controls this bacterial growth whilst limiting inflammation of the gut epithelium. here, we investigate the gut epithelial response to the changing microbiota load upon blood feeding in the malaria vector anopheles coluzzii. we ... | 2017 | 28545061 |
| presence of susceptible wild strains of anopheles gambiae in a large industrial palm farm located in aboisso, south-eastern of côte d'ivoire. | the effectiveness of malaria control programmes through implementation of vector control activities is challenged by the emergence of insecticide resistance. in the south-eastern region of côte d'ivoire, where palm oil plantations remain the predominant agricultural crop, the susceptibility of wild anopheles gambiae sensu lato species is still unknown and thus requires a particular attention. the current study was carried out to address the gap by in-depth characterization of susceptibility leve ... | 2017 | 28427450 |
| massive introgression drives species radiation at the range limit of anopheles gambiae. | impacts of introgressive hybridisation may range from genomic erosion and species collapse to rapid adaptation and speciation but opportunities to study these dynamics are rare. we investigated the extent, causes and consequences of a hybrid zone between anopheles coluzzii and anopheles gambiae in guinea-bissau, where high hybridisation rates appear to be stable at least since the 1990s. anopheles gambiae was genetically partitioned into inland and coastal subpopulations, separated by a central ... | 2017 | 28417969 |
| tracing the origin of the early wet-season anopheles coluzzii in the sahel. | in arid environments, the source of the malaria mosquito populations that re-establish soon after first rains remains a puzzle and alternative explanations have been proposed. using genetic data, we evaluated whether the early rainy season (rs) population of anopheles coluzzii is descended from the preceding late rs generation at the same locality, consistent with dry season (ds) dormancy (aestivation), or from migrants from distant locations. distinct predictions derived from these two hypothes ... | 2017 | 28717390 |
| the invasive shrub prosopis juliflora enhances the malaria parasite transmission capacity of anopheles mosquitoes: a habitat manipulation experiment. | a neglected aspect of alien invasive plant species is their influence on mosquito vector ecology and malaria transmission. invasive plants that are highly attractive to anopheles mosquitoes provide them with sugar that is critical to their survival. the effect on anopheles mosquito populations was examined through a habitat manipulation experiment that removed the flowering branches of highly attractive prosopis juliflora from selected villages in mali, west africa. | 2017 | 28676093 |
| chemosensory gene expression in olfactory organs of the anthropophilic anopheles coluzzii and zoophilic anopheles quadriannulatus. | anopheles (an.) coluzzii, one of africa's primary malaria vectors, is highly anthropophilic. this human host preference contributes greatly to its ability to transmit malaria. in contrast, the closely related an. quadriannulatus prefers to feed on bovids and is not thought to contribute to malaria transmission. the diverged preference for host odor profiles between these sibling species is likely reflected in chemosensory gene expression levels in the olfactory organs. therefore, we compared the ... | 2017 | 28938869 |
| relating high insecticide residues in larval breeding habitats in urban residential areas to the selection of pyrethroid resistance in anopheles gambiae s.l. (diptera: culicidae) in akim oda, ghana. | the main objective of this study was to assess insecticide contamination in anopheles breeding habitats in urban residential areas and pyrethroid susceptibility status of mosquitoes found in the habitats. a larval survey was conducted in akim oda between july and october 2016. the larvae that were reared to adult were used for susceptibility test against four different pyrethroid insecticides (deltamethrin 0.05%, permethrin 0.75%, cyfluthrin 0.15%, and etofenprox 0.5%). gas chromatography was us ... | 2017 | 29267961 |
| using maldi-tof ms to identify mosquitoes collected in mali and their blood meals. | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. to test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of mali. we successfully analysed the blood meals from 651 mosquito abdomens crushed on whatman filter paper (wfps) in the field using maldi-t ... | 2018 | 29409547 |
| distinct physiological, biochemical and morphometric adjustments in the malaria vectors anopheles gambiae and an. coluzzii as means to survive to dry season conditions in burkina faso. | aestivation and dispersive migration are the two strategies evoked in literature to explain the way by which malaria vectors, anopheles coluzzii and an. gambiae, survive the harsh climatic conditions of the dry season in sub-saharan africa. however, the physiological mechanisms regulating these two strategies are unknown. here, mosquito species were submitted to controlled environmental parameters mimicking the rainy and dry seasons conditions of south-western burkina faso. survival strategies w ... | 2018 | 29378815 |
| native entomopathogenic metarhizium spp. from burkina faso and their virulence against the malaria vector anopheles coluzzii and non-target insects. | genetically enhanced metarhizium pingshaense are being developed for malaria vector control in burkina faso. however, not much is known about the local prevalence and pathogenicity of this fungus, so we prospected mosquitoes and plant roots (a common habitat for metarhizium spp.) for entomopathogenic fungi. | 2018 | 29587876 |
| functional characterization of the dual allatostatin-a receptors in mosquitoes. | the neuropeptide allatostatin-a (asta) and its cognate receptors (astars) are involved in the modulation of feeding behavior, which in hematophagous insects includes the regulation of the disease vector-related behaviors, host seeking and blood feeding. in mosquitoes and other dipterans, there are two copies of astar, contrasting with the single copy found in other insects. in this study, we identified and cloned the dual astar system of two important disease vectors aedes aegypti and culex quin ... | 2018 | 29103918 |
