Publications

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evaluation of a commercial competitive elisa test kit for the detection of group-specific antibodies to bluetongue virus.the performance of a competitive (c) enzyme-linked immunosorbent assay (elisa) test kit, blueplate special (bps), commercially produced by diagxotics (wilton, ct) for detection of group-specific antibodies to bluetongue virus (btv) was compared with that of an internationally endorsed celisa-i. a total of 1,026 serum samples were tested in this study: 133 samples from 23 calves and 3 sheep experimentally infected with south african isolates of 19 btv serotypes and us isolates of 5 btv serotypes ...19938396982
serosurveillance of viral diseases in korean native goats (capra hircus).a total of 804 goat sera were collected from 144 goat farms in five regions of south korea during a period between 2005 and 2006 and screened for the antibodies of viral pathogens in ruminants. the individual seropositive rates for each virus were 13.7% (110/804) for bovine herpesvirus-1 (bhv-1), 9.5% (76/804) for bovine parainfluenza type-3 virus (pi-3v), 5.5% (44/804) for akabane virus (akav), 13.3% (107/804) for aino virus (ainv), 2.0% (16/804) for chuzan virus (chuv) and 1.0% (8/804) for bov ...200818840974
a single-tube rt-pcr for rapid detection and differentiation of some african isolates of palyam serogroup orbiviruses.a single-tube nested reverse transcriptase (nrt) polymerase chain reaction (nrt-pcr) was developed and evaluated for detection of palyam serogroup orbiviruses ribonucleic acid (rna) in cell cultures and clinical samples. a pair of outer primers (pal1 and pal2), designed from genome segment three of chuzan virus of the palyam viruses serogroup, resulted in amplification of a primary 660-base pair (bp) pcr product. using a pair of internal (nested) primers (pal3 and pal4), the nrt-pcr produced a 3 ...200919500619
development of inactivated trivalent vaccine for the teratogenic aino, akabane and chuzan viruses.aino, akabane and chuzan viruses are arthropod-borne (arbo) viruses transmitted by blood-sucking insects like mosquitoes and culicoides biting midges. these arbovirus infections are mainly associated with abortion, stillbirth and congenital defects in pregnant cattle, sheep and goats, which induces a considerable economic loss in livestock industry. the viruses seem to be widely distributed in southeast asia and australia. as a control strategy, an inactivated trivalent vaccine against aino, aka ...201121411335
host and viral traits predict zoonotic spillover from mammals.the majority of human emerging infectious diseases are zoonotic, with viruses that originate in wild mammals of particular concern (for example, hiv, ebola and sars). understanding patterns of viral diversity in wildlife and determinants of successful cross-species transmission, or spillover, are therefore key goals for pandemic surveillance programs. however, few analytical tools exist to identify which host species are likely to harbour the next human virus, or which viruses can cross species ...201728636590
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