| the fate of genes that cross species boundaries after a major hybridization event in a natural mosquito population. | animal species are able to acquire new genetic material via hybridization and subsequent introgression. however, little is known about how foreign genomic material is incorporated into a population over time and what genes are susceptible to introgression. here, we follow the closely related mosquito sister species anopheles coluzzii and anopheles gambiae in a sympatric natural population in mali at multiple time points spanning a period of 25 years. during this period, we observed the temporary ... | 2018 | 30447117 |
| exploring insecticide resistance mechanisms in three major malaria vectors from bangui in central african republic. | malaria remains the main cause of mortality and morbidity in the central african republic. however, the main malaria vectors remain poorly characterised, preventing the design of suitable control strategies. here, we characterised the patterns and mechanisms of insecticide resistance in three important vectors from bangui. mosquitoes were collected indoors, using electrical aspirators in july 2016 in two neighborhoods at bangui. who bioassays performed, using f2 an. gambiae sensu lato (s.l.), re ... | 2018 | 30433868 |
| adjusted likelihood-ratio test for variants with unknown genotypes. | association tests performed with the likelihood-ratio test (lr test) can be an alternative to [formula: see text], which is often used in population genetics to find variants of interest. because the lr test has several properties that could make it preferable to [formula: see text], we propose a novel approach for modeling unknown genotypes in highly-similar species. to show the effectiveness of this lr test approach, we apply it to single-nucleotide polymorphisms (snps) associated with the rec ... | 2018 | 30419783 |
| modelling the persistence of mosquito vectors of malaria in burkina faso. | populations of the anopheles gambiae complex are found during the rainy season throughout west africa, even in arid areas with long dry seasons during which mosquitoes appear to be absent. several hypotheses have been proposed to explain this apparent paradox, including aestivation, dispersal between neighbouring settlements, and long distance migration using high-altitude wind currents. | 2018 | 29609598 |
| targeted application of an organophosphate-based paint applied on windows and doors against anopheles coluzzii resistant to pyrethroids under real life conditions in vallée du kou, burkina faso (west africa). | a novel strategy applying an organophosphate-based insecticide paint on doors and windows in combination with long-lasting insecticide-treated nets (llins) was tested for the control of pyrethroid-resistant malaria vectors in a village setting in vallée du kou, a rice-growing area west of burkina faso. | 2018 | 29609597 |
| functional characterization of mosquito short neuropeptide f receptors. | mosquito blood feeding transiently inhibits sugar- and host seeking through neuropeptide signaling. short neuropeptide f (snpf) is one of the neuromodulators involved in this regulation. here, we identified the genes for the snpf precursor and the snpf receptor in the southern house mosquito, culex quinquefasciatus. comparative analyses are made with the genes of the snpf precursor and receptor from two other important vectors, aedes aegypti and anopheles coluzzii. we functionally characterized ... | 2018 | 29550617 |
| multiband modulation spectroscopy for the determination of sex and species of mosquitoes in flight. | we present a dual-wavelength polarimetric measurement method to distinguish species and sexes of disease transmitting mosquitoes in flight. by measuring co- and de-polarized backscattered light at 808 and 1550 nm, the degree of linear polarization, wingbeat frequency, reflectance, spectral ratio and glossiness of mosquitoes can be retrieved. body and wing contributions to these signals can be separated. whereas the optical cross section is sensitive to the aspect of observation, thus the heading ... | 2018 | 29508537 |
| entomological impact of indoor residual spraying with pirimiphos-methyl: a pilot study in an area of low malaria transmission in senegal. | scaling-up of effective anti-malarial control strategies in central-west region of senegal has resulted in the sharp decline in malaria prevalence in this area. however, despite these strategies, residual malaria transmission has been observed in some villages (hot spots). the objective of this study was to assess the impact of indoor residual spraying (irs) with pirimiphos-methyl on malaria transmission in hot spot areas. | 2018 | 29402274 |
| using the human blood index to investigate host biting plasticity: a systematic review and meta-regression of the three major african malaria vectors. | the proportion of mosquito blood-meals that are of human origin, referred to as the 'human blood index' or hbi, is a key determinant of malaria transmission. | 2018 | 30563533 |
| analysis of natural female post-mating responses of anopheles gambiae and anopheles coluzzii unravels similarities and differences in their reproductive ecology. | anopheles gambiae and an. coluzzii, the two most important malaria vectors in sub-saharan africa, are recently radiated sibling species that are reproductively isolated even in areas of sympatry. in females from these species, sexual transfer of male accessory gland products, including the steroid hormone 20-hydroxyecdysone (20e), induces vast behavioral, physiological, and transcriptional changes that profoundly shape their post-mating ecology, and that may have contributed to the insurgence of ... | 2018 | 29700344 |
| rapid selection of a pyrethroid metabolic enzyme cyp9k1 by operational malaria control activities. | since 2004, indoor residual spraying (irs) and long-lasting insecticide-impregnated bednets (llins) have reduced the malaria parasite prevalence in children on bioko island, equatorial guinea, from 45% to 12%. after target site-based (knockdown resistance; kdr) pyrethroid resistance was detected in 2004 in anopheles coluzzii (formerly known as the m form of the anopheles gambiae complex), the carbamate bendiocarb was introduced. subsequent analysis showed that kdr alone was not operationally sig ... | 2018 | 29674455 |
| characterization of chemosensory responses on the labellum of the malaria vector mosquito, anopheles coluzzii. | anopheles gambiae coluzzii (an. coluzzii) uses olfaction to modulate a range of critical behaviors that are essential for survival and reproduction; most notably, host preference and selection underlie its vectorial capacity for human malaria. as is the case for all mosquitoes, an. coluzzii has three specialized peripheral olfactory appendages-the antennae, maxillary palps and labella-which are used to detect and orient in response to a large variety of olfactory cues. of these, neither the mole ... | 2018 | 29618749 |
| genetic variation associated with increased insecticide resistance in the malaria mosquito, anopheles coluzzii. | malaria mortality rates in sub-saharan africa have declined significantly in recent years as a result of increased insecticide-treated bed net (itn) usage. a major challenge to further progress is the emergence and spread of insecticide resistance alleles in the anopheles mosquito vectors, like an. coluzzii. a non-synonymous mutation in the para voltage-gated sodium channel gene reduces pyrethroid-binding affinity, resulting in knockdown resistance (kdr). metabolic mechanisms of insecticide resi ... | 2018 | 29618373 |
| investigation of the seasonal microbiome of anopheles coluzzii mosquitoes in mali. | the poorly understood mechanisms of dry season persistence of anopheles spp. mosquitoes through the dry season in africa remain a critical gap in our knowledge of plasmodium disease transmission. while it is thought that adult mosquitoes remain in a dormant state throughout this seven-month dry season, the nature of this state remains unknown and has largely not been recapitulated in laboratory settings. to elucidate possible connections of this state with microbial composition, the whole body m ... | 2018 | 29596468 |
| investigating insecticide resistance and knock-down resistance (kdr) mutation in dielmo, senegal, an area under long lasting insecticidal-treated nets universal coverage for 10 years. | the use of insecticides, through indoor residual spraying and long-lasting insecticide-treated nets (llins), is essential to control malaria vectors. however, the sustainability of these tools is challenged by the spread of insecticide resistance in anopheles mosquitoes. this study was conducted to assess the susceptibility to insecticides and to determine the resistance mechanisms in malaria vectors in dielmo, a rural area of western senegal where llins were introduced a decade ago. | 2018 | 29566682 |
| insecticidal activity of local plants essential oils against laboratory and field strains of anopheles gambiae s. l. (diptera: culicidae) from burkina faso. | the emergence and intensification of resistance to insecticides in malaria vector populations is the main obstacle to insecticide-based control efforts. the main objective of this study was to evaluate the larvicidal and adulticidal properties of the essential oils (eos) of ocimum canum, hyptis suaveolens, hyptis spicigera, and lantana camara on field-collected, pyrethroids-resistant mosquitoes, local laboratory strains, and susceptible 'kisumu' strain of anopheles gambiae (meigen) (diptera: cul ... | 2018 | 30281085 |
| flight behaviour of malaria mosquitoes around odour-baited traps: capture and escape dynamics. | host-seeking mosquitoes rely on a range of sensory cues to find and approach blood hosts, as well as to avoid host detection. by using odour blends and visual cues that attract anthropophilic mosquitoes, odour-baited traps have been developed to monitor and control human pathogen-transmitting vectors. although long-range attraction of such traps has already been studied thoroughly, close-range response of mosquitoes to these traps has been largely ignored. here, we studied the flight behaviour o ... | 2018 | 30225014 |
| blood meal identification in the cryptic species anopheles gambiae and anopheles coluzzii using maldi-tof ms. | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has recently emerged in entomology as a technique to identify arthropods and their blood meal source. in this study, female anopheles gambiae were fed on five host blood sources: ocelot (leopardus pardalis), binturong (arctictis binturong), springbok (antidorcas marsupialis), jaguar (panthera onca) and hamadryas baboon (papio hamadryas), while anopheles coluzzii were fed on three hosts: dromedary (camelus ... | 2018 | 30052501 |
| highly focused transcriptional response of anopheles coluzzii to o'nyong nyong arbovirus during the primary midgut infection. | anopheles mosquitoes are efficient vectors of human malaria, but it is unknown why they do not transmit viruses as well as aedes and culex mosquitoes. the only arbovirus known to be consistently transmitted by anopheles mosquitoes is o'nyong nyong virus (onnv, genus alphavirus, family togaviridae). the interaction of anopheles mosquitoes with rna viruses has been relatively unexamined. | 2018 | 29986645 |
| predicting the likelihood and intensity of mosquito infection from sex specific plasmodium falciparum gametocyte density. | understanding the importance of gametocyte density on human-to-mosquito transmission is of immediate relevance to malaria control. previous work (churcher et al., 2013) indicated a complex relationship between gametocyte density and mosquito infection. here we use data from 148 feeding experiments on naturally infected gametocyte carriers to show that the relationship is much simpler and depends on both female and male parasite density. the proportion of mosquitoes infected is primarily determin ... | 2018 | 29848446 |
| transgenic metarhizium pingshaense synergistically ameliorates pyrethroid-resistance in wild-caught, malaria-vector mosquitoes. | transgenic metarhizium pingshaense expressing the spider neurotoxin hybrid (met-hybrid) kill mosquitoes faster and at lower spore doses than wild-type strains. in this study, we demonstrate that this approach dovetails with the cornerstone of current malaria control: pyrethroid-insecticides, which are the cornerstone of current malaria control. we used world health organization (who) tubes, to compare the impact on insecticide resistance of met-hybrid with red fluorescent m. pingshaense (met-rfp ... | 2018 | 30192847 |
| unexpectedly high plasmodium sporozoite rate associated with low human blood index in anopheles coluzzii from a llin-protected village in burkina faso. | despite the effectiveness of mass distribution of long-lasting insecticidal nets (llins) in reducing malaria transmission in africa, in hyperendemic areas such as burkina faso the burden of malaria remains high. we here report the results of a 4-month survey on the feeding habits and plasmodium infection in malaria vectors from a village in burkina faso one year following a national llin distribution programme. low values of human blood index (hbi) observed in the major malaria vectors in the ar ... | 2018 | 30143698 |
| blood feeding behaviour comparison and contribution of anopheles coluzzii and anopheles gambiae, two sibling species living in sympatry, to malaria transmission in alibori and donga region, northern benin, west africa. | the main goal of this study was to assess the blood feeding behaviour and the contribution anopheles coluzzii and anopheles gambiae, 2 sibling species of an. gambiae sensu stricto. present and living in sympatry in 2 regions of northern benin targeted for indoor residual spraying (irs). | 2018 | 30134912 |
| insecticide resistance in anopheles gambiae from the northern democratic republic of congo, with extreme knockdown resistance (kdr) mutation frequencies revealed by a new diagnostic assay. | mutations in the voltage-gated sodium channel at codon 1014 confer knock-down resistance (kdr) to pyrethroids in a wide range of insects. anopheles gambiae exhibits two mutant alleles at codon 1014, serine and phenylalanine; and both are now widespread across africa. existing screening methods only allow for one resistant allele to be detected per assay. a new locked nucleic acid (lna) qpcr assay was developed for the simultaneous detection of both mutant alleles and the wild type allele in a si ... | 2018 | 30400885 |
| assessment of the developmental success of anopheles coluzzii larvae under different nutrient regimes: effects of diet quality, food amount and larval density. | in a context of increasing resistance of both vectors toward main classes of insecticides used in public health and parasites toward anti-malarial drugs, development of new and complementary molecules or control approaches is fundamental to achieve the objective of controlling or even eliminating malaria. concerning vector control, the sterile insect technique and other genetic control approaches are among promising complementary tools in an integrated management strategy for malaria control. th ... | 2018 | 30348155 |
| emergence of knock-down resistance in the anopheles gambiae complex in the upper river region, the gambia, and its relationship with malaria infection in children. | insecticide resistance threatens malaria control in sub-saharan africa. knockdown resistance to pyrethroids and organochlorines in anopheles gambiae sensu lato (s.l.) is commonly caused by mutations in the gene encoding a voltage-gated sodium channel which is the target site for the insecticide. the study aimed to examine risk factors for knockdown resistance in an. gambiae s.l. and its relationship with malaria infection in children in rural gambia. point mutations at the vgsc-1014 locus, were ... | 2018 | 29776361 |
| first report of the presence of l1014s knockdown-resistance mutation in anopheles gambiae s.s and anopheles coluzzii from togo, west africa. | background: to optimize the success of insecticide-based malaria control intervention, knowledge of the distribution of anopheles gambiae species and insecticide resistance mechanisms is necessary. this paper reported an updated data on pyrethroids/ddt resistance in the an. gambiae s.l population from togo. methods: from december 2013 to april 2015, females of indoor-resting an. gambiae s.l were captured in three locations belonging to three different ecological zones. resistance to ddt, permet ... | 2018 | 29707654 |
| a novel nested polymerase chain reaction assay targeting plasmodium mitochondrial dna in field-collected anopheles mosquitoes. | sensitive techniques for the detection of plasmodium (aconoidasida: plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. a real-time polymerase chain reaction (rt-pcr) protocol targeting plasmodium mtdna proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting plasmodium circumsporozoite protein (csp-elisa). however, because of the rel ... | 2018 | 29344968 |
| a high-quality de novo genome assembly from a single mosquito using pacbio sequencing. | a high-quality reference genome is a fundamental resource for functional genetics, comparative genomics, and population genomics, and is increasingly important for conservation biology. pacbio single molecule, real-time (smrt) sequencing generates long reads with uniform coverage and high consensus accuracy, making it a powerful technology for de novo genome assembly. improvements in throughput and concomitant reductions in cost have made pacbio an attractive core technology for many large genom ... | 2019 | 30669388 |
| high levels of knockdown resistance in anopheles coluzzii and anopheles gambiae (diptera: culicidae), major malaria vectors in togo, west africa: a 2011 monitoring report. | a survey of susceptibility to ddt, deltamethrin, bendiocarb, and chlorpyrifos-methyl was conducted in five localities in 2011 in togo, west africa, to assess the insecticide resistance status of anopheles gambiae s.l. (diptera: culicidae). female populations of an. gambaie s.l. emerged from collected larvae (f0) were exposed to insecticide-impregnated papers using world health organization test kits for adult mosquitoes; the susceptible reference strain kisumu was used as a control. resistance t ... | 2019 | 30924857 |
| investigating the blood-host plasticity and dispersal of anopheles coluzzii using a novel field-based methodology. | the biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. | 2019 | 30909960 |
| dual role of the anopheles coluzzii venus kinase receptor in both larval growth and immunity. | vector-borne diseases and especially malaria are responsible for more than half million deaths annually. the increase of insecticide resistance in wild populations of anopheles malaria vectors emphasises the need for novel vector control strategies as well as for identifying novel vector targets. venus kinase receptors (vkrs) constitute a receptor tyrosine kinase (rtk) family only found in invertebrates. in this study we functionally characterized anopheles vkr in the gambiae complex member, ano ... | 2019 | 30837655 |
| introgression between anopheles gambiae and anopheles coluzzii in burkina faso and its associations with kdr resistance and plasmodium infection. | insecticide resistance in anopheles coluzzii mosquitoes has become widespread throughout west africa including in burkina faso. the insecticide resistance allele (kdr or l1014f) is a prime indicator that is highly correlated with phenotypic resistance in west africa. studies from benin, ghana and mali have suggested that the source of the l1014f is introgression of the 2l divergence island via interspecific hybridization with anopheles gambiae. the goal of this study was to characterize local mo ... | 2019 | 30971230 |
| interaction of rna viruses of the natural virome with the african malaria vector, anopheles coluzzii. | mosquitoes are colonized by a little-studied natural virome. like the bacterial microbiome, the virome also probably influences the biology and immunity of mosquito vector populations, but tractable experimental models are lacking. we recently discovered two novel viruses in the virome of wild anopheles and in colonies of the malaria vector anopheles coluzzii: anopheles c virus and anopheles cypovirus. here, we describe biological interactions between these two viruses and an. coluzzii mosquitoe ... | 2019 | 31004099 |
| rapid high throughput sybr green assay for identifying the malaria vectors anopheles arabiensis, anopheles coluzzii and anopheles gambiae s.s. giles. | the anopheles gambiae sensu lato species complex consists of a number of cryptic species with different habitats and behaviours. these morphologically indistinct species are identified by chromosome banding. several molecular diagnostic techniques for distinguishing between an. coluzzii and an. gambiae are still under improvement. although, the current sine method for identification between an. coluzzii and an. gambiae works reliably, this study describes a refinement of the sine method to incre ... | 2019 | 31002694 |
| functional analysis of the three major pgrplc isoforms in the midgut of the malaria mosquito anopheles coluzzii. | peptidoglycan recognition proteins (pgrps) constitute the primary means of bacterial recognition in insects. recent work in the model organism drosophila has revealed the mechanisms by which the complement of pgrps refine the sensitivity of different tissues to bacterial elicitors, permitting the persistence of commensal bacteria in the gut whilst maintaining vigilance against bacterial infection. here, we use in vivo knockdowns and in vitro pull-down assays to investigate the role of the three ... | 2019 | 31760136 |
| the male mosquito contribution towards malaria transmission: mating influences the anopheles female midgut transcriptome and increases female susceptibility to human malaria parasites. | mating causes dramatic changes in female physiology, behaviour, and immunity in many insects, inducing oogenesis, oviposition, and refractoriness to further mating. females from the anopheles gambiae species complex typically mate only once in their lifetime during which they receive sperm and seminal fluid proteins as well as a mating plug that contains the steroid hormone 20-hydroxyecdysone. this hormone, which is also induced by blood-feeding, plays a major role in activating vitellogenesis f ... | 2019 | 31697788 |
| de novo profiling of rna viruses in anopheles malaria vector mosquitoes from forest ecological zones in senegal and cambodia. | mosquitoes are colonized by a large but mostly uncharacterized natural virome of rna viruses, and the composition and distribution of the natural rna virome may influence the biology and immunity of anopheles malaria vector populations. | 2019 | 31429704 |
| influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector anopheles coluzzii. | enhancers are cis-regulatory elements that control most of the developmental and spatial gene expression in eukaryotes. genetic variation of enhancer sequences is known to influence phenotypes, but the effect of enhancer variation upon enhancer functional activity and downstream phenotypes has barely been examined in any species. in the african malaria vector, anopheles coluzzii, we identified candidate enhancers in the proximity of genes relevant for immunity, insecticide resistance, and develo ... | 2019 | 31649293 |
| hold your breath - differential behavioral and sensory acuity of mosquitoes to acetone and carbon dioxide. | host seeking in the yellow fever mosquito, aedes aegypti, and the african malaria mosquito, anopheles coluzzii, relies on specific and generic host-derived odorants. previous analyses indicate that the behavioral response of these species depends differentially on the presence of carbon dioxide (co2) and other constituents in human breath for activation and attraction. in this study, we use a flight tube assay and electrophysiological analysis to assess the role of acetone, a major component of ... | 2019 | 31887129 |
| effects of indoor residual spraying and outdoor larval control on anopheles coluzzii from são tomé and príncipe, two islands with pre-eliminated malaria. | vector control is a key component of malaria prevention. two major vector control strategies have been implemented in são tomé and príncipe (stp), indoor residual spraying (irs) and outdoor larval control using bacillus thuringiensis israelensis (bti). this study evaluated post-intervention effects of control strategies on vector population density, composition, and knockdown resistance mutation, and their implications for malaria epidemiology in stp. | 2019 | 31806029 |
| pesticides and the evolution of the genetic structure of anopheles coluzzii populations in some localities in benin (west africa). | changes in the natural habitats of insect groups are determined the genetic polymorphisms between individuals. the objective of this study was to establish the genetic structure of the anopheles coluzzii populations in four localities of benin. | 2019 | 31805939 |
| high insecticide resistance in the major malaria vector anopheles coluzzii in chad republic. | the sahel region of chad republic is a prime candidate for malaria pre-elimination. to facilitate pre-elimination efforts in this region, two populations of anopheles coluzzii from central chad republic were characterized, their insecticide resistance profile and the possible molecular mechanisms driving the resistance in the field investigated. | 2019 | 31796068 |
| genetic structure of anopheles gambiae s.s populations following the use of insecticides on several consecutive years in southern benin. | several studies have reported the strong resistance of anopheles gambiae s.l. complex species to pyrethroids. the voltage-dependent sodium channel (vgsc) gene is the main target of pyrethroids and ddt. in benin, the frequency of the resistant allele (l1014f) of this gene varies along the north-south transect. monitoring the evolution of resistance is necessary to better appreciate the genetic structure of vector populations in localities subject to the intensive use of chemicals associated with ... | 2019 | 31007534 |
| commonly used insect repellents hide human odors from anopheles mosquitoes. | the mode of action for most mosquito repellents is unknown. this is primarily due to the difficulty in monitoring how the mosquito olfactory system responds to repellent odors. here, we used the q-system of binary expression to enable activity-dependent ca2+ imaging in olfactory neurons of the african malaria mosquito anopheles coluzzii. this system allows neuronal responses to common insect repellents to be directly visualized in living mosquitoes from all olfactory organs, including the antenn ... | 2019 | 31630950 |
| high plasmodium infection and multiple insecticide resistance in a major malaria vector anopheles coluzzii from sahel of niger republic. | information on insecticide resistance and the mechanisms driving it in the major malaria vectors is grossly lacking in niger republic, thus hindering control efforts. to facilitate evidence-based malaria control, the role of anopheles coluzzii population from southern niger, in malaria transmission, its insecticides resistance profile and the molecular mechanisms driving the resistance were characterized. | 2019 | 31126311 |
| the influence of larval stage and density on oviposition site-selection behavior of the afrotropical malaria mosquito anopheles coluzzii (diptera: culicidae). | in the selection of oviposition sites female mosquitoes use various cues to assess site quality to optimize survival of progeny. the presence of conspecific larvae influences this process. interactive effects of oviposition site selection were studied in the malaria mosquito anopheles coluzzii coetzee & wilkerson in dual- and no-choice assays, by exposing single gravid mosquitoes to oviposition cups containing 1) larvae of different developmental stages, 2) larvae-conditioned water (lcw), and 3) ... | 2019 | 31630193 |
| current versus future reproductive investment adaptive responses in adult anopheles coluzzii malaria mosquitoes: hydric-stressed males give it all. | life history theory predicts that an individual's current reproductive investment should depend on its future reproductive value. a variety of intrinsic biotic and extrinsic factors influence reproductive value, including age, health status and current environmental conditions. phenotypic plastic reproductive decisions are particularly crucial in species with limited mating and breeding opportunities. in the malaria mosquito anopheles coluzzii, the combination of male-male competition and female ... | 2019 | 31358037 |
| evidence of insecticide resistance selection in wild anopheles coluzzii mosquitoes due to agricultural pesticide use. | the wetlands used for some agricultural activities constitute productive breeding sites for many mosquito species. thus, the agricultural use of insecticide targeting other pests may select for insecticide resistance in malaria mosquitoes. the purpose of this study is to clarify some knowledge gaps on the role of agrochemicals in the development of insecticide resistance in malaria vectors is of utmost importance for vector control. | 2019 | 31307509 |
| age-dependent regulation of host seeking in anopheles coluzzii. | behavioural attraction of the malaria vector anopheles coluzzii to human host odour increases during adult maturation. we have previously demonstrated that the onset of host seeking in an. coluzzii coincides with an increased sensitivity of the co2-sensitive neurons and abundance of chemosensory receptor gene transcripts in the maxillary palp. in this study, we extend our analysis to the antenna. functional characterisation of the near-complete repertoire of odorant receptors (ors) expressed in ... | 2019 | 31273284 |
| spatial and temporal development of deltamethrin resistance in malaria vectors of the anopheles gambiae complex from north cameroon. | the effectiveness of insecticide-based malaria vector control interventions in africa is threatened by the spread and intensification of pyrethroid resistance in targeted mosquito populations. the present study aimed at investigating the temporal and spatial dynamics of deltamethrin resistance in an. gambiae s.l. populations from north cameroon. mosquito larvae were collected from 24 settings of the garoua, pitoa and mayo oulo health districts (hds) from 2011 to 2015. two to five days old female ... | 2019 | 30779799 |
| does artemether-lumefantrine administration affect mosquito olfactory behaviour and fitness? | artemisinin-based combination therapy (act) is the recommended treatment against uncomplicated plasmodium falciparum infections, and act is widely used. it has been shown that gametocytes may be present after act and transmission to mosquitoes is still possible. artemether-lumefantrine (al) is a broadly used artemisinin-based combination medicine. here, it is tested whether al influences behaviour and fitness of anopheles mosquitoes, which are the main vectors of p. falciparum. | 2019 | 30691446 |
| insecticide resistance profiles of anopheles gambiae s.l. in togo and genetic mechanisms involved, during 3-year survey: is there any need for resistance management? | malaria, one of the world's greatest public health challenges, is an endemic disease with stable transmission in togo. combating malaria requires an effective vector control. this study provides temporal data on insecticide resistance status in the major malaria vector anopheles gambiae sensu lato (s.l.) from togo. | 2019 | 31118032 |
| the current distribution and characterization of the l1014f resistance allele of the kdr gene in three malaria vectors (anopheles gambiae, anopheles coluzzii, anopheles arabiensis) in benin (west africa). | the fight against malaria faces various biological obstacles, including the resistance of parasites to anti-malarial drugs and the resistance of mosquito vectors to insecticides. the resistance of anopheles gambiae sensu lato (s.l.) to pyrethroids, the only class of insecticides used to impregnate mosquito nets, is known in benin; the expansion of this resistance is influenced by the existence of gene flow between species, otherwise by the presence or absence of the kdr mutation in them. the obj ... | 2019 | 31113418 |
| temporal escalation of pyrethroid resistance in the major malaria vector anopheles coluzzii from sahelo-sudanian region of northern nigeria. | despite the highest global burden of malaria, information on bionomics and insecticide resistance status of malaria vectors is grossly lacking in the densely populated sahelo-sudanian region of nigeria. to support evidence-based vector control we characterised transmission and resistance profiles of anopheles coluzzii populations from three sites in northern nigeria. high sporozoite infection (~19.51%) was found in the an. coluzzii populations. a high pyrethroid resistance was observed with only ... | 2019 | 31089196 |
| streamlined smfa and mosquito dark-feeding regime significantly improve malaria transmission-blocking assay robustness and sensitivity. | the development of malaria transmission-blocking strategies including the generation of malaria refractory mosquitoes to replace the wild populations through means of gene drives hold great promise. the standard membrane feeding assay (smfa) that involves mosquito feeding on parasitized blood through an artificial membrane system is a vital tool for evaluating the efficacy of transmission-blocking interventions. however, despite the availability of several published protocols, the smfa remains h ... | 2019 | 30683107 |
| anopheline species composition and the 1014f-genotype in different ecological settings of burkina faso in relation to malaria transmission. | a three-year longitudinal study was conducted in four sentinel sites from different ecological settings in burkina faso, between 2008 and 2010 to identify longitudinal changes in insecticide resistance within anopheles gambiae complex species based on larval collection. during this study, adult mosquitoes were also collected indoor and outdoor using several methods of collection. the present study reports the diversity of malaria vectors and the 1014f-genotype from this adult collection and inve ... | 2019 | 31068189 |
| effect of water source and feed regime on development and phenotypic quality in anopheles gambiae (s.l.): prospects for improved mass-rearing techniques towards release programmes. | in many malaria-endemic sub-saharan countries, insecticide resistance poses a threat to existing mosquito control measures, underscoring the need for complementary control methods such as sterile and/or genetically-modified mosquito release programmes. the sibling species anopheles gambiae and an. coluzzii are responsible for malaria transmission in most of this region. in their natural habitat, these species generally breed in clean, soft water and it is believed that divergent preference in th ... | 2019 | 31060574 |
| evaluation of the residual efficacy of indoor residual spraying with bendiocarb (ficam wp 80) in six health districts in senegal. | from 2011 to 2014, an indoor residual spray (irs) programme for malaria vectors control was implemented in six health districts in senegal. the main objective of the present study was to evaluate the efficacy of bendiocarb (ficam wp 80) sprayed on different wall surfaces and its impact on malaria vectors. the entomological monitoring activities were carried out monthly in five treated sentinel villages and one control untreated village in each district. | 2019 | 31196091 |
| status of insecticide resistance in anopheles gambiae (s.l.) of the gambia. | vector control activities, namely long-lasting insecticidal nets (llin) and indoor residual spraying (irs), have contributed significantly to the decreasing malaria burden observed in the gambia since 2008. nevertheless, insecticide resistance may threaten such success; it is important to regularly assess the susceptibility of local malaria vectors to available insecticides. | 2019 | 31164149 |
| in silico karyotyping of chromosomally polymorphic malaria mosquitoes in the anopheles gambiae complex. | chromosomal inversion polymorphisms play an important role in adaptation to environmental heterogeneities. for mosquito species in the anopheles gambiae complex that are significant vectors of human malaria, paracentric inversion polymorphisms are abundant and are associated with ecologically and epidemiologically important phenotypes. improved understanding of these traits relies on determining mosquito karyotype, which currently depends upon laborious cytogenetic methods whose application is l ... | 2019 | 31391198 |
| a new species in the major malaria vector complex sheds light on reticulated species evolution. | complexes of closely related species provide key insights into the rapid and independent evolution of adaptive traits. here, we described and studied anopheles fontenillei sp.n., a new species in the anopheles gambiae complex that we recently discovered in the forested areas of gabon, central africa. our analysis placed the new taxon in the phylogenetic tree of the an. gambiae complex, revealing important introgression events with other members of the complex. particularly, we detected recent in ... | 2019 | 31611571 |
| windborne long-distance migration of malaria mosquitoes in the sahel. | over the past two decades efforts to control malaria have halved the number of cases globally, yet burdens remain high in much of africa and the elimination of malaria has not been achieved even in areas where extreme reductions have been sustained, such as south africa1,2. studies seeking to understand the paradoxical persistence of malaria in areas in which surface water is absent for 3-8 months of the year have suggested that some species of anopheles mosquito use long-distance migration3. he ... | 2019 | 31578527 |
| micrornas from saliva of anopheline mosquitoes mimic human endogenous mirnas and may contribute to vector-host-pathogen interactions. | during blood feeding haematophagous arthropods inject into their hosts a cocktail of salivary proteins whose main role is to counteract host haemostasis, inflammation and immunity. however, animal body fluids are known to also carry mirnas. to get insights into saliva and salivary gland mirna repertoires of the african malaria vector anopheles coluzzii we used small rna-seq and identified 214 mirnas, including tissue-enriched, sex-biased and putative novel anopheline mirnas. noteworthy, mirnas w ... | 2019 | 30814633 |
| odorant ligands for the co2 receptor in two anopheles vectors of malaria. | exhaled co2 is an important host-seeking cue for anopheles mosquitoes, which is detected by a highly conserved heteromeric receptor consisting of three 7-transmembrane proteins gr22, gr23, and gr24. the co2 receptor neuron has been shown to also respond sensitively to a variety of odorants in aedes aegypti. the detection of co2 is important for upwind navigation and for enhancing the attraction to body heat as well as to skin odorants. the orthologs of the co2 receptor proteins are present in ma ... | 2019 | 30796292 |
| a chordwise offset of the wing-pitch axis enhances rotational aerodynamic forces on insect wings: a numerical study. | most flying animals produce aerodynamic forces by flapping their wings back and forth with a complex wingbeat pattern. the fluid dynamics that underlies this motion has been divided into separate aerodynamic mechanisms of which rotational lift, that results from fast wing pitch rotations, is particularly important for flight control and manoeuvrability. this rotational force mechanism has been modelled using kutta-joukowski theory, which combines the forward stroke motion of the wing with the fa ... | 2019 | 31213176 |
| status of insecticide resistance and its mechanisms in anopheles gambiae and anopheles coluzzii populations from forest settings in south cameroon. | a key factor affecting malaria vector control efforts in cameroon is the rapid expansion of insecticide resistance in anopheles gambiae s.l (an. gambiae) populations; however, mechanisms involved in insecticide resistance in forest mosquito populations are still not well documented yet. the present study was conducted to screen molecular mechanisms conferring insecticide resistance in an. gambiae s.l. populations from the south cameroon forest region. who bioassays were conducted with f0 an. gam ... | 2019 | 31554225 |
| oviposition and development of anopheles coluzzii coetzee and wilkerson in salt water. | anopheles coluzzii is an important vector of malaria in sub-saharan africa particularly of the most dangerous malaria parasite. it completes its life cycle in water and a change in physicochemical properties particularly that of salinity of water may affect egg laying and perhaps the development of eggs to maturity. studies have shown that climate change may alter the transmission of many vector-borne diseases in different parts of the world and global warming will also raise sea levels which wi ... | 2019 | 31687128 |
| mosquito and human hepatocyte infections with plasmodium ovale curtisi and plasmodium ovale wallikeri. | human ovale malaria is caused by the two closely related species, plasmodium ovale curtisi and p. ovale wallikeri. both species are known to relapse from quiescent hepatic forms months or years after the primary infection occurred. although some studies have succeeded in establishing mosquito transmission for ovale malaria, none have specifically described transmission and human hepatocyte infection of both sibling species. | 2019 | 31162595 |
| a combination of metabolic resistance and high frequency of the 1014f kdr mutation is driving pyrethroid resistance in anopheles coluzzii population from guinea savanna of cameroon. | the scale-up in the distribution of long-lasting insecticidal nets (llins) and indoor residual spraying has significantly reduced malaria burden and mortality. however, insecticide resistance, among other factors, is responsible for a recent rebound in malaria transmission in 2015-2016, threatening the progress so far made. as a contribution towards understanding patterns of resistance and its mechanism in the field we characterized a population of anopheles gambiae (s.l.) from gounougou, a guin ... | 2019 | 31133042 |
| malaria mosquitoes use leg push-off forces to control body pitch during take-off. | escaping from a blood host with freshly acquired nutrition for her eggs is one of the most critical actions in the life of a female malaria mosquito. during this take-off, she has to carry a large payload, up to three times her body weight, while avoiding tactile detection by the host. what separates the malaria mosquito from most other insects is that the mosquito pushes off gently with its legs while producing aerodynamic forces with its wings. apart from generating the required forces, the ma ... | 2020 | 31403265 |